ube3a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ube3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24653 NM_001001213.1
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa26250 XM_012956687.2
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa42494 XM_018091154.1
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa51257 XM_012956687.3
Latest version!
Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24653
    Clone ID Related Accession (Same CDS sequence) NM_001001213.1
    Accession Version NM_001001213.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067999.1. ##Evidence-Data-START## Transcript exon combination :: BC067999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGT 
    TGTGGAAATG AAACCTGCAC GAATGAATTT TGTGCTTCCT GTCCCACTTT TCTTCGTATG
    GATAACAATG CAGCAGCCAT TAAGGCCCTC GAGTTATATA AGGTTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAACAAGC TCATCTTATC TAGAGAACAA TTCGAAAAGT
    GCCCATAACA ACTCCTGCAC AGAAAGAAAG ATGAATAAGA AAGACCTTCA TGGACCCAGA
    GATGACTTTA AAGATGTCAC TTTCCTGTCT GAAGAAAAAG TGTATGAAAT TCTTGAATTG
    TGTAGAGAAA AAGAGGACTA CTCGCCTTTA ATTCGGGTGA TCGGGAGAGT ATTTTCCAGT
    GCAGAAGCTT TGGTTCAGAG TTTCCGAAAA GCAAAGCACC ATACAAAGGA GGAGCTAAAA
    TCCTTGCAAG AAAAGGATGA AGATAAGGAT GAAGATGAAA AGGAAAAAGC TGCATCATCT
    GCTGCTGCAA TGGAAGAGGA TTCTGGAGCA TCATCCTCAA GGTTAAGTGA CAACTCACAA
    GGAGACAACA ACATACAGAA ACTAGGCCCT GAAGAAGTGT CTCTAGATAT AGAAGCAGTT
    CGACGGGTGT ATCACAGGTT ACTTTCTAAT GAGAAAATAG AAACTGCCTT TCTGAATGCA
    CTTGTGTACC TTTCTCCTAA TGTGGAATGT GACTTAACTT ATCATAATGT TTATTCACGA
    GATCCCAACT ATCTCAATTT GTTTATAATC GTCATGGAGA ATGGTAATCT CCATAGTCCT
    GAATACCTGG AAATGGCTTT ACCGTTGTTT TGCAAAGCAA TGAGTAAATT GCCATTAGCA
    GCGCAAGCAA AGTTGATCCG TCTTTGGTCA AAGTACAGCG CTGAGCAGAT TCGCCGAATG
    ATGGAAACAT TTCAGCAGCT TATAACCTAT AAAGTTATAA GTAACGAGTT CAACAGTCGA
    AATTTAGTCA ATGATGATGA TGCTATTGTT GCAGCAACAA AGTGCTTAAA AATGGTTTAC
    TATTCAAATG TTGTTGGTGG AGAGATTGAA ACTGACCATA ATGAAGAGGA AGATGATGAA
    CCTGTTCCTG AGTCCAGTGA GTTAACTCTG CAGGAGCTGC TAGGGGAAGA ACGAAGAAAC
    AAAAAAGGCC CTCGAGTAGA TCCCCTTGAA ACCGAACTTG GTGTTAAAAG CATTGATTGC
    AGGAAACCTC TCATCCCTTT CGAGGAATTC GTTAACGAAC CACTAAATGA TGTTCTAGAA
    ATGGACAAAG ATTATACTTT CTTTAAAGTA GAGACTGAAA ACAAATTCTC TTTTATGACT
    TGCCCCTTTA TCCTGAATGC AGTTACAAAG AATCTTGGTC TGTATTATGA CAACAGAATC
    CGCATGTACA GTGAAAGGCG AATAACTGTT CTGTATAGTC TAGTGCAAGG ACAGCAACTT
    AACCCCTATT TGAGGCTCAA AGTACGGCGA GATCACATCA TTGACGATGC TCTTGTTAGG
    CTTGAGATGA TTGCCATGGA AAATCCTGCA GATTTGAAGA AGCAATTATA TGTGGAATTT
    GAGGGAGAAC AAGGAGTTGA CGAAGGAGGT GTTTCTAAAG AGTTTTTCCA ATTAGTTGTT
    GAAGAAATTT TCAACCCTGA TATTGGTATG TTTACGTATG ATGAGTCCAC TAAACACTCT
    TGGTTCAATC CATCATCTTT TGAGACAGAA GGACAGTTTA CACTTATTGG CATTGTGCTG
    GGCCTTGCAA TTTACAATAA TTGCATTCTA GATGTGCATT TTCCAATGGT TGTATATAGA
    AAACTAATGG GGAAAAAAGG AACCTTCCGT GACCTTGCTG ATTCTCATCC TGTTCTTTAT
    CAGAGTTTAA AAGAATTGTT GGAGTATGAA GGCAATGTTG AAGAAGATAT GATGATGACT
    TTCCAGATCT CTCAGACTGA CCTCTTTGGA AATCCTTTAA TGCACGACTT AAAGGAAAAT
    GGAGATAAAA TCCCCATTAC AAATGAGAAC AGAAAGGAAT TTGTCAGTCT ATACACAGAC
    TATATTCTAA ATAAATCAGT GGAAAAGCAA TTTAAAGCCT TCAGAAGGGG CTTTCACATG
    GTTACCAATG AATCGCCTCT GAAATATCTG TTTAGACCAG AGGAGATTGA ACTTCTTATT
    TGTGGAAGTA GGAATTTAGA TTTCCAAGCT CTGAAAGATA CTACGGAATA TGATGGTGGC
    TATACCAGAG ATTCAAATAT AATAAAGGAG TTTTGGGAAA TTGTAAATTC TTTCACAGAG
    GAACAAAAAA GATTGTTCTT GCAATTTACA ACAGGCACGG ACAGAGCACC TGTAGGTGGG
    CTAGGCAAAT TAAAGATGAT TATAGCCAAA AACGGCCCTG ATACAGACAG GTTACCAACA
    TCTCATACTT GCTTTAATGT CCTTTTGCTT CCGGAGTACT CAAATAAAGA GAAACTGAAG
    GAGAGATTGT TAAAGGCCAT CACTTATGCT AAGGGGTTTG GTATGCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001001213.1
    CDS334..2904
    Translation

    Target ORF information:

    RefSeq Version NM_001001213.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001001213.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGT 
    TGTGGAAATG AAACCTGCAC GAATGAATTT TGTGCTTCCT GTCCCACTTT TCTTCGTATG
    GATAACAATG CAGCAGCCAT TAAGGCCCTC GAGTTATATA AGGTTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAACAAGC TCATCTTATC TAGAGAACAA TTCGAAAAGT
    GCCCATAACA ACTCCTGCAC AGAAAGAAAG ATGAATAAGA AAGACCTTCA TGGACCCAGA
    GATGACTTTA AAGATGTCAC TTTCCTGTCT GAAGAAAAAG TGTATGAAAT TCTTGAATTG
    TGTAGAGAAA AAGAGGACTA CTCGCCTTTA ATTCGGGTGA TCGGGAGAGT ATTTTCCAGT
    GCAGAAGCTT TGGTTCAGAG TTTCCGAAAA GCAAAGCACC ATACAAAGGA GGAGCTAAAA
    TCCTTGCAAG AAAAGGATGA AGATAAGGAT GAAGATGAAA AGGAAAAAGC TGCATCATCT
    GCTGCTGCAA TGGAAGAGGA TTCTGGAGCA TCATCCTCAA GGTTAAGTGA CAACTCACAA
    GGAGACAACA ACATACAGAA ACTAGGCCCT GAAGAAGTGT CTCTAGATAT AGAAGCAGTT
    CGACGGGTGT ATCACAGGTT ACTTTCTAAT GAGAAAATAG AAACTGCCTT TCTGAATGCA
    CTTGTGTACC TTTCTCCTAA TGTGGAATGT GACTTAACTT ATCATAATGT TTATTCACGA
    GATCCCAACT ATCTCAATTT GTTTATAATC GTCATGGAGA ATGGTAATCT CCATAGTCCT
    GAATACCTGG AAATGGCTTT ACCGTTGTTT TGCAAAGCAA TGAGTAAATT GCCATTAGCA
    GCGCAAGCAA AGTTGATCCG TCTTTGGTCA AAGTACAGCG CTGAGCAGAT TCGCCGAATG
    ATGGAAACAT TTCAGCAGCT TATAACCTAT AAAGTTATAA GTAACGAGTT CAACAGTCGA
    AATTTAGTCA ATGATGATGA TGCTATTGTT GCAGCAACAA AGTGCTTAAA AATGGTTTAC
    TATTCAAATG TTGTTGGTGG AGAGATTGAA ACTGACCATA ATGAAGAGGA AGATGATGAA
    CCTGTTCCTG AGTCCAGTGA GTTAACTCTG CAGGAGCTGC TAGGGGAAGA ACGAAGAAAC
    AAAAAAGGCC CTCGAGTAGA TCCCCTTGAA ACCGAACTTG GTGTTAAAAG CATTGATTGC
    AGGAAACCTC TCATCCCTTT CGAGGAATTC GTTAACGAAC CACTAAATGA TGTTCTAGAA
    ATGGACAAAG ATTATACTTT CTTTAAAGTA GAGACTGAAA ACAAATTCTC TTTTATGACT
    TGCCCCTTTA TCCTGAATGC AGTTACAAAG AATCTTGGTC TGTATTATGA CAACAGAATC
    CGCATGTACA GTGAAAGGCG AATAACTGTT CTGTATAGTC TAGTGCAAGG ACAGCAACTT
    AACCCCTATT TGAGGCTCAA AGTACGGCGA GATCACATCA TTGACGATGC TCTTGTTAGG
    CTTGAGATGA TTGCCATGGA AAATCCTGCA GATTTGAAGA AGCAATTATA TGTGGAATTT
    GAGGGAGAAC AAGGAGTTGA CGAAGGAGGT GTTTCTAAAG AGTTTTTCCA ATTAGTTGTT
    GAAGAAATTT TCAACCCTGA TATTGGTATG TTTACGTATG ATGAGTCCAC TAAACACTCT
    TGGTTCAATC CATCATCTTT TGAGACAGAA GGACAGTTTA CACTTATTGG CATTGTGCTG
    GGCCTTGCAA TTTACAATAA TTGCATTCTA GATGTGCATT TTCCAATGGT TGTATATAGA
    AAACTAATGG GGAAAAAAGG AACCTTCCGT GACCTTGCTG ATTCTCATCC TGTTCTTTAT
    CAGAGTTTAA AAGAATTGTT GGAGTATGAA GGCAATGTTG AAGAAGATAT GATGATGACT
    TTCCAGATCT CTCAGACTGA CCTCTTTGGA AATCCTTTAA TGCACGACTT AAAGGAAAAT
    GGAGATAAAA TCCCCATTAC AAATGAGAAC AGAAAGGAAT TTGTCAGTCT ATACACAGAC
    TATATTCTAA ATAAATCAGT GGAAAAGCAA TTTAAAGCCT TCAGAAGGGG CTTTCACATG
    GTTACCAATG AATCGCCTCT GAAATATCTG TTTAGACCAG AGGAGATTGA ACTTCTTATT
    TGTGGAAGTA GGAATTTAGA TTTCCAAGCT CTGAAAGATA CTACGGAATA TGATGGTGGC
    TATACCAGAG ATTCAAATAT AATAAAGGAG TTTTGGGAAA TTGTAAATTC TTTCACAGAG
    GAACAAAAAA GATTGTTCTT GCAATTTACA ACAGGCACGG ACAGAGCACC TGTAGGTGGG
    CTAGGCAAAT TAAAGATGAT TATAGCCAAA AACGGCCCTG ATACAGACAG GTTACCAACA
    TCTCATACTT GCTTTAATGT CCTTTTGCTT CCGGAGTACT CAAATAAAGA GAAACTGAAG
    GAGAGATTGT TAAAGGCCAT CACTTATGCT AAGGGGTTTG GTATGCTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa26250
    Clone ID Related Accession (Same CDS sequence) XM_012956687.2
    Accession Version XM_012956687.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
    AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA
    GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT
    CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT
    GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG
    GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA
    ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA
    AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC
    CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG
    CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC
    CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT
    AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT
    GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA
    GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT
    TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG
    GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT
    ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG
    TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT
    TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA
    ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTAGAAATT TAGTCAATGA TGATGATGCT
    ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG
    ATTGAAACTG ACCATAATGA AGAGGAAGAC GATGAACCTG TTCCTGAGTC CAGTGAGTTA
    ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC
    CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG
    GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT
    AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT
    ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA
    ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA
    CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT
    CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA
    GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT
    GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG
    ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC
    ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC
    TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG
    TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC
    TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT
    GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA
    AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA
    TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC
    CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA
    AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA
    TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA
    GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT
    TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT
    TATGCTAAGG GGTTTGGTAT GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812141.1
    CDS194..2920
    Translation

    Target ORF information:

    RefSeq Version XM_012956687.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956687.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
    AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA
    GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT
    CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT
    GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG
    GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA
    ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA
    AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC
    CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG
    CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC
    CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT
    AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT
    GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA
    GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT
    TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG
    GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT
    ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG
    TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT
    TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA
    ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTAGAAATT TAGTCAATGA TGATGATGCT
    ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG
    ATTGAAACTG ACCATAATGA AGAGGAAGAC GATGAACCTG TTCCTGAGTC CAGTGAGTTA
    ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC
    CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG
    GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT
    AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT
    ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA
    ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA
    CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT
    CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA
    GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT
    GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG
    ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC
    ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC
    TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG
    TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC
    TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT
    GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA
    AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA
    TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC
    CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA
    AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA
    TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA
    GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT
    TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT
    TATGCTAAGG GGTTTGGTAT GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42494
    Clone ID Related Accession (Same CDS sequence) XM_018091154.1
    Accession Version XM_018091154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
    AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGGAAGCGA
    GCAGCTGCAA AGCATCTAAT AGAACGCTAC TACCACCAGT TAACTGAGGG TTGTGGAAAT
    GAAACCTGCA CGAATGAATT TTGTGCTTCC TGTCCCACTT TTCTTCGTAT GGATAACAAT
    GCAGCAGCCA TTAAGGCCCT CGAGTTATAT AAGGTTAATG CAAAACTCTG TGATCCTCAT
    CCCTCCAAGA AAGGAACAAG CTCATCTTAT CTAGAGAACA ATTCGAAAAG TGCCCATAAC
    AACTCCTGCA CAGAAAGAAA GATGAATAAG AAAGACCTTC ATGGACCCAG AGATGACTTT
    AAAGATGTCA CTTTCCTGTC TGAAGAAAAA GTGTATGAAA TTCTTGAATT GTGTAGAGAA
    AAAGAGGACT ACTCGCCTTT AATTCGGGTG ATCGGGAGAG TATTTTCCAG TGCAGAAGCT
    TTGGTTCAGA GTTTCCGAAA AGCAAAGCAC CATACAAAGG AGGAGCTAAA ATCCTTGCAA
    GAAAAGGATG AAGATAAGGA TGAAGATGAA AAGGAAAAAG CTGCATCATC TGCTGCTGCA
    ATGGAAGAGG ATTCTGGAGC ATCATCCTCA AGGTTAAGTG ACAACTCACA AGGAGACAAC
    AACATACAGA AACTAGGCCC TGAAGAAGTG TCTCTAGATA TAGAAGCAGT TCGACGGGTG
    TATCACAGGT TACTTTCTAA TGAGAAAATA GAAACTGCCT TTCTGAATGC ACTTGTGTAC
    CTTTCTCCTA ATGTGGAATG TGACTTAACT TATCATAATG TTTATTCACG AGATCCCAAC
    TATCTCAATT TGTTTATAAT CGTCATGGAG AATGGTAATC TCCATAGTCC TGAATACCTG
    GAAATGGCTT TACCGTTGTT TTGCAAAGCA ATGAGTAAAT TGCCATTAGC AGCGCAAGCA
    AAGTTGATCC GTCTTTGGTC AAAGTACAGC GCTGAGCAGA TTCGCCGAAT GATGGAAACA
    TTTCAGCAGC TTATAACCTA TAAAGTTATA AGTAACGAGT TCAACAGTAG AAATTTAGTC
    AATGATGATG ATGCTATTGT TGCAGCAACA AAGTGCTTAA AAATGGTTTA CTATTCAAAT
    GTTGTTGGTG GAGAGATTGA AACTGACCAT AATGAAGAGG AAGACGATGA ACCTGTTCCT
    GAGTCCAGTG AGTTAACTCT GCAGGAGCTG CTAGGGGAAG AACGAAGAAA CAAAAAAGGC
    CCTCGAGTAG ATCCCCTTGA AACCGAACTT GGTGTTAAAA GCATTGATTG CAGGAAACCT
    CTCATCCCTT TCGAGGAATT CGTTAACGAA CCACTAAATG ATGTTCTAGA AATGGACAAA
    GATTATACTT TCTTTAAAGT AGAGACTGAA AACAAATTCT CTTTTATGAC TTGCCCCTTT
    ATCCTGAATG CAGTTACAAA GAATCTTGGT CTGTATTATG ACAACAGAAT CCGCATGTAC
    AGTGAAAGGC GAATAACTGT TCTGTATAGT CTAGTGCAAG GACAGCAACT TAACCCCTAT
    TTGAGGCTCA AAGTACGGCG AGATCACATC ATTGACGATG CTCTTGTTAG GCTTGAGATG
    ATTGCCATGG AAAATCCTGC AGATTTGAAG AAGCAATTAT ATGTGGAATT TGAGGGAGAA
    CAAGGAGTTG ACGAAGGAGG TGTTTCTAAA GAGTTTTTCC AATTAGTTGT TGAAGAAATT
    TTCAACCCTG ATATTGGTAT GTTTACGTAT GATGAGTCCA CTAAACACTC TTGGTTCAAT
    CCATCATCTT TTGAGACAGA AGGACAGTTT ACACTTATTG GCATTGTGCT GGGCCTTGCA
    ATTTACAATA ATTGCATTCT AGATGTGCAT TTTCCAATGG TTGTATATAG AAAACTAATG
    GGGAAAAAAG GAACCTTCCG TGACCTTGCT GATTCTCATC CTGTTCTTTA TCAGAGTTTA
    AAAGAATTGT TGGAGTATGA AGGCAATGTT GAAGAAGATA TGATGATGAC TTTCCAGATC
    TCTCAGACTG ACCTCTTTGG AAATCCTTTA ATGCACGACT TAAAGGAAAA TGGAGATAAA
    ATCCCCATTA CAAATGAGAA CAGAAAGGAA TTTGTCAGTC TATACACAGA CTATATTCTA
    AATAAATCAG TGGAAAAGCA ATTTAAAGCC TTCAGAAGGG GCTTTCACAT GGTTACCAAT
    GAATCGCCTC TGAAATATCT GTTTAGACCA GAGGAGATTG AACTTCTTAT TTGTGGAAGT
    AGGAATTTAG ATTTCCAAGC TCTGAAAGAT ACTACGGAAT ATGATGGTGG CTATACCAGA
    GATTCAAATA TAATAAAGGA GTTTTGGGAA ATTGTAAATT CTTTCACAGA GGAACAAAAA
    AGATTGTTCT TGCAATTTAC AACAGGCACG GACAGAGCAC CTGTAGGTGG GCTAGGCAAA
    TTAAAGATGA TTATAGCCAA AAACGGCCCT GATACAGACA GGTTACCAAC ATCTCATACT
    TGCTTTAATG TCCTTTTGCT TCCGGAGTAC TCAAATAAAG AGAAACTGAA GGAGAGATTG
    TTAAAGGCCA TCACTTATGC TAAGGGGTTT GGTATGCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946643.1
    CDS195..2876
    Translation

    Target ORF information:

    RefSeq Version XM_018091154.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
    AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGGAAGCGA
    GCAGCTGCAA AGCATCTAAT AGAACGCTAC TACCACCAGT TAACTGAGGG TTGTGGAAAT
    GAAACCTGCA CGAATGAATT TTGTGCTTCC TGTCCCACTT TTCTTCGTAT GGATAACAAT
    GCAGCAGCCA TTAAGGCCCT CGAGTTATAT AAGGTTAATG CAAAACTCTG TGATCCTCAT
    CCCTCCAAGA AAGGAACAAG CTCATCTTAT CTAGAGAACA ATTCGAAAAG TGCCCATAAC
    AACTCCTGCA CAGAAAGAAA GATGAATAAG AAAGACCTTC ATGGACCCAG AGATGACTTT
    AAAGATGTCA CTTTCCTGTC TGAAGAAAAA GTGTATGAAA TTCTTGAATT GTGTAGAGAA
    AAAGAGGACT ACTCGCCTTT AATTCGGGTG ATCGGGAGAG TATTTTCCAG TGCAGAAGCT
    TTGGTTCAGA GTTTCCGAAA AGCAAAGCAC CATACAAAGG AGGAGCTAAA ATCCTTGCAA
    GAAAAGGATG AAGATAAGGA TGAAGATGAA AAGGAAAAAG CTGCATCATC TGCTGCTGCA
    ATGGAAGAGG ATTCTGGAGC ATCATCCTCA AGGTTAAGTG ACAACTCACA AGGAGACAAC
    AACATACAGA AACTAGGCCC TGAAGAAGTG TCTCTAGATA TAGAAGCAGT TCGACGGGTG
    TATCACAGGT TACTTTCTAA TGAGAAAATA GAAACTGCCT TTCTGAATGC ACTTGTGTAC
    CTTTCTCCTA ATGTGGAATG TGACTTAACT TATCATAATG TTTATTCACG AGATCCCAAC
    TATCTCAATT TGTTTATAAT CGTCATGGAG AATGGTAATC TCCATAGTCC TGAATACCTG
    GAAATGGCTT TACCGTTGTT TTGCAAAGCA ATGAGTAAAT TGCCATTAGC AGCGCAAGCA
    AAGTTGATCC GTCTTTGGTC AAAGTACAGC GCTGAGCAGA TTCGCCGAAT GATGGAAACA
    TTTCAGCAGC TTATAACCTA TAAAGTTATA AGTAACGAGT TCAACAGTAG AAATTTAGTC
    AATGATGATG ATGCTATTGT TGCAGCAACA AAGTGCTTAA AAATGGTTTA CTATTCAAAT
    GTTGTTGGTG GAGAGATTGA AACTGACCAT AATGAAGAGG AAGACGATGA ACCTGTTCCT
    GAGTCCAGTG AGTTAACTCT GCAGGAGCTG CTAGGGGAAG AACGAAGAAA CAAAAAAGGC
    CCTCGAGTAG ATCCCCTTGA AACCGAACTT GGTGTTAAAA GCATTGATTG CAGGAAACCT
    CTCATCCCTT TCGAGGAATT CGTTAACGAA CCACTAAATG ATGTTCTAGA AATGGACAAA
    GATTATACTT TCTTTAAAGT AGAGACTGAA AACAAATTCT CTTTTATGAC TTGCCCCTTT
    ATCCTGAATG CAGTTACAAA GAATCTTGGT CTGTATTATG ACAACAGAAT CCGCATGTAC
    AGTGAAAGGC GAATAACTGT TCTGTATAGT CTAGTGCAAG GACAGCAACT TAACCCCTAT
    TTGAGGCTCA AAGTACGGCG AGATCACATC ATTGACGATG CTCTTGTTAG GCTTGAGATG
    ATTGCCATGG AAAATCCTGC AGATTTGAAG AAGCAATTAT ATGTGGAATT TGAGGGAGAA
    CAAGGAGTTG ACGAAGGAGG TGTTTCTAAA GAGTTTTTCC AATTAGTTGT TGAAGAAATT
    TTCAACCCTG ATATTGGTAT GTTTACGTAT GATGAGTCCA CTAAACACTC TTGGTTCAAT
    CCATCATCTT TTGAGACAGA AGGACAGTTT ACACTTATTG GCATTGTGCT GGGCCTTGCA
    ATTTACAATA ATTGCATTCT AGATGTGCAT TTTCCAATGG TTGTATATAG AAAACTAATG
    GGGAAAAAAG GAACCTTCCG TGACCTTGCT GATTCTCATC CTGTTCTTTA TCAGAGTTTA
    AAAGAATTGT TGGAGTATGA AGGCAATGTT GAAGAAGATA TGATGATGAC TTTCCAGATC
    TCTCAGACTG ACCTCTTTGG AAATCCTTTA ATGCACGACT TAAAGGAAAA TGGAGATAAA
    ATCCCCATTA CAAATGAGAA CAGAAAGGAA TTTGTCAGTC TATACACAGA CTATATTCTA
    AATAAATCAG TGGAAAAGCA ATTTAAAGCC TTCAGAAGGG GCTTTCACAT GGTTACCAAT
    GAATCGCCTC TGAAATATCT GTTTAGACCA GAGGAGATTG AACTTCTTAT TTGTGGAAGT
    AGGAATTTAG ATTTCCAAGC TCTGAAAGAT ACTACGGAAT ATGATGGTGG CTATACCAGA
    GATTCAAATA TAATAAAGGA GTTTTGGGAA ATTGTAAATT CTTTCACAGA GGAACAAAAA
    AGATTGTTCT TGCAATTTAC AACAGGCACG GACAGAGCAC CTGTAGGTGG GCTAGGCAAA
    TTAAAGATGA TTATAGCCAA AAACGGCCCT GATACAGACA GGTTACCAAC ATCTCATACT
    TGCTTTAATG TCCTTTTGCT TCCGGAGTAC TCAAATAAAG AGAAACTGAA GGAGAGATTG
    TTAAAGGCCA TCACTTATGC TAAGGGGTTT GGTATGCTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51257
    Clone ID Related Accession (Same CDS sequence) XM_012956687.3
    Accession Version XM_012956687.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
    AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA
    GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT
    CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT
    GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG
    GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA
    ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA
    AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC
    CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG
    CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC
    CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT
    AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT
    GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA
    GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT
    TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG
    GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT
    ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG
    TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT
    TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA
    ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTCGAAATT TAGTCAATGA TGATGATGCT
    ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG
    ATTGAAACTG ACCATAATGA AGAGGAAGAT GATGAACCTG TTCCTGAGTC CAGTGAGTTA
    ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC
    CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG
    GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT
    AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT
    ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA
    ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA
    CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT
    CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA
    GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT
    GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG
    ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC
    ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC
    TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG
    TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC
    TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT
    GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA
    AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA
    TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC
    CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA
    AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA
    TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA
    GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT
    TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT
    TATGCTAAGG GGTTTGGTAT GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812141.1
    CDS185..2911
    Translation

    Target ORF information:

    RefSeq Version XM_012956687.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956687.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
    AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA
    GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT
    CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT
    GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG
    GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA
    ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA
    AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC
    CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG
    CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC
    CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT
    AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT
    GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA
    GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT
    TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG
    GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT
    ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG
    TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT
    TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA
    ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTCGAAATT TAGTCAATGA TGATGATGCT
    ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG
    ATTGAAACTG ACCATAATGA AGAGGAAGAT GATGAACCTG TTCCTGAGTC CAGTGAGTTA
    ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC
    CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG
    GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT
    AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT
    ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA
    ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA
    CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT
    CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA
    GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT
    GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG
    ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC
    ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC
    TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG
    TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC
    TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT
    GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA
    AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA
    TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC
    CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA
    AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA
    TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA
    GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT
    TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT
    TATGCTAAGG GGTTTGGTAT GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.