Dis3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dis3
Entrez Gene ID	42900
Full Name	CG6413 gene product from transcript CG6413-RA
Synonyms	CG6413,Dmel\CG6413,Rrp44,Taz,dDis3,dRrp44,dis3,taz
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95F13-95F14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275981.1	NP_001262910.1	Dis3, isoform B
NM_142989.4	NP_651246.2	Dis3, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DIS3	XP_542610.3
Magnaporthe oryzae (rice blast fungus)	MGG_00193	XP_003718924.1
Xenopus tropicalis (tropical clawed frog)	dis3	NP_001120564.1
Eremothecium gossypii	AGOS_AEL031C	NP_984830.1
Arabidopsis thaliana (thale cress)	EMB2763	NP_001189541.1
Pan troglodytes (chimpanzee)	DIS3	XP_509678.2
Bos taurus (cattle)	DIS3	XP_002691932.2
Mus musculus (house mouse)	Dis3	NP_082591.2
Gallus gallus (chicken)	DIS3	XP_417016.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005191	XP_314089.3
Caenorhabditis elegans (roundworm)	dis-3	NP_501835.2
Neurospora crassa	NCU03065	XP_963787.1
Saccharomyces cerevisiae (baker's yeast)	DIS3	NP_014621.1
Oryza sativa (rice)	Os03g0129200	NP_001048846.1
Homo sapiens (human)	DIS3	NP_055768.3
	DIS3	XP_001083933.1
Rattus norvegicus (Norway rat)	Dis3	NP_001120955.1
Danio rerio (zebrafish)	dis3	XP_001336850.2
Drosophila melanogaster (fruit fly)	Dis3	NP_001262910.1
Kluyveromyces lactis	KLLA0A10835g	XP_451472.1
Schizosaccharomyces pombe (fission yeast)	dis3	NP_596653.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Use of RNA-seq to quantify the effect of Dis3 knockdown on microRNA expression shows that Dis3 normally regulates a small subset of microRNAs, with only 11 increasing in level >/= 2-fold and 6 decreasing in level >/= 2-fold.
Title: The 3'-5' exoribonuclease Dis3 regulates the expression of specific microRNAs in Drosophila wing imaginal discs.

Dis3 is essential for early Drosophila melanogaster development.
Title: The ribonuclease Dis3 is an essential regulator of the developmental transcriptome.

the dDis3 N-terminus is sufficient for endoribonuclease activity in vitro and that proper N-terminal domain structure is critical for activity of the full-length polypeptide.
Title: Drosophila melanogaster Dis3 N-terminal domains are required for ribonuclease activities, nuclear localization and exosome interactions.

Genome-wide analysis reveals distinct substrate specificities of Dis3, and core exosome subunits
Title: Genome-wide analysis reveals distinct substrate specificities of Rrp6, Dis3, and core exosome subunits.

this study broadens our mechanistic understanding of the general ribonuclease activity of Dis3 and RNR family members.
Title: Characterization of the Drosophila melanogaster Dis3 ribonuclease.

The following Dis3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dis3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05939	NM_142989.4 Latest version!	Drosophila melanogaster Dis3, transcript variant A (Dis3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05939	NM_001275981.1 Latest version!	Drosophila melanogaster Dis3, transcript variant B (Dis3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dis3 ( NM_001275981.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262910.1 Drosophila melanogaster Dis3, transcript variant B (Dis3), mRNA.

Price & Availability↑

CloneID	OFa05939
Clone ID Related Accession (Same CDS sequence)	NM_001275981.1 , NM_142989.4
Accession Version	NM_001275981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dis3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACTT TACGCGAATT TACGCGTAAA ACCAAGCGCG GCAACATTCT GAAGATTGTA 
CGGGAGCACT ATCTGCGCGA CGACATCGGC TGCGGCTCCG AGCTGTGCCG GGAGTGTTTC 
CAAAACGAGG TATACCAGCT CACCTCGCAG CACGCGAATA AGAGCACCCT CTTCAAATTC 
CCGCACTATC TCGTTTTGGA CACAAATGTG GTTCTGGACC AGATCGACGT GCTGGAGGAG 
GATGTTTTGC GCAACGTAGT CGTTCTGACC ACCGTGCTCA ACGAGGTGAA GCACAGGAGC 
TCCGCCATTT ACAAGCGATT CAATGAGATC GTCCACGACC GCACCAGGAA CTTCTATGTG 
TTCGTCAACG AGCACCACAA GGAAACCTAT GCTGATCGCG AACCAGATGA GACCGCCAAT 
GATCGCAATG ATCGGGCCAT TCGTCTGGCC ACCAAGTGGT ACGATGATCA CCTGCAGGCC 
TCGCAATCCT CTAAAGAGTT CGAGCGCAGT GGCAAAGCTG CTACGCGAGT TGTTCTGCTC 
ACAGATGATG CTGCCAATCG TGCTAAGGCC GAAGCAGAAG GTATTCTGGT GACCACAGCA 
GCGGAGTACG TGAAATCTCT CGAGGATTTT CCACTTCTGA TGGATAAGCT GTCGCACAAA 
ACATTCGACA GCGAGAAGAA TGGTCTGCCC CAGTACCCGC CGCATCTCAG CATGAAGGAG 
CTGCTGGAAG GACTGCGCCA AAATAAGCTA CTACAGGGCA CTTTTCAGGC ATCCAGGGAA 
AACTACTTGG AGGGCACCGT CAATGTAGAG AAATTCGAAA AGGGGATCCT TATCCAAGGA 
CGCGAGTCTC TTAATCGGGC CGTGGATGGC GACCTGGTGG CCGTGGAGCT TTTGCCCGAG 
GCGGAGTGGT CAGCACCCAG TGAAATTGTA CTGGAGGAGA AGAATGTCTA TGCCGATGAA 
GTGCCCAGCG AGGAAAGGGC CAAGGATGAA AATGAGATGT TGAATCAGGT GCGTGCCGCT 
GCCTTATCCG CAGAGCGCAC GCCCACAGGG CGAATTGTCG GTATTGTGCG ACGGAAATGG 
CGACAGTATT GCGGCATCCT GCAGCCTAGC TTAATAGAGG ATACAAATCG GCACATCTTT 
GTGCCCGCCG ACCGTAAGAT TCCGCGCATC CGTATCGAAA CGCGACAGGC AGCAATGCTG 
CAGAACCAGC GCATCATCGT AACGATTGAC ACATGGCCGC GCAACTCGCG CTACCCACAT 
GGACATTTTG TGCGCTCCCT GGGACCGCTC GGAGACATGG CCACCGAGAA CGAGGTTATC 
CTGCTGGAGC ACGACGTCCC GCACTGCAAG TTCTCGGACG AAGTGCTTAG CTTCTTGCCG 
AAAATGCCCT GGACAATTAC TGACGAGGAC TATTCCAAGC GTGTGGATCT GAGGGATCTG 
TACATTTGCT CTGTGGATCC CCCGGGCTGC ACGGATATCG ATGATGCTTT GCACTGCAAG 
GAACTACCGA ACGGCAATCT GGAAGTGGGC GTTCACATAG CCGACGTAAG TCACTTTATT 
CGTCCAGGAA ACGCACTGGA CATGGAGGCA GCGGCCAGGG GAACCACCGT GTACTTGGTG 
GGCAAACGAA TCGACATGGT ACCCGAGTTA CTCAGCTCAA ATCTCTGCTC ATTGGTTGGC 
GGTGTTGAGC GATTCGCCTT CTCCTGCGTG TGGGAAGTGG ACAATGAAGC AAATGTGTTA 
AGCAAACGCT TCCACAAGAG CGTCATCAAA TCGAAGCGTG CCATGACCTA CGAGGAAGCG 
CAGAATATCA TCGACGATGC CACGCAACAG AATGAAATAG CCAAGTCCCT GAGAAATCTT 
AATCGGTTGG CCAAAATACT GAAAAAGCGA CGAATGGATA ATGGAGCCCT TGTCCTTGCC 
TCCCCGGAGA TCCGCTTCCA GGTGGACAGC GAGACACACG AACCACTCGA GGTGGAGGTG 
AAGCAGATGC GCGAGACGAA TTCCATGGTG GAGGAGTTCA TGTTGCTGGC CAACATCACG 
GTGGCCGAGC ATATCGCAAC GGAGTTCTCC GAGTGCGCTG TTCTGCGTCG CCATCCTCGG 
CCACCGCCTA CCAATTTCGA TCCGCTGGTA AAGTCTGCGC GCTACCAGGG CTTCCAGGTG 
GACATCAATT CGGGACTGGA GCTGTCGCAT TCACTGGACA AGTGTGTCAA GGCGGATAAT 
CCGTACTTTA ACACAATGAT CCGCATCCTC ACCACACGCT GTATGATGCA GGCAGTGTAC 
TTCATCAGCG GCAGTCTGCA GAAGGAGGAG TTCTTCCACT ACGGTCTGGC TGCTCCCATC 
TACACGCACT TTACGTCGCC CATTCGTCGT TACTCCGACA TTATGGTACA TCGCCTGCTG 
GCCGCCTCAA TCGGCGCGGA TAGCACCTAT GCCCAGCTAT TGGACCGAAA GTCAAATGAG 
GAGCTGTGTC ATAATCTTAA CTACCGCCAC AAAATGGCTC AGTACGCTGG TCGCGCATCA 
GTGGCCCTCA ACACGCATCT CTTTTTCCGC GGCAAGGAGG AGGATGAGGA AGGATATGTG 
CTATTTGTGC GCAAGAATGC TCTGCAAGTG CTAATCCCCA AGTATGGCCT GGAAGGCACT 
TTGTATCTAA AGTCGGACAA GGATGGCAAG GACGGTGTGG AGCGCGTCAA GAGCGAGATT 
GTCTTCACCT TCAACGAAGA GGATCATACA CAGCGTTGCG GTGACGTGGT GTTCCACTCC 
TTTGATCCAG TCACGGTTCG TCTTAGCCTG GATTCCAGCA ATGTGCAGCA CGAGAAGCTG 
ATCTTCCGGC TGGTCAAGCC GTACATCAAG GGCTTCAGCG TGGAAACGAC CACCGAAGGA 
ACTGATAGCC AAGCGGTTTC TGCGGCACCT CCATCGAAAA AGACAAAGAA GGATAAGAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262910.1
CDS	61..3009
Translation	MQTLREFTRKTKRGNILKIVREHYLRDDIGCGSELCRECFQNEVYQLTSQHANKSTLFKFPHYLVLDTNVVLDQIDVLEEDVLRNVVVLTTVLNEVKHRSSAIYKRFNEIVHDRTRNFYVFVNEHHKETYADREPDETANDRNDRAIRLATKWYDDHLQASQSSKEFERSGKAATRVVLLTDDAANRAKAEAEGILVTTAAEYVKSLEDFPLLMDKLSHKTFDSEKNGLPQYPPHLSMKELLEGLRQNKLLQGTFQASRENYLEGTVNVEKFEKGILIQGRESLNRAVDGDLVAVELLPEAEWSAPSEIVLEEKNVYADEVPSEERAKDENEMLNQVRAAALSAERTPTGRIVGIVRRKWRQYCGILQPSLIEDTNRHIFVPADRKIPRIRIETRQAAMLQNQRIIVTIDTWPRNSRYPHGHFVRSLGPLGDMATENEVILLEHDVPHCKFSDEVLSFLPKMPWTITDEDYSKRVDLRDLYICSVDPPGCTDIDDALHCKELPNGNLEVGVHIADVSHFIRPGNALDMEAAARGTTVYLVGKRIDMVPELLSSNLCSLVGGVERFAFSCVWEVDNEANVLSKRFHKSVIKSKRAMTYEEAQNIIDDATQQNEIAKSLRNLNRLAKILKKRRMDNGALVLASPEIRFQVDSETHEPLEVEVKQMRETNSMVEEFMLLANITVAEHIATEFSECAVLRRHPRPPPTNFDPLVKSARYQGFQVDINSGLELSHSLDKCVKADNPYFNTMIRILTTRCMMQAVYFISGSLQKEEFFHYGLAAPIYTHFTSPIRRYSDIMVHRLLAASIGADSTYAQLLDRKSNEELCHNLNYRHKMAQYAGRASVALNTHLFFRGKEEDEEGYVLFVRKNALQVLIPKYGLEGTLYLKSDKDGKDGVERVKSEIVFTFNEEDHTQRCGDVVFHSFDPVTVRLSLDSSNVQHEKLIFRLVKPYIKGFSVETTTEGTDSQAVSAAPPSKKTKKDKKKK

Target ORF information:

RefSeq Version	NM_001275981.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dis3, transcript variant B (Dis3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275981.1

ORF Insert Sequence:

ATGCAAACTT TACGCGAATT TACGCGTAAA ACCAAGCGCG GCAACATTCT GAAGATTGTA 
CGGGAGCACT ATCTGCGCGA CGACATCGGC TGCGGCTCCG AGCTGTGCCG GGAGTGTTTC 
CAAAACGAGG TATACCAGCT CACCTCGCAG CACGCGAATA AGAGCACCCT CTTCAAATTC 
CCGCACTATC TCGTTTTGGA CACAAATGTG GTTCTGGACC AGATCGACGT GCTGGAGGAG 
GATGTTTTGC GCAACGTAGT CGTTCTGACC ACCGTGCTCA ACGAGGTGAA GCACAGGAGC 
TCCGCCATTT ACAAGCGATT CAATGAGATC GTCCACGACC GCACCAGGAA CTTCTATGTG 
TTCGTCAACG AGCACCACAA GGAAACCTAT GCTGATCGCG AACCAGATGA GACCGCCAAT 
GATCGCAATG ATCGGGCCAT TCGTCTGGCC ACCAAGTGGT ACGATGATCA CCTGCAGGCC 
TCGCAATCCT CTAAAGAGTT CGAGCGCAGT GGCAAAGCTG CTACGCGAGT TGTTCTGCTC 
ACAGATGATG CTGCCAATCG TGCTAAGGCC GAAGCAGAAG GTATTCTGGT GACCACAGCA 
GCGGAGTACG TGAAATCTCT CGAGGATTTT CCACTTCTGA TGGATAAGCT GTCGCACAAA 
ACATTCGACA GCGAGAAGAA TGGTCTGCCC CAGTACCCGC CGCATCTCAG CATGAAGGAG 
CTGCTGGAAG GACTGCGCCA AAATAAGCTA CTACAGGGCA CTTTTCAGGC ATCCAGGGAA 
AACTACTTGG AGGGCACCGT CAATGTAGAG AAATTCGAAA AGGGGATCCT TATCCAAGGA 
CGCGAGTCTC TTAATCGGGC CGTGGATGGC GACCTGGTGG CCGTGGAGCT TTTGCCCGAG 
GCGGAGTGGT CAGCACCCAG TGAAATTGTA CTGGAGGAGA AGAATGTCTA TGCCGATGAA 
GTGCCCAGCG AGGAAAGGGC CAAGGATGAA AATGAGATGT TGAATCAGGT GCGTGCCGCT 
GCCTTATCCG CAGAGCGCAC GCCCACAGGG CGAATTGTCG GTATTGTGCG ACGGAAATGG 
CGACAGTATT GCGGCATCCT GCAGCCTAGC TTAATAGAGG ATACAAATCG GCACATCTTT 
GTGCCCGCCG ACCGTAAGAT TCCGCGCATC CGTATCGAAA CGCGACAGGC AGCAATGCTG 
CAGAACCAGC GCATCATCGT AACGATTGAC ACATGGCCGC GCAACTCGCG CTACCCACAT 
GGACATTTTG TGCGCTCCCT GGGACCGCTC GGAGACATGG CCACCGAGAA CGAGGTTATC 
CTGCTGGAGC ACGACGTCCC GCACTGCAAG TTCTCGGACG AAGTGCTTAG CTTCTTGCCG 
AAAATGCCCT GGACAATTAC TGACGAGGAC TATTCCAAGC GTGTGGATCT GAGGGATCTG 
TACATTTGCT CTGTGGATCC CCCGGGCTGC ACGGATATCG ATGATGCTTT GCACTGCAAG 
GAACTACCGA ACGGCAATCT GGAAGTGGGC GTTCACATAG CCGACGTAAG TCACTTTATT 
CGTCCAGGAA ACGCACTGGA CATGGAGGCA GCGGCCAGGG GAACCACCGT GTACTTGGTG 
GGCAAACGAA TCGACATGGT ACCCGAGTTA CTCAGCTCAA ATCTCTGCTC ATTGGTTGGC 
GGTGTTGAGC GATTCGCCTT CTCCTGCGTG TGGGAAGTGG ACAATGAAGC AAATGTGTTA 
AGCAAACGCT TCCACAAGAG CGTCATCAAA TCGAAGCGTG CCATGACCTA CGAGGAAGCG 
CAGAATATCA TCGACGATGC CACGCAACAG AATGAAATAG CCAAGTCCCT GAGAAATCTT 
AATCGGTTGG CCAAAATACT GAAAAAGCGA CGAATGGATA ATGGAGCCCT TGTCCTTGCC 
TCCCCGGAGA TCCGCTTCCA GGTGGACAGC GAGACACACG AACCACTCGA GGTGGAGGTG 
AAGCAGATGC GCGAGACGAA TTCCATGGTG GAGGAGTTCA TGTTGCTGGC CAACATCACG 
GTGGCCGAGC ATATCGCAAC GGAGTTCTCC GAGTGCGCTG TTCTGCGTCG CCATCCTCGG 
CCACCGCCTA CCAATTTCGA TCCGCTGGTA AAGTCTGCGC GCTACCAGGG CTTCCAGGTG 
GACATCAATT CGGGACTGGA GCTGTCGCAT TCACTGGACA AGTGTGTCAA GGCGGATAAT 
CCGTACTTTA ACACAATGAT CCGCATCCTC ACCACACGCT GTATGATGCA GGCAGTGTAC 
TTCATCAGCG GCAGTCTGCA GAAGGAGGAG TTCTTCCACT ACGGTCTGGC TGCTCCCATC 
TACACGCACT TTACGTCGCC CATTCGTCGT TACTCCGACA TTATGGTACA TCGCCTGCTG 
GCCGCCTCAA TCGGCGCGGA TAGCACCTAT GCCCAGCTAT TGGACCGAAA GTCAAATGAG 
GAGCTGTGTC ATAATCTTAA CTACCGCCAC AAAATGGCTC AGTACGCTGG TCGCGCATCA 
GTGGCCCTCA ACACGCATCT CTTTTTCCGC GGCAAGGAGG AGGATGAGGA AGGATATGTG 
CTATTTGTGC GCAAGAATGC TCTGCAAGTG CTAATCCCCA AGTATGGCCT GGAAGGCACT 
TTGTATCTAA AGTCGGACAA GGATGGCAAG GACGGTGTGG AGCGCGTCAA GAGCGAGATT 
GTCTTCACCT TCAACGAAGA GGATCATACA CAGCGTTGCG GTGACGTGGT GTTCCACTCC 
TTTGATCCAG TCACGGTTCG TCTTAGCCTG GATTCCAGCA ATGTGCAGCA CGAGAAGCTG 
ATCTTCCGGC TGGTCAAGCC GTACATCAAG GGCTTCAGCG TGGAAACGAC CACCGAAGGA 
ACTGATAGCC AAGCGGTTTC TGCGGCACCT CCATCGAAAA AGACAAAGAA GGATAAGAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dis3 ( NM_142989.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651246.2 Drosophila melanogaster Dis3, transcript variant A (Dis3), mRNA.

Price & Availability↑

CloneID	OFa05939
Clone ID Related Accession (Same CDS sequence)	NM_001275981.1 , NM_142989.4
Accession Version	NM_142989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dis3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142989.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACTT TACGCGAATT TACGCGTAAA ACCAAGCGCG GCAACATTCT GAAGATTGTA 
CGGGAGCACT ATCTGCGCGA CGACATCGGC TGCGGCTCCG AGCTGTGCCG GGAGTGTTTC 
CAAAACGAGG TATACCAGCT CACCTCGCAG CACGCGAATA AGAGCACCCT CTTCAAATTC 
CCGCACTATC TCGTTTTGGA CACAAATGTG GTTCTGGACC AGATCGACGT GCTGGAGGAG 
GATGTTTTGC GCAACGTAGT CGTTCTGACC ACCGTGCTCA ACGAGGTGAA GCACAGGAGC 
TCCGCCATTT ACAAGCGATT CAATGAGATC GTCCACGACC GCACCAGGAA CTTCTATGTG 
TTCGTCAACG AGCACCACAA GGAAACCTAT GCTGATCGCG AACCAGATGA GACCGCCAAT 
GATCGCAATG ATCGGGCCAT TCGTCTGGCC ACCAAGTGGT ACGATGATCA CCTGCAGGCC 
TCGCAATCCT CTAAAGAGTT CGAGCGCAGT GGCAAAGCTG CTACGCGAGT TGTTCTGCTC 
ACAGATGATG CTGCCAATCG TGCTAAGGCC GAAGCAGAAG GTATTCTGGT GACCACAGCA 
GCGGAGTACG TGAAATCTCT CGAGGATTTT CCACTTCTGA TGGATAAGCT GTCGCACAAA 
ACATTCGACA GCGAGAAGAA TGGTCTGCCC CAGTACCCGC CGCATCTCAG CATGAAGGAG 
CTGCTGGAAG GACTGCGCCA AAATAAGCTA CTACAGGGCA CTTTTCAGGC ATCCAGGGAA 
AACTACTTGG AGGGCACCGT CAATGTAGAG AAATTCGAAA AGGGGATCCT TATCCAAGGA 
CGCGAGTCTC TTAATCGGGC CGTGGATGGC GACCTGGTGG CCGTGGAGCT TTTGCCCGAG 
GCGGAGTGGT CAGCACCCAG TGAAATTGTA CTGGAGGAGA AGAATGTCTA TGCCGATGAA 
GTGCCCAGCG AGGAAAGGGC CAAGGATGAA AATGAGATGT TGAATCAGGT GCGTGCCGCT 
GCCTTATCCG CAGAGCGCAC GCCCACAGGG CGAATTGTCG GTATTGTGCG ACGGAAATGG 
CGACAGTATT GCGGCATCCT GCAGCCTAGC TTAATAGAGG ATACAAATCG GCACATCTTT 
GTGCCCGCCG ACCGTAAGAT TCCGCGCATC CGTATCGAAA CGCGACAGGC AGCAATGCTG 
CAGAACCAGC GCATCATCGT AACGATTGAC ACATGGCCGC GCAACTCGCG CTACCCACAT 
GGACATTTTG TGCGCTCCCT GGGACCGCTC GGAGACATGG CCACCGAGAA CGAGGTTATC 
CTGCTGGAGC ACGACGTCCC GCACTGCAAG TTCTCGGACG AAGTGCTTAG CTTCTTGCCG 
AAAATGCCCT GGACAATTAC TGACGAGGAC TATTCCAAGC GTGTGGATCT GAGGGATCTG 
TACATTTGCT CTGTGGATCC CCCGGGCTGC ACGGATATCG ATGATGCTTT GCACTGCAAG 
GAACTACCGA ACGGCAATCT GGAAGTGGGC GTTCACATAG CCGACGTAAG TCACTTTATT 
CGTCCAGGAA ACGCACTGGA CATGGAGGCA GCGGCCAGGG GAACCACCGT GTACTTGGTG 
GGCAAACGAA TCGACATGGT ACCCGAGTTA CTCAGCTCAA ATCTCTGCTC ATTGGTTGGC 
GGTGTTGAGC GATTCGCCTT CTCCTGCGTG TGGGAAGTGG ACAATGAAGC AAATGTGTTA 
AGCAAACGCT TCCACAAGAG CGTCATCAAA TCGAAGCGTG CCATGACCTA CGAGGAAGCG 
CAGAATATCA TCGACGATGC CACGCAACAG AATGAAATAG CCAAGTCCCT GAGAAATCTT 
AATCGGTTGG CCAAAATACT GAAAAAGCGA CGAATGGATA ATGGAGCCCT TGTCCTTGCC 
TCCCCGGAGA TCCGCTTCCA GGTGGACAGC GAGACACACG AACCACTCGA GGTGGAGGTG 
AAGCAGATGC GCGAGACGAA TTCCATGGTG GAGGAGTTCA TGTTGCTGGC CAACATCACG 
GTGGCCGAGC ATATCGCAAC GGAGTTCTCC GAGTGCGCTG TTCTGCGTCG CCATCCTCGG 
CCACCGCCTA CCAATTTCGA TCCGCTGGTA AAGTCTGCGC GCTACCAGGG CTTCCAGGTG 
GACATCAATT CGGGACTGGA GCTGTCGCAT TCACTGGACA AGTGTGTCAA GGCGGATAAT 
CCGTACTTTA ACACAATGAT CCGCATCCTC ACCACACGCT GTATGATGCA GGCAGTGTAC 
TTCATCAGCG GCAGTCTGCA GAAGGAGGAG TTCTTCCACT ACGGTCTGGC TGCTCCCATC 
TACACGCACT TTACGTCGCC CATTCGTCGT TACTCCGACA TTATGGTACA TCGCCTGCTG 
GCCGCCTCAA TCGGCGCGGA TAGCACCTAT GCCCAGCTAT TGGACCGAAA GTCAAATGAG 
GAGCTGTGTC ATAATCTTAA CTACCGCCAC AAAATGGCTC AGTACGCTGG TCGCGCATCA 
GTGGCCCTCA ACACGCATCT CTTTTTCCGC GGCAAGGAGG AGGATGAGGA AGGATATGTG 
CTATTTGTGC GCAAGAATGC TCTGCAAGTG CTAATCCCCA AGTATGGCCT GGAAGGCACT 
TTGTATCTAA AGTCGGACAA GGATGGCAAG GACGGTGTGG AGCGCGTCAA GAGCGAGATT 
GTCTTCACCT TCAACGAAGA GGATCATACA CAGCGTTGCG GTGACGTGGT GTTCCACTCC 
TTTGATCCAG TCACGGTTCG TCTTAGCCTG GATTCCAGCA ATGTGCAGCA CGAGAAGCTG 
ATCTTCCGGC TGGTCAAGCC GTACATCAAG GGCTTCAGCG TGGAAACGAC CACCGAAGGA 
ACTGATAGCC AAGCGGTTTC TGCGGCACCT CCATCGAAAA AGACAAAGAA GGATAAGAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651246.2
CDS	61..3009
Translation	MQTLREFTRKTKRGNILKIVREHYLRDDIGCGSELCRECFQNEVYQLTSQHANKSTLFKFPHYLVLDTNVVLDQIDVLEEDVLRNVVVLTTVLNEVKHRSSAIYKRFNEIVHDRTRNFYVFVNEHHKETYADREPDETANDRNDRAIRLATKWYDDHLQASQSSKEFERSGKAATRVVLLTDDAANRAKAEAEGILVTTAAEYVKSLEDFPLLMDKLSHKTFDSEKNGLPQYPPHLSMKELLEGLRQNKLLQGTFQASRENYLEGTVNVEKFEKGILIQGRESLNRAVDGDLVAVELLPEAEWSAPSEIVLEEKNVYADEVPSEERAKDENEMLNQVRAAALSAERTPTGRIVGIVRRKWRQYCGILQPSLIEDTNRHIFVPADRKIPRIRIETRQAAMLQNQRIIVTIDTWPRNSRYPHGHFVRSLGPLGDMATENEVILLEHDVPHCKFSDEVLSFLPKMPWTITDEDYSKRVDLRDLYICSVDPPGCTDIDDALHCKELPNGNLEVGVHIADVSHFIRPGNALDMEAAARGTTVYLVGKRIDMVPELLSSNLCSLVGGVERFAFSCVWEVDNEANVLSKRFHKSVIKSKRAMTYEEAQNIIDDATQQNEIAKSLRNLNRLAKILKKRRMDNGALVLASPEIRFQVDSETHEPLEVEVKQMRETNSMVEEFMLLANITVAEHIATEFSECAVLRRHPRPPPTNFDPLVKSARYQGFQVDINSGLELSHSLDKCVKADNPYFNTMIRILTTRCMMQAVYFISGSLQKEEFFHYGLAAPIYTHFTSPIRRYSDIMVHRLLAASIGADSTYAQLLDRKSNEELCHNLNYRHKMAQYAGRASVALNTHLFFRGKEEDEEGYVLFVRKNALQVLIPKYGLEGTLYLKSDKDGKDGVERVKSEIVFTFNEEDHTQRCGDVVFHSFDPVTVRLSLDSSNVQHEKLIFRLVKPYIKGFSVETTTEGTDSQAVSAAPPSKKTKKDKKKK

Target ORF information:

RefSeq Version	NM_142989.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dis3, transcript variant A (Dis3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142989.4

ORF Insert Sequence:

ATGCAAACTT TACGCGAATT TACGCGTAAA ACCAAGCGCG GCAACATTCT GAAGATTGTA 
CGGGAGCACT ATCTGCGCGA CGACATCGGC TGCGGCTCCG AGCTGTGCCG GGAGTGTTTC 
CAAAACGAGG TATACCAGCT CACCTCGCAG CACGCGAATA AGAGCACCCT CTTCAAATTC 
CCGCACTATC TCGTTTTGGA CACAAATGTG GTTCTGGACC AGATCGACGT GCTGGAGGAG 
GATGTTTTGC GCAACGTAGT CGTTCTGACC ACCGTGCTCA ACGAGGTGAA GCACAGGAGC 
TCCGCCATTT ACAAGCGATT CAATGAGATC GTCCACGACC GCACCAGGAA CTTCTATGTG 
TTCGTCAACG AGCACCACAA GGAAACCTAT GCTGATCGCG AACCAGATGA GACCGCCAAT 
GATCGCAATG ATCGGGCCAT TCGTCTGGCC ACCAAGTGGT ACGATGATCA CCTGCAGGCC 
TCGCAATCCT CTAAAGAGTT CGAGCGCAGT GGCAAAGCTG CTACGCGAGT TGTTCTGCTC 
ACAGATGATG CTGCCAATCG TGCTAAGGCC GAAGCAGAAG GTATTCTGGT GACCACAGCA 
GCGGAGTACG TGAAATCTCT CGAGGATTTT CCACTTCTGA TGGATAAGCT GTCGCACAAA 
ACATTCGACA GCGAGAAGAA TGGTCTGCCC CAGTACCCGC CGCATCTCAG CATGAAGGAG 
CTGCTGGAAG GACTGCGCCA AAATAAGCTA CTACAGGGCA CTTTTCAGGC ATCCAGGGAA 
AACTACTTGG AGGGCACCGT CAATGTAGAG AAATTCGAAA AGGGGATCCT TATCCAAGGA 
CGCGAGTCTC TTAATCGGGC CGTGGATGGC GACCTGGTGG CCGTGGAGCT TTTGCCCGAG 
GCGGAGTGGT CAGCACCCAG TGAAATTGTA CTGGAGGAGA AGAATGTCTA TGCCGATGAA 
GTGCCCAGCG AGGAAAGGGC CAAGGATGAA AATGAGATGT TGAATCAGGT GCGTGCCGCT 
GCCTTATCCG CAGAGCGCAC GCCCACAGGG CGAATTGTCG GTATTGTGCG ACGGAAATGG 
CGACAGTATT GCGGCATCCT GCAGCCTAGC TTAATAGAGG ATACAAATCG GCACATCTTT 
GTGCCCGCCG ACCGTAAGAT TCCGCGCATC CGTATCGAAA CGCGACAGGC AGCAATGCTG 
CAGAACCAGC GCATCATCGT AACGATTGAC ACATGGCCGC GCAACTCGCG CTACCCACAT 
GGACATTTTG TGCGCTCCCT GGGACCGCTC GGAGACATGG CCACCGAGAA CGAGGTTATC 
CTGCTGGAGC ACGACGTCCC GCACTGCAAG TTCTCGGACG AAGTGCTTAG CTTCTTGCCG 
AAAATGCCCT GGACAATTAC TGACGAGGAC TATTCCAAGC GTGTGGATCT GAGGGATCTG 
TACATTTGCT CTGTGGATCC CCCGGGCTGC ACGGATATCG ATGATGCTTT GCACTGCAAG 
GAACTACCGA ACGGCAATCT GGAAGTGGGC GTTCACATAG CCGACGTAAG TCACTTTATT 
CGTCCAGGAA ACGCACTGGA CATGGAGGCA GCGGCCAGGG GAACCACCGT GTACTTGGTG 
GGCAAACGAA TCGACATGGT ACCCGAGTTA CTCAGCTCAA ATCTCTGCTC ATTGGTTGGC 
GGTGTTGAGC GATTCGCCTT CTCCTGCGTG TGGGAAGTGG ACAATGAAGC AAATGTGTTA 
AGCAAACGCT TCCACAAGAG CGTCATCAAA TCGAAGCGTG CCATGACCTA CGAGGAAGCG 
CAGAATATCA TCGACGATGC CACGCAACAG AATGAAATAG CCAAGTCCCT GAGAAATCTT 
AATCGGTTGG CCAAAATACT GAAAAAGCGA CGAATGGATA ATGGAGCCCT TGTCCTTGCC 
TCCCCGGAGA TCCGCTTCCA GGTGGACAGC GAGACACACG AACCACTCGA GGTGGAGGTG 
AAGCAGATGC GCGAGACGAA TTCCATGGTG GAGGAGTTCA TGTTGCTGGC CAACATCACG 
GTGGCCGAGC ATATCGCAAC GGAGTTCTCC GAGTGCGCTG TTCTGCGTCG CCATCCTCGG 
CCACCGCCTA CCAATTTCGA TCCGCTGGTA AAGTCTGCGC GCTACCAGGG CTTCCAGGTG 
GACATCAATT CGGGACTGGA GCTGTCGCAT TCACTGGACA AGTGTGTCAA GGCGGATAAT 
CCGTACTTTA ACACAATGAT CCGCATCCTC ACCACACGCT GTATGATGCA GGCAGTGTAC 
TTCATCAGCG GCAGTCTGCA GAAGGAGGAG TTCTTCCACT ACGGTCTGGC TGCTCCCATC 
TACACGCACT TTACGTCGCC CATTCGTCGT TACTCCGACA TTATGGTACA TCGCCTGCTG 
GCCGCCTCAA TCGGCGCGGA TAGCACCTAT GCCCAGCTAT TGGACCGAAA GTCAAATGAG 
GAGCTGTGTC ATAATCTTAA CTACCGCCAC AAAATGGCTC AGTACGCTGG TCGCGCATCA 
GTGGCCCTCA ACACGCATCT CTTTTTCCGC GGCAAGGAGG AGGATGAGGA AGGATATGTG 
CTATTTGTGC GCAAGAATGC TCTGCAAGTG CTAATCCCCA AGTATGGCCT GGAAGGCACT 
TTGTATCTAA AGTCGGACAA GGATGGCAAG GACGGTGTGG AGCGCGTCAA GAGCGAGATT 
GTCTTCACCT TCAACGAAGA GGATCATACA CAGCGTTGCG GTGACGTGGT GTTCCACTCC 
TTTGATCCAG TCACGGTTCG TCTTAGCCTG GATTCCAGCA ATGTGCAGCA CGAGAAGCTG 
ATCTTCCGGC TGGTCAAGCC GTACATCAAG GGCTTCAGCG TGGAAACGAC CACCGAAGGA 
ACTGATAGCC AAGCGGTTTC TGCGGCACCT CCATCGAAAA AGACAAAGAA GGATAAGAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dis3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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