nid1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nid1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nid1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa10603 XM_002935828.5
Latest version!
Xenopus tropicalis nidogen 1 (nid1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35580 XM_012963717.3
Latest version!
Xenopus tropicalis nidogen 1 (nid1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa44674 XM_018094264.1
Latest version!
Xenopus tropicalis nidogen 1 (nid1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
View Old Accession Versions >>
OXa10603 XM_002935828.4 Xenopus tropicalis nidogen 1 (nid1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35580 XM_012963717.2 Xenopus tropicalis nidogen 1 (nid1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa10603
    Clone ID Related Accession (Same CDS sequence) XM_002935828.4 , XM_002935828.5
    Accession Version XM_002935828.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nidogen-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935828.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGAACCAGAA AAGACAGCAT GCCAGCTCCA TCTAGATAGT
    ATTCTTGGCT CACCCTCTGT CAGAGGTGCA AGGCCAGTTG GTCAGTATAT TCCACAGTGT
    GATGAAAGTG GTAACTACAT TCCAAGGCAA TGCCATTCTG GCAGTGGATA TTGCTGGTGT
    GTGGACAGAT TTGGTAATGA ACTACCTGGT ACAAGAACAG GTCCTGGACT TAGTCCACCA
    TGTCTTGGTA TAGTTACTCA TCCTCCTCCT ACTGTTGGGC CCCCTGTGAG ACCAGATGTT
    GTTCCTCTGC CACCGGGAAC ACATTTGCTG TTTGCACAGA GTGGGAAGAT TGAGCATATC
    CCGCTAGAGG GAAACAACAT GAAGAAAACT GATGCCAAAA CCTTACTTCA TATCCCAGAT
    AAAGTTATAA TTGGTGTAGC CTTTGACTGT GTGGACAAGA CTGTATACTG GACGGATATC
    ACTGGACCTT CTATTAGTAG AGCCAGCATA AATGGCGGGG AACCAACTGC AATTATTAAA
    ACAGAGTTAG GAAGCCCTGA AGGAATAGCA GTTGACCATC TGGGTCGTAA CATTTTTTGG
    ACTGATTCTC TATTGAATAC AATTGAAGTA GCCAAGATGG ATGGAAGTAA TCGTCGAGTA
    TTATTTGATA CTGATCTAGA GAACCCCAGA GGAATAGTAG CTGATGCACC AAAAGGAAAT
    CTCTACTGGA CTGACTGGAA CAGAGCTGCC CCAAAGATTG AGACATCTTA TATGGATGGA
    ACCAACAGAA GAATTCTTGT TAAAGATGAT CTTGGTCTTC CAAATGGACT TACCATTGAC
    CCATATTCAT CAATGCTTTG TTGGATTGAC GCAGGAACTA AAAGAATGGA ATGCATGAAT
    CCCAGTCAGC CTGCACGCCG AAAAATTGTG GAAGGCATTC AGTATCCGTT TGGTATAACA
    AGTTATGGAA AGAACTTATT CTATACAGAC TGGAAGAGAG ATGCCATTGT AGCGATCGAT
    CGCACCATTT CCAAGGAGAG TGACAGCTAC CAGCCTCCCA AACGATCCCG TCTGTATGGA
    ATTACTACTG CTTATTCCCA GTGTCCTCAA GGTCAGAATT ACTGTGCGGT AAACAATGGA
    GGCTGCACAC ACCTTTGCTT GGCAACTCCT GGAGGTCGAT CCTGCCTCTG CCCTGAAAAC
    AGCATCGGAG TGGATTGCTT AGATAGAAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935874.2
    CDS189..3881
    Translation

    Target ORF information:

    RefSeq Version XM_002935828.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nidogen 1 (nid1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935828.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGAACCAGAA AAGACAGCAT GCCAGCTCCA TCTAGATAGT
    ATTCTTGGCT CACCCTCTGT CAGAGGTGCA AGGCCAGTTG GTCAGTATAT TCCACAGTGT
    GATGAAAGTG GTAACTACAT TCCAAGGCAA TGCCATTCTG GCAGTGGATA TTGCTGGTGT
    GTGGACAGAT TTGGTAATGA ACTACCTGGT ACAAGAACAG GTCCTGGACT TAGTCCACCA
    TGTCTTGGTA TAGTTACTCA TCCTCCTCCT ACTGTTGGGC CCCCTGTGAG ACCAGATGTT
    GTTCCTCTGC CACCGGGAAC ACATTTGCTG TTTGCACAGA GTGGGAAGAT TGAGCATATC
    CCGCTAGAGG GAAACAACAT GAAGAAAACT GATGCCAAAA CCTTACTTCA TATCCCAGAT
    AAAGTTATAA TTGGTGTAGC CTTTGACTGT GTGGACAAGA CTGTATACTG GACGGATATC
    ACTGGACCTT CTATTAGTAG AGCCAGCATA AATGGCGGGG AACCAACTGC AATTATTAAA
    ACAGAGTTAG GAAGCCCTGA AGGAATAGCA GTTGACCATC TGGGTCGTAA CATTTTTTGG
    ACTGATTCTC TATTGAATAC AATTGAAGTA GCCAAGATGG ATGGAAGTAA TCGTCGAGTA
    TTATTTGATA CTGATCTAGA GAACCCCAGA GGAATAGTAG CTGATGCACC AAAAGGAAAT
    CTCTACTGGA CTGACTGGAA CAGAGCTGCC CCAAAGATTG AGACATCTTA TATGGATGGA
    ACCAACAGAA GAATTCTTGT TAAAGATGAT CTTGGTCTTC CAAATGGACT TACCATTGAC
    CCATATTCAT CAATGCTTTG TTGGATTGAC GCAGGAACTA AAAGAATGGA ATGCATGAAT
    CCCAGTCAGC CTGCACGCCG AAAAATTGTG GAAGGCATTC AGTATCCGTT TGGTATAACA
    AGTTATGGAA AGAACTTATT CTATACAGAC TGGAAGAGAG ATGCCATTGT AGCGATCGAT
    CGCACCATTT CCAAGGAGAG TGACAGCTAC CAGCCTCCCA AACGATCCCG TCTGTATGGA
    ATTACTACTG CTTATTCCCA GTGTCCTCAA GGTCAGAATT ACTGTGCGGT AAACAATGGA
    GGCTGCACAC ACCTTTGCTT GGCAACTCCT GGAGGTCGAT CCTGCCTCTG CCCTGAAAAC
    AGCATCGGAG TGGATTGCTT AGATAGAAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35580
    Clone ID Related Accession (Same CDS sequence) XM_012963717.2 , XM_012963717.3
    Accession Version XM_012963717.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nidogen-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963717.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGGTCTTGGT ATAGTTACTC ATCCTCCTCC TACTGTTGGG
    CCCCCTGTGA GACCAGATGT TGTTCCTCTG CCACCGGGAA CACATTTGCT GTTTGCACAG
    AGTGGGAAGA TTGAGCATAT CCCGCTAGAG GGAAACAACA TGAAGAAAAC TGATGCCAAA
    ACCTTACTTC ATATCCCAGA TAAAGTTATA ATTGGTGTAG CCTTTGACTG TGTGGACAAG
    ACTGTATACT GGACGGATAT CACTGGACCT TCTATTAGTA GAGCCAGCAT AAATGGCGGG
    GAACCAACTG CAATTATTAA AACAGAGTTA GGAAGCCCTG AAGGAATAGC AGTTGACCAT
    CTGGGTCGTA ACATTTTTTG GACTGATTCT CTATTGAATA CAATTGAAGT AGCCAAGATG
    GATGGAAGTA ATCGTCGAGT ATTATTTGAT ACTGATCTAG AGAACCCCAG AGGAATAGTA
    GCTGATGCAC CAAAAGGAAA TCTCTACTGG ACTGACTGGA ACAGAGCTGC CCCAAAGATT
    GAGACATCTT ATATGGATGG AACCAACAGA AGAATTCTTG TTAAAGATGA TCTTGGTCTT
    CCAAATGGAC TTACCATTGA CCCATATTCA TCAATGCTTT GTTGGATTGA CGCAGGAACT
    AAAAGAATGG AATGCATGAA TCCCAGTCAG CCTGCACGCC GAAAAATTGT GGAAGGCATT
    CAGTATCCGT TTGGTATAAC AAGTTATGGA AAGAACTTAT TCTATACAGA CTGGAAGAGA
    GATGCCATTG TAGCGATCGA TCGCACCATT TCCAAGGAGA GTGACAGCTA CCAGCCTCCC
    AAACGATCCC GTCTGTATGG AATTACTACT GCTTATTCCC AGTGTCCTCA AGGTCAGAAT
    TACTGTGCGG TAAACAATGG AGGCTGCACA CACCTTTGCT TGGCAACTCC TGGAGGTCGA
    TCCTGCCTCT GCCCTGAAAA CAGCATCGGA GTGGATTGCT TAGATAGAAA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819171.1
    CDS189..3662
    Translation

    Target ORF information:

    RefSeq Version XM_012963717.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nidogen 1 (nid1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963717.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGGTCTTGGT ATAGTTACTC ATCCTCCTCC TACTGTTGGG
    CCCCCTGTGA GACCAGATGT TGTTCCTCTG CCACCGGGAA CACATTTGCT GTTTGCACAG
    AGTGGGAAGA TTGAGCATAT CCCGCTAGAG GGAAACAACA TGAAGAAAAC TGATGCCAAA
    ACCTTACTTC ATATCCCAGA TAAAGTTATA ATTGGTGTAG CCTTTGACTG TGTGGACAAG
    ACTGTATACT GGACGGATAT CACTGGACCT TCTATTAGTA GAGCCAGCAT AAATGGCGGG
    GAACCAACTG CAATTATTAA AACAGAGTTA GGAAGCCCTG AAGGAATAGC AGTTGACCAT
    CTGGGTCGTA ACATTTTTTG GACTGATTCT CTATTGAATA CAATTGAAGT AGCCAAGATG
    GATGGAAGTA ATCGTCGAGT ATTATTTGAT ACTGATCTAG AGAACCCCAG AGGAATAGTA
    GCTGATGCAC CAAAAGGAAA TCTCTACTGG ACTGACTGGA ACAGAGCTGC CCCAAAGATT
    GAGACATCTT ATATGGATGG AACCAACAGA AGAATTCTTG TTAAAGATGA TCTTGGTCTT
    CCAAATGGAC TTACCATTGA CCCATATTCA TCAATGCTTT GTTGGATTGA CGCAGGAACT
    AAAAGAATGG AATGCATGAA TCCCAGTCAG CCTGCACGCC GAAAAATTGT GGAAGGCATT
    CAGTATCCGT TTGGTATAAC AAGTTATGGA AAGAACTTAT TCTATACAGA CTGGAAGAGA
    GATGCCATTG TAGCGATCGA TCGCACCATT TCCAAGGAGA GTGACAGCTA CCAGCCTCCC
    AAACGATCCC GTCTGTATGG AATTACTACT GCTTATTCCC AGTGTCCTCA AGGTCAGAAT
    TACTGTGCGG TAAACAATGG AGGCTGCACA CACCTTTGCT TGGCAACTCC TGGAGGTCGA
    TCCTGCCTCT GCCCTGAAAA CAGCATCGGA GTGGATTGCT TAGATAGAAA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44674
    Clone ID Related Accession (Same CDS sequence) XM_018094264.1
    Accession Version XM_018094264.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nidogen-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGGATCG CCTCAAAGAG TGAATGGCAA AGTCAAAGGA
    AAGATTTACG TGGGAAGTAA TCCTGTTCCA GTGGTGTTTG AGGGCATTGA CCTTCACTCA
    TATGTTGTGG TTAATGATGG ACGAGCTTAT ACAGCTATTA GCACAATTGC TGATTCTTTG
    GGGTACTCTT TGCTTCCATT AGCATCGATT GGTGGAATTA TTGGATGGAT GTTTTCTGTG
    CAGCAACCAG GATATAAGAA TGGTTTCAGC ATTACAGGTG GAGAATTCAC ACGAAGAGCA
    GAGGTTACTT TCTTGCAGAG CAATAAGAAG CTAGTAATCC TGCAGAAGTT CAGTGGCATT
    GATGAACATG GTCATCTCAC CATCAGTACT GAGCTTGAAG GCAGTGTACC GGAGATACCA
    TATGGATCCA CAGTCCACAT TGAGCCTTAC ACAGAATTAT ATCAGACATC TAGTTCTGTT
    ATTACATCAA CATCTACCAG AGAGTACACT GTAGAGTTCC CACAAGATGG CTCGGAGACA
    TTTACTTATC AATGGAAACA GACCATCACA TTTAAGGAAT GTGTTCATGA CACCACTGCT
    CCAACAGTCC CTGCAATACA GCAGCTTTCA GTTGATAGAG TTTTTGTACT GTACAACAGA
    GATGAGAAAA TTCTCCGATA TGCTTCCAGT AACTCTATTG GGCCAATAAC TGACAGCCCT
    GTAGATACAA ATCGGAACCC ATGCTATGTA GGTACACATG TCTGTGACAC AAACGCTGTT
    TGCCGCCCAG CACAAGGAAA CCAATACACA TGTGAATGTA CTTCAGGTTT CCGTGGAGAT
    GGGAGGACAT GTTATGACAT CGATGAATGT CAGGAGCAAC AAGACATATG TGGGGATAAT
    GCCATATGTA ACAACCAGCC CGGAACATTT AGATGTGAAT GTAATGACGG ATATCAATTT
    TTGGAAGATG GAAGGACATG TGCAGCTGTG GATCGCCCTA CAAACCACTG CTTAACTGGA
    ACACACAACT GTGATATTGC AGACCGTGCT CGTTGTATAT ACACTGGAGG CTCTTCATAC
    ATATGTGCTT GTTTATCCGG ATATTCTGGA GATGGACGTG CATGTGAAGA TGTTGATGAA
    TGTGAAACCA GTCAATGCCA TCCAGATGCT GTGTGCTATA ACACTCCAGG GTCATTTTCA
    TGTCATTGTA ATCCTGGATA CCGTGGAAAT GGTTTCCAGT GCTTCCCAGG AGAACCAGAA
    AAGACAGCAT GCCAGCTCCA TCTAGATAGT ATTCTTGGCT CACCCTCTGT CAGAGGTGCA
    AGGCCAGTTG GTCAGTATAT TCCACAGTGT GATGAAAGTG GTAACTACAT TCCAAGGCAA
    TGCCATTCTG GCAGTGGATA TTGCTGGTGT GTGGACAGAT TTGGTAATGA ACTACCTGGT
    ACAAGAACAG GTCCTGGACT TAGTCCACCA TGTCTTGGTA TAGTTACTCA TCCTCCTCCT
    ACTGTTGGGC CCCCTGTGAG ACCAGATGTT GTTCCTCTGC CACCGGGAAC ACATTTGCTG
    TTTGCACAGA GTGGGAAGAT TGAGCATATC CCGCTAGAGG GAAACAACAT GAAGAAAACT
    GATGCCAAAA CCTTACTTCA TATCCCAGAT AAAGTTATAA TTGGTGTAGC CTTTGACTGT
    GTGGACAAGA CTGTATACTG GACGGATATC ACTGGACCTT CTATTAGTAG AGCCAGCATA
    AATGGCGGGG AACCAACTGC AATTATTAAA ACAGAGTTAG GAAGCCCTGA AGGAATAGCA
    GTTGACCATC TGGGTCGTAA CATTTTTTGG ACTGATTCTC TATTGAATAC AATTGAAGTA
    GCCAAGATGG ATGGAAGTAA TCGTCGAGTA TTATTTGATA CTGATCTAGA GAACCCCAGA
    GGAATAGTAG CTGATGCACC AAAAGGAAAT CTCTACTGGA CTGACTGGAA CAGAGCTGCC
    CCAAAGATTG AGACATCTTA TATGGATGGA ACCAACAGAA GAATTCTTGT TAAAGATGAT
    CTTGGTCTTC CAAATGGACT TACCATTGAC CCATATTCAT CAATGCTTTG TTGGATTGAC
    GCAGGAACTA AAAGAATGGA ATGCATGAAT CCCAGTCAGC CTGCACGCCG AAAAATTGTG
    GAAGGCATTC AGTATCCGTT TGGTATAACA AGTTATGGAA AGAACTTATT CTATACAGAC
    TGGAAGAGAG ATGCCATTGT AGCGATCGAT CGCACCATTT CCAAGGAGAG TGACAGCTAC
    CAGCCTCCCA AACGATCCCG TCTGTATGGA ATTACTACTG CTTATTCCCA GTGTCCTCAA
    GGTCAGAATT ACTGTGCGGT AAACAATGGA GGCTGCACAC ACCTTTGCTT GGCAACTCCT
    GGAGGTCGAT CCTGCCTCTG CCCTGAAAAC AGCATCGGAG TGGATTGCTT AGATAGAAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949753.1
    CDS170..3712
    Translation

    Target ORF information:

    RefSeq Version XM_018094264.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nidogen 1 (nid1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGGATCG CCTCAAAGAG TGAATGGCAA AGTCAAAGGA
    AAGATTTACG TGGGAAGTAA TCCTGTTCCA GTGGTGTTTG AGGGCATTGA CCTTCACTCA
    TATGTTGTGG TTAATGATGG ACGAGCTTAT ACAGCTATTA GCACAATTGC TGATTCTTTG
    GGGTACTCTT TGCTTCCATT AGCATCGATT GGTGGAATTA TTGGATGGAT GTTTTCTGTG
    CAGCAACCAG GATATAAGAA TGGTTTCAGC ATTACAGGTG GAGAATTCAC ACGAAGAGCA
    GAGGTTACTT TCTTGCAGAG CAATAAGAAG CTAGTAATCC TGCAGAAGTT CAGTGGCATT
    GATGAACATG GTCATCTCAC CATCAGTACT GAGCTTGAAG GCAGTGTACC GGAGATACCA
    TATGGATCCA CAGTCCACAT TGAGCCTTAC ACAGAATTAT ATCAGACATC TAGTTCTGTT
    ATTACATCAA CATCTACCAG AGAGTACACT GTAGAGTTCC CACAAGATGG CTCGGAGACA
    TTTACTTATC AATGGAAACA GACCATCACA TTTAAGGAAT GTGTTCATGA CACCACTGCT
    CCAACAGTCC CTGCAATACA GCAGCTTTCA GTTGATAGAG TTTTTGTACT GTACAACAGA
    GATGAGAAAA TTCTCCGATA TGCTTCCAGT AACTCTATTG GGCCAATAAC TGACAGCCCT
    GTAGATACAA ATCGGAACCC ATGCTATGTA GGTACACATG TCTGTGACAC AAACGCTGTT
    TGCCGCCCAG CACAAGGAAA CCAATACACA TGTGAATGTA CTTCAGGTTT CCGTGGAGAT
    GGGAGGACAT GTTATGACAT CGATGAATGT CAGGAGCAAC AAGACATATG TGGGGATAAT
    GCCATATGTA ACAACCAGCC CGGAACATTT AGATGTGAAT GTAATGACGG ATATCAATTT
    TTGGAAGATG GAAGGACATG TGCAGCTGTG GATCGCCCTA CAAACCACTG CTTAACTGGA
    ACACACAACT GTGATATTGC AGACCGTGCT CGTTGTATAT ACACTGGAGG CTCTTCATAC
    ATATGTGCTT GTTTATCCGG ATATTCTGGA GATGGACGTG CATGTGAAGA TGTTGATGAA
    TGTGAAACCA GTCAATGCCA TCCAGATGCT GTGTGCTATA ACACTCCAGG GTCATTTTCA
    TGTCATTGTA ATCCTGGATA CCGTGGAAAT GGTTTCCAGT GCTTCCCAGG AGAACCAGAA
    AAGACAGCAT GCCAGCTCCA TCTAGATAGT ATTCTTGGCT CACCCTCTGT CAGAGGTGCA
    AGGCCAGTTG GTCAGTATAT TCCACAGTGT GATGAAAGTG GTAACTACAT TCCAAGGCAA
    TGCCATTCTG GCAGTGGATA TTGCTGGTGT GTGGACAGAT TTGGTAATGA ACTACCTGGT
    ACAAGAACAG GTCCTGGACT TAGTCCACCA TGTCTTGGTA TAGTTACTCA TCCTCCTCCT
    ACTGTTGGGC CCCCTGTGAG ACCAGATGTT GTTCCTCTGC CACCGGGAAC ACATTTGCTG
    TTTGCACAGA GTGGGAAGAT TGAGCATATC CCGCTAGAGG GAAACAACAT GAAGAAAACT
    GATGCCAAAA CCTTACTTCA TATCCCAGAT AAAGTTATAA TTGGTGTAGC CTTTGACTGT
    GTGGACAAGA CTGTATACTG GACGGATATC ACTGGACCTT CTATTAGTAG AGCCAGCATA
    AATGGCGGGG AACCAACTGC AATTATTAAA ACAGAGTTAG GAAGCCCTGA AGGAATAGCA
    GTTGACCATC TGGGTCGTAA CATTTTTTGG ACTGATTCTC TATTGAATAC AATTGAAGTA
    GCCAAGATGG ATGGAAGTAA TCGTCGAGTA TTATTTGATA CTGATCTAGA GAACCCCAGA
    GGAATAGTAG CTGATGCACC AAAAGGAAAT CTCTACTGGA CTGACTGGAA CAGAGCTGCC
    CCAAAGATTG AGACATCTTA TATGGATGGA ACCAACAGAA GAATTCTTGT TAAAGATGAT
    CTTGGTCTTC CAAATGGACT TACCATTGAC CCATATTCAT CAATGCTTTG TTGGATTGAC
    GCAGGAACTA AAAGAATGGA ATGCATGAAT CCCAGTCAGC CTGCACGCCG AAAAATTGTG
    GAAGGCATTC AGTATCCGTT TGGTATAACA AGTTATGGAA AGAACTTATT CTATACAGAC
    TGGAAGAGAG ATGCCATTGT AGCGATCGAT CGCACCATTT CCAAGGAGAG TGACAGCTAC
    CAGCCTCCCA AACGATCCCG TCTGTATGGA ATTACTACTG CTTATTCCCA GTGTCCTCAA
    GGTCAGAATT ACTGTGCGGT AAACAATGGA GGCTGCACAC ACCTTTGCTT GGCAACTCCT
    GGAGGTCGAT CCTGCCTCTG CCCTGAAAAC AGCATCGGAG TGGATTGCTT AGATAGAAAT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10603
    Clone ID Related Accession (Same CDS sequence) XM_002935828.4 , XM_002935828.5
    Accession Version XM_002935828.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nidogen-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935828.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGAACCAGAA AAGACAGCAT GCCAGCTCCA TCTAGATAGT
    ATTCTTGGCT CACCCTCTGT CAGAGGTGCA AGGCCAGTTG GTCAGTATAT TCCACAGTGT
    GATGAAAGTG GTAACTACAT TCCAAGGCAA TGCCATTCTG GCAGTGGATA TTGCTGGTGT
    GTGGACAGAT TTGGTAATGA ACTACCTGGT ACAAGAACAG GTCCTGGACT TAGTCCACCA
    TGTCTTGGTA TAGTTACTCA TCCTCCTCCT ACTGTTGGGC CCCCTGTGAG ACCAGATGTT
    GTTCCTCTGC CACCGGGAAC ACATTTGCTG TTTGCACAGA GTGGGAAGAT TGAGCATATC
    CCGCTAGAGG GAAACAACAT GAAGAAAACT GATGCCAAAA CCTTACTTCA TATCCCAGAT
    AAAGTTATAA TTGGTGTAGC CTTTGACTGT GTGGACAAGA CTGTATACTG GACGGATATC
    ACTGGACCTT CTATTAGTAG AGCCAGCATA AATGGCGGGG AACCAACTGC AATTATTAAA
    ACAGAGTTAG GAAGCCCTGA AGGAATAGCA GTTGACCATC TGGGTCGTAA CATTTTTTGG
    ACTGATTCTC TATTGAATAC AATTGAAGTA GCCAAGATGG ATGGAAGTAA TCGTCGAGTA
    TTATTTGATA CTGATCTAGA GAACCCCAGA GGAATAGTAG CTGATGCACC AAAAGGAAAT
    CTCTACTGGA CTGACTGGAA CAGAGCTGCC CCAAAGATTG AGACATCTTA TATGGATGGA
    ACCAACAGAA GAATTCTTGT TAAAGATGAT CTTGGTCTTC CAAATGGACT TACCATTGAC
    CCATATTCAT CAATGCTTTG TTGGATTGAC GCAGGAACTA AAAGAATGGA ATGCATGAAT
    CCCAGTCAGC CTGCACGCCG AAAAATTGTG GAAGGCATTC AGTATCCGTT TGGTATAACA
    AGTTATGGAA AGAACTTATT CTATACAGAC TGGAAGAGAG ATGCCATTGT AGCGATCGAT
    CGCACCATTT CCAAGGAGAG TGACAGCTAC CAGCCTCCCA AACGATCCCG TCTGTATGGA
    ATTACTACTG CTTATTCCCA GTGTCCTCAA GGTCAGAATT ACTGTGCGGT AAACAATGGA
    GGCTGCACAC ACCTTTGCTT GGCAACTCCT GGAGGTCGAT CCTGCCTCTG CCCTGAAAAC
    AGCATCGGAG TGGATTGCTT AGATAGAAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935874.2
    CDS170..3862
    Translation

    Target ORF information:

    RefSeq Version XM_002935828.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nidogen 1 (nid1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935828.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGAACCAGAA AAGACAGCAT GCCAGCTCCA TCTAGATAGT
    ATTCTTGGCT CACCCTCTGT CAGAGGTGCA AGGCCAGTTG GTCAGTATAT TCCACAGTGT
    GATGAAAGTG GTAACTACAT TCCAAGGCAA TGCCATTCTG GCAGTGGATA TTGCTGGTGT
    GTGGACAGAT TTGGTAATGA ACTACCTGGT ACAAGAACAG GTCCTGGACT TAGTCCACCA
    TGTCTTGGTA TAGTTACTCA TCCTCCTCCT ACTGTTGGGC CCCCTGTGAG ACCAGATGTT
    GTTCCTCTGC CACCGGGAAC ACATTTGCTG TTTGCACAGA GTGGGAAGAT TGAGCATATC
    CCGCTAGAGG GAAACAACAT GAAGAAAACT GATGCCAAAA CCTTACTTCA TATCCCAGAT
    AAAGTTATAA TTGGTGTAGC CTTTGACTGT GTGGACAAGA CTGTATACTG GACGGATATC
    ACTGGACCTT CTATTAGTAG AGCCAGCATA AATGGCGGGG AACCAACTGC AATTATTAAA
    ACAGAGTTAG GAAGCCCTGA AGGAATAGCA GTTGACCATC TGGGTCGTAA CATTTTTTGG
    ACTGATTCTC TATTGAATAC AATTGAAGTA GCCAAGATGG ATGGAAGTAA TCGTCGAGTA
    TTATTTGATA CTGATCTAGA GAACCCCAGA GGAATAGTAG CTGATGCACC AAAAGGAAAT
    CTCTACTGGA CTGACTGGAA CAGAGCTGCC CCAAAGATTG AGACATCTTA TATGGATGGA
    ACCAACAGAA GAATTCTTGT TAAAGATGAT CTTGGTCTTC CAAATGGACT TACCATTGAC
    CCATATTCAT CAATGCTTTG TTGGATTGAC GCAGGAACTA AAAGAATGGA ATGCATGAAT
    CCCAGTCAGC CTGCACGCCG AAAAATTGTG GAAGGCATTC AGTATCCGTT TGGTATAACA
    AGTTATGGAA AGAACTTATT CTATACAGAC TGGAAGAGAG ATGCCATTGT AGCGATCGAT
    CGCACCATTT CCAAGGAGAG TGACAGCTAC CAGCCTCCCA AACGATCCCG TCTGTATGGA
    ATTACTACTG CTTATTCCCA GTGTCCTCAA GGTCAGAATT ACTGTGCGGT AAACAATGGA
    GGCTGCACAC ACCTTTGCTT GGCAACTCCT GGAGGTCGAT CCTGCCTCTG CCCTGAAAAC
    AGCATCGGAG TGGATTGCTT AGATAGAAAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35580
    Clone ID Related Accession (Same CDS sequence) XM_012963717.2 , XM_012963717.3
    Accession Version XM_012963717.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nidogen-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGGTCTTGGT ATAGTTACTC ATCCTCCTCC TACTGTTGGG
    CCCCCTGTGA GACCAGATGT TGTTCCTCTG CCACCGGGAA CACATTTGCT GTTTGCACAG
    AGTGGGAAGA TTGAGCATAT CCCGCTAGAG GGAAACAACA TGAAGAAAAC TGATGCCAAA
    ACCTTACTTC ATATCCCAGA TAAAGTTATA ATTGGTGTAG CCTTTGACTG TGTGGACAAG
    ACTGTATACT GGACGGATAT CACTGGACCT TCTATTAGTA GAGCCAGCAT AAATGGCGGG
    GAACCAACTG CAATTATTAA AACAGAGTTA GGAAGCCCTG AAGGAATAGC AGTTGACCAT
    CTGGGTCGTA ACATTTTTTG GACTGATTCT CTATTGAATA CAATTGAAGT AGCCAAGATG
    GATGGAAGTA ATCGTCGAGT ATTATTTGAT ACTGATCTAG AGAACCCCAG AGGAATAGTA
    GCTGATGCAC CAAAAGGAAA TCTCTACTGG ACTGACTGGA ACAGAGCTGC CCCAAAGATT
    GAGACATCTT ATATGGATGG AACCAACAGA AGAATTCTTG TTAAAGATGA TCTTGGTCTT
    CCAAATGGAC TTACCATTGA CCCATATTCA TCAATGCTTT GTTGGATTGA CGCAGGAACT
    AAAAGAATGG AATGCATGAA TCCCAGTCAG CCTGCACGCC GAAAAATTGT GGAAGGCATT
    CAGTATCCGT TTGGTATAAC AAGTTATGGA AAGAACTTAT TCTATACAGA CTGGAAGAGA
    GATGCCATTG TAGCGATCGA TCGCACCATT TCCAAGGAGA GTGACAGCTA CCAGCCTCCC
    AAACGATCCC GTCTGTATGG AATTACTACT GCTTATTCCC AGTGTCCTCA AGGTCAGAAT
    TACTGTGCGG TAAACAATGG AGGCTGCACA CACCTTTGCT TGGCAACTCC TGGAGGTCGA
    TCCTGCCTCT GCCCTGAAAA CAGCATCGGA GTGGATTGCT TAGATAGAAA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819171.1
    CDS172..3645
    Translation

    Target ORF information:

    RefSeq Version XM_012963717.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nidogen 1 (nid1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963717.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTATATT TTAAGCCCAG AAGCCTGTTT TTTGGAGGGC TGTTACATGT TTTGTTCTGC 
    TTGTTGCCTA ACCCAGGGAA CTTCATAAAA GTCAGTCAGT TCTTCCCTTA CGGACCTGAT
    GTTGGTGACC TTCTTTTAAC TCCTGGAGAT GATTCTCAAT CGGAACCTTT ACGACCCAAT
    CCAGCGCTTC ATTTCTATGA CGAAACAATC TCTACCCTAT TCGTAACAAC TAATGGGATG
    ATTGCAGCAA GCAATCCGCC ACCAGAAAGC GATTATCTTG AGCAGTTCCC TCCAGATTTT
    GGTCTGATTG CCCCTTTTCT GTCAGACATT GATACCACAC AAGGTGGAAA TGTGTTTTAC
    AGAGAAGACA CGTCACAAGA CGTCTTACAT TTGGCATCTA AACACATTAT TAGAGGGTTT
    CCAGGAAGTT CCTTTAAGCC AACTACTGCT CTTGTTGTCA CTTGGGAAGA TGTGTTACCC
    TACTACTCTC AGCGAGGGGA TACTATTCCA AAAAACAAGG GAAACACTTT TCAGGCTGTT
    TTAGCATCAG ATGAGTCAAA ATCCTATGCT ATTTTCCTCT ACCCAGAGGA TGGCTTAAAC
    TTTTATGAGA CACAATCAAA GAATGATCGC AATGAGGAAA ACTCAGCCAG AGTTGGATTT
    AGTAAAGGGT CAGAAAGAGG GTTTTTCTGG ACGACGACTC AAGGACCGCA TAATGAGTTT
    GCCAGTGATT ATGATTCCCT GGAAATATTA TGCTGGAAAA CCAATTCAGG TATGCAGGGA
    GTCTGGATTT ATGAAATTGG AGCTCCAAAT CTCAGATCTG TTGTGGCTGC TGATGTACAG
    CTCTCTGCTA CTCTTGATGA AAAAGAACAA GAGGAAAACC ATTTTGAAGA GACAGAAGAT
    TTTACTGACC ATTATGTAAC TGAACCAGAT TATGAAAATA ATTCACTAAA ACCTAGCATT
    CCGGGTCCTA CTTCAATTTC TGTTGACCCC TATCAAGGCC AGAAGGACGT TATTTTGGGA
    AATGTGAATT TTCAAGTTCC ATCTTTTCCA CAAAATAACC CACAAGTTGT GATTGTTGAT
    GATGAACTAG AGGACACTGG AGTAGTGTTC CAGTATAATA CAGGAAGTCA GCAGACCTGT
    GCTAACAATC GCCATCAGTG CTCCAGTCAG GCATTTTGCA AAGATTTTTC AACTGGATTT
    TGTTGTAGTT GCATTCCTGG CTATTATGGT AATGGAAGGC AGTGTGTGGC AGAAGGATCG
    CCTCAAAGAG TGAATGGCAA AGTCAAAGGA AAGATTTACG TGGGAAGTAA TCCTGTTCCA
    GTGGTGTTTG AGGGCATTGA CCTTCACTCA TATGTTGTGG TTAATGATGG ACGAGCTTAT
    ACAGCTATTA GCACAATTGC TGATTCTTTG GGGTACTCTT TGCTTCCATT AGCATCGATT
    GGTGGAATTA TTGGATGGAT GTTTTCTGTG CAGCAACCAG GATATAAGAA TGGTTTCAGC
    ATTACAGGTG GAGAATTCAC ACGAAGAGCA GAGGTTACTT TCTTGCAGAG CAATAAGAAG
    CTAGTAATCC TGCAGAAGTT CAGTGGCATT GATGAACATG GTCATCTCAC CATCAGTACT
    GAGCTTGAAG GCAGTGTACC GGAGATACCA TATGGATCCA CAGTCCACAT TGAGCCTTAC
    ACAGAATTAT ATCAGACATC TAGTTCTGTT ATTACATCAA CATCTACCAG AGAGTACACT
    GTAGAGTTCC CACAAGATGG CTCGGAGACA TTTACTTATC AATGGAAACA GACCATCACA
    TTTAAGGAAT GTGTTCATGA CACCACTGCT CCAACAGTCC CTGCAATACA GCAGCTTTCA
    GTTGATAGAG TTTTTGTACT GTACAACAGA GATGAGAAAA TTCTCCGATA TGCTTCCAGT
    AACTCTATTG GGCCAATAAC TGACAGCCCT GTAGATACAA ATCGGAACCC ATGCTATGTA
    GGTACACATG TCTGTGACAC AAACGCTGTT TGCCGCCCAG CACAAGGAAA CCAATACACA
    TGTGAATGTA CTTCAGGTTT CCGTGGAGAT GGGAGGACAT GTTATGACAT CGATGAATGT
    CAGGAGCAAC AAGACATATG TGGGGATAAT GCCATATGTA ACAACCAGCC CGGAACATTT
    AGATGTGAAT GTAATGACGG ATATCAATTT TTGGAAGATG GAAGGACATG TGCAGCTGTG
    GATCGCCCTA CAAACCACTG CTTAACTGGA ACACACAACT GTGATATTGC AGACCGTGCT
    CGTTGTATAT ACACTGGAGG CTCTTCATAC ATATGTGCTT GTTTATCCGG ATATTCTGGA
    GATGGACGTG CATGTGAAGA TGTTGATGAA TGTGAAACCA GTCAATGCCA TCCAGATGCT
    GTGTGCTATA ACACTCCAGG GTCATTTTCA TGTCATTGTA ATCCTGGATA CCGTGGAAAT
    GGTTTCCAGT GCTTCCCAGG AGGTCTTGGT ATAGTTACTC ATCCTCCTCC TACTGTTGGG
    CCCCCTGTGA GACCAGATGT TGTTCCTCTG CCACCGGGAA CACATTTGCT GTTTGCACAG
    AGTGGGAAGA TTGAGCATAT CCCGCTAGAG GGAAACAACA TGAAGAAAAC TGATGCCAAA
    ACCTTACTTC ATATCCCAGA TAAAGTTATA ATTGGTGTAG CCTTTGACTG TGTGGACAAG
    ACTGTATACT GGACGGATAT CACTGGACCT TCTATTAGTA GAGCCAGCAT AAATGGCGGG
    GAACCAACTG CAATTATTAA AACAGAGTTA GGAAGCCCTG AAGGAATAGC AGTTGACCAT
    CTGGGTCGTA ACATTTTTTG GACTGATTCT CTATTGAATA CAATTGAAGT AGCCAAGATG
    GATGGAAGTA ATCGTCGAGT ATTATTTGAT ACTGATCTAG AGAACCCCAG AGGAATAGTA
    GCTGATGCAC CAAAAGGAAA TCTCTACTGG ACTGACTGGA ACAGAGCTGC CCCAAAGATT
    GAGACATCTT ATATGGATGG AACCAACAGA AGAATTCTTG TTAAAGATGA TCTTGGTCTT
    CCAAATGGAC TTACCATTGA CCCATATTCA TCAATGCTTT GTTGGATTGA CGCAGGAACT
    AAAAGAATGG AATGCATGAA TCCCAGTCAG CCTGCACGCC GAAAAATTGT GGAAGGCATT
    CAGTATCCGT TTGGTATAAC AAGTTATGGA AAGAACTTAT TCTATACAGA CTGGAAGAGA
    GATGCCATTG TAGCGATCGA TCGCACCATT TCCAAGGAGA GTGACAGCTA CCAGCCTCCC
    AAACGATCCC GTCTGTATGG AATTACTACT GCTTATTCCC AGTGTCCTCA AGGTCAGAAT
    TACTGTGCGG TAAACAATGG AGGCTGCACA CACCTTTGCT TGGCAACTCC TGGAGGTCGA
    TCCTGCCTCT GCCCTGAAAA CAGCATCGGA GTGGATTGCT TAGATAGAAA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.