LOC100491930 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100491930
Entrez Gene ID	100491930
Full Name	transmembrane protein 2-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012959543.2	XP_012814997.1	transmembrane protein 2-like isoform X2
XM_012959545.2	XP_012814999.1	transmembrane protein 2-like isoform X3
XM_012959543.3	XP_012814997.2	cell surface hyaluronidase isoform X2
XM_031899223.1	XP_031755083.1	cell surface hyaluronidase isoform X3
XM_002932255.4	XP_002932301.3	transmembrane protein 2-like isoform X1
XM_002932255.5	XP_002932301.4	cell surface hyaluronidase isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TMEM2	XP_413852.4
Xenopus tropicalis (tropical clawed frog)	LOC100491930	XP_002932301.2

Gene Ontology

The following LOC100491930 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100491930 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35213	XM_012959545.2 Latest version!	Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa35210	XM_002932255.4 Latest version!	Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa35211	XM_012959543.2 Latest version!	Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa55177	XM_002932255.5 Latest version!	Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa55178	XM_012959543.3 Latest version!	Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa55179	XM_031899223.1 Latest version!	Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100491930 ( XM_012959545.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814999.1 Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa35213
Clone ID Related Accession (Same CDS sequence)	XM_012959545.2
Accession Version	XM_012959545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane protein 2-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTGAGAGAGG TCTAAACATG 
CTCCCAACAG CCAATATGGG ATGCTGTGCA CATGGACTCT TGTCTCCTTC CTCTGTAGAT 
GAAGACTGTC TGCAGATCCA CTTACACAGG CAATGGCACG GGGACACTGC TGACACTCAT 
AATTGTGACT CATGCCCAGC ACCTACTTAT CAACCAGGAG AAAAGTGGAG CAGACACCAA 
GACATTCTCA CTGTCACCTA TCTCTCCGGG AGAGGAAACA TACCTATGGG AAAACTTGCA 
GCCTTTCTGC CTTCATCACC CAGCCTTTCA CAAGTGTTCC TATTCCTTCT GATGCTTCTG 
TGCTGGCAGA GATGTGCAGC TGGAAATCTA AACTTCTTTC CAGAAGACAG CATCAATGCC 
AACATGTCTG TAGGAAACCT GGGTCCTGAT GAGTTGAGTG TGGTTAGAAA TGAAGCTCCA 
TCACTTTCCT ATGTCCATGG GGGAAATGCA TTGACTGAAG GAGACCCAGT GTTTGCACCA 
AGATACAACA TAACTCTGCA CAAAAAGGGC AAGCTATGGT CATGTGACAG TCTTCTTAAG 
CTCACTAGAC ATATTCTATT GACTATGTTG GGGCCTAGTT TGGCTTTTGA AGTCTTCCCA 
TATCATCACT GTAACCAGGA CTTAAAGCCA AGGGACACAC TTATTAGTCC ACTGAGGCTG 
TTACACGCGC ACGTGAAACC ACTTCTACTT CACAGAGTTT TAATATTCCT GGGGGAGCTC 
GGATTTTTGG TTGAATCCCA ACAAGCTAAA GCCCTGTCGG TACTGAAGAA GCAGATCTTC 
AAAACAGGAA AACCAATGAA GGAGGCTGGA GTGAAACCAA AGGACAATTG CCCAGATAAG 
AAGCCACATC TGATCCCTTG GAACCCAGGA CATGATGGAA GCAGATCAGC TGTTATCGGT 
TATGGTGTTG CCCTACGATT GGAGTCATCA GCCACTTTCC ACTCACTGCT TATTCAGAAT 
GGAGGATCTT TAGTGTTTGC AGATGACCTA GTGCCAATTA CTTTGAGAGC ACAGTATATT 
TTAATCAGAA ATGGCGGATC TTTTCACATT GGCTCAGAAA AGTGTCCTTA TAGGTCCAAT 
GCTACCATCA CGCTTTATGG GAAGTCTACA GAAGGTGACA ATGTAGAAGA CTTTGGAAAG 
AAGTTCTTAG GAGTAGATAC AGGAGGGATT TTGGAATTAC ATGGAAGGAA ACCATTATCA 
TGGACTTTGT TAGATGCAAC CTTACATCCA GGAGGGCTCC CCCATGGACC ATATAAGTGG 
GAGAAGCAAT GGGGCAGCAG GGGCATTAAC ATACGAATCA TTGACCCTGA AACTGGTCAA 
GTAATAGCTG CTGATCGCTT TGACACCCAC ATGTTTCTGG ATGAAGGGAA AAGGCTGGAG 
GAGTTCCTTG CTCAACAGAG TCCAGGCAAG ATAGTGGCAG CAGCAGTGGG TGACTCAGCA 
GCCAAGAGCT TAACCCCAGA CACAAGGAAA TTTCTAAGAG ATGCTTTGAG CAGCAAATAT 
ATCTTTCACT TGGGCTACAG GCAGCCCTGG GCTTTAGTTG GGGTGCTTGG AGAAGACCTG 
TTGATGGTAG CAGAGGACAG AAAGCAATAT GAGAGCAATG GGACCACTGG TCTTGCAACT 
GCCACAAGGG AATTCCAGAC ATATTCGGGG CTGCACTTCA CTGTAACTGC TTACAGTGGC 
TGGATTAAAG GAGTTCCGCG AAATGGATTC AGAGTGGCAG TGTCGAGGGG CATCATCCTC 
ACATTACTGG ATGATGTTAC AAGTTGGTTG CCTGGTAACA GAATAGTCGT TGCTAGTACC 
GATTACTCAA TGCATCAGAC TGAAGAATTC ACCCTGCTTC CCTGCCCTGA ATGCAGAAGT 
AATCAAGTCA AAATTAGCGG TAGCCCACTT TATTTGCATA TTGGTGAAGT TATTGATGGA 
ATTGACATGA GAGCAGAGGT TGGGGTTCTC ACAAGAAATG TTCTTATCCA TGGTGAAATG 
GAAGATTTAT GTTATGGACA AAACCAGTGT CAGTTTTTCA ACTATGATAC CTTTGGAGGA 
CAAGTCAAGA TTCAAGGAAG ATTTGCATCA GTTCATCTGT CAGGAGTAGA ACTAAAAAAC 
ATGGGGCAGC AAATCTTGGG CAGTTACCCA GTTCATTTCC ACATGACAGG AACTGTAGAT 
GAGGAAGGGG GCTATAACCC TCCAACGTAT GTGGACAATC TTTCCATCCA CCACTGTTTC 
TCGAGATGTG TGGTGATTCA TGGGACCCAT GGACTTCTGG TGAAAGACAC GATTGGCTAT 
GACACACTGG GGCACTGTTT TTTTCTTGAG GATGGTGTTG AACAAAGAAA TACATTTTAT 
CACAATCTAG GATTGCTGAC AAAGGCTGGG ACCATCTTGC CCACAGACCG AAGTGAAGCC 
ATGTGTCTTG CTATCCGAGA TTCTGTCTAT GGAGATTATG TTCCTGTGCC TAGCATTGAC 
TGCATGGCTG TCAGCACATT TTGGATTGCA AACCCTAATA ACAATTTAAT AGGAAATGCT 
GCAGGAGGGG CACAGGATGT TGGCATATGG TTCATATTCC ACCGAGTTCC CTCTGGCCTT 
TGTGAAGGAC AGTATGCTGA AGGACATTCA GAATTTACCC CATTGGGGGT GTTTTACAAC 
AACAGGGTAC ACTCCAACTT CAAGGCCGGA TTATTCATTG GCAAAGGAGT CAAAACCACA 
GTAGCAAATG CTGAGAATCC AAGGGAATAT CTGACAGTAG ATAATGCCCG GTTTCGTCCC 
CATCAAGATT CAGATCCCAC CAAGCCTCGA ATACCAGCTG TTATTGATGG CCTAATAGCT 
TTCAAAAACA ATGACCATGG TGCATGGGCT AGAGGAGGGG ACATTATTTT CCGTAACTCA 
GGGTTTTCTG ATAATGGCAT TGGACTAACT TTAGCCAGTG ATGGGACATT TCCTACTGAT 
GAAGGCTCTA GCTTGGAGAT CACAGACTCC ATATTTGTGG GTGAGAGCGA GAATGTGGGA 
TCACAAGGTG GACAGAACAT CTACTGGGGA CGTGGGGGTT TGGGGGAGCA GCGATCATTT 
CCAAGAAACA AGACATTCCC AGTTCGTGGT TTCCAAATAT ACGATGGGCC CGTCAGGCTC 
CTGAAGTGCA CTTTCAAGAA ATTTCTCCCA ACTCTTGACA GACCTGTGAG CGCTATTGGG 
TTCTCCTTGA AAAATTCTTG GCAGATAACT CCACAGAACA ATGTGTCCCT GGTCAGGATG 
GAATGGAATG TTGGGCTGAA GGTTTTCTTT GGTCATTCAG GACAATGGTT TGGCTCAAAT 
AACTTTGATG GTGATAAAGT GTCAATCTTC CATGATTTGG ATGGCTCAGT GACTGGATAT 
CCTGATACCT TTATAGGAAG GGCAGATAAT TACCTGATCC GTCACCCTGG ATGCCTCACT 
GTGCCACGGT GGAACGGAGT GATTTGTTCA GGCAGGTATG CCCAGCTTTA CATCCAGGCC 
AGACGCCCTG AGAATCTGAA CATTTTGGTC TCTAGAGCAA CTTATCCCTC TGTTCCTTTA 
AAACTTTCTG GCGTTAACCG GGGTGCACCA TATCAACAGT ACCAACCAGT GGTCATGCTG 
CATCAATCCT ATATTATCAG ATGGGAAGGT CAGTCTCCTG GAGAGGTCAT CCTATACCCC 
ATCAACTTTA ACAGGGGAGA CTGGATTCAG ATTGCCTTGT GTTATCCCAG AAGTGCACAG 
TTCCAGGTCA TGTCAGATGT TTATGATCGT CACAGTGGAA GGATACACAG CGTAAGACAC 
TATTCCTGGG CTCATACCAT CAGCCAGGTT GTTAATCAGT CAGAGACAAG ACTTTTTCAT 
TTCAGTCAGG AATCTGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814999.1
CDS	321..4241
Translation	MCLDRRQRRSKVTGERGLNMLPTANMGCCAHGLLSPSSVDEDCLQIHLHRQWHGDTADTHNCDSCPAPTYQPGEKWSRHQDILTVTYLSGRGNIPMGKLAAFLPSSPSLSQVFLFLLMLLCWQRCAAGNLNFFPEDSINANMSVGNLGPDELSVVRNEAPSLSYVHGGNALTEGDPVFAPRYNITLHKKGKLWSCDSLLKLTRHILLTMLGPSLAFEVFPYHHCNQDLKPRDTLISPLRLLHAHVKPLLLHRVLIFLGELGFLVESQQAKALSVLKKQIFKTGKPMKEAGVKPKDNCPDKKPHLIPWNPGHDGSRSAVIGYGVALRLESSATFHSLLIQNGGSLVFADDLVPITLRAQYILIRNGGSFHIGSEKCPYRSNATITLYGKSTEGDNVEDFGKKFLGVDTGGILELHGRKPLSWTLLDATLHPGGLPHGPYKWEKQWGSRGINIRIIDPETGQVIAADRFDTHMFLDEGKRLEEFLAQQSPGKIVAAAVGDSAAKSLTPDTRKFLRDALSSKYIFHLGYRQPWALVGVLGEDLLMVAEDRKQYESNGTTGLATATREFQTYSGLHFTVTAYSGWIKGVPRNGFRVAVSRGIILTLLDDVTSWLPGNRIVVASTDYSMHQTEEFTLLPCPECRSNQVKISGSPLYLHIGEVIDGIDMRAEVGVLTRNVLIHGEMEDLCYGQNQCQFFNYDTFGGQVKIQGRFASVHLSGVELKNMGQQILGSYPVHFHMTGTVDEEGGYNPPTYVDNLSIHHCFSRCVVIHGTHGLLVKDTIGYDTLGHCFFLEDGVEQRNTFYHNLGLLTKAGTILPTDRSEAMCLAIRDSVYGDYVPVPSIDCMAVSTFWIANPNNNLIGNAAGGAQDVGIWFIFHRVPSGLCEGQYAEGHSEFTPLGVFYNNRVHSNFKAGLFIGKGVKTTVANAENPREYLTVDNARFRPHQDSDPTKPRIPAVIDGLIAFKNNDHGAWARGGDIIFRNSGFSDNGIGLTLASDGTFPTDEGSSLEITDSIFVGESENVGSQGGQNIYWGRGGLGEQRSFPRNKTFPVRGFQIYDGPVRLLKCTFKKFLPTLDRPVSAIGFSLKNSWQITPQNNVSLVRMEWNVGLKVFFGHSGQWFGSNNFDGDKVSIFHDLDGSVTGYPDTFIGRADNYLIRHPGCLTVPRWNGVICSGRYAQLYIQARRPENLNILVSRATYPSVPLKLSGVNRGAPYQQYQPVVMLHQSYIIRWEGQSPGEVILYPINFNRGDWIQIALCYPRSAQFQVMSDVYDRHSGRIHSVRHYSWAHTISQVVNQSETRLFHFSQESG

Target ORF information:

RefSeq Version	XM_012959545.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959545.2

ORF Insert Sequence:

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTGAGAGAGG TCTAAACATG 
CTCCCAACAG CCAATATGGG ATGCTGTGCA CATGGACTCT TGTCTCCTTC CTCTGTAGAT 
GAAGACTGTC TGCAGATCCA CTTACACAGG CAATGGCACG GGGACACTGC TGACACTCAT 
AATTGTGACT CATGCCCAGC ACCTACTTAT CAACCAGGAG AAAAGTGGAG CAGACACCAA 
GACATTCTCA CTGTCACCTA TCTCTCCGGG AGAGGAAACA TACCTATGGG AAAACTTGCA 
GCCTTTCTGC CTTCATCACC CAGCCTTTCA CAAGTGTTCC TATTCCTTCT GATGCTTCTG 
TGCTGGCAGA GATGTGCAGC TGGAAATCTA AACTTCTTTC CAGAAGACAG CATCAATGCC 
AACATGTCTG TAGGAAACCT GGGTCCTGAT GAGTTGAGTG TGGTTAGAAA TGAAGCTCCA 
TCACTTTCCT ATGTCCATGG GGGAAATGCA TTGACTGAAG GAGACCCAGT GTTTGCACCA 
AGATACAACA TAACTCTGCA CAAAAAGGGC AAGCTATGGT CATGTGACAG TCTTCTTAAG 
CTCACTAGAC ATATTCTATT GACTATGTTG GGGCCTAGTT TGGCTTTTGA AGTCTTCCCA 
TATCATCACT GTAACCAGGA CTTAAAGCCA AGGGACACAC TTATTAGTCC ACTGAGGCTG 
TTACACGCGC ACGTGAAACC ACTTCTACTT CACAGAGTTT TAATATTCCT GGGGGAGCTC 
GGATTTTTGG TTGAATCCCA ACAAGCTAAA GCCCTGTCGG TACTGAAGAA GCAGATCTTC 
AAAACAGGAA AACCAATGAA GGAGGCTGGA GTGAAACCAA AGGACAATTG CCCAGATAAG 
AAGCCACATC TGATCCCTTG GAACCCAGGA CATGATGGAA GCAGATCAGC TGTTATCGGT 
TATGGTGTTG CCCTACGATT GGAGTCATCA GCCACTTTCC ACTCACTGCT TATTCAGAAT 
GGAGGATCTT TAGTGTTTGC AGATGACCTA GTGCCAATTA CTTTGAGAGC ACAGTATATT 
TTAATCAGAA ATGGCGGATC TTTTCACATT GGCTCAGAAA AGTGTCCTTA TAGGTCCAAT 
GCTACCATCA CGCTTTATGG GAAGTCTACA GAAGGTGACA ATGTAGAAGA CTTTGGAAAG 
AAGTTCTTAG GAGTAGATAC AGGAGGGATT TTGGAATTAC ATGGAAGGAA ACCATTATCA 
TGGACTTTGT TAGATGCAAC CTTACATCCA GGAGGGCTCC CCCATGGACC ATATAAGTGG 
GAGAAGCAAT GGGGCAGCAG GGGCATTAAC ATACGAATCA TTGACCCTGA AACTGGTCAA 
GTAATAGCTG CTGATCGCTT TGACACCCAC ATGTTTCTGG ATGAAGGGAA AAGGCTGGAG 
GAGTTCCTTG CTCAACAGAG TCCAGGCAAG ATAGTGGCAG CAGCAGTGGG TGACTCAGCA 
GCCAAGAGCT TAACCCCAGA CACAAGGAAA TTTCTAAGAG ATGCTTTGAG CAGCAAATAT 
ATCTTTCACT TGGGCTACAG GCAGCCCTGG GCTTTAGTTG GGGTGCTTGG AGAAGACCTG 
TTGATGGTAG CAGAGGACAG AAAGCAATAT GAGAGCAATG GGACCACTGG TCTTGCAACT 
GCCACAAGGG AATTCCAGAC ATATTCGGGG CTGCACTTCA CTGTAACTGC TTACAGTGGC 
TGGATTAAAG GAGTTCCGCG AAATGGATTC AGAGTGGCAG TGTCGAGGGG CATCATCCTC 
ACATTACTGG ATGATGTTAC AAGTTGGTTG CCTGGTAACA GAATAGTCGT TGCTAGTACC 
GATTACTCAA TGCATCAGAC TGAAGAATTC ACCCTGCTTC CCTGCCCTGA ATGCAGAAGT 
AATCAAGTCA AAATTAGCGG TAGCCCACTT TATTTGCATA TTGGTGAAGT TATTGATGGA 
ATTGACATGA GAGCAGAGGT TGGGGTTCTC ACAAGAAATG TTCTTATCCA TGGTGAAATG 
GAAGATTTAT GTTATGGACA AAACCAGTGT CAGTTTTTCA ACTATGATAC CTTTGGAGGA 
CAAGTCAAGA TTCAAGGAAG ATTTGCATCA GTTCATCTGT CAGGAGTAGA ACTAAAAAAC 
ATGGGGCAGC AAATCTTGGG CAGTTACCCA GTTCATTTCC ACATGACAGG AACTGTAGAT 
GAGGAAGGGG GCTATAACCC TCCAACGTAT GTGGACAATC TTTCCATCCA CCACTGTTTC 
TCGAGATGTG TGGTGATTCA TGGGACCCAT GGACTTCTGG TGAAAGACAC GATTGGCTAT 
GACACACTGG GGCACTGTTT TTTTCTTGAG GATGGTGTTG AACAAAGAAA TACATTTTAT 
CACAATCTAG GATTGCTGAC AAAGGCTGGG ACCATCTTGC CCACAGACCG AAGTGAAGCC 
ATGTGTCTTG CTATCCGAGA TTCTGTCTAT GGAGATTATG TTCCTGTGCC TAGCATTGAC 
TGCATGGCTG TCAGCACATT TTGGATTGCA AACCCTAATA ACAATTTAAT AGGAAATGCT 
GCAGGAGGGG CACAGGATGT TGGCATATGG TTCATATTCC ACCGAGTTCC CTCTGGCCTT 
TGTGAAGGAC AGTATGCTGA AGGACATTCA GAATTTACCC CATTGGGGGT GTTTTACAAC 
AACAGGGTAC ACTCCAACTT CAAGGCCGGA TTATTCATTG GCAAAGGAGT CAAAACCACA 
GTAGCAAATG CTGAGAATCC AAGGGAATAT CTGACAGTAG ATAATGCCCG GTTTCGTCCC 
CATCAAGATT CAGATCCCAC CAAGCCTCGA ATACCAGCTG TTATTGATGG CCTAATAGCT 
TTCAAAAACA ATGACCATGG TGCATGGGCT AGAGGAGGGG ACATTATTTT CCGTAACTCA 
GGGTTTTCTG ATAATGGCAT TGGACTAACT TTAGCCAGTG ATGGGACATT TCCTACTGAT 
GAAGGCTCTA GCTTGGAGAT CACAGACTCC ATATTTGTGG GTGAGAGCGA GAATGTGGGA 
TCACAAGGTG GACAGAACAT CTACTGGGGA CGTGGGGGTT TGGGGGAGCA GCGATCATTT 
CCAAGAAACA AGACATTCCC AGTTCGTGGT TTCCAAATAT ACGATGGGCC CGTCAGGCTC 
CTGAAGTGCA CTTTCAAGAA ATTTCTCCCA ACTCTTGACA GACCTGTGAG CGCTATTGGG 
TTCTCCTTGA AAAATTCTTG GCAGATAACT CCACAGAACA ATGTGTCCCT GGTCAGGATG 
GAATGGAATG TTGGGCTGAA GGTTTTCTTT GGTCATTCAG GACAATGGTT TGGCTCAAAT 
AACTTTGATG GTGATAAAGT GTCAATCTTC CATGATTTGG ATGGCTCAGT GACTGGATAT 
CCTGATACCT TTATAGGAAG GGCAGATAAT TACCTGATCC GTCACCCTGG ATGCCTCACT 
GTGCCACGGT GGAACGGAGT GATTTGTTCA GGCAGGTATG CCCAGCTTTA CATCCAGGCC 
AGACGCCCTG AGAATCTGAA CATTTTGGTC TCTAGAGCAA CTTATCCCTC TGTTCCTTTA 
AAACTTTCTG GCGTTAACCG GGGTGCACCA TATCAACAGT ACCAACCAGT GGTCATGCTG 
CATCAATCCT ATATTATCAG ATGGGAAGGT CAGTCTCCTG GAGAGGTCAT CCTATACCCC 
ATCAACTTTA ACAGGGGAGA CTGGATTCAG ATTGCCTTGT GTTATCCCAG AAGTGCACAG 
TTCCAGGTCA TGTCAGATGT TTATGATCGT CACAGTGGAA GGATACACAG CGTAAGACAC 
TATTCCTGGG CTCATACCAT CAGCCAGGTT GTTAATCAGT CAGAGACAAG ACTTTTTCAT 
TTCAGTCAGG AATCTGGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100491930 ( XM_002932255.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932301.3 Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa35210
Clone ID Related Accession (Same CDS sequence)	XM_002932255.4
Accession Version	XM_002932255.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane protein 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932255.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTGAGAGAGG TCTAAACATG 
CTCCCAACAG CCAATATGGG ATGCTGTGCA CATGGACTCT TGTCTCCTTC CTCTGTAGAT 
GAAGACTGTC TGCAGATCCA CTTACACAGG CAATGGCACG GGGACACTGC TGACACTCAT 
AATTGTGACT CATGCCCAGC ACCTACTTAT CAACCAGGAG AAAAGTGGAG CAGACACCAA 
GACATTCTCA CTGTCACCTA TCTCTCCGGG AGAGGAAACA TACCTATGGG AAAACTTGCA 
GCCTTTCTGC CTTCATCACC CAGCCTTTCA CAAGTGTTCC TATTCCTTCT GATGCTTCTG 
TGCTGGCAGA GATGTGCAGC TGGAAATCTA AACTTCTTTC CAGAAGACAG CATCAATGCC 
AACATGTCTG TAGGAAACCT GGGTCCTGAT GAGTTGAGTG TGGTTAGAAA TGAAGCTCCA 
TCACTTTCCT ATGTCCATGG GGGAAATGCA TTGACTGAAG GAGACCCAGT GTTTGCACCA 
AGATACAACA TAACTCTGCA CAAAAAGGGC AAGCTATGGT CATGTGACAG TCTTCTTAAG 
CTCACTAGAC ATATTCTATT GACTATGTTG GGGCCTAGTT TGGCTTTTGA AGTCTTCCCA 
TATCATCACT GTAACCAGGA CTTAAAGCCA AGGGACACAC TTATTAGTCC ACTGAGGCTG 
TTACACGCGC ACGTGAAACC ACTTCTACTT CACAGAGTTT TAATATTCCT GGGGGAGCTC 
GGATTTTTGG TTGAATCCCA ACAAGCTAAA GCCCTGTCGG TACTGAAGAA GCAGATCTTC 
AAAACAGGAA AACCAATGAA GGAGGCTGGA GTGAAACCAA AGGACAATTG CCCAGATAAG 
AAGCCACATC TGATCCCTTG GAACCCAGGA CATGATGGAA GCAGATCAGC TGTTATCGGT 
TATGGTGTTG CCCTACGATT GGAGTCATCA GCCACTTTCC ACTCACTGCT TATTCAGAAT 
GGAGGATCTT TAGTGTTTGC AGATGACCTA GTGCCAATTA CTTTGAGAGC ACAGTATATT 
TTAATCAGAA ATGGCGGATC TTTTCACATT GGCTCAGAAA AGTGTCCTTA TAGGTCCAAT 
GCTACCATCA CGCTTTATGG GAAGTCTACA GAAGGTGACA ATGTAGAAGA CTTTGGAAAG 
AAGTTCTTAG GAGTAGATAC AGGAGGGATT TTGGAATTAC ATGGAAGGAA ACCATTATCA 
TGGACTTTGT TAGATGCAAC CTTACATCCA GGAGGGCTCC CCCATGGACC ATATAAGTGG 
GAGAAGCAAT GGGGCAGCAG GGGCATTAAC ATACGAATCA TTGACCCTGA AACTGGTCAA 
GTAATAGCTG CTGATCGCTT TGACACCCAC ATGTTTCTGG ATGAAGGGAA AAGGCTGGAG 
GAGTTCCTTG CTCAACAGAG TCCAGGCAAG ATAGTGGCAG CAGCAGTGGG TGACTCAGCA 
GCCAAGAGCT TAACCCCAGA CACAAGGAAA TTTCTAAGAG ATGCTTTGAG CAGCAAATAT 
ATCTTTCACT TGGGCTACAG GCAGCCCTGG GCTTTAGTTG GGGTGCTTGG AGAAGACCTG 
TTGATGGTAG CAGAGGACAG AAAGCAATAT GAGAGCAATG GGACCACTGG TCTTGCAACT 
GCCACAAGGG AATTCCAGAC ATATTCGGGG CTGCACTTCA CTGTAACTGC TTACAGTGGC 
TGGATTAAAG GAGTTCCGCG AAATGGATTC AGAGTGGCAG TGTCGAGGGG CATCATCCTC 
ACATTACTGG ATGATGTTAC AAGTTGGTTG CCTGGTAACA GAATAGTCGT TGCTAGTACC 
GATTACTCAA TGCATCAGAC TGAAGAATTC ACCCTGCTTC CCTGCCCTGA ATGCAGAAGT 
AATCAAGTCA AAATTAGCGG TAGCCCACTT TATTTGCATA TTGGTGAAGT TATTGATGGA 
ATTGACATGA GAGCAGAGGT TGGGGTTCTC ACAAGAAATG TTCTTATCCA TGGTGAAATG 
GAAGATTTAT GTTATGGACA AAACCAGTGT CAGTTTTTCA ACTATGATAC CTTTGGAGGA 
CAAGTCAAGA TTCAAGGAAG ATTTGCATCA GTTCATCTGT CAGGAGTAGA ACTAAAAAAC 
ATGGGGCAGC AAATCTTGGG CAGTTACCCA GTTCATTTCC ACATGACAGG AACTGTAGAT 
GAGGAAGGGG GCTATAACCC TCCAACGTAT GTGGACAATC TTTCCATCCA CCACTGTTTC 
TCGAGATGTG TGGTGATTCA TGGGACCCAT GGACTTCTGG TGAAAGACAC GATTGGCTAT 
GACACACTGG GGCACTGTTT TTTTCTTGAG GATGGTGTTG AACAAAGAAA TACATTTTAT 
CACAATCTAG GATTGCTGAC AAAGGCTGGG ACCATCTTGC CCACAGACCG AAGTGAAGCC 
ATGTGTCTTG CTATCCGAGA TTCTGTCTAT GGAGATTATG TTCCTGTGCC TAGCATTGAC 
TGCATGGCTG TCAGCACATT TTGGATTGCA AACCCTAATA ACAATTTAAT AGGAAATGCT 
GCAGGAGGGG CACAGGATGT TGGCATATGG TTCATATTCC ACCGAGTTCC CTCTGGCCTT 
TGTGAAGGAC AGTATGCTGA AGGACATTCA GAATTTACCC CATTGGGGGT GTTTTACAAC 
AACAGGGTAC ACTCCAACTT CAAGGCCGGA TTATTCATTG GCAAAGGAGT CAAAACCACA 
GTAGCAAATG CTGAGAATCC AAGGGAATAT CTGACAGTAG ATAATGCCCG GTTTCGTCCC 
CATCAAGATT CAGATCCCAC CAAGCCTCGA ATACCAGCTG TTATTGATGG CCTAATAGCT 
TTCAAAAACA ATGACCATGG TGCATGGGCT AGAGGAGGGG ACATTATTTT CCGTAACTCA 
GGGTTTTCTG ATAATGGCAT TGGACTAACT TTAGCCAGTG ATGGGACATT TCCTACTGAT 
GAAGGCTCTA GCTTGGAGAT CACAGACTCC ATATTTGTGG GTGAGAGCGA GAATGTGGGA 
TCACAAGGTG GACAGAACAT CTACTGGGGA CGTGGGGGTT TGGGGGAGCA GCGATCATTT 
CCAAGAAACA AGACATTCCC AGTTCGTGGT TTCCAAATAT ACGATGGGCC CGTCAGGCTC 
CTGAAGTGCA CTTTCAAGAA ATTTCTCCCA ACTCTTGACA GACCTGTGAG CGCTATTGGG 
TTCTCCTTGA AAAATTCTTG GCAGATAACT CCACAGAACA ATGTGTCCCT GGTCAGGATG 
GAATGGAATG TTGGGCTGAA GGTTTTCTTT GGTCATTCAG GACAATGGTT TGGCTCAAAT 
AACTTTGATG GTGATAAAGT GTCAATCTTC CATGATTTGG ATGGCTCAGT GACTGGATAT 
CCTGATACCT TTATAGGAAG GGCAGATAAT TACCTGATCC GTCACCCTGG ATGCCTCACT 
GTGCCACGGT GGAACGGAGT GATTTGTTCA GGCAGGTATG CCCAGCTTTA CATCCAGGCC 
AGACGCCCTG AGAATCTGAA CATTTTGGTC TCTAGAGCAA CTTATCCCTC TGTTCCTTTA 
AAACTTTCTG GCGTTAACCG GGGTGCACCA TATCAACAGT ACCAACCAGT GGTCATGCTG 
CATCAATCCT ATATTATCAG ATGGGAAGGT CAGTCTCCTG GAGAGGTCAT CCTATACCCC 
ATCAACTTTA ACAGGGGAGA CTGGATTCAG ATTGCCTTGT GTTATCCCAG AAGTGCACAG 
TTCCAGGTCA TGTCAGATGT TTATGATCGT CACAGTGGAA GGATACACAG CGTAAGACAC 
TATTCCTGGG CTCATACCAT CAGCCAGGTT GTTAATCAGT CAGAGACAAG ACTTTTTCAT 
TTCAGTCAGG AATCTGGGTA CTTGTTCATT CACCTACAGG CACACTCAGT CCGCAGTGGG 
CACAGCTATT GTTCCCAGCA TGGCTGTGAG CGAATTAAGA TAGTGTCTCA ATTTGAACTA 
GAGGATTCCA GTGAATGTAT TCAGAACCCA TTTCCTAAAA CAGATAGTTA TACCCATACC 
CGCCGACTCT TTGCATCTCA CCGACTTGCC CTGCCATGCA GTGAATGTGG TGCTCCACAG 
GTAGCAATAA CAAGCAACCC ATCTATGAAA TATACAAGAA TACAGATCCA ATCCCTCCGT 
ATGGCAGATA ATGTAAATGG ACTGAAAGCT GCATTTATCA AGGTTGATGA AGACATTTTT 
TTGATCAAGA GAAAGGGGCT TTTCTTCCTG GTGTTAGATG CCTGCTCTGG AGCTGTTGTA 
AGCAAGAGGC AATTTGAAAT GATCAGTGAT TTGAAGATAA CAAAAGCAAT TGCCAGTTAC 
ATTCAGACCT CAATGAAACT AAGATCAGTC GTTCTTATTT GCTCCAGGGA CATTGTGGAG 
ACTCCGCCTA GTTCTGAAAT GTCTGTTTTT ACTAAAATTG GCTCAGCCAA ACCCATTGTA 
CTTCCAAAGA AAGGTAGTTT TGCTATGGTA GGATTTAAAG GGCTTTCCAC TCCATCCTGG 
ATTAAAATCA TTAATAACAG TGCAGATAGA AAAGCTGCAT CGATTCACCT TTATCTTCCA 
CTGATGTTGA CTGAATATAG ATGCCCGGCC CCAAAACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932301.3
CDS	318..4979
Translation	MCLDRRQRRSKVTGERGLNMLPTANMGCCAHGLLSPSSVDEDCLQIHLHRQWHGDTADTHNCDSCPAPTYQPGEKWSRHQDILTVTYLSGRGNIPMGKLAAFLPSSPSLSQVFLFLLMLLCWQRCAAGNLNFFPEDSINANMSVGNLGPDELSVVRNEAPSLSYVHGGNALTEGDPVFAPRYNITLHKKGKLWSCDSLLKLTRHILLTMLGPSLAFEVFPYHHCNQDLKPRDTLISPLRLLHAHVKPLLLHRVLIFLGELGFLVESQQAKALSVLKKQIFKTGKPMKEAGVKPKDNCPDKKPHLIPWNPGHDGSRSAVIGYGVALRLESSATFHSLLIQNGGSLVFADDLVPITLRAQYILIRNGGSFHIGSEKCPYRSNATITLYGKSTEGDNVEDFGKKFLGVDTGGILELHGRKPLSWTLLDATLHPGGLPHGPYKWEKQWGSRGINIRIIDPETGQVIAADRFDTHMFLDEGKRLEEFLAQQSPGKIVAAAVGDSAAKSLTPDTRKFLRDALSSKYIFHLGYRQPWALVGVLGEDLLMVAEDRKQYESNGTTGLATATREFQTYSGLHFTVTAYSGWIKGVPRNGFRVAVSRGIILTLLDDVTSWLPGNRIVVASTDYSMHQTEEFTLLPCPECRSNQVKISGSPLYLHIGEVIDGIDMRAEVGVLTRNVLIHGEMEDLCYGQNQCQFFNYDTFGGQVKIQGRFASVHLSGVELKNMGQQILGSYPVHFHMTGTVDEEGGYNPPTYVDNLSIHHCFSRCVVIHGTHGLLVKDTIGYDTLGHCFFLEDGVEQRNTFYHNLGLLTKAGTILPTDRSEAMCLAIRDSVYGDYVPVPSIDCMAVSTFWIANPNNNLIGNAAGGAQDVGIWFIFHRVPSGLCEGQYAEGHSEFTPLGVFYNNRVHSNFKAGLFIGKGVKTTVANAENPREYLTVDNARFRPHQDSDPTKPRIPAVIDGLIAFKNNDHGAWARGGDIIFRNSGFSDNGIGLTLASDGTFPTDEGSSLEITDSIFVGESENVGSQGGQNIYWGRGGLGEQRSFPRNKTFPVRGFQIYDGPVRLLKCTFKKFLPTLDRPVSAIGFSLKNSWQITPQNNVSLVRMEWNVGLKVFFGHSGQWFGSNNFDGDKVSIFHDLDGSVTGYPDTFIGRADNYLIRHPGCLTVPRWNGVICSGRYAQLYIQARRPENLNILVSRATYPSVPLKLSGVNRGAPYQQYQPVVMLHQSYIIRWEGQSPGEVILYPINFNRGDWIQIALCYPRSAQFQVMSDVYDRHSGRIHSVRHYSWAHTISQVVNQSETRLFHFSQESGYLFIHLQAHSVRSGHSYCSQHGCERIKIVSQFELEDSSECIQNPFPKTDSYTHTRRLFASHRLALPCSECGAPQVAITSNPSMKYTRIQIQSLRMADNVNGLKAAFIKVDEDIFLIKRKGLFFLVLDACSGAVVSKRQFEMISDLKITKAIASYIQTSMKLRSVVLICSRDIVETPPSSEMSVFTKIGSAKPIVLPKKGSFAMVGFKGLSTPSWIKIINNSADRKAASIHLYLPLMLTEYRCPAPKL

Target ORF information:

RefSeq Version	XM_002932255.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932255.4

ORF Insert Sequence:

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTGAGAGAGG TCTAAACATG 
CTCCCAACAG CCAATATGGG ATGCTGTGCA CATGGACTCT TGTCTCCTTC CTCTGTAGAT 
GAAGACTGTC TGCAGATCCA CTTACACAGG CAATGGCACG GGGACACTGC TGACACTCAT 
AATTGTGACT CATGCCCAGC ACCTACTTAT CAACCAGGAG AAAAGTGGAG CAGACACCAA 
GACATTCTCA CTGTCACCTA TCTCTCCGGG AGAGGAAACA TACCTATGGG AAAACTTGCA 
GCCTTTCTGC CTTCATCACC CAGCCTTTCA CAAGTGTTCC TATTCCTTCT GATGCTTCTG 
TGCTGGCAGA GATGTGCAGC TGGAAATCTA AACTTCTTTC CAGAAGACAG CATCAATGCC 
AACATGTCTG TAGGAAACCT GGGTCCTGAT GAGTTGAGTG TGGTTAGAAA TGAAGCTCCA 
TCACTTTCCT ATGTCCATGG GGGAAATGCA TTGACTGAAG GAGACCCAGT GTTTGCACCA 
AGATACAACA TAACTCTGCA CAAAAAGGGC AAGCTATGGT CATGTGACAG TCTTCTTAAG 
CTCACTAGAC ATATTCTATT GACTATGTTG GGGCCTAGTT TGGCTTTTGA AGTCTTCCCA 
TATCATCACT GTAACCAGGA CTTAAAGCCA AGGGACACAC TTATTAGTCC ACTGAGGCTG 
TTACACGCGC ACGTGAAACC ACTTCTACTT CACAGAGTTT TAATATTCCT GGGGGAGCTC 
GGATTTTTGG TTGAATCCCA ACAAGCTAAA GCCCTGTCGG TACTGAAGAA GCAGATCTTC 
AAAACAGGAA AACCAATGAA GGAGGCTGGA GTGAAACCAA AGGACAATTG CCCAGATAAG 
AAGCCACATC TGATCCCTTG GAACCCAGGA CATGATGGAA GCAGATCAGC TGTTATCGGT 
TATGGTGTTG CCCTACGATT GGAGTCATCA GCCACTTTCC ACTCACTGCT TATTCAGAAT 
GGAGGATCTT TAGTGTTTGC AGATGACCTA GTGCCAATTA CTTTGAGAGC ACAGTATATT 
TTAATCAGAA ATGGCGGATC TTTTCACATT GGCTCAGAAA AGTGTCCTTA TAGGTCCAAT 
GCTACCATCA CGCTTTATGG GAAGTCTACA GAAGGTGACA ATGTAGAAGA CTTTGGAAAG 
AAGTTCTTAG GAGTAGATAC AGGAGGGATT TTGGAATTAC ATGGAAGGAA ACCATTATCA 
TGGACTTTGT TAGATGCAAC CTTACATCCA GGAGGGCTCC CCCATGGACC ATATAAGTGG 
GAGAAGCAAT GGGGCAGCAG GGGCATTAAC ATACGAATCA TTGACCCTGA AACTGGTCAA 
GTAATAGCTG CTGATCGCTT TGACACCCAC ATGTTTCTGG ATGAAGGGAA AAGGCTGGAG 
GAGTTCCTTG CTCAACAGAG TCCAGGCAAG ATAGTGGCAG CAGCAGTGGG TGACTCAGCA 
GCCAAGAGCT TAACCCCAGA CACAAGGAAA TTTCTAAGAG ATGCTTTGAG CAGCAAATAT 
ATCTTTCACT TGGGCTACAG GCAGCCCTGG GCTTTAGTTG GGGTGCTTGG AGAAGACCTG 
TTGATGGTAG CAGAGGACAG AAAGCAATAT GAGAGCAATG GGACCACTGG TCTTGCAACT 
GCCACAAGGG AATTCCAGAC ATATTCGGGG CTGCACTTCA CTGTAACTGC TTACAGTGGC 
TGGATTAAAG GAGTTCCGCG AAATGGATTC AGAGTGGCAG TGTCGAGGGG CATCATCCTC 
ACATTACTGG ATGATGTTAC AAGTTGGTTG CCTGGTAACA GAATAGTCGT TGCTAGTACC 
GATTACTCAA TGCATCAGAC TGAAGAATTC ACCCTGCTTC CCTGCCCTGA ATGCAGAAGT 
AATCAAGTCA AAATTAGCGG TAGCCCACTT TATTTGCATA TTGGTGAAGT TATTGATGGA 
ATTGACATGA GAGCAGAGGT TGGGGTTCTC ACAAGAAATG TTCTTATCCA TGGTGAAATG 
GAAGATTTAT GTTATGGACA AAACCAGTGT CAGTTTTTCA ACTATGATAC CTTTGGAGGA 
CAAGTCAAGA TTCAAGGAAG ATTTGCATCA GTTCATCTGT CAGGAGTAGA ACTAAAAAAC 
ATGGGGCAGC AAATCTTGGG CAGTTACCCA GTTCATTTCC ACATGACAGG AACTGTAGAT 
GAGGAAGGGG GCTATAACCC TCCAACGTAT GTGGACAATC TTTCCATCCA CCACTGTTTC 
TCGAGATGTG TGGTGATTCA TGGGACCCAT GGACTTCTGG TGAAAGACAC GATTGGCTAT 
GACACACTGG GGCACTGTTT TTTTCTTGAG GATGGTGTTG AACAAAGAAA TACATTTTAT 
CACAATCTAG GATTGCTGAC AAAGGCTGGG ACCATCTTGC CCACAGACCG AAGTGAAGCC 
ATGTGTCTTG CTATCCGAGA TTCTGTCTAT GGAGATTATG TTCCTGTGCC TAGCATTGAC 
TGCATGGCTG TCAGCACATT TTGGATTGCA AACCCTAATA ACAATTTAAT AGGAAATGCT 
GCAGGAGGGG CACAGGATGT TGGCATATGG TTCATATTCC ACCGAGTTCC CTCTGGCCTT 
TGTGAAGGAC AGTATGCTGA AGGACATTCA GAATTTACCC CATTGGGGGT GTTTTACAAC 
AACAGGGTAC ACTCCAACTT CAAGGCCGGA TTATTCATTG GCAAAGGAGT CAAAACCACA 
GTAGCAAATG CTGAGAATCC AAGGGAATAT CTGACAGTAG ATAATGCCCG GTTTCGTCCC 
CATCAAGATT CAGATCCCAC CAAGCCTCGA ATACCAGCTG TTATTGATGG CCTAATAGCT 
TTCAAAAACA ATGACCATGG TGCATGGGCT AGAGGAGGGG ACATTATTTT CCGTAACTCA 
GGGTTTTCTG ATAATGGCAT TGGACTAACT TTAGCCAGTG ATGGGACATT TCCTACTGAT 
GAAGGCTCTA GCTTGGAGAT CACAGACTCC ATATTTGTGG GTGAGAGCGA GAATGTGGGA 
TCACAAGGTG GACAGAACAT CTACTGGGGA CGTGGGGGTT TGGGGGAGCA GCGATCATTT 
CCAAGAAACA AGACATTCCC AGTTCGTGGT TTCCAAATAT ACGATGGGCC CGTCAGGCTC 
CTGAAGTGCA CTTTCAAGAA ATTTCTCCCA ACTCTTGACA GACCTGTGAG CGCTATTGGG 
TTCTCCTTGA AAAATTCTTG GCAGATAACT CCACAGAACA ATGTGTCCCT GGTCAGGATG 
GAATGGAATG TTGGGCTGAA GGTTTTCTTT GGTCATTCAG GACAATGGTT TGGCTCAAAT 
AACTTTGATG GTGATAAAGT GTCAATCTTC CATGATTTGG ATGGCTCAGT GACTGGATAT 
CCTGATACCT TTATAGGAAG GGCAGATAAT TACCTGATCC GTCACCCTGG ATGCCTCACT 
GTGCCACGGT GGAACGGAGT GATTTGTTCA GGCAGGTATG CCCAGCTTTA CATCCAGGCC 
AGACGCCCTG AGAATCTGAA CATTTTGGTC TCTAGAGCAA CTTATCCCTC TGTTCCTTTA 
AAACTTTCTG GCGTTAACCG GGGTGCACCA TATCAACAGT ACCAACCAGT GGTCATGCTG 
CATCAATCCT ATATTATCAG ATGGGAAGGT CAGTCTCCTG GAGAGGTCAT CCTATACCCC 
ATCAACTTTA ACAGGGGAGA CTGGATTCAG ATTGCCTTGT GTTATCCCAG AAGTGCACAG 
TTCCAGGTCA TGTCAGATGT TTATGATCGT CACAGTGGAA GGATACACAG CGTAAGACAC 
TATTCCTGGG CTCATACCAT CAGCCAGGTT GTTAATCAGT CAGAGACAAG ACTTTTTCAT 
TTCAGTCAGG AATCTGGGTA CTTGTTCATT CACCTACAGG CACACTCAGT CCGCAGTGGG 
CACAGCTATT GTTCCCAGCA TGGCTGTGAG CGAATTAAGA TAGTGTCTCA ATTTGAACTA 
GAGGATTCCA GTGAATGTAT TCAGAACCCA TTTCCTAAAA CAGATAGTTA TACCCATACC 
CGCCGACTCT TTGCATCTCA CCGACTTGCC CTGCCATGCA GTGAATGTGG TGCTCCACAG 
GTAGCAATAA CAAGCAACCC ATCTATGAAA TATACAAGAA TACAGATCCA ATCCCTCCGT 
ATGGCAGATA ATGTAAATGG ACTGAAAGCT GCATTTATCA AGGTTGATGA AGACATTTTT 
TTGATCAAGA GAAAGGGGCT TTTCTTCCTG GTGTTAGATG CCTGCTCTGG AGCTGTTGTA 
AGCAAGAGGC AATTTGAAAT GATCAGTGAT TTGAAGATAA CAAAAGCAAT TGCCAGTTAC 
ATTCAGACCT CAATGAAACT AAGATCAGTC GTTCTTATTT GCTCCAGGGA CATTGTGGAG 
ACTCCGCCTA GTTCTGAAAT GTCTGTTTTT ACTAAAATTG GCTCAGCCAA ACCCATTGTA 
CTTCCAAAGA AAGGTAGTTT TGCTATGGTA GGATTTAAAG GGCTTTCCAC TCCATCCTGG 
ATTAAAATCA TTAATAACAG TGCAGATAGA AAAGCTGCAT CGATTCACCT TTATCTTCCA 
CTGATGTTGA CTGAATATAG ATGCCCGGCC CCAAAACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100491930 ( XM_012959543.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814997.1 Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X2, mRNA.

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CloneID	OXa35211
Clone ID Related Accession (Same CDS sequence)	XM_012959543.2
Accession Version	XM_012959543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane protein 2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTCTAAACAT GCTCCCAACA 
GCCAATATGG GATGCTGTGC ACATGGACTC TTGTCTCCTT CCTCTGTAGA TGAAGACTGT 
CTGCAGATCC ACTTACACAG GCAATGGCAC GGGGACACTG CTGACACTCA TAATTGTGAC 
TCATGCCCAG CACCTACTTA TCAACCAGGA GAAAAGTGGA GCAGACACCA AGACATTCTC 
ACTGTCACCT ATCTCTCCGG GAGAGGAAAC ATACCTATGG GAAAACTTGC AGCCTTTCTG 
CCTTCATCAC CCAGCCTTTC ACAAGTGTTC CTATTCCTTC TGATGCTTCT GTGCTGGCAG 
AGATGTGCAG CTGGAAATCT AAACTTCTTT CCAGAAGACA GCATCAATGC CAACATGTCT 
GTAGGAAACC TGGGTCCTGA TGAGTTGAGT GTGGTTAGAA ATGAAGCTCC ATCACTTTCC 
TATGTCCATG GGGGAAATGC ATTGACTGAA GGAGACCCAG TGTTTGCACC AAGATACAAC 
ATAACTCTGC ACAAAAAGGG CAAGCTATGG TCATGTGACA GTCTTCTTAA GCTCACTAGA 
CATATTCTAT TGACTATGTT GGGGCCTAGT TTGGCTTTTG AAGTCTTCCC ATATCATCAC 
TGTAACCAGG ACTTAAAGCC AAGGGACACA CTTATTAGTC CACTGAGGCT GTTACACGCG 
CACGTGAAAC CACTTCTACT TCACAGAGTT TTAATATTCC TGGGGGAGCT CGGATTTTTG 
GTTGAATCCC AACAAGCTAA AGCCCTGTCG GTACTGAAGA AGCAGATCTT CAAAACAGGA 
AAACCAATGA AGGAGGCTGG AGTGAAACCA AAGGACAATT GCCCAGATAA GAAGCCACAT 
CTGATCCCTT GGAACCCAGG ACATGATGGA AGCAGATCAG CTGTTATCGG TTATGGTGTT 
GCCCTACGAT TGGAGTCATC AGCCACTTTC CACTCACTGC TTATTCAGAA TGGAGGATCT 
TTAGTGTTTG CAGATGACCT AGTGCCAATT ACTTTGAGAG CACAGTATAT TTTAATCAGA 
AATGGCGGAT CTTTTCACAT TGGCTCAGAA AAGTGTCCTT ATAGGTCCAA TGCTACCATC 
ACGCTTTATG GGAAGTCTAC AGAAGGTGAC AATGTAGAAG ACTTTGGAAA GAAGTTCTTA 
GGAGTAGATA CAGGAGGGAT TTTGGAATTA CATGGAAGGA AACCATTATC ATGGACTTTG 
TTAGATGCAA CCTTACATCC AGGAGGGCTC CCCCATGGAC CATATAAGTG GGAGAAGCAA 
TGGGGCAGCA GGGGCATTAA CATACGAATC ATTGACCCTG AAACTGGTCA AGTAATAGCT 
GCTGATCGCT TTGACACCCA CATGTTTCTG GATGAAGGGA AAAGGCTGGA GGAGTTCCTT 
GCTCAACAGA GTCCAGGCAA GATAGTGGCA GCAGCAGTGG GTGACTCAGC AGCCAAGAGC 
TTAACCCCAG ACACAAGGAA ATTTCTAAGA GATGCTTTGA GCAGCAAATA TATCTTTCAC 
TTGGGCTACA GGCAGCCCTG GGCTTTAGTT GGGGTGCTTG GAGAAGACCT GTTGATGGTA 
GCAGAGGACA GAAAGCAATA TGAGAGCAAT GGGACCACTG GTCTTGCAAC TGCCACAAGG 
GAATTCCAGA CATATTCGGG GCTGCACTTC ACTGTAACTG CTTACAGTGG CTGGATTAAA 
GGAGTTCCGC GAAATGGATT CAGAGTGGCA GTGTCGAGGG GCATCATCCT CACATTACTG 
GATGATGTTA CAAGTTGGTT GCCTGGTAAC AGAATAGTCG TTGCTAGTAC CGATTACTCA 
ATGCATCAGA CTGAAGAATT CACCCTGCTT CCCTGCCCTG AATGCAGAAG TAATCAAGTC 
AAAATTAGCG GTAGCCCACT TTATTTGCAT ATTGGTGAAG TTATTGATGG AATTGACATG 
AGAGCAGAGG TTGGGGTTCT CACAAGAAAT GTTCTTATCC ATGGTGAAAT GGAAGATTTA 
TGTTATGGAC AAAACCAGTG TCAGTTTTTC AACTATGATA CCTTTGGAGG ACAAGTCAAG 
ATTCAAGGAA GATTTGCATC AGTTCATCTG TCAGGAGTAG AACTAAAAAA CATGGGGCAG 
CAAATCTTGG GCAGTTACCC AGTTCATTTC CACATGACAG GAACTGTAGA TGAGGAAGGG 
GGCTATAACC CTCCAACGTA TGTGGACAAT CTTTCCATCC ACCACTGTTT CTCGAGATGT 
GTGGTGATTC ATGGGACCCA TGGACTTCTG GTGAAAGACA CGATTGGCTA TGACACACTG 
GGGCACTGTT TTTTTCTTGA GGATGGTGTT GAACAAAGAA ATACATTTTA TCACAATCTA 
GGATTGCTGA CAAAGGCTGG GACCATCTTG CCCACAGACC GAAGTGAAGC CATGTGTCTT 
GCTATCCGAG ATTCTGTCTA TGGAGATTAT GTTCCTGTGC CTAGCATTGA CTGCATGGCT 
GTCAGCACAT TTTGGATTGC AAACCCTAAT AACAATTTAA TAGGAAATGC TGCAGGAGGG 
GCACAGGATG TTGGCATATG GTTCATATTC CACCGAGTTC CCTCTGGCCT TTGTGAAGGA 
CAGTATGCTG AAGGACATTC AGAATTTACC CCATTGGGGG TGTTTTACAA CAACAGGGTA 
CACTCCAACT TCAAGGCCGG ATTATTCATT GGCAAAGGAG TCAAAACCAC AGTAGCAAAT 
GCTGAGAATC CAAGGGAATA TCTGACAGTA GATAATGCCC GGTTTCGTCC CCATCAAGAT 
TCAGATCCCA CCAAGCCTCG AATACCAGCT GTTATTGATG GCCTAATAGC TTTCAAAAAC 
AATGACCATG GTGCATGGGC TAGAGGAGGG GACATTATTT TCCGTAACTC AGGGTTTTCT 
GATAATGGCA TTGGACTAAC TTTAGCCAGT GATGGGACAT TTCCTACTGA TGAAGGCTCT 
AGCTTGGAGA TCACAGACTC CATATTTGTG GGTGAGAGCG AGAATGTGGG ATCACAAGGT 
GGACAGAACA TCTACTGGGG ACGTGGGGGT TTGGGGGAGC AGCGATCATT TCCAAGAAAC 
AAGACATTCC CAGTTCGTGG TTTCCAAATA TACGATGGGC CCGTCAGGCT CCTGAAGTGC 
ACTTTCAAGA AATTTCTCCC AACTCTTGAC AGACCTGTGA GCGCTATTGG GTTCTCCTTG 
AAAAATTCTT GGCAGATAAC TCCACAGAAC AATGTGTCCC TGGTCAGGAT GGAATGGAAT 
GTTGGGCTGA AGGTTTTCTT TGGTCATTCA GGACAATGGT TTGGCTCAAA TAACTTTGAT 
GGTGATAAAG TGTCAATCTT CCATGATTTG GATGGCTCAG TGACTGGATA TCCTGATACC 
TTTATAGGAA GGGCAGATAA TTACCTGATC CGTCACCCTG GATGCCTCAC TGTGCCACGG 
TGGAACGGAG TGATTTGTTC AGGCAGGTAT GCCCAGCTTT ACATCCAGGC CAGACGCCCT 
GAGAATCTGA ACATTTTGGT CTCTAGAGCA ACTTATCCCT CTGTTCCTTT AAAACTTTCT 
GGCGTTAACC GGGGTGCACC ATATCAACAG TACCAACCAG TGGTCATGCT GCATCAATCC 
TATATTATCA GATGGGAAGG TCAGTCTCCT GGAGAGGTCA TCCTATACCC CATCAACTTT 
AACAGGGGAG ACTGGATTCA GATTGCCTTG TGTTATCCCA GAAGTGCACA GTTCCAGGTC 
ATGTCAGATG TTTATGATCG TCACAGTGGA AGGATACACA GCGTAAGACA CTATTCCTGG 
GCTCATACCA TCAGCCAGGT TGTTAATCAG TCAGAGACAA GACTTTTTCA TTTCAGTCAG 
GAATCTGGGT ACTTGTTCAT TCACCTACAG GCACACTCAG TCCGCAGTGG GCACAGCTAT 
TGTTCCCAGC ATGGCTGTGA GCGAATTAAG ATAGTGTCTC AATTTGAACT AGAGGATTCC 
AGTGAATGTA TTCAGAACCC ATTTCCTAAA ACAGATAGTT ATACCCATAC CCGCCGACTC 
TTTGCATCTC ACCGACTTGC CCTGCCATGC AGTGAATGTG GTGCTCCACA GGTAGCAATA 
ACAAGCAACC CATCTATGAA ATATACAAGA ATACAGATCC AATCCCTCCG TATGGCAGAT 
AATGTAAATG GACTGAAAGC TGCATTTATC AAGGTTGATG AAGACATTTT TTTGATCAAG 
AGAAAGGGGC TTTTCTTCCT GGTGTTAGAT GCCTGCTCTG GAGCTGTTGT AAGCAAGAGG 
CAATTTGAAA TGATCAGTGA TTTGAAGATA ACAAAAGCAA TTGCCAGTTA CATTCAGACC 
TCAATGAAAC TAAGATCAGT CGTTCTTATT TGCTCCAGGG ACATTGTGGA GACTCCGCCT 
AGTTCTGAAA TGTCTGTTTT TACTAAAATT GGCTCAGCCA AACCCATTGT ACTTCCAAAG 
AAAGGTAGTT TTGCTATGGT AGGATTTAAA GGGCTTTCCA CTCCATCCTG GATTAAAATC 
ATTAATAACA GTGCAGATAG AAAAGCTGCA TCGATTCACC TTTATCTTCC ACTGATGTTG 
ACTGAATATA GATGCCCGGC CCCAAAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814997.1
CDS	318..4970
Translation	MCLDRRQRRSKVTGLNMLPTANMGCCAHGLLSPSSVDEDCLQIHLHRQWHGDTADTHNCDSCPAPTYQPGEKWSRHQDILTVTYLSGRGNIPMGKLAAFLPSSPSLSQVFLFLLMLLCWQRCAAGNLNFFPEDSINANMSVGNLGPDELSVVRNEAPSLSYVHGGNALTEGDPVFAPRYNITLHKKGKLWSCDSLLKLTRHILLTMLGPSLAFEVFPYHHCNQDLKPRDTLISPLRLLHAHVKPLLLHRVLIFLGELGFLVESQQAKALSVLKKQIFKTGKPMKEAGVKPKDNCPDKKPHLIPWNPGHDGSRSAVIGYGVALRLESSATFHSLLIQNGGSLVFADDLVPITLRAQYILIRNGGSFHIGSEKCPYRSNATITLYGKSTEGDNVEDFGKKFLGVDTGGILELHGRKPLSWTLLDATLHPGGLPHGPYKWEKQWGSRGINIRIIDPETGQVIAADRFDTHMFLDEGKRLEEFLAQQSPGKIVAAAVGDSAAKSLTPDTRKFLRDALSSKYIFHLGYRQPWALVGVLGEDLLMVAEDRKQYESNGTTGLATATREFQTYSGLHFTVTAYSGWIKGVPRNGFRVAVSRGIILTLLDDVTSWLPGNRIVVASTDYSMHQTEEFTLLPCPECRSNQVKISGSPLYLHIGEVIDGIDMRAEVGVLTRNVLIHGEMEDLCYGQNQCQFFNYDTFGGQVKIQGRFASVHLSGVELKNMGQQILGSYPVHFHMTGTVDEEGGYNPPTYVDNLSIHHCFSRCVVIHGTHGLLVKDTIGYDTLGHCFFLEDGVEQRNTFYHNLGLLTKAGTILPTDRSEAMCLAIRDSVYGDYVPVPSIDCMAVSTFWIANPNNNLIGNAAGGAQDVGIWFIFHRVPSGLCEGQYAEGHSEFTPLGVFYNNRVHSNFKAGLFIGKGVKTTVANAENPREYLTVDNARFRPHQDSDPTKPRIPAVIDGLIAFKNNDHGAWARGGDIIFRNSGFSDNGIGLTLASDGTFPTDEGSSLEITDSIFVGESENVGSQGGQNIYWGRGGLGEQRSFPRNKTFPVRGFQIYDGPVRLLKCTFKKFLPTLDRPVSAIGFSLKNSWQITPQNNVSLVRMEWNVGLKVFFGHSGQWFGSNNFDGDKVSIFHDLDGSVTGYPDTFIGRADNYLIRHPGCLTVPRWNGVICSGRYAQLYIQARRPENLNILVSRATYPSVPLKLSGVNRGAPYQQYQPVVMLHQSYIIRWEGQSPGEVILYPINFNRGDWIQIALCYPRSAQFQVMSDVYDRHSGRIHSVRHYSWAHTISQVVNQSETRLFHFSQESGYLFIHLQAHSVRSGHSYCSQHGCERIKIVSQFELEDSSECIQNPFPKTDSYTHTRRLFASHRLALPCSECGAPQVAITSNPSMKYTRIQIQSLRMADNVNGLKAAFIKVDEDIFLIKRKGLFFLVLDACSGAVVSKRQFEMISDLKITKAIASYIQTSMKLRSVVLICSRDIVETPPSSEMSVFTKIGSAKPIVLPKKGSFAMVGFKGLSTPSWIKIINNSADRKAASIHLYLPLMLTEYRCPAPKL

Target ORF information:

RefSeq Version	XM_012959543.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 2-like (LOC100491930), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959543.2

ORF Insert Sequence:

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTCTAAACAT GCTCCCAACA 
GCCAATATGG GATGCTGTGC ACATGGACTC TTGTCTCCTT CCTCTGTAGA TGAAGACTGT 
CTGCAGATCC ACTTACACAG GCAATGGCAC GGGGACACTG CTGACACTCA TAATTGTGAC 
TCATGCCCAG CACCTACTTA TCAACCAGGA GAAAAGTGGA GCAGACACCA AGACATTCTC 
ACTGTCACCT ATCTCTCCGG GAGAGGAAAC ATACCTATGG GAAAACTTGC AGCCTTTCTG 
CCTTCATCAC CCAGCCTTTC ACAAGTGTTC CTATTCCTTC TGATGCTTCT GTGCTGGCAG 
AGATGTGCAG CTGGAAATCT AAACTTCTTT CCAGAAGACA GCATCAATGC CAACATGTCT 
GTAGGAAACC TGGGTCCTGA TGAGTTGAGT GTGGTTAGAA ATGAAGCTCC ATCACTTTCC 
TATGTCCATG GGGGAAATGC ATTGACTGAA GGAGACCCAG TGTTTGCACC AAGATACAAC 
ATAACTCTGC ACAAAAAGGG CAAGCTATGG TCATGTGACA GTCTTCTTAA GCTCACTAGA 
CATATTCTAT TGACTATGTT GGGGCCTAGT TTGGCTTTTG AAGTCTTCCC ATATCATCAC 
TGTAACCAGG ACTTAAAGCC AAGGGACACA CTTATTAGTC CACTGAGGCT GTTACACGCG 
CACGTGAAAC CACTTCTACT TCACAGAGTT TTAATATTCC TGGGGGAGCT CGGATTTTTG 
GTTGAATCCC AACAAGCTAA AGCCCTGTCG GTACTGAAGA AGCAGATCTT CAAAACAGGA 
AAACCAATGA AGGAGGCTGG AGTGAAACCA AAGGACAATT GCCCAGATAA GAAGCCACAT 
CTGATCCCTT GGAACCCAGG ACATGATGGA AGCAGATCAG CTGTTATCGG TTATGGTGTT 
GCCCTACGAT TGGAGTCATC AGCCACTTTC CACTCACTGC TTATTCAGAA TGGAGGATCT 
TTAGTGTTTG CAGATGACCT AGTGCCAATT ACTTTGAGAG CACAGTATAT TTTAATCAGA 
AATGGCGGAT CTTTTCACAT TGGCTCAGAA AAGTGTCCTT ATAGGTCCAA TGCTACCATC 
ACGCTTTATG GGAAGTCTAC AGAAGGTGAC AATGTAGAAG ACTTTGGAAA GAAGTTCTTA 
GGAGTAGATA CAGGAGGGAT TTTGGAATTA CATGGAAGGA AACCATTATC ATGGACTTTG 
TTAGATGCAA CCTTACATCC AGGAGGGCTC CCCCATGGAC CATATAAGTG GGAGAAGCAA 
TGGGGCAGCA GGGGCATTAA CATACGAATC ATTGACCCTG AAACTGGTCA AGTAATAGCT 
GCTGATCGCT TTGACACCCA CATGTTTCTG GATGAAGGGA AAAGGCTGGA GGAGTTCCTT 
GCTCAACAGA GTCCAGGCAA GATAGTGGCA GCAGCAGTGG GTGACTCAGC AGCCAAGAGC 
TTAACCCCAG ACACAAGGAA ATTTCTAAGA GATGCTTTGA GCAGCAAATA TATCTTTCAC 
TTGGGCTACA GGCAGCCCTG GGCTTTAGTT GGGGTGCTTG GAGAAGACCT GTTGATGGTA 
GCAGAGGACA GAAAGCAATA TGAGAGCAAT GGGACCACTG GTCTTGCAAC TGCCACAAGG 
GAATTCCAGA CATATTCGGG GCTGCACTTC ACTGTAACTG CTTACAGTGG CTGGATTAAA 
GGAGTTCCGC GAAATGGATT CAGAGTGGCA GTGTCGAGGG GCATCATCCT CACATTACTG 
GATGATGTTA CAAGTTGGTT GCCTGGTAAC AGAATAGTCG TTGCTAGTAC CGATTACTCA 
ATGCATCAGA CTGAAGAATT CACCCTGCTT CCCTGCCCTG AATGCAGAAG TAATCAAGTC 
AAAATTAGCG GTAGCCCACT TTATTTGCAT ATTGGTGAAG TTATTGATGG AATTGACATG 
AGAGCAGAGG TTGGGGTTCT CACAAGAAAT GTTCTTATCC ATGGTGAAAT GGAAGATTTA 
TGTTATGGAC AAAACCAGTG TCAGTTTTTC AACTATGATA CCTTTGGAGG ACAAGTCAAG 
ATTCAAGGAA GATTTGCATC AGTTCATCTG TCAGGAGTAG AACTAAAAAA CATGGGGCAG 
CAAATCTTGG GCAGTTACCC AGTTCATTTC CACATGACAG GAACTGTAGA TGAGGAAGGG 
GGCTATAACC CTCCAACGTA TGTGGACAAT CTTTCCATCC ACCACTGTTT CTCGAGATGT 
GTGGTGATTC ATGGGACCCA TGGACTTCTG GTGAAAGACA CGATTGGCTA TGACACACTG 
GGGCACTGTT TTTTTCTTGA GGATGGTGTT GAACAAAGAA ATACATTTTA TCACAATCTA 
GGATTGCTGA CAAAGGCTGG GACCATCTTG CCCACAGACC GAAGTGAAGC CATGTGTCTT 
GCTATCCGAG ATTCTGTCTA TGGAGATTAT GTTCCTGTGC CTAGCATTGA CTGCATGGCT 
GTCAGCACAT TTTGGATTGC AAACCCTAAT AACAATTTAA TAGGAAATGC TGCAGGAGGG 
GCACAGGATG TTGGCATATG GTTCATATTC CACCGAGTTC CCTCTGGCCT TTGTGAAGGA 
CAGTATGCTG AAGGACATTC AGAATTTACC CCATTGGGGG TGTTTTACAA CAACAGGGTA 
CACTCCAACT TCAAGGCCGG ATTATTCATT GGCAAAGGAG TCAAAACCAC AGTAGCAAAT 
GCTGAGAATC CAAGGGAATA TCTGACAGTA GATAATGCCC GGTTTCGTCC CCATCAAGAT 
TCAGATCCCA CCAAGCCTCG AATACCAGCT GTTATTGATG GCCTAATAGC TTTCAAAAAC 
AATGACCATG GTGCATGGGC TAGAGGAGGG GACATTATTT TCCGTAACTC AGGGTTTTCT 
GATAATGGCA TTGGACTAAC TTTAGCCAGT GATGGGACAT TTCCTACTGA TGAAGGCTCT 
AGCTTGGAGA TCACAGACTC CATATTTGTG GGTGAGAGCG AGAATGTGGG ATCACAAGGT 
GGACAGAACA TCTACTGGGG ACGTGGGGGT TTGGGGGAGC AGCGATCATT TCCAAGAAAC 
AAGACATTCC CAGTTCGTGG TTTCCAAATA TACGATGGGC CCGTCAGGCT CCTGAAGTGC 
ACTTTCAAGA AATTTCTCCC AACTCTTGAC AGACCTGTGA GCGCTATTGG GTTCTCCTTG 
AAAAATTCTT GGCAGATAAC TCCACAGAAC AATGTGTCCC TGGTCAGGAT GGAATGGAAT 
GTTGGGCTGA AGGTTTTCTT TGGTCATTCA GGACAATGGT TTGGCTCAAA TAACTTTGAT 
GGTGATAAAG TGTCAATCTT CCATGATTTG GATGGCTCAG TGACTGGATA TCCTGATACC 
TTTATAGGAA GGGCAGATAA TTACCTGATC CGTCACCCTG GATGCCTCAC TGTGCCACGG 
TGGAACGGAG TGATTTGTTC AGGCAGGTAT GCCCAGCTTT ACATCCAGGC CAGACGCCCT 
GAGAATCTGA ACATTTTGGT CTCTAGAGCA ACTTATCCCT CTGTTCCTTT AAAACTTTCT 
GGCGTTAACC GGGGTGCACC ATATCAACAG TACCAACCAG TGGTCATGCT GCATCAATCC 
TATATTATCA GATGGGAAGG TCAGTCTCCT GGAGAGGTCA TCCTATACCC CATCAACTTT 
AACAGGGGAG ACTGGATTCA GATTGCCTTG TGTTATCCCA GAAGTGCACA GTTCCAGGTC 
ATGTCAGATG TTTATGATCG TCACAGTGGA AGGATACACA GCGTAAGACA CTATTCCTGG 
GCTCATACCA TCAGCCAGGT TGTTAATCAG TCAGAGACAA GACTTTTTCA TTTCAGTCAG 
GAATCTGGGT ACTTGTTCAT TCACCTACAG GCACACTCAG TCCGCAGTGG GCACAGCTAT 
TGTTCCCAGC ATGGCTGTGA GCGAATTAAG ATAGTGTCTC AATTTGAACT AGAGGATTCC 
AGTGAATGTA TTCAGAACCC ATTTCCTAAA ACAGATAGTT ATACCCATAC CCGCCGACTC 
TTTGCATCTC ACCGACTTGC CCTGCCATGC AGTGAATGTG GTGCTCCACA GGTAGCAATA 
ACAAGCAACC CATCTATGAA ATATACAAGA ATACAGATCC AATCCCTCCG TATGGCAGAT 
AATGTAAATG GACTGAAAGC TGCATTTATC AAGGTTGATG AAGACATTTT TTTGATCAAG 
AGAAAGGGGC TTTTCTTCCT GGTGTTAGAT GCCTGCTCTG GAGCTGTTGT AAGCAAGAGG 
CAATTTGAAA TGATCAGTGA TTTGAAGATA ACAAAAGCAA TTGCCAGTTA CATTCAGACC 
TCAATGAAAC TAAGATCAGT CGTTCTTATT TGCTCCAGGG ACATTGTGGA GACTCCGCCT 
AGTTCTGAAA TGTCTGTTTT TACTAAAATT GGCTCAGCCA AACCCATTGT ACTTCCAAAG 
AAAGGTAGTT TTGCTATGGT AGGATTTAAA GGGCTTTCCA CTCCATCCTG GATTAAAATC 
ATTAATAACA GTGCAGATAG AAAAGCTGCA TCGATTCACC TTTATCTTCC ACTGATGTTG 
ACTGAATATA GATGCCCGGC CCCAAAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100491930 ( XM_002932255.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932301.4 Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X1, mRNA.

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CloneID	OXa55177
Clone ID Related Accession (Same CDS sequence)	XM_002932255.5
Accession Version	XM_002932255.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cell surface hyaluronidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932255.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTGAGAGAGG TCTAAACATG 
CTCCCAACAG CCAATATGGG ATGCTGTGCA CATGGACTCT TGTCTCCTTC CTCTGTAGAT 
GAAGACTGTC TGCAGATCCA CTTACACAGG CAATGGCACG GGGACACTGC TGACACTCAT 
AATTGTGACT CATGCCCAGC ACCTACTTAT CAACCAGGAG AAAAGTGGAG CAGACACCAA 
GACATTCTCA CTGTCACCTA TCTCTCCGGG AGAGGAAACA TACCTATGGG AAAACTTGCA 
GCCTTTCTGC CTTCATCACC CAGCCTTTCA CAAGTGTTCC TATTCCTTCT GATGCTTCTG 
TGCTGGCAGA GATGTGCAGC TGGAAATCTA AACTTCTTTC CAGAAGACAG CATCAATGCC 
AACATGTCTG TAGGAAACCT GGGTCCTGAT GAGTTGAGTG TGGTTAGAAA TGAAGCTCCA 
TCACTTTCCT ATGTCCATGG GGGAAATGCA TTGACTGAAG GAGACCCAGT GTTTGCACCA 
AGATACAACA TAACTCTGCA CAAAAAGGGC AAGCTATGGT CATGTGACAG TCTTCTTAAG 
CTCACTAGAC ATATTCTATT GACTATGTTG GGGCCTAGTT TGGCTTTTGA AGTCTTCCCA 
TATCATCACT GTAACCAGGA CTTAAAGCCA AGGGACACAC TTATTAGTCC ACTGAGGCTG 
TTACACGCGC ACGTGAAACC ACTTCTACTT CACAGAGTTT TAATATTCCT GGGGGAGCTC 
GGATTTTTGG TTGAATCCCA ACAAGCTAAA GCCCTGTCGG TACTGAAGAA GCAGATCTTC 
AAAACAGGAA AACCAATGAA GGAGGCTGGA GTGAAACCAA AGGACAATTG CCCAGATAAG 
AAGCCACATC TGATCCCTTG GAACCCAGGA CATGATGGAA GCAGATCAGC TGTTATCGGT 
TATGGTGTTG CCCTACGATT GGAGTCATCA GCCACTTTCC ACTCACTGCT TATTCAGAAT 
GGAGGATCTT TAGTGTTTGC AGATGACCTA GTGCCAATTA CTTTGAGAGC ACAGTATATT 
TTAATCAGAA ATGGCGGATC TTTTCACATT GGCTCAGAAA AGTGTCCTTA TAGGTCCAAT 
GCTACCATCA CGCTTTATGG GAAGTCTACA GAAGGTGACA ATGTAGAAGA CTTTGGAAAG 
AAGTTCTTAG GAGTAGATAC AGGAGGGATT TTGGAATTAC ATGGAAGGAA ACCATTATCA 
TGGACTTTGT TAGATGCAAC CTTACATCCA GGAGGGCTCC CCCATGGACC ATATAAGTGG 
GAGAAGCAAT GGGGCAGCAG GGGCATTAAC ATACGAATCA TTGACCCTGA AACTGGTCAA 
GTAATAGCTG CTGATCGCTT TGACACCCAC ATGTTTCTGG ATGAAGGGAA AAGGCTGGAG 
GAGTTCCTTG CTCAACAGAG TCCAGGCAAG ATAGTGGCAG CAGCAGTGGG TGACTCAGCA 
GCCAAGAGCT TAACCCCAGA CACAAGGAAA TTTCTAAGAG ATGCTTTGAG CAGCAAATAT 
ATCTTTCACT TGGGCTACAG GCAGCCCTGG GCTTTAGTTG GGGTGCTTGG AGAAGACCTG 
TTGATGGTAG CAGAGGACAG AAAGCAATAT GAGAGCAATG GGACCACTGG TCTTGCAACT 
GCCACAAGGG AATTCCAGAC ATATTCGGGG CTGCACTTCA CTGTAACTGC TTACAGTGGC 
TGGATTAAAG GAGTTCCGCG AAATGGATTC AGAGTGGCAG TGTCGAGGGG CATCATCCTC 
ACATTACTGG ATGATGTTAC AAGTTGGTTG CCTGGTAACA GAATAGTCGT TGCTAGTACC 
GATTACTCAA TGCATCAGAC TGAAGAATTC ACCCTGCTTC CCTGCCCTGA ATGCAGAAGT 
AATCAAGTCA AAATTAGCGG TAGCCCACTT TATTTGCATA TTGGTGAAGT TATTGATGGA 
ATTGACATGA GAGCAGAGGT TGGGGTTCTC ACAAGAAATG TTCTTATCCA TGGTGAAATG 
GAAGATTTAT GTTATGGACA AAACCAGTGT CAGTTTTTCA ACTATGATAC CTTTGGAGGA 
CAAGTCAAGA TTCAAGGAAG ATTTGCATCA GTTCATCTGT CAGGAGTAGA ACTAAAAAAC 
ATGGGGCAGC AAATCTTGGG CAGTTACCCA GTTCATTTCC ACATGACAGG AACTGTAGAT 
GAGGAAGGGG GCTATAACCC TCCAACGTAT GTGGACAATC TTTCCATCCA CCACTGTTTC 
TCGAGATGTG TGGTGATTCA TGGGACCCAT GGACTTCTGG TGAAAGACAC GATTGGCTAT 
GACACACTGG GGCACTGTTT TTTTCTTGAG GATGGTGTTG AACAAAGAAA TACATTTTAT 
CACAATCTAG GATTGCTGAC AAAGGCTGGG ACCATCTTGC CCACAGACCG AAGTGAAGCC 
ATGTGTCTTG CTATCCGAGA TTCTGTCTAT GGAGATTATG TTCCTGTGCC TAGCATTGAC 
TGCATGGCTG TCAGCACATT TTGGATTGCA AACCCTAATA ACAATTTAAT AGGAAATGCT 
GCAGGAGGGG CACAGGATGT TGGCATATGG TTCATATTCC ACCGAGTTCC CTCTGGCCTT 
TGTGAAGGAC AGTATGCTGA AGGACATTCA GAATTTACCC CATTGGGGGT GTTTTACAAC 
AACAGGGTAC ACTCCAACTT CAAGGCCGGA TTATTCATTG GCAAAGGAGT CAAAACCACA 
GTAGCAAATG CTGAGAATCC AAGGGAATAT CTGACAGTAG ATAATGCCCG GTTTCGTCCC 
CATCAAGATT CAGATCCCAC CAAGCCTCGA ATACCAGCTG TTATTGATGG CCTAATAGCT 
TTCAAAAACA ATGACCATGG TGCATGGGCT AGAGGAGGGG ACATTATTTT CCGTAACTCA 
GGGTTTTCTG ATAATGGCAT TGGACTAACT TTAGCCAGTG ATGGGACATT TCCTACTGAT 
GAAGGCTCTA GCTTGGAGAT CACAGACTCC ATATTTGTGG GTGAGAGCGA GAATGTGGGA 
TCACAAGGTG GACAGAACAT CTACTGGGGA CGTGGGGGTT TGGGGGAGCA GCGATCATTT 
CCAAGAAACA AGACATTCCC AGTTCGTGGT TTCCAAATAT ACGATGGGCC CGTCAGGCTC 
CTGAAGTGCA CTTTCAAGAA ATTTCTCCCA ACTCTTGACA GACCTGTGAG CGCTATTGGG 
TTCTCCTTGA AAAATTCTTG GCAGATAACT CCACAGAACA ATGTGTCCCT GGTCAGGATG 
GAATGGAATG TTGGGCTGAA GGTTTTCTTT GGTCATTCAG GACAATGGTT TGGCTCAAAT 
AACTTTGATG GTGATAAAGT GTCAATCTTC CATGATTTGG ATGGCTCAGT GACTGGATAT 
CCTGATACCT TTATAGGAAG GGCAGATAAT TACCTGATCC GTCACCCTGG ATGCCTCACT 
GTGCCACGGT GGAACGGAGT GATTTGTTCA GGCAGGTATG CCCAGCTTTA CATCCAGGCC 
AGACGCCCTG AGAATCTGAA CATTTTGGTC TCTAGAGCAA CTTATCCCTC TGTTCCTTTA 
AAACTTTCTG GCGTTAACCG GGGTGCACCA TATCAACAGT ACCAACCAGT GGTCATGCTG 
CATCAATCCT ATATTATCAG ATGGGAAGGT CAGTCTCCTG GAGAGGTCAT CCTATACCCC 
ATCAACTTTA ACAGGGGAGA CTGGATTCAG ATTGCCTTGT GTTATCCCAG AAGTGCACAG 
TTCCAGGTCA TGTCAGATGT TTATGATCGT CACAGTGGAA GGATACACAG CGTAAGACAC 
TATTCCTGGG CTCATACCAT CAGCCAGGTT CTTAATCAGT CAGAGACAAG ACTTTTTCAT 
TTCAGTCAGG AATCTGGGTA CTTGTTCATA CACCTACAGG CACACTCAGT CCGCAGTGGG 
CACAGCTATT GTTCCCAGCA TGGCTGTGAG CGAATTAAGA TAGTGTCTCA ATTTGAACTA 
GAGGATTCCA GTGAATGTAT TCAGAACCCA TTTCCTAAAA CAGATAGTTA TACCCATACC 
CGCCGACTCT TTGCATCTCA CCGACTTGCC CTGCCATGCA GTGAATGTGG TGCTCCACAG 
GTAGCAATAA CAAGCAACCC ATCTATGAAA TATACAAGAA TACAGATCCA ATCCCTCCGT 
ATGGCAGATA ATGTAAATGG ACTGAAAGCT GCATTTATCA AGGTTGATGA AGACATTTTT 
TTGATCAAGA GAAAGGGGCT TTTCTTCCTG GTGTTAGATG CCTGCTCTGG AGCTGTTGTA 
AGCAAGAGGC AATTTGAAAT GATCAGTGAT TTGAAGATAA CAAAAGCAAT TGCCAGTTAC 
ATTCAGACCT CAATGAAACT AAGATCAGTC GTTCTTATTT GCTCCAGGGA CATTGTGGAG 
ACTCCGCCTA GTTCTGAAAT GTCTGTTTTT ACTAAAATTG GCTCAGCCAA ACCCATTGTA 
CTTCCAAAGA AAGGTAGTTT TGCTATGGTA GGATTTAAAG GGCTTTCCAC TCCATCCTGG 
ATTAAAATCA TTAATAACAG TGCAGATAGA AAAGCTGCAT CGATTCACCT TTATCTTCCA 
CTGATGTTGA CTGAATATAG ATGCCCGGCC CCAAAACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932301.4
CDS	252..4913
Translation	MCLDRRQRRSKVTGERGLNMLPTANMGCCAHGLLSPSSVDEDCLQIHLHRQWHGDTADTHNCDSCPAPTYQPGEKWSRHQDILTVTYLSGRGNIPMGKLAAFLPSSPSLSQVFLFLLMLLCWQRCAAGNLNFFPEDSINANMSVGNLGPDELSVVRNEAPSLSYVHGGNALTEGDPVFAPRYNITLHKKGKLWSCDSLLKLTRHILLTMLGPSLAFEVFPYHHCNQDLKPRDTLISPLRLLHAHVKPLLLHRVLIFLGELGFLVESQQAKALSVLKKQIFKTGKPMKEAGVKPKDNCPDKKPHLIPWNPGHDGSRSAVIGYGVALRLESSATFHSLLIQNGGSLVFADDLVPITLRAQYILIRNGGSFHIGSEKCPYRSNATITLYGKSTEGDNVEDFGKKFLGVDTGGILELHGRKPLSWTLLDATLHPGGLPHGPYKWEKQWGSRGINIRIIDPETGQVIAADRFDTHMFLDEGKRLEEFLAQQSPGKIVAAAVGDSAAKSLTPDTRKFLRDALSSKYIFHLGYRQPWALVGVLGEDLLMVAEDRKQYESNGTTGLATATREFQTYSGLHFTVTAYSGWIKGVPRNGFRVAVSRGIILTLLDDVTSWLPGNRIVVASTDYSMHQTEEFTLLPCPECRSNQVKISGSPLYLHIGEVIDGIDMRAEVGVLTRNVLIHGEMEDLCYGQNQCQFFNYDTFGGQVKIQGRFASVHLSGVELKNMGQQILGSYPVHFHMTGTVDEEGGYNPPTYVDNLSIHHCFSRCVVIHGTHGLLVKDTIGYDTLGHCFFLEDGVEQRNTFYHNLGLLTKAGTILPTDRSEAMCLAIRDSVYGDYVPVPSIDCMAVSTFWIANPNNNLIGNAAGGAQDVGIWFIFHRVPSGLCEGQYAEGHSEFTPLGVFYNNRVHSNFKAGLFIGKGVKTTVANAENPREYLTVDNARFRPHQDSDPTKPRIPAVIDGLIAFKNNDHGAWARGGDIIFRNSGFSDNGIGLTLASDGTFPTDEGSSLEITDSIFVGESENVGSQGGQNIYWGRGGLGEQRSFPRNKTFPVRGFQIYDGPVRLLKCTFKKFLPTLDRPVSAIGFSLKNSWQITPQNNVSLVRMEWNVGLKVFFGHSGQWFGSNNFDGDKVSIFHDLDGSVTGYPDTFIGRADNYLIRHPGCLTVPRWNGVICSGRYAQLYIQARRPENLNILVSRATYPSVPLKLSGVNRGAPYQQYQPVVMLHQSYIIRWEGQSPGEVILYPINFNRGDWIQIALCYPRSAQFQVMSDVYDRHSGRIHSVRHYSWAHTISQVLNQSETRLFHFSQESGYLFIHLQAHSVRSGHSYCSQHGCERIKIVSQFELEDSSECIQNPFPKTDSYTHTRRLFASHRLALPCSECGAPQVAITSNPSMKYTRIQIQSLRMADNVNGLKAAFIKVDEDIFLIKRKGLFFLVLDACSGAVVSKRQFEMISDLKITKAIASYIQTSMKLRSVVLICSRDIVETPPSSEMSVFTKIGSAKPIVLPKKGSFAMVGFKGLSTPSWIKIINNSADRKAASIHLYLPLMLTEYRCPAPKL

Target ORF information:

RefSeq Version	XM_002932255.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932255.5

ORF Insert Sequence:

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTGAGAGAGG TCTAAACATG 
CTCCCAACAG CCAATATGGG ATGCTGTGCA CATGGACTCT TGTCTCCTTC CTCTGTAGAT 
GAAGACTGTC TGCAGATCCA CTTACACAGG CAATGGCACG GGGACACTGC TGACACTCAT 
AATTGTGACT CATGCCCAGC ACCTACTTAT CAACCAGGAG AAAAGTGGAG CAGACACCAA 
GACATTCTCA CTGTCACCTA TCTCTCCGGG AGAGGAAACA TACCTATGGG AAAACTTGCA 
GCCTTTCTGC CTTCATCACC CAGCCTTTCA CAAGTGTTCC TATTCCTTCT GATGCTTCTG 
TGCTGGCAGA GATGTGCAGC TGGAAATCTA AACTTCTTTC CAGAAGACAG CATCAATGCC 
AACATGTCTG TAGGAAACCT GGGTCCTGAT GAGTTGAGTG TGGTTAGAAA TGAAGCTCCA 
TCACTTTCCT ATGTCCATGG GGGAAATGCA TTGACTGAAG GAGACCCAGT GTTTGCACCA 
AGATACAACA TAACTCTGCA CAAAAAGGGC AAGCTATGGT CATGTGACAG TCTTCTTAAG 
CTCACTAGAC ATATTCTATT GACTATGTTG GGGCCTAGTT TGGCTTTTGA AGTCTTCCCA 
TATCATCACT GTAACCAGGA CTTAAAGCCA AGGGACACAC TTATTAGTCC ACTGAGGCTG 
TTACACGCGC ACGTGAAACC ACTTCTACTT CACAGAGTTT TAATATTCCT GGGGGAGCTC 
GGATTTTTGG TTGAATCCCA ACAAGCTAAA GCCCTGTCGG TACTGAAGAA GCAGATCTTC 
AAAACAGGAA AACCAATGAA GGAGGCTGGA GTGAAACCAA AGGACAATTG CCCAGATAAG 
AAGCCACATC TGATCCCTTG GAACCCAGGA CATGATGGAA GCAGATCAGC TGTTATCGGT 
TATGGTGTTG CCCTACGATT GGAGTCATCA GCCACTTTCC ACTCACTGCT TATTCAGAAT 
GGAGGATCTT TAGTGTTTGC AGATGACCTA GTGCCAATTA CTTTGAGAGC ACAGTATATT 
TTAATCAGAA ATGGCGGATC TTTTCACATT GGCTCAGAAA AGTGTCCTTA TAGGTCCAAT 
GCTACCATCA CGCTTTATGG GAAGTCTACA GAAGGTGACA ATGTAGAAGA CTTTGGAAAG 
AAGTTCTTAG GAGTAGATAC AGGAGGGATT TTGGAATTAC ATGGAAGGAA ACCATTATCA 
TGGACTTTGT TAGATGCAAC CTTACATCCA GGAGGGCTCC CCCATGGACC ATATAAGTGG 
GAGAAGCAAT GGGGCAGCAG GGGCATTAAC ATACGAATCA TTGACCCTGA AACTGGTCAA 
GTAATAGCTG CTGATCGCTT TGACACCCAC ATGTTTCTGG ATGAAGGGAA AAGGCTGGAG 
GAGTTCCTTG CTCAACAGAG TCCAGGCAAG ATAGTGGCAG CAGCAGTGGG TGACTCAGCA 
GCCAAGAGCT TAACCCCAGA CACAAGGAAA TTTCTAAGAG ATGCTTTGAG CAGCAAATAT 
ATCTTTCACT TGGGCTACAG GCAGCCCTGG GCTTTAGTTG GGGTGCTTGG AGAAGACCTG 
TTGATGGTAG CAGAGGACAG AAAGCAATAT GAGAGCAATG GGACCACTGG TCTTGCAACT 
GCCACAAGGG AATTCCAGAC ATATTCGGGG CTGCACTTCA CTGTAACTGC TTACAGTGGC 
TGGATTAAAG GAGTTCCGCG AAATGGATTC AGAGTGGCAG TGTCGAGGGG CATCATCCTC 
ACATTACTGG ATGATGTTAC AAGTTGGTTG CCTGGTAACA GAATAGTCGT TGCTAGTACC 
GATTACTCAA TGCATCAGAC TGAAGAATTC ACCCTGCTTC CCTGCCCTGA ATGCAGAAGT 
AATCAAGTCA AAATTAGCGG TAGCCCACTT TATTTGCATA TTGGTGAAGT TATTGATGGA 
ATTGACATGA GAGCAGAGGT TGGGGTTCTC ACAAGAAATG TTCTTATCCA TGGTGAAATG 
GAAGATTTAT GTTATGGACA AAACCAGTGT CAGTTTTTCA ACTATGATAC CTTTGGAGGA 
CAAGTCAAGA TTCAAGGAAG ATTTGCATCA GTTCATCTGT CAGGAGTAGA ACTAAAAAAC 
ATGGGGCAGC AAATCTTGGG CAGTTACCCA GTTCATTTCC ACATGACAGG AACTGTAGAT 
GAGGAAGGGG GCTATAACCC TCCAACGTAT GTGGACAATC TTTCCATCCA CCACTGTTTC 
TCGAGATGTG TGGTGATTCA TGGGACCCAT GGACTTCTGG TGAAAGACAC GATTGGCTAT 
GACACACTGG GGCACTGTTT TTTTCTTGAG GATGGTGTTG AACAAAGAAA TACATTTTAT 
CACAATCTAG GATTGCTGAC AAAGGCTGGG ACCATCTTGC CCACAGACCG AAGTGAAGCC 
ATGTGTCTTG CTATCCGAGA TTCTGTCTAT GGAGATTATG TTCCTGTGCC TAGCATTGAC 
TGCATGGCTG TCAGCACATT TTGGATTGCA AACCCTAATA ACAATTTAAT AGGAAATGCT 
GCAGGAGGGG CACAGGATGT TGGCATATGG TTCATATTCC ACCGAGTTCC CTCTGGCCTT 
TGTGAAGGAC AGTATGCTGA AGGACATTCA GAATTTACCC CATTGGGGGT GTTTTACAAC 
AACAGGGTAC ACTCCAACTT CAAGGCCGGA TTATTCATTG GCAAAGGAGT CAAAACCACA 
GTAGCAAATG CTGAGAATCC AAGGGAATAT CTGACAGTAG ATAATGCCCG GTTTCGTCCC 
CATCAAGATT CAGATCCCAC CAAGCCTCGA ATACCAGCTG TTATTGATGG CCTAATAGCT 
TTCAAAAACA ATGACCATGG TGCATGGGCT AGAGGAGGGG ACATTATTTT CCGTAACTCA 
GGGTTTTCTG ATAATGGCAT TGGACTAACT TTAGCCAGTG ATGGGACATT TCCTACTGAT 
GAAGGCTCTA GCTTGGAGAT CACAGACTCC ATATTTGTGG GTGAGAGCGA GAATGTGGGA 
TCACAAGGTG GACAGAACAT CTACTGGGGA CGTGGGGGTT TGGGGGAGCA GCGATCATTT 
CCAAGAAACA AGACATTCCC AGTTCGTGGT TTCCAAATAT ACGATGGGCC CGTCAGGCTC 
CTGAAGTGCA CTTTCAAGAA ATTTCTCCCA ACTCTTGACA GACCTGTGAG CGCTATTGGG 
TTCTCCTTGA AAAATTCTTG GCAGATAACT CCACAGAACA ATGTGTCCCT GGTCAGGATG 
GAATGGAATG TTGGGCTGAA GGTTTTCTTT GGTCATTCAG GACAATGGTT TGGCTCAAAT 
AACTTTGATG GTGATAAAGT GTCAATCTTC CATGATTTGG ATGGCTCAGT GACTGGATAT 
CCTGATACCT TTATAGGAAG GGCAGATAAT TACCTGATCC GTCACCCTGG ATGCCTCACT 
GTGCCACGGT GGAACGGAGT GATTTGTTCA GGCAGGTATG CCCAGCTTTA CATCCAGGCC 
AGACGCCCTG AGAATCTGAA CATTTTGGTC TCTAGAGCAA CTTATCCCTC TGTTCCTTTA 
AAACTTTCTG GCGTTAACCG GGGTGCACCA TATCAACAGT ACCAACCAGT GGTCATGCTG 
CATCAATCCT ATATTATCAG ATGGGAAGGT CAGTCTCCTG GAGAGGTCAT CCTATACCCC 
ATCAACTTTA ACAGGGGAGA CTGGATTCAG ATTGCCTTGT GTTATCCCAG AAGTGCACAG 
TTCCAGGTCA TGTCAGATGT TTATGATCGT CACAGTGGAA GGATACACAG CGTAAGACAC 
TATTCCTGGG CTCATACCAT CAGCCAGGTT CTTAATCAGT CAGAGACAAG ACTTTTTCAT 
TTCAGTCAGG AATCTGGGTA CTTGTTCATA CACCTACAGG CACACTCAGT CCGCAGTGGG 
CACAGCTATT GTTCCCAGCA TGGCTGTGAG CGAATTAAGA TAGTGTCTCA ATTTGAACTA 
GAGGATTCCA GTGAATGTAT TCAGAACCCA TTTCCTAAAA CAGATAGTTA TACCCATACC 
CGCCGACTCT TTGCATCTCA CCGACTTGCC CTGCCATGCA GTGAATGTGG TGCTCCACAG 
GTAGCAATAA CAAGCAACCC ATCTATGAAA TATACAAGAA TACAGATCCA ATCCCTCCGT 
ATGGCAGATA ATGTAAATGG ACTGAAAGCT GCATTTATCA AGGTTGATGA AGACATTTTT 
TTGATCAAGA GAAAGGGGCT TTTCTTCCTG GTGTTAGATG CCTGCTCTGG AGCTGTTGTA 
AGCAAGAGGC AATTTGAAAT GATCAGTGAT TTGAAGATAA CAAAAGCAAT TGCCAGTTAC 
ATTCAGACCT CAATGAAACT AAGATCAGTC GTTCTTATTT GCTCCAGGGA CATTGTGGAG 
ACTCCGCCTA GTTCTGAAAT GTCTGTTTTT ACTAAAATTG GCTCAGCCAA ACCCATTGTA 
CTTCCAAAGA AAGGTAGTTT TGCTATGGTA GGATTTAAAG GGCTTTCCAC TCCATCCTGG 
ATTAAAATCA TTAATAACAG TGCAGATAGA AAAGCTGCAT CGATTCACCT TTATCTTCCA 
CTGATGTTGA CTGAATATAG ATGCCCGGCC CCAAAACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100491930 ( XM_012959543.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814997.2 Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X2, mRNA.

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CloneID	OXa55178
Clone ID Related Accession (Same CDS sequence)	XM_012959543.3
Accession Version	XM_012959543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cell surface hyaluronidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTCTAAACAT GCTCCCAACA 
GCCAATATGG GATGCTGTGC ACATGGACTC TTGTCTCCTT CCTCTGTAGA TGAAGACTGT 
CTGCAGATCC ACTTACACAG GCAATGGCAC GGGGACACTG CTGACACTCA TAATTGTGAC 
TCATGCCCAG CACCTACTTA TCAACCAGGA GAAAAGTGGA GCAGACACCA AGACATTCTC 
ACTGTCACCT ATCTCTCCGG GAGAGGAAAC ATACCTATGG GAAAACTTGC AGCCTTTCTG 
CCTTCATCAC CCAGCCTTTC ACAAGTGTTC CTATTCCTTC TGATGCTTCT GTGCTGGCAG 
AGATGTGCAG CTGGAAATCT AAACTTCTTT CCAGAAGACA GCATCAATGC CAACATGTCT 
GTAGGAAACC TGGGTCCTGA TGAGTTGAGT GTGGTTAGAA ATGAAGCTCC ATCACTTTCC 
TATGTCCATG GGGGAAATGC ATTGACTGAA GGAGACCCAG TGTTTGCACC AAGATACAAC 
ATAACTCTGC ACAAAAAGGG CAAGCTATGG TCATGTGACA GTCTTCTTAA GCTCACTAGA 
CATATTCTAT TGACTATGTT GGGGCCTAGT TTGGCTTTTG AAGTCTTCCC ATATCATCAC 
TGTAACCAGG ACTTAAAGCC AAGGGACACA CTTATTAGTC CACTGAGGCT GTTACACGCG 
CACGTGAAAC CACTTCTACT TCACAGAGTT TTAATATTCC TGGGGGAGCT CGGATTTTTG 
GTTGAATCCC AACAAGCTAA AGCCCTGTCG GTACTGAAGA AGCAGATCTT CAAAACAGGA 
AAACCAATGA AGGAGGCTGG AGTGAAACCA AAGGACAATT GCCCAGATAA GAAGCCACAT 
CTGATCCCTT GGAACCCAGG ACATGATGGA AGCAGATCAG CTGTTATCGG TTATGGTGTT 
GCCCTACGAT TGGAGTCATC AGCCACTTTC CACTCACTGC TTATTCAGAA TGGAGGATCT 
TTAGTGTTTG CAGATGACCT AGTGCCAATT ACTTTGAGAG CACAGTATAT TTTAATCAGA 
AATGGCGGAT CTTTTCACAT TGGCTCAGAA AAGTGTCCTT ATAGGTCCAA TGCTACCATC 
ACGCTTTATG GGAAGTCTAC AGAAGGTGAC AATGTAGAAG ACTTTGGAAA GAAGTTCTTA 
GGAGTAGATA CAGGAGGGAT TTTGGAATTA CATGGAAGGA AACCATTATC ATGGACTTTG 
TTAGATGCAA CCTTACATCC AGGAGGGCTC CCCCATGGAC CATATAAGTG GGAGAAGCAA 
TGGGGCAGCA GGGGCATTAA CATACGAATC ATTGACCCTG AAACTGGTCA AGTAATAGCT 
GCTGATCGCT TTGACACCCA CATGTTTCTG GATGAAGGGA AAAGGCTGGA GGAGTTCCTT 
GCTCAACAGA GTCCAGGCAA GATAGTGGCA GCAGCAGTGG GTGACTCAGC AGCCAAGAGC 
TTAACCCCAG ACACAAGGAA ATTTCTAAGA GATGCTTTGA GCAGCAAATA TATCTTTCAC 
TTGGGCTACA GGCAGCCCTG GGCTTTAGTT GGGGTGCTTG GAGAAGACCT GTTGATGGTA 
GCAGAGGACA GAAAGCAATA TGAGAGCAAT GGGACCACTG GTCTTGCAAC TGCCACAAGG 
GAATTCCAGA CATATTCGGG GCTGCACTTC ACTGTAACTG CTTACAGTGG CTGGATTAAA 
GGAGTTCCGC GAAATGGATT CAGAGTGGCA GTGTCGAGGG GCATCATCCT CACATTACTG 
GATGATGTTA CAAGTTGGTT GCCTGGTAAC AGAATAGTCG TTGCTAGTAC CGATTACTCA 
ATGCATCAGA CTGAAGAATT CACCCTGCTT CCCTGCCCTG AATGCAGAAG TAATCAAGTC 
AAAATTAGCG GTAGCCCACT TTATTTGCAT ATTGGTGAAG TTATTGATGG AATTGACATG 
AGAGCAGAGG TTGGGGTTCT CACAAGAAAT GTTCTTATCC ATGGTGAAAT GGAAGATTTA 
TGTTATGGAC AAAACCAGTG TCAGTTTTTC AACTATGATA CCTTTGGAGG ACAAGTCAAG 
ATTCAAGGAA GATTTGCATC AGTTCATCTG TCAGGAGTAG AACTAAAAAA CATGGGGCAG 
CAAATCTTGG GCAGTTACCC AGTTCATTTC CACATGACAG GAACTGTAGA TGAGGAAGGG 
GGCTATAACC CTCCAACGTA TGTGGACAAT CTTTCCATCC ACCACTGTTT CTCGAGATGT 
GTGGTGATTC ATGGGACCCA TGGACTTCTG GTGAAAGACA CGATTGGCTA TGACACACTG 
GGGCACTGTT TTTTTCTTGA GGATGGTGTT GAACAAAGAA ATACATTTTA TCACAATCTA 
GGATTGCTGA CAAAGGCTGG GACCATCTTG CCCACAGACC GAAGTGAAGC CATGTGTCTT 
GCTATCCGAG ATTCTGTCTA TGGAGATTAT GTTCCTGTGC CTAGCATTGA CTGCATGGCT 
GTCAGCACAT TTTGGATTGC AAACCCTAAT AACAATTTAA TAGGAAATGC TGCAGGAGGG 
GCACAGGATG TTGGCATATG GTTCATATTC CACCGAGTTC CCTCTGGCCT TTGTGAAGGA 
CAGTATGCTG AAGGACATTC AGAATTTACC CCATTGGGGG TGTTTTACAA CAACAGGGTA 
CACTCCAACT TCAAGGCCGG ATTATTCATT GGCAAAGGAG TCAAAACCAC AGTAGCAAAT 
GCTGAGAATC CAAGGGAATA TCTGACAGTA GATAATGCCC GGTTTCGTCC CCATCAAGAT 
TCAGATCCCA CCAAGCCTCG AATACCAGCT GTTATTGATG GCCTAATAGC TTTCAAAAAC 
AATGACCATG GTGCATGGGC TAGAGGAGGG GACATTATTT TCCGTAACTC AGGGTTTTCT 
GATAATGGCA TTGGACTAAC TTTAGCCAGT GATGGGACAT TTCCTACTGA TGAAGGCTCT 
AGCTTGGAGA TCACAGACTC CATATTTGTG GGTGAGAGCG AGAATGTGGG ATCACAAGGT 
GGACAGAACA TCTACTGGGG ACGTGGGGGT TTGGGGGAGC AGCGATCATT TCCAAGAAAC 
AAGACATTCC CAGTTCGTGG TTTCCAAATA TACGATGGGC CCGTCAGGCT CCTGAAGTGC 
ACTTTCAAGA AATTTCTCCC AACTCTTGAC AGACCTGTGA GCGCTATTGG GTTCTCCTTG 
AAAAATTCTT GGCAGATAAC TCCACAGAAC AATGTGTCCC TGGTCAGGAT GGAATGGAAT 
GTTGGGCTGA AGGTTTTCTT TGGTCATTCA GGACAATGGT TTGGCTCAAA TAACTTTGAT 
GGTGATAAAG TGTCAATCTT CCATGATTTG GATGGCTCAG TGACTGGATA TCCTGATACC 
TTTATAGGAA GGGCAGATAA TTACCTGATC CGTCACCCTG GATGCCTCAC TGTGCCACGG 
TGGAACGGAG TGATTTGTTC AGGCAGGTAT GCCCAGCTTT ACATCCAGGC CAGACGCCCT 
GAGAATCTGA ACATTTTGGT CTCTAGAGCA ACTTATCCCT CTGTTCCTTT AAAACTTTCT 
GGCGTTAACC GGGGTGCACC ATATCAACAG TACCAACCAG TGGTCATGCT GCATCAATCC 
TATATTATCA GATGGGAAGG TCAGTCTCCT GGAGAGGTCA TCCTATACCC CATCAACTTT 
AACAGGGGAG ACTGGATTCA GATTGCCTTG TGTTATCCCA GAAGTGCACA GTTCCAGGTC 
ATGTCAGATG TTTATGATCG TCACAGTGGA AGGATACACA GCGTAAGACA CTATTCCTGG 
GCTCATACCA TCAGCCAGGT TCTTAATCAG TCAGAGACAA GACTTTTTCA TTTCAGTCAG 
GAATCTGGGT ACTTGTTCAT ACACCTACAG GCACACTCAG TCCGCAGTGG GCACAGCTAT 
TGTTCCCAGC ATGGCTGTGA GCGAATTAAG ATAGTGTCTC AATTTGAACT AGAGGATTCC 
AGTGAATGTA TTCAGAACCC ATTTCCTAAA ACAGATAGTT ATACCCATAC CCGCCGACTC 
TTTGCATCTC ACCGACTTGC CCTGCCATGC AGTGAATGTG GTGCTCCACA GGTAGCAATA 
ACAAGCAACC CATCTATGAA ATATACAAGA ATACAGATCC AATCCCTCCG TATGGCAGAT 
AATGTAAATG GACTGAAAGC TGCATTTATC AAGGTTGATG AAGACATTTT TTTGATCAAG 
AGAAAGGGGC TTTTCTTCCT GGTGTTAGAT GCCTGCTCTG GAGCTGTTGT AAGCAAGAGG 
CAATTTGAAA TGATCAGTGA TTTGAAGATA ACAAAAGCAA TTGCCAGTTA CATTCAGACC 
TCAATGAAAC TAAGATCAGT CGTTCTTATT TGCTCCAGGG ACATTGTGGA GACTCCGCCT 
AGTTCTGAAA TGTCTGTTTT TACTAAAATT GGCTCAGCCA AACCCATTGT ACTTCCAAAG 
AAAGGTAGTT TTGCTATGGT AGGATTTAAA GGGCTTTCCA CTCCATCCTG GATTAAAATC 
ATTAATAACA GTGCAGATAG AAAAGCTGCA TCGATTCACC TTTATCTTCC ACTGATGTTG 
ACTGAATATA GATGCCCGGC CCCAAAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814997.2
CDS	252..4904
Translation	MCLDRRQRRSKVTGLNMLPTANMGCCAHGLLSPSSVDEDCLQIHLHRQWHGDTADTHNCDSCPAPTYQPGEKWSRHQDILTVTYLSGRGNIPMGKLAAFLPSSPSLSQVFLFLLMLLCWQRCAAGNLNFFPEDSINANMSVGNLGPDELSVVRNEAPSLSYVHGGNALTEGDPVFAPRYNITLHKKGKLWSCDSLLKLTRHILLTMLGPSLAFEVFPYHHCNQDLKPRDTLISPLRLLHAHVKPLLLHRVLIFLGELGFLVESQQAKALSVLKKQIFKTGKPMKEAGVKPKDNCPDKKPHLIPWNPGHDGSRSAVIGYGVALRLESSATFHSLLIQNGGSLVFADDLVPITLRAQYILIRNGGSFHIGSEKCPYRSNATITLYGKSTEGDNVEDFGKKFLGVDTGGILELHGRKPLSWTLLDATLHPGGLPHGPYKWEKQWGSRGINIRIIDPETGQVIAADRFDTHMFLDEGKRLEEFLAQQSPGKIVAAAVGDSAAKSLTPDTRKFLRDALSSKYIFHLGYRQPWALVGVLGEDLLMVAEDRKQYESNGTTGLATATREFQTYSGLHFTVTAYSGWIKGVPRNGFRVAVSRGIILTLLDDVTSWLPGNRIVVASTDYSMHQTEEFTLLPCPECRSNQVKISGSPLYLHIGEVIDGIDMRAEVGVLTRNVLIHGEMEDLCYGQNQCQFFNYDTFGGQVKIQGRFASVHLSGVELKNMGQQILGSYPVHFHMTGTVDEEGGYNPPTYVDNLSIHHCFSRCVVIHGTHGLLVKDTIGYDTLGHCFFLEDGVEQRNTFYHNLGLLTKAGTILPTDRSEAMCLAIRDSVYGDYVPVPSIDCMAVSTFWIANPNNNLIGNAAGGAQDVGIWFIFHRVPSGLCEGQYAEGHSEFTPLGVFYNNRVHSNFKAGLFIGKGVKTTVANAENPREYLTVDNARFRPHQDSDPTKPRIPAVIDGLIAFKNNDHGAWARGGDIIFRNSGFSDNGIGLTLASDGTFPTDEGSSLEITDSIFVGESENVGSQGGQNIYWGRGGLGEQRSFPRNKTFPVRGFQIYDGPVRLLKCTFKKFLPTLDRPVSAIGFSLKNSWQITPQNNVSLVRMEWNVGLKVFFGHSGQWFGSNNFDGDKVSIFHDLDGSVTGYPDTFIGRADNYLIRHPGCLTVPRWNGVICSGRYAQLYIQARRPENLNILVSRATYPSVPLKLSGVNRGAPYQQYQPVVMLHQSYIIRWEGQSPGEVILYPINFNRGDWIQIALCYPRSAQFQVMSDVYDRHSGRIHSVRHYSWAHTISQVLNQSETRLFHFSQESGYLFIHLQAHSVRSGHSYCSQHGCERIKIVSQFELEDSSECIQNPFPKTDSYTHTRRLFASHRLALPCSECGAPQVAITSNPSMKYTRIQIQSLRMADNVNGLKAAFIKVDEDIFLIKRKGLFFLVLDACSGAVVSKRQFEMISDLKITKAIASYIQTSMKLRSVVLICSRDIVETPPSSEMSVFTKIGSAKPIVLPKKGSFAMVGFKGLSTPSWIKIINNSADRKAASIHLYLPLMLTEYRCPAPKL

Target ORF information:

RefSeq Version	XM_012959543.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959543.3

ORF Insert Sequence:

ATGTGCTTGG ACCGGCGGCA AAGGAGGAGC AAAGTGACCG GTCTAAACAT GCTCCCAACA 
GCCAATATGG GATGCTGTGC ACATGGACTC TTGTCTCCTT CCTCTGTAGA TGAAGACTGT 
CTGCAGATCC ACTTACACAG GCAATGGCAC GGGGACACTG CTGACACTCA TAATTGTGAC 
TCATGCCCAG CACCTACTTA TCAACCAGGA GAAAAGTGGA GCAGACACCA AGACATTCTC 
ACTGTCACCT ATCTCTCCGG GAGAGGAAAC ATACCTATGG GAAAACTTGC AGCCTTTCTG 
CCTTCATCAC CCAGCCTTTC ACAAGTGTTC CTATTCCTTC TGATGCTTCT GTGCTGGCAG 
AGATGTGCAG CTGGAAATCT AAACTTCTTT CCAGAAGACA GCATCAATGC CAACATGTCT 
GTAGGAAACC TGGGTCCTGA TGAGTTGAGT GTGGTTAGAA ATGAAGCTCC ATCACTTTCC 
TATGTCCATG GGGGAAATGC ATTGACTGAA GGAGACCCAG TGTTTGCACC AAGATACAAC 
ATAACTCTGC ACAAAAAGGG CAAGCTATGG TCATGTGACA GTCTTCTTAA GCTCACTAGA 
CATATTCTAT TGACTATGTT GGGGCCTAGT TTGGCTTTTG AAGTCTTCCC ATATCATCAC 
TGTAACCAGG ACTTAAAGCC AAGGGACACA CTTATTAGTC CACTGAGGCT GTTACACGCG 
CACGTGAAAC CACTTCTACT TCACAGAGTT TTAATATTCC TGGGGGAGCT CGGATTTTTG 
GTTGAATCCC AACAAGCTAA AGCCCTGTCG GTACTGAAGA AGCAGATCTT CAAAACAGGA 
AAACCAATGA AGGAGGCTGG AGTGAAACCA AAGGACAATT GCCCAGATAA GAAGCCACAT 
CTGATCCCTT GGAACCCAGG ACATGATGGA AGCAGATCAG CTGTTATCGG TTATGGTGTT 
GCCCTACGAT TGGAGTCATC AGCCACTTTC CACTCACTGC TTATTCAGAA TGGAGGATCT 
TTAGTGTTTG CAGATGACCT AGTGCCAATT ACTTTGAGAG CACAGTATAT TTTAATCAGA 
AATGGCGGAT CTTTTCACAT TGGCTCAGAA AAGTGTCCTT ATAGGTCCAA TGCTACCATC 
ACGCTTTATG GGAAGTCTAC AGAAGGTGAC AATGTAGAAG ACTTTGGAAA GAAGTTCTTA 
GGAGTAGATA CAGGAGGGAT TTTGGAATTA CATGGAAGGA AACCATTATC ATGGACTTTG 
TTAGATGCAA CCTTACATCC AGGAGGGCTC CCCCATGGAC CATATAAGTG GGAGAAGCAA 
TGGGGCAGCA GGGGCATTAA CATACGAATC ATTGACCCTG AAACTGGTCA AGTAATAGCT 
GCTGATCGCT TTGACACCCA CATGTTTCTG GATGAAGGGA AAAGGCTGGA GGAGTTCCTT 
GCTCAACAGA GTCCAGGCAA GATAGTGGCA GCAGCAGTGG GTGACTCAGC AGCCAAGAGC 
TTAACCCCAG ACACAAGGAA ATTTCTAAGA GATGCTTTGA GCAGCAAATA TATCTTTCAC 
TTGGGCTACA GGCAGCCCTG GGCTTTAGTT GGGGTGCTTG GAGAAGACCT GTTGATGGTA 
GCAGAGGACA GAAAGCAATA TGAGAGCAAT GGGACCACTG GTCTTGCAAC TGCCACAAGG 
GAATTCCAGA CATATTCGGG GCTGCACTTC ACTGTAACTG CTTACAGTGG CTGGATTAAA 
GGAGTTCCGC GAAATGGATT CAGAGTGGCA GTGTCGAGGG GCATCATCCT CACATTACTG 
GATGATGTTA CAAGTTGGTT GCCTGGTAAC AGAATAGTCG TTGCTAGTAC CGATTACTCA 
ATGCATCAGA CTGAAGAATT CACCCTGCTT CCCTGCCCTG AATGCAGAAG TAATCAAGTC 
AAAATTAGCG GTAGCCCACT TTATTTGCAT ATTGGTGAAG TTATTGATGG AATTGACATG 
AGAGCAGAGG TTGGGGTTCT CACAAGAAAT GTTCTTATCC ATGGTGAAAT GGAAGATTTA 
TGTTATGGAC AAAACCAGTG TCAGTTTTTC AACTATGATA CCTTTGGAGG ACAAGTCAAG 
ATTCAAGGAA GATTTGCATC AGTTCATCTG TCAGGAGTAG AACTAAAAAA CATGGGGCAG 
CAAATCTTGG GCAGTTACCC AGTTCATTTC CACATGACAG GAACTGTAGA TGAGGAAGGG 
GGCTATAACC CTCCAACGTA TGTGGACAAT CTTTCCATCC ACCACTGTTT CTCGAGATGT 
GTGGTGATTC ATGGGACCCA TGGACTTCTG GTGAAAGACA CGATTGGCTA TGACACACTG 
GGGCACTGTT TTTTTCTTGA GGATGGTGTT GAACAAAGAA ATACATTTTA TCACAATCTA 
GGATTGCTGA CAAAGGCTGG GACCATCTTG CCCACAGACC GAAGTGAAGC CATGTGTCTT 
GCTATCCGAG ATTCTGTCTA TGGAGATTAT GTTCCTGTGC CTAGCATTGA CTGCATGGCT 
GTCAGCACAT TTTGGATTGC AAACCCTAAT AACAATTTAA TAGGAAATGC TGCAGGAGGG 
GCACAGGATG TTGGCATATG GTTCATATTC CACCGAGTTC CCTCTGGCCT TTGTGAAGGA 
CAGTATGCTG AAGGACATTC AGAATTTACC CCATTGGGGG TGTTTTACAA CAACAGGGTA 
CACTCCAACT TCAAGGCCGG ATTATTCATT GGCAAAGGAG TCAAAACCAC AGTAGCAAAT 
GCTGAGAATC CAAGGGAATA TCTGACAGTA GATAATGCCC GGTTTCGTCC CCATCAAGAT 
TCAGATCCCA CCAAGCCTCG AATACCAGCT GTTATTGATG GCCTAATAGC TTTCAAAAAC 
AATGACCATG GTGCATGGGC TAGAGGAGGG GACATTATTT TCCGTAACTC AGGGTTTTCT 
GATAATGGCA TTGGACTAAC TTTAGCCAGT GATGGGACAT TTCCTACTGA TGAAGGCTCT 
AGCTTGGAGA TCACAGACTC CATATTTGTG GGTGAGAGCG AGAATGTGGG ATCACAAGGT 
GGACAGAACA TCTACTGGGG ACGTGGGGGT TTGGGGGAGC AGCGATCATT TCCAAGAAAC 
AAGACATTCC CAGTTCGTGG TTTCCAAATA TACGATGGGC CCGTCAGGCT CCTGAAGTGC 
ACTTTCAAGA AATTTCTCCC AACTCTTGAC AGACCTGTGA GCGCTATTGG GTTCTCCTTG 
AAAAATTCTT GGCAGATAAC TCCACAGAAC AATGTGTCCC TGGTCAGGAT GGAATGGAAT 
GTTGGGCTGA AGGTTTTCTT TGGTCATTCA GGACAATGGT TTGGCTCAAA TAACTTTGAT 
GGTGATAAAG TGTCAATCTT CCATGATTTG GATGGCTCAG TGACTGGATA TCCTGATACC 
TTTATAGGAA GGGCAGATAA TTACCTGATC CGTCACCCTG GATGCCTCAC TGTGCCACGG 
TGGAACGGAG TGATTTGTTC AGGCAGGTAT GCCCAGCTTT ACATCCAGGC CAGACGCCCT 
GAGAATCTGA ACATTTTGGT CTCTAGAGCA ACTTATCCCT CTGTTCCTTT AAAACTTTCT 
GGCGTTAACC GGGGTGCACC ATATCAACAG TACCAACCAG TGGTCATGCT GCATCAATCC 
TATATTATCA GATGGGAAGG TCAGTCTCCT GGAGAGGTCA TCCTATACCC CATCAACTTT 
AACAGGGGAG ACTGGATTCA GATTGCCTTG TGTTATCCCA GAAGTGCACA GTTCCAGGTC 
ATGTCAGATG TTTATGATCG TCACAGTGGA AGGATACACA GCGTAAGACA CTATTCCTGG 
GCTCATACCA TCAGCCAGGT TCTTAATCAG TCAGAGACAA GACTTTTTCA TTTCAGTCAG 
GAATCTGGGT ACTTGTTCAT ACACCTACAG GCACACTCAG TCCGCAGTGG GCACAGCTAT 
TGTTCCCAGC ATGGCTGTGA GCGAATTAAG ATAGTGTCTC AATTTGAACT AGAGGATTCC 
AGTGAATGTA TTCAGAACCC ATTTCCTAAA ACAGATAGTT ATACCCATAC CCGCCGACTC 
TTTGCATCTC ACCGACTTGC CCTGCCATGC AGTGAATGTG GTGCTCCACA GGTAGCAATA 
ACAAGCAACC CATCTATGAA ATATACAAGA ATACAGATCC AATCCCTCCG TATGGCAGAT 
AATGTAAATG GACTGAAAGC TGCATTTATC AAGGTTGATG AAGACATTTT TTTGATCAAG 
AGAAAGGGGC TTTTCTTCCT GGTGTTAGAT GCCTGCTCTG GAGCTGTTGT AAGCAAGAGG 
CAATTTGAAA TGATCAGTGA TTTGAAGATA ACAAAAGCAA TTGCCAGTTA CATTCAGACC 
TCAATGAAAC TAAGATCAGT CGTTCTTATT TGCTCCAGGG ACATTGTGGA GACTCCGCCT 
AGTTCTGAAA TGTCTGTTTT TACTAAAATT GGCTCAGCCA AACCCATTGT ACTTCCAAAG 
AAAGGTAGTT TTGCTATGGT AGGATTTAAA GGGCTTTCCA CTCCATCCTG GATTAAAATC 
ATTAATAACA GTGCAGATAG AAAAGCTGCA TCGATTCACC TTTATCTTCC ACTGATGTTG 
ACTGAATATA GATGCCCGGC CCCAAAACTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100491930 ( XM_031899223.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755083.1 Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X3, mRNA.

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CloneID	OXa55179
Clone ID Related Accession (Same CDS sequence)	XM_031899223.1
Accession Version	XM_031899223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cell surface hyaluronidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCCAA CAGCCAATAT GGGATGCTGT GCACATGGAC TCTTGTCTCC TTCCTCTGTA 
GATGAAGACT GTCTGCAGAT CCACTTACAC AGGCAATGGC ACGGGGACAC TGCTGACACT 
CATAATTGTG ACTCATGCCC AGCACCTACT TATCAACCAG GAGAAAAGTG GAGCAGACAC 
CAAGACATTC TCACTGTCAC CTATCTCTCC GGGAGAGGAA ACATACCTAT GGGAAAACTT 
GCAGCCTTTC TGCCTTCATC ACCCAGCCTT TCACAAGTGT TCCTATTCCT TCTGATGCTT 
CTGTGCTGGC AGAGATGTGC AGCTGGAAAT CTAAACTTCT TTCCAGAAGA CAGCATCAAT 
GCCAACATGT CTGTAGGAAA CCTGGGTCCT GATGAGTTGA GTGTGGTTAG AAATGAAGCT 
CCATCACTTT CCTATGTCCA TGGGGGAAAT GCATTGACTG AAGGAGACCC AGTGTTTGCA 
CCAAGATACA ACATAACTCT GCACAAAAAG GGCAAGCTAT GGTCATGTGA CAGTCTTCTT 
AAGCTCACTA GACATATTCT ATTGACTATG TTGGGGCCTA GTTTGGCTTT TGAAGTCTTC 
CCATATCATC ACTGTAACCA GGACTTAAAG CCAAGGGACA CACTTATTAG TCCACTGAGG 
CTGTTACACG CGCACGTGAA ACCACTTCTA CTTCACAGAG TTTTAATATT CCTGGGGGAG 
CTCGGATTTT TGGTTGAATC CCAACAAGCT AAAGCCCTGT CGGTACTGAA GAAGCAGATC 
TTCAAAACAG GAAAACCAAT GAAGGAGGCT GGAGTGAAAC CAAAGGACAA TTGCCCAGAT 
AAGAAGCCAC ATCTGATCCC TTGGAACCCA GGACATGATG GAAGCAGATC AGCTGTTATC 
GGTTATGGTG TTGCCCTACG ATTGGAGTCA TCAGCCACTT TCCACTCACT GCTTATTCAG 
AATGGAGGAT CTTTAGTGTT TGCAGATGAC CTAGTGCCAA TTACTTTGAG AGCACAGTAT 
ATTTTAATCA GAAATGGCGG ATCTTTTCAC ATTGGCTCAG AAAAGTGTCC TTATAGGTCC 
AATGCTACCA TCACGCTTTA TGGGAAGTCT ACAGAAGGTG ACAATGTAGA AGACTTTGGA 
AAGAAGTTCT TAGGAGTAGA TACAGGAGGG ATTTTGGAAT TACATGGAAG GAAACCATTA 
TCATGGACTT TGTTAGATGC AACCTTACAT CCAGGAGGGC TCCCCCATGG ACCATATAAG 
TGGGAGAAGC AATGGGGCAG CAGGGGCATT AACATACGAA TCATTGACCC TGAAACTGGT 
CAAGTAATAG CTGCTGATCG CTTTGACACC CACATGTTTC TGGATGAAGG GAAAAGGCTG 
GAGGAGTTCC TTGCTCAACA GAGTCCAGGC AAGATAGTGG CAGCAGCAGT GGGTGACTCA 
GCAGCCAAGA GCTTAACCCC AGACACAAGG AAATTTCTAA GAGATGCTTT GAGCAGCAAA 
TATATCTTTC ACTTGGGCTA CAGGCAGCCC TGGGCTTTAG TTGGGGTGCT TGGAGAAGAC 
CTGTTGATGG TAGCAGAGGA CAGAAAGCAA TATGAGAGCA ATGGGACCAC TGGTCTTGCA 
ACTGCCACAA GGGAATTCCA GACATATTCG GGGCTGCACT TCACTGTAAC TGCTTACAGT 
GGCTGGATTA AAGGAGTTCC GCGAAATGGA TTCAGAGTGG CAGTGTCGAG GGGCATCATC 
CTCACATTAC TGGATGATGT TACAAGTTGG TTGCCTGGTA ACAGAATAGT CGTTGCTAGT 
ACCGATTACT CAATGCATCA GACTGAAGAA TTCACCCTGC TTCCCTGCCC TGAATGCAGA 
AGTAATCAAG TCAAAATTAG CGGTAGCCCA CTTTATTTGC ATATTGGTGA AGTTATTGAT 
GGAATTGACA TGAGAGCAGA GGTTGGGGTT CTCACAAGAA ATGTTCTTAT CCATGGTGAA 
ATGGAAGATT TATGTTATGG ACAAAACCAG TGTCAGTTTT TCAACTATGA TACCTTTGGA 
GGACAAGTCA AGATTCAAGG AAGATTTGCA TCAGTTCATC TGTCAGGAGT AGAACTAAAA 
AACATGGGGC AGCAAATCTT GGGCAGTTAC CCAGTTCATT TCCACATGAC AGGAACTGTA 
GATGAGGAAG GGGGCTATAA CCCTCCAACG TATGTGGACA ATCTTTCCAT CCACCACTGT 
TTCTCGAGAT GTGTGGTGAT TCATGGGACC CATGGACTTC TGGTGAAAGA CACGATTGGC 
TATGACACAC TGGGGCACTG TTTTTTTCTT GAGGATGGTG TTGAACAAAG AAATACATTT 
TATCACAATC TAGGATTGCT GACAAAGGCT GGGACCATCT TGCCCACAGA CCGAAGTGAA 
GCCATGTGTC TTGCTATCCG AGATTCTGTC TATGGAGATT ATGTTCCTGT GCCTAGCATT 
GACTGCATGG CTGTCAGCAC ATTTTGGATT GCAAACCCTA ATAACAATTT AATAGGAAAT 
GCTGCAGGAG GGGCACAGGA TGTTGGCATA TGGTTCATAT TCCACCGAGT TCCCTCTGGC 
CTTTGTGAAG GACAGTATGC TGAAGGACAT TCAGAATTTA CCCCATTGGG GGTGTTTTAC 
AACAACAGGG TACACTCCAA CTTCAAGGCC GGATTATTCA TTGGCAAAGG AGTCAAAACC 
ACAGTAGCAA ATGCTGAGAA TCCAAGGGAA TATCTGACAG TAGATAATGC CCGGTTTCGT 
CCCCATCAAG ATTCAGATCC CACCAAGCCT CGAATACCAG CTGTTATTGA TGGCCTAATA 
GCTTTCAAAA ACAATGACCA TGGTGCATGG GCTAGAGGAG GGGACATTAT TTTCCGTAAC 
TCAGGGTTTT CTGATAATGG CATTGGACTA ACTTTAGCCA GTGATGGGAC ATTTCCTACT 
GATGAAGGCT CTAGCTTGGA GATCACAGAC TCCATATTTG TGGGTGAGAG CGAGAATGTG 
GGATCACAAG GTGGACAGAA CATCTACTGG GGACGTGGGG GTTTGGGGGA GCAGCGATCA 
TTTCCAAGAA ACAAGACATT CCCAGTTCGT GGTTTCCAAA TATACGATGG GCCCGTCAGG 
CTCCTGAAGT GCACTTTCAA GAAATTTCTC CCAACTCTTG ACAGACCTGT GAGCGCTATT 
GGGTTCTCCT TGAAAAATTC TTGGCAGATA ACTCCACAGA ACAATGTGTC CCTGGTCAGG 
ATGGAATGGA ATGTTGGGCT GAAGGTTTTC TTTGGTCATT CAGGACAATG GTTTGGCTCA 
AATAACTTTG ATGGTGATAA AGTGTCAATC TTCCATGATT TGGATGGCTC AGTGACTGGA 
TATCCTGATA CCTTTATAGG AAGGGCAGAT AATTACCTGA TCCGTCACCC TGGATGCCTC 
ACTGTGCCAC GGTGGAACGG AGTGATTTGT TCAGGCAGGT ATGCCCAGCT TTACATCCAG 
GCCAGACGCC CTGAGAATCT GAACATTTTG GTCTCTAGAG CAACTTATCC CTCTGTTCCT 
TTAAAACTTT CTGGCGTTAA CCGGGGTGCA CCATATCAAC AGTACCAACC AGTGGTCATG 
CTGCATCAAT CCTATATTAT CAGATGGGAA GGTCAGTCTC CTGGAGAGGT CATCCTATAC 
CCCATCAACT TTAACAGGGG AGACTGGATT CAGATTGCCT TGTGTTATCC CAGAAGTGCA 
CAGTTCCAGG TCATGTCAGA TGTTTATGAT CGTCACAGTG GAAGGATACA CAGCGTAAGA 
CACTATTCCT GGGCTCATAC CATCAGCCAG GTTCTTAATC AGTCAGAGAC AAGACTTTTT 
CATTTCAGTC AGGAATCTGG GTACTTGTTC ATACACCTAC AGGCACACTC AGTCCGCAGT 
GGGCACAGCT ATTGTTCCCA GCATGGCTGT GAGCGAATTA AGATAGTGTC TCAATTTGAA 
CTAGAGGATT CCAGTGAATG TATTCAGAAC CCATTTCCTA AAACAGATAG TTATACCCAT 
ACCCGCCGAC TCTTTGCATC TCACCGACTT GCCCTGCCAT GCAGTGAATG TGGTGCTCCA 
CAGGTAGCAA TAACAAGCAA CCCATCTATG AAATATACAA GAATACAGAT CCAATCCCTC 
CGTATGGCAG ATAATGTAAA TGGACTGAAA GCTGCATTTA TCAAGGTTGA TGAAGACATT 
TTTTTGATCA AGAGAAAGGG GCTTTTCTTC CTGGTGTTAG ATGCCTGCTC TGGAGCTGTT 
GTAAGCAAGA GGCAATTTGA AATGATCAGT GATTTGAAGA TAACAAAAGC AATTGCCAGT 
TACATTCAGA CCTCAATGAA ACTAAGATCA GTCGTTCTTA TTTGCTCCAG GGACATTGTG 
GAGACTCCGC CTAGTTCTGA AATGTCTGTT TTTACTAAAA TTGGCTCAGC CAAACCCATT 
GTACTTCCAA AGAAAGGTAG TTTTGCTATG GTAGGATTTA AAGGGCTTTC CACTCCATCC 
TGGATTAAAA TCATTAATAA CAGTGCAGAT AGAAAAGCTG CATCGATTCA CCTTTATCTT 
CCACTGATGT TGACTGAATA TAGATGCCCG GCCCCAAAAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755083.1
CDS	347..4951
Translation	MLPTANMGCCAHGLLSPSSVDEDCLQIHLHRQWHGDTADTHNCDSCPAPTYQPGEKWSRHQDILTVTYLSGRGNIPMGKLAAFLPSSPSLSQVFLFLLMLLCWQRCAAGNLNFFPEDSINANMSVGNLGPDELSVVRNEAPSLSYVHGGNALTEGDPVFAPRYNITLHKKGKLWSCDSLLKLTRHILLTMLGPSLAFEVFPYHHCNQDLKPRDTLISPLRLLHAHVKPLLLHRVLIFLGELGFLVESQQAKALSVLKKQIFKTGKPMKEAGVKPKDNCPDKKPHLIPWNPGHDGSRSAVIGYGVALRLESSATFHSLLIQNGGSLVFADDLVPITLRAQYILIRNGGSFHIGSEKCPYRSNATITLYGKSTEGDNVEDFGKKFLGVDTGGILELHGRKPLSWTLLDATLHPGGLPHGPYKWEKQWGSRGINIRIIDPETGQVIAADRFDTHMFLDEGKRLEEFLAQQSPGKIVAAAVGDSAAKSLTPDTRKFLRDALSSKYIFHLGYRQPWALVGVLGEDLLMVAEDRKQYESNGTTGLATATREFQTYSGLHFTVTAYSGWIKGVPRNGFRVAVSRGIILTLLDDVTSWLPGNRIVVASTDYSMHQTEEFTLLPCPECRSNQVKISGSPLYLHIGEVIDGIDMRAEVGVLTRNVLIHGEMEDLCYGQNQCQFFNYDTFGGQVKIQGRFASVHLSGVELKNMGQQILGSYPVHFHMTGTVDEEGGYNPPTYVDNLSIHHCFSRCVVIHGTHGLLVKDTIGYDTLGHCFFLEDGVEQRNTFYHNLGLLTKAGTILPTDRSEAMCLAIRDSVYGDYVPVPSIDCMAVSTFWIANPNNNLIGNAAGGAQDVGIWFIFHRVPSGLCEGQYAEGHSEFTPLGVFYNNRVHSNFKAGLFIGKGVKTTVANAENPREYLTVDNARFRPHQDSDPTKPRIPAVIDGLIAFKNNDHGAWARGGDIIFRNSGFSDNGIGLTLASDGTFPTDEGSSLEITDSIFVGESENVGSQGGQNIYWGRGGLGEQRSFPRNKTFPVRGFQIYDGPVRLLKCTFKKFLPTLDRPVSAIGFSLKNSWQITPQNNVSLVRMEWNVGLKVFFGHSGQWFGSNNFDGDKVSIFHDLDGSVTGYPDTFIGRADNYLIRHPGCLTVPRWNGVICSGRYAQLYIQARRPENLNILVSRATYPSVPLKLSGVNRGAPYQQYQPVVMLHQSYIIRWEGQSPGEVILYPINFNRGDWIQIALCYPRSAQFQVMSDVYDRHSGRIHSVRHYSWAHTISQVLNQSETRLFHFSQESGYLFIHLQAHSVRSGHSYCSQHGCERIKIVSQFELEDSSECIQNPFPKTDSYTHTRRLFASHRLALPCSECGAPQVAITSNPSMKYTRIQIQSLRMADNVNGLKAAFIKVDEDIFLIKRKGLFFLVLDACSGAVVSKRQFEMISDLKITKAIASYIQTSMKLRSVVLICSRDIVETPPSSEMSVFTKIGSAKPIVLPKKGSFAMVGFKGLSTPSWIKIINNSADRKAASIHLYLPLMLTEYRCPAPKL

Target ORF information:

RefSeq Version	XM_031899223.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cell surface hyaluronidase (LOC100491930), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899223.1

ORF Insert Sequence:

ATGCTCCCAA CAGCCAATAT GGGATGCTGT GCACATGGAC TCTTGTCTCC TTCCTCTGTA 
GATGAAGACT GTCTGCAGAT CCACTTACAC AGGCAATGGC ACGGGGACAC TGCTGACACT 
CATAATTGTG ACTCATGCCC AGCACCTACT TATCAACCAG GAGAAAAGTG GAGCAGACAC 
CAAGACATTC TCACTGTCAC CTATCTCTCC GGGAGAGGAA ACATACCTAT GGGAAAACTT 
GCAGCCTTTC TGCCTTCATC ACCCAGCCTT TCACAAGTGT TCCTATTCCT TCTGATGCTT 
CTGTGCTGGC AGAGATGTGC AGCTGGAAAT CTAAACTTCT TTCCAGAAGA CAGCATCAAT 
GCCAACATGT CTGTAGGAAA CCTGGGTCCT GATGAGTTGA GTGTGGTTAG AAATGAAGCT 
CCATCACTTT CCTATGTCCA TGGGGGAAAT GCATTGACTG AAGGAGACCC AGTGTTTGCA 
CCAAGATACA ACATAACTCT GCACAAAAAG GGCAAGCTAT GGTCATGTGA CAGTCTTCTT 
AAGCTCACTA GACATATTCT ATTGACTATG TTGGGGCCTA GTTTGGCTTT TGAAGTCTTC 
CCATATCATC ACTGTAACCA GGACTTAAAG CCAAGGGACA CACTTATTAG TCCACTGAGG 
CTGTTACACG CGCACGTGAA ACCACTTCTA CTTCACAGAG TTTTAATATT CCTGGGGGAG 
CTCGGATTTT TGGTTGAATC CCAACAAGCT AAAGCCCTGT CGGTACTGAA GAAGCAGATC 
TTCAAAACAG GAAAACCAAT GAAGGAGGCT GGAGTGAAAC CAAAGGACAA TTGCCCAGAT 
AAGAAGCCAC ATCTGATCCC TTGGAACCCA GGACATGATG GAAGCAGATC AGCTGTTATC 
GGTTATGGTG TTGCCCTACG ATTGGAGTCA TCAGCCACTT TCCACTCACT GCTTATTCAG 
AATGGAGGAT CTTTAGTGTT TGCAGATGAC CTAGTGCCAA TTACTTTGAG AGCACAGTAT 
ATTTTAATCA GAAATGGCGG ATCTTTTCAC ATTGGCTCAG AAAAGTGTCC TTATAGGTCC 
AATGCTACCA TCACGCTTTA TGGGAAGTCT ACAGAAGGTG ACAATGTAGA AGACTTTGGA 
AAGAAGTTCT TAGGAGTAGA TACAGGAGGG ATTTTGGAAT TACATGGAAG GAAACCATTA 
TCATGGACTT TGTTAGATGC AACCTTACAT CCAGGAGGGC TCCCCCATGG ACCATATAAG 
TGGGAGAAGC AATGGGGCAG CAGGGGCATT AACATACGAA TCATTGACCC TGAAACTGGT 
CAAGTAATAG CTGCTGATCG CTTTGACACC CACATGTTTC TGGATGAAGG GAAAAGGCTG 
GAGGAGTTCC TTGCTCAACA GAGTCCAGGC AAGATAGTGG CAGCAGCAGT GGGTGACTCA 
GCAGCCAAGA GCTTAACCCC AGACACAAGG AAATTTCTAA GAGATGCTTT GAGCAGCAAA 
TATATCTTTC ACTTGGGCTA CAGGCAGCCC TGGGCTTTAG TTGGGGTGCT TGGAGAAGAC 
CTGTTGATGG TAGCAGAGGA CAGAAAGCAA TATGAGAGCA ATGGGACCAC TGGTCTTGCA 
ACTGCCACAA GGGAATTCCA GACATATTCG GGGCTGCACT TCACTGTAAC TGCTTACAGT 
GGCTGGATTA AAGGAGTTCC GCGAAATGGA TTCAGAGTGG CAGTGTCGAG GGGCATCATC 
CTCACATTAC TGGATGATGT TACAAGTTGG TTGCCTGGTA ACAGAATAGT CGTTGCTAGT 
ACCGATTACT CAATGCATCA GACTGAAGAA TTCACCCTGC TTCCCTGCCC TGAATGCAGA 
AGTAATCAAG TCAAAATTAG CGGTAGCCCA CTTTATTTGC ATATTGGTGA AGTTATTGAT 
GGAATTGACA TGAGAGCAGA GGTTGGGGTT CTCACAAGAA ATGTTCTTAT CCATGGTGAA 
ATGGAAGATT TATGTTATGG ACAAAACCAG TGTCAGTTTT TCAACTATGA TACCTTTGGA 
GGACAAGTCA AGATTCAAGG AAGATTTGCA TCAGTTCATC TGTCAGGAGT AGAACTAAAA 
AACATGGGGC AGCAAATCTT GGGCAGTTAC CCAGTTCATT TCCACATGAC AGGAACTGTA 
GATGAGGAAG GGGGCTATAA CCCTCCAACG TATGTGGACA ATCTTTCCAT CCACCACTGT 
TTCTCGAGAT GTGTGGTGAT TCATGGGACC CATGGACTTC TGGTGAAAGA CACGATTGGC 
TATGACACAC TGGGGCACTG TTTTTTTCTT GAGGATGGTG TTGAACAAAG AAATACATTT 
TATCACAATC TAGGATTGCT GACAAAGGCT GGGACCATCT TGCCCACAGA CCGAAGTGAA 
GCCATGTGTC TTGCTATCCG AGATTCTGTC TATGGAGATT ATGTTCCTGT GCCTAGCATT 
GACTGCATGG CTGTCAGCAC ATTTTGGATT GCAAACCCTA ATAACAATTT AATAGGAAAT 
GCTGCAGGAG GGGCACAGGA TGTTGGCATA TGGTTCATAT TCCACCGAGT TCCCTCTGGC 
CTTTGTGAAG GACAGTATGC TGAAGGACAT TCAGAATTTA CCCCATTGGG GGTGTTTTAC 
AACAACAGGG TACACTCCAA CTTCAAGGCC GGATTATTCA TTGGCAAAGG AGTCAAAACC 
ACAGTAGCAA ATGCTGAGAA TCCAAGGGAA TATCTGACAG TAGATAATGC CCGGTTTCGT 
CCCCATCAAG ATTCAGATCC CACCAAGCCT CGAATACCAG CTGTTATTGA TGGCCTAATA 
GCTTTCAAAA ACAATGACCA TGGTGCATGG GCTAGAGGAG GGGACATTAT TTTCCGTAAC 
TCAGGGTTTT CTGATAATGG CATTGGACTA ACTTTAGCCA GTGATGGGAC ATTTCCTACT 
GATGAAGGCT CTAGCTTGGA GATCACAGAC TCCATATTTG TGGGTGAGAG CGAGAATGTG 
GGATCACAAG GTGGACAGAA CATCTACTGG GGACGTGGGG GTTTGGGGGA GCAGCGATCA 
TTTCCAAGAA ACAAGACATT CCCAGTTCGT GGTTTCCAAA TATACGATGG GCCCGTCAGG 
CTCCTGAAGT GCACTTTCAA GAAATTTCTC CCAACTCTTG ACAGACCTGT GAGCGCTATT 
GGGTTCTCCT TGAAAAATTC TTGGCAGATA ACTCCACAGA ACAATGTGTC CCTGGTCAGG 
ATGGAATGGA ATGTTGGGCT GAAGGTTTTC TTTGGTCATT CAGGACAATG GTTTGGCTCA 
AATAACTTTG ATGGTGATAA AGTGTCAATC TTCCATGATT TGGATGGCTC AGTGACTGGA 
TATCCTGATA CCTTTATAGG AAGGGCAGAT AATTACCTGA TCCGTCACCC TGGATGCCTC 
ACTGTGCCAC GGTGGAACGG AGTGATTTGT TCAGGCAGGT ATGCCCAGCT TTACATCCAG 
GCCAGACGCC CTGAGAATCT GAACATTTTG GTCTCTAGAG CAACTTATCC CTCTGTTCCT 
TTAAAACTTT CTGGCGTTAA CCGGGGTGCA CCATATCAAC AGTACCAACC AGTGGTCATG 
CTGCATCAAT CCTATATTAT CAGATGGGAA GGTCAGTCTC CTGGAGAGGT CATCCTATAC 
CCCATCAACT TTAACAGGGG AGACTGGATT CAGATTGCCT TGTGTTATCC CAGAAGTGCA 
CAGTTCCAGG TCATGTCAGA TGTTTATGAT CGTCACAGTG GAAGGATACA CAGCGTAAGA 
CACTATTCCT GGGCTCATAC CATCAGCCAG GTTCTTAATC AGTCAGAGAC AAGACTTTTT 
CATTTCAGTC AGGAATCTGG GTACTTGTTC ATACACCTAC AGGCACACTC AGTCCGCAGT 
GGGCACAGCT ATTGTTCCCA GCATGGCTGT GAGCGAATTA AGATAGTGTC TCAATTTGAA 
CTAGAGGATT CCAGTGAATG TATTCAGAAC CCATTTCCTA AAACAGATAG TTATACCCAT 
ACCCGCCGAC TCTTTGCATC TCACCGACTT GCCCTGCCAT GCAGTGAATG TGGTGCTCCA 
CAGGTAGCAA TAACAAGCAA CCCATCTATG AAATATACAA GAATACAGAT CCAATCCCTC 
CGTATGGCAG ATAATGTAAA TGGACTGAAA GCTGCATTTA TCAAGGTTGA TGAAGACATT 
TTTTTGATCA AGAGAAAGGG GCTTTTCTTC CTGGTGTTAG ATGCCTGCTC TGGAGCTGTT 
GTAAGCAAGA GGCAATTTGA AATGATCAGT GATTTGAAGA TAACAAAAGC AATTGCCAGT 
TACATTCAGA CCTCAATGAA ACTAAGATCA GTCGTTCTTA TTTGCTCCAG GGACATTGTG 
GAGACTCCGC CTAGTTCTGA AATGTCTGTT TTTACTAAAA TTGGCTCAGC CAAACCCATT 
GTACTTCCAA AGAAAGGTAG TTTTGCTATG GTAGGATTTA AAGGGCTTTC CACTCCATCC 
TGGATTAAAA TCATTAATAA CAGTGCAGAT AGAAAAGCTG CATCGATTCA CCTTTATCTT 
CCACTGATGT TGACTGAATA TAGATGCCCG GCCCCAAAAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100491930 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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