RASGRF2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF2B
Entrez Gene ID	553520
Full Name	Ras protein-specific guanine nucleotide-releasing factor 2b
Synonyms	rasgrf2,rasgrf2-2
General protein information	Preferred Names Ras protein-specific guanine nucleotide-releasing factor 2b Names ras-specific guanine nucleotide-releasing factor 2 Ras protein-specific guanine nucleotide-releasing factor 2 guanine nucleotide-releasing factor 2 ras guanine nucleotide exchange factor 2 ras-GRF2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301774.3	XP_009300049.1	ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_009301773.2	XP_009300048.1	ras-specific guanine nucleotide-releasing factor 2 isoform X3
XM_009301772.2	XP_009300047.1	ras-specific guanine nucleotide-releasing factor 2 isoform X2
XM_009301771.3	XP_009300046.1	ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_009301771.2	XP_009300046.1	ras-specific guanine nucleotide-releasing factor 2 isoform X1
XM_009301774.2	XP_009300049.1	ras-specific guanine nucleotide-releasing factor 2 isoform X4
NM_001128233.1	NP_001121705.1	ras-specific guanine nucleotide-releasing factor 2
XM_009301775.2	XP_009300050.1	ras-specific guanine nucleotide-releasing factor 2 isoform X5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RASGRF2	XP_005618219.1
Danio rerio (zebrafish)	rasgrf2b	NP_001121705.1
Pan troglodytes (chimpanzee)	RASGRF2	XP_517672.2
Xenopus tropicalis (tropical clawed frog)	rasgrf2	XP_002942408.2
Homo sapiens (human)	RASGRF2	NP_008840.1
Mus musculus (house mouse)	Rasgrf2	NP_033053.2
Rattus norvegicus (Norway rat)	Rasgrf2	NP_446173.1
Gallus gallus (chicken)	RASGRF2	XP_004949517.1
Bos taurus (cattle)	RASGRF2	XP_002689419.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001238	XP_321917.5

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RASGRF2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa41974	XM_009301775.2 Latest version!	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa38777	NM_001128233.1 Latest version!	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41970	XM_009301771.3 Latest version!	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41971	XM_009301772.2 Latest version!	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41972	XM_009301773.2 Latest version!	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41973	XM_009301774.3 Latest version!	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa41970	XM_009301771.2	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa41973	XM_009301774.2	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF2B ( XM_009301775.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300050.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa41974
Clone ID Related Accession (Same CDS sequence)	XM_009301775.2
Accession Version	XM_009301775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGAACCTA ATAAAGCTGA GCAAATCATG ACTATTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300050.1
CDS	377..2998
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRREPNKAEQIMTIG

Target ORF information:

RefSeq Version	XM_009301775.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301775.2

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGAACCTA ATAAAGCTGA GCAAATCATG ACTATTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( NM_001128233.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001121705.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), mRNA.

Price & Availability↑

CloneID	ODa38777
Clone ID Related Accession (Same CDS sequence)	NM_001128233.1
Accession Version	NM_001128233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR388055.6. On Jul 31, 2008 this sequence version replaced XM_001335759.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168447, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGATCCT TGGAGCTTTT CTTTGCCACC 
AATCAGAACA ACCGCAGCGG GGACCACGTC AATGACAAAT CTCCAAGACT GTGCCGCAAG 
TTCTCATCCC CTCCACCTCT CTCCATTTCC TCTCGTACTT CCTCTCCAGT TCGCACTAGA 
AAACTCTCTC TTAATTCACC TATTGGCTCA AAAGTAGGCA TCCTTGATCT GTCCACCACT 
TCATCATCTG CAGCAAGCAG TCCAACCTCC GCCAACCCCA CCATCTCACC TCCACCATCC 
AACAACAATA ACAACAGCAA GCCACCTTTG GATCTTAGCC GTGGACAGAG TCCTTCCTCC 
CCCGAGCAGA GTCCTGGAGC TCTGGACGAC AATGCAGAGG TGCCAAGAAT CGATGCCCTT 
TGTGGTAAAC TACGCCGCAG CATAAGGAGA GCTGTGCTGG AGTCAGTGTC TTTGGACAAA 
TTTATTCCAG AGTCGCCCCA GGCCAGTGAA CCAGGAGAAA TCTCTCCGTG CCGCTCACCC 
TCCACTCCAC GCCACCTCCG CTACAGACAG TCAGGAGTCC AGACGGCTGA GAACTCACGC 
TGCTCTGTTT CTCCAGCCTC TGCGTTTGCC ATCGCCACTG CAGCAGCTGG ACACAGCAGC 
CCTCCAGTTT TTAACAACTC TGAGAGGACT TGTGACAAGG AGTTCATTAT ACGTCGAGCG 
GCTACTAACA GAGTCCTGAA TGTCTTGCGA CACTGGGTCT CCAAACACTC ACAGGACTTT 
GAAATGAATG GAGAGTTGAA GATGGGTGTG ATCTGCCTGC TGGAAGAGGT GTTGAGAGAC 
CCAGACCTTC TCCCTCAGGA GAGAAAAGCC ACAGCAAACA TACTAAGTGC CCTTTCTCAA 
GATGACCAGG ATGATGCACA GCTGAAGATT GAAGACATTC TTCAGATGGC TGAGTGCCCA 
AAGGCCGAGT GTTTTGAGTC TCTGTCTGCA ATGGAGATAG CAGAACAAAT CACTCTCCTC 
GATCACATCG TGTTCAGGAG TATTCCCTAC GAGGAGTTCC TGGGCCAAGG ATGGATGAAG 
ACAGATAAGA CAGAGAGAAC GCCATACATC ATGAAGACAA GTCAACACTT CAATGACATG 
AGTAATCTGG TGGCCTCTCA GATCATGAGC CATACAGATG TGGGCTCCAG AGCGGGTTCT 
ATTGAAAAAT GGGTCGCAGT GGCTGACATT TGCCGTTGTC TCAACAATTA TAACGGTGTC 
TTGGAGATCA CATCTGCCCT GAACCGCAGC GCCATCTACA GGCTGAAGAA GACATGGGCT 
AAAGTCTGCA AACAGACTAA AGCACTAATG GACAAACTTC AGAAGACCGT GTCATCTGAG 
GGAAGATTTA AAAACTTGAG GGAAACTCTC AAAAACTGTA ATCCACCCTG TGTTCCATAT 
CTGGGCATGT ACTTGACAGA CCTTGCGTTT ATCGAAGAGG GAACTCCAAA TTTCACAGAG 
GAAGGTCTAG TTAACTTCTC AAAGATGAGA ATGATTTCTC ACATCATACG AGAGATCCGA 
CAGTTCCAGC AAACTCCCTA CAGAATAGAG CACCAACCAA AGGTCACGCA GTATCTTCTG 
GATAAGACCT TGATTATGGA TGAGGACACA CTTTATGATT TATCACTCAA GATTGAACCC 
AGGCTTCCTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121705.1
CDS	385..4119
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRAVLESVSLDKFIPESPQASEPGEISPCRSPSTPRHLRYRQSGVQTAENSRCSVSPASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	NM_001128233.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128233.1

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGATCCT TGGAGCTTTT CTTTGCCACC 
AATCAGAACA ACCGCAGCGG GGACCACGTC AATGACAAAT CTCCAAGACT GTGCCGCAAG 
TTCTCATCCC CTCCACCTCT CTCCATTTCC TCTCGTACTT CCTCTCCAGT TCGCACTAGA 
AAACTCTCTC TTAATTCACC TATTGGCTCA AAAGTAGGCA TCCTTGATCT GTCCACCACT 
TCATCATCTG CAGCAAGCAG TCCAACCTCC GCCAACCCCA CCATCTCACC TCCACCATCC 
AACAACAATA ACAACAGCAA GCCACCTTTG GATCTTAGCC GTGGACAGAG TCCTTCCTCC 
CCCGAGCAGA GTCCTGGAGC TCTGGACGAC AATGCAGAGG TGCCAAGAAT CGATGCCCTT 
TGTGGTAAAC TACGCCGCAG CATAAGGAGA GCTGTGCTGG AGTCAGTGTC TTTGGACAAA 
TTTATTCCAG AGTCGCCCCA GGCCAGTGAA CCAGGAGAAA TCTCTCCGTG CCGCTCACCC 
TCCACTCCAC GCCACCTCCG CTACAGACAG TCAGGAGTCC AGACGGCTGA GAACTCACGC 
TGCTCTGTTT CTCCAGCCTC TGCGTTTGCC ATCGCCACTG CAGCAGCTGG ACACAGCAGC 
CCTCCAGTTT TTAACAACTC TGAGAGGACT TGTGACAAGG AGTTCATTAT ACGTCGAGCG 
GCTACTAACA GAGTCCTGAA TGTCTTGCGA CACTGGGTCT CCAAACACTC ACAGGACTTT 
GAAATGAATG GAGAGTTGAA GATGGGTGTG ATCTGCCTGC TGGAAGAGGT GTTGAGAGAC 
CCAGACCTTC TCCCTCAGGA GAGAAAAGCC ACAGCAAACA TACTAAGTGC CCTTTCTCAA 
GATGACCAGG ATGATGCACA GCTGAAGATT GAAGACATTC TTCAGATGGC TGAGTGCCCA 
AAGGCCGAGT GTTTTGAGTC TCTGTCTGCA ATGGAGATAG CAGAACAAAT CACTCTCCTC 
GATCACATCG TGTTCAGGAG TATTCCCTAC GAGGAGTTCC TGGGCCAAGG ATGGATGAAG 
ACAGATAAGA CAGAGAGAAC GCCATACATC ATGAAGACAA GTCAACACTT CAATGACATG 
AGTAATCTGG TGGCCTCTCA GATCATGAGC CATACAGATG TGGGCTCCAG AGCGGGTTCT 
ATTGAAAAAT GGGTCGCAGT GGCTGACATT TGCCGTTGTC TCAACAATTA TAACGGTGTC 
TTGGAGATCA CATCTGCCCT GAACCGCAGC GCCATCTACA GGCTGAAGAA GACATGGGCT 
AAAGTCTGCA AACAGACTAA AGCACTAATG GACAAACTTC AGAAGACCGT GTCATCTGAG 
GGAAGATTTA AAAACTTGAG GGAAACTCTC AAAAACTGTA ATCCACCCTG TGTTCCATAT 
CTGGGCATGT ACTTGACAGA CCTTGCGTTT ATCGAAGAGG GAACTCCAAA TTTCACAGAG 
GAAGGTCTAG TTAACTTCTC AAAGATGAGA ATGATTTCTC ACATCATACG AGAGATCCGA 
CAGTTCCAGC AAACTCCCTA CAGAATAGAG CACCAACCAA AGGTCACGCA GTATCTTCTG 
GATAAGACCT TGATTATGGA TGAGGACACA CTTTATGATT TATCACTCAA GATTGAACCC 
AGGCTTCCTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( XM_009301771.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300046.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X1, mRNA.

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CloneID	ODa41970
Clone ID Related Accession (Same CDS sequence)	XM_009301771.3 , XM_009301771.2
Accession Version	XM_009301771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGCTGTGC TGGAGTCAGT GTCTTTGGAC AAATTTATTC CAGAGTCGCC CCAGGCCAGT 
GAACCAGGAG AAATCTCTCC GTGCCGCTCA CCCTCCACTC CACGCCACCT CCGCTACAGA 
CAGTCAGGAG TCCAGACGGC TGAGAACTCA CGCTGCTCTG TTTCTCCAGC CTCTGCGTTT 
GCCATCGCCA CTGCAGCAGC TGGACACAGC AGCCCTCCAG TTTTTAACAA CTCTGAGAGG 
ACTTGTGACA AGGAGTTCAT TATACGTCGA GCGGCTACTA ACAGAGTCCT GAATGTCTTG 
CGACACTGGG TCTCCAAACA CTCACAGGAC TTTGAAATGA ATGGAGAGTT GAAGATGGGT 
GTGATCTGCC TGCTGGAAGA GGTGTTGAGA GACCCAGACC TTCTCCCTCA GGAGAGAAAA 
GCCACAGCAA ACATACTAAG TGCCCTTTCT CAAGATGACC AGGATGATGC ACAGCTGAAG 
ATTGAAGACA TTCTTCAGAT GGCTGAGTGC CCAAAGGCCG AGTGTTTTGA GTCTCTGTCT 
GCAATGGAGA TAGCAGAACA AATCACTCTC CTCGATCACA TCGTGTTCAG GAGTATTCCC 
TACGAGGAGT TCCTGGGCCA AGGATGGATG AAGACAGATA AGACAGAGAG AACGCCATAC 
ATCATGAAGA CAAGTCAACA CTTCAATGAC ATGAGTAATC TGGTGGCCTC TCAGATCATG 
AGCCATACAG ATGTGGGCTC CAGAGCGGGT TCTATTGAAA AATGGGTCGC AGTGGCTGAC 
ATTTGCCGTT GTCTCAACAA TTATAACGGT GTCTTGGAGA TCACATCTGC CCTGAACCGC 
AGCGCCATCT ACAGGCTGAA GAAGACATGG GCTAAAGTCT GCAAACAGAC TAAAGCACTA 
ATGGACAAAC TTCAGAAGAC CGTGTCATCT GAGGGAAGAT TTAAAAACTT GAGGGAAACT 
CTCAAAAACT GTAATCCACC CTGTGTTCCA TATCTGGGCA TGTACTTGAC AGACCTTGCG 
TTTATCGAAG AGGGAACTCC AAATTTCACA GAGGAAGGTC TAGTTAACTT CTCAAAGATG 
AGAATGATTT CTCACATCAT ACGAGAGATC CGACAGTTCC AGCAAACTCC CTACAGAATA 
GAGCACCAAC CAAAGGTCAC GCAGTATCTT CTGGATAAGA CCTTGATTAT GGATGAGGAC 
ACACTTTATG ATTTATCACT CAAGATTGAA CCCAGGCTTC CTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300046.1
CDS	372..4199
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRAVLESVSLDKFIPESPQASEPGEISPCRSPSTPRHLRYRQSGVQTAENSRCSVSPASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_009301771.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301771.3

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGCTGTGC TGGAGTCAGT GTCTTTGGAC AAATTTATTC CAGAGTCGCC CCAGGCCAGT 
GAACCAGGAG AAATCTCTCC GTGCCGCTCA CCCTCCACTC CACGCCACCT CCGCTACAGA 
CAGTCAGGAG TCCAGACGGC TGAGAACTCA CGCTGCTCTG TTTCTCCAGC CTCTGCGTTT 
GCCATCGCCA CTGCAGCAGC TGGACACAGC AGCCCTCCAG TTTTTAACAA CTCTGAGAGG 
ACTTGTGACA AGGAGTTCAT TATACGTCGA GCGGCTACTA ACAGAGTCCT GAATGTCTTG 
CGACACTGGG TCTCCAAACA CTCACAGGAC TTTGAAATGA ATGGAGAGTT GAAGATGGGT 
GTGATCTGCC TGCTGGAAGA GGTGTTGAGA GACCCAGACC TTCTCCCTCA GGAGAGAAAA 
GCCACAGCAA ACATACTAAG TGCCCTTTCT CAAGATGACC AGGATGATGC ACAGCTGAAG 
ATTGAAGACA TTCTTCAGAT GGCTGAGTGC CCAAAGGCCG AGTGTTTTGA GTCTCTGTCT 
GCAATGGAGA TAGCAGAACA AATCACTCTC CTCGATCACA TCGTGTTCAG GAGTATTCCC 
TACGAGGAGT TCCTGGGCCA AGGATGGATG AAGACAGATA AGACAGAGAG AACGCCATAC 
ATCATGAAGA CAAGTCAACA CTTCAATGAC ATGAGTAATC TGGTGGCCTC TCAGATCATG 
AGCCATACAG ATGTGGGCTC CAGAGCGGGT TCTATTGAAA AATGGGTCGC AGTGGCTGAC 
ATTTGCCGTT GTCTCAACAA TTATAACGGT GTCTTGGAGA TCACATCTGC CCTGAACCGC 
AGCGCCATCT ACAGGCTGAA GAAGACATGG GCTAAAGTCT GCAAACAGAC TAAAGCACTA 
ATGGACAAAC TTCAGAAGAC CGTGTCATCT GAGGGAAGAT TTAAAAACTT GAGGGAAACT 
CTCAAAAACT GTAATCCACC CTGTGTTCCA TATCTGGGCA TGTACTTGAC AGACCTTGCG 
TTTATCGAAG AGGGAACTCC AAATTTCACA GAGGAAGGTC TAGTTAACTT CTCAAAGATG 
AGAATGATTT CTCACATCAT ACGAGAGATC CGACAGTTCC AGCAAACTCC CTACAGAATA 
GAGCACCAAC CAAAGGTCAC GCAGTATCTT CTGGATAAGA CCTTGATTAT GGATGAGGAC 
ACACTTTATG ATTTATCACT CAAGATTGAA CCCAGGCTTC CTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( XM_009301771.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300046.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41970
Clone ID Related Accession (Same CDS sequence)	XM_009301771.3 , XM_009301771.2
Accession Version	XM_009301771.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGCTGTGC TGGAGTCAGT GTCTTTGGAC AAATTTATTC CAGAGTCGCC CCAGGCCAGT 
GAACCAGGAG AAATCTCTCC GTGCCGCTCA CCCTCCACTC CACGCCACCT CCGCTACAGA 
CAGTCAGGAG TCCAGACGGC TGAGAACTCA CGCTGCTCTG TTTCTCCAGC CTCTGCGTTT 
GCCATCGCCA CTGCAGCAGC TGGACACAGC AGCCCTCCAG TTTTTAACAA CTCTGAGAGG 
ACTTGTGACA AGGAGTTCAT TATACGTCGA GCGGCTACTA ACAGAGTCCT GAATGTCTTG 
CGACACTGGG TCTCCAAACA CTCACAGGAC TTTGAAATGA ATGGAGAGTT GAAGATGGGT 
GTGATCTGCC TGCTGGAAGA GGTGTTGAGA GACCCAGACC TTCTCCCTCA GGAGAGAAAA 
GCCACAGCAA ACATACTAAG TGCCCTTTCT CAAGATGACC AGGATGATGC ACAGCTGAAG 
ATTGAAGACA TTCTTCAGAT GGCTGAGTGC CCAAAGGCCG AGTGTTTTGA GTCTCTGTCT 
GCAATGGAGA TAGCAGAACA AATCACTCTC CTCGATCACA TCGTGTTCAG GAGTATTCCC 
TACGAGGAGT TCCTGGGCCA AGGATGGATG AAGACAGATA AGACAGAGAG AACGCCATAC 
ATCATGAAGA CAAGTCAACA CTTCAATGAC ATGAGTAATC TGGTGGCCTC TCAGATCATG 
AGCCATACAG ATGTGGGCTC CAGAGCGGGT TCTATTGAAA AATGGGTCGC AGTGGCTGAC 
ATTTGCCGTT GTCTCAACAA TTATAACGGT GTCTTGGAGA TCACATCTGC CCTGAACCGC 
AGCGCCATCT ACAGGCTGAA GAAGACATGG GCTAAAGTCT GCAAACAGAC TAAAGCACTA 
ATGGACAAAC TTCAGAAGAC CGTGTCATCT GAGGGAAGAT TTAAAAACTT GAGGGAAACT 
CTCAAAAACT GTAATCCACC CTGTGTTCCA TATCTGGGCA TGTACTTGAC AGACCTTGCG 
TTTATCGAAG AGGGAACTCC AAATTTCACA GAGGAAGGTC TAGTTAACTT CTCAAAGATG 
AGAATGATTT CTCACATCAT ACGAGAGATC CGACAGTTCC AGCAAACTCC CTACAGAATA 
GAGCACCAAC CAAAGGTCAC GCAGTATCTT CTGGATAAGA CCTTGATTAT GGATGAGGAC 
ACACTTTATG ATTTATCACT CAAGATTGAA CCCAGGCTTC CTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300046.1
CDS	377..4204
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRAVLESVSLDKFIPESPQASEPGEISPCRSPSTPRHLRYRQSGVQTAENSRCSVSPASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_009301771.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301771.2

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGCTGTGC TGGAGTCAGT GTCTTTGGAC AAATTTATTC CAGAGTCGCC CCAGGCCAGT 
GAACCAGGAG AAATCTCTCC GTGCCGCTCA CCCTCCACTC CACGCCACCT CCGCTACAGA 
CAGTCAGGAG TCCAGACGGC TGAGAACTCA CGCTGCTCTG TTTCTCCAGC CTCTGCGTTT 
GCCATCGCCA CTGCAGCAGC TGGACACAGC AGCCCTCCAG TTTTTAACAA CTCTGAGAGG 
ACTTGTGACA AGGAGTTCAT TATACGTCGA GCGGCTACTA ACAGAGTCCT GAATGTCTTG 
CGACACTGGG TCTCCAAACA CTCACAGGAC TTTGAAATGA ATGGAGAGTT GAAGATGGGT 
GTGATCTGCC TGCTGGAAGA GGTGTTGAGA GACCCAGACC TTCTCCCTCA GGAGAGAAAA 
GCCACAGCAA ACATACTAAG TGCCCTTTCT CAAGATGACC AGGATGATGC ACAGCTGAAG 
ATTGAAGACA TTCTTCAGAT GGCTGAGTGC CCAAAGGCCG AGTGTTTTGA GTCTCTGTCT 
GCAATGGAGA TAGCAGAACA AATCACTCTC CTCGATCACA TCGTGTTCAG GAGTATTCCC 
TACGAGGAGT TCCTGGGCCA AGGATGGATG AAGACAGATA AGACAGAGAG AACGCCATAC 
ATCATGAAGA CAAGTCAACA CTTCAATGAC ATGAGTAATC TGGTGGCCTC TCAGATCATG 
AGCCATACAG ATGTGGGCTC CAGAGCGGGT TCTATTGAAA AATGGGTCGC AGTGGCTGAC 
ATTTGCCGTT GTCTCAACAA TTATAACGGT GTCTTGGAGA TCACATCTGC CCTGAACCGC 
AGCGCCATCT ACAGGCTGAA GAAGACATGG GCTAAAGTCT GCAAACAGAC TAAAGCACTA 
ATGGACAAAC TTCAGAAGAC CGTGTCATCT GAGGGAAGAT TTAAAAACTT GAGGGAAACT 
CTCAAAAACT GTAATCCACC CTGTGTTCCA TATCTGGGCA TGTACTTGAC AGACCTTGCG 
TTTATCGAAG AGGGAACTCC AAATTTCACA GAGGAAGGTC TAGTTAACTT CTCAAAGATG 
AGAATGATTT CTCACATCAT ACGAGAGATC CGACAGTTCC AGCAAACTCC CTACAGAATA 
GAGCACCAAC CAAAGGTCAC GCAGTATCTT CTGGATAAGA CCTTGATTAT GGATGAGGAC 
ACACTTTATG ATTTATCACT CAAGATTGAA CCCAGGCTTC CTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( XM_009301772.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300047.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa41971
Clone ID Related Accession (Same CDS sequence)	XM_009301772.2
Accession Version	XM_009301772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGCTGTGC TGGAGTCAGT GTCTTTGGAC AAATTTATTC CAGAGTCGCC CCAGGCCAGT 
GAACCAGGAG AAATCTCTCC GTGCCGCTCA CCCTCCACTC CACGCCACCT CCGCTACAGA 
CAGTCAGGAG CCTCTGCGTT TGCCATCGCC ACTGCAGCAG CTGGACACAG CAGCCCTCCA 
GTTTTTAACA ACTCTGAGAG GACTTGTGAC AAGGAGTTCA TTATACGTCG AGCGGCTACT 
AACAGAGTCC TGAATGTCTT GCGACACTGG GTCTCCAAAC ACTCACAGGA CTTTGAAATG 
AATGGAGAGT TGAAGATGGG TGTGATCTGC CTGCTGGAAG AGGTGTTGAG AGACCCAGAC 
CTTCTCCCTC AGGAGAGAAA AGCCACAGCA AACATACTAA GTGCCCTTTC TCAAGATGAC 
CAGGATGATG CACAGCTGAA GATTGAAGAC ATTCTTCAGA TGGCTGAGTG CCCAAAGGCC 
GAGTGTTTTG AGTCTCTGTC TGCAATGGAG ATAGCAGAAC AAATCACTCT CCTCGATCAC 
ATCGTGTTCA GGAGTATTCC CTACGAGGAG TTCCTGGGCC AAGGATGGAT GAAGACAGAT 
AAGACAGAGA GAACGCCATA CATCATGAAG ACAAGTCAAC ACTTCAATGA CATGAGTAAT 
CTGGTGGCCT CTCAGATCAT GAGCCATACA GATGTGGGCT CCAGAGCGGG TTCTATTGAA 
AAATGGGTCG CAGTGGCTGA CATTTGCCGT TGTCTCAACA ATTATAACGG TGTCTTGGAG 
ATCACATCTG CCCTGAACCG CAGCGCCATC TACAGGCTGA AGAAGACATG GGCTAAAGTC 
TGCAAACAGA CTAAAGCACT AATGGACAAA CTTCAGAAGA CCGTGTCATC TGAGGGAAGA 
TTTAAAAACT TGAGGGAAAC TCTCAAAAAC TGTAATCCAC CCTGTGTTCC ATATCTGGGC 
ATGTACTTGA CAGACCTTGC GTTTATCGAA GAGGGAACTC CAAATTTCAC AGAGGAAGGT 
CTAGTTAACT TCTCAAAGAT GAGAATGATT TCTCACATCA TACGAGAGAT CCGACAGTTC 
CAGCAAACTC CCTACAGAAT AGAGCACCAA CCAAAGGTCA CGCAGTATCT TCTGGATAAG 
ACCTTGATTA TGGATGAGGA CACACTTTAT GATTTATCAC TCAAGATTGA ACCCAGGCTT 
CCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300047.1
CDS	377..4165
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRAVLESVSLDKFIPESPQASEPGEISPCRSPSTPRHLRYRQSGASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_009301772.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301772.2

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGCTGTGC TGGAGTCAGT GTCTTTGGAC AAATTTATTC CAGAGTCGCC CCAGGCCAGT 
GAACCAGGAG AAATCTCTCC GTGCCGCTCA CCCTCCACTC CACGCCACCT CCGCTACAGA 
CAGTCAGGAG CCTCTGCGTT TGCCATCGCC ACTGCAGCAG CTGGACACAG CAGCCCTCCA 
GTTTTTAACA ACTCTGAGAG GACTTGTGAC AAGGAGTTCA TTATACGTCG AGCGGCTACT 
AACAGAGTCC TGAATGTCTT GCGACACTGG GTCTCCAAAC ACTCACAGGA CTTTGAAATG 
AATGGAGAGT TGAAGATGGG TGTGATCTGC CTGCTGGAAG AGGTGTTGAG AGACCCAGAC 
CTTCTCCCTC AGGAGAGAAA AGCCACAGCA AACATACTAA GTGCCCTTTC TCAAGATGAC 
CAGGATGATG CACAGCTGAA GATTGAAGAC ATTCTTCAGA TGGCTGAGTG CCCAAAGGCC 
GAGTGTTTTG AGTCTCTGTC TGCAATGGAG ATAGCAGAAC AAATCACTCT CCTCGATCAC 
ATCGTGTTCA GGAGTATTCC CTACGAGGAG TTCCTGGGCC AAGGATGGAT GAAGACAGAT 
AAGACAGAGA GAACGCCATA CATCATGAAG ACAAGTCAAC ACTTCAATGA CATGAGTAAT 
CTGGTGGCCT CTCAGATCAT GAGCCATACA GATGTGGGCT CCAGAGCGGG TTCTATTGAA 
AAATGGGTCG CAGTGGCTGA CATTTGCCGT TGTCTCAACA ATTATAACGG TGTCTTGGAG 
ATCACATCTG CCCTGAACCG CAGCGCCATC TACAGGCTGA AGAAGACATG GGCTAAAGTC 
TGCAAACAGA CTAAAGCACT AATGGACAAA CTTCAGAAGA CCGTGTCATC TGAGGGAAGA 
TTTAAAAACT TGAGGGAAAC TCTCAAAAAC TGTAATCCAC CCTGTGTTCC ATATCTGGGC 
ATGTACTTGA CAGACCTTGC GTTTATCGAA GAGGGAACTC CAAATTTCAC AGAGGAAGGT 
CTAGTTAACT TCTCAAAGAT GAGAATGATT TCTCACATCA TACGAGAGAT CCGACAGTTC 
CAGCAAACTC CCTACAGAAT AGAGCACCAA CCAAAGGTCA CGCAGTATCT TCTGGATAAG 
ACCTTGATTA TGGATGAGGA CACACTTTAT GATTTATCAC TCAAGATTGA ACCCAGGCTT 
CCTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( XM_009301773.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300048.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X3, mRNA.

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CloneID	ODa41972
Clone ID Related Accession (Same CDS sequence)	XM_009301773.2
Accession Version	XM_009301773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAATAAG ACCCTCAACT CCTGCAAGGT TCCTCAGATC 
CGCTACGCCA GCGTGGAGAG ACTGCTGGAG AGGTTGACCG ACCTGCGCTT CCTCTCCATA 
GACTTCCTCA ACACATTCCT GCACACCTAC CGCATCTTCA CTACTGCCAC CGTGGTCATG 
GAGAAACTGG CTGACATCTA CAAGAAGCCT TTTACTTCCA TACCAGTCAG GGTCCAGTAT 
CACTCATTTG ACCGCTTGTC CATTACATCC ATCTGCCCAG ACTACAGCTT GAAGATCAGG 
AAGATAGCGC TGGATCAGTC AAAATCCTTG GAGCTTTTCT TTGCCACCAA TCAGAACAAC 
CGCAGCGGGG ACCACGTCAA TGACAAATCT CCAAGACTGT GCCGCAAGTT CTCATCCCCT 
CCACCTCTCT CCATTTCCTC TCGTACTTCC TCTCCAGTTC GCACTAGAAA ACTCTCTCTT 
AATTCACCTA TTGGCTCAAA AGTAGGCATC CTTGATCTGT CCACCACTTC ATCATCTGCA 
GCAAGCAGTC CAACCTCCGC CAACCCCACC ATCTCACCTC CACCATCCAA CAACAATAAC 
AACAGCAAGC CACCTTTGGA TCTTAGCCGT GGACAGAGTC CTTCCTCCCC CGAGCAGAGT 
CCTGGAGCTC TGGACGACAA TGCAGAGGTG CCAAGAATCG ATGCCCTTTG TGGTAAACTA 
CGCCGCAGCA TAAGGAGAGC TGTGCTGGAG TCAGTGTCTT TGGACAAATT TATTCCAGAG 
TCGCCCCAGG CCAGTGAACC AGGAGAAATC TCTCCGTGCC GCTCACCCTC CACTCCACGC 
CACCTCCGCT ACAGACAGTC AGGAGTCCAG ACGGCTGAGA ACTCACGCTG CTCTGTTTCT 
CCAGCCTCTG CGTTTGCCAT CGCCACTGCA GCAGCTGGAC ACAGCAGCCC TCCAGTTTTT 
AACAACTCTG AGAGGACTTG TGACAAGGAG TTCATTATAC GTCGAGCGGC TACTAACAGA 
GTCCTGAATG TCTTGCGACA CTGGGTCTCC AAACACTCAC AGGACTTTGA AATGAATGGA 
GAGTTGAAGA TGGGTGTGAT CTGCCTGCTG GAAGAGGTGT TGAGAGACCC AGACCTTCTC 
CCTCAGGAGA GAAAAGCCAC AGCAAACATA CTAAGTGCCC TTTCTCAAGA TGACCAGGAT 
GATGCACAGC TGAAGATTGA AGACATTCTT CAGATGGCTG AGTGCCCAAA GGCCGAGTGT 
TTTGAGTCTC TGTCTGCAAT GGAGATAGCA GAACAAATCA CTCTCCTCGA TCACATCGTG 
TTCAGGAGTA TTCCCTACGA GGAGTTCCTG GGCCAAGGAT GGATGAAGAC AGATAAGACA 
GAGAGAACGC CATACATCAT GAAGACAAGT CAACACTTCA ATGACATGAG TAATCTGGTG 
GCCTCTCAGA TCATGAGCCA TACAGATGTG GGCTCCAGAG CGGGTTCTAT TGAAAAATGG 
GTCGCAGTGG CTGACATTTG CCGTTGTCTC AACAATTATA ACGGTGTCTT GGAGATCACA 
TCTGCCCTGA ACCGCAGCGC CATCTACAGG CTGAAGAAGA CATGGGCTAA AGTCTGCAAA 
CAGACTAAAG CACTAATGGA CAAACTTCAG AAGACCGTGT CATCTGAGGG AAGATTTAAA 
AACTTGAGGG AAACTCTCAA AAACTGTAAT CCACCCTGTG TTCCATATCT GGGCATGTAC 
TTGACAGACC TTGCGTTTAT CGAAGAGGGA ACTCCAAATT TCACAGAGGA AGGTCTAGTT 
AACTTCTCAA AGATGAGAAT GATTTCTCAC ATCATACGAG AGATCCGACA GTTCCAGCAA 
ACTCCCTACA GAATAGAGCA CCAACCAAAG GTCACGCAGT ATCTTCTGGA TAAGACCTTG 
ATTATGGATG AGGACACACT TTATGATTTA TCACTCAAGA TTGAACCCAG GCTTCCTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300048.1
CDS	377..4159
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMINKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRAVLESVSLDKFIPESPQASEPGEISPCRSPSTPRHLRYRQSGVQTAENSRCSVSPASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_009301773.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301773.2

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAATAAG ACCCTCAACT CCTGCAAGGT TCCTCAGATC 
CGCTACGCCA GCGTGGAGAG ACTGCTGGAG AGGTTGACCG ACCTGCGCTT CCTCTCCATA 
GACTTCCTCA ACACATTCCT GCACACCTAC CGCATCTTCA CTACTGCCAC CGTGGTCATG 
GAGAAACTGG CTGACATCTA CAAGAAGCCT TTTACTTCCA TACCAGTCAG GGTCCAGTAT 
CACTCATTTG ACCGCTTGTC CATTACATCC ATCTGCCCAG ACTACAGCTT GAAGATCAGG 
AAGATAGCGC TGGATCAGTC AAAATCCTTG GAGCTTTTCT TTGCCACCAA TCAGAACAAC 
CGCAGCGGGG ACCACGTCAA TGACAAATCT CCAAGACTGT GCCGCAAGTT CTCATCCCCT 
CCACCTCTCT CCATTTCCTC TCGTACTTCC TCTCCAGTTC GCACTAGAAA ACTCTCTCTT 
AATTCACCTA TTGGCTCAAA AGTAGGCATC CTTGATCTGT CCACCACTTC ATCATCTGCA 
GCAAGCAGTC CAACCTCCGC CAACCCCACC ATCTCACCTC CACCATCCAA CAACAATAAC 
AACAGCAAGC CACCTTTGGA TCTTAGCCGT GGACAGAGTC CTTCCTCCCC CGAGCAGAGT 
CCTGGAGCTC TGGACGACAA TGCAGAGGTG CCAAGAATCG ATGCCCTTTG TGGTAAACTA 
CGCCGCAGCA TAAGGAGAGC TGTGCTGGAG TCAGTGTCTT TGGACAAATT TATTCCAGAG 
TCGCCCCAGG CCAGTGAACC AGGAGAAATC TCTCCGTGCC GCTCACCCTC CACTCCACGC 
CACCTCCGCT ACAGACAGTC AGGAGTCCAG ACGGCTGAGA ACTCACGCTG CTCTGTTTCT 
CCAGCCTCTG CGTTTGCCAT CGCCACTGCA GCAGCTGGAC ACAGCAGCCC TCCAGTTTTT 
AACAACTCTG AGAGGACTTG TGACAAGGAG TTCATTATAC GTCGAGCGGC TACTAACAGA 
GTCCTGAATG TCTTGCGACA CTGGGTCTCC AAACACTCAC AGGACTTTGA AATGAATGGA 
GAGTTGAAGA TGGGTGTGAT CTGCCTGCTG GAAGAGGTGT TGAGAGACCC AGACCTTCTC 
CCTCAGGAGA GAAAAGCCAC AGCAAACATA CTAAGTGCCC TTTCTCAAGA TGACCAGGAT 
GATGCACAGC TGAAGATTGA AGACATTCTT CAGATGGCTG AGTGCCCAAA GGCCGAGTGT 
TTTGAGTCTC TGTCTGCAAT GGAGATAGCA GAACAAATCA CTCTCCTCGA TCACATCGTG 
TTCAGGAGTA TTCCCTACGA GGAGTTCCTG GGCCAAGGAT GGATGAAGAC AGATAAGACA 
GAGAGAACGC CATACATCAT GAAGACAAGT CAACACTTCA ATGACATGAG TAATCTGGTG 
GCCTCTCAGA TCATGAGCCA TACAGATGTG GGCTCCAGAG CGGGTTCTAT TGAAAAATGG 
GTCGCAGTGG CTGACATTTG CCGTTGTCTC AACAATTATA ACGGTGTCTT GGAGATCACA 
TCTGCCCTGA ACCGCAGCGC CATCTACAGG CTGAAGAAGA CATGGGCTAA AGTCTGCAAA 
CAGACTAAAG CACTAATGGA CAAACTTCAG AAGACCGTGT CATCTGAGGG AAGATTTAAA 
AACTTGAGGG AAACTCTCAA AAACTGTAAT CCACCCTGTG TTCCATATCT GGGCATGTAC 
TTGACAGACC TTGCGTTTAT CGAAGAGGGA ACTCCAAATT TCACAGAGGA AGGTCTAGTT 
AACTTCTCAA AGATGAGAAT GATTTCTCAC ATCATACGAG AGATCCGACA GTTCCAGCAA 
ACTCCCTACA GAATAGAGCA CCAACCAAAG GTCACGCAGT ATCTTCTGGA TAAGACCTTG 
ATTATGGATG AGGACACACT TTATGATTTA TCACTCAAGA TTGAACCCAG GCTTCCTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( XM_009301774.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300049.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa41973
Clone ID Related Accession (Same CDS sequence)	XM_009301774.3 , XM_009301774.2
Accession Version	XM_009301774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGTCCAGA CGGCTGAGAA CTCACGCTGC TCTGTTTCTC CAGCCTCTGC GTTTGCCATC 
GCCACTGCAG CAGCTGGACA CAGCAGCCCT CCAGTTTTTA ACAACTCTGA GAGGACTTGT 
GACAAGGAGT TCATTATACG TCGAGCGGCT ACTAACAGAG TCCTGAATGT CTTGCGACAC 
TGGGTCTCCA AACACTCACA GGACTTTGAA ATGAATGGAG AGTTGAAGAT GGGTGTGATC 
TGCCTGCTGG AAGAGGTGTT GAGAGACCCA GACCTTCTCC CTCAGGAGAG AAAAGCCACA 
GCAAACATAC TAAGTGCCCT TTCTCAAGAT GACCAGGATG ATGCACAGCT GAAGATTGAA 
GACATTCTTC AGATGGCTGA GTGCCCAAAG GCCGAGTGTT TTGAGTCTCT GTCTGCAATG 
GAGATAGCAG AACAAATCAC TCTCCTCGAT CACATCGTGT TCAGGAGTAT TCCCTACGAG 
GAGTTCCTGG GCCAAGGATG GATGAAGACA GATAAGACAG AGAGAACGCC ATACATCATG 
AAGACAAGTC AACACTTCAA TGACATGAGT AATCTGGTGG CCTCTCAGAT CATGAGCCAT 
ACAGATGTGG GCTCCAGAGC GGGTTCTATT GAAAAATGGG TCGCAGTGGC TGACATTTGC 
CGTTGTCTCA ACAATTATAA CGGTGTCTTG GAGATCACAT CTGCCCTGAA CCGCAGCGCC 
ATCTACAGGC TGAAGAAGAC ATGGGCTAAA GTCTGCAAAC AGACTAAAGC ACTAATGGAC 
AAACTTCAGA AGACCGTGTC ATCTGAGGGA AGATTTAAAA ACTTGAGGGA AACTCTCAAA 
AACTGTAATC CACCCTGTGT TCCATATCTG GGCATGTACT TGACAGACCT TGCGTTTATC 
GAAGAGGGAA CTCCAAATTT CACAGAGGAA GGTCTAGTTA ACTTCTCAAA GATGAGAATG 
ATTTCTCACA TCATACGAGA GATCCGACAG TTCCAGCAAA CTCCCTACAG AATAGAGCAC 
CAACCAAAGG TCACGCAGTA TCTTCTGGAT AAGACCTTGA TTATGGATGA GGACACACTT 
TATGATTTAT CACTCAAGAT TGAACCCAGG CTTCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300049.1
CDS	372..4073
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRVQTAENSRCSVSPASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_009301774.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301774.3

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGTCCAGA CGGCTGAGAA CTCACGCTGC TCTGTTTCTC CAGCCTCTGC GTTTGCCATC 
GCCACTGCAG CAGCTGGACA CAGCAGCCCT CCAGTTTTTA ACAACTCTGA GAGGACTTGT 
GACAAGGAGT TCATTATACG TCGAGCGGCT ACTAACAGAG TCCTGAATGT CTTGCGACAC 
TGGGTCTCCA AACACTCACA GGACTTTGAA ATGAATGGAG AGTTGAAGAT GGGTGTGATC 
TGCCTGCTGG AAGAGGTGTT GAGAGACCCA GACCTTCTCC CTCAGGAGAG AAAAGCCACA 
GCAAACATAC TAAGTGCCCT TTCTCAAGAT GACCAGGATG ATGCACAGCT GAAGATTGAA 
GACATTCTTC AGATGGCTGA GTGCCCAAAG GCCGAGTGTT TTGAGTCTCT GTCTGCAATG 
GAGATAGCAG AACAAATCAC TCTCCTCGAT CACATCGTGT TCAGGAGTAT TCCCTACGAG 
GAGTTCCTGG GCCAAGGATG GATGAAGACA GATAAGACAG AGAGAACGCC ATACATCATG 
AAGACAAGTC AACACTTCAA TGACATGAGT AATCTGGTGG CCTCTCAGAT CATGAGCCAT 
ACAGATGTGG GCTCCAGAGC GGGTTCTATT GAAAAATGGG TCGCAGTGGC TGACATTTGC 
CGTTGTCTCA ACAATTATAA CGGTGTCTTG GAGATCACAT CTGCCCTGAA CCGCAGCGCC 
ATCTACAGGC TGAAGAAGAC ATGGGCTAAA GTCTGCAAAC AGACTAAAGC ACTAATGGAC 
AAACTTCAGA AGACCGTGTC ATCTGAGGGA AGATTTAAAA ACTTGAGGGA AACTCTCAAA 
AACTGTAATC CACCCTGTGT TCCATATCTG GGCATGTACT TGACAGACCT TGCGTTTATC 
GAAGAGGGAA CTCCAAATTT CACAGAGGAA GGTCTAGTTA ACTTCTCAAA GATGAGAATG 
ATTTCTCACA TCATACGAGA GATCCGACAG TTCCAGCAAA CTCCCTACAG AATAGAGCAC 
CAACCAAAGG TCACGCAGTA TCTTCTGGAT AAGACCTTGA TTATGGATGA GGACACACTT 
TATGATTTAT CACTCAAGAT TGAACCCAGG CTTCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF2B ( XM_009301774.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009300049.1 Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa41973
Clone ID Related Accession (Same CDS sequence)	XM_009301774.3 , XM_009301774.2
Accession Version	XM_009301774.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-specific guanine nucleotide-releasing factor 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGTCCAGA CGGCTGAGAA CTCACGCTGC TCTGTTTCTC CAGCCTCTGC GTTTGCCATC 
GCCACTGCAG CAGCTGGACA CAGCAGCCCT CCAGTTTTTA ACAACTCTGA GAGGACTTGT 
GACAAGGAGT TCATTATACG TCGAGCGGCT ACTAACAGAG TCCTGAATGT CTTGCGACAC 
TGGGTCTCCA AACACTCACA GGACTTTGAA ATGAATGGAG AGTTGAAGAT GGGTGTGATC 
TGCCTGCTGG AAGAGGTGTT GAGAGACCCA GACCTTCTCC CTCAGGAGAG AAAAGCCACA 
GCAAACATAC TAAGTGCCCT TTCTCAAGAT GACCAGGATG ATGCACAGCT GAAGATTGAA 
GACATTCTTC AGATGGCTGA GTGCCCAAAG GCCGAGTGTT TTGAGTCTCT GTCTGCAATG 
GAGATAGCAG AACAAATCAC TCTCCTCGAT CACATCGTGT TCAGGAGTAT TCCCTACGAG 
GAGTTCCTGG GCCAAGGATG GATGAAGACA GATAAGACAG AGAGAACGCC ATACATCATG 
AAGACAAGTC AACACTTCAA TGACATGAGT AATCTGGTGG CCTCTCAGAT CATGAGCCAT 
ACAGATGTGG GCTCCAGAGC GGGTTCTATT GAAAAATGGG TCGCAGTGGC TGACATTTGC 
CGTTGTCTCA ACAATTATAA CGGTGTCTTG GAGATCACAT CTGCCCTGAA CCGCAGCGCC 
ATCTACAGGC TGAAGAAGAC ATGGGCTAAA GTCTGCAAAC AGACTAAAGC ACTAATGGAC 
AAACTTCAGA AGACCGTGTC ATCTGAGGGA AGATTTAAAA ACTTGAGGGA AACTCTCAAA 
AACTGTAATC CACCCTGTGT TCCATATCTG GGCATGTACT TGACAGACCT TGCGTTTATC 
GAAGAGGGAA CTCCAAATTT CACAGAGGAA GGTCTAGTTA ACTTCTCAAA GATGAGAATG 
ATTTCTCACA TCATACGAGA GATCCGACAG TTCCAGCAAA CTCCCTACAG AATAGAGCAC 
CAACCAAAGG TCACGCAGTA TCTTCTGGAT AAGACCTTGA TTATGGATGA GGACACACTT 
TATGATTTAT CACTCAAGAT TGAACCCAGG CTTCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300049.1
CDS	377..4078
Translation	MQKSVRYNEGHALFLSVIARKEGTKRGYLSKKTTENSKWHEKFFALYQNVLFYFDTDQSARPSGIYLLEGCTCERVPALKVSTVGKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPYHVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDANDEDAKNSGQVFGHLDFKIVVEPSDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKPFTSIPVRVQYHSFDRLSITSICPDYSLKIRKIALDQSKSLELFFATNQNNRSGDHVNDKSPRLCRKFSSPPPLSISSRTSSPVRTRKLSLNSPIGSKVGILDLSTTSSSAASSPTSANPTISPPPSNNNNNSKPPLDLSRGQSPSSPEQSPGALDDNAEVPRIDALCGKLRRSIRRVQTAENSRCSVSPASAFAIATAAAGHSSPPVFNNSERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFEMNGELKMGVICLLEEVLRDPDLLPQERKATANILSALSQDDQDDAQLKIEDILQMAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPRLPA

Target ORF information:

RefSeq Version	XM_009301774.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ras protein-specific guanine nucleotide-releasing factor 2b (rasgrf2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301774.2

ORF Insert Sequence:

ATGCAGAAGA GTGTTCGGTA CAATGAGGGA CACGCTTTGT TTCTCTCCGT TATCGCGCGT 
AAAGAGGGCA CCAAGCGAGG CTATCTCAGC AAGAAGACCA CGGAAAACAG CAAATGGCAC 
GAGAAGTTTT TTGCACTTTA TCAGAATGTT TTGTTTTACT TCGACACCGA CCAAAGTGCT 
CGACCATCAG GGATTTATCT TCTGGAGGGA TGCACATGTG AACGGGTCCC TGCGCTCAAG 
GTGTCCACTG TTGGCAAAGA CGCACTTGAC AAGCTGCAAT ATTTCCTTGT GGTCTTCGGC 
CATGATGGAC AGAAGCCTCT GGAGCTACGG ACGGAAGAAG AGTCTGACTG TGATGAATGG 
GTTGAAGCCA TCCAGCAGGC CAGCTACTCT GATATCATCA TCGAGCGTGA GGTTCTCATG 
CAGAAATACA TCCACCTGGT GCAAATCGTG GAGACGGAGA AAGTGGCAGC AAACCAGCTC 
CGCACACAAC TGGAGGACCA AGACACTGAG ATTGAGAGGC TTAAAGCTGA GATTATAGCC 
TTGAACAAAA CCAAGGAAAG AATGCGCCCC TACCATGTAT TTCAAGAAGA GGAAGATCCT 
GATATAAAAA AAATTAAAAA GGTCCAGAGC TTCATGCGAG GGTGGCTGTG TAGAAGGAAG 
TGGAAGATTA TTGTGCAGGA CTACATCTGT TCTCCTCATG CTGAGAGCAT GAGGAAGAGG 
AATCAGATCG TCTTCAACAT GGTGGAGGCT GAGACTGAGT ACGTCCATCA GCTTTCCATT 
TTAGTCAACT GCTTCCTGAG GCCACTCAGA ATGGCCGCCA GCTCCAAGAA ACCACCTATA 
AGCCATGATG ATGTCAGTAG CATATTCCTT AACAGCGAGA CAATCATGTT TCTACATGAA 
ATATTCCATC AGGGATTGAA GGCGAGGATA GCAAACTGGC CCACGCTGGT TCTAGCGGAC 
CTCTTTGATA TCTTGCTGCC AATGCTGAAC ATCTATCAGG AGTTTGTGAG GAACCACCAG 
TACAGTCTTC AGGTTCTTGC AAACTGCAAA CAGAACCGGG ACTTTGATAA ACTCCTGAAG 
CAGTATGAAT CCAATGCTGC GTGTGAGGGA CGGATGCTGG AAACATTCCT CACATACCCC 
ATGTTTCAGA TCCCTCGTTA CATCATTACT CTCCATGAGC TATTGGCTCA TACTCCTCAT 
GAGCATGTGG AAAGGAAAAG TCTAGAATTT GCCAAGTCCA AACTGGAGGA GCTTTCCAGG 
GTGATGCACG ATGAAGTAAG TGATACCGAA AACATCCGTA AGAACCTGGC TATTGAGCGC 
ATGATCGTGG AGGGCTGCGA CATCCTCCTT GACACCAGCC AGACGTTTGT GAGACAAGGC 
TCTTTGATCC AATTGCCCTC AGTGGAGCGA GGGAAGCTCA GTAAGGTGCG ACTCGGCTCT 
CTCTCGCTCA AAAAGGAAGG AGAAAGACAG TGCTTCCTTT TTACCAAGCA CTTCCTTATC 
TGCACACGCA GCTCAGGAGG CAAACTTCAC CTGCTCAAAC AAGGTGGTGT GCTGTCCCTG 
ATTGAGTGCA CTCTCATCGA AGAGCCTGAT GCCAATGACG AGGATGCTAA GAATTCAGGG 
CAGGTGTTCG GTCACCTTGA CTTTAAGATT GTGGTGGAGC CTTCAGATGC TCCGGCCTTC 
ACTGTGGTGC TACTGGCACC ATCCCGTCAG GAGAAAGCAG CTTGGACCAG CGACATCAGC 
CAGTGCATCG ATAACATCCG CTGCAATGGC CTGATGACCA GTGTGTTTGA GGAGAACTCA 
AAAGTCACAG TACCTCATAT GATCAAATCT GATGCGCGTC TCCATAAAGA TGATGTTGAC 
ATTTGCTTCA GTAAGACCCT CAACTCCTGC AAGGTTCCTC AGATCCGCTA CGCCAGCGTG 
GAGAGACTGC TGGAGAGGTT GACCGACCTG CGCTTCCTCT CCATAGACTT CCTCAACACA 
TTCCTGCACA CCTACCGCAT CTTCACTACT GCCACCGTGG TCATGGAGAA ACTGGCTGAC 
ATCTACAAGA AGCCTTTTAC TTCCATACCA GTCAGGGTCC AGTATCACTC ATTTGACCGC 
TTGTCCATTA CATCCATCTG CCCAGACTAC AGCTTGAAGA TCAGGAAGAT AGCGCTGGAT 
CAGTCAAAAT CCTTGGAGCT TTTCTTTGCC ACCAATCAGA ACAACCGCAG CGGGGACCAC 
GTCAATGACA AATCTCCAAG ACTGTGCCGC AAGTTCTCAT CCCCTCCACC TCTCTCCATT 
TCCTCTCGTA CTTCCTCTCC AGTTCGCACT AGAAAACTCT CTCTTAATTC ACCTATTGGC 
TCAAAAGTAG GCATCCTTGA TCTGTCCACC ACTTCATCAT CTGCAGCAAG CAGTCCAACC 
TCCGCCAACC CCACCATCTC ACCTCCACCA TCCAACAACA ATAACAACAG CAAGCCACCT 
TTGGATCTTA GCCGTGGACA GAGTCCTTCC TCCCCCGAGC AGAGTCCTGG AGCTCTGGAC 
GACAATGCAG AGGTGCCAAG AATCGATGCC CTTTGTGGTA AACTACGCCG CAGCATAAGG 
AGAGTCCAGA CGGCTGAGAA CTCACGCTGC TCTGTTTCTC CAGCCTCTGC GTTTGCCATC 
GCCACTGCAG CAGCTGGACA CAGCAGCCCT CCAGTTTTTA ACAACTCTGA GAGGACTTGT 
GACAAGGAGT TCATTATACG TCGAGCGGCT ACTAACAGAG TCCTGAATGT CTTGCGACAC 
TGGGTCTCCA AACACTCACA GGACTTTGAA ATGAATGGAG AGTTGAAGAT GGGTGTGATC 
TGCCTGCTGG AAGAGGTGTT GAGAGACCCA GACCTTCTCC CTCAGGAGAG AAAAGCCACA 
GCAAACATAC TAAGTGCCCT TTCTCAAGAT GACCAGGATG ATGCACAGCT GAAGATTGAA 
GACATTCTTC AGATGGCTGA GTGCCCAAAG GCCGAGTGTT TTGAGTCTCT GTCTGCAATG 
GAGATAGCAG AACAAATCAC TCTCCTCGAT CACATCGTGT TCAGGAGTAT TCCCTACGAG 
GAGTTCCTGG GCCAAGGATG GATGAAGACA GATAAGACAG AGAGAACGCC ATACATCATG 
AAGACAAGTC AACACTTCAA TGACATGAGT AATCTGGTGG CCTCTCAGAT CATGAGCCAT 
ACAGATGTGG GCTCCAGAGC GGGTTCTATT GAAAAATGGG TCGCAGTGGC TGACATTTGC 
CGTTGTCTCA ACAATTATAA CGGTGTCTTG GAGATCACAT CTGCCCTGAA CCGCAGCGCC 
ATCTACAGGC TGAAGAAGAC ATGGGCTAAA GTCTGCAAAC AGACTAAAGC ACTAATGGAC 
AAACTTCAGA AGACCGTGTC ATCTGAGGGA AGATTTAAAA ACTTGAGGGA AACTCTCAAA 
AACTGTAATC CACCCTGTGT TCCATATCTG GGCATGTACT TGACAGACCT TGCGTTTATC 
GAAGAGGGAA CTCCAAATTT CACAGAGGAA GGTCTAGTTA ACTTCTCAAA GATGAGAATG 
ATTTCTCACA TCATACGAGA GATCCGACAG TTCCAGCAAA CTCCCTACAG AATAGAGCAC 
CAACCAAAGG TCACGCAGTA TCTTCTGGAT AAGACCTTGA TTATGGATGA GGACACACTT 
TATGATTTAT CACTCAAGAT TGAACCCAGG CTTCCTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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RASGRF2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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