brr2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	brr2
Entrez Gene ID	2543676
Full Name	U5 snRNP complex subunit Brr2 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018778.2	NP_593346.1	putative U5 snRNP complex subunit Brr2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	brr2	NP_593346.1
Canis lupus familiaris (dog)	SNRNP200	XP_532949.2
Bos taurus (cattle)	SNRNP200	NP_001193092.1
Caenorhabditis elegans (roundworm)	snrp-200	NP_496710.1
Kluyveromyces lactis	KLLA0C17798g	XP_452995.1
Arabidopsis thaliana (thale cress)	AT2G42270	NP_181756.1
Pan troglodytes (chimpanzee)	SNRNP200	XP_001147672.1
Saccharomyces cerevisiae (baker's yeast)	BRR2	NP_011099.1
Eremothecium gossypii	AGOS_AGR113W	NP_986779.1
Neurospora crassa	NCU02685	XP_965458.1
Xenopus tropicalis (tropical clawed frog)	snrnp200	XP_002932581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001519	XP_321605.3
Magnaporthe oryzae (rice blast fungus)	MGG_15536	XP_003713506.1
Homo sapiens (human)	SNRNP200	NP_054733.2
	SNRNP200	XP_001098299.2
Mus musculus (house mouse)	Snrnp200	NP_796188.2
Rattus norvegicus (Norway rat)	Snrnp200	NP_001032855.2
Danio rerio (zebrafish)	snrnp200	NP_001116729.1
Drosophila melanogaster (fruit fly)	l(3)72Ab	NP_648818.3
Arabidopsis thaliana (thale cress)	emb1507	NP_001185050.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prp1 and Brr2 are complexed in vivo with spliceosomal particles containing the five snRNAs U1, U2, U5, and base-paired U4/U6
Title: Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe.

The following brr2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brr2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103134	NM_001018778.2 Latest version!	Schizosaccharomyces pombe putative U5 snRNP complex subunit Brr2 (brr2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brr2 ( NM_001018778.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593346.1 Schizosaccharomyces pombe putative U5 snRNP complex subunit Brr2 (brr2), mRNA.

Price & Availability↑

CloneID	OSc103134
Clone ID Related Accession (Same CDS sequence)	NM_001018778.2
Accession Version	NM_001018778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative U5 snRNP complex subunit Brr2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018778.1.

ATGTCGAGTG CACATCCTAA AGGGGACTCG AAAGAGCCTC CCAAACATGG AAATAGTAAG 
GAGAAGCCAA ACTATGGTCA ATCACAGTAT AGTTATTCTG CAATGTCAAA TTTGGTTACG 
CAGGCTGATC GAAGATTTGT GTCACGACGT GATGCTGAAC CTACAGGCGA ACCGGAATCT 
CTGGTAAATC GAGTTAGTAT TGCAGATATG GGTTCTAGAG CGAGAATTGA AAAACCTAGT 
ACCCTTCCTC TCGAATTGAC GCAGGAAGTT CAGGAGGTCC GGTTACCTCG TAAAGATGCA 
GAATCCTTAG AAATTGGTAT ACGGCAACCT GAACGCGAAA AGCGCTCTTC AGCGATACTT 
AAATATTTTG ATTCTTTTGA AATTTTGAAA TATAATCCTC TAACCGACGA GACCCGAGAA 
GTTTATGATT ATATCTTGAG TTTCATTCAA CAATACCTTG GCGATCAGTC TCCTGAAATC 
CTCCGTTCTG CTGCTGATTT GATTATTGAA CTTCTTAAGG ATAGTTCTTT AGATGAACAA 
GGTCGTAAAA AGCAAATAGA GGAGGTTTTG AGTACTGAAC TCCCACAGGA TCGGTTTTCA 
CAGCTCGTTA ACTTGGGAAA TCGCTTAACA GATTATACAG TTGAGCAAGA AGAAGAGTTA 
AATGAAGAAG GCGTTAATGA ATCTGGCGTA CCAGTATTGT TCAATGAAGC CGATGAAGAG 
GAAGAAGCTG TTGAGGCAAT GGAAGAGGAT GAAGTGGCAG AAGATGAAGA CGTCGTCTTA 
GAAACTTCCA TATCTCAAGA AGAAGAGAAA AAGAATATTG AAAATCCGGA TACAGAGGTT 
ACATTTATTT CTGCTGATAC CAAAAAAGTT ACCGAAATAC CCACTGTTCA TCCTCGTGAA 
ATCGATGCAT TTTGGCTGCA ACGAGAAATA GCAAAATATT TTGCTGACGC CGTCGTTTGT 
CAGGAAAAAA CAAATCAAGC GTTTGAGGCA CTTTCGGCTG ATTACGATCT CGGTGAATTG 
GAAAATGAAT TGATGAGTAT TTTTGATTAT GAACATTTCT ATCTTGTTCA ACTTTTGACT 
AAGAATCGTT GGACAATAGT TTCGTGTACA ATGTTAAAAC GCGCCGCTAC TGATGAAGAA 
CGCTTAGGTG TGGAAGAGCA AATTCGTGCT GCTGGTCGGT CTTGGATTCT CGAAGCTCTA 
CGCCCTGGTG CTATCACTAT ACCTGATGAC GGTTTAAATG AATTAAATAA TAATGTTGTT 
GAGAAAGCTG AACCTGCGCC CGTTTCTGAA ATTCCACTTT CGAAAACTTT GACTTCCCAT 
AAAATTGTAC CTAAGCACCA AGTTGATCTA GAAAATTATG TGTTTACTGA GGGATCACGT 
CTCATGTCTA ATAAAGCTGT CAAGCTACCT GAAGGCTCGT TTCGTAGGAC TGGTAAAGGG 
TATGAAGAAA TACATGTTCC TGCCCCCAAC AAAGCCGTAC TTGGCGCTGA CGAGCGTCTT 
GTAAAAATCA AGGAACTGCC TGAATGGTCT CATCAAGCTT TTCTTAATAC TCAATCTTTA 
AACCGAATCC AAAGCCATCT TTATCCTATC GCTTTTGGTA CTGACGAAAA CATCCTTTTA 
TGCGCTCCTA CTGGTGCTGG AAAAACAAAT GTAGCAATGC TATGTATCCT TAACGAATTA 
CAAAAACATT TACGTGAAGA TCTTAGCTTT AACTTACAAA ATTTTAAAAT TGTTTACATT 
GCTCCCTTGA AGGCGTTGGT ACAAGAGATG GTTAATAATT TCTCAAAGAG GCTTACTCCA 
TACAATATTC GAGTCGCAGA GTTAACTGGT GATAGTCAAT TGACTAAACA ACAAATTAGT 
GAAACTCAAA TAATTGTAAC CACACCCGAG AAGTGGGATA TTATAACTCG AAAAGCTAAT 
GATTTATCTT ATGTCAATCT AGTCCGTTTA GTTATTATTG ATGAGGTTCA TTTGCTTCAT 
GATGAACGAG GTCCTGTATT AGAATCTATA GTTGCTCGTA TATTTCGCCA TCAGGAGGAA 
ACTCTTGAAC AAGTTCGATT AGTAGGTTTG TCTGCTACAC TTCCTAATTA TACCGATGTT 
GCCAGTTTTT TACATGTCGA CCCTAAAAAA GGGCTTTTTT ACTTCGATTC GACATATCGT 
CCATGCCCTT TAAAGCAAGA ATTTATAGGC ATTACAGAAA AAACACCGTT TAAACGAATG 
CAGACGACTA ATGAAGCTTG TTATGAAAAA GTTATGCAAC ACGCTGGCAA AAACCAGGTC 
CTCATATTTG TACATTCGCG CAAAGAAACC GCTAAAACCG CTCGTTTTAT AAGAGATAAG 
GCACTAGAGG AAGAGACCAT CGGTCATTTG TTACGCTCTG ATGCCGCATC CCGTGAAATT 
CTTCGGGCTG AAGCCGACTC TACCAGTGAT GAAAATTTGA AAGATTTGCT CCCATATGGG 
TTTGCCATAC ATCATGCTGG TATGCGACGA GAGGATCGCC AAACGTCTGA AGACCTTTTT 
GCCGATGGTA CTATACAGGT ACTAGTGAGT ACGGCAACTC TGGCTTGGGG CGTAAACTTA 
CCAGCGCACA CAGTGATCAT CAAAGGGACC CAAGTTTATT CTCCTGAAAA GGGTATCTGG 
ACAGAACTCT CTCCTCAGGA TGTTTTGCAA ATGCTTGGCA GAGCTGGTCG CCCACAATTC 
GACACATATG GTGAAGGAAT TATCATTACC GCCCACTCTG AGTTACAGTA TTATCTATCA 
CTAATGAATC AGCAGCTTCC AATTGAGTCT CAATTTATGC GCCGTCTAGC TGATTGTTTA 
AATGCCGAAG TATCGTTAGG AACTGTTCGT AGTATCGAAG ATGGCGTAGA CTGGCTTGGT 
TATACATACC TCTATGTTCG AATGTTACGA TCCCCTGCCC TATATAGCGT CGGCCCAGAG 
TATGATGATG ACAAATATCT TGTTCAAAAA AGAGCTGACT TACTTCATTC AGCTGCCATT 
TTATTAGAAA AATGCAAGTT ACTAGTGTAT AATCGTCAGA GTGGTACGTT GACCGCTACG 
GAATTGGGAA AAGTTGCTGC AAGTTATTAC GTTACTCATA ACTCGATGGC CATATATAAT 
CGTCTTTTAA TGCAGACAAC ATCCTTTATC GAGTTATTTC GAGTCTTCAG TTTTTCTGAT 
GAATTTAAGC ACATTCCTGT CCGTGAAGAA GAGAAAGTTG AACTCGCGAA GTTGCTGGAA 
AGAGTACCAA TTCCTATTAG GGAAAGATTA GATGAACCTG CTGCCAAGAT TAATGCCCTT 
CTCCAATCAT ATATATCAAG ACAACGCCTG GATGGATTTG CACTTGTGGC AGACATGGTA 
TACGTTACGC AATCAGCGGG TAGAATTATG CGTGCAATAT TCGAGATATC ATTGAGACGT 
GGTTGGTCAT CTGTTGCAAC ACTCTCTCTT GATACATGCA AAATGATTGA GAAAAGGCTT 
TGGCCAACTA TGAGCCCCCT TCGACAGTTT CCCAATTGTC CTTCTGAAGT AATACGCCGC 
GTTGAGAAAA AGGAATTTCC TTGGCAGCGA TACTTTGATC TGGACCCTGC TGAATTAGGA 
GAATTAGTAG GGGTGCCAAA AGAAGGGAGG CGTGTGTACA ACATGGTTCA GTCTTTTCCA 
AGGTTGAGCG TCGAAGCACA TGTTCAACCT ATCACAAGAT CGCTTGTGCG GGTCGAACTT 
GTTATAAATT CTCAGTTCAA CTGGGATGAT CATTTAAGCG GCACTTCTGA AGCTTTTTGG 
ATCCTTGTAG AAGACGTGGA CGGGGACCGA TTGCTACATT ATGAACAATT TTTCCTTCTT 
AAAAAATACA AAGATGATGA GCATATTGTA AATTTTACAG TACCTTTGTT AGAACCTCTT 
CCTCCTTGTT ACTTTATAAA GATAGTTTCA GATCGATGGT TACACTCGAT AACTAAGGTA 
CCTCTTTCAT TTCAGCGCCT GATAATGCCA GAAAAATTTC CTGCGCCAAC ACCTCTTCTT 
GATCTTCAGA ACGCCCCGGT CTCTAGTCTT AATAACCCAA GCTTCATTTC CCTCTACCCT 
AATTTTAAAT TTTTTAACAA GATTCAAACA CAGGTGTTTA ACTCTGTTTA TAAAACCAAC 
GATAGTGTTT TTATTGGTGC TCCCAATGGA AGTGGAAAGA CTGTTTGTGC CGAACTTGCT 
TTGTTACATC ATTGGAGTCA AGAAGATTAT GGTACTGCAG TTTACATTGC CCCAATTCAG 
GAAATCGTTG ATCGTCGATA TGAAGAATGG TATGGCAAGT TTTCTGACCT CGGTGATGGG 
AAAGTTTTGG TAAAATTGAC GGGTGAGCGT TCACAAGACT TGAAGCTGAT ACAAGTTGCT 
GACTTGATAT TTTGCACGCC ATCGCAATGG GACTCATTGT CGAAGAGATG GAGAAGTATG 
CGAAGCATTC AGAAAGTTGA TTTTTATATA TGTGATGAAT TACAATTGCT TGGTGGATTT 
TATGGTCCAT TATACGAAAT AGTGATCTCA CGGATACGGT ACATGGCAGT ACAATTAGAA 
AAAAATATCC GAGTGGTTGG CCTTTCAGTA TCTGTTGCTA ATGCTCGAGA TTTGGGTGAG 
TGGCTTGGGA CTAGTCCACA ATGCATATTT AATTTTAGTC CTAAAGACAG GCCTAATCCT 
CTTACTATTC ATTTGCAATC ATTCAGTATT ACTCATTTCC CTTCCCTTAT GTTGGCTATG 
AGCAAGCCTA TATATCGCAG CTTGAAGAAC TTTATTTCTC AGAGAAAGTC TACTATTGTA 
TTTACTCCTG ACAGGAAAGT AGCGAAACAA TTAGCTTTTG ATTTAGTTAC ATTCAGTATG 
GCTGATGAAG ATGAGTATTT GTTTTCGTTA ATGGAGAATG AAGCTTTCAA TAAAGTTGAA 
GATGCTGCCT TGCAGCAATC ATTGAAGCAT GGTATTGCTT ATATTAGTGA AATCACATCG 
TCCAATGATC AGAATATTGT ACAATATCTT TACCGTCATG GACTTATCAA GGTTTTGATA 
GCTTCTCGCG ATGTTATTTA TTCCTTAAAA GCAAAATCAA ACGCTGTTAT TGTGATGGGT 
ACTCAATACT ATGATGGCAA AGAACATCGT TATATTGATT ATCCTATAAG TGAGTTATTA 
CAAATGCTCG GGTTTACTGC TAGCATTGGA TCAAGTGAGC TTAGCCAGGT TATTCTTATG 
ACTGTCACTA CTAAGAAGGA ATATTACAAG AAATTTTTAA ATGAGCCATT ACCCATGGAA 
AGCCATCTTC AAGTTTGGCT GCACGATGCT TTCGTGTCAG AAATATCTAC GCAAACCATT 
GAAAGCAAAC AAGATGCTGT AGATTGGCTT ACATGGTCGT ATATGTACCG GAGACTAGTT 
GCCAATCCTG CCTATTATGG TCTTCAAGAT ATCACTCATG AGTCGGTTTC TGAATTCCTT 
TCGGATCTTG TAGAAACAAC CATGAATGAT TTGAGTGAGG CACGCTTAAT CACTGTAGAC 
GATGAGGATG ATAGTTGTGT CGCATTAAAT TTAGCAATGA TAGCTTCTCA TTACGGAATT 
ACATACATTA CTATGCAAAC GTTTGCTTTG AGCTTGTCAG AGCGCACGAA GATGAAGGGA 
TTACTAGAAA TTGTTACATC AGCAGCAGAA TATGAGCAAC TCCCAATTCG AAAGTATGAA 
GATATTGTGC TGGAGCGAAT TCATTCCCGC CTGCCTGTTC GCTTAAGTAA TCCTAACTAT 
GAAGATCCTC ATACGAAATC ATTTATTCTT CTTGCAGCTC ATTTTTCTAG ATTTGAATTG 
CCTCCTGGTT TAGTTATAGA TCAGAAATTC ATTTTGACTA GGGTACATAA TCTTCTGGGC 
GCTTGTGTTG ATACATTGTC TTCCGAAGGC CATTTAATAG CTTGTATTAG ACCAATGGAG 
ATGTCGCAAA TGGTTACGCA AGCTTTATGG GATCGTGATA GTCCTTTAAA ACAGATACCC 
TATTTCGACG ACGCTTTAAT CGAACGATGC AATAAAGAGG GTGTTCATGA TGTCTTTGAT 
ATTATCGATT TGGATGACGA AAAACGTACT GAATTGTTAC ATATGGATAA TGCACATCTT 
GCCAAATGCG CAGAGTTTAT CAATAAATAT CCGGATATTG ACATTGATTT TGAAATAGAA 
GACTCTGAAG ATGTTCACGC TAATTCACCC TCTGTGCTTA TTGTTCAACT AACTCGAGAA 
CTGGAAGAGG ATGAAGAAGT TGATACGACT GTCATTGCTC CTTACTTTCC AGCTCAAAAA 
ACTGAACATT GGTGGCTTGT AATTTCGGAT GACAAAACCC TCTTGGCAAT TAAGAAGATT 
ACCTTAGGTA GGTCCTTGAC TACCAAGATG GAATTTGTGC CCCCGGCAAT GGGAACTTTA 
AAATATAAAC TGAGCTGCTT TAGCGATTCT TATATGGGTG TGGATTATGA AAAAGAATTT 
GAATGCAACG TTTTGGAACC TTTGGACACA GAAATGGAAG ACGGGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593346.1
CDS	42..6572
Translation	MSSAHPKGDSKEPPKHGNSKEKPNYGQSQYSYSAMSNLVTQADRRFVSRRDAEPTGEPESLVNRVSIADMGSRARIEKPSTLPLELTQEVQEVRLPRKDAESLEIGIRQPEREKRSSAILKYFDSFEILKYNPLTDETREVYDYILSFIQQYLGDQSPEILRSAADLIIELLKDSSLDEQGRKKQIEEVLSTELPQDRFSQLVNLGNRLTDYTVEQEEELNEEGVNESGVPVLFNEADEEEEAVEAMEEDEVAEDEDVVLETSISQEEEKKNIENPDTEVTFISADTKKVTEIPTVHPREIDAFWLQREIAKYFADAVVCQEKTNQAFEALSADYDLGELENELMSIFDYEHFYLVQLLTKNRWTIVSCTMLKRAATDEERLGVEEQIRAAGRSWILEALRPGAITIPDDGLNELNNNVVEKAEPAPVSEIPLSKTLTSHKIVPKHQVDLENYVFTEGSRLMSNKAVKLPEGSFRRTGKGYEEIHVPAPNKAVLGADERLVKIKELPEWSHQAFLNTQSLNRIQSHLYPIAFGTDENILLCAPTGAGKTNVAMLCILNELQKHLREDLSFNLQNFKIVYIAPLKALVQEMVNNFSKRLTPYNIRVAELTGDSQLTKQQISETQIIVTTPEKWDIITRKANDLSYVNLVRLVIIDEVHLLHDERGPVLESIVARIFRHQEETLEQVRLVGLSATLPNYTDVASFLHVDPKKGLFYFDSTYRPCPLKQEFIGITEKTPFKRMQTTNEACYEKVMQHAGKNQVLIFVHSRKETAKTARFIRDKALEEETIGHLLRSDAASREILRAEADSTSDENLKDLLPYGFAIHHAGMRREDRQTSEDLFADGTIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGIWTELSPQDVLQMLGRAGRPQFDTYGEGIIITAHSELQYYLSLMNQQLPIESQFMRRLADCLNAEVSLGTVRSIEDGVDWLGYTYLYVRMLRSPALYSVGPEYDDDKYLVQKRADLLHSAAILLEKCKLLVYNRQSGTLTATELGKVAASYYVTHNSMAIYNRLLMQTTSFIELFRVFSFSDEFKHIPVREEEKVELAKLLERVPIPIRERLDEPAAKINALLQSYISRQRLDGFALVADMVYVTQSAGRIMRAIFEISLRRGWSSVATLSLDTCKMIEKRLWPTMSPLRQFPNCPSEVIRRVEKKEFPWQRYFDLDPAELGELVGVPKEGRRVYNMVQSFPRLSVEAHVQPITRSLVRVELVINSQFNWDDHLSGTSEAFWILVEDVDGDRLLHYEQFFLLKKYKDDEHIVNFTVPLLEPLPPCYFIKIVSDRWLHSITKVPLSFQRLIMPEKFPAPTPLLDLQNAPVSSLNNPSFISLYPNFKFFNKIQTQVFNSVYKTNDSVFIGAPNGSGKTVCAELALLHHWSQEDYGTAVYIAPIQEIVDRRYEEWYGKFSDLGDGKVLVKLTGERSQDLKLIQVADLIFCTPSQWDSLSKRWRSMRSIQKVDFYICDELQLLGGFYGPLYEIVISRIRYMAVQLEKNIRVVGLSVSVANARDLGEWLGTSPQCIFNFSPKDRPNPLTIHLQSFSITHFPSLMLAMSKPIYRSLKNFISQRKSTIVFTPDRKVAKQLAFDLVTFSMADEDEYLFSLMENEAFNKVEDAALQQSLKHGIAYISEITSSNDQNIVQYLYRHGLIKVLIASRDVIYSLKAKSNAVIVMGTQYYDGKEHRYIDYPISELLQMLGFTASIGSSELSQVILMTVTTKKEYYKKFLNEPLPMESHLQVWLHDAFVSEISTQTIESKQDAVDWLTWSYMYRRLVANPAYYGLQDITHESVSEFLSDLVETTMNDLSEARLITVDDEDDSCVALNLAMIASHYGITYITMQTFALSLSERTKMKGLLEIVTSAAEYEQLPIRKYEDIVLERIHSRLPVRLSNPNYEDPHTKSFILLAAHFSRFELPPGLVIDQKFILTRVHNLLGACVDTLSSEGHLIACIRPMEMSQMVTQALWDRDSPLKQIPYFDDALIERCNKEGVHDVFDIIDLDDEKRTELLHMDNAHLAKCAEFINKYPDIDIDFEIEDSEDVHANSPSVLIVQLTRELEEDEEVDTTVIAPYFPAQKTEHWWLVISDDKTLLAIKKITLGRSLTTKMEFVPPAMGTLKYKLSCFSDSYMGVDYEKEFECNVLEPLDTEMEDGE

Target ORF information:

RefSeq Version	NM_001018778.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative U5 snRNP complex subunit Brr2 (brr2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018778.2

ORF Insert Sequence:

ATGTCGAGTG CACATCCTAA AGGGGACTCG AAAGAGCCTC CCAAACATGG AAATAGTAAG 
GAGAAGCCAA ACTATGGTCA ATCACAGTAT AGTTATTCTG CAATGTCAAA TTTGGTTACG 
CAGGCTGATC GAAGATTTGT GTCACGACGT GATGCTGAAC CTACAGGCGA ACCGGAATCT 
CTGGTAAATC GAGTTAGTAT TGCAGATATG GGTTCTAGAG CGAGAATTGA AAAACCTAGT 
ACCCTTCCTC TCGAATTGAC GCAGGAAGTT CAGGAGGTCC GGTTACCTCG TAAAGATGCA 
GAATCCTTAG AAATTGGTAT ACGGCAACCT GAACGCGAAA AGCGCTCTTC AGCGATACTT 
AAATATTTTG ATTCTTTTGA AATTTTGAAA TATAATCCTC TAACCGACGA GACCCGAGAA 
GTTTATGATT ATATCTTGAG TTTCATTCAA CAATACCTTG GCGATCAGTC TCCTGAAATC 
CTCCGTTCTG CTGCTGATTT GATTATTGAA CTTCTTAAGG ATAGTTCTTT AGATGAACAA 
GGTCGTAAAA AGCAAATAGA GGAGGTTTTG AGTACTGAAC TCCCACAGGA TCGGTTTTCA 
CAGCTCGTTA ACTTGGGAAA TCGCTTAACA GATTATACAG TTGAGCAAGA AGAAGAGTTA 
AATGAAGAAG GCGTTAATGA ATCTGGCGTA CCAGTATTGT TCAATGAAGC CGATGAAGAG 
GAAGAAGCTG TTGAGGCAAT GGAAGAGGAT GAAGTGGCAG AAGATGAAGA CGTCGTCTTA 
GAAACTTCCA TATCTCAAGA AGAAGAGAAA AAGAATATTG AAAATCCGGA TACAGAGGTT 
ACATTTATTT CTGCTGATAC CAAAAAAGTT ACCGAAATAC CCACTGTTCA TCCTCGTGAA 
ATCGATGCAT TTTGGCTGCA ACGAGAAATA GCAAAATATT TTGCTGACGC CGTCGTTTGT 
CAGGAAAAAA CAAATCAAGC GTTTGAGGCA CTTTCGGCTG ATTACGATCT CGGTGAATTG 
GAAAATGAAT TGATGAGTAT TTTTGATTAT GAACATTTCT ATCTTGTTCA ACTTTTGACT 
AAGAATCGTT GGACAATAGT TTCGTGTACA ATGTTAAAAC GCGCCGCTAC TGATGAAGAA 
CGCTTAGGTG TGGAAGAGCA AATTCGTGCT GCTGGTCGGT CTTGGATTCT CGAAGCTCTA 
CGCCCTGGTG CTATCACTAT ACCTGATGAC GGTTTAAATG AATTAAATAA TAATGTTGTT 
GAGAAAGCTG AACCTGCGCC CGTTTCTGAA ATTCCACTTT CGAAAACTTT GACTTCCCAT 
AAAATTGTAC CTAAGCACCA AGTTGATCTA GAAAATTATG TGTTTACTGA GGGATCACGT 
CTCATGTCTA ATAAAGCTGT CAAGCTACCT GAAGGCTCGT TTCGTAGGAC TGGTAAAGGG 
TATGAAGAAA TACATGTTCC TGCCCCCAAC AAAGCCGTAC TTGGCGCTGA CGAGCGTCTT 
GTAAAAATCA AGGAACTGCC TGAATGGTCT CATCAAGCTT TTCTTAATAC TCAATCTTTA 
AACCGAATCC AAAGCCATCT TTATCCTATC GCTTTTGGTA CTGACGAAAA CATCCTTTTA 
TGCGCTCCTA CTGGTGCTGG AAAAACAAAT GTAGCAATGC TATGTATCCT TAACGAATTA 
CAAAAACATT TACGTGAAGA TCTTAGCTTT AACTTACAAA ATTTTAAAAT TGTTTACATT 
GCTCCCTTGA AGGCGTTGGT ACAAGAGATG GTTAATAATT TCTCAAAGAG GCTTACTCCA 
TACAATATTC GAGTCGCAGA GTTAACTGGT GATAGTCAAT TGACTAAACA ACAAATTAGT 
GAAACTCAAA TAATTGTAAC CACACCCGAG AAGTGGGATA TTATAACTCG AAAAGCTAAT 
GATTTATCTT ATGTCAATCT AGTCCGTTTA GTTATTATTG ATGAGGTTCA TTTGCTTCAT 
GATGAACGAG GTCCTGTATT AGAATCTATA GTTGCTCGTA TATTTCGCCA TCAGGAGGAA 
ACTCTTGAAC AAGTTCGATT AGTAGGTTTG TCTGCTACAC TTCCTAATTA TACCGATGTT 
GCCAGTTTTT TACATGTCGA CCCTAAAAAA GGGCTTTTTT ACTTCGATTC GACATATCGT 
CCATGCCCTT TAAAGCAAGA ATTTATAGGC ATTACAGAAA AAACACCGTT TAAACGAATG 
CAGACGACTA ATGAAGCTTG TTATGAAAAA GTTATGCAAC ACGCTGGCAA AAACCAGGTC 
CTCATATTTG TACATTCGCG CAAAGAAACC GCTAAAACCG CTCGTTTTAT AAGAGATAAG 
GCACTAGAGG AAGAGACCAT CGGTCATTTG TTACGCTCTG ATGCCGCATC CCGTGAAATT 
CTTCGGGCTG AAGCCGACTC TACCAGTGAT GAAAATTTGA AAGATTTGCT CCCATATGGG 
TTTGCCATAC ATCATGCTGG TATGCGACGA GAGGATCGCC AAACGTCTGA AGACCTTTTT 
GCCGATGGTA CTATACAGGT ACTAGTGAGT ACGGCAACTC TGGCTTGGGG CGTAAACTTA 
CCAGCGCACA CAGTGATCAT CAAAGGGACC CAAGTTTATT CTCCTGAAAA GGGTATCTGG 
ACAGAACTCT CTCCTCAGGA TGTTTTGCAA ATGCTTGGCA GAGCTGGTCG CCCACAATTC 
GACACATATG GTGAAGGAAT TATCATTACC GCCCACTCTG AGTTACAGTA TTATCTATCA 
CTAATGAATC AGCAGCTTCC AATTGAGTCT CAATTTATGC GCCGTCTAGC TGATTGTTTA 
AATGCCGAAG TATCGTTAGG AACTGTTCGT AGTATCGAAG ATGGCGTAGA CTGGCTTGGT 
TATACATACC TCTATGTTCG AATGTTACGA TCCCCTGCCC TATATAGCGT CGGCCCAGAG 
TATGATGATG ACAAATATCT TGTTCAAAAA AGAGCTGACT TACTTCATTC AGCTGCCATT 
TTATTAGAAA AATGCAAGTT ACTAGTGTAT AATCGTCAGA GTGGTACGTT GACCGCTACG 
GAATTGGGAA AAGTTGCTGC AAGTTATTAC GTTACTCATA ACTCGATGGC CATATATAAT 
CGTCTTTTAA TGCAGACAAC ATCCTTTATC GAGTTATTTC GAGTCTTCAG TTTTTCTGAT 
GAATTTAAGC ACATTCCTGT CCGTGAAGAA GAGAAAGTTG AACTCGCGAA GTTGCTGGAA 
AGAGTACCAA TTCCTATTAG GGAAAGATTA GATGAACCTG CTGCCAAGAT TAATGCCCTT 
CTCCAATCAT ATATATCAAG ACAACGCCTG GATGGATTTG CACTTGTGGC AGACATGGTA 
TACGTTACGC AATCAGCGGG TAGAATTATG CGTGCAATAT TCGAGATATC ATTGAGACGT 
GGTTGGTCAT CTGTTGCAAC ACTCTCTCTT GATACATGCA AAATGATTGA GAAAAGGCTT 
TGGCCAACTA TGAGCCCCCT TCGACAGTTT CCCAATTGTC CTTCTGAAGT AATACGCCGC 
GTTGAGAAAA AGGAATTTCC TTGGCAGCGA TACTTTGATC TGGACCCTGC TGAATTAGGA 
GAATTAGTAG GGGTGCCAAA AGAAGGGAGG CGTGTGTACA ACATGGTTCA GTCTTTTCCA 
AGGTTGAGCG TCGAAGCACA TGTTCAACCT ATCACAAGAT CGCTTGTGCG GGTCGAACTT 
GTTATAAATT CTCAGTTCAA CTGGGATGAT CATTTAAGCG GCACTTCTGA AGCTTTTTGG 
ATCCTTGTAG AAGACGTGGA CGGGGACCGA TTGCTACATT ATGAACAATT TTTCCTTCTT 
AAAAAATACA AAGATGATGA GCATATTGTA AATTTTACAG TACCTTTGTT AGAACCTCTT 
CCTCCTTGTT ACTTTATAAA GATAGTTTCA GATCGATGGT TACACTCGAT AACTAAGGTA 
CCTCTTTCAT TTCAGCGCCT GATAATGCCA GAAAAATTTC CTGCGCCAAC ACCTCTTCTT 
GATCTTCAGA ACGCCCCGGT CTCTAGTCTT AATAACCCAA GCTTCATTTC CCTCTACCCT 
AATTTTAAAT TTTTTAACAA GATTCAAACA CAGGTGTTTA ACTCTGTTTA TAAAACCAAC 
GATAGTGTTT TTATTGGTGC TCCCAATGGA AGTGGAAAGA CTGTTTGTGC CGAACTTGCT 
TTGTTACATC ATTGGAGTCA AGAAGATTAT GGTACTGCAG TTTACATTGC CCCAATTCAG 
GAAATCGTTG ATCGTCGATA TGAAGAATGG TATGGCAAGT TTTCTGACCT CGGTGATGGG 
AAAGTTTTGG TAAAATTGAC GGGTGAGCGT TCACAAGACT TGAAGCTGAT ACAAGTTGCT 
GACTTGATAT TTTGCACGCC ATCGCAATGG GACTCATTGT CGAAGAGATG GAGAAGTATG 
CGAAGCATTC AGAAAGTTGA TTTTTATATA TGTGATGAAT TACAATTGCT TGGTGGATTT 
TATGGTCCAT TATACGAAAT AGTGATCTCA CGGATACGGT ACATGGCAGT ACAATTAGAA 
AAAAATATCC GAGTGGTTGG CCTTTCAGTA TCTGTTGCTA ATGCTCGAGA TTTGGGTGAG 
TGGCTTGGGA CTAGTCCACA ATGCATATTT AATTTTAGTC CTAAAGACAG GCCTAATCCT 
CTTACTATTC ATTTGCAATC ATTCAGTATT ACTCATTTCC CTTCCCTTAT GTTGGCTATG 
AGCAAGCCTA TATATCGCAG CTTGAAGAAC TTTATTTCTC AGAGAAAGTC TACTATTGTA 
TTTACTCCTG ACAGGAAAGT AGCGAAACAA TTAGCTTTTG ATTTAGTTAC ATTCAGTATG 
GCTGATGAAG ATGAGTATTT GTTTTCGTTA ATGGAGAATG AAGCTTTCAA TAAAGTTGAA 
GATGCTGCCT TGCAGCAATC ATTGAAGCAT GGTATTGCTT ATATTAGTGA AATCACATCG 
TCCAATGATC AGAATATTGT ACAATATCTT TACCGTCATG GACTTATCAA GGTTTTGATA 
GCTTCTCGCG ATGTTATTTA TTCCTTAAAA GCAAAATCAA ACGCTGTTAT TGTGATGGGT 
ACTCAATACT ATGATGGCAA AGAACATCGT TATATTGATT ATCCTATAAG TGAGTTATTA 
CAAATGCTCG GGTTTACTGC TAGCATTGGA TCAAGTGAGC TTAGCCAGGT TATTCTTATG 
ACTGTCACTA CTAAGAAGGA ATATTACAAG AAATTTTTAA ATGAGCCATT ACCCATGGAA 
AGCCATCTTC AAGTTTGGCT GCACGATGCT TTCGTGTCAG AAATATCTAC GCAAACCATT 
GAAAGCAAAC AAGATGCTGT AGATTGGCTT ACATGGTCGT ATATGTACCG GAGACTAGTT 
GCCAATCCTG CCTATTATGG TCTTCAAGAT ATCACTCATG AGTCGGTTTC TGAATTCCTT 
TCGGATCTTG TAGAAACAAC CATGAATGAT TTGAGTGAGG CACGCTTAAT CACTGTAGAC 
GATGAGGATG ATAGTTGTGT CGCATTAAAT TTAGCAATGA TAGCTTCTCA TTACGGAATT 
ACATACATTA CTATGCAAAC GTTTGCTTTG AGCTTGTCAG AGCGCACGAA GATGAAGGGA 
TTACTAGAAA TTGTTACATC AGCAGCAGAA TATGAGCAAC TCCCAATTCG AAAGTATGAA 
GATATTGTGC TGGAGCGAAT TCATTCCCGC CTGCCTGTTC GCTTAAGTAA TCCTAACTAT 
GAAGATCCTC ATACGAAATC ATTTATTCTT CTTGCAGCTC ATTTTTCTAG ATTTGAATTG 
CCTCCTGGTT TAGTTATAGA TCAGAAATTC ATTTTGACTA GGGTACATAA TCTTCTGGGC 
GCTTGTGTTG ATACATTGTC TTCCGAAGGC CATTTAATAG CTTGTATTAG ACCAATGGAG 
ATGTCGCAAA TGGTTACGCA AGCTTTATGG GATCGTGATA GTCCTTTAAA ACAGATACCC 
TATTTCGACG ACGCTTTAAT CGAACGATGC AATAAAGAGG GTGTTCATGA TGTCTTTGAT 
ATTATCGATT TGGATGACGA AAAACGTACT GAATTGTTAC ATATGGATAA TGCACATCTT 
GCCAAATGCG CAGAGTTTAT CAATAAATAT CCGGATATTG ACATTGATTT TGAAATAGAA 
GACTCTGAAG ATGTTCACGC TAATTCACCC TCTGTGCTTA TTGTTCAACT AACTCGAGAA 
CTGGAAGAGG ATGAAGAAGT TGATACGACT GTCATTGCTC CTTACTTTCC AGCTCAAAAA 
ACTGAACATT GGTGGCTTGT AATTTCGGAT GACAAAACCC TCTTGGCAAT TAAGAAGATT 
ACCTTAGGTA GGTCCTTGAC TACCAAGATG GAATTTGTGC CCCCGGCAAT GGGAACTTTA 
AAATATAAAC TGAGCTGCTT TAGCGATTCT TATATGGGTG TGGATTATGA AAAAGAATTT 
GAATGCAACG TTTTGGAACC TTTGGACACA GAAATGGAAG ACGGGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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brr2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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