AUS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	AUS1
Entrez Gene ID	854175
Full Name	ATP-binding cassette sterol transporter AUS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183430.1	NP_014654.1	ATP-binding cassette sterol transporter AUS1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR326W	NP_985873.2
Saccharomyces cerevisiae (baker's yeast)	PDR11	NP_012252.1
Saccharomyces cerevisiae (baker's yeast)	AUS1	NP_014654.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Are2p, Aus1p, and Pdr11p, unlike the minor acyltransferase, Are1p, colocalize to sterol and sphingolipid-enriched, detergent-resistant microdomains (DRMs).
Title: ATP-binding cassette transporters and sterol O-acyltransferases interact at membrane microdomains to modulate sterol uptake and esterification.

there is a direct interaction between Aus1 and phosphatidylserien that is critical for the activity of the transporter
Title: The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.

the entry of sterol molecules into plasma membrane is not spontaneous but requires assistance of two ABC (ATP-binding cassette) pumps--Aus1p or Pdr11p.
Title: The role of ABC proteins Aus1p and Pdr11p in the uptake of external sterols in yeast: dehydroergosterol fluorescence study.

Aus1 and Pdr11 are required for sterol uptake.
Title: Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae.

The following AUS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AUS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03327	NM_001183430.1 Latest version!	Saccharomyces cerevisiae S288C ATP-binding cassette sterol transporter AUS1 (AUS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AUS1 ( NM_001183430.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014654.1 Saccharomyces cerevisiae S288C ATP-binding cassette sterol transporter AUS1 (AUS1), partial mRNA.

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CloneID	OSi03327
Clone ID Related Accession (Same CDS sequence)	NM_001183430.1
Accession Version	NM_001183430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-binding cassette sterol transporter AUS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAATTT CAAAGTACTT CACTCCCGTT GCTGACGGGT CACTCACTTT CAATGGCGCG 
AACATTCAAT TTGGCGCCGA TGCTCAAGGC GAGTCAAAAA AGAGTTATGA CGCTGAGGAC 
AGCATGCCGA ATCCGGCAAA TCAACTAAAT GACATAACCT TCCAAGCAGA GGCTGGTGAA 
ATGGTTTTGG TTTTGGGTTA TCCCACATCC ACTCTATTTA AGACTTTGTT TCATGGTAAA 
ACTAGTTTGT CATACTCTCC TCCAGGCTCG ATTAAATTTA AAAATAATGA GTTTAAGAGC 
TTTTCCGAAA AATGTCCCCA CCAAATCATT TACAATAATG AACAAGATGT GCATTTCCCA 
TTTCTAACGG TAGAACAAAC AATTGATTTT GCCTTGAGTT GTAAGTTCGA TATTCCAAAA 
GGTGAGCGCG ATCAAATAAG AAATGAACTT CTAAGAGAAT TCGGCCTGTC TCATGTATTG 
AAAACTATTG TAGGAAATGA TTTTTTCCGT GGTGTTTCTG GTGGTGAGCG TAAACGTATT 
TCTATTATTG AAACGTTTAT TGCTAATGGT TCCGTTTATC TATGGGATAA TTCTACTAAA 
GGTTTAGATT CCGCCACAGC TCTCGATTTT TTGGAAATTC TTAGAAAAAT GGCAAAAGCT 
ACTCGTTCTG TAAACTTAGT CAGAATTTCC CAGGCAAGTG ATAAAATTGT TGATAAGTTT 
GACAAGATTC TTATGCTATC CGATTCCTAC CAGCTTTTCT ATGGTACGGT CGATGAGTGT 
TTGACTTATT TTCGTGACAC TTTAGGTATT GAGAAAGATC CTAACGATTG TATTATTGAA 
TATCTGACCT CTATCTTAAA TTTTCAGTTC AAAAATAAAA ATTTGGGGAA TTTATCAAAT 
TCATCATCTG CTAGCGTTCT CAAAACCGCA ACAGGGGAAG TCACTAAGTA TACTTATAAT 
TCTGATTTCG ATTTATATGA TCAATGGAAA CATTCTTCGT ACTATAGAAA TATAAAGCAG 
CAAATCCAGG GGTCTTCAAT TGATGACTCC ATTAAGGAAG TGGATCCCTC TGATGTCTCA 
CCTATTTTTA ATATTCCGTT GAAGAAACAA TTATTATTTT GCACAAAAAG AGCTTTTCAA 
CGAAGTTTGG GTGATAAAGC TTATATGACG GCACAATTTA TTTCTGTCGT TATTCAATCT 
TTGGTCATTG GTTCACTGTT TTACGAAATT CCGTTGACTA CCATTGGTTC ATACTCAAGA 
GGTTCTTTAA CCTTTTTCTC CATTCTTTTC TTCACTTTTC TTTCTCTTGC AGATATGCCT 
ATTGCCTTCC AAAGACAACC CGTCGTTAAA AAGCAATCCC AACTTCACTT CTATACTAAC 
TGGGTTGAAA CCCTTTCAAC AACAGTGTTC GACTACTGCT TTAAACTTTG TTTGGTAATT 
GTATTCAGTA TCATCCTATA CTTCCTTGCT CACCTGCAAT ACAAGGCTGC AAGATTTTTC 
ATTTTCCTCC TGTTCCTTTC CTTTTACAAT TTCTGTATGG TGTCCTTATT CGCTTTGACG 
ACACTAGTTG CTCCAACCAT TTCAGTTGCA AATTTATTTG CAGGTATTCT GCTACTAGCT 
ATAGCAATGT ATGCTTCTTA CGTCATCTAC CTGAAAAATA TGCATCCTTG GTTTGTATGG 
ATTGCTTATC TAAATCCTGC AATGTACGCT ATGGAGGCAA TACTGTCTAA TGAGCTTTAC 
AATTTGAAGC TGGATTGTAG TGAGACAATT GTTCCAAGAG GTCCTACTTA CAACGATGTT 
CCATTTTCCC ATAAGGCCTG TGCTTGGCAA GGTGCTACTC TGGGTAATGA TTACGTTAGA 
GGCCGTGATT ACTTGAAGCA AGGTTTATCC TACACGTATC ATCATGTGTG GAGAAACTTT 
GGTATTATCA TTGGTTTCCT AGTATTCTTT ATTGCCTGTA CTCTGTTTGC ATCTCAATAT 
ATTAAGCCTT ATTTCAATAA GGATGAAATA GAGCGCAACA ATAGTCGTTT GACAAGATGG 
CTACCATTTT TGAATAAAAA GAGAGGTACC AGGTCCTCTG CAAGAAATGA CAGTAAGTAT 
GTTGGTATCC CAAAGTCGCA CTCCGTTTCG TCTTCCTCGT CTAGCCTGTC GGCTGTTCCA 
TATCAGATAT CACCCTCAAA TAAGGAAATG GCTCTAAACG ATTATAACGA GCAACCTATC 
ACGGAAACAG TAGAGACTCA AAAGCATATC ATCTCTTGGA AAAATATCAA CTACACAGTT 
GGTACAAAGA AACTGATTAA TAATGCATCC GGTTTCATCA GTTCTGGTTT GACCGCCTTA 
ATGGGTGAGT CCGGTGCAGG TAAAACTACT TTGCTGAATG TCTTATCACA AAGAGTTGAG 
ACAGGTGTTG TTAGCGGCGA AATTTTGATC GATGGCCACC CATTGACAGA TGAGGACGCG 
TTTAAGAGAA GTATTGGTTT TGTTCAACAA CAGGATTTGC ATCTAGATTT GCTGTCTGTG 
AAAGAATCTC TGGAAATATC ATGTCTTTTG AGAGGTGATG GTGACAGGGC ATACTTAGAC 
ACTGTTTCAA ATTTACTGAA ATTGCCATCT GACATTTTAG TCGCTGATTT AAACCCGACT 
CAAAGGAAGC TTTTATCTAT CGGTGTTGAA CTTGTTACTA AGCCTTCACT TTTATTATTC 
CTAGACGAAC CAACCTCTGG GCTGGACGCT GAGGCTGCAC TAACAATCGT CAAATTTTTA 
AAACAACTTT CTTTACAGGG TCAGGCTATT TTTTGTACCA TTCATCAGCC TAGTAAAAGT 
GTCATCAGCC ATTTTGACAA TATTTTCTTA TTGAAAAGGG GTGGTGAATG TGTCTTCTTT 
GGACCAATGG ACGATGCCTG CGGCTATTTC ATGTCTCATG ACAACACGCT CGTTTACGAT 
AAAGAACATG ATAACCCTGC AGATTTCGTC ATTGATGCAG TAGGTAACAG TAACTCCTCG 
GCAGGGAAAG ATACAGCGGA GGAAGCTCTT ACTTTGAACA AAGAAGCCAT AGATTGGAGT 
GCATTATGGG AATCTTCTGT AGAAAAGAAA CTAGTTAAAA AAGAAACAGC TAGGTTAGAA 
GATGATGCTC GTGCGTCTGG TGTTGATTAC ACAACCTCAT TGTGGAAGCA GCCTTCCTAC 
TTACAGCAAT TAGCTCTTAT TACAAGAAGA CAATATATTT GTACGAAAAG AGACATGACT 
TATGTCATGG CCAAGTACTG TTTGAATGGT GGCGCTGGTT TGTTTATCGG TTTCTCATTT 
TGGCACATTA AGCATAATAT TATCGGTTTG CAGGATAGTA TCTTCTTCTG TTTCATGGCT 
CTTTGTGTTT CATCTCCATT GATCAATCAA ATTCAGGACA AAGCCTTGAA AACAAAGGAA 
GTTTATGTTG CTAGGGAGGC GAGATCTAAC ACTTATCACT GGACTGTTCT TCTTTTGTCA 
CAGTCAATTA TTGAACTACC ACTGGCACTT ACAAGTTCCA CACTATTTTT CGTCTGTGCC 
TTTTTCTCTT GTGGATTTAA CAACGCTGGC TGGAGTGCCG GTGTTTTCTT TTTAAACTAT 
ATGCTTTTCG CTGCTTATTA CTCGACTCTT GGTTTATGGC TGATCTATAC TGCTCCTAAT 
CTACAAACTG CTGCTGTTTT TGTTGCGTTT ATTTACAGTT TTACAGCATC ATTCTGTGGT 
GTTATGCAAC CATACTCTTT GTTTCCAACT TTTTGGAAAT TCATGTACAG GGTTTCGCCA 
TACACATATT TTGTGGAAAC TTTTGTTAGT ATTCTATTGC ATAACTGGGA AATCAAGTGT 
GACATGTCGG AGATGGTTCC TGGTCAACCT CTGACGGGGC AATCTTGTGG CCAATTTATG 
GAAGCTTTCA TAGAAGAATA CGGTGGCTAT CTACATAATA AGAACACTTT CACAGTCTGC 
GCTTATTGTA CGTACACTGT TGGCGATGAC TTCCTGAAAA ATGAGAATAT GAGTTACGAC 
CACGTTTGGA GGAACTTTGG TATTGAATGG GCTTTTGTTG GTTTCAATTT CTTTGCGATG 
TTTGCTGGTT ACTACTTAAC TTACGTGGCA AGAATATGGC CAAAGGTCTT TAAAATTATC 
ACCAAAGTAA TTCCACACAG AGGGAAGAAG CCTGTACAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014654.1
CDS	1..4185
Translation	MSISKYFTPVADGSLTFNGANIQFGADAQGESKKSYDAEDSMPNPANQLNDITFQAEAGEMVLVLGYPTSTLFKTLFHGKTSLSYSPPGSIKFKNNEFKSFSEKCPHQIIYNNEQDVHFPFLTVEQTIDFALSCKFDIPKGERDQIRNELLREFGLSHVLKTIVGNDFFRGVSGGERKRISIIETFIANGSVYLWDNSTKGLDSATALDFLEILRKMAKATRSVNLVRISQASDKIVDKFDKILMLSDSYQLFYGTVDECLTYFRDTLGIEKDPNDCIIEYLTSILNFQFKNKNLGNLSNSSSASVLKTATGEVTKYTYNSDFDLYDQWKHSSYYRNIKQQIQGSSIDDSIKEVDPSDVSPIFNIPLKKQLLFCTKRAFQRSLGDKAYMTAQFISVVIQSLVIGSLFYEIPLTTIGSYSRGSLTFFSILFFTFLSLADMPIAFQRQPVVKKQSQLHFYTNWVETLSTTVFDYCFKLCLVIVFSIILYFLAHLQYKAARFFIFLLFLSFYNFCMVSLFALTTLVAPTISVANLFAGILLLAIAMYASYVIYLKNMHPWFVWIAYLNPAMYAMEAILSNELYNLKLDCSETIVPRGPTYNDVPFSHKACAWQGATLGNDYVRGRDYLKQGLSYTYHHVWRNFGIIIGFLVFFIACTLFASQYIKPYFNKDEIERNNSRLTRWLPFLNKKRGTRSSARNDSKYVGIPKSHSVSSSSSSLSAVPYQISPSNKEMALNDYNEQPITETVETQKHIISWKNINYTVGTKKLINNASGFISSGLTALMGESGAGKTTLLNVLSQRVETGVVSGEILIDGHPLTDEDAFKRSIGFVQQQDLHLDLLSVKESLEISCLLRGDGDRAYLDTVSNLLKLPSDILVADLNPTQRKLLSIGVELVTKPSLLLFLDEPTSGLDAEAALTIVKFLKQLSLQGQAIFCTIHQPSKSVISHFDNIFLLKRGGECVFFGPMDDACGYFMSHDNTLVYDKEHDNPADFVIDAVGNSNSSAGKDTAEEALTLNKEAIDWSALWESSVEKKLVKKETARLEDDARASGVDYTTSLWKQPSYLQQLALITRRQYICTKRDMTYVMAKYCLNGGAGLFIGFSFWHIKHNIIGLQDSIFFCFMALCVSSPLINQIQDKALKTKEVYVAREARSNTYHWTVLLLSQSIIELPLALTSSTLFFVCAFFSCGFNNAGWSAGVFFLNYMLFAAYYSTLGLWLIYTAPNLQTAAVFVAFIYSFTASFCGVMQPYSLFPTFWKFMYRVSPYTYFVETFVSILLHNWEIKCDMSEMVPGQPLTGQSCGQFMEAFIEEYGGYLHNKNTFTVCAYCTYTVGDDFLKNENMSYDHVWRNFGIEWAFVGFNFFAMFAGYYLTYVARIWPKVFKIITKVIPHRGKKPVQN

Target ORF information:

RefSeq Version	NM_001183430.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-binding cassette sterol transporter AUS1 (AUS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183430.1

ORF Insert Sequence:

ATGTCAATTT CAAAGTACTT CACTCCCGTT GCTGACGGGT CACTCACTTT CAATGGCGCG 
AACATTCAAT TTGGCGCCGA TGCTCAAGGC GAGTCAAAAA AGAGTTATGA CGCTGAGGAC 
AGCATGCCGA ATCCGGCAAA TCAACTAAAT GACATAACCT TCCAAGCAGA GGCTGGTGAA 
ATGGTTTTGG TTTTGGGTTA TCCCACATCC ACTCTATTTA AGACTTTGTT TCATGGTAAA 
ACTAGTTTGT CATACTCTCC TCCAGGCTCG ATTAAATTTA AAAATAATGA GTTTAAGAGC 
TTTTCCGAAA AATGTCCCCA CCAAATCATT TACAATAATG AACAAGATGT GCATTTCCCA 
TTTCTAACGG TAGAACAAAC AATTGATTTT GCCTTGAGTT GTAAGTTCGA TATTCCAAAA 
GGTGAGCGCG ATCAAATAAG AAATGAACTT CTAAGAGAAT TCGGCCTGTC TCATGTATTG 
AAAACTATTG TAGGAAATGA TTTTTTCCGT GGTGTTTCTG GTGGTGAGCG TAAACGTATT 
TCTATTATTG AAACGTTTAT TGCTAATGGT TCCGTTTATC TATGGGATAA TTCTACTAAA 
GGTTTAGATT CCGCCACAGC TCTCGATTTT TTGGAAATTC TTAGAAAAAT GGCAAAAGCT 
ACTCGTTCTG TAAACTTAGT CAGAATTTCC CAGGCAAGTG ATAAAATTGT TGATAAGTTT 
GACAAGATTC TTATGCTATC CGATTCCTAC CAGCTTTTCT ATGGTACGGT CGATGAGTGT 
TTGACTTATT TTCGTGACAC TTTAGGTATT GAGAAAGATC CTAACGATTG TATTATTGAA 
TATCTGACCT CTATCTTAAA TTTTCAGTTC AAAAATAAAA ATTTGGGGAA TTTATCAAAT 
TCATCATCTG CTAGCGTTCT CAAAACCGCA ACAGGGGAAG TCACTAAGTA TACTTATAAT 
TCTGATTTCG ATTTATATGA TCAATGGAAA CATTCTTCGT ACTATAGAAA TATAAAGCAG 
CAAATCCAGG GGTCTTCAAT TGATGACTCC ATTAAGGAAG TGGATCCCTC TGATGTCTCA 
CCTATTTTTA ATATTCCGTT GAAGAAACAA TTATTATTTT GCACAAAAAG AGCTTTTCAA 
CGAAGTTTGG GTGATAAAGC TTATATGACG GCACAATTTA TTTCTGTCGT TATTCAATCT 
TTGGTCATTG GTTCACTGTT TTACGAAATT CCGTTGACTA CCATTGGTTC ATACTCAAGA 
GGTTCTTTAA CCTTTTTCTC CATTCTTTTC TTCACTTTTC TTTCTCTTGC AGATATGCCT 
ATTGCCTTCC AAAGACAACC CGTCGTTAAA AAGCAATCCC AACTTCACTT CTATACTAAC 
TGGGTTGAAA CCCTTTCAAC AACAGTGTTC GACTACTGCT TTAAACTTTG TTTGGTAATT 
GTATTCAGTA TCATCCTATA CTTCCTTGCT CACCTGCAAT ACAAGGCTGC AAGATTTTTC 
ATTTTCCTCC TGTTCCTTTC CTTTTACAAT TTCTGTATGG TGTCCTTATT CGCTTTGACG 
ACACTAGTTG CTCCAACCAT TTCAGTTGCA AATTTATTTG CAGGTATTCT GCTACTAGCT 
ATAGCAATGT ATGCTTCTTA CGTCATCTAC CTGAAAAATA TGCATCCTTG GTTTGTATGG 
ATTGCTTATC TAAATCCTGC AATGTACGCT ATGGAGGCAA TACTGTCTAA TGAGCTTTAC 
AATTTGAAGC TGGATTGTAG TGAGACAATT GTTCCAAGAG GTCCTACTTA CAACGATGTT 
CCATTTTCCC ATAAGGCCTG TGCTTGGCAA GGTGCTACTC TGGGTAATGA TTACGTTAGA 
GGCCGTGATT ACTTGAAGCA AGGTTTATCC TACACGTATC ATCATGTGTG GAGAAACTTT 
GGTATTATCA TTGGTTTCCT AGTATTCTTT ATTGCCTGTA CTCTGTTTGC ATCTCAATAT 
ATTAAGCCTT ATTTCAATAA GGATGAAATA GAGCGCAACA ATAGTCGTTT GACAAGATGG 
CTACCATTTT TGAATAAAAA GAGAGGTACC AGGTCCTCTG CAAGAAATGA CAGTAAGTAT 
GTTGGTATCC CAAAGTCGCA CTCCGTTTCG TCTTCCTCGT CTAGCCTGTC GGCTGTTCCA 
TATCAGATAT CACCCTCAAA TAAGGAAATG GCTCTAAACG ATTATAACGA GCAACCTATC 
ACGGAAACAG TAGAGACTCA AAAGCATATC ATCTCTTGGA AAAATATCAA CTACACAGTT 
GGTACAAAGA AACTGATTAA TAATGCATCC GGTTTCATCA GTTCTGGTTT GACCGCCTTA 
ATGGGTGAGT CCGGTGCAGG TAAAACTACT TTGCTGAATG TCTTATCACA AAGAGTTGAG 
ACAGGTGTTG TTAGCGGCGA AATTTTGATC GATGGCCACC CATTGACAGA TGAGGACGCG 
TTTAAGAGAA GTATTGGTTT TGTTCAACAA CAGGATTTGC ATCTAGATTT GCTGTCTGTG 
AAAGAATCTC TGGAAATATC ATGTCTTTTG AGAGGTGATG GTGACAGGGC ATACTTAGAC 
ACTGTTTCAA ATTTACTGAA ATTGCCATCT GACATTTTAG TCGCTGATTT AAACCCGACT 
CAAAGGAAGC TTTTATCTAT CGGTGTTGAA CTTGTTACTA AGCCTTCACT TTTATTATTC 
CTAGACGAAC CAACCTCTGG GCTGGACGCT GAGGCTGCAC TAACAATCGT CAAATTTTTA 
AAACAACTTT CTTTACAGGG TCAGGCTATT TTTTGTACCA TTCATCAGCC TAGTAAAAGT 
GTCATCAGCC ATTTTGACAA TATTTTCTTA TTGAAAAGGG GTGGTGAATG TGTCTTCTTT 
GGACCAATGG ACGATGCCTG CGGCTATTTC ATGTCTCATG ACAACACGCT CGTTTACGAT 
AAAGAACATG ATAACCCTGC AGATTTCGTC ATTGATGCAG TAGGTAACAG TAACTCCTCG 
GCAGGGAAAG ATACAGCGGA GGAAGCTCTT ACTTTGAACA AAGAAGCCAT AGATTGGAGT 
GCATTATGGG AATCTTCTGT AGAAAAGAAA CTAGTTAAAA AAGAAACAGC TAGGTTAGAA 
GATGATGCTC GTGCGTCTGG TGTTGATTAC ACAACCTCAT TGTGGAAGCA GCCTTCCTAC 
TTACAGCAAT TAGCTCTTAT TACAAGAAGA CAATATATTT GTACGAAAAG AGACATGACT 
TATGTCATGG CCAAGTACTG TTTGAATGGT GGCGCTGGTT TGTTTATCGG TTTCTCATTT 
TGGCACATTA AGCATAATAT TATCGGTTTG CAGGATAGTA TCTTCTTCTG TTTCATGGCT 
CTTTGTGTTT CATCTCCATT GATCAATCAA ATTCAGGACA AAGCCTTGAA AACAAAGGAA 
GTTTATGTTG CTAGGGAGGC GAGATCTAAC ACTTATCACT GGACTGTTCT TCTTTTGTCA 
CAGTCAATTA TTGAACTACC ACTGGCACTT ACAAGTTCCA CACTATTTTT CGTCTGTGCC 
TTTTTCTCTT GTGGATTTAA CAACGCTGGC TGGAGTGCCG GTGTTTTCTT TTTAAACTAT 
ATGCTTTTCG CTGCTTATTA CTCGACTCTT GGTTTATGGC TGATCTATAC TGCTCCTAAT 
CTACAAACTG CTGCTGTTTT TGTTGCGTTT ATTTACAGTT TTACAGCATC ATTCTGTGGT 
GTTATGCAAC CATACTCTTT GTTTCCAACT TTTTGGAAAT TCATGTACAG GGTTTCGCCA 
TACACATATT TTGTGGAAAC TTTTGTTAGT ATTCTATTGC ATAACTGGGA AATCAAGTGT 
GACATGTCGG AGATGGTTCC TGGTCAACCT CTGACGGGGC AATCTTGTGG CCAATTTATG 
GAAGCTTTCA TAGAAGAATA CGGTGGCTAT CTACATAATA AGAACACTTT CACAGTCTGC 
GCTTATTGTA CGTACACTGT TGGCGATGAC TTCCTGAAAA ATGAGAATAT GAGTTACGAC 
CACGTTTGGA GGAACTTTGG TATTGAATGG GCTTTTGTTG GTTTCAATTT CTTTGCGATG 
TTTGCTGGTT ACTACTTAAC TTACGTGGCA AGAATATGGC CAAAGGTCTT TAAAATTATC 
ACCAAAGTAA TTCCACACAG AGGGAAGAAG CCTGTACAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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AUS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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