UBE3A cDNA ORF clone, Bos taurus(cattle)

The following UBE3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa37739 NM_001098462.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30767 XM_005221700.4
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30767 XM_024981861.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30767 XM_015459142.2
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_005221701.4
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_024981862.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_024981863.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_024981864.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_010816882.3
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa228368 XM_024981865.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa228369 XM_024981866.1
Latest version!
Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OBa30767 XM_005221700.3 Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_010816882.2 Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30768 XM_005221701.3 Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30767 XM_015459142.1 Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa37739
    Clone ID Related Accession (Same CDS sequence) NM_001098462.1
    Accession Version NM_001098462.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134678.1. On Jun 5, 2007 this sequence version replaced XM_612444.3. ##Evidence-Data-START## Transcript exon combination :: BC134678.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT TAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091932.1
    CDS161..2788
    Translation

    Target ORF information:

    RefSeq Version NM_001098462.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098462.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT TAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30767
    Clone ID Related Accession (Same CDS sequence) XM_005221700.3 , XM_015459142.1 , XM_005221700.4 , XM_024981861.1 , XM_015459142.2
    Accession Version XM_005221700.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104528.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221757.1
    CDS249..2876
    Translation

    Target ORF information:

    RefSeq Version XM_005221700.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221700.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30767
    Clone ID Related Accession (Same CDS sequence) XM_005221700.3 , XM_015459142.1 , XM_005221700.4 , XM_024981861.1 , XM_015459142.2
    Accession Version XM_015459142.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104528.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314628.1
    CDS187..2814
    Translation

    Target ORF information:

    RefSeq Version XM_015459142.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30767
    Clone ID Related Accession (Same CDS sequence) XM_005221700.3 , XM_015459142.1 , XM_005221700.4 , XM_024981861.1 , XM_015459142.2
    Accession Version XM_005221700.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221700.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221757.1
    CDS388..3015
    Translation

    Target ORF information:

    RefSeq Version XM_005221700.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221700.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30767
    Clone ID Related Accession (Same CDS sequence) XM_005221700.3 , XM_015459142.1 , XM_005221700.4 , XM_024981861.1 , XM_015459142.2
    Accession Version XM_024981861.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837629.1
    CDS216..2843
    Translation

    Target ORF information:

    RefSeq Version XM_024981861.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30767
    Clone ID Related Accession (Same CDS sequence) XM_005221700.3 , XM_015459142.1 , XM_005221700.4 , XM_024981861.1 , XM_015459142.2
    Accession Version XM_015459142.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314628.1
    CDS280..2907
    Translation

    Target ORF information:

    RefSeq Version XM_015459142.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459142.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
    CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG
    GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_005221701.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104528.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221758.1
    CDS512..3076
    Translation

    Target ORF information:

    RefSeq Version XM_005221701.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221701.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_010816882.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104528.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815184.1
    CDS122..2686
    Translation

    Target ORF information:

    RefSeq Version XM_010816882.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_010816882.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815184.1
    CDS588..3152
    Translation

    Target ORF information:

    RefSeq Version XM_010816882.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816882.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_024981863.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837631.1
    CDS414..2978
    Translation

    Target ORF information:

    RefSeq Version XM_024981863.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981863.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_005221701.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221701.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221758.1
    CDS623..3187
    Translation

    Target ORF information:

    RefSeq Version XM_005221701.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221701.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_024981862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837630.1
    CDS543..3107
    Translation

    Target ORF information:

    RefSeq Version XM_024981862.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30768
    Clone ID Related Accession (Same CDS sequence) XM_005221701.3 , XM_010816882.2 , XM_010816882.3 , XM_024981863.1 , XM_005221701.4 , XM_024981862.1 , XM_024981864.1
    Accession Version XM_024981864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837632.1
    CDS223..2787
    Translation

    Target ORF information:

    RefSeq Version XM_024981864.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGCGAG CAGCTGCAAA ACATCTAATA GAACGCTACT ACCACCAGTT AACTGAGGGC 
    TGTGGAAATG AGGCCTGCAC GAATGAGTTT TGTGCTTCCT GTCCAACTTT TCGTCGTATG
    GATAACAATG CAGCAGCTAT TAAAGCCCTC GAGCTTTATA AGATTAATGC AAAACTCTGT
    GATCCTCATC CCTCCAAGAA AGGAGCAAGC TCAGCTTACC TTGAAAACTC TAAAGGTGCC
    TCTAACAACT GCTCTGACAT AAAAATGAAC AAGAAGGACG GGCAAGGAGC AAGAGATGAT
    TTTAAAGATG TGACTTACCT AACCGAAGAG ATGGTGTATG AAATTCTTGA ATTATGTAGA
    GAGAGAGAGG ATTATTCCCC TTTAATTCGT GTTATTGGAA GAGTTTTTTC TAGTGCTGAA
    GCATTGGTAC AAAGCTTTCG GAAAGTTAAG CAACACACCA AGGAAGAACT GAAATCTCTT
    CAAGGAAAGG ATGAAGATAA AGATGAAGAC GAAAAGGAAA AGGCTGCATG TTCCGCAGCA
    GCTATGGAGG AGGATTCAGA AGCATCTTCC TCAAGGATAA GTGATAGCTC ACAAGGAGAT
    AACAACTTAC AAAAGTTAGG CCCTGATGAT GTGTCTGTGG ATATTGAGGC TATTAGAAGG
    GTCTACACCA GATTGCTCTC TAATGAAAAA ATAGAAACTG CTTTTCTCAA TGCACTTGTG
    TATTTGTCAC CTAATGTGGA ATGTGACTTG ACGTATCACA ATGTATACTC CCGAGATCCT
    AATTATCTGA ATTTATTCAT TATTGTCATG GAGAACAGAA ATCTCCACAG TCCTGAATAT
    CTGGAAATGG CTTTGCCACT ATTTTGCAAA GCAATGAGTA AGCTACCCCT TGCAGCCCAA
    GGAAAACTGG TCAGACTGTG GTCTAAATAC AGTGCAGACC AGATTCGGAG AATGATGGAA
    ACATTTCAGC AGCTTATTAC ATACAAAGTC ATAAGCACTG ACTTTAACAA TCGAAATCTA
    GTGAACGATG ATGATGCTAT TGTTGCTGCT TCCAAGTGCT TGAAAATGGT TTACTATGCA
    AATGTAGTAG GTGGGGAGGT AGACACAAAT CATAATGAAG AAGATGATGA AGAGCCCCTT
    CCTGAGTCCA GTGAATTGAC ACTTCAGGAG TTATTGGGAG AAGAAAGAAG GAACAAGAAA
    GGTCCTCGAG TGGACCCACT GGAAACTGAA CTTGCTGTTA AAACTCTGGA TTGTCGAAAA
    CCACTTATCC CTTTTGAAGA GTTTATTAAT GAACCACTGA ATGATGTTCT AGAAATGGAT
    AAAGATTATA CTTTTTTCAA AGTAGAAACA GAGAACAAGT TCTCTTTTAT GACATGTCCC
    TTTATACTGA ATGCTGTCAC AAAGAATTTG GGATTATATT ATGACAATAG AATTCGCATG
    TACAGTGAAC GAAGAATTAC TGTTCTCTAC AGCTTAGTTC AAGGACAGCA GTTGAATCCA
    TATTTGAGAC TCAAAGTCAG ACGTGACCAT ATCATAGATG ATGCACTTGT CCGGCTAGAG
    ATGATCGCTA TGGAAAATCC TGCAGACTTG AAGAAACAGT TGTATGTGGA ATTTGAAGGA
    GAACAAGGAG TTGATGAGGG AGGTGTTTCC AAAGAATTTT TTCAGCTGGT TGTGGAGGAA
    ATCTTCAATC CAGATATTGG TATGTTCACA TATGATGAAT CTACAAAGTT GTTTTGGTTT
    AATCCATCCT CTTTTGAAAC TGAGGGTCAG TTCACTCTGA TTGGCATAGT ACTGGGTTTG
    GCTATTTACA ATAACTGTAT ACTGGATGTA CATTTTCCCA TGGTTGTCTA CAGGAAGCTA
    ATGGGGAAAA AAGGAACTTT TCTTGACTTG GGAGACTCTC ATCCTGTTCT GTATCAGAGT
    TTAAAAGACT TACTGGAGTA TGAAGGGAAT GTGGAAGATG ATATGATGAT CACTTTCCAG
    ATATCTCAGA CAGATCTTTT TGGTAATCCG ATGATGTATG ATCTAAAGGA AAATGGTGAT
    AAAATTCCAA TTACCAATGA AAACAGAAAG GAATTTGTCA ATCTGTATTC TGACTACATT
    CTCAATAAAT CAGTAGAAAA ACAGTTCAAG GCTTTTCGGA GAGGTTTTCA TATGGTGACC
    AATGAATCTC CTTTAAAGTA CTTATTCAGA CCAGAGGAAA TTGAATTGCT TATATGTGGA
    AGCCGGAATC TAGATTTCCA AGCACTAGAA GAAACTACAG AATATGACGG TGGCTATACC
    AGGGACTCTG TTGTGATTAG GGAGTTCTGG GAAATTGTTC ATTCATTTAC AGATGAGCAG
    AAAAGACTCT TCTTGCAGTT TACAACAGGC ACAGACAGGG CACCTGTGGG CGGACTAGGA
    AAATTGAAGA TGATAATAGC CAAAAATGGT CCAGACACAG AAAGGTTACC TACATCTCAT
    ACTTGCTTTA ATGTCCTTTT ACTTCCGGAA TATTCAAGCA AAGAAAAACT TAAAGAGAGA
    TTGTTGAAGG CCATCACTTA TGCCAAAGGA TTTGGCATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228368
    Clone ID Related Accession (Same CDS sequence) XM_024981865.1
    Accession Version XM_024981865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837633.1
    CDS231..2678
    Translation

    Target ORF information:

    RefSeq Version XM_024981865.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
    TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT
    GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT
    GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT
    AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT
    GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT
    CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA
    GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA
    GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA
    AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT
    GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT
    CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA
    TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC
    CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG
    GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT
    CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT
    GCAAATGTAG TAGGTGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC
    CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG
    AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA
    AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG
    GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT
    CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC
    ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT
    CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA
    GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA
    GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG
    GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG
    TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT
    TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG
    CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG
    AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC
    CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT
    GATAAAATTC CAATTACCAA TGAAAACAGA AAGGAATTTG TCAATCTGTA TTCTGACTAC
    ATTCTCAATA AATCAGTAGA AAAACAGTTC AAGGCTTTTC GGAGAGGTTT TCATATGGTG
    ACCAATGAAT CTCCTTTAAA GTACTTATTC AGACCAGAGG AAATTGAATT GCTTATATGT
    GGAAGCCGGA ATCTAGATTT CCAAGCACTA GAAGAAACTA CAGAATATGA CGGTGGCTAT
    ACCAGGGACT CTGTTGTGAT TAGGGAGTTC TGGGAAATTG TTCATTCATT TACAGATGAG
    CAGAAAAGAC TCTTCTTGCA GTTTACAACA GGCACAGACA GGGCACCTGT GGGCGGACTA
    GGAAAATTGA AGATGATAAT AGCCAAAAAT GGTCCAGACA CAGAAAGGTT ACCTACATCT
    CATACTTGCT TTAATGTCCT TTTACTTCCG GAATATTCAA GCAAAGAAAA ACTTAAAGAG
    AGATTGTTGA AGGCCATCAC TTATGCCAAA GGATTTGGCA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228369
    Clone ID Related Accession (Same CDS sequence) XM_024981866.1
    Accession Version XM_024981866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin-protein ligase E3A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACAGCCC TGCCCAGAGA TGTGACTTAC CTAACCGAAG AGATGGTGTA TGAAATTCTT 
    GAATTATGTA GAGAGAGAGA GGATTATTCC CCTTTAATTC GTGTTATTGG AAGAGTTTTT
    TCTAGTGCTG AAGCATTGGT ACAAAGCTTT CGGAAAGTTA AGCAACACAC CAAGGAAGAA
    CTGAAATCTC TTCAAGGAAA GGATGAAGAT AAAGATGAAG ACGAAAAGGA AAAGGCTGCA
    TGTTCCGCAG CAGCTATGGA GGAGGATTCA GAAGCATCTT CCTCAAGGAT AAGTGATAGC
    TCACAAGGAG ATAACAACTT ACAAAAGTTA GGCCCTGATG ATGTGTCTGT GGATATTGAG
    GCTATTAGAA GGGTCTACAC CAGATTGCTC TCTAATGAAA AAATAGAAAC TGCTTTTCTC
    AATGCACTTG TGTATTTGTC ACCTAATGTG GAATGTGACT TGACGTATCA CAATGTATAC
    TCCCGAGATC CTAATTATCT GAATTTATTC ATTATTGTCA TGGAGAACAG AAATCTCCAC
    AGTCCTGAAT ATCTGGAAAT GGCTTTGCCA CTATTTTGCA AAGCAATGAG TAAGCTACCC
    CTTGCAGCCC AAGGAAAACT GGTCAGACTG TGGTCTAAAT ACAGTGCAGA CCAGATTCGG
    AGAATGATGG AAACATTTCA GCAGCTTATT ACATACAAAG TCATAAGCAC TGACTTTAAC
    AATCGAAATC TAGTGAACGA TGATGATGCT ATTGTTGCTG CTTCCAAGTG CTTGAAAATG
    GTTTACTATG CAAATGTAGT AGGTGGGGAG GTAGACACAA ATCATAATGA AGAAGATGAT
    GAAGAGCCCC TTCCTGAGTC CAGTGAATTG ACACTTCAGG AGTTATTGGG AGAAGAAAGA
    AGGAACAAGA AAGGTCCTCG AGTGGACCCA CTGGAAACTG AACTTGCTGT TAAAACTCTG
    GATTGTCGAA AACCACTTAT CCCTTTTGAA GAGTTTATTA ATGAACCACT GAATGATGTT
    CTAGAAATGG ATAAAGATTA TACTTTTTTC AAAGTAGAAA CAGAGAACAA GTTCTCTTTT
    ATGACATGTC CCTTTATACT GAATGCTGTC ACAAAGAATT TGGGATTATA TTATGACAAT
    AGAATTCGCA TGTACAGTGA ACGAAGAATT ACTGTTCTCT ACAGCTTAGT TCAAGGACAG
    CAGTTGAATC CATATTTGAG ACTCAAAGTC AGACGTGACC ATATCATAGA TGATGCACTT
    GTCCGGCTAG AGATGATCGC TATGGAAAAT CCTGCAGACT TGAAGAAACA GTTGTATGTG
    GAATTTGAAG GAGAACAAGG AGTTGATGAG GGAGGTGTTT CCAAAGAATT TTTTCAGCTG
    GTTGTGGAGG AAATCTTCAA TCCAGATATT GGTATGTTCA CATATGATGA ATCTACAAAG
    TTGTTTTGGT TTAATCCATC CTCTTTTGAA ACTGAGGGTC AGTTCACTCT GATTGGCATA
    GTACTGGGTT TGGCTATTTA CAATAACTGT ATACTGGATG TACATTTTCC CATGGTTGTC
    TACAGGAAGC TAATGGGGAA AAAAGGAACT TTTCTTGACT TGGGAGACTC TCATCCTGTT
    CTGTATCAGA GTTTAAAAGA CTTACTGGAG TATGAAGGGA ATGTGGAAGA TGATATGATG
    ATCACTTTCC AGATATCTCA GACAGATCTT TTTGGTAATC CGATGATGTA TGATCTAAAG
    GAAAATGGTG ATAAAATTCC AATTACCAAT GAAAACAGAA AGGAATTTGT CAATCTGTAT
    TCTGACTACA TTCTCAATAA ATCAGTAGAA AAACAGTTCA AGGCTTTTCG GAGAGGTTTT
    CATATGGTGA CCAATGAATC TCCTTTAAAG TACTTATTCA GACCAGAGGA AATTGAATTG
    CTTATATGTG GAAGCCGGAA TCTAGATTTC CAAGCACTAG AAGAAACTAC AGAATATGAC
    GGTGGCTATA CCAGGGACTC TGTTGTGATT AGGGAGTTCT GGGAAATTGT TCATTCATTT
    ACAGATGAGC AGAAAAGACT CTTCTTGCAG TTTACAACAG GCACAGACAG GGCACCTGTG
    GGCGGACTAG GAAAATTGAA GATGATAATA GCCAAAAATG GTCCAGACAC AGAAAGGTTA
    CCTACATCTC ATACTTGCTT TAATGTCCTT TTACTTCCGG AATATTCAAG CAAAGAAAAA
    CTTAAAGAGA GATTGTTGAA GGCCATCACT TATGCCAAAG GATTTGGCAT GCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837634.1
    CDS150..2426
    Translation

    Target ORF information:

    RefSeq Version XM_024981866.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin protein ligase E3A (UBE3A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACAGCCC TGCCCAGAGA TGTGACTTAC CTAACCGAAG AGATGGTGTA TGAAATTCTT 
    GAATTATGTA GAGAGAGAGA GGATTATTCC CCTTTAATTC GTGTTATTGG AAGAGTTTTT
    TCTAGTGCTG AAGCATTGGT ACAAAGCTTT CGGAAAGTTA AGCAACACAC CAAGGAAGAA
    CTGAAATCTC TTCAAGGAAA GGATGAAGAT AAAGATGAAG ACGAAAAGGA AAAGGCTGCA
    TGTTCCGCAG CAGCTATGGA GGAGGATTCA GAAGCATCTT CCTCAAGGAT AAGTGATAGC
    TCACAAGGAG ATAACAACTT ACAAAAGTTA GGCCCTGATG ATGTGTCTGT GGATATTGAG
    GCTATTAGAA GGGTCTACAC CAGATTGCTC TCTAATGAAA AAATAGAAAC TGCTTTTCTC
    AATGCACTTG TGTATTTGTC ACCTAATGTG GAATGTGACT TGACGTATCA CAATGTATAC
    TCCCGAGATC CTAATTATCT GAATTTATTC ATTATTGTCA TGGAGAACAG AAATCTCCAC
    AGTCCTGAAT ATCTGGAAAT GGCTTTGCCA CTATTTTGCA AAGCAATGAG TAAGCTACCC
    CTTGCAGCCC AAGGAAAACT GGTCAGACTG TGGTCTAAAT ACAGTGCAGA CCAGATTCGG
    AGAATGATGG AAACATTTCA GCAGCTTATT ACATACAAAG TCATAAGCAC TGACTTTAAC
    AATCGAAATC TAGTGAACGA TGATGATGCT ATTGTTGCTG CTTCCAAGTG CTTGAAAATG
    GTTTACTATG CAAATGTAGT AGGTGGGGAG GTAGACACAA ATCATAATGA AGAAGATGAT
    GAAGAGCCCC TTCCTGAGTC CAGTGAATTG ACACTTCAGG AGTTATTGGG AGAAGAAAGA
    AGGAACAAGA AAGGTCCTCG AGTGGACCCA CTGGAAACTG AACTTGCTGT TAAAACTCTG
    GATTGTCGAA AACCACTTAT CCCTTTTGAA GAGTTTATTA ATGAACCACT GAATGATGTT
    CTAGAAATGG ATAAAGATTA TACTTTTTTC AAAGTAGAAA CAGAGAACAA GTTCTCTTTT
    ATGACATGTC CCTTTATACT GAATGCTGTC ACAAAGAATT TGGGATTATA TTATGACAAT
    AGAATTCGCA TGTACAGTGA ACGAAGAATT ACTGTTCTCT ACAGCTTAGT TCAAGGACAG
    CAGTTGAATC CATATTTGAG ACTCAAAGTC AGACGTGACC ATATCATAGA TGATGCACTT
    GTCCGGCTAG AGATGATCGC TATGGAAAAT CCTGCAGACT TGAAGAAACA GTTGTATGTG
    GAATTTGAAG GAGAACAAGG AGTTGATGAG GGAGGTGTTT CCAAAGAATT TTTTCAGCTG
    GTTGTGGAGG AAATCTTCAA TCCAGATATT GGTATGTTCA CATATGATGA ATCTACAAAG
    TTGTTTTGGT TTAATCCATC CTCTTTTGAA ACTGAGGGTC AGTTCACTCT GATTGGCATA
    GTACTGGGTT TGGCTATTTA CAATAACTGT ATACTGGATG TACATTTTCC CATGGTTGTC
    TACAGGAAGC TAATGGGGAA AAAAGGAACT TTTCTTGACT TGGGAGACTC TCATCCTGTT
    CTGTATCAGA GTTTAAAAGA CTTACTGGAG TATGAAGGGA ATGTGGAAGA TGATATGATG
    ATCACTTTCC AGATATCTCA GACAGATCTT TTTGGTAATC CGATGATGTA TGATCTAAAG
    GAAAATGGTG ATAAAATTCC AATTACCAAT GAAAACAGAA AGGAATTTGT CAATCTGTAT
    TCTGACTACA TTCTCAATAA ATCAGTAGAA AAACAGTTCA AGGCTTTTCG GAGAGGTTTT
    CATATGGTGA CCAATGAATC TCCTTTAAAG TACTTATTCA GACCAGAGGA AATTGAATTG
    CTTATATGTG GAAGCCGGAA TCTAGATTTC CAAGCACTAG AAGAAACTAC AGAATATGAC
    GGTGGCTATA CCAGGGACTC TGTTGTGATT AGGGAGTTCT GGGAAATTGT TCATTCATTT
    ACAGATGAGC AGAAAAGACT CTTCTTGCAG TTTACAACAG GCACAGACAG GGCACCTGTG
    GGCGGACTAG GAAAATTGAA GATGATAATA GCCAAAAATG GTCCAGACAC AGAAAGGTTA
    CCTACATCTC ATACTTGCTT TAATGTCCTT TTACTTCCGG AATATTCAAG CAAAGAAAAA
    CTTAAAGAGA GATTGTTGAA GGCCATCACT TATGCCAAAG GATTTGGCAT GCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.