UBE3A cDNA ORF clone, Canis lupus familiaris(dog)

The following UBE3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe04153 XM_014112297.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04153 XM_005618230.3
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04153 XM_014112298.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04153 XM_014112295.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04153 XM_005618231.2
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04153 XM_022416813.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04153 XM_014112296.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04154 XM_022416814.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04154 XM_014112299.2
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04155 XM_005618232.3
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04155 XM_005618234.3
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04156 XM_022416816.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe04156 XM_014112300.2
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe287171 XM_022416815.1
Latest version!
Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe04155 XM_005618234.2 Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04154 XM_014112299.1 Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04156 XM_014112300.1 Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe04153 XM_005618230.2 Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe04155 XM_005618232.2 Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_005618230.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618287.1
    CDS321..3080
    Translation

    Target ORF information:

    RefSeq Version XM_005618230.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618230.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_005618231.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618288.1
    CDS888..3647
    Translation

    Target ORF information:

    RefSeq Version XM_005618231.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618231.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_014112295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967770.1
    CDS837..3596
    Translation

    Target ORF information:

    RefSeq Version XM_014112295.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_014112298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967773.1
    CDS217..2976
    Translation

    Target ORF information:

    RefSeq Version XM_014112298.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_022416813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272521.1
    CDS372..3131
    Translation

    Target ORF information:

    RefSeq Version XM_022416813.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_014112297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967772.1
    CDS337..3096
    Translation

    Target ORF information:

    RefSeq Version XM_014112297.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_005618230.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618287.1
    CDS715..3474
    Translation

    Target ORF information:

    RefSeq Version XM_005618230.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04153
    Clone ID Related Accession (Same CDS sequence) XM_005618230.3 , XM_005618231.2 , XM_014112295.1 , XM_014112298.1 , XM_022416813.1 , XM_014112297.1 , XM_005618230.2 , XM_014112296.1
    Accession Version XM_014112296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967771.1
    CDS858..3617
    Translation

    Target ORF information:

    RefSeq Version XM_014112296.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GTCCTCTATC AGAGTTTAAA AGATTTATTG GAATATGAAG GGAATGTGGA AGATGACATG
    ATGATCACTT TCCAGATATC TCAGACAGAC CTCTTTGGTA ATCCCATGAT GTATGATTTA
    AAGGAAAATG GTGATAAAAT TCCAATTACA AATGAAAATA GGAAGGAATT TGTCAATCTC
    TATTCTGACT ACATTCTCAA TAAATCAGTA GAAAAACAGT TCAAAGCTTT TCGGAGAGGT
    TTTCATATGG TGACCAACGA ATCTCCTTTA AAGTATTTAT TCAGACCAGA GGAAATTGAA
    TTGCTTATAT GTGGAAGCCG GAATCTAGAT TTCCAAGCAC TAGAAGAAAC TACAGAATAT
    GACGGTGGCT ATACCAGGGA CTCTGTTCTG ATTAGAGAGT TCTGGGAAAT CGTTCATTCA
    TTTACAGATG AGCAGAAAAG ACTCTTCTTG CAGTTTACAA CAGGCACAGA CAGAGCACCT
    GTGGGCGGAC TAGGAAAATT AAAGATGATT ATAGCCAAAA ATGGCCCAGA CACAGAAAGG
    TTACCTACAT CTCATACTTG CTTTAATGTC CTTTTACTGC CGGAATATTC AAGCAAAGAA
    AAACTTAAAG AAAGATTGTT GAAGGCCATC ACTTACGCCA AAGGATTTGG CATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04154
    Clone ID Related Accession (Same CDS sequence) XM_022416814.1 , XM_014112299.1 , XM_014112299.2
    Accession Version XM_022416814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCGCCAGGA GAACCTCAGT CTGACGACAT CGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCTTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272522.1
    CDS290..2917
    Translation

    Target ORF information:

    RefSeq Version XM_022416814.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCGCCAGGA GAACCTCAGT CTGACGACAT CGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCTTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04154
    Clone ID Related Accession (Same CDS sequence) XM_022416814.1 , XM_014112299.1 , XM_014112299.2
    Accession Version XM_014112299.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCGCCAGGA GAACCTCAGT CTGACGACAT CGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCTTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967774.1
    CDS806..3433
    Translation

    Target ORF information:

    RefSeq Version XM_014112299.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCGCCAGGA GAACCTCAGT CTGACGACAT CGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCTTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04154
    Clone ID Related Accession (Same CDS sequence) XM_022416814.1 , XM_014112299.1 , XM_014112299.2
    Accession Version XM_014112299.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCGCCAGGA GAACCTCAGT CTGACGACAT CGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCTTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967774.1
    CDS412..3039
    Translation

    Target ORF information:

    RefSeq Version XM_014112299.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112299.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCGCCAGGA GAACCTCAGT CTGACGACAT CGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAGCATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCTTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04155
    Clone ID Related Accession (Same CDS sequence) XM_005618234.3 , XM_005618234.2 , XM_005618232.2 , XM_005618232.3
    Accession Version XM_005618234.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618291.1
    CDS123..2687
    Translation

    Target ORF information:

    RefSeq Version XM_005618234.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618234.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04155
    Clone ID Related Accession (Same CDS sequence) XM_005618234.3 , XM_005618234.2 , XM_005618232.2 , XM_005618232.3
    Accession Version XM_005618234.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618291.1
    CDS172..2736
    Translation

    Target ORF information:

    RefSeq Version XM_005618234.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04155
    Clone ID Related Accession (Same CDS sequence) XM_005618234.3 , XM_005618234.2 , XM_005618232.2 , XM_005618232.3
    Accession Version XM_005618232.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618232.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618289.1
    CDS134..2698
    Translation

    Target ORF information:

    RefSeq Version XM_005618232.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04155
    Clone ID Related Accession (Same CDS sequence) XM_005618234.3 , XM_005618234.2 , XM_005618232.2 , XM_005618232.3
    Accession Version XM_005618232.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618289.1
    CDS183..2747
    Translation

    Target ORF information:

    RefSeq Version XM_005618232.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618232.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA GCATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCTTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGT TCAGCTTACC TTGAAAACTC GAAAGGTGCC
    CCTAACAACT CCTGCTCTGA CATAAAAATG AACAAGAAGG AAGGACAAGG AGCAAGAGAT
    GATTTTAGAG ATGTGACTTA CCTAACAGAA GAGACGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATC CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAGGCATTGG TACAGAGCTT TCGGAAAGTC AAACAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA CAAGGATGAA GATGAAAAGG AAAAAGCTGC ATGTTCTGCT
    GCTATGGAAG AAGATTCAGA AGCATCTTCT TCAAGGATAA GTGATAGTTC ACAAGGAGAT
    AACAACTTAC AAAAAATAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC CATTAGAAGG
    GTCTATACCA GATTACTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC TCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTAATG GAGAATAGAA ATCTACACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCATT GTTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAATTGG TTAGACTGTG GTCTAAATAC AGTGCAGACC AAATTCGTAG AATGATGGAA
    ACATTTCAGC AACTTATTAC TTACAAAGTC ATAAGCAATG AATTTAACAG TCGGAATCTA
    GTGAATGATG ATGATGCTAT TGTTGCTGCT TCAAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTGG GTGGGGAAGT GGACACAAAT CACAATGAAG AAGATGATGA AGAGCCCATC
    CCTGAATCCA GTGAATTGAC ACTTCAGGAG CTTTTGGGAG AGGAAAGAAG AAATAAGAAA
    GGTCCTCGAG TGGACCCATT GGAAACTGAA CTTGGTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAAT TCTCTTTCAT GACATGTCCC
    TTTATATTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATCAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAATT ATTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT GCTGGGTCTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCGTGACCTA GGAGACTCTC ACCCTGTCCT CTATCAGAGT
    TTAAAAGATT TATTGGAATA TGAAGGGAAT GTGGAAGATG ACATGATGAT CACTTTCCAG
    ATATCTCAGA CAGACCTCTT TGGTAATCCC ATGATGTATG ATTTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACAAATGA AAATAGGAAG GAATTTGTCA ATCTCTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAA GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AACGAATCTC CTTTAAAGTA TTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTCTGATTAG AGAGTTCTGG GAAATCGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGAG CACCTGTGGG CGGACTAGGA
    AAATTAAAGA TGATTATAGC CAAAAATGGC CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAAAGA
    TTGTTGAAGG CCATCACTTA CGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04156
    Clone ID Related Accession (Same CDS sequence) XM_014112300.2 , XM_014112300.1 , XM_022416816.1
    Accession Version XM_014112300.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967775.1
    CDS235..2682
    Translation

    Target ORF information:

    RefSeq Version XM_014112300.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04156
    Clone ID Related Accession (Same CDS sequence) XM_014112300.2 , XM_014112300.1 , XM_022416816.1
    Accession Version XM_014112300.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ubiquitin-protein ligase E3A isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726053.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967775.1
    CDS623..3070
    Translation

    Target ORF information:

    RefSeq Version XM_014112300.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04156
    Clone ID Related Accession (Same CDS sequence) XM_014112300.2 , XM_014112300.1 , XM_022416816.1
    Accession Version XM_022416816.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272524.1
    CDS134..2581
    Translation

    Target ORF information:

    RefSeq Version XM_022416816.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416816.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGTTCAGCTT ACCTTGAAAA CTCGAAAGGT
    GCCCCTAACA ACTCCTGCTC TGACATAAAA ATGAACAAGA AGGAAGGACA AGGAGCAAGA
    GATGATTTTA GAGATGTGAC TTACCTAACA GAAGAGACGG TGTATGAAAT TCTTGAATTA
    TGTAGAGAGA GAGAGGATTA TTCCCCTTTA ATCCGTGTTA TTGGAAGAGT TTTTTCTAGT
    GCTGAGGCAT TGGTACAGAG CTTTCGGAAA GTCAAACAAC ACACCAAGGA AGAACTGAAA
    TCTCTTCAAG GAAAGGATGA AGACAAGGAT GAAGATGAAA AGGAAAAAGC TGCATGTTCT
    GCTGCTATGG AAGAAGATTC AGAAGCATCT TCTTCAAGGA TAAGTGATAG TTCACAAGGA
    GATAACAACT TACAAAAAAT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCCATTAGA
    AGGGTCTATA CCAGATTACT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCTCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTA ATGGAGAATA GAAATCTACA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ATTGTTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAT TGGTTAGACT GTGGTCTAAA TACAGTGCAG ACCAAATTCG TAGAATGATG
    GAAACATTTC AGCAACTTAT TACTTACAAA GTCATAAGCA ATGAATTTAA CAGTCGGAAT
    CTAGTGAATG ATGATGATGC TATTGTTGCT GCTTCAAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TGGGTGGGGA AGTGGACACA AATCACAATG AAGAAGATGA TGAAGAGCCC
    ATCCCTGAAT CCAGTGAATT GACACTTCAG GAGCTTTTGG GAGAGGAAAG AAGAAATAAG
    AAAGGTCCTC GAGTGGACCC ATTGGAAACT GAACTTGGTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AATTCTCTTT CATGACATGT
    CCCTTTATAT TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT CACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA ATTATTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTGCTGGGT
    CTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCGTGAC CTAGGAGACT CTCACCCTGT CCTCTATCAG
    AGTTTAAAAG ATTTATTGGA ATATGAAGGG AATGTGGAAG ATGACATGAT GATCACTTTC
    CAGATATCTC AGACAGACCT CTTTGGTAAT CCCATGATGT ATGATTTAAA GGAAAATGGT
    GATAAAATTC CAATTACAAA TGAAAATAGG AAGGAATTTG TCAATCTCTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAAGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAACGAAT CTCCTTTAAA GTATTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTCTGAT TAGAGAGTTC TGGGAAATCG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GAGCACCTGT GGGCGGACTA
    GGAAAATTAA AGATGATTAT AGCCAAAAAT GGCCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTGCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAA
    AGATTGTTGA AGGCCATCAC TTACGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287171
    Clone ID Related Accession (Same CDS sequence) XM_022416815.1
    Accession Version XM_022416815.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ubiquitin-protein ligase E3A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GAATTTGTCA ATCTCTATTC TGACTACATT CTCAATAAAT CAGTAGAAAA ACAGTTCAAA
    GCTTTTCGGA GAGGTTTTCA TATGGTGACC AACGAATCTC CTTTAAAGTA TTTATTCAGA
    CCAGAGGAAA TTGAATTGCT TATATGTGGA AGCCGGAATC TAGATTTCCA AGCACTAGAA
    GAAACTACAG AATATGACGG TGGCTATACC AGGGACTCTG TTCTGATTAG AGAGTTCTGG
    GAAATCGTTC ATTCATTTAC AGATGAGCAG AAAAGACTCT TCTTGCAGTT TACAACAGGC
    ACAGACAGAG CACCTGTGGG CGGACTAGGA AAATTAAAGA TGATTATAGC CAAAAATGGC
    CCAGACACAG AAAGGTTACC TACATCTCAT ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA
    TATTCAAGCA AAGAAAAACT TAAAGAAAGA TTGTTGAAGG CCATCACTTA CGCCAAAGGA
    TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272523.1
    CDS372..2966
    Translation

    Target ORF information:

    RefSeq Version XM_022416815.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416815.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGGAGG GTCGCACTTT CAAAAATGAG TCAGCTGGTA CATGGGGTTA TCATCAGTTT 
    TTAGCTCTTC TGTCTGAGAC ATACAAACAA GTTTGGAAGC AATCTTGGGG TACTTACCCA
    CAAGGCTGGT GGAGACCAGG TGTGTCACAA AGATCGCCAG GAGAACCTCA GTCTGACGAC
    ATCGAAGCTA GCCGAATGAA GCGAGCAGCT GCAAAGCATC TAATAGAACG CTACTACCAC
    CAGTTAACTG AGGGCTGTGG AAATGAGGCC TGCACGAATG AGTTTTGTGC TTCCTGTCCA
    ACTTTTCTTC GTATGGATAA CAATGCAGCA GCTATTAAAG CCCTCGAGCT TTATAAGATT
    AATGCAAAAC TCTGTGATCC TCATCCCTCC AAGAAAGGAG CAAGTTCAGC TTACCTTGAA
    AACTCGAAAG GTGCCCCTAA CAACTCCTGC TCTGACATAA AAATGAACAA GAAGGAAGGA
    CAAGGAGCAA GAGATGATTT TAGAGATGTG ACTTACCTAA CAGAAGAGAC GGTGTATGAA
    ATTCTTGAAT TATGTAGAGA GAGAGAGGAT TATTCCCCTT TAATCCGTGT TATTGGAAGA
    GTTTTTTCTA GTGCTGAGGC ATTGGTACAG AGCTTTCGGA AAGTCAAACA ACACACCAAG
    GAAGAACTGA AATCTCTTCA AGGAAAGGAT GAAGACAAGG ATGAAGATGA AAAGGAAAAA
    GCTGCATGTT CTGCTGCTAT GGAAGAAGAT TCAGAAGCAT CTTCTTCAAG GATAAGTGAT
    AGTTCACAAG GAGATAACAA CTTACAAAAA ATAGGCCCTG ATGATGTGTC TGTGGATATT
    GAGGCCATTA GAAGGGTCTA TACCAGATTA CTCTCTAATG AAAAAATAGA AACTGCTTTT
    CTCAATGCAC TTGTGTATTT GTCACCTAAT GTGGAATGTG ACTTGACGTA TCACAATGTA
    TACTCTCGAG ATCCTAATTA TCTGAATTTA TTCATTATTG TAATGGAGAA TAGAAATCTA
    CACAGTCCTG AATATCTGGA AATGGCTTTG CCATTGTTTT GCAAAGCAAT GAGTAAGCTA
    CCCCTTGCAG CCCAAGGAAA ATTGGTTAGA CTGTGGTCTA AATACAGTGC AGACCAAATT
    CGTAGAATGA TGGAAACATT TCAGCAACTT ATTACTTACA AAGTCATAAG CAATGAATTT
    AACAGTCGGA ATCTAGTGAA TGATGATGAT GCTATTGTTG CTGCTTCAAA GTGCTTGAAA
    ATGGTTTACT ATGCAAATGT AGTGGGTGGG GAAGTGGACA CAAATCACAA TGAAGAAGAT
    GATGAAGAGC CCATCCCTGA ATCCAGTGAA TTGACACTTC AGGAGCTTTT GGGAGAGGAA
    AGAAGAAATA AGAAAGGTCC TCGAGTGGAC CCATTGGAAA CTGAACTTGG TGTTAAAACT
    CTGGATTGTC GAAAACCACT TATCCCTTTT GAAGAGTTTA TTAATGAACC ACTGAATGAT
    GTTCTAGAAA TGGATAAAGA TTATACTTTT TTCAAAGTAG AAACAGAGAA CAAATTCTCT
    TTCATGACAT GTCCCTTTAT ATTGAATGCT GTCACAAAGA ATTTGGGATT ATATTATGAC
    AATAGAATTC GCATGTACAG TGAACGAAGA ATCACTGTTC TCTACAGCTT AGTTCAAGGA
    CAGCAGTTGA ATCCATATTT GAGACTCAAA GTCAGACGTG ACCATATCAT AGATGATGCA
    CTTGTCCGGC TAGAGATGAT CGCTATGGAA AATCCTGCAG ACTTGAAGAA ACAGTTGTAT
    GTGGAATTTG AAGGAGAACA AGGAGTTGAT GAGGGAGGTG TTTCCAAAGA ATTTTTTCAG
    CTGGTTGTGG AGGAAATCTT CAATCCAGAT ATTGGTATGT TCACATATGA TGAATCTACA
    AAATTATTTT GGTTTAATCC ATCCTCTTTT GAAACTGAGG GTCAGTTCAC TCTGATTGGC
    ATAGTGCTGG GTCTGGCTAT TTACAATAAC TGTATACTGG ATGTACATTT TCCCATGGTT
    GTCTACAGGA AGCTAATGGG GAAAAAAGGA ACTTTTCGTG ACCTAGGAGA CTCTCACCCT
    GAATTTGTCA ATCTCTATTC TGACTACATT CTCAATAAAT CAGTAGAAAA ACAGTTCAAA
    GCTTTTCGGA GAGGTTTTCA TATGGTGACC AACGAATCTC CTTTAAAGTA TTTATTCAGA
    CCAGAGGAAA TTGAATTGCT TATATGTGGA AGCCGGAATC TAGATTTCCA AGCACTAGAA
    GAAACTACAG AATATGACGG TGGCTATACC AGGGACTCTG TTCTGATTAG AGAGTTCTGG
    GAAATCGTTC ATTCATTTAC AGATGAGCAG AAAAGACTCT TCTTGCAGTT TACAACAGGC
    ACAGACAGAG CACCTGTGGG CGGACTAGGA AAATTAAAGA TGATTATAGC CAAAAATGGC
    CCAGACACAG AAAGGTTACC TACATCTCAT ACTTGCTTTA ATGTCCTTTT ACTGCCGGAA
    TATTCAAGCA AAGAAAAACT TAAAGAAAGA TTGTTGAAGG CCATCACTTA CGCCAAAGGA
    TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.