ube3a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube3a
Entrez Gene ID	407881
Full Name	ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome)
General protein information	Preferred Names ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) Names ubiquitin-protein ligase E3A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012956687.3	XP_012812141.1	ubiquitin-protein ligase E3A isoform X1
XM_018091154.1	XP_017946643.1	ubiquitin-protein ligase E3A isoform X2
XM_012956687.2	XP_012812141.1	ubiquitin-protein ligase E3A isoform X1
NM_001001213.1	NP_001001213.1	ubiquitin-protein ligase E3A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ube3a	NP_035798.2
Rattus norvegicus (Norway rat)	Ube3a	NP_001178766.1
Danio rerio (zebrafish)	ube3a	NP_001007319.1
Pan troglodytes (chimpanzee)	UBE3A	XP_001161311.1
Bos taurus (cattle)	UBE3A	NP_001091932.1
Gallus gallus (chicken)	UBE3A	XP_004938531.1
Drosophila melanogaster (fruit fly)	Ube3a	NP_648452.1
Homo sapiens (human)	UBE3A	NP_570854.1
	UBE3A	XP_001108661.1
Canis lupus familiaris (dog)	UBE3A	XP_005618288.1
Xenopus tropicalis (tropical clawed frog)	ube3a	NP_001001213.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012366	XP_551819.3

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ube3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24653	NM_001001213.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa26250	XM_012956687.2 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa42494	XM_018091154.1 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa51257	XM_012956687.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube3a ( NM_001001213.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001001213.1 Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), mRNA.

Price & Availability↑

CloneID	OXa24653
Clone ID Related Accession (Same CDS sequence)	NM_001001213.1
Accession Version	NM_001001213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067999.1. ##Evidence-Data-START## Transcript exon combination :: BC067999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGT 
TGTGGAAATG AAACCTGCAC GAATGAATTT TGTGCTTCCT GTCCCACTTT TCTTCGTATG 
GATAACAATG CAGCAGCCAT TAAGGCCCTC GAGTTATATA AGGTTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAACAAGC TCATCTTATC TAGAGAACAA TTCGAAAAGT 
GCCCATAACA ACTCCTGCAC AGAAAGAAAG ATGAATAAGA AAGACCTTCA TGGACCCAGA 
GATGACTTTA AAGATGTCAC TTTCCTGTCT GAAGAAAAAG TGTATGAAAT TCTTGAATTG 
TGTAGAGAAA AAGAGGACTA CTCGCCTTTA ATTCGGGTGA TCGGGAGAGT ATTTTCCAGT 
GCAGAAGCTT TGGTTCAGAG TTTCCGAAAA GCAAAGCACC ATACAAAGGA GGAGCTAAAA 
TCCTTGCAAG AAAAGGATGA AGATAAGGAT GAAGATGAAA AGGAAAAAGC TGCATCATCT 
GCTGCTGCAA TGGAAGAGGA TTCTGGAGCA TCATCCTCAA GGTTAAGTGA CAACTCACAA 
GGAGACAACA ACATACAGAA ACTAGGCCCT GAAGAAGTGT CTCTAGATAT AGAAGCAGTT 
CGACGGGTGT ATCACAGGTT ACTTTCTAAT GAGAAAATAG AAACTGCCTT TCTGAATGCA 
CTTGTGTACC TTTCTCCTAA TGTGGAATGT GACTTAACTT ATCATAATGT TTATTCACGA 
GATCCCAACT ATCTCAATTT GTTTATAATC GTCATGGAGA ATGGTAATCT CCATAGTCCT 
GAATACCTGG AAATGGCTTT ACCGTTGTTT TGCAAAGCAA TGAGTAAATT GCCATTAGCA 
GCGCAAGCAA AGTTGATCCG TCTTTGGTCA AAGTACAGCG CTGAGCAGAT TCGCCGAATG 
ATGGAAACAT TTCAGCAGCT TATAACCTAT AAAGTTATAA GTAACGAGTT CAACAGTCGA 
AATTTAGTCA ATGATGATGA TGCTATTGTT GCAGCAACAA AGTGCTTAAA AATGGTTTAC 
TATTCAAATG TTGTTGGTGG AGAGATTGAA ACTGACCATA ATGAAGAGGA AGATGATGAA 
CCTGTTCCTG AGTCCAGTGA GTTAACTCTG CAGGAGCTGC TAGGGGAAGA ACGAAGAAAC 
AAAAAAGGCC CTCGAGTAGA TCCCCTTGAA ACCGAACTTG GTGTTAAAAG CATTGATTGC 
AGGAAACCTC TCATCCCTTT CGAGGAATTC GTTAACGAAC CACTAAATGA TGTTCTAGAA 
ATGGACAAAG ATTATACTTT CTTTAAAGTA GAGACTGAAA ACAAATTCTC TTTTATGACT 
TGCCCCTTTA TCCTGAATGC AGTTACAAAG AATCTTGGTC TGTATTATGA CAACAGAATC 
CGCATGTACA GTGAAAGGCG AATAACTGTT CTGTATAGTC TAGTGCAAGG ACAGCAACTT 
AACCCCTATT TGAGGCTCAA AGTACGGCGA GATCACATCA TTGACGATGC TCTTGTTAGG 
CTTGAGATGA TTGCCATGGA AAATCCTGCA GATTTGAAGA AGCAATTATA TGTGGAATTT 
GAGGGAGAAC AAGGAGTTGA CGAAGGAGGT GTTTCTAAAG AGTTTTTCCA ATTAGTTGTT 
GAAGAAATTT TCAACCCTGA TATTGGTATG TTTACGTATG ATGAGTCCAC TAAACACTCT 
TGGTTCAATC CATCATCTTT TGAGACAGAA GGACAGTTTA CACTTATTGG CATTGTGCTG 
GGCCTTGCAA TTTACAATAA TTGCATTCTA GATGTGCATT TTCCAATGGT TGTATATAGA 
AAACTAATGG GGAAAAAAGG AACCTTCCGT GACCTTGCTG ATTCTCATCC TGTTCTTTAT 
CAGAGTTTAA AAGAATTGTT GGAGTATGAA GGCAATGTTG AAGAAGATAT GATGATGACT 
TTCCAGATCT CTCAGACTGA CCTCTTTGGA AATCCTTTAA TGCACGACTT AAAGGAAAAT 
GGAGATAAAA TCCCCATTAC AAATGAGAAC AGAAAGGAAT TTGTCAGTCT ATACACAGAC 
TATATTCTAA ATAAATCAGT GGAAAAGCAA TTTAAAGCCT TCAGAAGGGG CTTTCACATG 
GTTACCAATG AATCGCCTCT GAAATATCTG TTTAGACCAG AGGAGATTGA ACTTCTTATT 
TGTGGAAGTA GGAATTTAGA TTTCCAAGCT CTGAAAGATA CTACGGAATA TGATGGTGGC 
TATACCAGAG ATTCAAATAT AATAAAGGAG TTTTGGGAAA TTGTAAATTC TTTCACAGAG 
GAACAAAAAA GATTGTTCTT GCAATTTACA ACAGGCACGG ACAGAGCACC TGTAGGTGGG 
CTAGGCAAAT TAAAGATGAT TATAGCCAAA AACGGCCCTG ATACAGACAG GTTACCAACA 
TCTCATACTT GCTTTAATGT CCTTTTGCTT CCGGAGTACT CAAATAAAGA GAAACTGAAG 
GAGAGATTGT TAAAGGCCAT CACTTATGCT AAGGGGTTTG GTATGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001213.1
CDS	334..2904
Translation	MKRAAAKHLIERYYHQLTEGCGNETCTNEFCASCPTFLRMDNNAAAIKALELYKVNAKLCDPHPSKKGTSSSYLENNSKSAHNNSCTERKMNKKDLHGPRDDFKDVTFLSEEKVYEILELCREKEDYSPLIRVIGRVFSSAEALVQSFRKAKHHTKEELKSLQEKDEDKDEDEKEKAASSAAAMEEDSGASSSRLSDNSQGDNNIQKLGPEEVSLDIEAVRRVYHRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENGNLHSPEYLEMALPLFCKAMSKLPLAAQAKLIRLWSKYSAEQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAATKCLKMVYYSNVVGGEIETDHNEEEDDEPVPESSELTLQELLGEERRNKKGPRVDPLETELGVKSIDCRKPLIPFEEFVNEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKHSWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLADSHPVLYQSLKELLEYEGNVEEDMMMTFQISQTDLFGNPLMHDLKENGDKIPITNENRKEFVSLYTDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALKDTTEYDGGYTRDSNIIKEFWEIVNSFTEEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTDRLPTSHTCFNVLLLPEYSNKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	NM_001001213.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001213.1

ORF Insert Sequence:

ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGT 
TGTGGAAATG AAACCTGCAC GAATGAATTT TGTGCTTCCT GTCCCACTTT TCTTCGTATG 
GATAACAATG CAGCAGCCAT TAAGGCCCTC GAGTTATATA AGGTTAATGC AAAACTCTGT 
GATCCTCATC CCTCCAAGAA AGGAACAAGC TCATCTTATC TAGAGAACAA TTCGAAAAGT 
GCCCATAACA ACTCCTGCAC AGAAAGAAAG ATGAATAAGA AAGACCTTCA TGGACCCAGA 
GATGACTTTA AAGATGTCAC TTTCCTGTCT GAAGAAAAAG TGTATGAAAT TCTTGAATTG 
TGTAGAGAAA AAGAGGACTA CTCGCCTTTA ATTCGGGTGA TCGGGAGAGT ATTTTCCAGT 
GCAGAAGCTT TGGTTCAGAG TTTCCGAAAA GCAAAGCACC ATACAAAGGA GGAGCTAAAA 
TCCTTGCAAG AAAAGGATGA AGATAAGGAT GAAGATGAAA AGGAAAAAGC TGCATCATCT 
GCTGCTGCAA TGGAAGAGGA TTCTGGAGCA TCATCCTCAA GGTTAAGTGA CAACTCACAA 
GGAGACAACA ACATACAGAA ACTAGGCCCT GAAGAAGTGT CTCTAGATAT AGAAGCAGTT 
CGACGGGTGT ATCACAGGTT ACTTTCTAAT GAGAAAATAG AAACTGCCTT TCTGAATGCA 
CTTGTGTACC TTTCTCCTAA TGTGGAATGT GACTTAACTT ATCATAATGT TTATTCACGA 
GATCCCAACT ATCTCAATTT GTTTATAATC GTCATGGAGA ATGGTAATCT CCATAGTCCT 
GAATACCTGG AAATGGCTTT ACCGTTGTTT TGCAAAGCAA TGAGTAAATT GCCATTAGCA 
GCGCAAGCAA AGTTGATCCG TCTTTGGTCA AAGTACAGCG CTGAGCAGAT TCGCCGAATG 
ATGGAAACAT TTCAGCAGCT TATAACCTAT AAAGTTATAA GTAACGAGTT CAACAGTCGA 
AATTTAGTCA ATGATGATGA TGCTATTGTT GCAGCAACAA AGTGCTTAAA AATGGTTTAC 
TATTCAAATG TTGTTGGTGG AGAGATTGAA ACTGACCATA ATGAAGAGGA AGATGATGAA 
CCTGTTCCTG AGTCCAGTGA GTTAACTCTG CAGGAGCTGC TAGGGGAAGA ACGAAGAAAC 
AAAAAAGGCC CTCGAGTAGA TCCCCTTGAA ACCGAACTTG GTGTTAAAAG CATTGATTGC 
AGGAAACCTC TCATCCCTTT CGAGGAATTC GTTAACGAAC CACTAAATGA TGTTCTAGAA 
ATGGACAAAG ATTATACTTT CTTTAAAGTA GAGACTGAAA ACAAATTCTC TTTTATGACT 
TGCCCCTTTA TCCTGAATGC AGTTACAAAG AATCTTGGTC TGTATTATGA CAACAGAATC 
CGCATGTACA GTGAAAGGCG AATAACTGTT CTGTATAGTC TAGTGCAAGG ACAGCAACTT 
AACCCCTATT TGAGGCTCAA AGTACGGCGA GATCACATCA TTGACGATGC TCTTGTTAGG 
CTTGAGATGA TTGCCATGGA AAATCCTGCA GATTTGAAGA AGCAATTATA TGTGGAATTT 
GAGGGAGAAC AAGGAGTTGA CGAAGGAGGT GTTTCTAAAG AGTTTTTCCA ATTAGTTGTT 
GAAGAAATTT TCAACCCTGA TATTGGTATG TTTACGTATG ATGAGTCCAC TAAACACTCT 
TGGTTCAATC CATCATCTTT TGAGACAGAA GGACAGTTTA CACTTATTGG CATTGTGCTG 
GGCCTTGCAA TTTACAATAA TTGCATTCTA GATGTGCATT TTCCAATGGT TGTATATAGA 
AAACTAATGG GGAAAAAAGG AACCTTCCGT GACCTTGCTG ATTCTCATCC TGTTCTTTAT 
CAGAGTTTAA AAGAATTGTT GGAGTATGAA GGCAATGTTG AAGAAGATAT GATGATGACT 
TTCCAGATCT CTCAGACTGA CCTCTTTGGA AATCCTTTAA TGCACGACTT AAAGGAAAAT 
GGAGATAAAA TCCCCATTAC AAATGAGAAC AGAAAGGAAT TTGTCAGTCT ATACACAGAC 
TATATTCTAA ATAAATCAGT GGAAAAGCAA TTTAAAGCCT TCAGAAGGGG CTTTCACATG 
GTTACCAATG AATCGCCTCT GAAATATCTG TTTAGACCAG AGGAGATTGA ACTTCTTATT 
TGTGGAAGTA GGAATTTAGA TTTCCAAGCT CTGAAAGATA CTACGGAATA TGATGGTGGC 
TATACCAGAG ATTCAAATAT AATAAAGGAG TTTTGGGAAA TTGTAAATTC TTTCACAGAG 
GAACAAAAAA GATTGTTCTT GCAATTTACA ACAGGCACGG ACAGAGCACC TGTAGGTGGG 
CTAGGCAAAT TAAAGATGAT TATAGCCAAA AACGGCCCTG ATACAGACAG GTTACCAACA 
TCTCATACTT GCTTTAATGT CCTTTTGCTT CCGGAGTACT CAAATAAAGA GAAACTGAAG 
GAGAGATTGT TAAAGGCCAT CACTTATGCT AAGGGGTTTG GTATGCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3a ( XM_012956687.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812141.1 Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26250
Clone ID Related Accession (Same CDS sequence)	XM_012956687.2
Accession Version	XM_012956687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA 
GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT 
CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT 
GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG 
GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA 
ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA 
AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC 
CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG 
CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC 
CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT 
GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA 
GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT 
TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG 
GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT 
ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG 
TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT 
TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA 
ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTAGAAATT TAGTCAATGA TGATGATGCT 
ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG 
ATTGAAACTG ACCATAATGA AGAGGAAGAC GATGAACCTG TTCCTGAGTC CAGTGAGTTA 
ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC 
CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG 
GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT 
AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT 
ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA 
ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA 
CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT 
CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA 
GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT 
GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG 
ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC 
ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC 
TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG 
TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC 
TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT 
GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA 
AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA 
TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC 
CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA 
AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA 
TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA 
GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT 
TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT 
TATGCTAAGG GGTTTGGTAT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812141.1
CDS	194..2920
Translation	MSSPDKRWQQEREGAPEPEERRTNTPRSSPGHSPEGDRSPGDPYSENLENSRMKRAAAKHLIERYYHQLTEGCGNETCTNEFCASCPTFLRMDNNAAAIKALELYKVNAKLCDPHPSKKGTSSSYLENNSKSAHNNSCTERKMNKKDLHGPRDDFKDVTFLSEEKVYEILELCREKEDYSPLIRVIGRVFSSAEALVQSFRKAKHHTKEELKSLQEKDEDKDEDEKEKAASSAAAMEEDSGASSSRLSDNSQGDNNIQKLGPEEVSLDIEAVRRVYHRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENGNLHSPEYLEMALPLFCKAMSKLPLAAQAKLIRLWSKYSAEQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAATKCLKMVYYSNVVGGEIETDHNEEEDDEPVPESSELTLQELLGEERRNKKGPRVDPLETELGVKSIDCRKPLIPFEEFVNEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKHSWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLADSHPVLYQSLKELLEYEGNVEEDMMMTFQISQTDLFGNPLMHDLKENGDKIPITNENRKEFVSLYTDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALKDTTEYDGGYTRDSNIIKEFWEIVNSFTEEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTDRLPTSHTCFNVLLLPEYSNKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_012956687.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956687.2

ORF Insert Sequence:

ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA 
GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT 
CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT 
GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG 
GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA 
ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA 
AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC 
CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG 
CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC 
CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT 
GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA 
GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT 
TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG 
GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT 
ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG 
TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT 
TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA 
ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTAGAAATT TAGTCAATGA TGATGATGCT 
ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG 
ATTGAAACTG ACCATAATGA AGAGGAAGAC GATGAACCTG TTCCTGAGTC CAGTGAGTTA 
ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC 
CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG 
GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT 
AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT 
ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA 
ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA 
CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT 
CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA 
GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT 
GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG 
ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC 
ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC 
TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG 
TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC 
TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT 
GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA 
AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA 
TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC 
CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA 
AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA 
TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA 
GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT 
TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT 
TATGCTAAGG GGTTTGGTAT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3a ( XM_018091154.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946643.1 Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42494
Clone ID Related Accession (Same CDS sequence)	XM_018091154.1
Accession Version	XM_018091154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGGAAGCGA 
GCAGCTGCAA AGCATCTAAT AGAACGCTAC TACCACCAGT TAACTGAGGG TTGTGGAAAT 
GAAACCTGCA CGAATGAATT TTGTGCTTCC TGTCCCACTT TTCTTCGTAT GGATAACAAT 
GCAGCAGCCA TTAAGGCCCT CGAGTTATAT AAGGTTAATG CAAAACTCTG TGATCCTCAT 
CCCTCCAAGA AAGGAACAAG CTCATCTTAT CTAGAGAACA ATTCGAAAAG TGCCCATAAC 
AACTCCTGCA CAGAAAGAAA GATGAATAAG AAAGACCTTC ATGGACCCAG AGATGACTTT 
AAAGATGTCA CTTTCCTGTC TGAAGAAAAA GTGTATGAAA TTCTTGAATT GTGTAGAGAA 
AAAGAGGACT ACTCGCCTTT AATTCGGGTG ATCGGGAGAG TATTTTCCAG TGCAGAAGCT 
TTGGTTCAGA GTTTCCGAAA AGCAAAGCAC CATACAAAGG AGGAGCTAAA ATCCTTGCAA 
GAAAAGGATG AAGATAAGGA TGAAGATGAA AAGGAAAAAG CTGCATCATC TGCTGCTGCA 
ATGGAAGAGG ATTCTGGAGC ATCATCCTCA AGGTTAAGTG ACAACTCACA AGGAGACAAC 
AACATACAGA AACTAGGCCC TGAAGAAGTG TCTCTAGATA TAGAAGCAGT TCGACGGGTG 
TATCACAGGT TACTTTCTAA TGAGAAAATA GAAACTGCCT TTCTGAATGC ACTTGTGTAC 
CTTTCTCCTA ATGTGGAATG TGACTTAACT TATCATAATG TTTATTCACG AGATCCCAAC 
TATCTCAATT TGTTTATAAT CGTCATGGAG AATGGTAATC TCCATAGTCC TGAATACCTG 
GAAATGGCTT TACCGTTGTT TTGCAAAGCA ATGAGTAAAT TGCCATTAGC AGCGCAAGCA 
AAGTTGATCC GTCTTTGGTC AAAGTACAGC GCTGAGCAGA TTCGCCGAAT GATGGAAACA 
TTTCAGCAGC TTATAACCTA TAAAGTTATA AGTAACGAGT TCAACAGTAG AAATTTAGTC 
AATGATGATG ATGCTATTGT TGCAGCAACA AAGTGCTTAA AAATGGTTTA CTATTCAAAT 
GTTGTTGGTG GAGAGATTGA AACTGACCAT AATGAAGAGG AAGACGATGA ACCTGTTCCT 
GAGTCCAGTG AGTTAACTCT GCAGGAGCTG CTAGGGGAAG AACGAAGAAA CAAAAAAGGC 
CCTCGAGTAG ATCCCCTTGA AACCGAACTT GGTGTTAAAA GCATTGATTG CAGGAAACCT 
CTCATCCCTT TCGAGGAATT CGTTAACGAA CCACTAAATG ATGTTCTAGA AATGGACAAA 
GATTATACTT TCTTTAAAGT AGAGACTGAA AACAAATTCT CTTTTATGAC TTGCCCCTTT 
ATCCTGAATG CAGTTACAAA GAATCTTGGT CTGTATTATG ACAACAGAAT CCGCATGTAC 
AGTGAAAGGC GAATAACTGT TCTGTATAGT CTAGTGCAAG GACAGCAACT TAACCCCTAT 
TTGAGGCTCA AAGTACGGCG AGATCACATC ATTGACGATG CTCTTGTTAG GCTTGAGATG 
ATTGCCATGG AAAATCCTGC AGATTTGAAG AAGCAATTAT ATGTGGAATT TGAGGGAGAA 
CAAGGAGTTG ACGAAGGAGG TGTTTCTAAA GAGTTTTTCC AATTAGTTGT TGAAGAAATT 
TTCAACCCTG ATATTGGTAT GTTTACGTAT GATGAGTCCA CTAAACACTC TTGGTTCAAT 
CCATCATCTT TTGAGACAGA AGGACAGTTT ACACTTATTG GCATTGTGCT GGGCCTTGCA 
ATTTACAATA ATTGCATTCT AGATGTGCAT TTTCCAATGG TTGTATATAG AAAACTAATG 
GGGAAAAAAG GAACCTTCCG TGACCTTGCT GATTCTCATC CTGTTCTTTA TCAGAGTTTA 
AAAGAATTGT TGGAGTATGA AGGCAATGTT GAAGAAGATA TGATGATGAC TTTCCAGATC 
TCTCAGACTG ACCTCTTTGG AAATCCTTTA ATGCACGACT TAAAGGAAAA TGGAGATAAA 
ATCCCCATTA CAAATGAGAA CAGAAAGGAA TTTGTCAGTC TATACACAGA CTATATTCTA 
AATAAATCAG TGGAAAAGCA ATTTAAAGCC TTCAGAAGGG GCTTTCACAT GGTTACCAAT 
GAATCGCCTC TGAAATATCT GTTTAGACCA GAGGAGATTG AACTTCTTAT TTGTGGAAGT 
AGGAATTTAG ATTTCCAAGC TCTGAAAGAT ACTACGGAAT ATGATGGTGG CTATACCAGA 
GATTCAAATA TAATAAAGGA GTTTTGGGAA ATTGTAAATT CTTTCACAGA GGAACAAAAA 
AGATTGTTCT TGCAATTTAC AACAGGCACG GACAGAGCAC CTGTAGGTGG GCTAGGCAAA 
TTAAAGATGA TTATAGCCAA AAACGGCCCT GATACAGACA GGTTACCAAC ATCTCATACT 
TGCTTTAATG TCCTTTTGCT TCCGGAGTAC TCAAATAAAG AGAAACTGAA GGAGAGATTG 
TTAAAGGCCA TCACTTATGC TAAGGGGTTT GGTATGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946643.1
CDS	195..2876
Translation	MSSPDKRWQQEREGAPEPEERRTNTPRSSPGHSPEGDRKRAAAKHLIERYYHQLTEGCGNETCTNEFCASCPTFLRMDNNAAAIKALELYKVNAKLCDPHPSKKGTSSSYLENNSKSAHNNSCTERKMNKKDLHGPRDDFKDVTFLSEEKVYEILELCREKEDYSPLIRVIGRVFSSAEALVQSFRKAKHHTKEELKSLQEKDEDKDEDEKEKAASSAAAMEEDSGASSSRLSDNSQGDNNIQKLGPEEVSLDIEAVRRVYHRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENGNLHSPEYLEMALPLFCKAMSKLPLAAQAKLIRLWSKYSAEQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAATKCLKMVYYSNVVGGEIETDHNEEEDDEPVPESSELTLQELLGEERRNKKGPRVDPLETELGVKSIDCRKPLIPFEEFVNEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKHSWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLADSHPVLYQSLKELLEYEGNVEEDMMMTFQISQTDLFGNPLMHDLKENGDKIPITNENRKEFVSLYTDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALKDTTEYDGGYTRDSNIIKEFWEIVNSFTEEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTDRLPTSHTCFNVLLLPEYSNKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_018091154.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091154.1

ORF Insert Sequence:

ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGGAAGCGA 
GCAGCTGCAA AGCATCTAAT AGAACGCTAC TACCACCAGT TAACTGAGGG TTGTGGAAAT 
GAAACCTGCA CGAATGAATT TTGTGCTTCC TGTCCCACTT TTCTTCGTAT GGATAACAAT 
GCAGCAGCCA TTAAGGCCCT CGAGTTATAT AAGGTTAATG CAAAACTCTG TGATCCTCAT 
CCCTCCAAGA AAGGAACAAG CTCATCTTAT CTAGAGAACA ATTCGAAAAG TGCCCATAAC 
AACTCCTGCA CAGAAAGAAA GATGAATAAG AAAGACCTTC ATGGACCCAG AGATGACTTT 
AAAGATGTCA CTTTCCTGTC TGAAGAAAAA GTGTATGAAA TTCTTGAATT GTGTAGAGAA 
AAAGAGGACT ACTCGCCTTT AATTCGGGTG ATCGGGAGAG TATTTTCCAG TGCAGAAGCT 
TTGGTTCAGA GTTTCCGAAA AGCAAAGCAC CATACAAAGG AGGAGCTAAA ATCCTTGCAA 
GAAAAGGATG AAGATAAGGA TGAAGATGAA AAGGAAAAAG CTGCATCATC TGCTGCTGCA 
ATGGAAGAGG ATTCTGGAGC ATCATCCTCA AGGTTAAGTG ACAACTCACA AGGAGACAAC 
AACATACAGA AACTAGGCCC TGAAGAAGTG TCTCTAGATA TAGAAGCAGT TCGACGGGTG 
TATCACAGGT TACTTTCTAA TGAGAAAATA GAAACTGCCT TTCTGAATGC ACTTGTGTAC 
CTTTCTCCTA ATGTGGAATG TGACTTAACT TATCATAATG TTTATTCACG AGATCCCAAC 
TATCTCAATT TGTTTATAAT CGTCATGGAG AATGGTAATC TCCATAGTCC TGAATACCTG 
GAAATGGCTT TACCGTTGTT TTGCAAAGCA ATGAGTAAAT TGCCATTAGC AGCGCAAGCA 
AAGTTGATCC GTCTTTGGTC AAAGTACAGC GCTGAGCAGA TTCGCCGAAT GATGGAAACA 
TTTCAGCAGC TTATAACCTA TAAAGTTATA AGTAACGAGT TCAACAGTAG AAATTTAGTC 
AATGATGATG ATGCTATTGT TGCAGCAACA AAGTGCTTAA AAATGGTTTA CTATTCAAAT 
GTTGTTGGTG GAGAGATTGA AACTGACCAT AATGAAGAGG AAGACGATGA ACCTGTTCCT 
GAGTCCAGTG AGTTAACTCT GCAGGAGCTG CTAGGGGAAG AACGAAGAAA CAAAAAAGGC 
CCTCGAGTAG ATCCCCTTGA AACCGAACTT GGTGTTAAAA GCATTGATTG CAGGAAACCT 
CTCATCCCTT TCGAGGAATT CGTTAACGAA CCACTAAATG ATGTTCTAGA AATGGACAAA 
GATTATACTT TCTTTAAAGT AGAGACTGAA AACAAATTCT CTTTTATGAC TTGCCCCTTT 
ATCCTGAATG CAGTTACAAA GAATCTTGGT CTGTATTATG ACAACAGAAT CCGCATGTAC 
AGTGAAAGGC GAATAACTGT TCTGTATAGT CTAGTGCAAG GACAGCAACT TAACCCCTAT 
TTGAGGCTCA AAGTACGGCG AGATCACATC ATTGACGATG CTCTTGTTAG GCTTGAGATG 
ATTGCCATGG AAAATCCTGC AGATTTGAAG AAGCAATTAT ATGTGGAATT TGAGGGAGAA 
CAAGGAGTTG ACGAAGGAGG TGTTTCTAAA GAGTTTTTCC AATTAGTTGT TGAAGAAATT 
TTCAACCCTG ATATTGGTAT GTTTACGTAT GATGAGTCCA CTAAACACTC TTGGTTCAAT 
CCATCATCTT TTGAGACAGA AGGACAGTTT ACACTTATTG GCATTGTGCT GGGCCTTGCA 
ATTTACAATA ATTGCATTCT AGATGTGCAT TTTCCAATGG TTGTATATAG AAAACTAATG 
GGGAAAAAAG GAACCTTCCG TGACCTTGCT GATTCTCATC CTGTTCTTTA TCAGAGTTTA 
AAAGAATTGT TGGAGTATGA AGGCAATGTT GAAGAAGATA TGATGATGAC TTTCCAGATC 
TCTCAGACTG ACCTCTTTGG AAATCCTTTA ATGCACGACT TAAAGGAAAA TGGAGATAAA 
ATCCCCATTA CAAATGAGAA CAGAAAGGAA TTTGTCAGTC TATACACAGA CTATATTCTA 
AATAAATCAG TGGAAAAGCA ATTTAAAGCC TTCAGAAGGG GCTTTCACAT GGTTACCAAT 
GAATCGCCTC TGAAATATCT GTTTAGACCA GAGGAGATTG AACTTCTTAT TTGTGGAAGT 
AGGAATTTAG ATTTCCAAGC TCTGAAAGAT ACTACGGAAT ATGATGGTGG CTATACCAGA 
GATTCAAATA TAATAAAGGA GTTTTGGGAA ATTGTAAATT CTTTCACAGA GGAACAAAAA 
AGATTGTTCT TGCAATTTAC AACAGGCACG GACAGAGCAC CTGTAGGTGG GCTAGGCAAA 
TTAAAGATGA TTATAGCCAA AAACGGCCCT GATACAGACA GGTTACCAAC ATCTCATACT 
TGCTTTAATG TCCTTTTGCT TCCGGAGTAC TCAAATAAAG AGAAACTGAA GGAGAGATTG 
TTAAAGGCCA TCACTTATGC TAAGGGGTTT GGTATGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3a ( XM_012956687.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812141.1 Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa51257
Clone ID Related Accession (Same CDS sequence)	XM_012956687.3
Accession Version	XM_012956687.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin-protein ligase E3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA 
GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT 
CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT 
GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG 
GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA 
ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA 
AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC 
CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG 
CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC 
CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT 
GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA 
GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT 
TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG 
GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT 
ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG 
TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT 
TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA 
ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTCGAAATT TAGTCAATGA TGATGATGCT 
ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG 
ATTGAAACTG ACCATAATGA AGAGGAAGAT GATGAACCTG TTCCTGAGTC CAGTGAGTTA 
ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC 
CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG 
GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT 
AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT 
ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA 
ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA 
CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT 
CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA 
GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT 
GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG 
ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC 
ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC 
TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG 
TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC 
TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT 
GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA 
AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA 
TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC 
CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA 
AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA 
TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA 
GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT 
TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT 
TATGCTAAGG GGTTTGGTAT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812141.1
CDS	185..2911
Translation	MSSPDKRWQQEREGAPEPEERRTNTPRSSPGHSPEGDRSPGDPYSENLENSRMKRAAAKHLIERYYHQLTEGCGNETCTNEFCASCPTFLRMDNNAAAIKALELYKVNAKLCDPHPSKKGTSSSYLENNSKSAHNNSCTERKMNKKDLHGPRDDFKDVTFLSEEKVYEILELCREKEDYSPLIRVIGRVFSSAEALVQSFRKAKHHTKEELKSLQEKDEDKDEDEKEKAASSAAAMEEDSGASSSRLSDNSQGDNNIQKLGPEEVSLDIEAVRRVYHRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENGNLHSPEYLEMALPLFCKAMSKLPLAAQAKLIRLWSKYSAEQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAATKCLKMVYYSNVVGGEIETDHNEEEDDEPVPESSELTLQELLGEERRNKKGPRVDPLETELGVKSIDCRKPLIPFEEFVNEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKHSWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLADSHPVLYQSLKELLEYEGNVEEDMMMTFQISQTDLFGNPLMHDLKENGDKIPITNENRKEFVSLYTDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALKDTTEYDGGYTRDSNIIKEFWEIVNSFTEEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTDRLPTSHTCFNVLLLPEYSNKEKLKERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_012956687.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) (ube3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956687.3

ORF Insert Sequence:

ATGAGTTCCC CGGACAAGAG GTGGCAGCAG GAGAGAGAGG GAGCCCCGGA GCCCGAGGAG 
AGGAGGACCA ACACCCCCCG CAGCAGCCCG GGACACAGCC CTGAAGGAGA CAGATCACCA 
GGAGACCCTT ATTCAGAAAA TCTAGAAAAT AGCCGAATGA AGCGAGCAGC TGCAAAGCAT 
CTAATAGAAC GCTACTACCA CCAGTTAACT GAGGGTTGTG GAAATGAAAC CTGCACGAAT 
GAATTTTGTG CTTCCTGTCC CACTTTTCTT CGTATGGATA ACAATGCAGC AGCCATTAAG 
GCCCTCGAGT TATATAAGGT TAATGCAAAA CTCTGTGATC CTCATCCCTC CAAGAAAGGA 
ACAAGCTCAT CTTATCTAGA GAACAATTCG AAAAGTGCCC ATAACAACTC CTGCACAGAA 
AGAAAGATGA ATAAGAAAGA CCTTCATGGA CCCAGAGATG ACTTTAAAGA TGTCACTTTC 
CTGTCTGAAG AAAAAGTGTA TGAAATTCTT GAATTGTGTA GAGAAAAAGA GGACTACTCG 
CCTTTAATTC GGGTGATCGG GAGAGTATTT TCCAGTGCAG AAGCTTTGGT TCAGAGTTTC 
CGAAAAGCAA AGCACCATAC AAAGGAGGAG CTAAAATCCT TGCAAGAAAA GGATGAAGAT 
AAGGATGAAG ATGAAAAGGA AAAAGCTGCA TCATCTGCTG CTGCAATGGA AGAGGATTCT 
GGAGCATCAT CCTCAAGGTT AAGTGACAAC TCACAAGGAG ACAACAACAT ACAGAAACTA 
GGCCCTGAAG AAGTGTCTCT AGATATAGAA GCAGTTCGAC GGGTGTATCA CAGGTTACTT 
TCTAATGAGA AAATAGAAAC TGCCTTTCTG AATGCACTTG TGTACCTTTC TCCTAATGTG 
GAATGTGACT TAACTTATCA TAATGTTTAT TCACGAGATC CCAACTATCT CAATTTGTTT 
ATAATCGTCA TGGAGAATGG TAATCTCCAT AGTCCTGAAT ACCTGGAAAT GGCTTTACCG 
TTGTTTTGCA AAGCAATGAG TAAATTGCCA TTAGCAGCGC AAGCAAAGTT GATCCGTCTT 
TGGTCAAAGT ACAGCGCTGA GCAGATTCGC CGAATGATGG AAACATTTCA GCAGCTTATA 
ACCTATAAAG TTATAAGTAA CGAGTTCAAC AGTCGAAATT TAGTCAATGA TGATGATGCT 
ATTGTTGCAG CAACAAAGTG CTTAAAAATG GTTTACTATT CAAATGTTGT TGGTGGAGAG 
ATTGAAACTG ACCATAATGA AGAGGAAGAT GATGAACCTG TTCCTGAGTC CAGTGAGTTA 
ACTCTGCAGG AGCTGCTAGG GGAAGAACGA AGAAACAAAA AAGGCCCTCG AGTAGATCCC 
CTTGAAACCG AACTTGGTGT TAAAAGCATT GATTGCAGGA AACCTCTCAT CCCTTTCGAG 
GAATTCGTTA ACGAACCACT AAATGATGTT CTAGAAATGG ACAAAGATTA TACTTTCTTT 
AAAGTAGAGA CTGAAAACAA ATTCTCTTTT ATGACTTGCC CCTTTATCCT GAATGCAGTT 
ACAAAGAATC TTGGTCTGTA TTATGACAAC AGAATCCGCA TGTACAGTGA AAGGCGAATA 
ACTGTTCTGT ATAGTCTAGT GCAAGGACAG CAACTTAACC CCTATTTGAG GCTCAAAGTA 
CGGCGAGATC ACATCATTGA CGATGCTCTT GTTAGGCTTG AGATGATTGC CATGGAAAAT 
CCTGCAGATT TGAAGAAGCA ATTATATGTG GAATTTGAGG GAGAACAAGG AGTTGACGAA 
GGAGGTGTTT CTAAAGAGTT TTTCCAATTA GTTGTTGAAG AAATTTTCAA CCCTGATATT 
GGTATGTTTA CGTATGATGA GTCCACTAAA CACTCTTGGT TCAATCCATC ATCTTTTGAG 
ACAGAAGGAC AGTTTACACT TATTGGCATT GTGCTGGGCC TTGCAATTTA CAATAATTGC 
ATTCTAGATG TGCATTTTCC AATGGTTGTA TATAGAAAAC TAATGGGGAA AAAAGGAACC 
TTCCGTGACC TTGCTGATTC TCATCCTGTT CTTTATCAGA GTTTAAAAGA ATTGTTGGAG 
TATGAAGGCA ATGTTGAAGA AGATATGATG ATGACTTTCC AGATCTCTCA GACTGACCTC 
TTTGGAAATC CTTTAATGCA CGACTTAAAG GAAAATGGAG ATAAAATCCC CATTACAAAT 
GAGAACAGAA AGGAATTTGT CAGTCTATAC ACAGACTATA TTCTAAATAA ATCAGTGGAA 
AAGCAATTTA AAGCCTTCAG AAGGGGCTTT CACATGGTTA CCAATGAATC GCCTCTGAAA 
TATCTGTTTA GACCAGAGGA GATTGAACTT CTTATTTGTG GAAGTAGGAA TTTAGATTTC 
CAAGCTCTGA AAGATACTAC GGAATATGAT GGTGGCTATA CCAGAGATTC AAATATAATA 
AAGGAGTTTT GGGAAATTGT AAATTCTTTC ACAGAGGAAC AAAAAAGATT GTTCTTGCAA 
TTTACAACAG GCACGGACAG AGCACCTGTA GGTGGGCTAG GCAAATTAAA GATGATTATA 
GCCAAAAACG GCCCTGATAC AGACAGGTTA CCAACATCTC ATACTTGCTT TAATGTCCTT 
TTGCTTCCGG AGTACTCAAA TAAAGAGAAA CTGAAGGAGA GATTGTTAAA GGCCATCACT 
TATGCTAAGG GGTTTGGTAT GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ube3a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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