Gene Symbol | DOA4 |
Entrez Gene ID | 851641 |
Full Name | ubiquitin-specific protease DOA4 |
Synonyms | DOS1,MUT4,NPI2,SSV7,UBP4 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » DOA4 cDNA ORF clone
Gene Symbol | DOA4 |
Entrez Gene ID | 851641 |
Full Name | ubiquitin-specific protease DOA4 |
Synonyms | DOS1,MUT4,NPI2,SSV7,UBP4 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001180377.3 | NP_010354.3 | ubiquitin-specific protease DOA4 |
Kluyveromyces lactis | KLLA0E10275g | XP_454413.1 |
Eremothecium gossypii | AGOS_AFR007W | NP_985554.2 |
Saccharomyces cerevisiae (baker's yeast) | DOA4 | NP_010354.3 |
The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Doa4 physically interacts with the ESCRT-III component Snf7 and preferentially cleaves Lys63-linked ubiquitin oligomers involved in membrane protein trafficking
Title: The N-terminal domains determine cellular localization and functions of the Doa4 and Ubp5 deubiquitinating enzymes.
Binding of Vps20 to the Doa4 N-terminus inhibits a non-catalytic function of Doa4 that promotes ILV formation.
Title: Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III.
identified a protein designated Rfu1 (regulator of free ubiquitin chains 1), which regulates intracellular concentrations of monomeric ubiquitins; functions as an inhibitor of Doa4
Title: An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis.
Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies.
Title: Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies.
Deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body (MVB) sorting machinery is essential to correct sorting of cargoes into the MVB pathway.
Title: Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway.
The following DOA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi01158 | |
Clone ID Related Accession (Same CDS sequence) | NM_001180377.3 | |
Accession Version | NM_001180377.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | ubiquitin-specific protease DOA4 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180377.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGGAGCAGA ATATTATTAG TACCATAAGG GATGAGTGTA TTCGTCACCG GTCGAAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_010354.3 |
CDS | 1..2781 |
Translation |
Target ORF information:
Target ORF information:
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1 | ATGGAGCAGA ATATTATTAG TACCATAAGG GATGAGTGTA TTCGTCACCG GTCGAAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome IV. |
Life with 6000 genes. |