nfat5 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004913614.3	XP_004913671.1	nuclear factor of activated T-cells 5 isoform X3
XM_004913618.3	XP_004913675.1	nuclear factor of activated T-cells 5 isoform X5
XM_004913620.3	XP_004913677.1	nuclear factor of activated T-cells 5 isoform X6
XM_004913615.4	XP_004913672.1	nuclear factor of activated T-cells 5 isoform X4
XM_004913617.4	XP_004913674.1	nuclear factor of activated T-cells 5 isoform X4
XM_004913618.4	XP_004913675.2	nuclear factor of activated T-cells 5 isoform X5
XM_018093513.2	XP_017949002.1	nuclear factor of activated T-cells 5 isoform X6
XM_031900792.1	XP_031756652.1	nuclear factor of activated T-cells 5 isoform X4
XM_012962319.2	XP_012817773.1	nuclear factor of activated T-cells 5 isoform X6
XM_004913622.3	XP_004913679.1	nuclear factor of activated T-cells 5 isoform X7
XM_012962324.2	XP_012817778.1	nuclear factor of activated T-cells 5 isoform X7
XM_004913616.4	XP_004913673.1	nuclear factor of activated T-cells 5 isoform X4
XM_012962322.3	XP_012817776.1	nuclear factor of activated T-cells 5 isoform X6
XM_012962323.3	XP_012817777.1	nuclear factor of activated T-cells 5 isoform X7
XM_018093511.1	XP_017949000.1	nuclear factor of activated T-cells 5 isoform X4
XM_004913621.3	XP_004913678.1	nuclear factor of activated T-cells 5 isoform X7
XM_004913622.4	XP_004913679.1	nuclear factor of activated T-cells 5 isoform X7
XM_012962324.3	XP_012817778.1	nuclear factor of activated T-cells 5 isoform X7
XM_002937577.4	XP_002937623.2	nuclear factor of activated T-cells 5 isoform X1
XM_004913615.3	XP_004913672.1	nuclear factor of activated T-cells 5 isoform X4
XM_004913616.3	XP_004913673.1	nuclear factor of activated T-cells 5 isoform X4
XM_012962322.2	XP_012817776.1	nuclear factor of activated T-cells 5 isoform X6
XM_002937577.5	XP_002937623.3	nuclear factor of activated T-cells 5 isoform X1
XM_004913620.4	XP_004913677.1	nuclear factor of activated T-cells 5 isoform X6
XM_004913613.3	XP_004913670.1	nuclear factor of activated T-cells 5 isoform X2
XM_004913617.3	XP_004913674.1	nuclear factor of activated T-cells 5 isoform X4
XM_018093513.1	XP_017949002.1	nuclear factor of activated T-cells 5 isoform X6
XM_012962323.2	XP_012817777.1	nuclear factor of activated T-cells 5 isoform X7
XM_004913613.4	XP_004913670.2	nuclear factor of activated T-cells 5 isoform X2
XM_004913614.4	XP_004913671.2	nuclear factor of activated T-cells 5 isoform X3
XM_018093511.2	XP_017949000.1	nuclear factor of activated T-cells 5 isoform X4
XM_012962319.3	XP_012817773.1	nuclear factor of activated T-cells 5 isoform X6
XM_004913621.4	XP_004913678.1	nuclear factor of activated T-cells 5 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa56880	XM_002937577.5 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56881	XM_004913613.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56882	XM_004913614.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56883	XM_031900792.1 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56883	XM_004913615.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56883	XM_018093511.2 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56883	XM_004913617.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56883	XM_004913616.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56884	XM_004913618.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56885	XM_004913620.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56885	XM_012962319.3 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56885	XM_012962322.3 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56885	XM_018093513.2 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa56886	XM_004913622.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56886	XM_012962324.3 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56886	XM_012962323.3 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56886	XM_004913621.4 Latest version!	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa08901	XM_004913622.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08901	XM_004913621.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08901	XM_012962323.2 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08901	XM_012962324.2 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08895	XM_002937577.4 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08896	XM_004913613.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08897	XM_004913614.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08898	XM_004913615.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08898	XM_004913616.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08898	XM_004913617.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08898	XM_018093511.1 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08899	XM_004913618.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08900	XM_004913620.3 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08900	XM_018093513.1 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08900	XM_012962322.2 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa08900	XM_012962319.2 Latest version!	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

nfat5 ( XM_002937577.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937623.3 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa56880
Clone ID Related Accession (Same CDS sequence)	XM_002937577.5
Accession Version	XM_002937577.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937577.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAGTG 
AGTTTGCTCC CAAGTACTCT GGGTTCTCCT CTTAAATCAA GTGGTTTAAT CAAAAATGAA 
GATAGTGCTC CTATGGAGAT GGCTGCTGAT CAGAGATCCT CTCCTCTATA CAAAACAACA 
ACCATTGTAT CAGCTCAGCA AAATCTAGAA AATTCATCAA ATGCTAGACC TTTTTCACCC 
ACTGTATCCC ATCTTCCCAC TGATGCTGAC CAACAGGCAT CAATTCAAGT GCAGACATTC 
AGCACAGAAC CTCTGGCCAC CATCCAGACC CAAGACATTG CCCAGCCTGG TAGTTTTCAG 
TCAGTGACAT CTCCGGGCCA GGCTAATGAT TCCATAATGC AGCAGACTGG ACAATTTCAA 
ACTATGGATG GCCAACCCAG GGACACAATG CAGCCAGAAA ACGCCATTGT CACACTTTCA 
CAAATGGCTG AAGTTACTCC ACAAGTGCAG CAGTCCACAA TGCAAGAGCA AGCGCAGACA 
TTACAACAGC AGATTACTAC AGGCTTATTC ACCTCCACAA GTAGCGTTAA CCAGTTACAG 
AGTGCTATTA ACCAACTACA GGCTGGTAGC TTTCAGTCTA GTGTAAGTGG TGGTAATACA 
AATGTAGATT TGGTCCAGCA GGTTCTGGAC TCCCAGCAGT TGTCTTGCGT ACTTTTTTCT 
GGACAAAGCG GAAATGAAAG CAAGCAGGCC TCAGTTGGAC AAGTTCAGGA GCGGATCAGT 
GCTGGCATAT TCCAACCTGT TGCTTCAATA CAAAACAATG CAAATTCTGC ACTGTTTTCC 
ACCCCTGAAT CTGTGCATTC CAGACCAGAA AACGTGCTTT CGGGAAGAAC TGACAGCCTT 
CTCCAGCAGG CTGAAAATGC TTTGTCTAAT CAACAGCAGC CCATGGAGAC CACAGCAGCC 
CTAGTCATGG AGATGCAGCA AAGTATTCTT CCCTCTGCTG CAACGATTCA GCCTGACCTT 
TTCCAGACAC AAAACTCTGG AACATCAAAC ATTCAGTCAC CTGTCTATCA GCACCAGCAA 
GGTTCTCATT TAATCCCTGG ACTGTCCACT AATGAAGAGA TGCCAATGCA GTGTGATATG 
TTTGCTAGTG CAGCAGTTCC TGGAAGTGAA AGCAGTGCAA ACCAGCAGCA GGTATCTTCA 
TCTAGTTCAA ATATGTTCCA GCCCACAAGC TCTACAGAAA ATGATAATGC TTCTGCACAG 
GCAGAGCACA TCCAAAGGAA TGTGTTTCAA TCTATGGTCC AGATGCAACA CAGCGGAGAA 
ACCCAGAATC AAGTCAGTCT TTTTTCACCA GATGAAGACA TGCTTGGAGT TCAGACAAGT 
GTAAGCCAAC AGCAAGGCAG TGGGCTTTTT CCCCCTGGAG ACTTAATGAC TCTTCAGTCT 
AGTAACTTCA TGCAACAATC CCCTCACTCA CACACTACAA TTTATCACTC ACAGAACTCT 
ATTGGGGAGA GCCAGAGTAT AAACCAGGAA ACTCAGGGCT CTCTCTTCCA CTCTACCAAC 
ACTATGGTGC AGCATCAGAA CACTGCATCT CAAGATCAGC TTCAGTCTCC ACTTTTCCAC 
CCACAGAACA CATTGTCTGT TTTGCAGAGT TCCTCTGTCA CTGCAGAGCA GACCTCGTCT 
AACCTCTTCC TGTCTCAAAG CACTATGAGC TCGCTGCAAA GTAATCCAGT GTCTCAAGAG 
AAGCAGCTCT CCTTTTTCAC AAGTCAAAAT TCACTCCCTC AGCTCCAAGC TGCTCCAACC 
ACAGAACAGC AAGCAGCCTT TCAACAGCAA GCCCAACTGT CTCACATTCA AAACTCAATC 
ATATCCCAAG AACAGCAGCA ACAAGTTTCA CAAAATATAT ATCAGCCACA GAACCAACAA 
ACAGCAATTT TCCAGGCAAC TCACTCAATG GTTGGGATTC AGGGTAGTCC ATCTTCTCAT 
GAGCAGAGAC AAAACATCCT ATTCAGCACA CAAAATTCAC TGTCTAACAT GGGATCGCCA 
GAGCAGCAGC AAAGCATGCT TTTTAATCCT GGACAGGAGC CAATGTCCAC CCAGGAGCAG 
CAGAACCAAG CTCTCTTTCA TTCCCAGAAC ATGGAGTTCC AGTCTCAGAG CTCTGTTTCC 
ACTCAGCATG AACAGCAGCA GCAGTCTCCA ATGTTTCATA ATTCTCCGCA ACTTCAGCTT 
GTTCAGGCCT CCACAACCTC TCCAGAGCAA CAGGTTACAT TGTTTATGTC CCCCGTTTCC 
ATGTCAGCCT TGCAGAGCAG CATCAACCAG CAAGACCTTC AGCAAGCCAG TATATACTCA 
CAGTCTATCC AGGGCAGTAC ACCACCTCCT CCCCAGCAGT CTAATATATA CCACAGTTCC 
ACAAGTGGAG CAATGGGCCA AATTCAAGGT CAGCCAACAT CATCACCGCA GGCATCAGGA 
ATGTTTTTGT TCGGGATTCA AAGCAATTGC GGTCAGCTGA TCTCCCAGGC TGCTGCTTCC 
ATACCTGATC AGATAATGGT TATGGCACAA TCAGGTCAAT CGCAAGGAGA TGGTCAATCT 
GTAACAGCAA TTCTATCCCA ACAAATGACA GATAATTCAT CAATGACATC TGAACAGAGC 
ATGGAGAAGA TCAGTGATTT GCTAGTTTCT CTGCAGAACC AGGGGACCAG TTTGTCTGGC 
TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937623.3
CDS	281..4669
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSQETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_002937577.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937577.5

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAGTG 
AGTTTGCTCC CAAGTACTCT GGGTTCTCCT CTTAAATCAA GTGGTTTAAT CAAAAATGAA 
GATAGTGCTC CTATGGAGAT GGCTGCTGAT CAGAGATCCT CTCCTCTATA CAAAACAACA 
ACCATTGTAT CAGCTCAGCA AAATCTAGAA AATTCATCAA ATGCTAGACC TTTTTCACCC 
ACTGTATCCC ATCTTCCCAC TGATGCTGAC CAACAGGCAT CAATTCAAGT GCAGACATTC 
AGCACAGAAC CTCTGGCCAC CATCCAGACC CAAGACATTG CCCAGCCTGG TAGTTTTCAG 
TCAGTGACAT CTCCGGGCCA GGCTAATGAT TCCATAATGC AGCAGACTGG ACAATTTCAA 
ACTATGGATG GCCAACCCAG GGACACAATG CAGCCAGAAA ACGCCATTGT CACACTTTCA 
CAAATGGCTG AAGTTACTCC ACAAGTGCAG CAGTCCACAA TGCAAGAGCA AGCGCAGACA 
TTACAACAGC AGATTACTAC AGGCTTATTC ACCTCCACAA GTAGCGTTAA CCAGTTACAG 
AGTGCTATTA ACCAACTACA GGCTGGTAGC TTTCAGTCTA GTGTAAGTGG TGGTAATACA 
AATGTAGATT TGGTCCAGCA GGTTCTGGAC TCCCAGCAGT TGTCTTGCGT ACTTTTTTCT 
GGACAAAGCG GAAATGAAAG CAAGCAGGCC TCAGTTGGAC AAGTTCAGGA GCGGATCAGT 
GCTGGCATAT TCCAACCTGT TGCTTCAATA CAAAACAATG CAAATTCTGC ACTGTTTTCC 
ACCCCTGAAT CTGTGCATTC CAGACCAGAA AACGTGCTTT CGGGAAGAAC TGACAGCCTT 
CTCCAGCAGG CTGAAAATGC TTTGTCTAAT CAACAGCAGC CCATGGAGAC CACAGCAGCC 
CTAGTCATGG AGATGCAGCA AAGTATTCTT CCCTCTGCTG CAACGATTCA GCCTGACCTT 
TTCCAGACAC AAAACTCTGG AACATCAAAC ATTCAGTCAC CTGTCTATCA GCACCAGCAA 
GGTTCTCATT TAATCCCTGG ACTGTCCACT AATGAAGAGA TGCCAATGCA GTGTGATATG 
TTTGCTAGTG CAGCAGTTCC TGGAAGTGAA AGCAGTGCAA ACCAGCAGCA GGTATCTTCA 
TCTAGTTCAA ATATGTTCCA GCCCACAAGC TCTACAGAAA ATGATAATGC TTCTGCACAG 
GCAGAGCACA TCCAAAGGAA TGTGTTTCAA TCTATGGTCC AGATGCAACA CAGCGGAGAA 
ACCCAGAATC AAGTCAGTCT TTTTTCACCA GATGAAGACA TGCTTGGAGT TCAGACAAGT 
GTAAGCCAAC AGCAAGGCAG TGGGCTTTTT CCCCCTGGAG ACTTAATGAC TCTTCAGTCT 
AGTAACTTCA TGCAACAATC CCCTCACTCA CACACTACAA TTTATCACTC ACAGAACTCT 
ATTGGGGAGA GCCAGAGTAT AAACCAGGAA ACTCAGGGCT CTCTCTTCCA CTCTACCAAC 
ACTATGGTGC AGCATCAGAA CACTGCATCT CAAGATCAGC TTCAGTCTCC ACTTTTCCAC 
CCACAGAACA CATTGTCTGT TTTGCAGAGT TCCTCTGTCA CTGCAGAGCA GACCTCGTCT 
AACCTCTTCC TGTCTCAAAG CACTATGAGC TCGCTGCAAA GTAATCCAGT GTCTCAAGAG 
AAGCAGCTCT CCTTTTTCAC AAGTCAAAAT TCACTCCCTC AGCTCCAAGC TGCTCCAACC 
ACAGAACAGC AAGCAGCCTT TCAACAGCAA GCCCAACTGT CTCACATTCA AAACTCAATC 
ATATCCCAAG AACAGCAGCA ACAAGTTTCA CAAAATATAT ATCAGCCACA GAACCAACAA 
ACAGCAATTT TCCAGGCAAC TCACTCAATG GTTGGGATTC AGGGTAGTCC ATCTTCTCAT 
GAGCAGAGAC AAAACATCCT ATTCAGCACA CAAAATTCAC TGTCTAACAT GGGATCGCCA 
GAGCAGCAGC AAAGCATGCT TTTTAATCCT GGACAGGAGC CAATGTCCAC CCAGGAGCAG 
CAGAACCAAG CTCTCTTTCA TTCCCAGAAC ATGGAGTTCC AGTCTCAGAG CTCTGTTTCC 
ACTCAGCATG AACAGCAGCA GCAGTCTCCA ATGTTTCATA ATTCTCCGCA ACTTCAGCTT 
GTTCAGGCCT CCACAACCTC TCCAGAGCAA CAGGTTACAT TGTTTATGTC CCCCGTTTCC 
ATGTCAGCCT TGCAGAGCAG CATCAACCAG CAAGACCTTC AGCAAGCCAG TATATACTCA 
CAGTCTATCC AGGGCAGTAC ACCACCTCCT CCCCAGCAGT CTAATATATA CCACAGTTCC 
ACAAGTGGAG CAATGGGCCA AATTCAAGGT CAGCCAACAT CATCACCGCA GGCATCAGGA 
ATGTTTTTGT TCGGGATTCA AAGCAATTGC GGTCAGCTGA TCTCCCAGGC TGCTGCTTCC 
ATACCTGATC AGATAATGGT TATGGCACAA TCAGGTCAAT CGCAAGGAGA TGGTCAATCT 
GTAACAGCAA TTCTATCCCA ACAAATGACA GATAATTCAT CAATGACATC TGAACAGAGC 
ATGGAGAAGA TCAGTGATTT GCTAGTTTCT CTGCAGAACC AGGGGACCAG TTTGTCTGGC 
TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913613.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913670.2 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56881
Clone ID Related Accession (Same CDS sequence)	XM_004913613.4
Accession Version	XM_004913613.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913613.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913670.2
CDS	281..4591
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSQETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSTAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913613.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913613.4

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913614.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913671.2 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa56882
Clone ID Related Accession (Same CDS sequence)	XM_004913614.4
Accession Version	XM_004913614.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913614.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAACA 
ACAACCATTG TATCAGCTCA GCAAAATCTA GAAAATTCAT CAAATGCTAG ACCTTTTTCA 
CCCACTGTAT CCCATCTTCC CACTGATGCT GACCAACAGG CATCAATTCA AGTGCAGACA 
TTCAGCACAG AACCTCTGGC CACCATCCAG ACCCAAGACA TTGCCCAGCC TGGTAGTTTT 
CAGTCAGTGA CATCTCCGGG CCAGGCTAAT GATTCCATAA TGCAGCAGAC TGGACAATTT 
CAAACTATGG ATGGCCAACC CAGGGACACA ATGCAGCCAG AAAACGCCAT TGTCACACTT 
TCACAAATGG CTGAAGTTAC TCCACAAGTG CAGCAGTCCA CAATGCAAGA GCAAGCGCAG 
ACATTACAAC AGCAGATTAC TACAGGCTTA TTCACCTCCA CAAGTAGCGT TAACCAGTTA 
CAGAGTGCTA TTAACCAACT ACAGGCTGGT AGCTTTCAGT CTAGTGTAAG TGGTGGTAAT 
ACAAATGTAG ATTTGGTCCA GCAGGTTCTG GACTCCCAGC AGTTGTCTTG CGTACTTTTT 
TCTGGACAAA GCGGAAATGA AAGCAAGCAG GCCTCAGTTG GACAAGTTCA GGAGCGGATC 
AGTGCTGGCA TATTCCAACC TGTTGCTTCA ATACAAAACA ATGCAAATTC TGCACTGTTT 
TCCACCCCTG AATCTGTGCA TTCCAGACCA GAAAACGTGC TTTCGGGAAG AACTGACAGC 
CTTCTCCAGC AGGCTGAAAA TGCTTTGTCT AATCAACAGC AGCCCATGGA GACCACAGCA 
GCCCTAGTCA TGGAGATGCA GCAAAGTATT CTTCCCTCTG CTGCAACGAT TCAGCCTGAC 
CTTTTCCAGA CACAAAACTC TGGAACATCA AACATTCAGT CACCTGTCTA TCAGCACCAG 
CAAGGTTCTC ATTTAATCCC TGGACTGTCC ACTAATGAAG AGATGCCAAT GCAGTGTGAT 
ATGTTTGCTA GTGCAGCAGT TCCTGGAAGT GAAAGCAGTG CAAACCAGCA GCAGGTATCT 
TCATCTAGTT CAAATATGTT CCAGCCCACA AGCTCTACAG AAAATGATAA TGCTTCTGCA 
CAGGCAGAGC ACATCCAAAG GAATGTGTTT CAATCTATGG TCCAGATGCA ACACAGCGGA 
GAAACCCAGA ATCAAGTCAG TCTTTTTTCA CCAGATGAAG ACATGCTTGG AGTTCAGACA 
AGTGTAAGCC AACAGCAAGG CAGTGGGCTT TTTCCCCCTG GAGACTTAAT GACTCTTCAG 
TCTAGTAACT TCATGCAACA ATCCCCTCAC TCACACACTA CAATTTATCA CTCACAGAAC 
TCTATTGGGG AGAGCCAGAG TATAAACCAG GAAACTCAGG GCTCTCTCTT CCACTCTACC 
AACACTATGG TGCAGCATCA GAACACTGCA TCTCAAGATC AGCTTCAGTC TCCACTTTTC 
CACCCACAGA ACACATTGTC TGTTTTGCAG AGTTCCTCTG TCACTGCAGA GCAGACCTCG 
TCTAACCTCT TCCTGTCTCA AAGCACTATG AGCTCGCTGC AAAGTAATCC AGTGTCTCAA 
GAGAAGCAGC TCTCCTTTTT CACAAGTCAA AATTCACTCC CTCAGCTCCA AGCTGCTCCA 
ACCACAGAAC AGCAAGCAGC CTTTCAACAG CAAGCCCAAC TGTCTCACAT TCAAAACTCA 
ATCATATCCC AAGAACAGCA GCAACAAGTT TCACAAAATA TATATCAGCC ACAGAACCAA 
CAAACAGCAA TTTTCCAGGC AACTCACTCA ATGGTTGGGA TTCAGGGTAG TCCATCTTCT 
CATGAGCAGA GACAAAACAT CCTATTCAGC ACACAAAATT CACTGTCTAA CATGGGATCG 
CCAGAGCAGC AGCAAAGCAT GCTTTTTAAT CCTGGACAGG AGCCAATGTC CACCCAGGAG 
CAGCAGAACC AAGCTCTCTT TCATTCCCAG AACATGGAGT TCCAGTCTCA GAGCTCTGTT 
TCCACTCAGC ATGAACAGCA GCAGCAGTCT CCAATGTTTC ATAATTCTCC GCAACTTCAG 
CTTGTTCAGG CCTCCACAAC CTCTCCAGAG CAACAGGTTA CATTGTTTAT GTCCCCCGTT 
TCCATGTCAG CCTTGCAGAG CAGCATCAAC CAGCAAGACC TTCAGCAAGC CAGTATATAC 
TCACAGTCTA TCCAGGGCAG TACACCACCT CCTCCCCAGC AGTCTAATAT ATACCACAGT 
TCCACAAGTG GAGCAATGGG CCAAATTCAA GGTCAGCCAA CATCATCACC GCAGGCATCA 
GGAATGTTTT TGTTCGGGAT TCAAAGCAAT TGCGGTCAGC TGATCTCCCA GGCTGCTGCT 
TCCATACCTG ATCAGATAAT GGTTATGGCA CAATCAGGTC AATCGCAAGG AGATGGTCAA 
TCTGTAACAG CAATTCTATC CCAACAAATG ACAGATAATT CATCAATGAC ATCTGAACAG 
AGCATGGAGA AGATCAGTGA TTTGCTAGTT TCTCTGCAGA ACCAGGGGAC CAGTTTGTCT 
GGCTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913671.2
CDS	281..4552
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSQETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913614.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913614.4

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAACA 
ACAACCATTG TATCAGCTCA GCAAAATCTA GAAAATTCAT CAAATGCTAG ACCTTTTTCA 
CCCACTGTAT CCCATCTTCC CACTGATGCT GACCAACAGG CATCAATTCA AGTGCAGACA 
TTCAGCACAG AACCTCTGGC CACCATCCAG ACCCAAGACA TTGCCCAGCC TGGTAGTTTT 
CAGTCAGTGA CATCTCCGGG CCAGGCTAAT GATTCCATAA TGCAGCAGAC TGGACAATTT 
CAAACTATGG ATGGCCAACC CAGGGACACA ATGCAGCCAG AAAACGCCAT TGTCACACTT 
TCACAAATGG CTGAAGTTAC TCCACAAGTG CAGCAGTCCA CAATGCAAGA GCAAGCGCAG 
ACATTACAAC AGCAGATTAC TACAGGCTTA TTCACCTCCA CAAGTAGCGT TAACCAGTTA 
CAGAGTGCTA TTAACCAACT ACAGGCTGGT AGCTTTCAGT CTAGTGTAAG TGGTGGTAAT 
ACAAATGTAG ATTTGGTCCA GCAGGTTCTG GACTCCCAGC AGTTGTCTTG CGTACTTTTT 
TCTGGACAAA GCGGAAATGA AAGCAAGCAG GCCTCAGTTG GACAAGTTCA GGAGCGGATC 
AGTGCTGGCA TATTCCAACC TGTTGCTTCA ATACAAAACA ATGCAAATTC TGCACTGTTT 
TCCACCCCTG AATCTGTGCA TTCCAGACCA GAAAACGTGC TTTCGGGAAG AACTGACAGC 
CTTCTCCAGC AGGCTGAAAA TGCTTTGTCT AATCAACAGC AGCCCATGGA GACCACAGCA 
GCCCTAGTCA TGGAGATGCA GCAAAGTATT CTTCCCTCTG CTGCAACGAT TCAGCCTGAC 
CTTTTCCAGA CACAAAACTC TGGAACATCA AACATTCAGT CACCTGTCTA TCAGCACCAG 
CAAGGTTCTC ATTTAATCCC TGGACTGTCC ACTAATGAAG AGATGCCAAT GCAGTGTGAT 
ATGTTTGCTA GTGCAGCAGT TCCTGGAAGT GAAAGCAGTG CAAACCAGCA GCAGGTATCT 
TCATCTAGTT CAAATATGTT CCAGCCCACA AGCTCTACAG AAAATGATAA TGCTTCTGCA 
CAGGCAGAGC ACATCCAAAG GAATGTGTTT CAATCTATGG TCCAGATGCA ACACAGCGGA 
GAAACCCAGA ATCAAGTCAG TCTTTTTTCA CCAGATGAAG ACATGCTTGG AGTTCAGACA 
AGTGTAAGCC AACAGCAAGG CAGTGGGCTT TTTCCCCCTG GAGACTTAAT GACTCTTCAG 
TCTAGTAACT TCATGCAACA ATCCCCTCAC TCACACACTA CAATTTATCA CTCACAGAAC 
TCTATTGGGG AGAGCCAGAG TATAAACCAG GAAACTCAGG GCTCTCTCTT CCACTCTACC 
AACACTATGG TGCAGCATCA GAACACTGCA TCTCAAGATC AGCTTCAGTC TCCACTTTTC 
CACCCACAGA ACACATTGTC TGTTTTGCAG AGTTCCTCTG TCACTGCAGA GCAGACCTCG 
TCTAACCTCT TCCTGTCTCA AAGCACTATG AGCTCGCTGC AAAGTAATCC AGTGTCTCAA 
GAGAAGCAGC TCTCCTTTTT CACAAGTCAA AATTCACTCC CTCAGCTCCA AGCTGCTCCA 
ACCACAGAAC AGCAAGCAGC CTTTCAACAG CAAGCCCAAC TGTCTCACAT TCAAAACTCA 
ATCATATCCC AAGAACAGCA GCAACAAGTT TCACAAAATA TATATCAGCC ACAGAACCAA 
CAAACAGCAA TTTTCCAGGC AACTCACTCA ATGGTTGGGA TTCAGGGTAG TCCATCTTCT 
CATGAGCAGA GACAAAACAT CCTATTCAGC ACACAAAATT CACTGTCTAA CATGGGATCG 
CCAGAGCAGC AGCAAAGCAT GCTTTTTAAT CCTGGACAGG AGCCAATGTC CACCCAGGAG 
CAGCAGAACC AAGCTCTCTT TCATTCCCAG AACATGGAGT TCCAGTCTCA GAGCTCTGTT 
TCCACTCAGC ATGAACAGCA GCAGCAGTCT CCAATGTTTC ATAATTCTCC GCAACTTCAG 
CTTGTTCAGG CCTCCACAAC CTCTCCAGAG CAACAGGTTA CATTGTTTAT GTCCCCCGTT 
TCCATGTCAG CCTTGCAGAG CAGCATCAAC CAGCAAGACC TTCAGCAAGC CAGTATATAC 
TCACAGTCTA TCCAGGGCAG TACACCACCT CCTCCCCAGC AGTCTAATAT ATACCACAGT 
TCCACAAGTG GAGCAATGGG CCAAATTCAA GGTCAGCCAA CATCATCACC GCAGGCATCA 
GGAATGTTTT TGTTCGGGAT TCAAAGCAAT TGCGGTCAGC TGATCTCCCA GGCTGCTGCT 
TCCATACCTG ATCAGATAAT GGTTATGGCA CAATCAGGTC AATCGCAAGG AGATGGTCAA 
TCTGTAACAG CAATTCTATC CCAACAAATG ACAGATAATT CATCAATGAC ATCTGAACAG 
AGCATGGAGA AGATCAGTGA TTTGCTAGTT TCTCTGCAGA ACCAGGGGAC CAGTTTGTCT 
GGCTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913615.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913672.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X4, mRNA.

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CloneID	OXa56883
Clone ID Related Accession (Same CDS sequence)	XM_004913615.4 , XM_004913617.4 , XM_031900792.1 , XM_004913616.4 , XM_018093511.2
Accession Version	XM_004913615.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913615.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913672.1
CDS	459..4688
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913615.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913615.4

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913617.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913674.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X7, mRNA.

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CloneID	OXa56883
Clone ID Related Accession (Same CDS sequence)	XM_004913615.4 , XM_004913617.4 , XM_031900792.1 , XM_004913616.4 , XM_018093511.2
Accession Version	XM_004913617.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913617.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913674.1
CDS	162..4391
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913617.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913617.4

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_031900792.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756652.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X13, mRNA.

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CloneID	OXa56883
Clone ID Related Accession (Same CDS sequence)	XM_004913615.4 , XM_004913617.4 , XM_031900792.1 , XM_004913616.4 , XM_018093511.2
Accession Version	XM_031900792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756652.1
CDS	230..4459
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_031900792.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900792.1

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913616.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913673.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa56883
Clone ID Related Accession (Same CDS sequence)	XM_004913615.4 , XM_004913617.4 , XM_031900792.1 , XM_004913616.4 , XM_018093511.2
Accession Version	XM_004913616.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913616.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913673.1
CDS	546..4775
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913616.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913616.4

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_018093511.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949000.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X6, mRNA.

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CloneID	OXa56883
Clone ID Related Accession (Same CDS sequence)	XM_004913615.4 , XM_004913617.4 , XM_031900792.1 , XM_004913616.4 , XM_018093511.2
Accession Version	XM_018093511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949000.1
CDS	206..4435
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_018093511.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093511.2

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCTAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913618.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913675.2 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X8, mRNA.

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CloneID	OXa56884
Clone ID Related Accession (Same CDS sequence)	XM_004913618.4
Accession Version	XM_004913618.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913618.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAA CAACAACCAT TGTATCAGCT 
CAGCAAAATC TAGAAAATTC ATCAAATGCT AGACCTTTTT CACCCACTGT ATCCCATCTT 
CCCACTGATG CTGACCAACA GGCATCAATT CAAGTGCAGA CATTCAGCAC AGAACCTCTG 
GCCACCATCC AGACCCAAGA CATTGCCCAG CCTGGTAGTT TTCAGTCAGT GACATCTCCG 
GGCCAGGCTA ATGATTCCAT AATGCAGCAG ACTGGACAAT TTCAAACTAT GGATGGCCAA 
CCCAGGGACA CAATGCAGCC AGAAAACGCC ATTGTCACAC TTTCACAAAT GGCTGAAGTT 
ACTCCACAAG TGCAGCAGTC CACAATGCAA GAGCAAGCGC AGACATTACA ACAGCAGATT 
ACTACAGGCT TATTCACCTC CACAAGTAGC GTTAACCAGT TACAGAGTGC TATTAACCAA 
CTACAGGCTG GTAGCTTTCA GTCTAGTGTA AGTGGTGGTA ATACAAATGT AGATTTGGTC 
CAGCAGGTTC TGGACTCCCA GCAGTTGTCT TGCGTACTTT TTTCTGGACA AAGCGGAAAT 
GAAAGCAAGC AGGCCTCAGT TGGACAAGTT CAGGAGCGGA TCAGTGCTGG CATATTCCAA 
CCTGTTGCTT CAATACAAAA CAATGCAAAT TCTGCACTGT TTTCCACCCC TGAATCTGTG 
CATTCCAGAC CAGAAAACGT GCTTTCGGGA AGAACTGACA GCCTTCTCCA GCAGGCTGAA 
AATGCTTTGT CTAATCAACA GCAGCCCATG GAGACCACAG CAGCCCTAGT CATGGAGATG 
CAGCAAAGTA TTCTTCCCTC TGCTGCAACG ATTCAGCCTG ACCTTTTCCA GACACAAAAC 
TCTGGAACAT CAAACATTCA GTCACCTGTC TATCAGCACC AGCAAGGTTC TCATTTAATC 
CCTGGACTGT CCACTAATGA AGAGATGCCA ATGCAGTGTG ATATGTTTGC TAGTGCAGCA 
GTTCCTGGAA GTGAAAGCAG TGCAAACCAG CAGCAGGTAT CTTCATCTAG TTCAAATATG 
TTCCAGCCCA CAAGCTCTAC AGAAAATGAT AATGCTTCTG CACAGGCAGA GCACATCCAA 
AGGAATGTGT TTCAATCTAT GGTCCAGATG CAACACAGCG GAGAAACCCA GAATCAAGTC 
AGTCTTTTTT CACCAGATGA AGACATGCTT GGAGTTCAGA CAAGTGTAAG CCAACAGCAA 
GGCAGTGGGC TTTTTCCCCC TGGAGACTTA ATGACTCTTC AGTCTAGTAA CTTCATGCAA 
CAATCCCCTC ACTCACACAC TACAATTTAT CACTCACAGA ACTCTATTGG GGAGAGCCAG 
AGTATAAACC AGGAAACTCA GGGCTCTCTC TTCCACTCTA CCAACACTAT GGTGCAGCAT 
CAGAACACTG CATCTCAAGA TCAGCTTCAG TCTCCACTTT TCCACCCACA GAACACATTG 
TCTGTTTTGC AGAGTTCCTC TGTCACTGCA GAGCAGACCT CGTCTAACCT CTTCCTGTCT 
CAAAGCACTA TGAGCTCGCT GCAAAGTAAT CCAGTGTCTC AAGAGAAGCA GCTCTCCTTT 
TTCACAAGTC AAAATTCACT CCCTCAGCTC CAAGCTGCTC CAACCACAGA ACAGCAAGCA 
GCCTTTCAAC AGCAAGCCCA ACTGTCTCAC ATTCAAAACT CAATCATATC CCAAGAACAG 
CAGCAACAAG TTTCACAAAA TATATATCAG CCACAGAACC AACAAACAGC AATTTTCCAG 
GCAACTCACT CAATGGTTGG GATTCAGGGT AGTCCATCTT CTCATGAGCA GAGACAAAAC 
ATCCTATTCA GCACACAAAA TTCACTGTCT AACATGGGAT CGCCAGAGCA GCAGCAAAGC 
ATGCTTTTTA ATCCTGGACA GGAGCCAATG TCCACCCAGG AGCAGCAGAA CCAAGCTCTC 
TTTCATTCCC AGAACATGGA GTTCCAGTCT CAGAGCTCTG TTTCCACTCA GCATGAACAG 
CAGCAGCAGT CTCCAATGTT TCATAATTCT CCGCAACTTC AGCTTGTTCA GGCCTCCACA 
ACCTCTCCAG AGCAACAGGT TACATTGTTT ATGTCCCCCG TTTCCATGTC AGCCTTGCAG 
AGCAGCATCA ACCAGCAAGA CCTTCAGCAA GCCAGTATAT ACTCACAGTC TATCCAGGGC 
AGTACACCAC CTCCTCCCCA GCAGTCTAAT ATATACCACA GTTCCACAAG TGGAGCAATG 
GGCCAAATTC AAGGTCAGCC AACATCATCA CCGCAGGCAT CAGGAATGTT TTTGTTCGGG 
ATTCAAAGCA ATTGCGGTCA GCTGATCTCC CAGGCTGCTG CTTCCATACC TGATCAGATA 
ATGGTTATGG CACAATCAGG TCAATCGCAA GGAGATGGTC AATCTGTAAC AGCAATTCTA 
TCCCAACAAA TGACAGATAA TTCATCAATG ACATCTGAAC AGAGCATGGA GAAGATCAGT 
GATTTGCTAG TTTCTCTGCA GAACCAGGGG ACCAGTTTGT CTGGCTCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913675.2
CDS	281..4474
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSQETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSTAPSACNTDQTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913618.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913618.4

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACA AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCTAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAA CAACAACCAT TGTATCAGCT 
CAGCAAAATC TAGAAAATTC ATCAAATGCT AGACCTTTTT CACCCACTGT ATCCCATCTT 
CCCACTGATG CTGACCAACA GGCATCAATT CAAGTGCAGA CATTCAGCAC AGAACCTCTG 
GCCACCATCC AGACCCAAGA CATTGCCCAG CCTGGTAGTT TTCAGTCAGT GACATCTCCG 
GGCCAGGCTA ATGATTCCAT AATGCAGCAG ACTGGACAAT TTCAAACTAT GGATGGCCAA 
CCCAGGGACA CAATGCAGCC AGAAAACGCC ATTGTCACAC TTTCACAAAT GGCTGAAGTT 
ACTCCACAAG TGCAGCAGTC CACAATGCAA GAGCAAGCGC AGACATTACA ACAGCAGATT 
ACTACAGGCT TATTCACCTC CACAAGTAGC GTTAACCAGT TACAGAGTGC TATTAACCAA 
CTACAGGCTG GTAGCTTTCA GTCTAGTGTA AGTGGTGGTA ATACAAATGT AGATTTGGTC 
CAGCAGGTTC TGGACTCCCA GCAGTTGTCT TGCGTACTTT TTTCTGGACA AAGCGGAAAT 
GAAAGCAAGC AGGCCTCAGT TGGACAAGTT CAGGAGCGGA TCAGTGCTGG CATATTCCAA 
CCTGTTGCTT CAATACAAAA CAATGCAAAT TCTGCACTGT TTTCCACCCC TGAATCTGTG 
CATTCCAGAC CAGAAAACGT GCTTTCGGGA AGAACTGACA GCCTTCTCCA GCAGGCTGAA 
AATGCTTTGT CTAATCAACA GCAGCCCATG GAGACCACAG CAGCCCTAGT CATGGAGATG 
CAGCAAAGTA TTCTTCCCTC TGCTGCAACG ATTCAGCCTG ACCTTTTCCA GACACAAAAC 
TCTGGAACAT CAAACATTCA GTCACCTGTC TATCAGCACC AGCAAGGTTC TCATTTAATC 
CCTGGACTGT CCACTAATGA AGAGATGCCA ATGCAGTGTG ATATGTTTGC TAGTGCAGCA 
GTTCCTGGAA GTGAAAGCAG TGCAAACCAG CAGCAGGTAT CTTCATCTAG TTCAAATATG 
TTCCAGCCCA CAAGCTCTAC AGAAAATGAT AATGCTTCTG CACAGGCAGA GCACATCCAA 
AGGAATGTGT TTCAATCTAT GGTCCAGATG CAACACAGCG GAGAAACCCA GAATCAAGTC 
AGTCTTTTTT CACCAGATGA AGACATGCTT GGAGTTCAGA CAAGTGTAAG CCAACAGCAA 
GGCAGTGGGC TTTTTCCCCC TGGAGACTTA ATGACTCTTC AGTCTAGTAA CTTCATGCAA 
CAATCCCCTC ACTCACACAC TACAATTTAT CACTCACAGA ACTCTATTGG GGAGAGCCAG 
AGTATAAACC AGGAAACTCA GGGCTCTCTC TTCCACTCTA CCAACACTAT GGTGCAGCAT 
CAGAACACTG CATCTCAAGA TCAGCTTCAG TCTCCACTTT TCCACCCACA GAACACATTG 
TCTGTTTTGC AGAGTTCCTC TGTCACTGCA GAGCAGACCT CGTCTAACCT CTTCCTGTCT 
CAAAGCACTA TGAGCTCGCT GCAAAGTAAT CCAGTGTCTC AAGAGAAGCA GCTCTCCTTT 
TTCACAAGTC AAAATTCACT CCCTCAGCTC CAAGCTGCTC CAACCACAGA ACAGCAAGCA 
GCCTTTCAAC AGCAAGCCCA ACTGTCTCAC ATTCAAAACT CAATCATATC CCAAGAACAG 
CAGCAACAAG TTTCACAAAA TATATATCAG CCACAGAACC AACAAACAGC AATTTTCCAG 
GCAACTCACT CAATGGTTGG GATTCAGGGT AGTCCATCTT CTCATGAGCA GAGACAAAAC 
ATCCTATTCA GCACACAAAA TTCACTGTCT AACATGGGAT CGCCAGAGCA GCAGCAAAGC 
ATGCTTTTTA ATCCTGGACA GGAGCCAATG TCCACCCAGG AGCAGCAGAA CCAAGCTCTC 
TTTCATTCCC AGAACATGGA GTTCCAGTCT CAGAGCTCTG TTTCCACTCA GCATGAACAG 
CAGCAGCAGT CTCCAATGTT TCATAATTCT CCGCAACTTC AGCTTGTTCA GGCCTCCACA 
ACCTCTCCAG AGCAACAGGT TACATTGTTT ATGTCCCCCG TTTCCATGTC AGCCTTGCAG 
AGCAGCATCA ACCAGCAAGA CCTTCAGCAA GCCAGTATAT ACTCACAGTC TATCCAGGGC 
AGTACACCAC CTCCTCCCCA GCAGTCTAAT ATATACCACA GTTCCACAAG TGGAGCAATG 
GGCCAAATTC AAGGTCAGCC AACATCATCA CCGCAGGCAT CAGGAATGTT TTTGTTCGGG 
ATTCAAAGCA ATTGCGGTCA GCTGATCTCC CAGGCTGCTG CTTCCATACC TGATCAGATA 
ATGGTTATGG CACAATCAGG TCAATCGCAA GGAGATGGTC AATCTGTAAC AGCAATTCTA 
TCCCAACAAA TGACAGATAA TTCATCAATG ACATCTGAAC AGAGCATGGA GAAGATCAGT 
GATTTGCTAG TTTCTCTGCA GAACCAGGGG ACCAGTTTGT CTGGCTCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_018093513.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949002.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X10, mRNA.

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CloneID	OXa56885
Clone ID Related Accession (Same CDS sequence)	XM_018093513.2 , XM_012962322.3 , XM_004913620.4 , XM_012962319.3
Accession Version	XM_018093513.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949002.1
CDS	330..4475
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_018093513.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093513.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962322.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817776.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa56885
Clone ID Related Accession (Same CDS sequence)	XM_018093513.2 , XM_012962322.3 , XM_004913620.4 , XM_012962319.3
Accession Version	XM_012962322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817776.1
CDS	178..4323
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962322.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962322.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913620.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913677.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X12, mRNA.

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CloneID	OXa56885
Clone ID Related Accession (Same CDS sequence)	XM_018093513.2 , XM_012962322.3 , XM_004913620.4 , XM_012962319.3
Accession Version	XM_004913620.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913620.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913677.1
CDS	234..4379
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913620.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913620.4

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962319.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817773.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa56885
Clone ID Related Accession (Same CDS sequence)	XM_018093513.2 , XM_012962322.3 , XM_004913620.4 , XM_012962319.3
Accession Version	XM_012962319.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817773.1
CDS	296..4441
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962319.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962319.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC TAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962323.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817777.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa56886
Clone ID Related Accession (Same CDS sequence)	XM_012962323.3 , XM_004913622.4 , XM_012962324.3 , XM_004913621.4
Accession Version	XM_012962323.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962323.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817777.1
CDS	270..4040
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962323.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962323.3

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913622.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913679.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X15, mRNA.

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CloneID	OXa56886
Clone ID Related Accession (Same CDS sequence)	XM_012962323.3 , XM_004913622.4 , XM_012962324.3 , XM_004913621.4
Accession Version	XM_004913622.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913679.1
CDS	152..3922
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913622.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913622.4

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962324.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817778.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OXa56886
Clone ID Related Accession (Same CDS sequence)	XM_012962323.3 , XM_004913622.4 , XM_012962324.3 , XM_004913621.4
Accession Version	XM_012962324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817778.1
CDS	208..3978
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962324.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962324.3

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913621.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913678.1 Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa56886
Clone ID Related Accession (Same CDS sequence)	XM_012962323.3 , XM_004913622.4 , XM_012962324.3 , XM_004913621.4
Accession Version	XM_004913621.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913621.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913678.1
CDS	91..3861
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913621.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T cells 5 (nfat5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913621.4

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCTAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913622.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913679.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X14, mRNA.

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CloneID	OXa08901
Clone ID Related Accession (Same CDS sequence)	XM_004913622.3 , XM_012962324.2 , XM_004913621.3 , XM_012962323.2
Accession Version	XM_004913622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913679.1
CDS	151..3921
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913622.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913622.3

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962324.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817778.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa08901
Clone ID Related Accession (Same CDS sequence)	XM_004913622.3 , XM_012962324.2 , XM_004913621.3 , XM_012962323.2
Accession Version	XM_012962324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817778.1
CDS	207..3977
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962324.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962324.2

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913621.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913678.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OXa08901
Clone ID Related Accession (Same CDS sequence)	XM_004913622.3 , XM_012962324.2 , XM_004913621.3 , XM_012962323.2
Accession Version	XM_004913621.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913678.1
CDS	91..3861
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913621.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913621.3

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962323.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817777.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X13, mRNA.

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CloneID	OXa08901
Clone ID Related Accession (Same CDS sequence)	XM_004913622.3 , XM_012962324.2 , XM_004913621.3 , XM_012962323.2
Accession Version	XM_012962323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817777.1
CDS	269..4039
Translation	MPHLPPLPQWAVLAAPLPPLPALLFTLPHSPIPKLCNRRVAPLLWGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962323.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962323.2

ORF Insert Sequence:

ATGCCTCATC TGCCTCCTCT TCCTCAATGG GCGGTGCTTG CAGCTCCTTT ACCACCACTT 
CCAGCCCTTC TATTTACTCT ACCTCACTCA CCGATACCAA AGCTATGCAA TCGGAGAGTT 
GCTCCTCTGC TCTGGGGAGC TGCAGAGAAT TCAAAAGGAG GAAAAAAGAG CCTGGTGTTG 
TGTGAGCAGT ATCCAGCTAA AGCTGAGGCA AAGGAACTGA AAATAGTTGT GCAGCCAGAA 
ACACAACATC GCGCCAGATA CCTAACAGAG GGAAGTCGAG GATCTGTAAA AGATCGAACA 
CAACAAGGCT TTCCTACTGT GAAGCTGGAA GGCCATAATG AGTCAGTGGT GCTTCAAGTG 
TTTGTTGGTA ATGACTCTGG CAGAGTAAAG CCACATGGAT TCTACCAAGC CTGTAGGGTA 
ACTGGGAGAA ACACCACTCC TTGCAAAGAA GTGGACATAG AGGGTACAAC AGTTATTGAG 
GTTATGCTTG ACCCGAGCAA TATGATGACA CTAGCAGTTG ATTGTGTTGG GATCCTGAAG 
CTTCGGAATG CAGATGTAGA AGCACGGATA GGGGTTGCTG GCTCTAAGAA AAAGAGCACT 
CGTGCCAGAC TTGTGTTTCG AGTAAACATC ACTCGTTCAG ATGGCTCAAT TTTAACACTT 
CAAACACCTT CTTCACCTAT TCTCTGTACC CAGCCTGCTG GTGTACCTGA AATCCTAAAG 
AAGAGTTTAC ATAGTTGCTC AGTGAAAGGG GAGGAAGAAG TCTTTCTCAT TGGCAAAAAC 
TTTTTAAAAG GAGCAAAAGT TGTATTCCAA GAGAATATCG CAGATGACAA TTCCTGGAAA 
GCTGAAGCTG AAATTGACAT GGAGTTATTT CATCAGAATC ACCTTATAGT GAAGGTTCCT 
TCTTTCCGCG ATCAGCACAT TAGTTCCCCT GTTTCAGTGG TTATGTTTGT GGTGACAAAC 
GCAGGTCGAT CTCATGATGT TCAGCCTTTC ACATACACTC CAGATCAATC CATTCCAGTA 
AATGTTCTTA TAAAGAAAGA GATTCCCAGC CCAACTCAGT CCTGCACTTT TGAAGACACT 
ATGAAAGCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_002937577.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937623.2 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa08895
Clone ID Related Accession (Same CDS sequence)	XM_002937577.4
Accession Version	XM_002937577.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937577.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAGTG 
AGTTTGCTCC CAAGTACTCT GGGTTCTCCT CTTAAATCAA GTGGTTTAAT CAAAAATGAA 
GATAGTGCTC CTATGGAGAT GGCTGCTGAT CAGAGATCCT CTCCTCTATA CAAAACAACA 
ACCATTGTAT CAGCTCAGCA AAATCTAGAA AATTCATCAA ATGCTAGACC TTTTTCACCC 
ACTGTATCCC ATCTTCCCAC TGATGCTGAC CAACAGGCAT CAATTCAAGT GCAGACATTC 
AGCACAGAAC CTCTGGCCAC CATCCAGACC CAAGACATTG CCCAGCCTGG TAGTTTTCAG 
TCAGTGACAT CTCCGGGCCA GGCTAATGAT TCCATAATGC AGCAGACTGG ACAATTTCAA 
ACTATGGATG GCCAACCCAG GGACACAATG CAGCCAGAAA ACGCCATTGT CACACTTTCA 
CAAATGGCTG AAGTTACTCC ACAAGTGCAG CAGTCCACAA TGCAAGAGCA AGCGCAGACA 
TTACAACAGC AGATTACTAC AGGCTTATTC ACCTCCACAA GTAGCGTTAA CCAGTTACAG 
AGTGCTATTA ACCAACTACA GGCTGGTAGC TTTCAGTCTA GTGTAAGTGG TGGTAATACA 
AATGTAGATT TGGTCCAGCA GGTTCTGGAC TCCCAGCAGT TGTCTTGCGT ACTTTTTTCT 
GGACAAAGCG GAAATGAAAG CAAGCAGGCC TCAGTTGGAC AAGTTCAGGA GCGGATCAGT 
GCTGGCATAT TCCAACCTGT TGCTTCAATA CAAAACAATG CAAATTCTGC ACTGTTTTCC 
ACCCCTGAAT CTGTGCATTC CAGACCAGAA AACGTGCTTT CGGGAAGAAC TGACAGCCTT 
CTCCAGCAGG CTGAAAATGC TTTGTCTAAT CAACAGCAGC CCATGGAGAC CACAGCAGCC 
CTAGTCATGG AGATGCAGCA AAGTATTCTT CCCTCTGCTG CAACGATTCA GCCTGACCTT 
TTCCAGACAC AAAACTCTGG AACATCAAAC ATTCAGTCAC CTGTCTATCA GCACCAGCAA 
GGTTCTCATT TAATCCCTGG ACTGTCCACT AATGAAGAGA TGCCAATGCA GTGTGATATG 
TTTGCTAGTG CAGCAGTTCC TGGAAGTGAA AGCAGTGCAA ACCAGCAGCA GGTATCTTCA 
TCTAGTTCAA ATATGTTCCA GCCCACAAGC TCTACAGAAA ATGATAATGC TTCTGCACAG 
GCAGAGCACA TCCAAAGGAA TGTGTTTCAA TCTATGGTCC AGATGCAACA CAGCGGAGAA 
ACCCAGAATC AAGTCAGTCT TTTTTCACCA GATGAAGACA TGCTTGGAGT TCAGACAAGT 
GTAAGCCAAC AGCAAGGCAG TGGGCTTTTT CCCCCTGGAG ACTTAATGAC TCTTCAGTCT 
AGTAACTTCA TGCAACAATC CCCTCACTCA CACACTACAA TTTATCACTC ACAGAACTCT 
ATTGGGGAGA GCCAGAGTAT AAACCAGGAA ACTCAGGGCT CTCTCTTCCA CTCTACCAAC 
ACTATGGTGC AGCATCAGAA CACTGCATCT CAAGATCAGC TTCAGTCTCC ACTTTTCCAC 
CCACAGAACA CATTGTCTGT TTTGCAGAGT TCCTCTGTCA CTGCAGAGCA GACCTCGTCT 
AACCTCTTCC TGTCTCAAAG CACTATGAGC TCGCTGCAAA GTAATCCAGT GTCTCAAGAG 
AAGCAGCTCT CCTTTTTCAC AAGTCAAAAT TCACTCCCTC AGCTCCAAGC TGCTCCAACC 
ACAGAACAGC AAGCAGCCTT TCAACAGCAA GCCCAACTGT CTCACATTCA AAACTCAATC 
ATATCCCAAG AACAGCAGCA ACAAGTTTCA CAAAATATAT ATCAGCCACA GAACCAACAA 
ACAGCAATTT TCCAGGCAAC TCACTCAATG GTTGGGATTC AGGGTAGTCC ATCTTCTCAT 
GAGCAGAGAC AAAACATCCT ATTCAGCACA CAAAATTCAC TGTCTAACAT GGGATCGCCA 
GAGCAGCAGC AAAGCATGCT TTTTAATCCT GGACAGGAGC CAATGTCCAC CCAGGAGCAG 
CAGAACCAAG CTCTCTTTCA TTCCCAGAAC ATGGAGTTCC AGTCTCAGAG CTCTGTTTCC 
ACTCAGCATG AACAGCAGCA GCAGTCTCCA ATGTTTCATA ATTCTCCGCA ACTTCAGCTT 
GTTCAGGCCT CCACAACCTC TCCAGAGCAA CAGGTTACAT TGTTTATGTC CCCCGTTTCC 
ATGTCAGCCT TGCAGAGCAG CATCAACCAG CAAGACCTTC AGCAAGCCAG TATATACTCA 
CAGTCTATCC AGGGCAGTAC ACCACCTCCT CCCCAGCAGT CTAATATATA CCACAGTTCC 
ACAAGTGGAG CAATGGGCCA AATTCAAGGT CAGCCAACAT CATCACCGCA GGCATCAGGA 
ATGTTTTTGT TCGGGATTCA AAGCAATTGC GGTCAGCTGA TCTCCCAGGC TGCTGCTTCC 
ATACCTGATC AGATAATGGT TATGGCACAA TCAGGTCAAT CGCAAGGAGA TGGTCAATCT 
GTAACAGCAA TTCTATCCCA ACAAATGACA GATAATTCAT CAATGACATC TGAACAGAGC 
ATGGAGAAGA TCAGTGATTT GCTAGTTTCT CTGCAGAACC AGGGGACCAG TTTGTCTGGC 
TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937623.2
CDS	283..4671
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSRETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_002937577.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937577.4

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAGTG 
AGTTTGCTCC CAAGTACTCT GGGTTCTCCT CTTAAATCAA GTGGTTTAAT CAAAAATGAA 
GATAGTGCTC CTATGGAGAT GGCTGCTGAT CAGAGATCCT CTCCTCTATA CAAAACAACA 
ACCATTGTAT CAGCTCAGCA AAATCTAGAA AATTCATCAA ATGCTAGACC TTTTTCACCC 
ACTGTATCCC ATCTTCCCAC TGATGCTGAC CAACAGGCAT CAATTCAAGT GCAGACATTC 
AGCACAGAAC CTCTGGCCAC CATCCAGACC CAAGACATTG CCCAGCCTGG TAGTTTTCAG 
TCAGTGACAT CTCCGGGCCA GGCTAATGAT TCCATAATGC AGCAGACTGG ACAATTTCAA 
ACTATGGATG GCCAACCCAG GGACACAATG CAGCCAGAAA ACGCCATTGT CACACTTTCA 
CAAATGGCTG AAGTTACTCC ACAAGTGCAG CAGTCCACAA TGCAAGAGCA AGCGCAGACA 
TTACAACAGC AGATTACTAC AGGCTTATTC ACCTCCACAA GTAGCGTTAA CCAGTTACAG 
AGTGCTATTA ACCAACTACA GGCTGGTAGC TTTCAGTCTA GTGTAAGTGG TGGTAATACA 
AATGTAGATT TGGTCCAGCA GGTTCTGGAC TCCCAGCAGT TGTCTTGCGT ACTTTTTTCT 
GGACAAAGCG GAAATGAAAG CAAGCAGGCC TCAGTTGGAC AAGTTCAGGA GCGGATCAGT 
GCTGGCATAT TCCAACCTGT TGCTTCAATA CAAAACAATG CAAATTCTGC ACTGTTTTCC 
ACCCCTGAAT CTGTGCATTC CAGACCAGAA AACGTGCTTT CGGGAAGAAC TGACAGCCTT 
CTCCAGCAGG CTGAAAATGC TTTGTCTAAT CAACAGCAGC CCATGGAGAC CACAGCAGCC 
CTAGTCATGG AGATGCAGCA AAGTATTCTT CCCTCTGCTG CAACGATTCA GCCTGACCTT 
TTCCAGACAC AAAACTCTGG AACATCAAAC ATTCAGTCAC CTGTCTATCA GCACCAGCAA 
GGTTCTCATT TAATCCCTGG ACTGTCCACT AATGAAGAGA TGCCAATGCA GTGTGATATG 
TTTGCTAGTG CAGCAGTTCC TGGAAGTGAA AGCAGTGCAA ACCAGCAGCA GGTATCTTCA 
TCTAGTTCAA ATATGTTCCA GCCCACAAGC TCTACAGAAA ATGATAATGC TTCTGCACAG 
GCAGAGCACA TCCAAAGGAA TGTGTTTCAA TCTATGGTCC AGATGCAACA CAGCGGAGAA 
ACCCAGAATC AAGTCAGTCT TTTTTCACCA GATGAAGACA TGCTTGGAGT TCAGACAAGT 
GTAAGCCAAC AGCAAGGCAG TGGGCTTTTT CCCCCTGGAG ACTTAATGAC TCTTCAGTCT 
AGTAACTTCA TGCAACAATC CCCTCACTCA CACACTACAA TTTATCACTC ACAGAACTCT 
ATTGGGGAGA GCCAGAGTAT AAACCAGGAA ACTCAGGGCT CTCTCTTCCA CTCTACCAAC 
ACTATGGTGC AGCATCAGAA CACTGCATCT CAAGATCAGC TTCAGTCTCC ACTTTTCCAC 
CCACAGAACA CATTGTCTGT TTTGCAGAGT TCCTCTGTCA CTGCAGAGCA GACCTCGTCT 
AACCTCTTCC TGTCTCAAAG CACTATGAGC TCGCTGCAAA GTAATCCAGT GTCTCAAGAG 
AAGCAGCTCT CCTTTTTCAC AAGTCAAAAT TCACTCCCTC AGCTCCAAGC TGCTCCAACC 
ACAGAACAGC AAGCAGCCTT TCAACAGCAA GCCCAACTGT CTCACATTCA AAACTCAATC 
ATATCCCAAG AACAGCAGCA ACAAGTTTCA CAAAATATAT ATCAGCCACA GAACCAACAA 
ACAGCAATTT TCCAGGCAAC TCACTCAATG GTTGGGATTC AGGGTAGTCC ATCTTCTCAT 
GAGCAGAGAC AAAACATCCT ATTCAGCACA CAAAATTCAC TGTCTAACAT GGGATCGCCA 
GAGCAGCAGC AAAGCATGCT TTTTAATCCT GGACAGGAGC CAATGTCCAC CCAGGAGCAG 
CAGAACCAAG CTCTCTTTCA TTCCCAGAAC ATGGAGTTCC AGTCTCAGAG CTCTGTTTCC 
ACTCAGCATG AACAGCAGCA GCAGTCTCCA ATGTTTCATA ATTCTCCGCA ACTTCAGCTT 
GTTCAGGCCT CCACAACCTC TCCAGAGCAA CAGGTTACAT TGTTTATGTC CCCCGTTTCC 
ATGTCAGCCT TGCAGAGCAG CATCAACCAG CAAGACCTTC AGCAAGCCAG TATATACTCA 
CAGTCTATCC AGGGCAGTAC ACCACCTCCT CCCCAGCAGT CTAATATATA CCACAGTTCC 
ACAAGTGGAG CAATGGGCCA AATTCAAGGT CAGCCAACAT CATCACCGCA GGCATCAGGA 
ATGTTTTTGT TCGGGATTCA AAGCAATTGC GGTCAGCTGA TCTCCCAGGC TGCTGCTTCC 
ATACCTGATC AGATAATGGT TATGGCACAA TCAGGTCAAT CGCAAGGAGA TGGTCAATCT 
GTAACAGCAA TTCTATCCCA ACAAATGACA GATAATTCAT CAATGACATC TGAACAGAGC 
ATGGAGAAGA TCAGTGATTT GCTAGTTTCT CTGCAGAACC AGGGGACCAG TTTGTCTGGC 
TCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913613.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913670.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa08896
Clone ID Related Accession (Same CDS sequence)	XM_004913613.3
Accession Version	XM_004913613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913670.1
CDS	283..4593
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSRETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSTAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913613.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913613.3

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAG TGAGTTTGCT CCCAAGTACT 
CTGGGTTCTC CTCTTAAATC AAGTGGTTTA ATCAAAAATG AAGATAGTGC TCCTATGGAG 
ATGGCTGCTG ATCAGAGATC CTCTCCTCTA TACAAAACAA CAACCATTGT ATCAGCTCAG 
CAAAATCTAG AAAATTCATC AAATGCTAGA CCTTTTTCAC CCACTGTATC CCATCTTCCC 
ACTGATGCTG ACCAACAGGC ATCAATTCAA GTGCAGACAT TCAGCACAGA ACCTCTGGCC 
ACCATCCAGA CCCAAGACAT TGCCCAGCCT GGTAGTTTTC AGTCAGTGAC ATCTCCGGGC 
CAGGCTAATG ATTCCATAAT GCAGCAGACT GGACAATTTC AAACTATGGA TGGCCAACCC 
AGGGACACAA TGCAGCCAGA AAACGCCATT GTCACACTTT CACAAATGGC TGAAGTTACT 
CCACAAGTGC AGCAGTCCAC AATGCAAGAG CAAGCGCAGA CATTACAACA GCAGATTACT 
ACAGGCTTAT TCACCTCCAC AAGTAGCGTT AACCAGTTAC AGAGTGCTAT TAACCAACTA 
CAGGCTGGTA GCTTTCAGTC TAGTGTAAGT GGTGGTAATA CAAATGTAGA TTTGGTCCAG 
CAGGTTCTGG ACTCCCAGCA GTTGTCTTGC GTACTTTTTT CTGGACAAAG CGGAAATGAA 
AGCAAGCAGG CCTCAGTTGG ACAAGTTCAG GAGCGGATCA GTGCTGGCAT ATTCCAACCT 
GTTGCTTCAA TACAAAACAA TGCAAATTCT GCACTGTTTT CCACCCCTGA ATCTGTGCAT 
TCCAGACCAG AAAACGTGCT TTCGGGAAGA ACTGACAGCC TTCTCCAGCA GGCTGAAAAT 
GCTTTGTCTA ATCAACAGCA GCCCATGGAG ACCACAGCAG CCCTAGTCAT GGAGATGCAG 
CAAAGTATTC TTCCCTCTGC TGCAACGATT CAGCCTGACC TTTTCCAGAC ACAAAACTCT 
GGAACATCAA ACATTCAGTC ACCTGTCTAT CAGCACCAGC AAGGTTCTCA TTTAATCCCT 
GGACTGTCCA CTAATGAAGA GATGCCAATG CAGTGTGATA TGTTTGCTAG TGCAGCAGTT 
CCTGGAAGTG AAAGCAGTGC AAACCAGCAG CAGGTATCTT CATCTAGTTC AAATATGTTC 
CAGCCCACAA GCTCTACAGA AAATGATAAT GCTTCTGCAC AGGCAGAGCA CATCCAAAGG 
AATGTGTTTC AATCTATGGT CCAGATGCAA CACAGCGGAG AAACCCAGAA TCAAGTCAGT 
CTTTTTTCAC CAGATGAAGA CATGCTTGGA GTTCAGACAA GTGTAAGCCA ACAGCAAGGC 
AGTGGGCTTT TTCCCCCTGG AGACTTAATG ACTCTTCAGT CTAGTAACTT CATGCAACAA 
TCCCCTCACT CACACACTAC AATTTATCAC TCACAGAACT CTATTGGGGA GAGCCAGAGT 
ATAAACCAGG AAACTCAGGG CTCTCTCTTC CACTCTACCA ACACTATGGT GCAGCATCAG 
AACACTGCAT CTCAAGATCA GCTTCAGTCT CCACTTTTCC ACCCACAGAA CACATTGTCT 
GTTTTGCAGA GTTCCTCTGT CACTGCAGAG CAGACCTCGT CTAACCTCTT CCTGTCTCAA 
AGCACTATGA GCTCGCTGCA AAGTAATCCA GTGTCTCAAG AGAAGCAGCT CTCCTTTTTC 
ACAAGTCAAA ATTCACTCCC TCAGCTCCAA GCTGCTCCAA CCACAGAACA GCAAGCAGCC 
TTTCAACAGC AAGCCCAACT GTCTCACATT CAAAACTCAA TCATATCCCA AGAACAGCAG 
CAACAAGTTT CACAAAATAT ATATCAGCCA CAGAACCAAC AAACAGCAAT TTTCCAGGCA 
ACTCACTCAA TGGTTGGGAT TCAGGGTAGT CCATCTTCTC ATGAGCAGAG ACAAAACATC 
CTATTCAGCA CACAAAATTC ACTGTCTAAC ATGGGATCGC CAGAGCAGCA GCAAAGCATG 
CTTTTTAATC CTGGACAGGA GCCAATGTCC ACCCAGGAGC AGCAGAACCA AGCTCTCTTT 
CATTCCCAGA ACATGGAGTT CCAGTCTCAG AGCTCTGTTT CCACTCAGCA TGAACAGCAG 
CAGCAGTCTC CAATGTTTCA TAATTCTCCG CAACTTCAGC TTGTTCAGGC CTCCACAACC 
TCTCCAGAGC AACAGGTTAC ATTGTTTATG TCCCCCGTTT CCATGTCAGC CTTGCAGAGC 
AGCATCAACC AGCAAGACCT TCAGCAAGCC AGTATATACT CACAGTCTAT CCAGGGCAGT 
ACACCACCTC CTCCCCAGCA GTCTAATATA TACCACAGTT CCACAAGTGG AGCAATGGGC 
CAAATTCAAG GTCAGCCAAC ATCATCACCG CAGGCATCAG GAATGTTTTT GTTCGGGATT 
CAAAGCAATT GCGGTCAGCT GATCTCCCAG GCTGCTGCTT CCATACCTGA TCAGATAATG 
GTTATGGCAC AATCAGGTCA ATCGCAAGGA GATGGTCAAT CTGTAACAGC AATTCTATCC 
CAACAAATGA CAGATAATTC ATCAATGACA TCTGAACAGA GCATGGAGAA GATCAGTGAT 
TTGCTAGTTT CTCTGCAGAA CCAGGGGACC AGTTTGTCTG GCTCTTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913614.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913671.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X3, mRNA.

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CloneID	OXa08897
Clone ID Related Accession (Same CDS sequence)	XM_004913614.3
Accession Version	XM_004913614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAACA 
ACAACCATTG TATCAGCTCA GCAAAATCTA GAAAATTCAT CAAATGCTAG ACCTTTTTCA 
CCCACTGTAT CCCATCTTCC CACTGATGCT GACCAACAGG CATCAATTCA AGTGCAGACA 
TTCAGCACAG AACCTCTGGC CACCATCCAG ACCCAAGACA TTGCCCAGCC TGGTAGTTTT 
CAGTCAGTGA CATCTCCGGG CCAGGCTAAT GATTCCATAA TGCAGCAGAC TGGACAATTT 
CAAACTATGG ATGGCCAACC CAGGGACACA ATGCAGCCAG AAAACGCCAT TGTCACACTT 
TCACAAATGG CTGAAGTTAC TCCACAAGTG CAGCAGTCCA CAATGCAAGA GCAAGCGCAG 
ACATTACAAC AGCAGATTAC TACAGGCTTA TTCACCTCCA CAAGTAGCGT TAACCAGTTA 
CAGAGTGCTA TTAACCAACT ACAGGCTGGT AGCTTTCAGT CTAGTGTAAG TGGTGGTAAT 
ACAAATGTAG ATTTGGTCCA GCAGGTTCTG GACTCCCAGC AGTTGTCTTG CGTACTTTTT 
TCTGGACAAA GCGGAAATGA AAGCAAGCAG GCCTCAGTTG GACAAGTTCA GGAGCGGATC 
AGTGCTGGCA TATTCCAACC TGTTGCTTCA ATACAAAACA ATGCAAATTC TGCACTGTTT 
TCCACCCCTG AATCTGTGCA TTCCAGACCA GAAAACGTGC TTTCGGGAAG AACTGACAGC 
CTTCTCCAGC AGGCTGAAAA TGCTTTGTCT AATCAACAGC AGCCCATGGA GACCACAGCA 
GCCCTAGTCA TGGAGATGCA GCAAAGTATT CTTCCCTCTG CTGCAACGAT TCAGCCTGAC 
CTTTTCCAGA CACAAAACTC TGGAACATCA AACATTCAGT CACCTGTCTA TCAGCACCAG 
CAAGGTTCTC ATTTAATCCC TGGACTGTCC ACTAATGAAG AGATGCCAAT GCAGTGTGAT 
ATGTTTGCTA GTGCAGCAGT TCCTGGAAGT GAAAGCAGTG CAAACCAGCA GCAGGTATCT 
TCATCTAGTT CAAATATGTT CCAGCCCACA AGCTCTACAG AAAATGATAA TGCTTCTGCA 
CAGGCAGAGC ACATCCAAAG GAATGTGTTT CAATCTATGG TCCAGATGCA ACACAGCGGA 
GAAACCCAGA ATCAAGTCAG TCTTTTTTCA CCAGATGAAG ACATGCTTGG AGTTCAGACA 
AGTGTAAGCC AACAGCAAGG CAGTGGGCTT TTTCCCCCTG GAGACTTAAT GACTCTTCAG 
TCTAGTAACT TCATGCAACA ATCCCCTCAC TCACACACTA CAATTTATCA CTCACAGAAC 
TCTATTGGGG AGAGCCAGAG TATAAACCAG GAAACTCAGG GCTCTCTCTT CCACTCTACC 
AACACTATGG TGCAGCATCA GAACACTGCA TCTCAAGATC AGCTTCAGTC TCCACTTTTC 
CACCCACAGA ACACATTGTC TGTTTTGCAG AGTTCCTCTG TCACTGCAGA GCAGACCTCG 
TCTAACCTCT TCCTGTCTCA AAGCACTATG AGCTCGCTGC AAAGTAATCC AGTGTCTCAA 
GAGAAGCAGC TCTCCTTTTT CACAAGTCAA AATTCACTCC CTCAGCTCCA AGCTGCTCCA 
ACCACAGAAC AGCAAGCAGC CTTTCAACAG CAAGCCCAAC TGTCTCACAT TCAAAACTCA 
ATCATATCCC AAGAACAGCA GCAACAAGTT TCACAAAATA TATATCAGCC ACAGAACCAA 
CAAACAGCAA TTTTCCAGGC AACTCACTCA ATGGTTGGGA TTCAGGGTAG TCCATCTTCT 
CATGAGCAGA GACAAAACAT CCTATTCAGC ACACAAAATT CACTGTCTAA CATGGGATCG 
CCAGAGCAGC AGCAAAGCAT GCTTTTTAAT CCTGGACAGG AGCCAATGTC CACCCAGGAG 
CAGCAGAACC AAGCTCTCTT TCATTCCCAG AACATGGAGT TCCAGTCTCA GAGCTCTGTT 
TCCACTCAGC ATGAACAGCA GCAGCAGTCT CCAATGTTTC ATAATTCTCC GCAACTTCAG 
CTTGTTCAGG CCTCCACAAC CTCTCCAGAG CAACAGGTTA CATTGTTTAT GTCCCCCGTT 
TCCATGTCAG CCTTGCAGAG CAGCATCAAC CAGCAAGACC TTCAGCAAGC CAGTATATAC 
TCACAGTCTA TCCAGGGCAG TACACCACCT CCTCCCCAGC AGTCTAATAT ATACCACAGT 
TCCACAAGTG GAGCAATGGG CCAAATTCAA GGTCAGCCAA CATCATCACC GCAGGCATCA 
GGAATGTTTT TGTTCGGGAT TCAAAGCAAT TGCGGTCAGC TGATCTCCCA GGCTGCTGCT 
TCCATACCTG ATCAGATAAT GGTTATGGCA CAATCAGGTC AATCGCAAGG AGATGGTCAA 
TCTGTAACAG CAATTCTATC CCAACAAATG ACAGATAATT CATCAATGAC ATCTGAACAG 
AGCATGGAGA AGATCAGTGA TTTGCTAGTT TCTCTGCAGA ACCAGGGGAC CAGTTTGTCT 
GGCTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913671.1
CDS	282..4553
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSRETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913614.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913614.3

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCCA TTCCAGTAAA TGTTCTTATA AAGAAAGAGA TTCCCAGCCC AACTCAGTCC 
TGCACTTTTG AAGACACTAT GAAAGCAACA GCACCATCTG CCTGCAACAC TGATCAAACA 
ACAACCATTG TATCAGCTCA GCAAAATCTA GAAAATTCAT CAAATGCTAG ACCTTTTTCA 
CCCACTGTAT CCCATCTTCC CACTGATGCT GACCAACAGG CATCAATTCA AGTGCAGACA 
TTCAGCACAG AACCTCTGGC CACCATCCAG ACCCAAGACA TTGCCCAGCC TGGTAGTTTT 
CAGTCAGTGA CATCTCCGGG CCAGGCTAAT GATTCCATAA TGCAGCAGAC TGGACAATTT 
CAAACTATGG ATGGCCAACC CAGGGACACA ATGCAGCCAG AAAACGCCAT TGTCACACTT 
TCACAAATGG CTGAAGTTAC TCCACAAGTG CAGCAGTCCA CAATGCAAGA GCAAGCGCAG 
ACATTACAAC AGCAGATTAC TACAGGCTTA TTCACCTCCA CAAGTAGCGT TAACCAGTTA 
CAGAGTGCTA TTAACCAACT ACAGGCTGGT AGCTTTCAGT CTAGTGTAAG TGGTGGTAAT 
ACAAATGTAG ATTTGGTCCA GCAGGTTCTG GACTCCCAGC AGTTGTCTTG CGTACTTTTT 
TCTGGACAAA GCGGAAATGA AAGCAAGCAG GCCTCAGTTG GACAAGTTCA GGAGCGGATC 
AGTGCTGGCA TATTCCAACC TGTTGCTTCA ATACAAAACA ATGCAAATTC TGCACTGTTT 
TCCACCCCTG AATCTGTGCA TTCCAGACCA GAAAACGTGC TTTCGGGAAG AACTGACAGC 
CTTCTCCAGC AGGCTGAAAA TGCTTTGTCT AATCAACAGC AGCCCATGGA GACCACAGCA 
GCCCTAGTCA TGGAGATGCA GCAAAGTATT CTTCCCTCTG CTGCAACGAT TCAGCCTGAC 
CTTTTCCAGA CACAAAACTC TGGAACATCA AACATTCAGT CACCTGTCTA TCAGCACCAG 
CAAGGTTCTC ATTTAATCCC TGGACTGTCC ACTAATGAAG AGATGCCAAT GCAGTGTGAT 
ATGTTTGCTA GTGCAGCAGT TCCTGGAAGT GAAAGCAGTG CAAACCAGCA GCAGGTATCT 
TCATCTAGTT CAAATATGTT CCAGCCCACA AGCTCTACAG AAAATGATAA TGCTTCTGCA 
CAGGCAGAGC ACATCCAAAG GAATGTGTTT CAATCTATGG TCCAGATGCA ACACAGCGGA 
GAAACCCAGA ATCAAGTCAG TCTTTTTTCA CCAGATGAAG ACATGCTTGG AGTTCAGACA 
AGTGTAAGCC AACAGCAAGG CAGTGGGCTT TTTCCCCCTG GAGACTTAAT GACTCTTCAG 
TCTAGTAACT TCATGCAACA ATCCCCTCAC TCACACACTA CAATTTATCA CTCACAGAAC 
TCTATTGGGG AGAGCCAGAG TATAAACCAG GAAACTCAGG GCTCTCTCTT CCACTCTACC 
AACACTATGG TGCAGCATCA GAACACTGCA TCTCAAGATC AGCTTCAGTC TCCACTTTTC 
CACCCACAGA ACACATTGTC TGTTTTGCAG AGTTCCTCTG TCACTGCAGA GCAGACCTCG 
TCTAACCTCT TCCTGTCTCA AAGCACTATG AGCTCGCTGC AAAGTAATCC AGTGTCTCAA 
GAGAAGCAGC TCTCCTTTTT CACAAGTCAA AATTCACTCC CTCAGCTCCA AGCTGCTCCA 
ACCACAGAAC AGCAAGCAGC CTTTCAACAG CAAGCCCAAC TGTCTCACAT TCAAAACTCA 
ATCATATCCC AAGAACAGCA GCAACAAGTT TCACAAAATA TATATCAGCC ACAGAACCAA 
CAAACAGCAA TTTTCCAGGC AACTCACTCA ATGGTTGGGA TTCAGGGTAG TCCATCTTCT 
CATGAGCAGA GACAAAACAT CCTATTCAGC ACACAAAATT CACTGTCTAA CATGGGATCG 
CCAGAGCAGC AGCAAAGCAT GCTTTTTAAT CCTGGACAGG AGCCAATGTC CACCCAGGAG 
CAGCAGAACC AAGCTCTCTT TCATTCCCAG AACATGGAGT TCCAGTCTCA GAGCTCTGTT 
TCCACTCAGC ATGAACAGCA GCAGCAGTCT CCAATGTTTC ATAATTCTCC GCAACTTCAG 
CTTGTTCAGG CCTCCACAAC CTCTCCAGAG CAACAGGTTA CATTGTTTAT GTCCCCCGTT 
TCCATGTCAG CCTTGCAGAG CAGCATCAAC CAGCAAGACC TTCAGCAAGC CAGTATATAC 
TCACAGTCTA TCCAGGGCAG TACACCACCT CCTCCCCAGC AGTCTAATAT ATACCACAGT 
TCCACAAGTG GAGCAATGGG CCAAATTCAA GGTCAGCCAA CATCATCACC GCAGGCATCA 
GGAATGTTTT TGTTCGGGAT TCAAAGCAAT TGCGGTCAGC TGATCTCCCA GGCTGCTGCT 
TCCATACCTG ATCAGATAAT GGTTATGGCA CAATCAGGTC AATCGCAAGG AGATGGTCAA 
TCTGTAACAG CAATTCTATC CCAACAAATG ACAGATAATT CATCAATGAC ATCTGAACAG 
AGCATGGAGA AGATCAGTGA TTTGCTAGTT TCTCTGCAGA ACCAGGGGAC CAGTTTGTCT 
GGCTCTTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_018093511.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949000.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X7, mRNA.

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CloneID	OXa08898
Clone ID Related Accession (Same CDS sequence)	XM_018093511.1 , XM_004913615.3 , XM_004913616.3 , XM_004913617.3
Accession Version	XM_018093511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949000.1
CDS	206..4435
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_018093511.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093511.1

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913615.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913672.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa08898
Clone ID Related Accession (Same CDS sequence)	XM_018093511.1 , XM_004913615.3 , XM_004913616.3 , XM_004913617.3
Accession Version	XM_004913615.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913615.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913672.1
CDS	473..4702
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913615.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913615.3

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913616.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913673.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa08898
Clone ID Related Accession (Same CDS sequence)	XM_018093511.1 , XM_004913615.3 , XM_004913616.3 , XM_004913617.3
Accession Version	XM_004913616.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913673.1
CDS	560..4789
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913616.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913616.3

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913617.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913674.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X6, mRNA.

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CloneID	OXa08898
Clone ID Related Accession (Same CDS sequence)	XM_018093511.1 , XM_004913615.3 , XM_004913616.3 , XM_004913617.3
Accession Version	XM_004913617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913674.1
CDS	168..4397
Translation	MSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913617.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913617.3

ORF Insert Sequence:

ATGAGTCAGA CAAGCGGAGG TGAGGCAGGC TCACCGCCCC CGGCTGTTAT AGCTGCTGAT 
GCCTCATCTG CCTCCTCTTC CTCAATGGGC GGTGCTTGCA GCTCCTTTAC CACCACTTCC 
AGCCCTTCTA TTTACTCTAC CTCACTCACC GATACCAAAG CTATGCAATC GGAGAGTTGC 
TCCTCTGCTC TGGGGGTAAG TAAACCAGGG GTAAGTGAAC AGCTCTTAGC CAGTAATGCA 
GGCCAGCAGC AGACCGGGCC ACTAAGATGG ACGGTCCTTA ACATTTCGCC ACCACCCGAA 
GACTTGCTCG ATAACAGTCA GATGTCATGC CAAGATGAAG GGGGTGGACT GGAGTCGGAG 
CAGAGTTGCA GTATGTGGAC TGAAGATTCC ACCTCCAATT TTAGCAACAT GAGCACCCAT 
TCTTACAATG ATAACACCGA GGTGCCTCGT AAGTCACGGA AAAGAAACCC AAAGCAAAGG 
CCTGGAGCGA AACGGCAGGA TTCCAACATG GATGTTTTTG ATGCTGATAG CGCCAAAGCT 
CCCCATTATG TGCTTTCCCA GCTCAACTCG GACAGCAAAG GCCATACTGG AAATGGAGCT 
GCAGAGAATT CAAAAGGAGG AAAAAAGAGC CTGGTGTTGT GTGAGCAGTA TCCAGCTAAA 
GCTGAGGCAA AGGAACTGAA AATAGTTGTG CAGCCAGAAA CACAACATCG CGCCAGATAC 
CTAACAGAGG GAAGTCGAGG ATCTGTAAAA GATCGAACAC AACAAGGCTT TCCTACTGTG 
AAGCTGGAAG GCCATAATGA GTCAGTGGTG CTTCAAGTGT TTGTTGGTAA TGACTCTGGC 
AGAGTAAAGC CACATGGATT CTACCAAGCC TGTAGGGTAA CTGGGAGAAA CACCACTCCT 
TGCAAAGAAG TGGACATAGA GGGTACAACA GTTATTGAGG TTATGCTTGA CCCGAGCAAT 
ATGATGACAC TAGCAGTTGA TTGTGTTGGG ATCCTGAAGC TTCGGAATGC AGATGTAGAA 
GCACGGATAG GGGTTGCTGG CTCTAAGAAA AAGAGCACTC GTGCCAGACT TGTGTTTCGA 
GTAAACATCA CTCGTTCAGA TGGCTCAATT TTAACACTTC AAACACCTTC TTCACCTATT 
CTCTGTACCC AGCCTGCTGG TGTACCTGAA ATCCTAAAGA AGAGTTTACA TAGTTGCTCA 
GTGAAAGGGG AGGAAGAAGT CTTTCTCATT GGCAAAAACT TTTTAAAAGG AGCAAAAGTT 
GTATTCCAAG AGAATATCGC AGATGACAAT TCCTGGAAAG CTGAAGCTGA AATTGACATG 
GAGTTATTTC ATCAGAATCA CCTTATAGTG AAGGTTCCTT CTTTCCGCGA TCAGCACATT 
AGTTCCCCTG TTTCAGTGGT TATGTTTGTG GTGACAAACG CAGGTCGATC TCATGATGTT 
CAGCCTTTCA CATACACTCC AGATCAATCC ATTCCAGTAA ATGTTCTTAT AAAGAAAGAG 
ATTCCCAGCC CAACTCAGTC CTGCACTTTT GAAGACACTA TGAAAGCAAC AGCACCATCT 
GCCTGCAACA CTGATCAAGT GAGTTTGCTC CCAAGTACTC TGGGTTCTCC TCTTAAATCA 
AGTGGTTTAA TCAAAAATGA AGATAGTGCT CCTATGGAGA TGGCTGCTGA TCAGAGATCC 
TCTCCTCTAT ACAAAACAAC AACCATTGTA TCAGCTCAGC AAAATCTAGA AAATTCATCA 
AATGCTAGAC CTTTTTCACC CACTGTATCC CATCTTCCCA CTGATGCTGA CCAACAGGCA 
TCAATTCAAG TGCAGACATT CAGCACAGAA CCTCTGGCCA CCATCCAGAC CCAAGACATT 
GCCCAGCCTG GTAGTTTTCA GTCAGTGACA TCTCCGGGCC AGGCTAATGA TTCCATAATG 
CAGCAGACTG GACAATTTCA AACTATGGAT GGCCAACCCA GGGACACAAT GCAGCCAGAA 
AACGCCATTG TCACACTTTC ACAAATGGCT GAAGTTACTC CACAAGTGCA GCAGTCCACA 
ATGCAAGAGC AAGCGCAGAC ATTACAACAG CAGATTACTA CAGGCTTATT CACCTCCACA 
AGTAGCGTTA ACCAGTTACA GAGTGCTATT AACCAACTAC AGGCTGGTAG CTTTCAGTCT 
AGTGTAAGTG GTGGTAATAC AAATGTAGAT TTGGTCCAGC AGGTTCTGGA CTCCCAGCAG 
TTGTCTTGCG TACTTTTTTC TGGACAAAGC GGAAATGAAA GCAAGCAGGC CTCAGTTGGA 
CAAGTTCAGG AGCGGATCAG TGCTGGCATA TTCCAACCTG TTGCTTCAAT ACAAAACAAT 
GCAAATTCTG CACTGTTTTC CACCCCTGAA TCTGTGCATT CCAGACCAGA AAACGTGCTT 
TCGGGAAGAA CTGACAGCCT TCTCCAGCAG GCTGAAAATG CTTTGTCTAA TCAACAGCAG 
CCCATGGAGA CCACAGCAGC CCTAGTCATG GAGATGCAGC AAAGTATTCT TCCCTCTGCT 
GCAACGATTC AGCCTGACCT TTTCCAGACA CAAAACTCTG GAACATCAAA CATTCAGTCA 
CCTGTCTATC AGCACCAGCA AGGTTCTCAT TTAATCCCTG GACTGTCCAC TAATGAAGAG 
ATGCCAATGC AGTGTGATAT GTTTGCTAGT GCAGCAGTTC CTGGAAGTGA AAGCAGTGCA 
AACCAGCAGC AGGTATCTTC ATCTAGTTCA AATATGTTCC AGCCCACAAG CTCTACAGAA 
AATGATAATG CTTCTGCACA GGCAGAGCAC ATCCAAAGGA ATGTGTTTCA ATCTATGGTC 
CAGATGCAAC ACAGCGGAGA AACCCAGAAT CAAGTCAGTC TTTTTTCACC AGATGAAGAC 
ATGCTTGGAG TTCAGACAAG TGTAAGCCAA CAGCAAGGCA GTGGGCTTTT TCCCCCTGGA 
GACTTAATGA CTCTTCAGTC TAGTAACTTC ATGCAACAAT CCCCTCACTC ACACACTACA 
ATTTATCACT CACAGAACTC TATTGGGGAG AGCCAGAGTA TAAACCAGGA AACTCAGGGC 
TCTCTCTTCC ACTCTACCAA CACTATGGTG CAGCATCAGA ACACTGCATC TCAAGATCAG 
CTTCAGTCTC CACTTTTCCA CCCACAGAAC ACATTGTCTG TTTTGCAGAG TTCCTCTGTC 
ACTGCAGAGC AGACCTCGTC TAACCTCTTC CTGTCTCAAA GCACTATGAG CTCGCTGCAA 
AGTAATCCAG TGTCTCAAGA GAAGCAGCTC TCCTTTTTCA CAAGTCAAAA TTCACTCCCT 
CAGCTCCAAG CTGCTCCAAC CACAGAACAG CAAGCAGCCT TTCAACAGCA AGCCCAACTG 
TCTCACATTC AAAACTCAAT CATATCCCAA GAACAGCAGC AACAAGTTTC ACAAAATATA 
TATCAGCCAC AGAACCAACA AACAGCAATT TTCCAGGCAA CTCACTCAAT GGTTGGGATT 
CAGGGTAGTC CATCTTCTCA TGAGCAGAGA CAAAACATCC TATTCAGCAC ACAAAATTCA 
CTGTCTAACA TGGGATCGCC AGAGCAGCAG CAAAGCATGC TTTTTAATCC TGGACAGGAG 
CCAATGTCCA CCCAGGAGCA GCAGAACCAA GCTCTCTTTC ATTCCCAGAA CATGGAGTTC 
CAGTCTCAGA GCTCTGTTTC CACTCAGCAT GAACAGCAGC AGCAGTCTCC AATGTTTCAT 
AATTCTCCGC AACTTCAGCT TGTTCAGGCC TCCACAACCT CTCCAGAGCA ACAGGTTACA 
TTGTTTATGT CCCCCGTTTC CATGTCAGCC TTGCAGAGCA GCATCAACCA GCAAGACCTT 
CAGCAAGCCA GTATATACTC ACAGTCTATC CAGGGCAGTA CACCACCTCC TCCCCAGCAG 
TCTAATATAT ACCACAGTTC CACAAGTGGA GCAATGGGCC AAATTCAAGG TCAGCCAACA 
TCATCACCGC AGGCATCAGG AATGTTTTTG TTCGGGATTC AAAGCAATTG CGGTCAGCTG 
ATCTCCCAGG CTGCTGCTTC CATACCTGAT CAGATAATGG TTATGGCACA ATCAGGTCAA 
TCGCAAGGAG ATGGTCAATC TGTAACAGCA ATTCTATCCC AACAAATGAC AGATAATTCA 
TCAATGACAT CTGAACAGAG CATGGAGAAG ATCAGTGATT TGCTAGTTTC TCTGCAGAAC 
CAGGGGACCA GTTTGTCTGG CTCTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913618.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913675.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X8, mRNA.

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CloneID	OXa08899
Clone ID Related Accession (Same CDS sequence)	XM_004913618.3
Accession Version	XM_004913618.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAA CAACAACCAT TGTATCAGCT 
CAGCAAAATC TAGAAAATTC ATCAAATGCT AGACCTTTTT CACCCACTGT ATCCCATCTT 
CCCACTGATG CTGACCAACA GGCATCAATT CAAGTGCAGA CATTCAGCAC AGAACCTCTG 
GCCACCATCC AGACCCAAGA CATTGCCCAG CCTGGTAGTT TTCAGTCAGT GACATCTCCG 
GGCCAGGCTA ATGATTCCAT AATGCAGCAG ACTGGACAAT TTCAAACTAT GGATGGCCAA 
CCCAGGGACA CAATGCAGCC AGAAAACGCC ATTGTCACAC TTTCACAAAT GGCTGAAGTT 
ACTCCACAAG TGCAGCAGTC CACAATGCAA GAGCAAGCGC AGACATTACA ACAGCAGATT 
ACTACAGGCT TATTCACCTC CACAAGTAGC GTTAACCAGT TACAGAGTGC TATTAACCAA 
CTACAGGCTG GTAGCTTTCA GTCTAGTGTA AGTGGTGGTA ATACAAATGT AGATTTGGTC 
CAGCAGGTTC TGGACTCCCA GCAGTTGTCT TGCGTACTTT TTTCTGGACA AAGCGGAAAT 
GAAAGCAAGC AGGCCTCAGT TGGACAAGTT CAGGAGCGGA TCAGTGCTGG CATATTCCAA 
CCTGTTGCTT CAATACAAAA CAATGCAAAT TCTGCACTGT TTTCCACCCC TGAATCTGTG 
CATTCCAGAC CAGAAAACGT GCTTTCGGGA AGAACTGACA GCCTTCTCCA GCAGGCTGAA 
AATGCTTTGT CTAATCAACA GCAGCCCATG GAGACCACAG CAGCCCTAGT CATGGAGATG 
CAGCAAAGTA TTCTTCCCTC TGCTGCAACG ATTCAGCCTG ACCTTTTCCA GACACAAAAC 
TCTGGAACAT CAAACATTCA GTCACCTGTC TATCAGCACC AGCAAGGTTC TCATTTAATC 
CCTGGACTGT CCACTAATGA AGAGATGCCA ATGCAGTGTG ATATGTTTGC TAGTGCAGCA 
GTTCCTGGAA GTGAAAGCAG TGCAAACCAG CAGCAGGTAT CTTCATCTAG TTCAAATATG 
TTCCAGCCCA CAAGCTCTAC AGAAAATGAT AATGCTTCTG CACAGGCAGA GCACATCCAA 
AGGAATGTGT TTCAATCTAT GGTCCAGATG CAACACAGCG GAGAAACCCA GAATCAAGTC 
AGTCTTTTTT CACCAGATGA AGACATGCTT GGAGTTCAGA CAAGTGTAAG CCAACAGCAA 
GGCAGTGGGC TTTTTCCCCC TGGAGACTTA ATGACTCTTC AGTCTAGTAA CTTCATGCAA 
CAATCCCCTC ACTCACACAC TACAATTTAT CACTCACAGA ACTCTATTGG GGAGAGCCAG 
AGTATAAACC AGGAAACTCA GGGCTCTCTC TTCCACTCTA CCAACACTAT GGTGCAGCAT 
CAGAACACTG CATCTCAAGA TCAGCTTCAG TCTCCACTTT TCCACCCACA GAACACATTG 
TCTGTTTTGC AGAGTTCCTC TGTCACTGCA GAGCAGACCT CGTCTAACCT CTTCCTGTCT 
CAAAGCACTA TGAGCTCGCT GCAAAGTAAT CCAGTGTCTC AAGAGAAGCA GCTCTCCTTT 
TTCACAAGTC AAAATTCACT CCCTCAGCTC CAAGCTGCTC CAACCACAGA ACAGCAAGCA 
GCCTTTCAAC AGCAAGCCCA ACTGTCTCAC ATTCAAAACT CAATCATATC CCAAGAACAG 
CAGCAACAAG TTTCACAAAA TATATATCAG CCACAGAACC AACAAACAGC AATTTTCCAG 
GCAACTCACT CAATGGTTGG GATTCAGGGT AGTCCATCTT CTCATGAGCA GAGACAAAAC 
ATCCTATTCA GCACACAAAA TTCACTGTCT AACATGGGAT CGCCAGAGCA GCAGCAAAGC 
ATGCTTTTTA ATCCTGGACA GGAGCCAATG TCCACCCAGG AGCAGCAGAA CCAAGCTCTC 
TTTCATTCCC AGAACATGGA GTTCCAGTCT CAGAGCTCTG TTTCCACTCA GCATGAACAG 
CAGCAGCAGT CTCCAATGTT TCATAATTCT CCGCAACTTC AGCTTGTTCA GGCCTCCACA 
ACCTCTCCAG AGCAACAGGT TACATTGTTT ATGTCCCCCG TTTCCATGTC AGCCTTGCAG 
AGCAGCATCA ACCAGCAAGA CCTTCAGCAA GCCAGTATAT ACTCACAGTC TATCCAGGGC 
AGTACACCAC CTCCTCCCCA GCAGTCTAAT ATATACCACA GTTCCACAAG TGGAGCAATG 
GGCCAAATTC AAGGTCAGCC AACATCATCA CCGCAGGCAT CAGGAATGTT TTTGTTCGGG 
ATTCAAAGCA ATTGCGGTCA GCTGATCTCC CAGGCTGCTG CTTCCATACC TGATCAGATA 
ATGGTTATGG CACAATCAGG TCAATCGCAA GGAGATGGTC AATCTGTAAC AGCAATTCTA 
TCCCAACAAA TGACAGATAA TTCATCAATG ACATCTGAAC AGAGCATGGA GAAGATCAGT 
GATTTGCTAG TTTCTCTGCA GAACCAGGGG ACCAGTTTGT CTGGCTCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913675.1
CDS	282..4475
Translation	MRSLSEDVFSAPRLAHGCQHQNLITGVRQRESVYDLLPKELQLPSSRETSFAPMSQTSGGEAGSPPPAVIAADASSASSSSMGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSTAPSACNTDQTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913618.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913618.3

ORF Insert Sequence:

ATGCGCTCTC TCTCTGAAGA CGTCTTCTCA GCTCCAAGGC TTGCACATGG ATGCCAACAT 
CAGAATCTTA TAACAGGAGT AAGGCAAAGA GAATCTGTAT ATGACCTTCT CCCAAAGGAG 
TTACAGTTGC CCTCCTCACG AGAAACATCC TTCGCCCCAA TGAGTCAGAC AAGCGGAGGT 
GAGGCAGGCT CACCGCCCCC GGCTGTTATA GCTGCTGATG CCTCATCTGC CTCCTCTTCC 
TCAATGGGCG GTGCTTGCAG CTCCTTTACC ACCACTTCCA GCCCTTCTAT TTACTCTACC 
TCACTCACCG ATACCAAAGC TATGCAATCG GAGAGTTGCT CCTCTGCTCT GGGGGTAAGT 
AAACCAGGGG TAAGTGAACA GCTCTTAGCC AGTAATGCAG GCCAGCAGCA GACCGGGCCA 
CTAAGATGGA CGGTCCTTAA CATTTCGCCA CCACCCGAAG ACTTGCTCGA TAACAGTCAG 
ATGTCATGCC AAGATGAAGG GGGTGGACTG GAGTCGGAGC AGAGTTGCAG TATGTGGACT 
GAAGATTCCA CCTCCAATTT TAGCAACATG AGCACCCATT CTTACAATGA TAACACCGAG 
GTGCCTCGTA AGTCACGGAA AAGAAACCCA AAGCAAAGGC CTGGAGCGAA ACGGCAGGAT 
TCCAACATGG ATGTTTTTGA TGCTGATAGC GCCAAAGCTC CCCATTATGT GCTTTCCCAG 
CTCAACTCGG ACAGCAAAGG CCATACTGGA AATGGAGCTG CAGAGAATTC AAAAGGAGGA 
AAAAAGAGCC TGGTGTTGTG TGAGCAGTAT CCAGCTAAAG CTGAGGCAAA GGAACTGAAA 
ATAGTTGTGC AGCCAGAAAC ACAACATCGC GCCAGATACC TAACAGAGGG AAGTCGAGGA 
TCTGTAAAAG ATCGAACACA ACAAGGCTTT CCTACTGTGA AGCTGGAAGG CCATAATGAG 
TCAGTGGTGC TTCAAGTGTT TGTTGGTAAT GACTCTGGCA GAGTAAAGCC ACATGGATTC 
TACCAAGCCT GTAGGGTAAC TGGGAGAAAC ACCACTCCTT GCAAAGAAGT GGACATAGAG 
GGTACAACAG TTATTGAGGT TATGCTTGAC CCGAGCAATA TGATGACACT AGCAGTTGAT 
TGTGTTGGGA TCCTGAAGCT TCGGAATGCA GATGTAGAAG CACGGATAGG GGTTGCTGGC 
TCTAAGAAAA AGAGCACTCG TGCCAGACTT GTGTTTCGAG TAAACATCAC TCGTTCAGAT 
GGCTCAATTT TAACACTTCA AACACCTTCT TCACCTATTC TCTGTACCCA GCCTGCTGGT 
GTACCTGAAA TCCTAAAGAA GAGTTTACAT AGTTGCTCAG TGAAAGGGGA GGAAGAAGTC 
TTTCTCATTG GCAAAAACTT TTTAAAAGGA GCAAAAGTTG TATTCCAAGA GAATATCGCA 
GATGACAATT CCTGGAAAGC TGAAGCTGAA ATTGACATGG AGTTATTTCA TCAGAATCAC 
CTTATAGTGA AGGTTCCTTC TTTCCGCGAT CAGCACATTA GTTCCCCTGT TTCAGTGGTT 
ATGTTTGTGG TGACAAACGC AGGTCGATCT CATGATGTTC AGCCTTTCAC ATACACTCCA 
GATCAATCAA CAGCACCATC TGCCTGCAAC ACTGATCAAA CAACAACCAT TGTATCAGCT 
CAGCAAAATC TAGAAAATTC ATCAAATGCT AGACCTTTTT CACCCACTGT ATCCCATCTT 
CCCACTGATG CTGACCAACA GGCATCAATT CAAGTGCAGA CATTCAGCAC AGAACCTCTG 
GCCACCATCC AGACCCAAGA CATTGCCCAG CCTGGTAGTT TTCAGTCAGT GACATCTCCG 
GGCCAGGCTA ATGATTCCAT AATGCAGCAG ACTGGACAAT TTCAAACTAT GGATGGCCAA 
CCCAGGGACA CAATGCAGCC AGAAAACGCC ATTGTCACAC TTTCACAAAT GGCTGAAGTT 
ACTCCACAAG TGCAGCAGTC CACAATGCAA GAGCAAGCGC AGACATTACA ACAGCAGATT 
ACTACAGGCT TATTCACCTC CACAAGTAGC GTTAACCAGT TACAGAGTGC TATTAACCAA 
CTACAGGCTG GTAGCTTTCA GTCTAGTGTA AGTGGTGGTA ATACAAATGT AGATTTGGTC 
CAGCAGGTTC TGGACTCCCA GCAGTTGTCT TGCGTACTTT TTTCTGGACA AAGCGGAAAT 
GAAAGCAAGC AGGCCTCAGT TGGACAAGTT CAGGAGCGGA TCAGTGCTGG CATATTCCAA 
CCTGTTGCTT CAATACAAAA CAATGCAAAT TCTGCACTGT TTTCCACCCC TGAATCTGTG 
CATTCCAGAC CAGAAAACGT GCTTTCGGGA AGAACTGACA GCCTTCTCCA GCAGGCTGAA 
AATGCTTTGT CTAATCAACA GCAGCCCATG GAGACCACAG CAGCCCTAGT CATGGAGATG 
CAGCAAAGTA TTCTTCCCTC TGCTGCAACG ATTCAGCCTG ACCTTTTCCA GACACAAAAC 
TCTGGAACAT CAAACATTCA GTCACCTGTC TATCAGCACC AGCAAGGTTC TCATTTAATC 
CCTGGACTGT CCACTAATGA AGAGATGCCA ATGCAGTGTG ATATGTTTGC TAGTGCAGCA 
GTTCCTGGAA GTGAAAGCAG TGCAAACCAG CAGCAGGTAT CTTCATCTAG TTCAAATATG 
TTCCAGCCCA CAAGCTCTAC AGAAAATGAT AATGCTTCTG CACAGGCAGA GCACATCCAA 
AGGAATGTGT TTCAATCTAT GGTCCAGATG CAACACAGCG GAGAAACCCA GAATCAAGTC 
AGTCTTTTTT CACCAGATGA AGACATGCTT GGAGTTCAGA CAAGTGTAAG CCAACAGCAA 
GGCAGTGGGC TTTTTCCCCC TGGAGACTTA ATGACTCTTC AGTCTAGTAA CTTCATGCAA 
CAATCCCCTC ACTCACACAC TACAATTTAT CACTCACAGA ACTCTATTGG GGAGAGCCAG 
AGTATAAACC AGGAAACTCA GGGCTCTCTC TTCCACTCTA CCAACACTAT GGTGCAGCAT 
CAGAACACTG CATCTCAAGA TCAGCTTCAG TCTCCACTTT TCCACCCACA GAACACATTG 
TCTGTTTTGC AGAGTTCCTC TGTCACTGCA GAGCAGACCT CGTCTAACCT CTTCCTGTCT 
CAAAGCACTA TGAGCTCGCT GCAAAGTAAT CCAGTGTCTC AAGAGAAGCA GCTCTCCTTT 
TTCACAAGTC AAAATTCACT CCCTCAGCTC CAAGCTGCTC CAACCACAGA ACAGCAAGCA 
GCCTTTCAAC AGCAAGCCCA ACTGTCTCAC ATTCAAAACT CAATCATATC CCAAGAACAG 
CAGCAACAAG TTTCACAAAA TATATATCAG CCACAGAACC AACAAACAGC AATTTTCCAG 
GCAACTCACT CAATGGTTGG GATTCAGGGT AGTCCATCTT CTCATGAGCA GAGACAAAAC 
ATCCTATTCA GCACACAAAA TTCACTGTCT AACATGGGAT CGCCAGAGCA GCAGCAAAGC 
ATGCTTTTTA ATCCTGGACA GGAGCCAATG TCCACCCAGG AGCAGCAGAA CCAAGCTCTC 
TTTCATTCCC AGAACATGGA GTTCCAGTCT CAGAGCTCTG TTTCCACTCA GCATGAACAG 
CAGCAGCAGT CTCCAATGTT TCATAATTCT CCGCAACTTC AGCTTGTTCA GGCCTCCACA 
ACCTCTCCAG AGCAACAGGT TACATTGTTT ATGTCCCCCG TTTCCATGTC AGCCTTGCAG 
AGCAGCATCA ACCAGCAAGA CCTTCAGCAA GCCAGTATAT ACTCACAGTC TATCCAGGGC 
AGTACACCAC CTCCTCCCCA GCAGTCTAAT ATATACCACA GTTCCACAAG TGGAGCAATG 
GGCCAAATTC AAGGTCAGCC AACATCATCA CCGCAGGCAT CAGGAATGTT TTTGTTCGGG 
ATTCAAAGCA ATTGCGGTCA GCTGATCTCC CAGGCTGCTG CTTCCATACC TGATCAGATA 
ATGGTTATGG CACAATCAGG TCAATCGCAA GGAGATGGTC AATCTGTAAC AGCAATTCTA 
TCCCAACAAA TGACAGATAA TTCATCAATG ACATCTGAAC AGAGCATGGA GAAGATCAGT 
GATTTGCTAG TTTCTCTGCA GAACCAGGGG ACCAGTTTGT CTGGCTCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_004913620.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913677.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa08900
Clone ID Related Accession (Same CDS sequence)	XM_004913620.3 , XM_012962319.2 , XM_012962322.2 , XM_018093513.1
Accession Version	XM_004913620.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913677.1
CDS	233..4378
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_004913620.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913620.3

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962319.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817773.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa08900
Clone ID Related Accession (Same CDS sequence)	XM_004913620.3 , XM_012962319.2 , XM_012962322.2 , XM_018093513.1
Accession Version	XM_012962319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817773.1
CDS	295..4440
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962319.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962319.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_012962322.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817776.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X11, mRNA.

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CloneID	OXa08900
Clone ID Related Accession (Same CDS sequence)	XM_004913620.3 , XM_012962319.2 , XM_012962322.2 , XM_018093513.1
Accession Version	XM_012962322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817776.1
CDS	177..4322
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_012962322.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962322.2

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfat5 ( XM_018093513.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949002.1 Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X10, mRNA.

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CloneID	OXa08900
Clone ID Related Accession (Same CDS sequence)	XM_004913620.3 , XM_012962319.2 , XM_012962322.2 , XM_018093513.1
Accession Version	XM_018093513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear factor of activated T-cells 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949002.1
CDS	328..4473
Translation	MGGACSSFTTTSSPSIYSTSLTDTKAMQSESCSSALGVSKPGVSEQLLASNAGQQQTGPLRWTVLNISPPPEDLLDNSQMSCQDEGGGLESEQSCSMWTEDSTSNFSNMSTHSYNDNTEVPRKSRKRNPKQRPGAKRQDSNMDVFDADSAKAPHYVLSQLNSDSKGHTGNGAAENSKGGKKSLVLCEQYPAKAEAKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNESVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVMLDPSNMMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNITRSDGSILTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGAKVVFQENIADDNSWKAEAEIDMELFHQNHLIVKVPSFRDQHISSPVSVVMFVVTNAGRSHDVQPFTYTPDQSIPVNVLIKKEIPSPTQSCTFEDTMKATAPSACNTDQVSLLPSTLGSPLKSSGLIKNEDSAPMEMAADQRSSPLYKTTTIVSAQQNLENSSNARPFSPTVSHLPTDADQQASIQVQTFSTEPLATIQTQDIAQPGSFQSVTSPGQANDSIMQQTGQFQTMDGQPRDTMQPENAIVTLSQMAEVTPQVQQSTMQEQAQTLQQQITTGLFTSTSSVNQLQSAINQLQAGSFQSSVSGGNTNVDLVQQVLDSQQLSCVLFSGQSGNESKQASVGQVQERISAGIFQPVASIQNNANSALFSTPESVHSRPENVLSGRTDSLLQQAENALSNQQQPMETTAALVMEMQQSILPSAATIQPDLFQTQNSGTSNIQSPVYQHQQGSHLIPGLSTNEEMPMQCDMFASAAVPGSESSANQQQVSSSSSNMFQPTSSTENDNASAQAEHIQRNVFQSMVQMQHSGETQNQVSLFSPDEDMLGVQTSVSQQQGSGLFPPGDLMTLQSSNFMQQSPHSHTTIYHSQNSIGESQSINQETQGSLFHSTNTMVQHQNTASQDQLQSPLFHPQNTLSVLQSSSVTAEQTSSNLFLSQSTMSSLQSNPVSQEKQLSFFTSQNSLPQLQAAPTTEQQAAFQQQAQLSHIQNSIISQEQQQQVSQNIYQPQNQQTAIFQATHSMVGIQGSPSSHEQRQNILFSTQNSLSNMGSPEQQQSMLFNPGQEPMSTQEQQNQALFHSQNMEFQSQSSVSTQHEQQQQSPMFHNSPQLQLVQASTTSPEQQVTLFMSPVSMSALQSSINQQDLQQASIYSQSIQGSTPPPPQQSNIYHSSTSGAMGQIQGQPTSSPQASGMFLFGIQSNCGQLISQAAASIPDQIMVMAQSGQSQGDGQSVTAILSQQMTDNSSMTSEQSMEKISDLLVSLQNQGTSLSGSF

Target ORF information:

RefSeq Version	XM_018093513.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear factor of activated T-cells 5, tonicity-responsive (nfat5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093513.1

ORF Insert Sequence:

ATGGGCGGTG CTTGCAGCTC CTTTACCACC ACTTCCAGCC CTTCTATTTA CTCTACCTCA 
CTCACCGATA CCAAAGCTAT GCAATCGGAG AGTTGCTCCT CTGCTCTGGG GGTAAGTAAA 
CCAGGGGTAA GTGAACAGCT CTTAGCCAGT AATGCAGGCC AGCAGCAGAC CGGGCCACTA 
AGATGGACGG TCCTTAACAT TTCGCCACCA CCCGAAGACT TGCTCGATAA CAGTCAGATG 
TCATGCCAAG ATGAAGGGGG TGGACTGGAG TCGGAGCAGA GTTGCAGTAT GTGGACTGAA 
GATTCCACCT CCAATTTTAG CAACATGAGC ACCCATTCTT ACAATGATAA CACCGAGGTG 
CCTCGTAAGT CACGGAAAAG AAACCCAAAG CAAAGGCCTG GAGCGAAACG GCAGGATTCC 
AACATGGATG TTTTTGATGC TGATAGCGCC AAAGCTCCCC ATTATGTGCT TTCCCAGCTC 
AACTCGGACA GCAAAGGCCA TACTGGAAAT GGAGCTGCAG AGAATTCAAA AGGAGGAAAA 
AAGAGCCTGG TGTTGTGTGA GCAGTATCCA GCTAAAGCTG AGGCAAAGGA ACTGAAAATA 
GTTGTGCAGC CAGAAACACA ACATCGCGCC AGATACCTAA CAGAGGGAAG TCGAGGATCT 
GTAAAAGATC GAACACAACA AGGCTTTCCT ACTGTGAAGC TGGAAGGCCA TAATGAGTCA 
GTGGTGCTTC AAGTGTTTGT TGGTAATGAC TCTGGCAGAG TAAAGCCACA TGGATTCTAC 
CAAGCCTGTA GGGTAACTGG GAGAAACACC ACTCCTTGCA AAGAAGTGGA CATAGAGGGT 
ACAACAGTTA TTGAGGTTAT GCTTGACCCG AGCAATATGA TGACACTAGC AGTTGATTGT 
GTTGGGATCC TGAAGCTTCG GAATGCAGAT GTAGAAGCAC GGATAGGGGT TGCTGGCTCT 
AAGAAAAAGA GCACTCGTGC CAGACTTGTG TTTCGAGTAA ACATCACTCG TTCAGATGGC 
TCAATTTTAA CACTTCAAAC ACCTTCTTCA CCTATTCTCT GTACCCAGCC TGCTGGTGTA 
CCTGAAATCC TAAAGAAGAG TTTACATAGT TGCTCAGTGA AAGGGGAGGA AGAAGTCTTT 
CTCATTGGCA AAAACTTTTT AAAAGGAGCA AAAGTTGTAT TCCAAGAGAA TATCGCAGAT 
GACAATTCCT GGAAAGCTGA AGCTGAAATT GACATGGAGT TATTTCATCA GAATCACCTT 
ATAGTGAAGG TTCCTTCTTT CCGCGATCAG CACATTAGTT CCCCTGTTTC AGTGGTTATG 
TTTGTGGTGA CAAACGCAGG TCGATCTCAT GATGTTCAGC CTTTCACATA CACTCCAGAT 
CAATCCATTC CAGTAAATGT TCTTATAAAG AAAGAGATTC CCAGCCCAAC TCAGTCCTGC 
ACTTTTGAAG ACACTATGAA AGCAACAGCA CCATCTGCCT GCAACACTGA TCAAGTGAGT 
TTGCTCCCAA GTACTCTGGG TTCTCCTCTT AAATCAAGTG GTTTAATCAA AAATGAAGAT 
AGTGCTCCTA TGGAGATGGC TGCTGATCAG AGATCCTCTC CTCTATACAA AACAACAACC 
ATTGTATCAG CTCAGCAAAA TCTAGAAAAT TCATCAAATG CTAGACCTTT TTCACCCACT 
GTATCCCATC TTCCCACTGA TGCTGACCAA CAGGCATCAA TTCAAGTGCA GACATTCAGC 
ACAGAACCTC TGGCCACCAT CCAGACCCAA GACATTGCCC AGCCTGGTAG TTTTCAGTCA 
GTGACATCTC CGGGCCAGGC TAATGATTCC ATAATGCAGC AGACTGGACA ATTTCAAACT 
ATGGATGGCC AACCCAGGGA CACAATGCAG CCAGAAAACG CCATTGTCAC ACTTTCACAA 
ATGGCTGAAG TTACTCCACA AGTGCAGCAG TCCACAATGC AAGAGCAAGC GCAGACATTA 
CAACAGCAGA TTACTACAGG CTTATTCACC TCCACAAGTA GCGTTAACCA GTTACAGAGT 
GCTATTAACC AACTACAGGC TGGTAGCTTT CAGTCTAGTG TAAGTGGTGG TAATACAAAT 
GTAGATTTGG TCCAGCAGGT TCTGGACTCC CAGCAGTTGT CTTGCGTACT TTTTTCTGGA 
CAAAGCGGAA ATGAAAGCAA GCAGGCCTCA GTTGGACAAG TTCAGGAGCG GATCAGTGCT 
GGCATATTCC AACCTGTTGC TTCAATACAA AACAATGCAA ATTCTGCACT GTTTTCCACC 
CCTGAATCTG TGCATTCCAG ACCAGAAAAC GTGCTTTCGG GAAGAACTGA CAGCCTTCTC 
CAGCAGGCTG AAAATGCTTT GTCTAATCAA CAGCAGCCCA TGGAGACCAC AGCAGCCCTA 
GTCATGGAGA TGCAGCAAAG TATTCTTCCC TCTGCTGCAA CGATTCAGCC TGACCTTTTC 
CAGACACAAA ACTCTGGAAC ATCAAACATT CAGTCACCTG TCTATCAGCA CCAGCAAGGT 
TCTCATTTAA TCCCTGGACT GTCCACTAAT GAAGAGATGC CAATGCAGTG TGATATGTTT 
GCTAGTGCAG CAGTTCCTGG AAGTGAAAGC AGTGCAAACC AGCAGCAGGT ATCTTCATCT 
AGTTCAAATA TGTTCCAGCC CACAAGCTCT ACAGAAAATG ATAATGCTTC TGCACAGGCA 
GAGCACATCC AAAGGAATGT GTTTCAATCT ATGGTCCAGA TGCAACACAG CGGAGAAACC 
CAGAATCAAG TCAGTCTTTT TTCACCAGAT GAAGACATGC TTGGAGTTCA GACAAGTGTA 
AGCCAACAGC AAGGCAGTGG GCTTTTTCCC CCTGGAGACT TAATGACTCT TCAGTCTAGT 
AACTTCATGC AACAATCCCC TCACTCACAC ACTACAATTT ATCACTCACA GAACTCTATT 
GGGGAGAGCC AGAGTATAAA CCAGGAAACT CAGGGCTCTC TCTTCCACTC TACCAACACT 
ATGGTGCAGC ATCAGAACAC TGCATCTCAA GATCAGCTTC AGTCTCCACT TTTCCACCCA 
CAGAACACAT TGTCTGTTTT GCAGAGTTCC TCTGTCACTG CAGAGCAGAC CTCGTCTAAC 
CTCTTCCTGT CTCAAAGCAC TATGAGCTCG CTGCAAAGTA ATCCAGTGTC TCAAGAGAAG 
CAGCTCTCCT TTTTCACAAG TCAAAATTCA CTCCCTCAGC TCCAAGCTGC TCCAACCACA 
GAACAGCAAG CAGCCTTTCA ACAGCAAGCC CAACTGTCTC ACATTCAAAA CTCAATCATA 
TCCCAAGAAC AGCAGCAACA AGTTTCACAA AATATATATC AGCCACAGAA CCAACAAACA 
GCAATTTTCC AGGCAACTCA CTCAATGGTT GGGATTCAGG GTAGTCCATC TTCTCATGAG 
CAGAGACAAA ACATCCTATT CAGCACACAA AATTCACTGT CTAACATGGG ATCGCCAGAG 
CAGCAGCAAA GCATGCTTTT TAATCCTGGA CAGGAGCCAA TGTCCACCCA GGAGCAGCAG 
AACCAAGCTC TCTTTCATTC CCAGAACATG GAGTTCCAGT CTCAGAGCTC TGTTTCCACT 
CAGCATGAAC AGCAGCAGCA GTCTCCAATG TTTCATAATT CTCCGCAACT TCAGCTTGTT 
CAGGCCTCCA CAACCTCTCC AGAGCAACAG GTTACATTGT TTATGTCCCC CGTTTCCATG 
TCAGCCTTGC AGAGCAGCAT CAACCAGCAA GACCTTCAGC AAGCCAGTAT ATACTCACAG 
TCTATCCAGG GCAGTACACC ACCTCCTCCC CAGCAGTCTA ATATATACCA CAGTTCCACA 
AGTGGAGCAA TGGGCCAAAT TCAAGGTCAG CCAACATCAT CACCGCAGGC ATCAGGAATG 
TTTTTGTTCG GGATTCAAAG CAATTGCGGT CAGCTGATCT CCCAGGCTGC TGCTTCCATA 
CCTGATCAGA TAATGGTTAT GGCACAATCA GGTCAATCGC AAGGAGATGG TCAATCTGTA 
ACAGCAATTC TATCCCAACA AATGACAGAT AATTCATCAA TGACATCTGA ACAGAGCATG 
GAGAAGATCA GTGATTTGCT AGTTTCTCTG CAGAACCAGG GGACCAGTTT GTCTGGCTCT 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nfat5
Entrez Gene ID	100495532
Full Name	nuclear factor of activated T cells 5
Synonyms	nf-at5,nfatl1,nfatz,orebp,tonebp
General protein information	Preferred Names nuclear factor of activated T cells 5 Names nuclear factor of activated T-cells 5 nuclear factor of activated T-cells 5, tonicity-responsive
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	NFAT5	XP_001093880.2
Mus musculus (house mouse)	Nfat5	NP_061293.2
Xenopus tropicalis (tropical clawed frog)	nfat5	XP_002937623.2
Homo sapiens (human)	NFAT5	NP_619727.2
Pan troglodytes (chimpanzee)	NFAT5	XP_001168930.2
Canis lupus familiaris (dog)	NFAT5	XP_005620824.1
Gallus gallus (chicken)	NFAT5	NP_001185929.1
Bos taurus (cattle)	NFAT5	XP_002694885.1
Rattus norvegicus (Norway rat)	Nfat5	NP_001100895.1
Danio rerio (zebrafish)	LOC555802	XP_005174610.1

Next-day Shipping ORF Clones ( in default vector with tag)
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nfat5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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