TIL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIL1
Entrez Gene ID	837346
Full Name	DNA polymerase epsilon catalytic subunit
Synonyms	ABA OVERLY SENSITIVE 4,ABO4,EARLY IN SHORT DAYS 7,EMB142,EMB2284,EMB529,EMBRYO DEFECTIVE 142,EMBRYO DEFECTIVE 2284,EMBRYO DEFECTIVE 529,ESD7,POL2A,T23G18.21,T23G18_21,TILTED 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001331765.1	NP_001322767.1	DNA polymerase epsilon catalytic subunit
NM_100699.6	NP_172303.5	DNA polymerase epsilon catalytic subunit

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that the role of Pol epsilon in replicative stress sensing is conserved in plants.
Title: Function of the Plant DNA Polymerase Epsilon in Replicative Stress Sensing, a Genetic Analysis.

We also demonstrate that a domain of the C-terminal region of ESD7 mediates the binding to the different PRC2 components and this interaction is necessary for the proper recruitment of PRC2 to FT and SOC1 chromatin.
Title: Arabidopsis DNA polymerase ? recruits components of Polycomb repressor complex to mediate epigenetic gene silencing.

Data indicate that mutations in DNA polymerase epsilon catalytic subunit A (POL2A) cause reduced fertility and meiotic defects, including abnormal chromosome association, improper chromosome segregation, and fragmentation.
Title: Formation of interference-sensitive meiotic cross-overs requires sufficient DNA leading-strand elongation.

The ESD7 gene encodes the catalytic subunit of DNA polymerase epsilon. The esd7-1 mutant is early flowering and displays pleiotropic defects in both vegetative and reproductive development. [ESD7]
Title: EARLY IN SHORT DAYS 7 (ESD7) encodes the catalytic subunit of DNA polymerase epsilon and is required for flowering repression through a mechanism involving epigenetic gene silencing.

POL2a is involved in maintaining epigenetic states, homologous recombination, and ABA signaling in Arabidopsis.
Title: Epigenetic regulation, somatic homologous recombination, and abscisic acid signaling are influenced by DNA polymerase epsilon mutation in Arabidopsis.

The following TIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb33289	NM_001331765.1 Latest version!	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAb30349	NM_100699.6 Latest version!	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIL1 ( NM_001331765.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322767.1 Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL1), partial mRNA.

Price & Availability↑

CloneID	OAb33289
Clone ID Related Accession (Same CDS sequence)	NM_001331765.1
Accession Version	NM_001331765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). COMPLETENESS: incomplete on the 3' end.

ATGAGCGGAG ATAATCGAAG ACGGGATCGA AAGGATACGC GGTGGTCGAA GAAGCCTAAG 
GTAGTGAACA CTGCGGAAGA TGAACTCGAA TCTAAACTCG GATTTGGGCT TTTCTCTGAA 
GGTGAAACCC GACTTGGGTG GTTACTCACA TTCTCATCGT CATCTTGGGA AGATCGCGAT 
ACTGGGAAAG TCTATAGTTG TGTGGACCTT TACTTTGTTA CTCAGGACGG CTTTTCTTTC 
AAGACAAAGT ACAAGTTCCG TCCTTACTTT TATGCAGCCA CGAAAGACAA AATGGAATTG 
GAACTCGAAG CCTACTTGAG ACGCCGATAT GAAAGACAAG TTGCTGATAT TGAGATTGTT 
GAAAAAGAAG ATCTTGATCT TAAAAATCAT CTATCTGGCC TGCAAAAAAA ATACCTAAAA 
ATATCATTTG ATACTGTGCA ACAATTGATG GAAGTCAAGC GGGATTTGTT ACATATTGTC 
GAACGAAACC AGGCAAAATT TGATGCACTT GAAGCATACG AATCCATATT GGCTGGAAAA 
CGAGAACAAC GTCCTCAAGA TTGCTTAGAC TCTATCGTGG ACCTCCGCGA GTACGATGTA 
CCTTATCATG TTCGATTTGC AATTGATAAT GATGTGCGGA GTGGGCAATG GTACAATGTC 
AGTATTTCCA GCACCGATGT CATATTGGAG AAGAGGACAG ATCTTCTTCA ACGCGCAGAA 
GTCCGTGTTT GTGCCTTCGA TATAGAGACA ACAAAGCTTC CGTTGAAGTT TCCTGATGCT 
GAATATGATC AAATTATGAT GATATCCTAC ATGGTCGATG GGCAAGGTTT TCTAATTATT 
AACAGAGAGT GTGTTGGCGA AGATGTAGAA GATCTGGAAT ATACACCAAA ACCAGAATTT 
GAAGGGTATT TCAAAGTTAC TAATGTTAAA AATGAGGTTG AACTCCTCCA AAGATGGTTT 
TACCATATGC AAGAGTTGAA ACCCGGTATT TATGTTACAT ACAACGGTGA CTTTTTTGAC 
TGGCCATTTA TAGAACGGAG AGCATCTCAT CATGGGATAA AAATGAATGA GGAATTGGGA 
TTCCGTTGTG ATCAGAATCA AGGTGAATGT CGAGCTAAAT TCGCTTGTCA TTTGGATTGT 
TTTGCTTGGG TTAAACGTGA TAGCTATCTT CCTCAGGGAA GCCATGGTCT GAAGGCTGTT 
ACAAAGGCAA AACTAGGTTA TGATCCACTG GAAGTGAACC CAGAGGACAT GGTTCGGTTT 
GCGATGGAAA AGCCCCAGAC AATGGCCTCC TACTCTGTCT CTGATGCTGT TGCAACTTAT 
TACCTATACA TGACATATGT CAATCCATTC ATTTTTTCTC TCGCAACTAT CATCCCAATG 
GTCCCTGATG AGGTTTTACG TAAAGGGAGT GGCACCCTTT GTGAAATGCT TCTGATGGTT 
GAGGCTTACA AGGCAAATGT TGTATGTCCC AACAAAAATC AAGCTGACCC TGAGAAATTC 
TACCAAAATC AACTTCTTGA AAGTGAGACA TATATTGGTG GTCATGTAGA GTGCCTTGAA 
AGTGGTGTTT TCAGATCTGA CATTCCAACC AGTTTTAAAC TCGATTCATC GGCATACCAG 
CAACTGATCG ATAACCTTGG TCGTGATCTG GAATATGCTA TCACTGTAGA AGGAAAAATG 
AGAATGGATT CAATTTCCAA TTATGATGAA GTGAAGGACG AAATCAAGGA GAAGCTTGAG 
AAGTTACGAG ATGACCCTAT ACGGGAAGAG GGGCCTCTTA TTTATCACCT TGATGTCGCA 
GCCATGTATC CAAATATCAT CTTAACAAAC AGGCTTCAGC CACCATCAAT AGTTACGGAT 
GAGATTTGCA CAGCATGTGA TTTCAACCGC CCGGGAAAGA CCTGTCTTAG AAAACTTGAA 
TGGGTTTGGC GTGGTGTGAC ATTCATGGGA AAGAAAAGTG ATTATTACCA TTTGAAGAAG 
CAGATTGAGT CTGAATTTGT TGATGCTGGT GCAAATATAA TGTCTTCAAA ATCGTTCCTT 
GATCTGCCAA AGGTGGATCA ACAATCTAAG CTAAAAGAAC GACTAAAAAA ATATTGTCAA 
AAGGCTTATA AACGAGTACT TGACAAGCCA ATTACTGAAG TTCGTGAAGC TGGGATATGC 
ATGAGAGAAA ACCCTTTCTA TGTGGATACT GTTCGGAGCT TTCGAGATAG GAGGTATGAA 
TACAAAACAC TTAACAAGGT CTGGAAGGGA AAATTGTCAG AGGCCAAGGC AAGTGGTAAT 
TCAATCAAGA TCCAGGAAGC ACAGGACATG GTAGTGGTTT ACGATTCCTT GCAGCTAGCT 
CATAAGTGTA TACTTAATTC CTTCTATGGA TATGTCATGC GAAAGGGTGC AAGATGGTAT 
TCCATGGAAA TGGCTGGTGT AGTTACCTAT ACTGGAGCCA AAATCATACA GAATGCCCGT 
TTGCTTATTG AGCGAATTGG GAAACCACTT GAGTTGGATA CAGATGGTAT ATGGTGTTGT 
CTCCCTGGAT CTTTTCCTGA GAACTTTACT TTTAAAACAA TAGACATGAA GAAACTCACC 
ATCTCGTATC CTTGTGTTAT GCTTAATGTT GATGTGGCAA AGAATAATAC AAATGATCAA 
TATCAGACTC TTGTAGATCC TGTACGCAAG ACATATAAAT CACATAGTGA ATGCTCAATT 
GAATTTGAAG TAGATGGACC GTACAAGGCA ATGATTATTC CTGCATCGAA AGAAGAAGGA 
ATCTTAATCA AGAAGCGTTA TGCTGTTTTC AATCATGATG GAACCTTAGC AGAACTCAAG 
GGGTTTGAGA TCAAGCGTAG AGGGGAGCTG AAACTCATTA AAGTTTTCCA GGCCGAGCTT 
TTTGACAAAT TTCTCCATGG ATCAACTCTT GAAGAGTGTT ATTCTGCTGT TGCAGCGGTT 
GCTGATCGAT GGCTTGACCT ACTCGATAAT CAAGGAAAAG ATATTGCCGA TAGTGAACTG 
CTAGATTATA TATCAGAATC AAGCACAATG AGCAAATCGT TAGCAGATTA TGGTGAGCAA 
AAGTCATGTG CAGTGACCAC AGCAAAACGC CTGGCTGAAT TTCTTGGTGT TACAATGGTC 
AAAGATAAAG GACTACGTTG CCAGTACATT GTTGCTTGTG AACCAAAGGG TACACCGGTA 
AGCGAACGTG CTGTTCCTGT TGCCATATTT ACAACAAATC CGGAAGTTAT GAAGTTTCAT 
TTGCGAAAAT GGTGCAAGAC ATCATCAGAT GTCGGAATTC GTCTGATTAT TGACTGGTCG 
TATTACAAGC AGCGCCTTAG TTCAGCTATC CAAAAAGTTA TTACCATTCC TGCTGCAATG 
CAGAAGGTGG CAAATCCCGT GCCTAGGGTG CTTCATCCTG ACTGGCTCCA TAAAAAGGTC 
CGTGAGAAAG ACGACAAATT TCGCCAGAGA AAACTAGTTG ATATGTTCAG CTCAGCAAAT 
AAAGATGTTG TGTTGGACAC AGATCTTCCG GTGACTAAAG ATAATGTGGA AGATATTGAG 
GATTTTTGCA AAGAAAATAG GCCTTCCGTC AAAGGGCCAA AGCCAATTGC GCGTTCCTAT 
GAAGTCAATA AAAAACAGTC TGAATGTGAG CAGCAAGAGA GCTGGGATAC AGAGTTCCAT 
GATATCTCAT TTCAGAACAT TGACAAGAGC GTAAACTACC AAGGATGGCT TGAACTGAAA 
AAGAGAAAAT GGAAAGTAAC TCTAGAGAAG AAGAAAAAAC GAAGGTTGGG TGATCTAAGA 
TCTTCAAACC AGGTTGATAC TCACGAGATA AATCAAAAAG TCGGTCAGGG AAGAGGAGGT 
GTGGGTTCAT ACTTTAGGAG GCCTGAAGAA GCTTTGACTA GTTCACATTG GCAGATAATA 
CAGCTGGTCC CAAGTCCACA GAGCGGACAA TTTTTTGCTT GGGTGGTTGT GGAAGGGTTG 
ATGCTTAAGA TCCCATTGTC GATCCCAAGG GTGTTTTACA TTAATTCCAA AGTTCCTATA 
GATGAATATT TCCAAGGAAA GTGTGTCAAC AAGATTCTTC CTCATGGAAG GCCTTGTTAT 
AGTTTGACTG AGGTCAAGAT TCAAGAAGAT CAATTTAAAA AAGAAAGTAA AAAGCGTGCA 
GCTCTTCTTG CAGACCCTGG AGTTGAGGGC ATATATGAAA CTAAGGTGCC TCTGGAGTTT 
AGTGCTATAT GTCAGATTGG ATGTGTATGC AAAATTGATA ACAAAGCCAA GCACCGTAAC 
ACCCAAGATG GATGGGAAGT TGGTGAACTT CACATGAAAA CTACAACTGA GTGTCACTAC 
TTGAAGCGTT CAATTCCACT TGTTTACTTG TATAACAGCA CCTCAACAGG GCGTGCTATA 
TATGTTTTAT ATTGTCATGT ATCGAAGCTT ATGTCTGCCG TTGTAGTTGA TCCTTTTAAC 
GGCAATGAAT TATTACCATC TGCCTTAGAG AGACAGTTTA GAGATAGTTG CCTAGAATTA 
TCACTTGATT CATTGTCTTG GGATGGTATC CGTTTCCAGG TGCACTACGT TGACCATCCC 
GAAGCTGCCA AGAAGATAAT ACAAAGAGCA ATAAGTGAAT ACAGAGAAGA AAATTGTGGA 
CCAACTGTTG CTGTCATTGA ATGCCCTGAC TTTACCTTCA TGAAGGAAGG TATTAAGGCC 
CTAGATGATT TCCCATGTGT GAGGATTCCC TTCAACGACG ATGACAATAG TTATCAGCCT 
GTCTCCTGGC AACGTCCAGC TGCAAAAATA GCAATGTTCC GTTGTGCTGC AGCATTTCAG 
TGGTTGGATC GGAGGATTAC CCAATCAAGA TACGCCCATG TGCCTCTGGG GAATTTTGGA 
CTTGATTGGC TAACATTCAC AATAGATATC TTCCTATCCA GGGCACTCCG TGACCAGCAA 
CAGGTCCTTT GGGTGTCAGA TAATGGTGTT CCAGACCTTG GAGGTATAAA CAATGAAGAG 
GCATTCTTTG CCGATGAGGT GCAACAGACA AGTTTAGTAT TCCCTGGAGC GTATAGAAAA 
GTGTCTGTGG AGTTAAAGAT CCATAACTTG GCTGTTAATG CCCTTCTGAA AAGCAACCTG 
GTGAATGAAA TGGAAGGAGG CGGTTTTATG GGATTTGAAC AGGATGTGAA TCCTAGAGGA 
ATTAATTCAA ACGATAACAC CAGTTTTGAT GAGACCACTG GATGTGCTCA AGCATTTCGT 
GTCCTGAAAC AGTTGATTCA TAGTTGCTTG ACTGATGTGC GTAAATCTAA AAATATATAT 
GCTGATTCCA TTTTGCAACG TTTGTCTTGG TGGCTCTGCA GCCCTTCCTC GAAGCTCCAT 
GATCCAGCTC TTCATCTCAT GCTTCACAAG GTCATGCAAA AGGTGTTTGC ACTGCTATTG 
ACTGATTTGC GGAGATTAGG TGCTATAATA ATATATGCAG ACTTCTCAAA GGTCATTATC 
GACACAGTGA AATTTGATCT ATCTGCAGCT AAGGCTTATT GTGAAAGCTT GCTATCAACG 
GTGCGGAATA GTGATATCTT TGAGTGGATA TTGCTCGAGC CGGTTCACTA CTGGCACTCA 
TTACTTTTCA TGGACCAGTA TAACTACGCT GGTATCCGAG CCGATGATGA AATTTCACTT 
GACGAAGTGA CTATTGAACC AAAGTGGAGT GTTGCACGAC ATTTACCTGA GTATATTGAG 
AGGGATTTCA TTATAATCAT TGCCAAGTTT ATATTTGACC CCTGGAAATT TGCGATAGAA 
AATAAAAAGG GCAGTTCAGA GAGTTTAGAG GCACAGATGA TAGAATATCT AAGAGAACAG 
ATTGGATCAA CCTTCATAAA TATGCTTGTC AAAAAGGTTG ATGATATCAT GTCACACATG 
AAGGAGATAA ATGTTTCAGA TGCATCTCGG GTTTCTGGTC AAGCTCCCAA AGGAGATTAC 
TCGTTGGAAT TTATACAGGT TATCTCTGCT GTTCTGGCTC TTGACCAAAA CGTCCAGCAA 
GATGTTCTGG TAATGAGAAA GAGTCTGTTG AAGTACATTA AAGTAAAAGA GTGTGCTGCT 
GAAGCCGAAT TTCTTGATCC TGGGCCATCC TTCATCTTAC CTAATGTAGC TTGCAGGTAT 
TATTTGACCA AAACAAATGC TCGTGATTTA AATAAGCATT CTTGTGATTG GCTACGTCTA 
GTAATAGAGA AATTGTTTTC CGAAATCGAG TTGTCTTTTG GCCACAAACA TAAATCCCTG 
CGAATCCCCT CCTTTAGAGA CATCCTGGTT CACCCTTGTG GTGATCATCA CATCAAATCT 
ACACAGCAAT ATCAAACTTG CAACACTAAA GAAAAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322767.1
CDS	2091..8435
Translation	MSGDNRRRDRKDTRWSKKPKVVNTAEDELESKLGFGLFSEGETRLGWLLTFSSSSWEDRDTGKVYSCVDLYFVTQDGFSFKTKYKFRPYFYAATKDKMELELEAYLRRRYERQVADIEIVEKEDLDLKNHLSGLQKKYLKISFDTVQQLMEVKRDLLHIVERNQAKFDALEAYESILAGKREQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQGFLIINRECVGEDVEDLEYTPKPEFEGYFKVTNVKNEVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVNPFIFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQNQLLESETYIGGHVECLESGVFRSDIPTSFKLDSSAYQQLIDNLGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEICTACDFNRPGKTCLRKLEWVWRGVTFMGKKSDYYHLKKQIESEFVDAGANIMSSKSFLDLPKVDQQSKLKERLKKYCQKAYKRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNSIKIQEAQDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCCLPGSFPENFTFKTIDMKKLTISYPCVMLNVDVAKNNTNDQYQTLVDPVRKTYKSHSECSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVADRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLAEFLGVTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFTTNPEVMKFHLRKWCKTSSDVGIRLIIDWSYYKQRLSSAIQKVITIPAAMQKVANPVPRVLHPDWLHKKVREKDDKFRQRKLVDMFSSANKDVVLDTDLPVTKDNVEDIEDFCKENRPSVKGPKPIARSYEVNKKQSECEQQESWDTEFHDISFQNIDKSVNYQGWLELKKRKWKVTLEKKKKRRLGDLRSSNQVDTHEINQKVGQGRGGVGSYFRRPEEALTSSHWQIIQLVPSPQSGQFFAWVVVEGLMLKIPLSIPRVFYINSKVPIDEYFQGKCVNKILPHGRPCYSLTEVKIQEDQFKKESKKRAALLADPGVEGIYETKVPLEFSAICQIGCVCKIDNKAKHRNTQDGWEVGELHMKTTTECHYLKRSIPLVYLYNSTSTGRAIYVLYCHVSKLMSAVVVDPFNGNELLPSALERQFRDSCLELSLDSLSWDGIRFQVHYVDHPEAAKKIIQRAISEYREENCGPTVAVIECPDFTFMKEGIKALDDFPCVRIPFNDDDNSYQPVSWQRPAAKIAMFRCAAAFQWLDRRITQSRYAHVPLGNFGLDWLTFTIDIFLSRALRDQQQVLWVSDNGVPDLGGINNEEAFFADEVQQTSLVFPGAYRKVSVELKIHNLAVNALLKSNLVNEMEGGGFMGFEQDVNPRGINSNDNTSFDETTGCAQAFRVLKQLIHSCLTDVRKSKNIYADSILQRLSWWLCSPSSKLHDPALHLMLHKVMQKVFALLLTDLRRLGAIIIYADFSKVIIDTVKFDLSAAKAYCESLLSTVRNSDIFEWILLEPVHYWHSLLFMDQYNYAGIRADDEISLDEVTIEPKWSVARHLPEYIERDFIIIIAKFIFDPWKFAIENKKGSSESLEAQMIEYLREQIGSTFINMLVKKVDDIMSHMKEINVSDASRVSGQAPKGDYSLEFIQVISAVLALDQNVQQDVLVMRKSLLKYIKVKECAAEAEFLDPGPSFILPNVACRYYLTKTNARDLNKHSCDWLRLVIEKLFSEIELSFGHKHKSLRIPSFRDILVHPCGDHHIKSTQQYQTCNTKEKKT

Target ORF information:

RefSeq Version	NM_001331765.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331765.1

ORF Insert Sequence:

ATGAGCGGAG ATAATCGAAG ACGGGATCGA AAGGATACGC GGTGGTCGAA GAAGCCTAAG 
GTAGTGAACA CTGCGGAAGA TGAACTCGAA TCTAAACTCG GATTTGGGCT TTTCTCTGAA 
GGTGAAACCC GACTTGGGTG GTTACTCACA TTCTCATCGT CATCTTGGGA AGATCGCGAT 
ACTGGGAAAG TCTATAGTTG TGTGGACCTT TACTTTGTTA CTCAGGACGG CTTTTCTTTC 
AAGACAAAGT ACAAGTTCCG TCCTTACTTT TATGCAGCCA CGAAAGACAA AATGGAATTG 
GAACTCGAAG CCTACTTGAG ACGCCGATAT GAAAGACAAG TTGCTGATAT TGAGATTGTT 
GAAAAAGAAG ATCTTGATCT TAAAAATCAT CTATCTGGCC TGCAAAAAAA ATACCTAAAA 
ATATCATTTG ATACTGTGCA ACAATTGATG GAAGTCAAGC GGGATTTGTT ACATATTGTC 
GAACGAAACC AGGCAAAATT TGATGCACTT GAAGCATACG AATCCATATT GGCTGGAAAA 
CGAGAACAAC GTCCTCAAGA TTGCTTAGAC TCTATCGTGG ACCTCCGCGA GTACGATGTA 
CCTTATCATG TTCGATTTGC AATTGATAAT GATGTGCGGA GTGGGCAATG GTACAATGTC 
AGTATTTCCA GCACCGATGT CATATTGGAG AAGAGGACAG ATCTTCTTCA ACGCGCAGAA 
GTCCGTGTTT GTGCCTTCGA TATAGAGACA ACAAAGCTTC CGTTGAAGTT TCCTGATGCT 
GAATATGATC AAATTATGAT GATATCCTAC ATGGTCGATG GGCAAGGTTT TCTAATTATT 
AACAGAGAGT GTGTTGGCGA AGATGTAGAA GATCTGGAAT ATACACCAAA ACCAGAATTT 
GAAGGGTATT TCAAAGTTAC TAATGTTAAA AATGAGGTTG AACTCCTCCA AAGATGGTTT 
TACCATATGC AAGAGTTGAA ACCCGGTATT TATGTTACAT ACAACGGTGA CTTTTTTGAC 
TGGCCATTTA TAGAACGGAG AGCATCTCAT CATGGGATAA AAATGAATGA GGAATTGGGA 
TTCCGTTGTG ATCAGAATCA AGGTGAATGT CGAGCTAAAT TCGCTTGTCA TTTGGATTGT 
TTTGCTTGGG TTAAACGTGA TAGCTATCTT CCTCAGGGAA GCCATGGTCT GAAGGCTGTT 
ACAAAGGCAA AACTAGGTTA TGATCCACTG GAAGTGAACC CAGAGGACAT GGTTCGGTTT 
GCGATGGAAA AGCCCCAGAC AATGGCCTCC TACTCTGTCT CTGATGCTGT TGCAACTTAT 
TACCTATACA TGACATATGT CAATCCATTC ATTTTTTCTC TCGCAACTAT CATCCCAATG 
GTCCCTGATG AGGTTTTACG TAAAGGGAGT GGCACCCTTT GTGAAATGCT TCTGATGGTT 
GAGGCTTACA AGGCAAATGT TGTATGTCCC AACAAAAATC AAGCTGACCC TGAGAAATTC 
TACCAAAATC AACTTCTTGA AAGTGAGACA TATATTGGTG GTCATGTAGA GTGCCTTGAA 
AGTGGTGTTT TCAGATCTGA CATTCCAACC AGTTTTAAAC TCGATTCATC GGCATACCAG 
CAACTGATCG ATAACCTTGG TCGTGATCTG GAATATGCTA TCACTGTAGA AGGAAAAATG 
AGAATGGATT CAATTTCCAA TTATGATGAA GTGAAGGACG AAATCAAGGA GAAGCTTGAG 
AAGTTACGAG ATGACCCTAT ACGGGAAGAG GGGCCTCTTA TTTATCACCT TGATGTCGCA 
GCCATGTATC CAAATATCAT CTTAACAAAC AGGCTTCAGC CACCATCAAT AGTTACGGAT 
GAGATTTGCA CAGCATGTGA TTTCAACCGC CCGGGAAAGA CCTGTCTTAG AAAACTTGAA 
TGGGTTTGGC GTGGTGTGAC ATTCATGGGA AAGAAAAGTG ATTATTACCA TTTGAAGAAG 
CAGATTGAGT CTGAATTTGT TGATGCTGGT GCAAATATAA TGTCTTCAAA ATCGTTCCTT 
GATCTGCCAA AGGTGGATCA ACAATCTAAG CTAAAAGAAC GACTAAAAAA ATATTGTCAA 
AAGGCTTATA AACGAGTACT TGACAAGCCA ATTACTGAAG TTCGTGAAGC TGGGATATGC 
ATGAGAGAAA ACCCTTTCTA TGTGGATACT GTTCGGAGCT TTCGAGATAG GAGGTATGAA 
TACAAAACAC TTAACAAGGT CTGGAAGGGA AAATTGTCAG AGGCCAAGGC AAGTGGTAAT 
TCAATCAAGA TCCAGGAAGC ACAGGACATG GTAGTGGTTT ACGATTCCTT GCAGCTAGCT 
CATAAGTGTA TACTTAATTC CTTCTATGGA TATGTCATGC GAAAGGGTGC AAGATGGTAT 
TCCATGGAAA TGGCTGGTGT AGTTACCTAT ACTGGAGCCA AAATCATACA GAATGCCCGT 
TTGCTTATTG AGCGAATTGG GAAACCACTT GAGTTGGATA CAGATGGTAT ATGGTGTTGT 
CTCCCTGGAT CTTTTCCTGA GAACTTTACT TTTAAAACAA TAGACATGAA GAAACTCACC 
ATCTCGTATC CTTGTGTTAT GCTTAATGTT GATGTGGCAA AGAATAATAC AAATGATCAA 
TATCAGACTC TTGTAGATCC TGTACGCAAG ACATATAAAT CACATAGTGA ATGCTCAATT 
GAATTTGAAG TAGATGGACC GTACAAGGCA ATGATTATTC CTGCATCGAA AGAAGAAGGA 
ATCTTAATCA AGAAGCGTTA TGCTGTTTTC AATCATGATG GAACCTTAGC AGAACTCAAG 
GGGTTTGAGA TCAAGCGTAG AGGGGAGCTG AAACTCATTA AAGTTTTCCA GGCCGAGCTT 
TTTGACAAAT TTCTCCATGG ATCAACTCTT GAAGAGTGTT ATTCTGCTGT TGCAGCGGTT 
GCTGATCGAT GGCTTGACCT ACTCGATAAT CAAGGAAAAG ATATTGCCGA TAGTGAACTG 
CTAGATTATA TATCAGAATC AAGCACAATG AGCAAATCGT TAGCAGATTA TGGTGAGCAA 
AAGTCATGTG CAGTGACCAC AGCAAAACGC CTGGCTGAAT TTCTTGGTGT TACAATGGTC 
AAAGATAAAG GACTACGTTG CCAGTACATT GTTGCTTGTG AACCAAAGGG TACACCGGTA 
AGCGAACGTG CTGTTCCTGT TGCCATATTT ACAACAAATC CGGAAGTTAT GAAGTTTCAT 
TTGCGAAAAT GGTGCAAGAC ATCATCAGAT GTCGGAATTC GTCTGATTAT TGACTGGTCG 
TATTACAAGC AGCGCCTTAG TTCAGCTATC CAAAAAGTTA TTACCATTCC TGCTGCAATG 
CAGAAGGTGG CAAATCCCGT GCCTAGGGTG CTTCATCCTG ACTGGCTCCA TAAAAAGGTC 
CGTGAGAAAG ACGACAAATT TCGCCAGAGA AAACTAGTTG ATATGTTCAG CTCAGCAAAT 
AAAGATGTTG TGTTGGACAC AGATCTTCCG GTGACTAAAG ATAATGTGGA AGATATTGAG 
GATTTTTGCA AAGAAAATAG GCCTTCCGTC AAAGGGCCAA AGCCAATTGC GCGTTCCTAT 
GAAGTCAATA AAAAACAGTC TGAATGTGAG CAGCAAGAGA GCTGGGATAC AGAGTTCCAT 
GATATCTCAT TTCAGAACAT TGACAAGAGC GTAAACTACC AAGGATGGCT TGAACTGAAA 
AAGAGAAAAT GGAAAGTAAC TCTAGAGAAG AAGAAAAAAC GAAGGTTGGG TGATCTAAGA 
TCTTCAAACC AGGTTGATAC TCACGAGATA AATCAAAAAG TCGGTCAGGG AAGAGGAGGT 
GTGGGTTCAT ACTTTAGGAG GCCTGAAGAA GCTTTGACTA GTTCACATTG GCAGATAATA 
CAGCTGGTCC CAAGTCCACA GAGCGGACAA TTTTTTGCTT GGGTGGTTGT GGAAGGGTTG 
ATGCTTAAGA TCCCATTGTC GATCCCAAGG GTGTTTTACA TTAATTCCAA AGTTCCTATA 
GATGAATATT TCCAAGGAAA GTGTGTCAAC AAGATTCTTC CTCATGGAAG GCCTTGTTAT 
AGTTTGACTG AGGTCAAGAT TCAAGAAGAT CAATTTAAAA AAGAAAGTAA AAAGCGTGCA 
GCTCTTCTTG CAGACCCTGG AGTTGAGGGC ATATATGAAA CTAAGGTGCC TCTGGAGTTT 
AGTGCTATAT GTCAGATTGG ATGTGTATGC AAAATTGATA ACAAAGCCAA GCACCGTAAC 
ACCCAAGATG GATGGGAAGT TGGTGAACTT CACATGAAAA CTACAACTGA GTGTCACTAC 
TTGAAGCGTT CAATTCCACT TGTTTACTTG TATAACAGCA CCTCAACAGG GCGTGCTATA 
TATGTTTTAT ATTGTCATGT ATCGAAGCTT ATGTCTGCCG TTGTAGTTGA TCCTTTTAAC 
GGCAATGAAT TATTACCATC TGCCTTAGAG AGACAGTTTA GAGATAGTTG CCTAGAATTA 
TCACTTGATT CATTGTCTTG GGATGGTATC CGTTTCCAGG TGCACTACGT TGACCATCCC 
GAAGCTGCCA AGAAGATAAT ACAAAGAGCA ATAAGTGAAT ACAGAGAAGA AAATTGTGGA 
CCAACTGTTG CTGTCATTGA ATGCCCTGAC TTTACCTTCA TGAAGGAAGG TATTAAGGCC 
CTAGATGATT TCCCATGTGT GAGGATTCCC TTCAACGACG ATGACAATAG TTATCAGCCT 
GTCTCCTGGC AACGTCCAGC TGCAAAAATA GCAATGTTCC GTTGTGCTGC AGCATTTCAG 
TGGTTGGATC GGAGGATTAC CCAATCAAGA TACGCCCATG TGCCTCTGGG GAATTTTGGA 
CTTGATTGGC TAACATTCAC AATAGATATC TTCCTATCCA GGGCACTCCG TGACCAGCAA 
CAGGTCCTTT GGGTGTCAGA TAATGGTGTT CCAGACCTTG GAGGTATAAA CAATGAAGAG 
GCATTCTTTG CCGATGAGGT GCAACAGACA AGTTTAGTAT TCCCTGGAGC GTATAGAAAA 
GTGTCTGTGG AGTTAAAGAT CCATAACTTG GCTGTTAATG CCCTTCTGAA AAGCAACCTG 
GTGAATGAAA TGGAAGGAGG CGGTTTTATG GGATTTGAAC AGGATGTGAA TCCTAGAGGA 
ATTAATTCAA ACGATAACAC CAGTTTTGAT GAGACCACTG GATGTGCTCA AGCATTTCGT 
GTCCTGAAAC AGTTGATTCA TAGTTGCTTG ACTGATGTGC GTAAATCTAA AAATATATAT 
GCTGATTCCA TTTTGCAACG TTTGTCTTGG TGGCTCTGCA GCCCTTCCTC GAAGCTCCAT 
GATCCAGCTC TTCATCTCAT GCTTCACAAG GTCATGCAAA AGGTGTTTGC ACTGCTATTG 
ACTGATTTGC GGAGATTAGG TGCTATAATA ATATATGCAG ACTTCTCAAA GGTCATTATC 
GACACAGTGA AATTTGATCT ATCTGCAGCT AAGGCTTATT GTGAAAGCTT GCTATCAACG 
GTGCGGAATA GTGATATCTT TGAGTGGATA TTGCTCGAGC CGGTTCACTA CTGGCACTCA 
TTACTTTTCA TGGACCAGTA TAACTACGCT GGTATCCGAG CCGATGATGA AATTTCACTT 
GACGAAGTGA CTATTGAACC AAAGTGGAGT GTTGCACGAC ATTTACCTGA GTATATTGAG 
AGGGATTTCA TTATAATCAT TGCCAAGTTT ATATTTGACC CCTGGAAATT TGCGATAGAA 
AATAAAAAGG GCAGTTCAGA GAGTTTAGAG GCACAGATGA TAGAATATCT AAGAGAACAG 
ATTGGATCAA CCTTCATAAA TATGCTTGTC AAAAAGGTTG ATGATATCAT GTCACACATG 
AAGGAGATAA ATGTTTCAGA TGCATCTCGG GTTTCTGGTC AAGCTCCCAA AGGAGATTAC 
TCGTTGGAAT TTATACAGGT TATCTCTGCT GTTCTGGCTC TTGACCAAAA CGTCCAGCAA 
GATGTTCTGG TAATGAGAAA GAGTCTGTTG AAGTACATTA AAGTAAAAGA GTGTGCTGCT 
GAAGCCGAAT TTCTTGATCC TGGGCCATCC TTCATCTTAC CTAATGTAGC TTGCAGGTAT 
TATTTGACCA AAACAAATGC TCGTGATTTA AATAAGCATT CTTGTGATTG GCTACGTCTA 
GTAATAGAGA AATTGTTTTC CGAAATCGAG TTGTCTTTTG GCCACAAACA TAAATCCCTG 
CGAATCCCCT CCTTTAGAGA CATCCTGGTT CACCCTTGTG GTGATCATCA CATCAAATCT 
ACACAGCAAT ATCAAACTTG CAACACTAAA GAAAAGAAAA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIL1 ( NM_100699.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_172303.5 Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL1), partial mRNA.

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CloneID	OAb30349
Clone ID Related Accession (Same CDS sequence)	NM_100699.6
Accession Version	NM_100699.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100699.5. COMPLETENESS: incomplete on the 3' end.

ATGAGCGGAG ATAATCGAAG ACGGGATCGA AAGGATACGC GGTGGTCGAA GAAGCCTAAG 
GTAGTGAACA CTGCGGAAGA TGAACTCGAA TCTAAACTCG GATTTGGGCT TTTCTCTGAA 
GGTGAAACCC GACTTGGGTG GTTACTCACA TTCTCATCGT CATCTTGGGA AGATCGCGAT 
ACTGGGAAAG TCTATAGTTG TGTGGACCTT TACTTTGTTA CTCAGGACGG CTTTTCTTTC 
AAGACAAAGT ACAAGTTCCG TCCTTACTTT TATGCAGCCA CGAAAGACAA AATGGAATTG 
GAACTCGAAG CCTACTTGAG ACGCCGATAT GAAAGACAAG TTGCTGATAT TGAGATTGTT 
GAAAAAGAAG ATCTTGATCT TAAAAATCAT CTATCTGGCC TGCAAAAAAA ATACCTAAAA 
ATATCATTTG ATACTGTGCA ACAATTGATG GAAGTCAAGC GGGATTTGTT ACATATTGTC 
GAACGAAACC AGGCAAAATT TGATGCACTT GAAGCATACG AATCCATATT GGCTGGAAAA 
CGAGAACAAC GTCCTCAAGA TTGCTTAGAC TCTATCGTGG ACCTCCGCGA GTACGATGTA 
CCTTATCATG TTCGATTTGC AATTGATAAT GATGTGCGGA GTGGGCAATG GTACAATGTC 
AGTATTTCCA GCACCGATGT CATATTGGAG AAGAGGACAG ATCTTCTTCA ACGCGCAGAA 
GTCCGTGTTT GTGCCTTCGA TATAGAGACA ACAAAGCTTC CGTTGAAGTT TCCTGATGCT 
GAATATGATC AAATTATGAT GATATCCTAC ATGGTCGATG GGCAAGGTTT TCTAATTATT 
AACAGAGAGT GTGTTGGCGA AGATGTAGAA GATCTGGAAT ATACACCAAA ACCAGAATTT 
GAAGGGTATT TCAAAGTTAC TAATGTTAAA AATGAGGTTG AACTCCTCCA AAGATGGTTT 
TACCATATGC AAGAGTTGAA ACCCGGTATT TATGTTACAT ACAACGGTGA CTTTTTTGAC 
TGGCCATTTA TAGAACGGAG AGCATCTCAT CATGGGATAA AAATGAATGA GGAATTGGGA 
TTCCGTTGTG ATCAGAATCA AGGTGAATGT CGAGCTAAAT TCGCTTGTCA TTTGGATTGT 
TTTGCTTGGG TTAAACGTGA TAGCTATCTT CCTCAGGGAA GCCATGGTCT GAAGGCTGTT 
ACAAAGGCAA AACTAGGTTA TGATCCACTG GAAGTGAACC CAGAGGACAT GGTTCGGTTT 
GCGATGGAAA AGCCCCAGAC AATGGCCTCC TACTCTGTCT CTGATGCTGT TGCAACTTAT 
TACCTATACA TGACATATGT CAATCCATTC ATTTTTTCTC TCGCAACTAT CATCCCAATG 
GTCCCTGATG AGGTTTTACG TAAAGGGAGT GGCACCCTTT GTGAAATGCT TCTGATGGTT 
GAGGCTTACA AGGCAAATGT TGTATGTCCC AACAAAAATC AAGCTGACCC TGAGAAATTC 
TACCAAAATC AACTTCTTGA AAGTGAGACA TATATTGGTG GTCATGTAGA GTGCCTTGAA 
AGTGGTGTTT TCAGATCTGA CATTCCAACC AGTTTTAAAC TCGATTCATC GGCATACCAG 
CAACTGATCG ATAACCTTGG TCGTGATCTG GAATATGCTA TCACTGTAGA AGGAAAAATG 
AGAATGGATT CAATTTCCAA TTATGATGAA GTGAAGGACG AAATCAAGGA GAAGCTTGAG 
AAGTTACGAG ATGACCCTAT ACGGGAAGAG GGGCCTCTTA TTTATCACCT TGATGTCGCA 
GCCATGTATC CAAATATCAT CTTAACAAAC AGGCTTCAGC CACCATCAAT AGTTACGGAT 
GAGATTTGCA CAGCATGTGA TTTCAACCGC CCGGGAAAGA CCTGTCTTAG AAAACTTGAA 
TGGGTTTGGC GTGGTGTGAC ATTCATGGGA AAGAAAAGTG ATTATTACCA TTTGAAGAAG 
CAGATTGAGT CTGAATTTGT TGATGCTGGT GCAAATATAA TGTCTTCAAA ATCGTTCCTT 
GATCTGCCAA AGGTGGATCA ACAATCTAAG CTAAAAGAAC GACTAAAAAA ATATTGTCAA 
AAGGCTTATA AACGAGTACT TGACAAGCCA ATTACTGAAG TTCGTGAAGC TGGGATATGC 
ATGAGAGAAA ACCCTTTCTA TGTGGATACT GTTCGGAGCT TTCGAGATAG GAGGTATGAA 
TACAAAACAC TTAACAAGGT CTGGAAGGGA AAATTGTCAG AGGCCAAGGC AAGTGGTAAT 
TCAATCAAGA TCCAGGAAGC ACAGGACATG GTAGTGGTTT ACGATTCCTT GCAGCTAGCT 
CATAAGTGTA TACTTAATTC CTTCTATGGA TATGTCATGC GAAAGGGTGC AAGATGGTAT 
TCCATGGAAA TGGCTGGTGT AGTTACCTAT ACTGGAGCCA AAATCATACA GAATGCCCGT 
TTGCTTATTG AGCGAATTGG GAAACCACTT GAGTTGGATA CAGATGGTAT ATGGTGTTGT 
CTCCCTGGAT CTTTTCCTGA GAACTTTACT TTTAAAACAA TAGACATGAA GAAACTCACC 
ATCTCGTATC CTTGTGTTAT GCTTAATGTT GATGTGGCAA AGAATAATAC AAATGATCAA 
TATCAGACTC TTGTAGATCC TGTACGCAAG ACATATAAAT CACATAGTGA ATGCTCAATT 
GAATTTGAAG TAGATGGACC GTACAAGGCA ATGATTATTC CTGCATCGAA AGAAGAAGGA 
ATCTTAATCA AGAAGCGTTA TGCTGTTTTC AATCATGATG GAACCTTAGC AGAACTCAAG 
GGGTTTGAGA TCAAGCGTAG AGGGGAGCTG AAACTCATTA AAGTTTTCCA GGCCGAGCTT 
TTTGACAAAT TTCTCCATGG ATCAACTCTT GAAGAGTGTT ATTCTGCTGT TGCAGCGGTT 
GCTGATCGAT GGCTTGACCT ACTCGATAAT CAAGGAAAAG ATATTGCCGA TAGTGAACTG 
CTAGATTATA TATCAGAATC AAGCACAATG AGCAAATCGT TAGCAGATTA TGGTGAGCAA 
AAGTCATGTG CAGTGACCAC AGCAAAACGC CTGGCTGAAT TTCTTGGTGT TACAATGGTC 
AAAGATAAAG GACTACGTTG CCAGTACATT GTTGCTTGTG AACCAAAGGG TACACCGGTA 
AGCGAACGTG CTGTTCCTGT TGCCATATTT ACAACAAATC CGGAAGTTAT GAAGTTTCAT 
TTGCGAAAAT GGTGCAAGAC ATCATCAGAT GTCGGAATTC GTCTGATTAT TGACTGGTCG 
TATTACAAGC AGCGCCTTAG TTCAGCTATC CAAAAAGTTA TTACCATTCC TGCTGCAATG 
CAGAAGGTGG CAAATCCCGT GCCTAGGGTG CTTCATCCTG ACTGGCTCCA TAAAAAGGTC 
CGTGAGAAAG ACGACAAATT TCGCCAGAGA AAACTAGTTG ATATGTTCAG CTCAGCAAAT 
AAAGATGTTG TGTTGGACAC AGATCTTCCG GTGACTAAAG ATAATGTGGA AGATATTGAG 
GATTTTTGCA AAGAAAATAG GCCTTCCGTC AAAGGGCCAA AGCCAATTGC GCGTTCCTAT 
GAAGTCAATA AAAAACAGTC TGAATGTGAG CAGCAAGAGA GCTGGGATAC AGAGTTCCAT 
GATATCTCAT TTCAGAACAT TGACAAGAGC GTAAACTACC AAGGATGGCT TGAACTGAAA 
AAGAGAAAAT GGAAAGTAAC TCTAGAGAAG AAGAAAAAAC GAAGGTTGGG TGATCTAAGA 
TCTTCAAACC AGGTTGATAC TCACGAGATA AATCAAAAAG TCGGTCAGGG AAGAGGAGGT 
GTGGGTTCAT ACTTTAGGAG GCCTGAAGAA GCTTTGACTA GTTCACATTG GCAGATAATA 
CAGCTGGTCC CAAGTCCACA GAGCGGACAA TTTTTTGCTT GGGTGGTTGT GGAAGGGTTG 
ATGCTTAAGA TCCCATTGTC GATCCCAAGG GTGTTTTACA TTAATTCCAA AGTTCCTATA 
GATGAATATT TCCAAGGAAA GTGTGTCAAC AAGATTCTTC CTCATGGAAG GCCTTGTTAT 
AGTTTGACTG AGGTCAAGAT TCAAGAAGAT CAATTTAAAA AAGAAAGTAA AAAGCGTGCA 
GCTCTTCTTG CAGACCCTGG AGTTGAGGGC ATATATGAAA CTAAGGTGCC TCTGGAGTTT 
AGTGCTATAT GTCAGATTGG ATGTGTATGC AAAATTGATA ACAAAGCCAA GCACCGTAAC 
ACCCAAGATG GATGGGAAGT TGGTGAACTT CACATGAAAA CTACAACTGA GTGTCACTAC 
TTGAAGCGTT CAATTCCACT TGTTTACTTG TATAACAGCA CCTCAACAGG GCGTGCTATA 
TATGTTTTAT ATTGTCATGT ATCGAAGCTT ATGTCTGCCG TTGTAGTTGA TCCTTTTAAC 
GGCAATGAAT TATTACCATC TGCCTTAGAG AGACAGTTTA GAGATAGTTG CCTAGAATTA 
TCACTTGATT CATTGTCTTG GGATGGTATC CGTTTCCAGG TGCACTACGT TGACCATCCC 
GAAGCTGCCA AGAAGATAAT ACAAAGAGCA ATAAGTGAAT ACAGAGAAGA AAATTGTGGA 
CCAACTGTTG CTGTCATTGA ATGCCCTGAC TTTACCTTCA TGAAGGAAGG TATTAAGGCC 
CTAGATGATT TCCCATGTGT GAGGATTCCC TTCAACGACG ATGACAATAG TTATCAGCCT 
GTCTCCTGGC AACGTCCAGC TGCAAAAATA GCAATGTTCC GTTGTGCTGC AGCATTTCAG 
TGGTTGGATC GGAGGATTAC CCAATCAAGA TACGCCCATG TGCCTCTGGG GAATTTTGGA 
CTTGATTGGC TAACATTCAC AATAGATATC TTCCTATCCA GGGCACTCCG TGACCAGCAA 
CAGGTCCTTT GGGTGTCAGA TAATGGTGTT CCAGACCTTG GAGGTATAAA CAATGAAGAG 
GCATTCTTTG CCGATGAGGT GCAACAGACA AGTTTAGTAT TCCCTGGAGC GTATAGAAAA 
GTGTCTGTGG AGTTAAAGAT CCATAACTTG GCTGTTAATG CCCTTCTGAA AAGCAACCTG 
GTGAATGAAA TGGAAGGAGG CGGTTTTATG GGATTTGAAC AGGATGTGAA TCCTAGAGGA 
ATTAATTCAA ACGATAACAC CAGTTTTGAT GAGACCACTG GATGTGCTCA AGCATTTCGT 
GTCCTGAAAC AGTTGATTCA TAGTTGCTTG ACTGATGTGC GTAAATCTAA AAATATATAT 
GCTGATTCCA TTTTGCAACG TTTGTCTTGG TGGCTCTGCA GCCCTTCCTC GAAGCTCCAT 
GATCCAGCTC TTCATCTCAT GCTTCACAAG GTCATGCAAA AGGTGTTTGC ACTGCTATTG 
ACTGATTTGC GGAGATTAGG TGCTATAATA ATATATGCAG ACTTCTCAAA GGTCATTATC 
GACACAGTGA AATTTGATCT ATCTGCAGCT AAGGCTTATT GTGAAAGCTT GCTATCAACG 
GTGCGGAATA GTGATATCTT TGAGTGGATA TTGCTCGAGC CGGTTCACTA CTGGCACTCA 
TTACTTTTCA TGGACCAGTA TAACTACGCT GGTATCCGAG CCGATGATGA AATTTCACTT 
GACGAAGTGA CTATTGAACC AAAGTGGAGT GTTGCACGAC ATTTACCTGA GTATATTGAG 
AGGGATTTCA TTATAATCAT TGCCAAGTTT ATATTTGACC CCTGGAAATT TGCGATAGAA 
AATAAAAAGG GCAGTTCAGA GAGTTTAGAG GCACAGATGA TAGAATATCT AAGAGAACAG 
ATTGGATCAA CCTTCATAAA TATGCTTGTC AAAAAGGTTG ATGATATCAT GTCACACATG 
AAGGAGATAA ATGTTTCAGA TGCATCTCGG GTTTCTGGTC AAGCTCCCAA AGGAGATTAC 
TCGTTGGAAT TTATACAGGT TATCTCTGCT GTTCTGGCTC TTGACCAAAA CGTCCAGCAA 
GATGTTCTGG TAATGAGAAA GAGTCTGTTG AAGTACATTA AAGTAAAAGA GTGTGCTGCT 
GAAGCCGAAT TTCTTGATCC TGGGCCATCC TTCATCTTAC CTAATGTAGC TTGCAGCAAC 
TGTGATGCCT ACAGAGACCT AGATATATGC AGAGACCCAG CACTGTTAAC AGAGAAAGAA 
TGGTCATGTG CGGATACACA ATGTGGGAAG ATATATGACA GAGAGCAGAT GGAGAGTAGC 
CTTCTAGAGA TGGTGAGACA GAGAGAAAGA ATGTACCATA TGCAGGATGT AGTGTGCATT 
AGGTGCAATC AGGTGAAAGC GGCTCATTTG ACAGAGCAGT GTGAGTGTTC TGGTTCCTTT 
AGATGCAAGG AGAGTGGGTC AGAGTTCAGC AAGAGGATGG AGATATTCAT GGATATAGCA 
AAGCGCCAGA AGTTTAGACT GTTAGAAGAA TACATCTCTT GGATCATATA TGGCCCTAGC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_172303.5
CDS	2091..8576
Translation	MSGDNRRRDRKDTRWSKKPKVVNTAEDELESKLGFGLFSEGETRLGWLLTFSSSSWEDRDTGKVYSCVDLYFVTQDGFSFKTKYKFRPYFYAATKDKMELELEAYLRRRYERQVADIEIVEKEDLDLKNHLSGLQKKYLKISFDTVQQLMEVKRDLLHIVERNQAKFDALEAYESILAGKREQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQGFLIINRECVGEDVEDLEYTPKPEFEGYFKVTNVKNEVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVNPFIFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQNQLLESETYIGGHVECLESGVFRSDIPTSFKLDSSAYQQLIDNLGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEICTACDFNRPGKTCLRKLEWVWRGVTFMGKKSDYYHLKKQIESEFVDAGANIMSSKSFLDLPKVDQQSKLKERLKKYCQKAYKRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNSIKIQEAQDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCCLPGSFPENFTFKTIDMKKLTISYPCVMLNVDVAKNNTNDQYQTLVDPVRKTYKSHSECSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVADRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLAEFLGVTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFTTNPEVMKFHLRKWCKTSSDVGIRLIIDWSYYKQRLSSAIQKVITIPAAMQKVANPVPRVLHPDWLHKKVREKDDKFRQRKLVDMFSSANKDVVLDTDLPVTKDNVEDIEDFCKENRPSVKGPKPIARSYEVNKKQSECEQQESWDTEFHDISFQNIDKSVNYQGWLELKKRKWKVTLEKKKKRRLGDLRSSNQVDTHEINQKVGQGRGGVGSYFRRPEEALTSSHWQIIQLVPSPQSGQFFAWVVVEGLMLKIPLSIPRVFYINSKVPIDEYFQGKCVNKILPHGRPCYSLTEVKIQEDQFKKESKKRAALLADPGVEGIYETKVPLEFSAICQIGCVCKIDNKAKHRNTQDGWEVGELHMKTTTECHYLKRSIPLVYLYNSTSTGRAIYVLYCHVSKLMSAVVVDPFNGNELLPSALERQFRDSCLELSLDSLSWDGIRFQVHYVDHPEAAKKIIQRAISEYREENCGPTVAVIECPDFTFMKEGIKALDDFPCVRIPFNDDDNSYQPVSWQRPAAKIAMFRCAAAFQWLDRRITQSRYAHVPLGNFGLDWLTFTIDIFLSRALRDQQQVLWVSDNGVPDLGGINNEEAFFADEVQQTSLVFPGAYRKVSVELKIHNLAVNALLKSNLVNEMEGGGFMGFEQDVNPRGINSNDNTSFDETTGCAQAFRVLKQLIHSCLTDVRKSKNIYADSILQRLSWWLCSPSSKLHDPALHLMLHKVMQKVFALLLTDLRRLGAIIIYADFSKVIIDTVKFDLSAAKAYCESLLSTVRNSDIFEWILLEPVHYWHSLLFMDQYNYAGIRADDEISLDEVTIEPKWSVARHLPEYIERDFIIIIAKFIFDPWKFAIENKKGSSESLEAQMIEYLREQIGSTFINMLVKKVDDIMSHMKEINVSDASRVSGQAPKGDYSLEFIQVISAVLALDQNVQQDVLVMRKSLLKYIKVKECAAEAEFLDPGPSFILPNVACSNCDAYRDLDICRDPALLTEKEWSCADTQCGKIYDREQMESSLLEMVRQRERMYHMQDVVCIRCNQVKAAHLTEQCECSGSFRCKESGSEFSKRMEIFMDIAKRQKFRLLEEYISWIIYGPSY

Target ORF information:

RefSeq Version	NM_100699.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100699.6

ORF Insert Sequence:

ATGAGCGGAG ATAATCGAAG ACGGGATCGA AAGGATACGC GGTGGTCGAA GAAGCCTAAG 
GTAGTGAACA CTGCGGAAGA TGAACTCGAA TCTAAACTCG GATTTGGGCT TTTCTCTGAA 
GGTGAAACCC GACTTGGGTG GTTACTCACA TTCTCATCGT CATCTTGGGA AGATCGCGAT 
ACTGGGAAAG TCTATAGTTG TGTGGACCTT TACTTTGTTA CTCAGGACGG CTTTTCTTTC 
AAGACAAAGT ACAAGTTCCG TCCTTACTTT TATGCAGCCA CGAAAGACAA AATGGAATTG 
GAACTCGAAG CCTACTTGAG ACGCCGATAT GAAAGACAAG TTGCTGATAT TGAGATTGTT 
GAAAAAGAAG ATCTTGATCT TAAAAATCAT CTATCTGGCC TGCAAAAAAA ATACCTAAAA 
ATATCATTTG ATACTGTGCA ACAATTGATG GAAGTCAAGC GGGATTTGTT ACATATTGTC 
GAACGAAACC AGGCAAAATT TGATGCACTT GAAGCATACG AATCCATATT GGCTGGAAAA 
CGAGAACAAC GTCCTCAAGA TTGCTTAGAC TCTATCGTGG ACCTCCGCGA GTACGATGTA 
CCTTATCATG TTCGATTTGC AATTGATAAT GATGTGCGGA GTGGGCAATG GTACAATGTC 
AGTATTTCCA GCACCGATGT CATATTGGAG AAGAGGACAG ATCTTCTTCA ACGCGCAGAA 
GTCCGTGTTT GTGCCTTCGA TATAGAGACA ACAAAGCTTC CGTTGAAGTT TCCTGATGCT 
GAATATGATC AAATTATGAT GATATCCTAC ATGGTCGATG GGCAAGGTTT TCTAATTATT 
AACAGAGAGT GTGTTGGCGA AGATGTAGAA GATCTGGAAT ATACACCAAA ACCAGAATTT 
GAAGGGTATT TCAAAGTTAC TAATGTTAAA AATGAGGTTG AACTCCTCCA AAGATGGTTT 
TACCATATGC AAGAGTTGAA ACCCGGTATT TATGTTACAT ACAACGGTGA CTTTTTTGAC 
TGGCCATTTA TAGAACGGAG AGCATCTCAT CATGGGATAA AAATGAATGA GGAATTGGGA 
TTCCGTTGTG ATCAGAATCA AGGTGAATGT CGAGCTAAAT TCGCTTGTCA TTTGGATTGT 
TTTGCTTGGG TTAAACGTGA TAGCTATCTT CCTCAGGGAA GCCATGGTCT GAAGGCTGTT 
ACAAAGGCAA AACTAGGTTA TGATCCACTG GAAGTGAACC CAGAGGACAT GGTTCGGTTT 
GCGATGGAAA AGCCCCAGAC AATGGCCTCC TACTCTGTCT CTGATGCTGT TGCAACTTAT 
TACCTATACA TGACATATGT CAATCCATTC ATTTTTTCTC TCGCAACTAT CATCCCAATG 
GTCCCTGATG AGGTTTTACG TAAAGGGAGT GGCACCCTTT GTGAAATGCT TCTGATGGTT 
GAGGCTTACA AGGCAAATGT TGTATGTCCC AACAAAAATC AAGCTGACCC TGAGAAATTC 
TACCAAAATC AACTTCTTGA AAGTGAGACA TATATTGGTG GTCATGTAGA GTGCCTTGAA 
AGTGGTGTTT TCAGATCTGA CATTCCAACC AGTTTTAAAC TCGATTCATC GGCATACCAG 
CAACTGATCG ATAACCTTGG TCGTGATCTG GAATATGCTA TCACTGTAGA AGGAAAAATG 
AGAATGGATT CAATTTCCAA TTATGATGAA GTGAAGGACG AAATCAAGGA GAAGCTTGAG 
AAGTTACGAG ATGACCCTAT ACGGGAAGAG GGGCCTCTTA TTTATCACCT TGATGTCGCA 
GCCATGTATC CAAATATCAT CTTAACAAAC AGGCTTCAGC CACCATCAAT AGTTACGGAT 
GAGATTTGCA CAGCATGTGA TTTCAACCGC CCGGGAAAGA CCTGTCTTAG AAAACTTGAA 
TGGGTTTGGC GTGGTGTGAC ATTCATGGGA AAGAAAAGTG ATTATTACCA TTTGAAGAAG 
CAGATTGAGT CTGAATTTGT TGATGCTGGT GCAAATATAA TGTCTTCAAA ATCGTTCCTT 
GATCTGCCAA AGGTGGATCA ACAATCTAAG CTAAAAGAAC GACTAAAAAA ATATTGTCAA 
AAGGCTTATA AACGAGTACT TGACAAGCCA ATTACTGAAG TTCGTGAAGC TGGGATATGC 
ATGAGAGAAA ACCCTTTCTA TGTGGATACT GTTCGGAGCT TTCGAGATAG GAGGTATGAA 
TACAAAACAC TTAACAAGGT CTGGAAGGGA AAATTGTCAG AGGCCAAGGC AAGTGGTAAT 
TCAATCAAGA TCCAGGAAGC ACAGGACATG GTAGTGGTTT ACGATTCCTT GCAGCTAGCT 
CATAAGTGTA TACTTAATTC CTTCTATGGA TATGTCATGC GAAAGGGTGC AAGATGGTAT 
TCCATGGAAA TGGCTGGTGT AGTTACCTAT ACTGGAGCCA AAATCATACA GAATGCCCGT 
TTGCTTATTG AGCGAATTGG GAAACCACTT GAGTTGGATA CAGATGGTAT ATGGTGTTGT 
CTCCCTGGAT CTTTTCCTGA GAACTTTACT TTTAAAACAA TAGACATGAA GAAACTCACC 
ATCTCGTATC CTTGTGTTAT GCTTAATGTT GATGTGGCAA AGAATAATAC AAATGATCAA 
TATCAGACTC TTGTAGATCC TGTACGCAAG ACATATAAAT CACATAGTGA ATGCTCAATT 
GAATTTGAAG TAGATGGACC GTACAAGGCA ATGATTATTC CTGCATCGAA AGAAGAAGGA 
ATCTTAATCA AGAAGCGTTA TGCTGTTTTC AATCATGATG GAACCTTAGC AGAACTCAAG 
GGGTTTGAGA TCAAGCGTAG AGGGGAGCTG AAACTCATTA AAGTTTTCCA GGCCGAGCTT 
TTTGACAAAT TTCTCCATGG ATCAACTCTT GAAGAGTGTT ATTCTGCTGT TGCAGCGGTT 
GCTGATCGAT GGCTTGACCT ACTCGATAAT CAAGGAAAAG ATATTGCCGA TAGTGAACTG 
CTAGATTATA TATCAGAATC AAGCACAATG AGCAAATCGT TAGCAGATTA TGGTGAGCAA 
AAGTCATGTG CAGTGACCAC AGCAAAACGC CTGGCTGAAT TTCTTGGTGT TACAATGGTC 
AAAGATAAAG GACTACGTTG CCAGTACATT GTTGCTTGTG AACCAAAGGG TACACCGGTA 
AGCGAACGTG CTGTTCCTGT TGCCATATTT ACAACAAATC CGGAAGTTAT GAAGTTTCAT 
TTGCGAAAAT GGTGCAAGAC ATCATCAGAT GTCGGAATTC GTCTGATTAT TGACTGGTCG 
TATTACAAGC AGCGCCTTAG TTCAGCTATC CAAAAAGTTA TTACCATTCC TGCTGCAATG 
CAGAAGGTGG CAAATCCCGT GCCTAGGGTG CTTCATCCTG ACTGGCTCCA TAAAAAGGTC 
CGTGAGAAAG ACGACAAATT TCGCCAGAGA AAACTAGTTG ATATGTTCAG CTCAGCAAAT 
AAAGATGTTG TGTTGGACAC AGATCTTCCG GTGACTAAAG ATAATGTGGA AGATATTGAG 
GATTTTTGCA AAGAAAATAG GCCTTCCGTC AAAGGGCCAA AGCCAATTGC GCGTTCCTAT 
GAAGTCAATA AAAAACAGTC TGAATGTGAG CAGCAAGAGA GCTGGGATAC AGAGTTCCAT 
GATATCTCAT TTCAGAACAT TGACAAGAGC GTAAACTACC AAGGATGGCT TGAACTGAAA 
AAGAGAAAAT GGAAAGTAAC TCTAGAGAAG AAGAAAAAAC GAAGGTTGGG TGATCTAAGA 
TCTTCAAACC AGGTTGATAC TCACGAGATA AATCAAAAAG TCGGTCAGGG AAGAGGAGGT 
GTGGGTTCAT ACTTTAGGAG GCCTGAAGAA GCTTTGACTA GTTCACATTG GCAGATAATA 
CAGCTGGTCC CAAGTCCACA GAGCGGACAA TTTTTTGCTT GGGTGGTTGT GGAAGGGTTG 
ATGCTTAAGA TCCCATTGTC GATCCCAAGG GTGTTTTACA TTAATTCCAA AGTTCCTATA 
GATGAATATT TCCAAGGAAA GTGTGTCAAC AAGATTCTTC CTCATGGAAG GCCTTGTTAT 
AGTTTGACTG AGGTCAAGAT TCAAGAAGAT CAATTTAAAA AAGAAAGTAA AAAGCGTGCA 
GCTCTTCTTG CAGACCCTGG AGTTGAGGGC ATATATGAAA CTAAGGTGCC TCTGGAGTTT 
AGTGCTATAT GTCAGATTGG ATGTGTATGC AAAATTGATA ACAAAGCCAA GCACCGTAAC 
ACCCAAGATG GATGGGAAGT TGGTGAACTT CACATGAAAA CTACAACTGA GTGTCACTAC 
TTGAAGCGTT CAATTCCACT TGTTTACTTG TATAACAGCA CCTCAACAGG GCGTGCTATA 
TATGTTTTAT ATTGTCATGT ATCGAAGCTT ATGTCTGCCG TTGTAGTTGA TCCTTTTAAC 
GGCAATGAAT TATTACCATC TGCCTTAGAG AGACAGTTTA GAGATAGTTG CCTAGAATTA 
TCACTTGATT CATTGTCTTG GGATGGTATC CGTTTCCAGG TGCACTACGT TGACCATCCC 
GAAGCTGCCA AGAAGATAAT ACAAAGAGCA ATAAGTGAAT ACAGAGAAGA AAATTGTGGA 
CCAACTGTTG CTGTCATTGA ATGCCCTGAC TTTACCTTCA TGAAGGAAGG TATTAAGGCC 
CTAGATGATT TCCCATGTGT GAGGATTCCC TTCAACGACG ATGACAATAG TTATCAGCCT 
GTCTCCTGGC AACGTCCAGC TGCAAAAATA GCAATGTTCC GTTGTGCTGC AGCATTTCAG 
TGGTTGGATC GGAGGATTAC CCAATCAAGA TACGCCCATG TGCCTCTGGG GAATTTTGGA 
CTTGATTGGC TAACATTCAC AATAGATATC TTCCTATCCA GGGCACTCCG TGACCAGCAA 
CAGGTCCTTT GGGTGTCAGA TAATGGTGTT CCAGACCTTG GAGGTATAAA CAATGAAGAG 
GCATTCTTTG CCGATGAGGT GCAACAGACA AGTTTAGTAT TCCCTGGAGC GTATAGAAAA 
GTGTCTGTGG AGTTAAAGAT CCATAACTTG GCTGTTAATG CCCTTCTGAA AAGCAACCTG 
GTGAATGAAA TGGAAGGAGG CGGTTTTATG GGATTTGAAC AGGATGTGAA TCCTAGAGGA 
ATTAATTCAA ACGATAACAC CAGTTTTGAT GAGACCACTG GATGTGCTCA AGCATTTCGT 
GTCCTGAAAC AGTTGATTCA TAGTTGCTTG ACTGATGTGC GTAAATCTAA AAATATATAT 
GCTGATTCCA TTTTGCAACG TTTGTCTTGG TGGCTCTGCA GCCCTTCCTC GAAGCTCCAT 
GATCCAGCTC TTCATCTCAT GCTTCACAAG GTCATGCAAA AGGTGTTTGC ACTGCTATTG 
ACTGATTTGC GGAGATTAGG TGCTATAATA ATATATGCAG ACTTCTCAAA GGTCATTATC 
GACACAGTGA AATTTGATCT ATCTGCAGCT AAGGCTTATT GTGAAAGCTT GCTATCAACG 
GTGCGGAATA GTGATATCTT TGAGTGGATA TTGCTCGAGC CGGTTCACTA CTGGCACTCA 
TTACTTTTCA TGGACCAGTA TAACTACGCT GGTATCCGAG CCGATGATGA AATTTCACTT 
GACGAAGTGA CTATTGAACC AAAGTGGAGT GTTGCACGAC ATTTACCTGA GTATATTGAG 
AGGGATTTCA TTATAATCAT TGCCAAGTTT ATATTTGACC CCTGGAAATT TGCGATAGAA 
AATAAAAAGG GCAGTTCAGA GAGTTTAGAG GCACAGATGA TAGAATATCT AAGAGAACAG 
ATTGGATCAA CCTTCATAAA TATGCTTGTC AAAAAGGTTG ATGATATCAT GTCACACATG 
AAGGAGATAA ATGTTTCAGA TGCATCTCGG GTTTCTGGTC AAGCTCCCAA AGGAGATTAC 
TCGTTGGAAT TTATACAGGT TATCTCTGCT GTTCTGGCTC TTGACCAAAA CGTCCAGCAA 
GATGTTCTGG TAATGAGAAA GAGTCTGTTG AAGTACATTA AAGTAAAAGA GTGTGCTGCT 
GAAGCCGAAT TTCTTGATCC TGGGCCATCC TTCATCTTAC CTAATGTAGC TTGCAGCAAC 
TGTGATGCCT ACAGAGACCT AGATATATGC AGAGACCCAG CACTGTTAAC AGAGAAAGAA 
TGGTCATGTG CGGATACACA ATGTGGGAAG ATATATGACA GAGAGCAGAT GGAGAGTAGC 
CTTCTAGAGA TGGTGAGACA GAGAGAAAGA ATGTACCATA TGCAGGATGT AGTGTGCATT 
AGGTGCAATC AGGTGAAAGC GGCTCATTTG ACAGAGCAGT GTGAGTGTTC TGGTTCCTTT 
AGATGCAAGG AGAGTGGGTC AGAGTTCAGC AAGAGGATGG AGATATTCAT GGATATAGCA 
AAGCGCCAGA AGTTTAGACT GTTAGAAGAA TACATCTCTT GGATCATATA TGGCCCTAGC 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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TIL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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