RASGRF2 cDNA ORF clone, Gallus gallus(chicken)

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa21740 XM_004949460.3
Latest version!
Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa21741 XM_003643077.4
Latest version!
Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa48314 XM_015280709.2
Latest version!
Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa48315 XM_015280710.2
Latest version!
Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa48316 XM_015280711.2
Latest version!
Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OGa48314 XM_015280709.1 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa21740 XM_004949460.2 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa21741 XM_003643077.3 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa48315 XM_015280710.1 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa48316 XM_015280711.1 Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa21740
    Clone ID Related Accession (Same CDS sequence) XM_004949460.3 , XM_004949460.2
    Accession Version XM_004949460.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004949460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC
    ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT
    CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT
    GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC
    AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC
    CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG
    CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA
    CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT
    ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT
    GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC
    AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG
    AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC
    TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA
    GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA
    GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG
    GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT
    CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG
    ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT
    TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC
    TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC
    ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004949517.1
    CDS293..4012
    Translation

    Target ORF information:

    RefSeq Version XM_004949460.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004949460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC
    ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT
    CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT
    GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC
    AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC
    CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG
    CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA
    CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT
    ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT
    GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC
    AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG
    AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC
    TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA
    GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA
    GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG
    GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT
    CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG
    ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT
    TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC
    TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC
    ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa21740
    Clone ID Related Accession (Same CDS sequence) XM_004949460.3 , XM_004949460.2
    Accession Version XM_004949460.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004949460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC
    ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT
    CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT
    GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC
    AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC
    CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG
    CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA
    CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT
    ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT
    GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC
    AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG
    AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC
    TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA
    GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA
    GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG
    GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT
    CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG
    ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT
    TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC
    TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC
    ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004949517.1
    CDS301..4020
    Translation

    Target ORF information:

    RefSeq Version XM_004949460.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004949460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGCAGT TCTGGAATCA GTGTCAGTGG ACAGAACTAT TCCTGAAAGC
    ACCTCGGCAG TGGATACCAC AGAACTTTCC CCGTGTAGAT CCCCTTCAAC ACCACGTCAT
    CTCCGCTATC GTCAATCAGG AGTGCAAACT GCTGACAACA GTCACTGTGC TGTTTCTCCT
    GCATCAGCTT TTGCTATTGC TACAGCTGCA GCAGGGCATG GGAGTCCGCC AGGTTTTAAC
    AATTCTGAAC GAATGTGTGA CAAAGAGTTC ATAATACGCA GAGCAGCCAC TAATCGTGTC
    CTGAATGTCT TACGTCACTG GGTCTCCAAG CACTCTCAGG ATTTTGAACT AAACAATGAG
    CTGAAAATGA ATGTGTTAAA TTTGCTGGAA GAAGTTTTGA GAGACCCTGA CCTTCTACCA
    CAAGAAAGGA AAGCTACTGC AAATATACTA AGGGCACTTT CTCAGGACGA TCAAGATGAT
    ACCCATTTAA AAATAGAGGA TATCATTCAG ATGTCAGAAT GTCCAAAACC GGAGTGCTTT
    GAGACTTTGT CTGCCATGGA GCTTGCAGAG CAAATAACTC TTTTAGACCA CATAGTTTTC
    AGAAGCATAC CCTATGAAGA GTTTCTTGGG CAGGGCTGGA TGAAACTGGA TAAAAATGAG
    AGAACACCCT ATATTATGAA AACTAGTCAA CATTTTAATG ATATGAGTAA CCTTGTTGCC
    TCCCAAATAA TGAATTATGC TGATGTCAGC TCCCGGGCTA ACTCAATTGA AAAGTGGGTA
    GCAGTAGCTG ATATTTGTCG ATGTATGCAC AATTACAATG GTGTGTTAGA AATCACGTCA
    GCCTTGAACA GAAGTGCAAT CTACAGACTG AAGAAAACTT GGGCTAAGGT ATCCAAACAG
    GCAAAAGCAC TCATGGACAA GCTTCAGAAG ACTGTGTCAT CTGAAGGAAG ATTTAAAAAT
    CTTAGAGAAA CACTCAAAAA TTGTAACCCA CCTGCTGTTC CATACCTTGG AATGTATTTG
    ACAGATCTAG CATTTATTGA AGAAGGAACA CCAAACTTCA CTGAGGAAGG CCTTGTTAAT
    TTTTCCAAGA TGAGAATGAT CTCACACATT ATCAGGGAAA TACGCCAGTT CCAGCAGACC
    TCCTACCGCA TAGAACATCA GCAGAAGGTC ACTCACTACT TACTCGACAA GGCACTCATC
    ATAGATGAAG ATACTTTGTA TGAGCTTTCC CTGAAGCTTG AACCCAGACT CCCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa21741
    Clone ID Related Accession (Same CDS sequence) XM_003643077.3 , XM_003643077.4
    Accession Version XM_003643077.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003643077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC
    TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC
    CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA
    TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT
    TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG
    AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG
    AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA
    GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC
    CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG
    ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA
    AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA
    ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC
    CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA
    GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC
    TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA
    AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT
    AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA
    GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT
    TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC
    TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA
    GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003643125.1
    CDS302..4018
    Translation

    Target ORF information:

    RefSeq Version XM_003643077.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003643077.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC
    TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC
    CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA
    TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT
    TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG
    AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG
    AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA
    GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC
    CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG
    ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA
    AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA
    ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC
    CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA
    GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC
    TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA
    AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT
    AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA
    GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT
    TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC
    TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA
    GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa21741
    Clone ID Related Accession (Same CDS sequence) XM_003643077.3 , XM_003643077.4
    Accession Version XM_003643077.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003643077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC
    TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC
    CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA
    TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT
    TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG
    AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG
    AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA
    GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC
    CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG
    ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA
    AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA
    ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC
    CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA
    GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC
    TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA
    AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT
    AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA
    GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT
    TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC
    TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA
    GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003643125.1
    CDS293..4009
    Translation

    Target ORF information:

    RefSeq Version XM_003643077.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003643077.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGAT TCCTAGATAC ATAATAACAC TTCATGAACT CCTGGCTCAC
    ACACCACATG AACACGTTGA ACGAAAAAGC CTTGAATTTG CAAAATCTAA ACTAGAAGAA
    CTTTCAAGGG TGATGCATGA TGAAGTGAGT GACACGGAAA ACATCCGGAA GAATCTGGCC
    ATAGAAAGAA CAATAGTAGA AGGCTGTGAT ATATTGCTAG ATACTAGTCA AACTTTTATA
    CGCCAAGGTT CCCTTATTCA AGTCCCTTCA GTTGAAAGGG GAAAACTTAG CAAAGTTCGT
    CTGGGATCAT TGTCTTTGAA AAAAGAAGGA GAAAGGCAAT GCTTCTTATT TACAAAACAT
    TTTTTAATTT GTACAAGAAG TTCAGGAGGA AAACTGCATC TACTTAAGAC AGGAGGTGTT
    CTGTCGTTGA TAGAGTGCAC ATTGATTGAG GAGACAGATG CAACTGATGA TGACTCAAAA
    AGCACTGGAC AGGTGTTTGG ACACCTTGAT TTCAAGCTTG TAGTTGAACC TACAGATGCT
    CCTCCTTTTA CTGTTGTCCT CTTGGCTCCT TCACGTCAAG AGAAAGCTGC ATGGACAAGT
    GATATAAGTC AGTGCATTGA TAACATAAGG TGCAATGGTT TAATGACCAT AGTGTTTGAA
    GAAAATTCTA AAGTCACTGT GCCACATATG ATAAAGTCTG ATGCACGTCT TCATAGAGAT
    GACATTGATA TCTGCTTCAG CAAAACTCTA AATTCCTGCA AAGTACCTCA AATCCGTTAT
    GCAAGTGTTG AACGCCTTTT GGAGAGGCTA ACAGACTTGC GGTTTCTGAG CATTGATTTC
    CTGAATACCT TCCTGCATAC TTACCGCATA TTTACTACTG CAGCTGTTGT ACTGGAAAAA
    CTTTCAGACA TATACAGACG GCCTTTCACT TCCATTCCAG TTAGGGCTTT GGAGTTATTT
    TTTGCTACCA GTCAGAACAA CAGAGGAGAG TACCTTGCTG ATGCTAAGTC ACCGCATCTC
    TGTCGCAAAT TTTCTTCTCC CCCTCCACTG TCACTTTCCA GAACATCCTC ACCTGTCAGA
    ACTCGAAAAT TGTCATTGAC TTCACCACTG AATTCAAGAA TTGGTGCCCT TGATCTTTCT
    ACTTCATCTG TGGCAAGCAG TCCTACCTCA ACCTATAGTC CAGCCACCTC TCCTCCACCA
    ACAGGTAGCA AAGTACCTCT GGACCTTAGC AAAGGGCCCT CCTCTCCTGA GCAAAGCCCT
    GGCTCCATAG AGGACAGCTT GGAGAACCCT CGCATTGACC TGTGTAACAA ACTGAGGAGA
    AGCATTCGAA GAGCAGTTCT GGAATCAGTG TCAGTGGACA GAACTATTCC TGAAAGCACC
    TCGGCAGTGG ATACCACAGA ACTTTCCCCG TGTAGATCCC CTTCAACACC ACGTCATCTC
    CGCTATCGTC AATCAGGAGT GCAAACTGCT GACAACAGTC ACTGTGCTGT TTCTCCTGCA
    TCAGCTTTTG CTATTGCTAC AGCTGCAGCA GGGCATGGGA GTCCGCCAGG TTTTAACAAT
    TCTGAACGAA TGTGTGACAA AGAGTTCATA ATACGCAGAG CAGCCACTAA TCGTGTCCTG
    AATGTCTTAC GTCACTGGGT CTCCAAGCAC TCTCAGGATT TTGAACTAAA CAATGAGCTG
    AAAATGAATG TGTTAAATTT GCTGGAAGAA GTTTTGAGAG ACCCTGACCT TCTACCACAA
    GAAAGGAAAG CTACTGCAAA TATACTAAGG GCACTTTCTC AGGACGATCA AGATGATACC
    CATTTAAAAA TAGAGGATAT CATTCAGATG TCAGAATGTC CAAAACCGGA GTGCTTTGAG
    ACTTTGTCTG CCATGGAGCT TGCAGAGCAA ATAACTCTTT TAGACCACAT AGTTTTCAGA
    AGCATACCCT ATGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAGAGA
    ACACCCTATA TTATGAAAAC TAGTCAACAT TTTAATGATA TGAGTAACCT TGTTGCCTCC
    CAAATAATGA ATTATGCTGA TGTCAGCTCC CGGGCTAACT CAATTGAAAA GTGGGTAGCA
    GTAGCTGATA TTTGTCGATG TATGCACAAT TACAATGGTG TGTTAGAAAT CACGTCAGCC
    TTGAACAGAA GTGCAATCTA CAGACTGAAG AAAACTTGGG CTAAGGTATC CAAACAGGCA
    AAAGCACTCA TGGACAAGCT TCAGAAGACT GTGTCATCTG AAGGAAGATT TAAAAATCTT
    AGAGAAACAC TCAAAAATTG TAACCCACCT GCTGTTCCAT ACCTTGGAAT GTATTTGACA
    GATCTAGCAT TTATTGAAGA AGGAACACCA AACTTCACTG AGGAAGGCCT TGTTAATTTT
    TCCAAGATGA GAATGATCTC ACACATTATC AGGGAAATAC GCCAGTTCCA GCAGACCTCC
    TACCGCATAG AACATCAGCA GAAGGTCACT CACTACTTAC TCGACAAGGC ACTCATCATA
    GATGAAGATA CTTTGTATGA GCTTTCCCTG AAGCTTGAAC CCAGACTCCC CGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48314
    Clone ID Related Accession (Same CDS sequence) XM_015280709.1 , XM_015280709.2
    Accession Version XM_015280709.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA
    AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT
    GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG
    GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG
    GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA
    GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG
    AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT
    GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT
    CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA
    ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC
    AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA
    AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT
    GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG
    ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT
    CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG
    TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136195.1
    CDS303..4124
    Translation

    Target ORF information:

    RefSeq Version XM_015280709.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA
    AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT
    GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG
    GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG
    GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA
    GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG
    AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT
    GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT
    CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA
    ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC
    AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA
    AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT
    GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG
    ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT
    CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG
    TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48314
    Clone ID Related Accession (Same CDS sequence) XM_015280709.1 , XM_015280709.2
    Accession Version XM_015280709.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280709.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA
    AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT
    GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG
    GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG
    GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA
    GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG
    AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT
    GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT
    CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA
    ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC
    AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA
    AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT
    GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG
    ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT
    CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG
    TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136195.1
    CDS295..4116
    Translation

    Target ORF information:

    RefSeq Version XM_015280709.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280709.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGATTTTGAA CTAAACAATG AGCTGAAAAT GAATGTGTTA
    AATTTGCTGG AAGAAGTTTT GAGAGACCCT GACCTTCTAC CACAAGAAAG GAAAGCTACT
    GCAAATATAC TAAGGGCACT TTCTCAGGAC GATCAAGATG ATACCCATTT AAAAATAGAG
    GATATCATTC AGATGTCAGA ATGTCCAAAA CCGGAGTGCT TTGAGACTTT GTCTGCCATG
    GAGCTTGCAG AGCAAATAAC TCTTTTAGAC CACATAGTTT TCAGAAGCAT ACCCTATGAA
    GAGTTTCTTG GGCAGGGCTG GATGAAACTG GATAAAAATG AGAGAACACC CTATATTATG
    AAAACTAGTC AACATTTTAA TGATATGAGT AACCTTGTTG CCTCCCAAAT AATGAATTAT
    GCTGATGTCA GCTCCCGGGC TAACTCAATT GAAAAGTGGG TAGCAGTAGC TGATATTTGT
    CGATGTATGC ACAATTACAA TGGTGTGTTA GAAATCACGT CAGCCTTGAA CAGAAGTGCA
    ATCTACAGAC TGAAGAAAAC TTGGGCTAAG GTATCCAAAC AGGCAAAAGC ACTCATGGAC
    AAGCTTCAGA AGACTGTGTC ATCTGAAGGA AGATTTAAAA ATCTTAGAGA AACACTCAAA
    AATTGTAACC CACCTGCTGT TCCATACCTT GGAATGTATT TGACAGATCT AGCATTTATT
    GAAGAAGGAA CACCAAACTT CACTGAGGAA GGCCTTGTTA ATTTTTCCAA GATGAGAATG
    ATCTCACACA TTATCAGGGA AATACGCCAG TTCCAGCAGA CCTCCTACCG CATAGAACAT
    CAGCAGAAGG TCACTCACTA CTTACTCGAC AAGGCACTCA TCATAGATGA AGATACTTTG
    TATGAGCTTT CCCTGAAGCT TGAACCCAGA CTCCCCGCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48315
    Clone ID Related Accession (Same CDS sequence) XM_015280710.2 , XM_015280710.1
    Accession Version XM_015280710.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT
    CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA
    GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA
    GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT
    ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC
    AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG
    GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT
    TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA
    AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT
    ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG
    CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG
    TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT
    GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA
    TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC
    TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG
    CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT
    TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA
    GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC
    TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG
    CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT
    GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136196.1
    CDS295..4113
    Translation

    Target ORF information:

    RefSeq Version XM_015280710.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280710.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT
    CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA
    GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA
    GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT
    ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC
    AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG
    GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT
    TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA
    AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT
    ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG
    CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG
    TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT
    GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA
    TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC
    TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG
    CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT
    TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA
    GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC
    TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG
    CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT
    GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48315
    Clone ID Related Accession (Same CDS sequence) XM_015280710.2 , XM_015280710.1
    Accession Version XM_015280710.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT
    CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA
    GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA
    GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT
    ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC
    AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG
    GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT
    TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA
    AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT
    ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG
    CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG
    TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT
    GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA
    TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC
    TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG
    CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT
    TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA
    GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC
    TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG
    CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT
    GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136196.1
    CDS303..4121
    Translation

    Target ORF information:

    RefSeq Version XM_015280710.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGTT
    CTGGAATCAG TGTCAGTGGA CAGAACTATT CCTGAAAGCA CCTCGGCAGT GGATACCACA
    GAACTTTCCC CGTGTAGATC CCCTTCAACA CCACGTCATC TCCGCTATCG TCAATCAGGA
    GTGCAAACTG CTGACAACAG TCACTGTGCT GTTTCTCCTG CATCAGCTTT TGCTATTGCT
    ACAGCTGCAG CAGGGCATGG GAGTCCGCCA GGTTTTAACA ATTCTGAACG AATGTGTGAC
    AAAGAGTTCA TAATACGCAG AGCAGCCACT AATCGTGTCC TGAATGTCTT ACGTCACTGG
    GTCTCCAAGC ACTCTCAGGA TTTTGAACTA AACAATGAGC TGAAAATGAA TGTGTTAAAT
    TTGCTGGAAG AAGTTTTGAG AGACCCTGAC CTTCTACCAC AAGAAAGGAA AGCTACTGCA
    AATATACTAA GGGCACTTTC TCAGGACGAT CAAGATGATA CCCATTTAAA AATAGAGGAT
    ATCATTCAGA TGTCAGAATG TCCAAAACCG GAGTGCTTTG AGACTTTGTC TGCCATGGAG
    CTTGCAGAGC AAATAACTCT TTTAGACCAC ATAGTTTTCA GAAGCATACC CTATGAAGAG
    TTTCTTGGGC AGGGCTGGAT GAAACTGGAT AAAAATGAGA GAACACCCTA TATTATGAAA
    ACTAGTCAAC ATTTTAATGA TATGAGTAAC CTTGTTGCCT CCCAAATAAT GAATTATGCT
    GATGTCAGCT CCCGGGCTAA CTCAATTGAA AAGTGGGTAG CAGTAGCTGA TATTTGTCGA
    TGTATGCACA ATTACAATGG TGTGTTAGAA ATCACGTCAG CCTTGAACAG AAGTGCAATC
    TACAGACTGA AGAAAACTTG GGCTAAGGTA TCCAAACAGG CAAAAGCACT CATGGACAAG
    CTTCAGAAGA CTGTGTCATC TGAAGGAAGA TTTAAAAATC TTAGAGAAAC ACTCAAAAAT
    TGTAACCCAC CTGCTGTTCC ATACCTTGGA ATGTATTTGA CAGATCTAGC ATTTATTGAA
    GAAGGAACAC CAAACTTCAC TGAGGAAGGC CTTGTTAATT TTTCCAAGAT GAGAATGATC
    TCACACATTA TCAGGGAAAT ACGCCAGTTC CAGCAGACCT CCTACCGCAT AGAACATCAG
    CAGAAGGTCA CTCACTACTT ACTCGACAAG GCACTCATCA TAGATGAAGA TACTTTGTAT
    GAGCTTTCCC TGAAGCTTGA ACCCAGACTC CCCGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48316
    Clone ID Related Accession (Same CDS sequence) XM_015280711.2 , XM_015280711.1
    Accession Version XM_015280711.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA
    GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT
    GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136197.1
    CDS302..3352
    Translation

    Target ORF information:

    RefSeq Version XM_015280711.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280711.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA
    GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT
    GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48316
    Clone ID Related Accession (Same CDS sequence) XM_015280711.2 , XM_015280711.1
    Accession Version XM_015280711.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product ras-specific guanine nucleotide-releasing factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764321.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA
    GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT
    GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015136197.1
    CDS310..3360
    Translation

    Target ORF information:

    RefSeq Version XM_015280711.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCAGAAGA GCGTGCGGTA CAACGAGGGG CACGCCCTAT ACCTGGCGCT GCTGGCCCGC 
    AAGGAGGGCA CCAAGCGCGG CTACCTGAGC AAGAGGACGG CCGAGGCCAA CCGCTGGCAC
    GAGAAGTGGT TCGCCCTCTA TCAGAACGTC CTCTTCTACT TCGAGAGCGA GCAGAGCGCC
    CGGCCCGCCG GCATCTACAT GCTGGAGGGC TGCAGCTGCG AGCGGGCGCC GGCCCCCAAG
    GGCGCGGCCG CCGGCAGCGC GAAGGACGCG GCGCTGGACA AGCAGTATTA CTTTACTGTT
    TTGTTCGGCC ATGAAGGGCA GAAGCCACTG GAGCTCCGTT GTGAGGACGA AGGTGATGGA
    GATGAATGGG TGGAAGCTAT TCACCAGGCT AGTTATTCAG ATATTCTGAT TGAAAGAGAA
    GTACTGATGC AGAAGTACAT TCATCTTGTC CAAATTGTAG AGACTGAAAA GATTGCAGCC
    AATCAGCTTC GACATCAGCT TGAGGATCAA GACACAGAAA TTGAAAGACT TAAATCTGAA
    ATTATAGCTC TAAATAAAAC AAAAGAAAGA ATGCGACCTT ATCAAGGCAA CCAGGAAGAT
    GAGGATCCAG ATATTAAAAA AATTAAAAAG GTTCAGAGCT TTATGCGAGG CTGGTTATGT
    AGAAGAAAAT GGAAGACTAT TGTCCAAGAT TATATTTGTT CTCCCCATGC AGAAAGTATG
    AGAAAAAGGA ATCAGATAGT TTTTAACATG GTTGAGGCTG AATCTGAATA TGTGCATCAA
    CTTTATGTTC TTGTGAACTG TTTTCTGAGG CCCTTAAGAA TGGCTGCAAG TTCAAAGAAG
    CCACCTATCA GTCATGATGA TGTCAGTAGC ATTTTCCTCA ATAGTGAAAC AATCATGTTT
    CTTCATGAAA TATTTCATCA AGGTTTAAAA GCAAGAATTG CTAATTGGCC TACCTTAATT
    CTAGCTGACT TATTTGACAT TCTGCTGCCA ATGCTGAACA TATATCAAGA ATTTGTTCGG
    AATCATCAGT ATAGTCTACA AGTACTGGCA AACTGTAAGC AAAACAGAGA TTTTGACAAA
    CTCTTGAAGC AATATGAAGC CAACCCAGCA TGTGAGGGGC GAATGCTGGA AACTTTCCTT
    ACTTACCCCA TGTTTCAGGA TAATGAATTG AACTTAAGTT TCCTCAAACT GAAAATGCAC
    ACACACGTAT GCAGCCTCTG TGACCCAAAA GTTCAGTGTT CTGGCAGTTT TGTTTTGACT
    ATTCCTAGAT ACATAATAAC ACTTCATGAA CTCCTGGCTC ACACACCACA TGAACACGTT
    GAACGAAAAA GCCTTGAATT TGCAAAATCT AAACTAGAAG AACTTTCAAG GGTGATGCAT
    GATGAAGTGA GTGACACGGA AAACATCCGG AAGAATCTGG CCATAGAAAG AACAATAGTA
    GAAGGCTGTG ATATATTGCT AGATACTAGT CAAACTTTTA TACGCCAAGG TTCCCTTATT
    CAAGTCCCTT CAGTTGAAAG GGGAAAACTT AGCAAAGTTC GTCTGGGATC ATTGTCTTTG
    AAAAAAGAAG GAGAAAGGCA ATGCTTCTTA TTTACAAAAC ATTTTTTAAT TTGTACAAGA
    AGTTCAGGAG GAAAACTGCA TCTACTTAAG ACAGGAGGTG TTCTGTCGTT GATAGAGTGC
    ACATTGATTG AGGAGACAGA TGCAACTGAT GATGACTCAA AAAGCACTGG ACAGGTGTTT
    GGACACCTTG ATTTCAAGCT TGTAGTTGAA CCTACAGATG CTCCTCCTTT TACTGTTGTC
    CTCTTGGCTC CTTCACGTCA AGAGAAAGCT GCATGGACAA GTGATATAAG TCAGTGCATT
    GATAACATAA GGTGCAATGG TTTAATGACC ATAGTGTTTG AAGAAAATTC TAAAGTCACT
    GTGCCACATA TGATAAAGTC TGATGCACGT CTTCATAGAG ATGACATTGA TATCTGCTTC
    AGCAAAACTC TAAATTCCTG CAAAGTACCT CAAATCCGTT ATGCAAGTGT TGAACGCCTT
    TTGGAGAGGC TAACAGACTT GCGGTTTCTG AGCATTGATT TCCTGAATAC CTTCCTGCAT
    ACTTACCGCA TATTTACTAC TGCAGCTGTT GTACTGGAAA AACTTTCAGA CATATACAGA
    CGGCCTTTCA CTTCCATTCC AGTTAGGGCT TTGGAGTTAT TTTTTGCTAC CAGTCAGAAC
    AACAGAGGAG AGTACCTTGC TGATGCTAAG TCACCGCATC TCTGTCGCAA ATTTTCTTCT
    CCCCCTCCAC TGTCACTTTC CAGAACATCC TCACCTGTCA GAACTCGAAA ATTGTCATTG
    ACTTCACCAC TGAATTCAAG AATTGGTGCC CTTGATCTTT CTACTTCATC TGTGGCAAGC
    AGTCCTACCT CAACCTATAG TCCAGCCACC TCTCCTCCAC CAACAGGTAG CAAAGTACCT
    CTGGACCTTA GCAAAGGGCC CTCCTCTCCT GAGCAAAGCC CTGGCTCCAT AGAGGACAGC
    TTGGAGAACC CTCGCATTGA CCTGTGTAAC AAACTGAGGA GAAGCATTCG AAGAGCAGCA
    GTTCTGGAAT CAGTGTCAGT GGACAGAACT ATTCCTGAAA GCACCTCGGC AGTGGATACC
    ACAGAACTTT CCCCGTGTAG ATCCCCTTCA ACACCACGTC ATCTCCGCTA TCGTCAATCA
    GGAGTGCAAA CTGCTGACAA CAGTCACTGT GCTGTTTCTC CTGCATCAGC TTTTGCTATT
    GCTACAGCTG CAGCAGGGCA TGGGAGTCCG CCAGGTTTTA ACAATTCTGA ACGAATGTGT
    GACAAAGAGT TCATAATACG CAGAGCAGCC ACTAATCGTG TCCTGAATGT CTTACGTCAC
    TGGGTCTCCA AGCACTCTCA GGTAGCATGT ATAGGTTTGT GGAAGTTGGA GATCTTCGAA
    GATCTCAGCA GGTTCTTCAT GATTACTCCT TGTACAATTC CATTGCCTAA CCAATTGAGT
    GGCATTCTGC TGTGGAGTGA TGGCATCAGT GGACAAAGAA GGGGTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.