RASGRF2 cDNA ORF clone, Canis lupus familiaris(dog)

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287134 XM_022416755.1
Latest version!
Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe35549 XM_014111758.2
Latest version!
Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OCe35549 XM_014111758.1 Canis lupus familiaris Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287134
    Clone ID Related Accession (Same CDS sequence) XM_022416755.1
    Accession Version XM_022416755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC
    CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC
    CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC
    GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC
    GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG
    GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA
    GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA
    GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA
    GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG
    TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT
    ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC
    CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG
    AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG
    TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG
    ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG
    CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC
    AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC
    CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT
    CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG
    GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG
    GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC
    ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT
    CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA
    CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG
    GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT
    AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT
    TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG
    AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT
    GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA
    GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC
    TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT
    TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA
    AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG
    TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC
    TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG
    AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG
    ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA
    CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC
    CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA
    CGAAGTATTC AAAGAGGATT TAACAACACT GAGAGGACAT GTGACAAAGA GTTTATTATT
    CGAAGAACAG CCACCAATCG AGTCCTGAAT GTCCTCCGTC ACTGGGTCTC AAAGCACGCA
    CAGGATTTTG AACTCAACAA CGAACTAAAG ATGAATGTCC TAAATTTGCT AGAAGAAGTT
    TTACGAGACC CAGACCTTCT TCCCCAGGAA AGGAAAGCCA CGGCGAATAT CCTGAGGGCC
    CTTTCACAAG ATGATCAAGA TGACATCCAC CTAAAATTAG AGGATATAAT TCAACTGACA
    GACTGTCCGA AGGCTGAGTG CTTTGAGACC TTGTCAGCCA TGGAGCTGGC TGAGCAGATC
    ACCCTCCTGG ACCACATCAT CTTCAGAAGC ATTCCCTACG AAGAATTTCT TGGGCAGGGG
    TGGATGAAGC TGGATAAAAA TGAAAGAACA CCTTACATTA TGAAAACCAG CCAACACTTC
    AATGATATGA GTAACCTGGT GGCCTCCCAG ATAATGAACT ATGCGGACGT CAGCTCGAGG
    GCCAACGCGA TAGAGAAGTG GGTGGCCGTG GCGGACATCT GCCGATGCCT GCACAACTAC
    AACGGCGTGC TGGAGATCAC CTCAGCCTTA AACAGAAGTG CCATCTACCG GCTGAAGAGA
    ACCTGGGCCA AGGTGTCCAA GCAGACAAAA GCTCTAATGG ACAAGCTTCA GAAGACCGTT
    TCATCTGAAG GAAGATTTAA AAATCTCAGA GAAACCCTTA AAAACTGTAA CCCCCCAGCT
    GTTCCTTATC TGGGGATGTA CCTGACAGAC CTGGCCTTTA TTGAAGAAGG AACACCAAAC
    TTTACTGAGG AAGGCCTCGT TAATTTCTCC AAAATGAGAA TGATATCACA CATCATCAGA
    GAGATACGCC AATTCCAGCA GACTTGCTAT CGAATAGACC ATCAGCCAAA GGGAGAGCTC
    TGCTTTACAA GAAGAAACAG CAAAATACAG AGTTCTTGGT ACCTGAGCCT ATTGCACCTG
    CTGGTTTCTC TTGGAGGCAT GAAGTATATT GATGCTGACA GTGAAAAAAA AAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272463.1
    CDS101..3637
    Translation

    Target ORF information:

    RefSeq Version XM_022416755.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC
    CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC
    CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC
    GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC
    GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG
    GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA
    GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA
    GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA
    GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG
    TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT
    ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC
    CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG
    AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG
    TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG
    ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG
    CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC
    AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC
    CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT
    CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG
    GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG
    GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC
    ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT
    CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA
    CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG
    GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT
    AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT
    TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG
    AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT
    GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA
    GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC
    TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT
    TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA
    AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG
    TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC
    TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG
    AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG
    ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA
    CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC
    CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA
    CGAAGTATTC AAAGAGGATT TAACAACACT GAGAGGACAT GTGACAAAGA GTTTATTATT
    CGAAGAACAG CCACCAATCG AGTCCTGAAT GTCCTCCGTC ACTGGGTCTC AAAGCACGCA
    CAGGATTTTG AACTCAACAA CGAACTAAAG ATGAATGTCC TAAATTTGCT AGAAGAAGTT
    TTACGAGACC CAGACCTTCT TCCCCAGGAA AGGAAAGCCA CGGCGAATAT CCTGAGGGCC
    CTTTCACAAG ATGATCAAGA TGACATCCAC CTAAAATTAG AGGATATAAT TCAACTGACA
    GACTGTCCGA AGGCTGAGTG CTTTGAGACC TTGTCAGCCA TGGAGCTGGC TGAGCAGATC
    ACCCTCCTGG ACCACATCAT CTTCAGAAGC ATTCCCTACG AAGAATTTCT TGGGCAGGGG
    TGGATGAAGC TGGATAAAAA TGAAAGAACA CCTTACATTA TGAAAACCAG CCAACACTTC
    AATGATATGA GTAACCTGGT GGCCTCCCAG ATAATGAACT ATGCGGACGT CAGCTCGAGG
    GCCAACGCGA TAGAGAAGTG GGTGGCCGTG GCGGACATCT GCCGATGCCT GCACAACTAC
    AACGGCGTGC TGGAGATCAC CTCAGCCTTA AACAGAAGTG CCATCTACCG GCTGAAGAGA
    ACCTGGGCCA AGGTGTCCAA GCAGACAAAA GCTCTAATGG ACAAGCTTCA GAAGACCGTT
    TCATCTGAAG GAAGATTTAA AAATCTCAGA GAAACCCTTA AAAACTGTAA CCCCCCAGCT
    GTTCCTTATC TGGGGATGTA CCTGACAGAC CTGGCCTTTA TTGAAGAAGG AACACCAAAC
    TTTACTGAGG AAGGCCTCGT TAATTTCTCC AAAATGAGAA TGATATCACA CATCATCAGA
    GAGATACGCC AATTCCAGCA GACTTGCTAT CGAATAGACC ATCAGCCAAA GGGAGAGCTC
    TGCTTTACAA GAAGAAACAG CAAAATACAG AGTTCTTGGT ACCTGAGCCT ATTGCACCTG
    CTGGTTTCTC TTGGAGGCAT GAAGTATATT GATGCTGACA GTGAAAAAAA AAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35549
    Clone ID Related Accession (Same CDS sequence) XM_014111758.2 , XM_014111758.1
    Accession Version XM_014111758.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC
    CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC
    CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC
    GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC
    GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG
    GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA
    GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA
    GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA
    GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG
    TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT
    ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC
    CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG
    AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG
    TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG
    ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG
    CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC
    AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC
    CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT
    CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG
    GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG
    GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC
    ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT
    CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA
    CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG
    GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT
    AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT
    TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG
    AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT
    GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA
    GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC
    TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT
    TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA
    AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG
    TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC
    TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG
    AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG
    ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA
    CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC
    CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA
    CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT
    TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT
    CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT
    GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC
    AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC
    CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA
    CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC
    CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC
    ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT
    GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC
    AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA
    AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC
    TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG
    GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA
    GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG
    ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT
    CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG
    ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT
    TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT
    TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA
    ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG
    TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967233.1
    CDS100..3852
    Translation

    Target ORF information:

    RefSeq Version XM_014111758.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111758.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC
    CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC
    CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC
    GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC
    GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG
    GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA
    GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA
    GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA
    GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG
    TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT
    ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC
    CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG
    AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG
    TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG
    ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG
    CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC
    AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC
    CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT
    CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG
    GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG
    GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC
    ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT
    CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA
    CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG
    GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT
    AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT
    TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG
    AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT
    GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA
    GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC
    TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT
    TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA
    AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG
    TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC
    TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG
    AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG
    ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA
    CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC
    CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA
    CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT
    TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT
    CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT
    GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC
    AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC
    CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA
    CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC
    CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC
    ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT
    GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC
    AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA
    AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC
    TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG
    GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA
    GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG
    ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT
    CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG
    ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT
    TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT
    TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA
    ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG
    TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35549
    Clone ID Related Accession (Same CDS sequence) XM_014111758.2 , XM_014111758.1
    Accession Version XM_014111758.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ras-specific guanine nucleotide-releasing factor 2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726052.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC
    CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC
    CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC
    GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC
    GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG
    GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA
    GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA
    GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA
    GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG
    TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT
    ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC
    CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG
    AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG
    TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG
    ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG
    CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC
    AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC
    CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT
    CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG
    GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG
    GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC
    ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT
    CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA
    CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG
    GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT
    AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT
    TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG
    AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT
    GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA
    GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC
    TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT
    TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA
    AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG
    TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC
    TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG
    AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG
    ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA
    CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC
    CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA
    CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT
    TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT
    CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT
    GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC
    AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC
    CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA
    CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC
    CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC
    ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT
    GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC
    AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA
    AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC
    TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG
    GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA
    GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG
    ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT
    CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG
    ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT
    TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT
    TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA
    ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG
    TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967233.1
    CDS1..3753
    Translation

    Target ORF information:

    RefSeq Version XM_014111758.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCAGAAGA GCGTGCGCTA CAACGAGGGG CACGCCCTGC ACCTGGCCTT CCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGA AAAAATGAGC AAGAGGGCGG CCGAGGCGAG CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA CCAGAATGTG CTCTTCTACT TCGAGGGCGA GCAGAGCGGC
    CGCCCGGCGG GCATGTACCT CCTGGAGGGC TGCAGCTGCG AGCGCACGCC CGCGCCGCCC
    CGGGCCGGGG TCGGGCCGCC CGGCGCCCGG GACGCGCTGG ACAAGCAGTA CTACTTTACC
    GTTCTTTTTG GCCATGAAGG TCAGAAACCT CTGGAGCTGC GCTGTGAGGA GGAGCAGGAC
    GGTAAAGAGT GGATGGAGGC CATTCACCAA GCCAGTTATG CAGACATTTT GATTGAGAGG
    GAAGTGTTAA TGCAGAAGTA CATTCATCTA GTTCAGATCG TAGAGACAGA AAAAATTGCA
    GCTAACCAGC TCCGGCATCA ACTTGAAGAT CAAGATACAG AAATTGAAAG ACTTAAATCA
    GAGATTATCG CTCTTAATAA AACCAAGGAA CGAATGCGGC CTTACCAAAG CAATCAAGAA
    GATGAGGATC CAGACATCAA GAAGATTAAA AAGGTTCAGA GCTTCATGAG AGGATGGTTG
    TGTAGAAGGA AATGGAAGAC TATTGTGCAG GATTACATTT GTTCTCCTCA TGCTGAAAGT
    ATGAGGAAGA GAAACCAGAT TGTGTTCACC ATGGTTGAAG CCGAGTCAGA GTATGTTCAC
    CAGCTCTACA TCCTGGTCAA CGGCTTCCTC CGGCCCCTGC GGATGGCGGC CAGCTCCAAG
    AAGCCTCCCA TCAGCCATGA TGATGTCAGC AGCATTTTCC TCAACAGTGA AACGATTATG
    TTTCTTCATG AAATATTTCA CCAAGGACTG AAGGCGAGGA TAGCAAACTG GCCTACTTTG
    ATTTTAGCTG ATCTGTTTGA TATTTTGCTC CCCATGTTGA ACATTTATCA AGAATTTGTG
    CGTAATCACC AGTACAGCCT CCAAGTGCTT GCCAATTGTA AGCAAAACAG AGATTTTGAC
    AAACTCTTAA AACAGTATGA AGCCAACCCT GCCTGTGAGG GGAGGATGCT GGAAACGTTC
    CTGACCTATC CAATGTTTCA GATCCCCAGG TATATCATCA CACTTCATGA ACTCCTAGCT
    CATACACCTC ATGAGCATGT GGAAAGGAAA AGTCTGGAGT TTGCTAAATC GAAACTAGAG
    GAACTGTCCA GGATAATGCA CGATGAAGTG AGTGACACTG AAAACATAAG GAAGAACCTG
    GCCATAGAAA GAATGATCGT AGAGGGCTGT GATATTTTGC TGGACACCAG CCAAACTTTC
    ATCCGCCAAG GTTCTCTTAT TCAAGTACCT TCTGTTGAGA GGGGAAAACT TAGTAAAGTT
    CGCCTGGGTT CATTGTCTTT GAAAAAGGAA GGAGAAAGAC AGTGCTTCTT ATTTACAAAA
    CACTTTTTAA TTTGTACAAG AAGTTCAGGA GGAAAGCTAC ATCTGCTCAA GACAGGTGGG
    GTTCTCTCTC TAATAGAATG TACATTGATT GAGGAGCCCG ATGCAAGTGA TGATGACTCT
    AAAGGTTCTG GGCAAGTGTT TGGACACCTG GATTTTAAAA TAGTAGTGGA GCCTCCTGAT
    TCTGCCCCTT TCACTGTAGT CTTGTTAGCA CCTTCACGCC AAGAGAAGGC TGCCTGGACG
    AGCGACATAA GTCAGTGTGT GGACAATATA CGGTGTAATG GTTTAATGAC TATAGTATTT
    GAGGAGAATT CCAAAGTTAC TGTGCCCCAT ATGATTAAGT CTGATGCCCG TCTTCACAAA
    GATGACACCG ACATTTGCTT CAGTAAAACA CTCAACTCCT GCAAAGTGCC CCAGATCCGC
    TATGCCAGCG TGGAGCGCCT CCTGGAGCGG CTGACAGACT TACGGTTTCT CAGTATCGAT
    TTCCTCAATA CCTTTCTGCA CACCTATCGT ATTTTCACTA CGGCAGCTGT GGTGCTGGCA
    AAACTTTCTG ACATATACAA GAGGCCTTTT ACCTCCATTC CTGTCAGGTC ATTGGAATTG
    TTTTTTGCTA CCAGCCAGAA CAATAGAGGT GAACATTTGG TGGATGGCAA ATCCCCACGC
    TTGTGTCGCA AATTCTCTTC CCCCCCACCG CTGGCTGTGT CCAGGACGTC CTCCCCAGTG
    AGGGCCAGAA AGCTGTCCTT GACTTCTCCA TTGAACTCAA GGATAGGAGC GTTGGACCTG
    ACCACCTGTT CAGCATCCAG TAGTCCTACC GCCACCCACA GCCCCGCTGC CTCCCCACCA
    CCGCACACCG GGAAAGTACC GTTGGATCTT AGCAGAGGCC TTCCTTCTCC AGAGCAAAGC
    CCGGGATCTG TAGAAGAGCA CGTGGATAAC CCCCGCATGG ATCTGTGTAA CAAGCTAAAA
    CGAAGTATTC AAAGAGCTGT CCTTGAGTCT GTCCCTGTGG ACCGAGCTGG AGTGGAAGGT
    TCCCCGGCGG TGGACAGCAC AGACCTTTCA CCTTGCCGAT CACCTTCCAC GCCTCAGCAT
    CTCCGCTATC GCCAGCCTGT AGGACAGACG GCTGACAACT CCCACTGCTC TGTTTCGCCT
    GCTTCTGCTT TTGCAATTGC CACGGCGGCT GCAGGACATG GAAGTCCACC AGGATTTAAC
    AACACTGAGA GGACATGTGA CAAAGAGTTT ATTATTCGAA GAACAGCCAC CAATCGAGTC
    CTGAATGTCC TCCGTCACTG GGTCTCAAAG CACGCACAGG ATTTTGAACT CAACAACGAA
    CTAAAGATGA ATGTCCTAAA TTTGCTAGAA GAAGTTTTAC GAGACCCAGA CCTTCTTCCC
    CAGGAAAGGA AAGCCACGGC GAATATCCTG AGGGCCCTTT CACAAGATGA TCAAGATGAC
    ATCCACCTAA AATTAGAGGA TATAATTCAA CTGACAGACT GTCCGAAGGC TGAGTGCTTT
    GAGACCTTGT CAGCCATGGA GCTGGCTGAG CAGATCACCC TCCTGGACCA CATCATCTTC
    AGAAGCATTC CCTACGAAGA ATTTCTTGGG CAGGGGTGGA TGAAGCTGGA TAAAAATGAA
    AGAACACCTT ACATTATGAA AACCAGCCAA CACTTCAATG ATATGAGTAA CCTGGTGGCC
    TCCCAGATAA TGAACTATGC GGACGTCAGC TCGAGGGCCA ACGCGATAGA GAAGTGGGTG
    GCCGTGGCGG ACATCTGCCG ATGCCTGCAC AACTACAACG GCGTGCTGGA GATCACCTCA
    GCCTTAAACA GAAGTGCCAT CTACCGGCTG AAGAGAACCT GGGCCAAGGT GTCCAAGCAG
    ACAAAAGCTC TAATGGACAA GCTTCAGAAG ACCGTTTCAT CTGAAGGAAG ATTTAAAAAT
    CTCAGAGAAA CCCTTAAAAA CTGTAACCCC CCAGCTGTTC CTTATCTGGG GATGTACCTG
    ACAGACCTGG CCTTTATTGA AGAAGGAACA CCAAACTTTA CTGAGGAAGG CCTCGTTAAT
    TTCTCCAAAA TGAGAATGAT ATCACACATC ATCAGAGAGA TACGCCAATT CCAGCAGACT
    TGCTATCGAA TAGACCATCA GCCAAAGGGA GAGCTCTGCT TTACAAGAAG AAACAGCAAA
    ATACAGAGTT CTTGGTACCT GAGCCTATTG CACCTGCTGG TTTCTCTTGG AGGCATGAAG
    TATATTGATG CTGACAGTGA AAAAAAAAAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.