DIS3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DIS3
Entrez Gene ID	854138
Full Name	exosome catalytic subunit DIS3
Synonyms	MTR17,RRP44
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183275.1	NP_014621.1	exosome catalytic subunit DIS3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DIS3	XP_542610.3
Magnaporthe oryzae (rice blast fungus)	MGG_00193	XP_003718924.1
Xenopus tropicalis (tropical clawed frog)	dis3	NP_001120564.1
Eremothecium gossypii	AGOS_AEL031C	NP_984830.1
Arabidopsis thaliana (thale cress)	EMB2763	NP_001189541.1
Pan troglodytes (chimpanzee)	DIS3	XP_509678.2
Bos taurus (cattle)	DIS3	XP_002691932.2
Mus musculus (house mouse)	Dis3	NP_082591.2
Gallus gallus (chicken)	DIS3	XP_417016.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005191	XP_314089.3
Caenorhabditis elegans (roundworm)	dis-3	NP_501835.2
Neurospora crassa	NCU03065	XP_963787.1
Saccharomyces cerevisiae (baker's yeast)	DIS3	NP_014621.1
Oryza sativa (rice)	Os03g0129200	NP_001048846.1
Homo sapiens (human)	DIS3	NP_055768.3
	DIS3	XP_001083933.1
Rattus norvegicus (Norway rat)	Dis3	NP_001120955.1
Danio rerio (zebrafish)	dis3	XP_001336850.2
Drosophila melanogaster (fruit fly)	Dis3	NP_001262910.1
Kluyveromyces lactis	KLLA0A10835g	XP_451472.1
Schizosaccharomyces pombe (fission yeast)	dis3	NP_596653.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, supporting the predicted widening of the lumen in the Rrp6-associated.Many exosome substrates exhibited clear preferences for a specific path to Rrp44. Both threading and direct access routes involve the RNA helicase Mtr4
Title: Transcriptome-wide analysis of alternative routes for RNA substrates into the exosome complex.

3' phosphate RNA is not a substrate for Rrp6 but is readily degraded by Rrp44 in the nuclear exosome.
Title: Nuclear RNA Exosome at 3.1?? Reveals Substrate Specificities, RNA Paths, and Allosteric Inhibition of Rrp44/Dis3.

While the molecular functions of Rrp44 and the RNA exosome have been extensively characterized, our studies characterize how this molecular function affects the physiology of the organism.
Title: The RNA exosome affects iron response and sensitivity to oxidative stress.

Rrp41 mutation caused accumulation of nuclear and cytoplasmic exosome substrates including the non-stop decay reporter, for which degradation is dependent on either endonucleolytic or exonucleolytic Dis3 activities.
Title: The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro.

Data shows that Dis3p and Rrp6p from S. cerevisiae strain W303 have both overlapping and specific roles in degrading distinct classes of RNA substrates.
Title: Extensive degradation of RNA precursors by the exosome in wild-type cells.

The following DIS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03295	NM_001183275.1 Latest version!	Saccharomyces cerevisiae S288C exosome catalytic subunit DIS3 (DIS3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIS3 ( NM_001183275.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014621.1 Saccharomyces cerevisiae S288C exosome catalytic subunit DIS3 (DIS3), partial mRNA.

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CloneID	OSi03295
Clone ID Related Accession (Same CDS sequence)	NM_001183275.1
Accession Version	NM_001183275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	exosome catalytic subunit DIS3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGTTC CCGCTATCGC CCCCAGACGG AAGAGACTTG CAGATGGTTT AAGCGTCACT 
CAGAAGGTGT TTGTAAGGTC TCGTAATGGA GGCGCTACCA AGATCGTAAG AGAACACTAT 
TTAAGATCGG ACATCCCATG TCTTTCGAGA AGTTGTACCA AGTGTCCGCA AATTGTCGTC 
CCCGATGCTC AAAATGAGTT GCCGAAGTTC ATCTTGTCAG ATTCTCCCTT AGAATTAAGC 
GCACCCATTG GTAAGCATTA CGTCGTCTTG GATACCAACG TGGTGTTACA GGCAATTGAC 
CTTTTAGAGA ATCCGAATTG TTTTTTCGAC GTCATTGTCC CCCAGATTGT CCTAGATGAA 
GTGAGAAACA AGTCATATCC GGTGTACACG AGATTAAGAA CTCTATGCAG AGATAGTGAC 
GATCATAAGA GATTCATCGT GTTTCATAAC GAATTTAGTG AACATACTTT TGTGGAAAGG 
CTTCCAAATG AAACGATTAA TGACAGAAAC GACAGGGCTA TAAGGAAAAC CTGTCAATGG 
TATAGTGAGC ACTTGAAACC TTATGACATA AACGTCGTTC TTGTTACCAA CGATCGTTTG 
AATAGAGAAG CTGCCACTAA GGAAGTGGAG TCCAATATAA TAACCAAGAG CTTGGTACAG 
TATATCGAGT TACTACCAAA TGCAGACGAC ATCAGAGACT CTATTCCTCA AATGGATTCT 
TTTGATAAAG ATCTTGAAAG GGACACATTT TCAGATTTCA CGTTCCCGGA GTACTATTCT 
ACAGCAAGAG TAATGGGTGG TTTGAAAAAT GGCGTTTTGT ACCAGGGCAA TATTCAAATA 
TCAGAGTACA ACTTTCTAGA AGGTTCAGTG AGTTTACCAA GATTTTCCAA ACCAGTGTTA 
ATTGTTGGGC AGAAAAACCT AAATAGAGCA TTCAACGGTG ACCAGGTTAT CGTGGAACTG 
CTACCTCAAT CAGAATGGAA GGCACCCTCA TCCATTGTCT TGGATTCTGA ACATTTCGAC 
GTTAACGATA ACCCCGATAT TGAGGCTGGC GATGACGATG ACAATAATGA GTCCTCTTCC 
AATACCACCG TGATTTCCGA CAAGCAGCGC AGGCTGCTTG CTAAAGATGC TATGATAGCT 
CAAAGATCGA AAAAAATTCA GCCCACGGCA AAAGTTGTAT ATATACAAAG AAGATCCTGG 
AGACAGTACG TCGGTCAACT AGCACCAAGT TCTGTTGATC CACAAAGCAG TAGTACACAG 
AATGTCTTCG TTATTTTGAT GGACAAATGT CTTCCAAAAG TCAGAATCAG AACAAGGCGT 
GCTGCTGAAT TATTGGATAA GAGGATTGTC ATTTCCATTG ATTCGTGGCC CACCACTCAT 
AAATACCCAT TAGGTCACTT TGTGAGAGAC CTCGGTACAA TTGAATCTGC TCAAGCTGAA 
ACAGAGGCCT TATTGTTGGA GCATGATGTT GAATATAGGC CTTTTTCCAA GAAAGTTCTA 
GAATGTTTGC CCGCAGAAGG CCACGATTGG AAGGCCCCAA CAAAACTGGA CGATCCTGAG 
GCTGTTTCAA AGGATCCATT ATTGACAAAA AGAAAGGATC TCAGAGATAA ACTTATATGT 
AGTATCGATC CTCCAGGATG TGTTGATATT GACGATGCCC TACATGCGAA AAAGCTTCCA 
AACGGTAATT GGGAAGTTGG TGTTCATATT GCTGATGTTA CTCACTTCGT TAAACCGGGC 
ACTGCCCTGG ATGCGGAAGG TGCTGCAAGA GGTACTTCTG TATATTTGGT AGACAAACGT 
ATTGACATGC TGCCCATGCT TCTAGGTACC GACCTGTGTT CTCTAAAACC ATACGTTGAT 
AGATTCGCAT TCTCTGTCAT TTGGGAATTG GATGATAGTG CTAATATTGT AAATGTTAAT 
TTTATGAAAT CCGTCATCAG ATCTAGAGAA GCTTTCTCAT ACGAACAAGC ACAACTGAGA 
ATTGATGACA AAACCCAAAA TGATGAATTA ACGATGGGTA TGAGGGCTCT CTTGAAATTG 
TCTGTAAAAC TGAAGCAAAA GAGACTAGAG GCAGGTGCCT TGAACTTAGC TTCTCCTGAG 
GTTAAGGTCC ATATGGATAG TGAGACTTCA GATCCAAATG AAGTGGAAAT CAAAAAATTA 
CTGGCGACAA ATTCTTTAGT TGAAGAATTT ATGTTGTTGG CTAATATATC AGTGGCAAGG 
AAGATATACG ATGCCTTCCC TCAAACGGCG ATGCTAAGAA GACACGCAGC TCCGCCATCT 
ACCAATTTTG AAATCTTAAA TGAAATGTTA AACACAAGAA AGAATATGTC AATTTCGTTG 
GAATCGTCCA AGGCCTTGGC CGATTCTTTA GACAGGTGTG TGGATCCCGA AGATCCATAT 
TTTAATACAT TGGTTCGTAT CATGTCGACT CGCTGTATGA TGGCAGCCCA ATACTTTTAT 
TCTGGAGCTT ATTCTTATCC TGACTTTAGA CACTATGGTT TAGCCGTTGA TATCTACACA 
CATTTCACAT CACCTATTAG ACGTTACTGT GATGTTGTGG CCCATAGACA ATTAGCAGGT 
GCCATTGGGT ATGAACCCCT AAGTTTGACT CATCGTGATA AGAATAAAAT GGACATGATT 
TGCAGAAATA TCAACAGAAA GCACAGAAAC GCCCAATTCG CCGGTAGGGC AAGCATAGAA 
TACTATGTCG GGCAAGTAAT GAGGAATAAC GAGTCCACAG AAACTGGATA TGTTATTAAG 
GTATTTAATA ATGGTATAGT CGTACTGGTT CCCAAGTTTG GCGTGGAAGG CTTGATAAGA 
CTGGATAATT TGACTGAGGA TCCTAACTCA GCCGCTTTTG ATGAGGTGGA ATACAAATTA 
ACCTTTGTGC CTACAAATTC AGATAAACCG AGGGATGTTT ACGTTTTCGA TAAGGTCGAA 
GTTCAAGTTA GGTCGGTGAT GGATCCAATT ACTAGCAAGC GTAAGGCAGA ATTATTGTTA 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014621.1
CDS	1..3006
Translation	MSVPAIAPRRKRLADGLSVTQKVFVRSRNGGATKIVREHYLRSDIPCLSRSCTKCPQIVVPDAQNELPKFILSDSPLELSAPIGKHYVVLDTNVVLQAIDLLENPNCFFDVIVPQIVLDEVRNKSYPVYTRLRTLCRDSDDHKRFIVFHNEFSEHTFVERLPNETINDRNDRAIRKTCQWYSEHLKPYDINVVLVTNDRLNREAATKEVESNIITKSLVQYIELLPNADDIRDSIPQMDSFDKDLERDTFSDFTFPEYYSTARVMGGLKNGVLYQGNIQISEYNFLEGSVSLPRFSKPVLIVGQKNLNRAFNGDQVIVELLPQSEWKAPSSIVLDSEHFDVNDNPDIEAGDDDDNNESSSNTTVISDKQRRLLAKDAMIAQRSKKIQPTAKVVYIQRRSWRQYVGQLAPSSVDPQSSSTQNVFVILMDKCLPKVRIRTRRAAELLDKRIVISIDSWPTTHKYPLGHFVRDLGTIESAQAETEALLLEHDVEYRPFSKKVLECLPAEGHDWKAPTKLDDPEAVSKDPLLTKRKDLRDKLICSIDPPGCVDIDDALHAKKLPNGNWEVGVHIADVTHFVKPGTALDAEGAARGTSVYLVDKRIDMLPMLLGTDLCSLKPYVDRFAFSVIWELDDSANIVNVNFMKSVIRSREAFSYEQAQLRIDDKTQNDELTMGMRALLKLSVKLKQKRLEAGALNLASPEVKVHMDSETSDPNEVEIKKLLATNSLVEEFMLLANISVARKIYDAFPQTAMLRRHAAPPSTNFEILNEMLNTRKNMSISLESSKALADSLDRCVDPEDPYFNTLVRIMSTRCMMAAQYFYSGAYSYPDFRHYGLAVDIYTHFTSPIRRYCDVVAHRQLAGAIGYEPLSLTHRDKNKMDMICRNINRKHRNAQFAGRASIEYYVGQVMRNNESTETGYVIKVFNNGIVVLVPKFGVEGLIRLDNLTEDPNSAAFDEVEYKLTFVPTNSDKPRDVYVFDKVEVQVRSVMDPITSKRKAELLLK

Target ORF information:

RefSeq Version	NM_001183275.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C exosome catalytic subunit DIS3 (DIS3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183275.1

ORF Insert Sequence:

ATGTCAGTTC CCGCTATCGC CCCCAGACGG AAGAGACTTG CAGATGGTTT AAGCGTCACT 
CAGAAGGTGT TTGTAAGGTC TCGTAATGGA GGCGCTACCA AGATCGTAAG AGAACACTAT 
TTAAGATCGG ACATCCCATG TCTTTCGAGA AGTTGTACCA AGTGTCCGCA AATTGTCGTC 
CCCGATGCTC AAAATGAGTT GCCGAAGTTC ATCTTGTCAG ATTCTCCCTT AGAATTAAGC 
GCACCCATTG GTAAGCATTA CGTCGTCTTG GATACCAACG TGGTGTTACA GGCAATTGAC 
CTTTTAGAGA ATCCGAATTG TTTTTTCGAC GTCATTGTCC CCCAGATTGT CCTAGATGAA 
GTGAGAAACA AGTCATATCC GGTGTACACG AGATTAAGAA CTCTATGCAG AGATAGTGAC 
GATCATAAGA GATTCATCGT GTTTCATAAC GAATTTAGTG AACATACTTT TGTGGAAAGG 
CTTCCAAATG AAACGATTAA TGACAGAAAC GACAGGGCTA TAAGGAAAAC CTGTCAATGG 
TATAGTGAGC ACTTGAAACC TTATGACATA AACGTCGTTC TTGTTACCAA CGATCGTTTG 
AATAGAGAAG CTGCCACTAA GGAAGTGGAG TCCAATATAA TAACCAAGAG CTTGGTACAG 
TATATCGAGT TACTACCAAA TGCAGACGAC ATCAGAGACT CTATTCCTCA AATGGATTCT 
TTTGATAAAG ATCTTGAAAG GGACACATTT TCAGATTTCA CGTTCCCGGA GTACTATTCT 
ACAGCAAGAG TAATGGGTGG TTTGAAAAAT GGCGTTTTGT ACCAGGGCAA TATTCAAATA 
TCAGAGTACA ACTTTCTAGA AGGTTCAGTG AGTTTACCAA GATTTTCCAA ACCAGTGTTA 
ATTGTTGGGC AGAAAAACCT AAATAGAGCA TTCAACGGTG ACCAGGTTAT CGTGGAACTG 
CTACCTCAAT CAGAATGGAA GGCACCCTCA TCCATTGTCT TGGATTCTGA ACATTTCGAC 
GTTAACGATA ACCCCGATAT TGAGGCTGGC GATGACGATG ACAATAATGA GTCCTCTTCC 
AATACCACCG TGATTTCCGA CAAGCAGCGC AGGCTGCTTG CTAAAGATGC TATGATAGCT 
CAAAGATCGA AAAAAATTCA GCCCACGGCA AAAGTTGTAT ATATACAAAG AAGATCCTGG 
AGACAGTACG TCGGTCAACT AGCACCAAGT TCTGTTGATC CACAAAGCAG TAGTACACAG 
AATGTCTTCG TTATTTTGAT GGACAAATGT CTTCCAAAAG TCAGAATCAG AACAAGGCGT 
GCTGCTGAAT TATTGGATAA GAGGATTGTC ATTTCCATTG ATTCGTGGCC CACCACTCAT 
AAATACCCAT TAGGTCACTT TGTGAGAGAC CTCGGTACAA TTGAATCTGC TCAAGCTGAA 
ACAGAGGCCT TATTGTTGGA GCATGATGTT GAATATAGGC CTTTTTCCAA GAAAGTTCTA 
GAATGTTTGC CCGCAGAAGG CCACGATTGG AAGGCCCCAA CAAAACTGGA CGATCCTGAG 
GCTGTTTCAA AGGATCCATT ATTGACAAAA AGAAAGGATC TCAGAGATAA ACTTATATGT 
AGTATCGATC CTCCAGGATG TGTTGATATT GACGATGCCC TACATGCGAA AAAGCTTCCA 
AACGGTAATT GGGAAGTTGG TGTTCATATT GCTGATGTTA CTCACTTCGT TAAACCGGGC 
ACTGCCCTGG ATGCGGAAGG TGCTGCAAGA GGTACTTCTG TATATTTGGT AGACAAACGT 
ATTGACATGC TGCCCATGCT TCTAGGTACC GACCTGTGTT CTCTAAAACC ATACGTTGAT 
AGATTCGCAT TCTCTGTCAT TTGGGAATTG GATGATAGTG CTAATATTGT AAATGTTAAT 
TTTATGAAAT CCGTCATCAG ATCTAGAGAA GCTTTCTCAT ACGAACAAGC ACAACTGAGA 
ATTGATGACA AAACCCAAAA TGATGAATTA ACGATGGGTA TGAGGGCTCT CTTGAAATTG 
TCTGTAAAAC TGAAGCAAAA GAGACTAGAG GCAGGTGCCT TGAACTTAGC TTCTCCTGAG 
GTTAAGGTCC ATATGGATAG TGAGACTTCA GATCCAAATG AAGTGGAAAT CAAAAAATTA 
CTGGCGACAA ATTCTTTAGT TGAAGAATTT ATGTTGTTGG CTAATATATC AGTGGCAAGG 
AAGATATACG ATGCCTTCCC TCAAACGGCG ATGCTAAGAA GACACGCAGC TCCGCCATCT 
ACCAATTTTG AAATCTTAAA TGAAATGTTA AACACAAGAA AGAATATGTC AATTTCGTTG 
GAATCGTCCA AGGCCTTGGC CGATTCTTTA GACAGGTGTG TGGATCCCGA AGATCCATAT 
TTTAATACAT TGGTTCGTAT CATGTCGACT CGCTGTATGA TGGCAGCCCA ATACTTTTAT 
TCTGGAGCTT ATTCTTATCC TGACTTTAGA CACTATGGTT TAGCCGTTGA TATCTACACA 
CATTTCACAT CACCTATTAG ACGTTACTGT GATGTTGTGG CCCATAGACA ATTAGCAGGT 
GCCATTGGGT ATGAACCCCT AAGTTTGACT CATCGTGATA AGAATAAAAT GGACATGATT 
TGCAGAAATA TCAACAGAAA GCACAGAAAC GCCCAATTCG CCGGTAGGGC AAGCATAGAA 
TACTATGTCG GGCAAGTAAT GAGGAATAAC GAGTCCACAG AAACTGGATA TGTTATTAAG 
GTATTTAATA ATGGTATAGT CGTACTGGTT CCCAAGTTTG GCGTGGAAGG CTTGATAAGA 
CTGGATAATT TGACTGAGGA TCCTAACTCA GCCGCTTTTG ATGAGGTGGA ATACAAATTA 
ACCTTTGTGC CTACAAATTC AGATAAACCG AGGGATGTTT ACGTTTTCGA TAAGGTCGAA 
GTTCAAGTTA GGTCGGTGAT GGATCCAATT ACTAGCAAGC GTAAGGCAGA ATTATTGTTA 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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DIS3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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