NID1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NID1
Entrez Gene ID	4811
Full Name	nidogen 1
Synonyms	NID
General protein information	Preferred Names nidogen 1 Names nidogen-1 NID-1 enactin entactin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.3
Summary	This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008].
Disorder MIM:	131390

mRNA and Protein(s)

mRNA	Protein	Name
NM_002508.2	NP_002499.2	nidogen-1 precursor
NM_002508.3	NP_002499.2	nidogen-1 precursor
XM_011544195.2	XP_011542497.1	nidogen-1 isoform X1
XM_011544195.3	XP_011542497.1	nidogen-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NID1	XP_001156001.1
Gallus gallus (chicken)	NID1	XP_419556.3
	NID1	XP_001100451.1
Canis lupus familiaris (dog)	NID1	XP_546076.2
Bos taurus (cattle)	NID1	NP_001094625.1
Homo sapiens (human)	NID1	NP_002499.2
Mus musculus (house mouse)	Nid1	NP_035047.2
Rattus norvegicus (Norway rat)	Nid1	XP_213954.4
Danio rerio (zebrafish)	nid1a	XP_691156.5
Xenopus tropicalis (tropical clawed frog)	nid1	XP_002935874.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Identification of Nidogen 1 as a lung metastasis protein through secretome analysis.
Ale?kovi? M, Wei Y, LeRoy G, Sidoli S, Liu DD, Garcia BA, Kang Y
Genes & development31(14)1439-1455(2017 Jul)

NID1, a new regulator of EMT required for metastasis and chemoresistance of ovarian cancer cells.
Zhou Y, Zhu Y, Fan X, Zhang C, Wang Y, Zhang L, Zhang H, Wen T, Zhang K, Huo X, Jiang X, Bu Y, Zhang Y
Oncotarget8(20)33110-33121(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nidogen 1 (NID1) was confirmed to promote lung metastasis of breast cancer and melanoma.
Title: Identification of Nidogen 1 as a lung metastasis protein through secretome analysis.

nidogen-1 degraded by CatS can be quantified in serum by the NIC assay. The current data strongly suggest that cathepsin-S degradation of nidogen-1 is strongly associated with NSCLC, which needs validation in larger clinical cohorts.
Title: Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer.

Differential protein expression of collagen IV, laminin alpha2, and nidogen-1 indicated basal lamina remodeling develops in ischemic failing versus nonfailing human hearts.
Title: Differential protein expression and basal lamina remodeling in human heart failure.

NID1 was confirmed as a target gene of MiR-192/215 in Hirschsprung's disease patients.
Title: Nidogen-1 is a common target of microRNAs MiR-192/215 in the pathogenesis of Hirschsprung's disease.

Increase of NID1 protein expression is associated with endometrial cancer invasion.
Title: Nidogen 1 and Nuclear Protein 1: novel targets of ETV5 transcription factor involved in endometrial cancer invasion.

The following NID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18198	NM_002508.3 Latest version!	Homo sapiens nidogen 1 (NID1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu59277	XM_011544195.3 Latest version!	Homo sapiens nidogen 1 (NID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu18198	NM_002508.2	Homo sapiens nidogen 1 (NID1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu59277	XM_011544195.2	Homo sapiens nidogen 1 (NID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NID1 ( NM_002508.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002499.2 Homo sapiens nidogen 1 (NID1), mRNA.

Price & Availability↑

CloneID	OHu18198
Clone ID Related Accession (Same CDS sequence)	NM_002508.2 , NM_002508.3
Accession Version	NM_002508.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	nidogen-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN999415.1, M30269.1, AL122018.37, AL139161.8, CN267119.1, DA269707.1 and BQ549657.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2006 this sequence version replaced NM_002508.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.79865.1, SRR1660805.191824.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGAT ATTGATGAAT GTTCAGAACA ACCCTCAGTG 
TGTGGGAGCC ACACAATCTG CAATAATCAC CCAGGAACCT TCCGCTGCGA GTGTGTGGAG 
GGCTACCAGT TTTCAGATGA GGGAACGTGT GTGGCTGTCG TGGACCAGCG CCCCATCAAC 
TACTGTGAAA CTGGCCTTCA TAACTGCGAC ATACCCCAGC GGGCCCAGTG TATCTACACA 
GGAGGCTCCT CCTACACCTG TTCCTGCTTG CCAGGCTTTT CTGGGGATGG CCAAGCCTGC 
CAAGATGTAG ATGAATGCCA GCCAAGCCGA TGTCACCCTG ACGCCTTCTG CTACAACACT 
CCAGGCTCTT TCACGTGCCA GTGCAAACCT GGTTATCAGG GAGACGGCTT CCGTTGCGTG 
CCCGGAGAGG TGGAGAAAAC CCGGTGCCAG CACGAGCGAG AACACATTCT CGGGGCAGCG 
GGGGCGACAG ACCCACAGCG ACCCATTCCT CCGGGGCTGT TCGTTCCTGA GTGCGATGCG 
CACGGGCACT ACGCGCCCAC CCAGTGCCAC GGCAGCACCG GCTACTGCTG GTGCGTGGAT 
CGCGACGGCC GCGAGGTGGA GGGCACCAGG ACCAGGCCCG GGATGACGCC CCCGTGTCTG 
AGTACAGTGG CTCCCCCGAT TCACCAAGGA CCTGCGGTGC CTACCGCCGT GATCCCCTTG 
CCTCCTGGGA CCCATTTACT CTTTGCCCAG ACTGGGAAGA TTGAGCGCCT GCCCCTGGAG 
GGAAATACCA TGAGGAAGAC AGAAGCAAAG GCGTTCCTTC ATGTCCCGGC TAAAGTCATC 
ATTGGACTGG CCTTTGACTG CGTGGACAAG ATGGTTTACT GGACGGACAT CACTGAGCCT 
TCCATTGGGA GAGCTAGTCT ACATGGTGGA GAGCCAACCA CCATCATTAG ACAAGATCTT 
GGAAGTCCAG AAGGTATCGC TGTTGATCAC CTTGGCCGCA ACATCTTCTG GACAGACTCT 
AACCTGGATC GAATAGAAGT GGCGAAGCTG GACGGCACGC AGCGCCGGGT GCTCTTTGAG 
ACTGACTTGG TGAATCCCAG AGGCATTGTA ACGGATTCCG TGAGAGGGAA CCTTTACTGG 
ACAGACTGGA ACAGAGATAA CCCCAAGATT GAAACTTCCT ACATGGACGG CACGAACCGG 
AGGATCCTTG TGCAGGATGA CCTGGGCTTG CCCAATGGAC TGACCTTCGA TGCGTTCTCA 
TCTCAGCTCT GCTGGGTGGA TGCAGGCACC AATCGGGCGG AATGCCTGAA CCCCAGTCAG 
CCCAGCAGAC GCAAGGCTCT CGAAGGGCTC CAGTATCCTT TTGCTGTGAC GAGCTACGGG 
AAGAATCTGT ATTTCACAGA CTGGAAGATG AATTCCGTGG TTGCTCTCGA TCTTGCAATT 
TCCAAGGAGA CGGATGCTTT CCAACCCCAC AAGCAGACCC GGCTGTATGG CATCACCACG 
GCCCTGTCTC AGTGTCCGCA AGGCCATAAC TACTGCTCAG TGAACAATGG CGGCTGCACC 
CACCTATGCT TGGCCACCCC AGGGAGCAGG ACCTGCCGTT GCCCTGACAA CACCTTGGGA 
GTTGACTGTA TCGAACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002499.2
CDS	103..3846
Translation	MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDLELEDGDDFVSPALELSGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESVENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVHAECRDYATGFCCSCVAGYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPIVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPVGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIPFGSSVHIEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSRPALPSTQQLSVDSVFVLYNQEEKILRYALSNSIGPVREGSPDALQNPCYIGTHGCDTNAACRPGPRTQFTCECSIGFRGDGRTCYDIDECSEQPSVCGSHTICNNHPGTFRCECVEGYQFSDEGTCVAVVDQRPINYCETGLHNCDIPQRAQCIYTGGSSYTCSCLPGFSGDGQACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCVPGEVEKTRCQHEREHILGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTRTRPGMTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNPKIETSYMDGTNRRILVQDDLGLPNGLTFDAFSSQLCWVDAGTNRAECLNPSQPSRRKALEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIEQK

Target ORF information:

RefSeq Version	NM_002508.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 1 (NID1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002508.2

ORF Insert Sequence:

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGAT ATTGATGAAT GTTCAGAACA ACCCTCAGTG 
TGTGGGAGCC ACACAATCTG CAATAATCAC CCAGGAACCT TCCGCTGCGA GTGTGTGGAG 
GGCTACCAGT TTTCAGATGA GGGAACGTGT GTGGCTGTCG TGGACCAGCG CCCCATCAAC 
TACTGTGAAA CTGGCCTTCA TAACTGCGAC ATACCCCAGC GGGCCCAGTG TATCTACACA 
GGAGGCTCCT CCTACACCTG TTCCTGCTTG CCAGGCTTTT CTGGGGATGG CCAAGCCTGC 
CAAGATGTAG ATGAATGCCA GCCAAGCCGA TGTCACCCTG ACGCCTTCTG CTACAACACT 
CCAGGCTCTT TCACGTGCCA GTGCAAACCT GGTTATCAGG GAGACGGCTT CCGTTGCGTG 
CCCGGAGAGG TGGAGAAAAC CCGGTGCCAG CACGAGCGAG AACACATTCT CGGGGCAGCG 
GGGGCGACAG ACCCACAGCG ACCCATTCCT CCGGGGCTGT TCGTTCCTGA GTGCGATGCG 
CACGGGCACT ACGCGCCCAC CCAGTGCCAC GGCAGCACCG GCTACTGCTG GTGCGTGGAT 
CGCGACGGCC GCGAGGTGGA GGGCACCAGG ACCAGGCCCG GGATGACGCC CCCGTGTCTG 
AGTACAGTGG CTCCCCCGAT TCACCAAGGA CCTGCGGTGC CTACCGCCGT GATCCCCTTG 
CCTCCTGGGA CCCATTTACT CTTTGCCCAG ACTGGGAAGA TTGAGCGCCT GCCCCTGGAG 
GGAAATACCA TGAGGAAGAC AGAAGCAAAG GCGTTCCTTC ATGTCCCGGC TAAAGTCATC 
ATTGGACTGG CCTTTGACTG CGTGGACAAG ATGGTTTACT GGACGGACAT CACTGAGCCT 
TCCATTGGGA GAGCTAGTCT ACATGGTGGA GAGCCAACCA CCATCATTAG ACAAGATCTT 
GGAAGTCCAG AAGGTATCGC TGTTGATCAC CTTGGCCGCA ACATCTTCTG GACAGACTCT 
AACCTGGATC GAATAGAAGT GGCGAAGCTG GACGGCACGC AGCGCCGGGT GCTCTTTGAG 
ACTGACTTGG TGAATCCCAG AGGCATTGTA ACGGATTCCG TGAGAGGGAA CCTTTACTGG 
ACAGACTGGA ACAGAGATAA CCCCAAGATT GAAACTTCCT ACATGGACGG CACGAACCGG 
AGGATCCTTG TGCAGGATGA CCTGGGCTTG CCCAATGGAC TGACCTTCGA TGCGTTCTCA 
TCTCAGCTCT GCTGGGTGGA TGCAGGCACC AATCGGGCGG AATGCCTGAA CCCCAGTCAG 
CCCAGCAGAC GCAAGGCTCT CGAAGGGCTC CAGTATCCTT TTGCTGTGAC GAGCTACGGG 
AAGAATCTGT ATTTCACAGA CTGGAAGATG AATTCCGTGG TTGCTCTCGA TCTTGCAATT 
TCCAAGGAGA CGGATGCTTT CCAACCCCAC AAGCAGACCC GGCTGTATGG CATCACCACG 
GCCCTGTCTC AGTGTCCGCA AGGCCATAAC TACTGCTCAG TGAACAATGG CGGCTGCACC 
CACCTATGCT TGGCCACCCC AGGGAGCAGG ACCTGCCGTT GCCCTGACAA CACCTTGGGA 
GTTGACTGTA TCGAACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID1 ( NM_002508.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002499.2 Homo sapiens nidogen 1 (NID1), mRNA.

Price & Availability↑

CloneID	OHu18198
Clone ID Related Accession (Same CDS sequence)	NM_002508.2 , NM_002508.3
Accession Version	NM_002508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	nidogen-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M30269.1, AL122018.37, AL139161.8, CN267119.1, DA269707.1 and BQ549657.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002508.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.248484.1, SRR1803616.79865.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264187.7/ ENSP00000264187.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGAT ATTGATGAAT GTTCAGAACA ACCCTCAGTG 
TGTGGGAGCC ACACAATCTG CAATAATCAC CCAGGAACCT TCCGCTGCGA GTGTGTGGAG 
GGCTACCAGT TTTCAGATGA GGGAACGTGT GTGGCTGTCG TGGACCAGCG CCCCATCAAC 
TACTGTGAAA CTGGCCTTCA TAACTGCGAC ATACCCCAGC GGGCCCAGTG TATCTACACA 
GGAGGCTCCT CCTACACCTG TTCCTGCTTG CCAGGCTTTT CTGGGGATGG CCAAGCCTGC 
CAAGATGTAG ATGAATGCCA GCCAAGCCGA TGTCACCCTG ACGCCTTCTG CTACAACACT 
CCAGGCTCTT TCACGTGCCA GTGCAAACCT GGTTATCAGG GAGACGGCTT CCGTTGCGTG 
CCCGGAGAGG TGGAGAAAAC CCGGTGCCAG CACGAGCGAG AACACATTCT CGGGGCAGCG 
GGGGCGACAG ACCCACAGCG ACCCATTCCT CCGGGGCTGT TCGTTCCTGA GTGCGATGCG 
CACGGGCACT ACGCGCCCAC CCAGTGCCAC GGCAGCACCG GCTACTGCTG GTGCGTGGAT 
CGCGACGGCC GCGAGGTGGA GGGCACCAGG ACCAGGCCCG GGATGACGCC CCCGTGTCTG 
AGTACAGTGG CTCCCCCGAT TCACCAAGGA CCTGCGGTGC CTACCGCCGT GATCCCCTTG 
CCTCCTGGGA CCCATTTACT CTTTGCCCAG ACTGGGAAGA TTGAGCGCCT GCCCCTGGAG 
GGAAATACCA TGAGGAAGAC AGAAGCAAAG GCGTTCCTTC ATGTCCCGGC TAAAGTCATC 
ATTGGACTGG CCTTTGACTG CGTGGACAAG ATGGTTTACT GGACGGACAT CACTGAGCCT 
TCCATTGGGA GAGCTAGTCT ACATGGTGGA GAGCCAACCA CCATCATTAG ACAAGATCTT 
GGAAGTCCAG AAGGTATCGC TGTTGATCAC CTTGGCCGCA ACATCTTCTG GACAGACTCT 
AACCTGGATC GAATAGAAGT GGCGAAGCTG GACGGCACGC AGCGCCGGGT GCTCTTTGAG 
ACTGACTTGG TGAATCCCAG AGGCATTGTA ACGGATTCCG TGAGAGGGAA CCTTTACTGG 
ACAGACTGGA ACAGAGATAA CCCCAAGATT GAAACTTCCT ACATGGACGG CACGAACCGG 
AGGATCCTTG TGCAGGATGA CCTGGGCTTG CCCAATGGAC TGACCTTCGA TGCGTTCTCA 
TCTCAGCTCT GCTGGGTGGA TGCAGGCACC AATCGGGCGG AATGCCTGAA CCCCAGTCAG 
CCCAGCAGAC GCAAGGCTCT CGAAGGGCTC CAGTATCCTT TTGCTGTGAC GAGCTACGGG 
AAGAATCTGT ATTTCACAGA CTGGAAGATG AATTCCGTGG TTGCTCTCGA TCTTGCAATT 
TCCAAGGAGA CGGATGCTTT CCAACCCCAC AAGCAGACCC GGCTGTATGG CATCACCACG 
GCCCTGTCTC AGTGTCCGCA AGGCCATAAC TACTGCTCAG TGAACAATGG CGGCTGCACC 
CACCTATGCT TGGCCACCCC AGGGAGCAGG ACCTGCCGTT GCCCTGACAA CACCTTGGGA 
GTTGACTGTA TCGAACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002499.2
CDS	12..3755
Translation	MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDLELEDGDDFVSPALELSGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESVENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVHAECRDYATGFCCSCVAGYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPIVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPVGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIPFGSSVHIEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSRPALPSTQQLSVDSVFVLYNQEEKILRYALSNSIGPVREGSPDALQNPCYIGTHGCDTNAACRPGPRTQFTCECSIGFRGDGRTCYDIDECSEQPSVCGSHTICNNHPGTFRCECVEGYQFSDEGTCVAVVDQRPINYCETGLHNCDIPQRAQCIYTGGSSYTCSCLPGFSGDGQACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCVPGEVEKTRCQHEREHILGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTRTRPGMTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNPKIETSYMDGTNRRILVQDDLGLPNGLTFDAFSSQLCWVDAGTNRAECLNPSQPSRRKALEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIEQK

Target ORF information:

RefSeq Version	NM_002508.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 1 (NID1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002508.3

ORF Insert Sequence:

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGAT ATTGATGAAT GTTCAGAACA ACCCTCAGTG 
TGTGGGAGCC ACACAATCTG CAATAATCAC CCAGGAACCT TCCGCTGCGA GTGTGTGGAG 
GGCTACCAGT TTTCAGATGA GGGAACGTGT GTGGCTGTCG TGGACCAGCG CCCCATCAAC 
TACTGTGAAA CTGGCCTTCA TAACTGCGAC ATACCCCAGC GGGCCCAGTG TATCTACACA 
GGAGGCTCCT CCTACACCTG TTCCTGCTTG CCAGGCTTTT CTGGGGATGG CCAAGCCTGC 
CAAGATGTAG ATGAATGCCA GCCAAGCCGA TGTCACCCTG ACGCCTTCTG CTACAACACT 
CCAGGCTCTT TCACGTGCCA GTGCAAACCT GGTTATCAGG GAGACGGCTT CCGTTGCGTG 
CCCGGAGAGG TGGAGAAAAC CCGGTGCCAG CACGAGCGAG AACACATTCT CGGGGCAGCG 
GGGGCGACAG ACCCACAGCG ACCCATTCCT CCGGGGCTGT TCGTTCCTGA GTGCGATGCG 
CACGGGCACT ACGCGCCCAC CCAGTGCCAC GGCAGCACCG GCTACTGCTG GTGCGTGGAT 
CGCGACGGCC GCGAGGTGGA GGGCACCAGG ACCAGGCCCG GGATGACGCC CCCGTGTCTG 
AGTACAGTGG CTCCCCCGAT TCACCAAGGA CCTGCGGTGC CTACCGCCGT GATCCCCTTG 
CCTCCTGGGA CCCATTTACT CTTTGCCCAG ACTGGGAAGA TTGAGCGCCT GCCCCTGGAG 
GGAAATACCA TGAGGAAGAC AGAAGCAAAG GCGTTCCTTC ATGTCCCGGC TAAAGTCATC 
ATTGGACTGG CCTTTGACTG CGTGGACAAG ATGGTTTACT GGACGGACAT CACTGAGCCT 
TCCATTGGGA GAGCTAGTCT ACATGGTGGA GAGCCAACCA CCATCATTAG ACAAGATCTT 
GGAAGTCCAG AAGGTATCGC TGTTGATCAC CTTGGCCGCA ACATCTTCTG GACAGACTCT 
AACCTGGATC GAATAGAAGT GGCGAAGCTG GACGGCACGC AGCGCCGGGT GCTCTTTGAG 
ACTGACTTGG TGAATCCCAG AGGCATTGTA ACGGATTCCG TGAGAGGGAA CCTTTACTGG 
ACAGACTGGA ACAGAGATAA CCCCAAGATT GAAACTTCCT ACATGGACGG CACGAACCGG 
AGGATCCTTG TGCAGGATGA CCTGGGCTTG CCCAATGGAC TGACCTTCGA TGCGTTCTCA 
TCTCAGCTCT GCTGGGTGGA TGCAGGCACC AATCGGGCGG AATGCCTGAA CCCCAGTCAG 
CCCAGCAGAC GCAAGGCTCT CGAAGGGCTC CAGTATCCTT TTGCTGTGAC GAGCTACGGG 
AAGAATCTGT ATTTCACAGA CTGGAAGATG AATTCCGTGG TTGCTCTCGA TCTTGCAATT 
TCCAAGGAGA CGGATGCTTT CCAACCCCAC AAGCAGACCC GGCTGTATGG CATCACCACG 
GCCCTGTCTC AGTGTCCGCA AGGCCATAAC TACTGCTCAG TGAACAATGG CGGCTGCACC 
CACCTATGCT TGGCCACCCC AGGGAGCAGG ACCTGCCGTT GCCCTGACAA CACCTTGGGA 
GTTGACTGTA TCGAACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID1 ( XM_011544195.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542497.1 Homo sapiens nidogen 1 (NID1), transcript variant X1, mRNA.

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CloneID	OHu59277
Clone ID Related Accession (Same CDS sequence)	XM_011544195.2 , XM_011544195.3
Accession Version	XM_011544195.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nidogen-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGCT GTCGTGGACC AGCGCCCCAT CAACTACTGT 
GAAACTGGCC TTCATAACTG CGACATACCC CAGCGGGCCC AGTGTATCTA CACAGGAGGC 
TCCTCCTACA CCTGTTCCTG CTTGCCAGGC TTTTCTGGGG ATGGCCAAGC CTGCCAAGAT 
GTAGATGAAT GCCAGCCAAG CCGATGTCAC CCTGACGCCT TCTGCTACAA CACTCCAGGC 
TCTTTCACGT GCCAGTGCAA ACCTGGTTAT CAGGGAGACG GCTTCCGTTG CGTGCCCGGA 
GAGGTGGAGA AAACCCGGTG CCAGCACGAG CGAGAACACA TTCTCGGGGC AGCGGGGGCG 
ACAGACCCAC AGCGACCCAT TCCTCCGGGG CTGTTCGTTC CTGAGTGCGA TGCGCACGGG 
CACTACGCGC CCACCCAGTG CCACGGCAGC ACCGGCTACT GCTGGTGCGT GGATCGCGAC 
GGCCGCGAGG TGGAGGGCAC CAGGACCAGG CCCGGGATGA CGCCCCCGTG TCTGAGTACA 
GTGGCTCCCC CGATTCACCA AGGACCTGCG GTGCCTACCG CCGTGATCCC CTTGCCTCCT 
GGGACCCATT TACTCTTTGC CCAGACTGGG AAGATTGAGC GCCTGCCCCT GGAGGGAAAT 
ACCATGAGGA AGACAGAAGC AAAGGCGTTC CTTCATGTCC CGGCTAAAGT CATCATTGGA 
CTGGCCTTTG ACTGCGTGGA CAAGATGGTT TACTGGACGG ACATCACTGA GCCTTCCATT 
GGGAGAGCTA GTCTACATGG TGGAGAGCCA ACCACCATCA TTAGACAAGA TCTTGGAAGT 
CCAGAAGGTA TCGCTGTTGA TCACCTTGGC CGCAACATCT TCTGGACAGA CTCTAACCTG 
GATCGAATAG AAGTGGCGAA GCTGGACGGC ACGCAGCGCC GGGTGCTCTT TGAGACTGAC 
TTGGTGAATC CCAGAGGCAT TGTAACGGAT TCCGTGAGAG GGAACCTTTA CTGGACAGAC 
TGGAACAGAG ATAACCCCAA GATTGAAACT TCCTACATGG ACGGCACGAA CCGGAGGATC 
CTTGTGCAGG ATGACCTGGG CTTGCCCAAT GGACTGACCT TCGATGCGTT CTCATCTCAG 
CTCTGCTGGG TGGATGCAGG CACCAATCGG GCGGAATGCC TGAACCCCAG TCAGCCCAGC 
AGACGCAAGG CTCTCGAAGG GCTCCAGTAT CCTTTTGCTG TGACGAGCTA CGGGAAGAAT 
CTGTATTTCA CAGACTGGAA GATGAATTCC GTGGTTGCTC TCGATCTTGC AATTTCCAAG 
GAGACGGATG CTTTCCAACC CCACAAGCAG ACCCGGCTGT ATGGCATCAC CACGGCCCTG 
TCTCAGTGTC CGCAAGGCCA TAACTACTGC TCAGTGAACA ATGGCGGCTG CACCCACCTA 
TGCTTGGCCA CCCCAGGGAG CAGGACCTGC CGTTGCCCTG ACAACACCTT GGGAGTTGAC 
TGTATCGAAC AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542497.1
CDS	100..3717
Translation	MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDLELEDGDDFVSPALELSGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESVENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVHAECRDYATGFCCSCVAGYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPIVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPVGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIPFGSSVHIEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSRPALPSTQQLSVDSVFVLYNQEEKILRYALSNSIGPVREGSPDALQNPCYIGTHGCDTNAACRPGPRTQFTCECSIGFRGDGRTCYAVVDQRPINYCETGLHNCDIPQRAQCIYTGGSSYTCSCLPGFSGDGQACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCVPGEVEKTRCQHEREHILGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTRTRPGMTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNPKIETSYMDGTNRRILVQDDLGLPNGLTFDAFSSQLCWVDAGTNRAECLNPSQPSRRKALEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIEQK

Target ORF information:

RefSeq Version	XM_011544195.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 1 (NID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544195.2

ORF Insert Sequence:

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGCT GTCGTGGACC AGCGCCCCAT CAACTACTGT 
GAAACTGGCC TTCATAACTG CGACATACCC CAGCGGGCCC AGTGTATCTA CACAGGAGGC 
TCCTCCTACA CCTGTTCCTG CTTGCCAGGC TTTTCTGGGG ATGGCCAAGC CTGCCAAGAT 
GTAGATGAAT GCCAGCCAAG CCGATGTCAC CCTGACGCCT TCTGCTACAA CACTCCAGGC 
TCTTTCACGT GCCAGTGCAA ACCTGGTTAT CAGGGAGACG GCTTCCGTTG CGTGCCCGGA 
GAGGTGGAGA AAACCCGGTG CCAGCACGAG CGAGAACACA TTCTCGGGGC AGCGGGGGCG 
ACAGACCCAC AGCGACCCAT TCCTCCGGGG CTGTTCGTTC CTGAGTGCGA TGCGCACGGG 
CACTACGCGC CCACCCAGTG CCACGGCAGC ACCGGCTACT GCTGGTGCGT GGATCGCGAC 
GGCCGCGAGG TGGAGGGCAC CAGGACCAGG CCCGGGATGA CGCCCCCGTG TCTGAGTACA 
GTGGCTCCCC CGATTCACCA AGGACCTGCG GTGCCTACCG CCGTGATCCC CTTGCCTCCT 
GGGACCCATT TACTCTTTGC CCAGACTGGG AAGATTGAGC GCCTGCCCCT GGAGGGAAAT 
ACCATGAGGA AGACAGAAGC AAAGGCGTTC CTTCATGTCC CGGCTAAAGT CATCATTGGA 
CTGGCCTTTG ACTGCGTGGA CAAGATGGTT TACTGGACGG ACATCACTGA GCCTTCCATT 
GGGAGAGCTA GTCTACATGG TGGAGAGCCA ACCACCATCA TTAGACAAGA TCTTGGAAGT 
CCAGAAGGTA TCGCTGTTGA TCACCTTGGC CGCAACATCT TCTGGACAGA CTCTAACCTG 
GATCGAATAG AAGTGGCGAA GCTGGACGGC ACGCAGCGCC GGGTGCTCTT TGAGACTGAC 
TTGGTGAATC CCAGAGGCAT TGTAACGGAT TCCGTGAGAG GGAACCTTTA CTGGACAGAC 
TGGAACAGAG ATAACCCCAA GATTGAAACT TCCTACATGG ACGGCACGAA CCGGAGGATC 
CTTGTGCAGG ATGACCTGGG CTTGCCCAAT GGACTGACCT TCGATGCGTT CTCATCTCAG 
CTCTGCTGGG TGGATGCAGG CACCAATCGG GCGGAATGCC TGAACCCCAG TCAGCCCAGC 
AGACGCAAGG CTCTCGAAGG GCTCCAGTAT CCTTTTGCTG TGACGAGCTA CGGGAAGAAT 
CTGTATTTCA CAGACTGGAA GATGAATTCC GTGGTTGCTC TCGATCTTGC AATTTCCAAG 
GAGACGGATG CTTTCCAACC CCACAAGCAG ACCCGGCTGT ATGGCATCAC CACGGCCCTG 
TCTCAGTGTC CGCAAGGCCA TAACTACTGC TCAGTGAACA ATGGCGGCTG CACCCACCTA 
TGCTTGGCCA CCCCAGGGAG CAGGACCTGC CGTTGCCCTG ACAACACCTT GGGAGTTGAC 
TGTATCGAAC AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NID1 ( XM_011544195.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542497.1 Homo sapiens nidogen 1 (NID1), transcript variant X1, mRNA.

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CloneID	OHu59277
Clone ID Related Accession (Same CDS sequence)	XM_011544195.2 , XM_011544195.3
Accession Version	XM_011544195.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nidogen-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544195.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGCT GTCGTGGACC AGCGCCCCAT CAACTACTGT 
GAAACTGGCC TTCATAACTG CGACATACCC CAGCGGGCCC AGTGTATCTA CACAGGAGGC 
TCCTCCTACA CCTGTTCCTG CTTGCCAGGC TTTTCTGGGG ATGGCCAAGC CTGCCAAGAT 
GTAGATGAAT GCCAGCCAAG CCGATGTCAC CCTGACGCCT TCTGCTACAA CACTCCAGGC 
TCTTTCACGT GCCAGTGCAA ACCTGGTTAT CAGGGAGACG GCTTCCGTTG CGTGCCCGGA 
GAGGTGGAGA AAACCCGGTG CCAGCACGAG CGAGAACACA TTCTCGGGGC AGCGGGGGCG 
ACAGACCCAC AGCGACCCAT TCCTCCGGGG CTGTTCGTTC CTGAGTGCGA TGCGCACGGG 
CACTACGCGC CCACCCAGTG CCACGGCAGC ACCGGCTACT GCTGGTGCGT GGATCGCGAC 
GGCCGCGAGG TGGAGGGCAC CAGGACCAGG CCCGGGATGA CGCCCCCGTG TCTGAGTACA 
GTGGCTCCCC CGATTCACCA AGGACCTGCG GTGCCTACCG CCGTGATCCC CTTGCCTCCT 
GGGACCCATT TACTCTTTGC CCAGACTGGG AAGATTGAGC GCCTGCCCCT GGAGGGAAAT 
ACCATGAGGA AGACAGAAGC AAAGGCGTTC CTTCATGTCC CGGCTAAAGT CATCATTGGA 
CTGGCCTTTG ACTGCGTGGA CAAGATGGTT TACTGGACGG ACATCACTGA GCCTTCCATT 
GGGAGAGCTA GTCTACATGG TGGAGAGCCA ACCACCATCA TTAGACAAGA TCTTGGAAGT 
CCAGAAGGTA TCGCTGTTGA TCACCTTGGC CGCAACATCT TCTGGACAGA CTCTAACCTG 
GATCGAATAG AAGTGGCGAA GCTGGACGGC ACGCAGCGCC GGGTGCTCTT TGAGACTGAC 
TTGGTGAATC CCAGAGGCAT TGTAACGGAT TCCGTGAGAG GGAACCTTTA CTGGACAGAC 
TGGAACAGAG ATAACCCCAA GATTGAAACT TCCTACATGG ACGGCACGAA CCGGAGGATC 
CTTGTGCAGG ATGACCTGGG CTTGCCCAAT GGACTGACCT TCGATGCGTT CTCATCTCAG 
CTCTGCTGGG TGGATGCAGG CACCAATCGG GCGGAATGCC TGAACCCCAG TCAGCCCAGC 
AGACGCAAGG CTCTCGAAGG GCTCCAGTAT CCTTTTGCTG TGACGAGCTA CGGGAAGAAT 
CTGTATTTCA CAGACTGGAA GATGAATTCC GTGGTTGCTC TCGATCTTGC AATTTCCAAG 
GAGACGGATG CTTTCCAACC CCACAAGCAG ACCCGGCTGT ATGGCATCAC CACGGCCCTG 
TCTCAGTGTC CGCAAGGCCA TAACTACTGC TCAGTGAACA ATGGCGGCTG CACCCACCTA 
TGCTTGGCCA CCCCAGGGAG CAGGACCTGC CGTTGCCCTG ACAACACCTT GGGAGTTGAC 
TGTATCGAAC AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542497.1
CDS	14..3631
Translation	MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDLELEDGDDFVSPALELSGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESVENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVHAECRDYATGFCCSCVAGYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPIVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPVGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIPFGSSVHIEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSRPALPSTQQLSVDSVFVLYNQEEKILRYALSNSIGPVREGSPDALQNPCYIGTHGCDTNAACRPGPRTQFTCECSIGFRGDGRTCYAVVDQRPINYCETGLHNCDIPQRAQCIYTGGSSYTCSCLPGFSGDGQACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCVPGEVEKTRCQHEREHILGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTRTRPGMTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNPKIETSYMDGTNRRILVQDDLGLPNGLTFDAFSSQLCWVDAGTNRAECLNPSQPSRRKALEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIEQK

Target ORF information:

RefSeq Version	XM_011544195.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nidogen 1 (NID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544195.3

ORF Insert Sequence:

ATGTTGGCCT CGAGCAGCCG GATCCGGGCT GCGTGGACGC GGGCGCTGCT GCTGCCGCTG 
CTGCTGGCGG GGCCTGTGGG CTGCCTGAGC CGCCAGGAGC TCTTTCCCTT CGGCCCCGGA 
CAGGGGGACC TGGAGCTGGA GGACGGGGAT GACTTCGTCT CTCCTGCCCT GGAGCTGAGT 
GGGGCGCTCC GCTTCTACGA CAGATCCGAC ATCGACGCAG TCTACGTCAC CACAAATGGC 
ATCATTGCTA CGAGTGAACC CCCGGCCAAA GAATCCCATC CCGGGCTCTT CCCACCAACA 
TTCGGTGCAG TCGCCCCTTT CCTGGCGGAC TTGGACACGA CCGATGGCCT GGGGAAGGTT 
TATTATCGAG AAGACTTATC CCCCTCCATC ACTCAGCGAG CAGCAGAGTG TGTCCACAGA 
GGGTTCCCGG AGATCTCTTT CCAGCCTAGT AGCGCGGTGG TTGTCACTTG GGAATCCGTG 
GCCCCCTACC AAGGGCCCAG CAGGGACCCA GACCAGAAAG GCAAGAGAAA CACGTTCCAG 
GCTGTTCTAG CCTCCTCTGA TTCCAGCTCC TATGCCATTT TCCTTTATCC TGAGGATGGT 
CTGCAGTTCC ATACGACATT CTCAAAGAAG GAAAACAACC AAGTTCCTGC CGTGGTTGCA 
TTCAGTCAAG GTTCAGTGGG ATTCTTATGG AAGAGCAACG GAGCTTATAA CATATTTGCT 
AATGACAGGG AATCAGTTGA AAATTTGGCC AAGAGTAGTA ACTCTGGGCA GCAGGGTGTC 
TGGGTGTTTG AGATTGGGAG TCCAGCCACC ACCAATGGCG TGGTGCCTGC AGACGTGATC 
CTCGGAACTG AAGATGGGGC AGAGTATGAT GATGAGGATG AAGATTATGA CCTGGCGACC 
ACTCGTCTGG GCCTGGAGGA TGTGGGCACC ACGCCCTTCT CCTACAAGGC TCTGAGAAGG 
GGAGGTGCTG ACACATACAG TGTGCCCAGC GTCCTCTCCC CGCGCCGGGC AGCTACCGAA 
AGGCCCCTTG GACCTCCCAC AGAGAGAACC AGGTCTTTCC AGTTGGCAGT GGAGACTTTT 
CACCAGCAGC ACCCTCAGGT CATAGATGTG GATGAAGTTG AGGAAACAGG AGTTGTTTTC 
AGCTATAACA CGGATTCCCG CCAGACGTGT GCTAACAACA GACACCAGTG CTCGGTGCAC 
GCAGAGTGCA GGGACTACGC CACGGGCTTC TGCTGCAGCT GTGTCGCTGG CTATACGGGC 
AATGGCAGGC AATGTGTTGC AGAAGGTTCC CCCCAGCGAG TCAATGGCAA GGTGAAAGGA 
AGGATCTTTG TGGGGAGCAG CCAGGTCCCC ATTGTCTTTG AGAACACTGA CCTCCACTCT 
TACGTAGTAA TGAACCACGG GCGCTCCTAC ACAGCCATCA GCACCATTCC CGAGACCGTT 
GGATATTCTC TGCTTCCACT GGCCCCAGTT GGAGGCATCA TTGGATGGAT GTTTGCAGTG 
GAGCAGGACG GATTCAAGAA TGGGTTCAGC ATCACCGGGG GTGAGTTCAC TCGCCAGGCT 
GAGGTGACCT TCGTGGGGCA CCCGGGCAAT CTGGTCATTA AGCAGCGGTT CAGCGGCATC 
GATGAGCATG GGCACCTGAC CATCGACACG GAGCTGGAGG GCCGCGTGCC GCAGATTCCG 
TTCGGCTCCT CCGTGCACAT TGAGCCCTAC ACGGAGCTGT ACCACTACTC CACCTCAGTG 
ATCACTTCCT CCTCCACCCG GGAGTACACG GTGACTGAGC CCGAGCGAGA TGGGGCATCT 
CCTTCACGCA TCTACACTTA CCAGTGGCGC CAGACCATCA CCTTCCAGGA ATGCGTCCAC 
GATGACTCCC GGCCAGCCCT GCCCAGCACC CAGCAGCTCT CGGTGGACAG CGTGTTCGTC 
CTGTACAACC AGGAGGAGAA GATCTTGCGC TATGCTCTCA GCAACTCCAT TGGGCCTGTG 
AGGGAAGGCT CCCCTGATGC TCTTCAGAAT CCCTGCTACA TCGGCACTCA TGGGTGTGAC 
ACCAACGCGG CCTGTCGCCC TGGTCCCAGG ACACAGTTCA CCTGCGAGTG CTCCATCGGC 
TTCCGAGGAG ACGGGCGAAC CTGCTATGCT GTCGTGGACC AGCGCCCCAT CAACTACTGT 
GAAACTGGCC TTCATAACTG CGACATACCC CAGCGGGCCC AGTGTATCTA CACAGGAGGC 
TCCTCCTACA CCTGTTCCTG CTTGCCAGGC TTTTCTGGGG ATGGCCAAGC CTGCCAAGAT 
GTAGATGAAT GCCAGCCAAG CCGATGTCAC CCTGACGCCT TCTGCTACAA CACTCCAGGC 
TCTTTCACGT GCCAGTGCAA ACCTGGTTAT CAGGGAGACG GCTTCCGTTG CGTGCCCGGA 
GAGGTGGAGA AAACCCGGTG CCAGCACGAG CGAGAACACA TTCTCGGGGC AGCGGGGGCG 
ACAGACCCAC AGCGACCCAT TCCTCCGGGG CTGTTCGTTC CTGAGTGCGA TGCGCACGGG 
CACTACGCGC CCACCCAGTG CCACGGCAGC ACCGGCTACT GCTGGTGCGT GGATCGCGAC 
GGCCGCGAGG TGGAGGGCAC CAGGACCAGG CCCGGGATGA CGCCCCCGTG TCTGAGTACA 
GTGGCTCCCC CGATTCACCA AGGACCTGCG GTGCCTACCG CCGTGATCCC CTTGCCTCCT 
GGGACCCATT TACTCTTTGC CCAGACTGGG AAGATTGAGC GCCTGCCCCT GGAGGGAAAT 
ACCATGAGGA AGACAGAAGC AAAGGCGTTC CTTCATGTCC CGGCTAAAGT CATCATTGGA 
CTGGCCTTTG ACTGCGTGGA CAAGATGGTT TACTGGACGG ACATCACTGA GCCTTCCATT 
GGGAGAGCTA GTCTACATGG TGGAGAGCCA ACCACCATCA TTAGACAAGA TCTTGGAAGT 
CCAGAAGGTA TCGCTGTTGA TCACCTTGGC CGCAACATCT TCTGGACAGA CTCTAACCTG 
GATCGAATAG AAGTGGCGAA GCTGGACGGC ACGCAGCGCC GGGTGCTCTT TGAGACTGAC 
TTGGTGAATC CCAGAGGCAT TGTAACGGAT TCCGTGAGAG GGAACCTTTA CTGGACAGAC 
TGGAACAGAG ATAACCCCAA GATTGAAACT TCCTACATGG ACGGCACGAA CCGGAGGATC 
CTTGTGCAGG ATGACCTGGG CTTGCCCAAT GGACTGACCT TCGATGCGTT CTCATCTCAG 
CTCTGCTGGG TGGATGCAGG CACCAATCGG GCGGAATGCC TGAACCCCAG TCAGCCCAGC 
AGACGCAAGG CTCTCGAAGG GCTCCAGTAT CCTTTTGCTG TGACGAGCTA CGGGAAGAAT 
CTGTATTTCA CAGACTGGAA GATGAATTCC GTGGTTGCTC TCGATCTTGC AATTTCCAAG 
GAGACGGATG CTTTCCAACC CCACAAGCAG ACCCGGCTGT ATGGCATCAC CACGGCCCTG 
TCTCAGTGTC CGCAAGGCCA TAACTACTGC TCAGTGAACA ATGGCGGCTG CACCCACCTA 
TGCTTGGCCA CCCCAGGGAG CAGGACCTGC CGTTGCCCTG ACAACACCTT GGGAGTTGAC 
TGTATCGAAC AGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Identification of Nidogen 1 as a lung metastasis protein through secretome analysis.
Genes & development31(14)1439-1455(2017 Jul)
Ale?kovi? M,Wei Y,LeRoy G,Sidoli S,Liu DD,Garcia BA,Kang Y

NID1, a new regulator of EMT required for metastasis and chemoresistance of ovarian cancer cells.
Oncotarget8(20)33110-33121(2017 May)
Zhou Y,Zhu Y,Fan X,Zhang C,Wang Y,Zhang L,Zhang H,Wen T,Zhang K,Huo X,Jiang X,Bu Y,Zhang Y

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NID1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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