plch2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	plch2
Entrez Gene ID	100487353
Full Name	phospholipase C eta 2
General protein information	Preferred Names phospholipase C eta 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096046.1	XP_017951535.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_018096047.1	XP_017951536.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_031905490.1	XP_031761350.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_031905493.1	XP_031761353.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
XM_018096045.1	XP_017951534.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_031905491.1	XP_031761351.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_031905492.1	XP_031761352.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_018096045.2	XP_017951534.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLCH2	NP_055453.2
Pan troglodytes (chimpanzee)	PLCH2	XP_003307816.2
Mus musculus (house mouse)	Plch2	NP_001106831.1
Oryza sativa (rice)	Os03g0289300	NP_001049792.2
	PLCH2	XP_001085424.2
Arabidopsis thaliana (thale cress)	AT2G40116	NP_850327.2
Xenopus tropicalis (tropical clawed frog)	LOC100487353	XP_002936604.2
Canis lupus familiaris (dog)	PLCH2	XP_005620480.1
Bos taurus (cattle)	PLCH2	XP_005217252.1
Rattus norvegicus (Norway rat)	Plch2	XP_006239682.1
Danio rerio (zebrafish)	plch2	XP_689411.6
Gallus gallus (chicken)	PLCH2	XP_425738.3
Oryza sativa (rice)	Os07g0694000	NP_001060735.1

Gene Ontology

The following plch2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plch2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45898	XM_018096047.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa61848	XM_031905493.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa45896	XM_018096045.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa45897	XM_018096046.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa61844	XM_031905490.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa61845	XM_031905491.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa61846	XM_018096045.2 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa61847	XM_031905492.1 Latest version!	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plch2 ( XM_018096047.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951536.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa45898
Clone ID Related Accession (Same CDS sequence)	XM_018096047.1
Accession Version	XM_018096047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT 
GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC 
ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC 
TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA 
GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG 
AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC 
CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA 
ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC 
CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA 
GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC 
TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT 
TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG 
TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC 
ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA 
CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG 
GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG 
GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG 
GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG 
GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG 
ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT 
GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT 
CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA 
CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA 
AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA 
CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA 
AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT 
TTAAAAGTTA AAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951536.1
CDS	89..3106
Translation	MVRYSWHFWRLKKRLNYQVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDWKASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKVKD

Target ORF information:

RefSeq Version	XM_018096047.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096047.1

ORF Insert Sequence:

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT 
GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC 
ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC 
TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA 
GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG 
AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC 
CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA 
ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC 
CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA 
GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC 
TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT 
TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG 
TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC 
ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA 
CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG 
GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG 
GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG 
GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG 
GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG 
ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT 
GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT 
CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA 
CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA 
AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA 
CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA 
AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT 
TTAAAAGTTA AAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_031905493.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761353.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa61848
Clone ID Related Accession (Same CDS sequence)	XM_031905493.1
Accession Version	XM_031905493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC 
ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG 
GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG 
AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT 
GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC 
TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA 
TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG 
CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA 
GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC 
CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG 
CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC 
CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA 
TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT 
GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC 
ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT 
CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC 
ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC 
GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT 
CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA 
TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG 
GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC 
TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA 
ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT 
GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT 
TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG 
CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA 
CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA 
TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG 
AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA 
GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG 
GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG 
AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT 
GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC 
CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC 
ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT 
GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC 
AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT 
CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG 
GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT 
CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC 
TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC 
CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT 
GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA 
GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA 
ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA 
AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC 
ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC 
AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT 
GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT 
TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA 
GTTAAAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761353.1
CDS	525..3596
Translation	MALSSPKVDIKKKPLETSVNGKEQDFVFLSENVVLVVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDWKASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKVKD

Target ORF information:

RefSeq Version	XM_031905493.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905493.1

ORF Insert Sequence:

ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC 
ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG 
GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG 
AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT 
GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC 
TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA 
TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG 
CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA 
GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC 
CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG 
CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC 
CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA 
TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT 
GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC 
ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT 
CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC 
ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC 
GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT 
CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA 
TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG 
GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC 
TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA 
ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT 
GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT 
TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG 
CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA 
CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA 
TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG 
AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA 
GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG 
GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG 
AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT 
GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC 
CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC 
ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT 
GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC 
AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT 
CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG 
GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT 
CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC 
TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC 
CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT 
GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA 
GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA 
ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA 
AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC 
ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC 
AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT 
GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT 
TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA 
GTTAAAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_018096045.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951534.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

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CloneID	OXa45896
Clone ID Related Accession (Same CDS sequence)	XM_018096045.1
Accession Version	XM_018096045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT 
GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC 
ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC 
TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA 
GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG 
AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC 
CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA 
ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC 
CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA 
GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC 
TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT 
TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG 
TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC 
ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA 
CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG 
GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG 
GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG 
GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG 
GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG 
ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT 
GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT 
CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA 
CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA 
AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA 
CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA 
AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT 
TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA 
ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT 
GCCTGTTTTA TAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA 
TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG 
CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT 
AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA 
AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT 
GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT 
TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT 
CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA 
CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA 
AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT 
ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG 
AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC 
AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT 
GACATCTCTG ACGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC 
GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT 
TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT 
ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT 
TTAAAACAGA CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG 
TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC 
AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT 
ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC 
TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT 
GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT 
GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT 
GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC 
TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951534.1
CDS	89..4771
Translation	MVRYSWHFWRLKKRLNYQVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDWKASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKDPRQLSIYRSASSLCSLETIKEEPLFAKEKPSTSPQPVACFISCESTACDQYKESQSECSSPAITSENVQRPSKLNVEPAIIKVLAISNGLHPHNGKLNPGKAYNDRNGKVLSSVSGKSDCTNLINSSLIKPGLSLLADVSRNVNEQYQVKANQAAQSSIHASNTSVANRIMCTNLPDHQTSSKPNMKHLIVPPVQRSQYDRPDSKPSVVHAGKHTPVRRAKSEGYVVSDCSALSLNSTCQSPVAEVYSDATVNDRIWSKLELNSHRDSMSSSSSKSSNDTVIDLSLPNLARKSLLDLDISDDNFENIHLNNTIRPRSATSSTKEMSLGVSKSKSNPNLVSNNQAIDELSSRPLARSPTEALPHLPRRNTWNRLYVDSLKQTNKKPSFENKPPTNQSMSKSLGDLTSEDISCNFESKYRSISRSFITRSMRAQRRPASMKGSEKSSDSLTEQLKKLTSFQKENDITSPTDFEPVHCNNDDTAFLRRTSSRSQSRVRYIANRAKQAQERQRLQNLCRINGNSIEERGNPEGACAITKGICMDSLSPTQFTSETLDKFGP

Target ORF information:

RefSeq Version	XM_018096045.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096045.1

ORF Insert Sequence:

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT 
GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC 
ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC 
TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA 
GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG 
AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC 
CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA 
ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC 
CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA 
GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC 
TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT 
TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG 
TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC 
ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA 
CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG 
GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG 
GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG 
GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG 
GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG 
ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT 
GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT 
CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA 
CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA 
AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA 
CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA 
AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT 
TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA 
ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT 
GCCTGTTTTA TAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA 
TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG 
CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT 
AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA 
AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT 
GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT 
TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT 
CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA 
CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA 
AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT 
ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG 
AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC 
AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT 
GACATCTCTG ACGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC 
GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT 
TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT 
ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT 
TTAAAACAGA CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG 
TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC 
AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT 
ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC 
TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT 
GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT 
GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT 
GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC 
TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_018096046.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951535.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa45897
Clone ID Related Accession (Same CDS sequence)	XM_018096046.1
Accession Version	XM_018096046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG 
CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA 
CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA 
TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG 
AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA 
GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG 
GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG 
AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT 
GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC 
CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC 
ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT 
GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC 
AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT 
CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG 
GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT 
CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC 
TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC 
CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT 
GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA 
GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA 
ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA 
AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC 
ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC 
AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT 
GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT 
TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA 
GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT 
AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT 
TTTATAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT 
AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC 
ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA 
GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT 
TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT 
TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT 
CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA 
ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT 
GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA 
GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT 
CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA 
CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC 
ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC 
TCTGACGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA 
TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA 
TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA 
GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA 
CAGACAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA 
AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT 
ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG 
GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG 
AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC 
ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC 
CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT 
TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG 
GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951535.1
CDS	89..4765
Translation	MVRYSWHFWRLKKRLNYQVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKDPRQLSIYRSASSLCSLETIKEEPLFAKEKPSTSPQPVACFISCESTACDQYKESQSECSSPAITSENVQRPSKLNVEPAIIKVLAISNGLHPHNGKLNPGKAYNDRNGKVLSSVSGKSDCTNLINSSLIKPGLSLLADVSRNVNEQYQVKANQAAQSSIHASNTSVANRIMCTNLPDHQTSSKPNMKHLIVPPVQRSQYDRPDSKPSVVHAGKHTPVRRAKSEGYVVSDCSALSLNSTCQSPVAEVYSDATVNDRIWSKLELNSHRDSMSSSSSKSSNDTVIDLSLPNLARKSLLDLDISDDNFENIHLNNTIRPRSATSSTKEMSLGVSKSKSNPNLVSNNQAIDELSSRPLARSPTEALPHLPRRNTWNRLYVDSLKQTNKKPSFENKPPTNQSMSKSLGDLTSEDISCNFESKYRSISRSFITRSMRAQRRPASMKGSEKSSDSLTEQLKKLTSFQKENDITSPTDFEPVHCNNDDTAFLRRTSSRSQSRVRYIANRAKQAQERQRLQNLCRINGNSIEERGNPEGACAITKGICMDSLSPTQFTSETLDKFGP

Target ORF information:

RefSeq Version	XM_018096046.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096046.1

ORF Insert Sequence:

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG 
CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA 
CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA 
TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG 
AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA 
GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG 
GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG 
AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT 
GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC 
CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC 
ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT 
GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC 
AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT 
CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG 
GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT 
CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC 
TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC 
CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT 
GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA 
GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA 
ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA 
AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC 
ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC 
AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT 
GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT 
TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA 
GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT 
AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT 
TTTATAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT 
AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC 
ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA 
GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT 
TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT 
TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT 
CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA 
ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT 
GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA 
GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT 
CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA 
CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC 
ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC 
TCTGACGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA 
TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA 
TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA 
GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA 
CAGACAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA 
AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT 
ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG 
GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG 
AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC 
ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC 
CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT 
TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG 
GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_031905490.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761350.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61844
Clone ID Related Accession (Same CDS sequence)	XM_031905490.1
Accession Version	XM_031905490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC 
ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG 
GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG 
AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT 
GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC 
TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA 
TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG 
CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA 
GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC 
CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG 
CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC 
CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA 
TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT 
GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC 
ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT 
CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC 
ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC 
GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT 
CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA 
TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG 
GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC 
TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA 
ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT 
GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT 
TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG 
CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA 
CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA 
TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG 
AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA 
GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG 
GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG 
AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT 
GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC 
CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC 
ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT 
GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC 
AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT 
CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG 
GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT 
CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC 
TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC 
CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT 
GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA 
GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA 
ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA 
AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC 
ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC 
AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT 
GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT 
TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA 
GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT 
AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT 
TTTACAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT 
AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC 
ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA 
GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT 
TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT 
TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT 
CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA 
ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT 
GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA 
GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT 
CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA 
CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC 
ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC 
TCTGATGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA 
TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA 
TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA 
GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA 
CAGGCAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA 
AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT 
ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG 
GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG 
AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC 
ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC 
CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT 
TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG 
GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761350.1
CDS	524..5260
Translation	MALSSPKVDIKKKPLETSVNGKEQDFVFLSENVVLVVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDWKASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKDPRQLSIYRSASSLCSLETIKEEPLFAKEKPSTSPQPVACFTSCESTACDQYKESQSECSSPAITSENVQRPSKLNVEPAIIKVLAISNGLHPHNGKLNPGKAYNDRNGKVLSSVSGKSDCTNLINSSLIKPGLSLLADVSRNVNEQYQVKANQAAQSSIHASNTSVANRIMCTNLPDHQTSSKPNMKHLIVPPVQRSQYDRPDSKPSVVHAGKHTPVRRAKSEGYVVSDCSALSLNSTCQSPVAEVYSDATVNDRIWSKLELNSHRDSMSSSSSKSSNDTVIDLSLPNLARKSLLDLDISDDNFENIHLNNTIRPRSATSSTKEMSLGVSKSKSNPNLVSNNQAIDELSSRPLARSPTEALPHLPRRNTWNRLYVDSLKQANKKPSFENKPPTNQSMSKSLGDLTSEDISCNFESKYRSISRSFITRSMRAQRRPASMKGSEKSSDSLTEQLKKLTSFQKENDITSPTDFEPVHCNNDDTAFLRRTSSRSQSRVRYIANRAKQAQERQRLQNLCRINGNSIEERGNPEGACAITKGICMDSLSPTQFTSETLDKFGP

Target ORF information:

RefSeq Version	XM_031905490.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905490.1

ORF Insert Sequence:

ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC 
ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG 
GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG 
AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT 
GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC 
TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA 
TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG 
CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA 
GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC 
CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG 
CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC 
CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA 
TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT 
GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC 
ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT 
CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC 
ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC 
GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT 
CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA 
TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG 
GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC 
TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA 
ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT 
GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT 
TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG 
CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA 
CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA 
TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG 
AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA 
GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG 
GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG 
AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT 
GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC 
CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC 
ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT 
GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC 
AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT 
CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG 
GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT 
CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC 
TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC 
CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT 
GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA 
GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA 
ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA 
AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC 
ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC 
AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT 
GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT 
TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA 
GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT 
AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT 
TTTACAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT 
AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC 
ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA 
GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT 
TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT 
TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT 
CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA 
ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT 
GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA 
GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT 
CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA 
CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC 
ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC 
TCTGATGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA 
TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA 
TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA 
GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA 
CAGGCAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA 
AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT 
ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG 
GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG 
AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC 
ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC 
CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT 
TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG 
GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_031905491.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761351.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

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CloneID	OXa61845
Clone ID Related Accession (Same CDS sequence)	XM_031905491.1
Accession Version	XM_031905491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC 
ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG 
GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG 
AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT 
GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC 
TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA 
TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG 
CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA 
GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC 
CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG 
CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC 
CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA 
TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT 
GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC 
ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT 
CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC 
ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC 
GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT 
CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA 
TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG 
GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC 
TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA 
ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT 
GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT 
GCATCTGCTA ACAGAAAGCA AGTGGAAAAC ATTGCAAAGA AGAAACTTGA CTCGCTGTTG 
AAAGAATCAA AAATCCGGGA CCGCGAAGAT CCTAACAATT TCACAGTCAC CACACTGCCA 
CCATCTGGGA AACTCAGCCA GAAACCAGAA TTAAAAAAGA AATGCAGCTC TGGATCTAAA 
GGGGAAAGGG CTTTGAGTAA AACAGAGGAA GAAGCTGAGT CTGGAGAAGA GCCGAGCCCC 
AACAAAAGAC ACAGTCGTTC TTTGATAGGA AGTTTTTCCA AGCACAAGAA AAAAGGTGGA 
AAAATAAAAA AATGCTCAAG CTTAGAAGAA GGGGACGAAA GCCTGGACCG CCAGGGAAGT 
TTAGCAAGAG CTTCAGTACA TTATAGCAGA ATAAATAGAC AAAAGAAAAC CATGAAGCTG 
TCAAGAGCCC TGTCTGACCT GGTAAAATAC ACCAAATCAG TTGGTATCCA TGATGTGGAA 
ACTGAAATCT CTTGCAGCTG GCAAGTTTCA TCATTTAGTG AAACAAAAGC CCACCAGATC 
CTTCAGCAAA AACCAGCTCA GTTTGTCCGG TTTAACCAAC ACCAGCTCTC TCGCATTTAT 
CCATCGTCTT ACAGAGTCGA CTCCAGCAAC TACAACCCAC AACCATTTTG GAATGCTGGT 
TGTCAATTAG TTGCTTTAAA CTACCAGTCT GAAGGAAGAA TGTTGCAGTT AAACAGAGCC 
AAGTTCAACA GCAATGGAAA CTGCGGTTAT GTGCTGAAAC CGAAATGCAT GTGTCAAGGT 
GCCTTTAATC CTAATTCTGA AGATCCTTTG CCAGGACAAT TGAAGAAACA GCTGGTTTTG 
CGAATAATCA GTGGCCAACA GCTTCCAAAG CCAAAAGACT CCATGCTGGG AGATCGTGGA 
GAGATCATTG ATCCTTTTGT AGAAGTTGAG CTTATTGGTT TGCCAGTGGA TTGCTTTAAA 
GAACAAACAC GAGTTGTTGA TGATAATGGA TTCAATCCTA TGTGGGAGGA AACCCTTGTA 
TTTACAGTGC ACATGCCAGA AATATCATTA ATTCGCTTCC TGGTGTGGGA TCATGATCCG 
ATTGGCCGGG ATTTCATTGG ACAACGGACT CTTTCATTTA GCAGCATGAT GCCAGGCTAT 
CGACATGTGT ACCTGGAAGG TATGGAGGAG GCATCCATCT TTGTTCATGT AGCAATCAAT 
GACATCAGTG GTAAGGTCAA ACAGGCCGCG GGCTTAAAAG GACTCTTTCT GAGAAATCCA 
AAGCAAGCTT CTCTGGACAG TCATGCTGCT GGACAGCTCC ATCGGAAACA TTCCATTAGT 
AGTCACATTT TAAGGAGAAC TGCAAGTGCT CCTACAAAGG GCCAACAAAA AAACAAAAAG 
GGCTTCCCAG AAATTGCCAT TGACTCCAAA GAAAACAGCT CGGATGGACC TAGTGAAGAC 
AGGGAGACTG ATGATTCGTC TCTGCCCCGC TTTGAAAAAG ATCCTGAAAG TGCTTCTCCA 
GCTCTTCCTT CTAGGGATGG AACTAATGTG GAATTAAACA GCAAGGGTTT AAAAGACCCT 
CGACAATTAT CAATTTATAG ATCGGCCTCC TCTTTATGCA GTCTTGAAAC CATTAAAGAA 
GAGCCTCTCT TTGCCAAGGA AAAACCTTCC ACTAGTCCCC AGCCTGTTGC CTGTTTTACA 
AGTTGTGAAA GCACAGCATG TGATCAGTAC AAGGAATCTC AGTCTGAATG CAGTAGCCCA 
GCTATTACCT CTGAGAATGT ACAACGACCA AGTAAACTGA ATGTTGAGCC AGCCATAATC 
AAGGTGCTTG CTATAAGTAA TGGACTACAT CCACATAATG GAAAACTTAA TCCAGGAAAA 
GCATACAATG ACAGGAATGG AAAAGTACTC AGTTCTGTCA GTGGGAAAAG TGATTGCACA 
AATCTAATTA ATTCATCCCT GATTAAGCCT GGGTTGTCTT TACTTGCTGA TGTTTCAAGA 
AATGTCAATG AACAATATCA AGTTAAAGCT AACCAAGCTG CACAATCTTC CATTCATGCT 
AGTAATACTT CAGTTGCAAA CAGAATAATG TGCACTAATC TACCTGATCA TCAAACTAGT 
TCAAAACCAA ACATGAAACA TCTGATTGTT CCTCCTGTTC AAAGATCACA GTATGACAGG 
CCTGACTCAA AACCTTCTGT GGTTCATGCT GGCAAACACA CACCTGTAAG GAGAGCAAAG 
AGTGAGGGCT ATGTTGTTTC AGACTGCTCA GCCTTAAGCT TAAATTCTAC ATGTCAGTCC 
CCTGTCGCTG AAGTATATTC TGATGCTACA GTTAATGATA GAATTTGGAG CAAACTGGAG 
TTAAACTCTC ATAGGGACAG TATGTCTTCG TCTTCCAGCA AGTCATCCAA TGACACAGTC 
ATTGATCTGT CTTTGCCTAA TTTAGCCCGG AAAAGCTTAT TGGATCTTGA CATCTCTGAT 
GATAACTTTG AAAATATACA TTTAAACAAT ACTATTCGGC CAAGATCCGC TACATCTTCA 
ACAAAGGAAA TGTCGCTAGG AGTTTCAAAA AGTAAGTCTA ACCCTAATTT AGTATCCAAT 
AACCAAGCAA TAGATGAACT CTCTTCTAGA CCACTGGCTA GAAGTCCTAC AGAAGCATTG 
CCACACTTGC CACGAAGGAA CACCTGGAAT AGATTATACG TAGATAGTTT AAAACAGGCA 
AATAAAAAGC CCAGTTTTGA GAATAAACCA CCAACTAACC AGTCAATGTC GAAAAGTCTT 
GGAGATCTGA CTTCAGAAGA TATTTCATGT AATTTTGAAA GTAAGTACAG AAGTATAAGC 
AGAAGTTTTA TTACTAGATC AATGAGGGCA CAAAGAAGAC CGGCCAGTAT GAAGGGTTCA 
GAGAAGTCTT CCGATTCACT GACCGAACAG CTGAAAAAGC TAACAAGCTT TCAGAAAGAA 
AATGATATTA CATCCCCAAC TGATTTTGAA CCTGTTCATT GTAACAATGA TGACACAGCC 
TTCCTTCGAA GGACGTCCTC CAGAAGTCAG AGTAGAGTAA GATATATTGC AAACCGAGCC 
AAGCAGGCTC AAGAAAGGCA AAGGCTCCAA AACCTGTGCC GGATAAATGG AAATTCCATA 
GAAGAAAGAG GGAATCCAGA AGGGGCATGT GCAATAACTA AAGGAATCTG TATGGATTCT 
CTTTCTCCCA CACAGTTTAC ATCTGAAACA TTGGACAAAT TTGGACCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761351.1
CDS	524..5254
Translation	MALSSPKVDIKKKPLETSVNGKEQDFVFLSENVVLVVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKDPRQLSIYRSASSLCSLETIKEEPLFAKEKPSTSPQPVACFTSCESTACDQYKESQSECSSPAITSENVQRPSKLNVEPAIIKVLAISNGLHPHNGKLNPGKAYNDRNGKVLSSVSGKSDCTNLINSSLIKPGLSLLADVSRNVNEQYQVKANQAAQSSIHASNTSVANRIMCTNLPDHQTSSKPNMKHLIVPPVQRSQYDRPDSKPSVVHAGKHTPVRRAKSEGYVVSDCSALSLNSTCQSPVAEVYSDATVNDRIWSKLELNSHRDSMSSSSSKSSNDTVIDLSLPNLARKSLLDLDISDDNFENIHLNNTIRPRSATSSTKEMSLGVSKSKSNPNLVSNNQAIDELSSRPLARSPTEALPHLPRRNTWNRLYVDSLKQANKKPSFENKPPTNQSMSKSLGDLTSEDISCNFESKYRSISRSFITRSMRAQRRPASMKGSEKSSDSLTEQLKKLTSFQKENDITSPTDFEPVHCNNDDTAFLRRTSSRSQSRVRYIANRAKQAQERQRLQNLCRINGNSIEERGNPEGACAITKGICMDSLSPTQFTSETLDKFGP

Target ORF information:

RefSeq Version	XM_031905491.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905491.1

ORF Insert Sequence:

ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC 
ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG 
GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG 
AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT 
GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC 
TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA 
TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG 
CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA 
GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC 
CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG 
CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC 
CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA 
TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT 
GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC 
ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT 
CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC 
ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC 
GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT 
CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA 
TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG 
GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC 
TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA 
ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT 
GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT 
GCATCTGCTA ACAGAAAGCA AGTGGAAAAC ATTGCAAAGA AGAAACTTGA CTCGCTGTTG 
AAAGAATCAA AAATCCGGGA CCGCGAAGAT CCTAACAATT TCACAGTCAC CACACTGCCA 
CCATCTGGGA AACTCAGCCA GAAACCAGAA TTAAAAAAGA AATGCAGCTC TGGATCTAAA 
GGGGAAAGGG CTTTGAGTAA AACAGAGGAA GAAGCTGAGT CTGGAGAAGA GCCGAGCCCC 
AACAAAAGAC ACAGTCGTTC TTTGATAGGA AGTTTTTCCA AGCACAAGAA AAAAGGTGGA 
AAAATAAAAA AATGCTCAAG CTTAGAAGAA GGGGACGAAA GCCTGGACCG CCAGGGAAGT 
TTAGCAAGAG CTTCAGTACA TTATAGCAGA ATAAATAGAC AAAAGAAAAC CATGAAGCTG 
TCAAGAGCCC TGTCTGACCT GGTAAAATAC ACCAAATCAG TTGGTATCCA TGATGTGGAA 
ACTGAAATCT CTTGCAGCTG GCAAGTTTCA TCATTTAGTG AAACAAAAGC CCACCAGATC 
CTTCAGCAAA AACCAGCTCA GTTTGTCCGG TTTAACCAAC ACCAGCTCTC TCGCATTTAT 
CCATCGTCTT ACAGAGTCGA CTCCAGCAAC TACAACCCAC AACCATTTTG GAATGCTGGT 
TGTCAATTAG TTGCTTTAAA CTACCAGTCT GAAGGAAGAA TGTTGCAGTT AAACAGAGCC 
AAGTTCAACA GCAATGGAAA CTGCGGTTAT GTGCTGAAAC CGAAATGCAT GTGTCAAGGT 
GCCTTTAATC CTAATTCTGA AGATCCTTTG CCAGGACAAT TGAAGAAACA GCTGGTTTTG 
CGAATAATCA GTGGCCAACA GCTTCCAAAG CCAAAAGACT CCATGCTGGG AGATCGTGGA 
GAGATCATTG ATCCTTTTGT AGAAGTTGAG CTTATTGGTT TGCCAGTGGA TTGCTTTAAA 
GAACAAACAC GAGTTGTTGA TGATAATGGA TTCAATCCTA TGTGGGAGGA AACCCTTGTA 
TTTACAGTGC ACATGCCAGA AATATCATTA ATTCGCTTCC TGGTGTGGGA TCATGATCCG 
ATTGGCCGGG ATTTCATTGG ACAACGGACT CTTTCATTTA GCAGCATGAT GCCAGGCTAT 
CGACATGTGT ACCTGGAAGG TATGGAGGAG GCATCCATCT TTGTTCATGT AGCAATCAAT 
GACATCAGTG GTAAGGTCAA ACAGGCCGCG GGCTTAAAAG GACTCTTTCT GAGAAATCCA 
AAGCAAGCTT CTCTGGACAG TCATGCTGCT GGACAGCTCC ATCGGAAACA TTCCATTAGT 
AGTCACATTT TAAGGAGAAC TGCAAGTGCT CCTACAAAGG GCCAACAAAA AAACAAAAAG 
GGCTTCCCAG AAATTGCCAT TGACTCCAAA GAAAACAGCT CGGATGGACC TAGTGAAGAC 
AGGGAGACTG ATGATTCGTC TCTGCCCCGC TTTGAAAAAG ATCCTGAAAG TGCTTCTCCA 
GCTCTTCCTT CTAGGGATGG AACTAATGTG GAATTAAACA GCAAGGGTTT AAAAGACCCT 
CGACAATTAT CAATTTATAG ATCGGCCTCC TCTTTATGCA GTCTTGAAAC CATTAAAGAA 
GAGCCTCTCT TTGCCAAGGA AAAACCTTCC ACTAGTCCCC AGCCTGTTGC CTGTTTTACA 
AGTTGTGAAA GCACAGCATG TGATCAGTAC AAGGAATCTC AGTCTGAATG CAGTAGCCCA 
GCTATTACCT CTGAGAATGT ACAACGACCA AGTAAACTGA ATGTTGAGCC AGCCATAATC 
AAGGTGCTTG CTATAAGTAA TGGACTACAT CCACATAATG GAAAACTTAA TCCAGGAAAA 
GCATACAATG ACAGGAATGG AAAAGTACTC AGTTCTGTCA GTGGGAAAAG TGATTGCACA 
AATCTAATTA ATTCATCCCT GATTAAGCCT GGGTTGTCTT TACTTGCTGA TGTTTCAAGA 
AATGTCAATG AACAATATCA AGTTAAAGCT AACCAAGCTG CACAATCTTC CATTCATGCT 
AGTAATACTT CAGTTGCAAA CAGAATAATG TGCACTAATC TACCTGATCA TCAAACTAGT 
TCAAAACCAA ACATGAAACA TCTGATTGTT CCTCCTGTTC AAAGATCACA GTATGACAGG 
CCTGACTCAA AACCTTCTGT GGTTCATGCT GGCAAACACA CACCTGTAAG GAGAGCAAAG 
AGTGAGGGCT ATGTTGTTTC AGACTGCTCA GCCTTAAGCT TAAATTCTAC ATGTCAGTCC 
CCTGTCGCTG AAGTATATTC TGATGCTACA GTTAATGATA GAATTTGGAG CAAACTGGAG 
TTAAACTCTC ATAGGGACAG TATGTCTTCG TCTTCCAGCA AGTCATCCAA TGACACAGTC 
ATTGATCTGT CTTTGCCTAA TTTAGCCCGG AAAAGCTTAT TGGATCTTGA CATCTCTGAT 
GATAACTTTG AAAATATACA TTTAAACAAT ACTATTCGGC CAAGATCCGC TACATCTTCA 
ACAAAGGAAA TGTCGCTAGG AGTTTCAAAA AGTAAGTCTA ACCCTAATTT AGTATCCAAT 
AACCAAGCAA TAGATGAACT CTCTTCTAGA CCACTGGCTA GAAGTCCTAC AGAAGCATTG 
CCACACTTGC CACGAAGGAA CACCTGGAAT AGATTATACG TAGATAGTTT AAAACAGGCA 
AATAAAAAGC CCAGTTTTGA GAATAAACCA CCAACTAACC AGTCAATGTC GAAAAGTCTT 
GGAGATCTGA CTTCAGAAGA TATTTCATGT AATTTTGAAA GTAAGTACAG AAGTATAAGC 
AGAAGTTTTA TTACTAGATC AATGAGGGCA CAAAGAAGAC CGGCCAGTAT GAAGGGTTCA 
GAGAAGTCTT CCGATTCACT GACCGAACAG CTGAAAAAGC TAACAAGCTT TCAGAAAGAA 
AATGATATTA CATCCCCAAC TGATTTTGAA CCTGTTCATT GTAACAATGA TGACACAGCC 
TTCCTTCGAA GGACGTCCTC CAGAAGTCAG AGTAGAGTAA GATATATTGC AAACCGAGCC 
AAGCAGGCTC AAGAAAGGCA AAGGCTCCAA AACCTGTGCC GGATAAATGG AAATTCCATA 
GAAGAAAGAG GGAATCCAGA AGGGGCATGT GCAATAACTA AAGGAATCTG TATGGATTCT 
CTTTCTCCCA CACAGTTTAC ATCTGAAACA TTGGACAAAT TTGGACCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_018096045.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951534.2 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.

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CloneID	OXa61846
Clone ID Related Accession (Same CDS sequence)	XM_018096045.2
Accession Version	XM_018096045.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCGGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT 
GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC 
ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC 
TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA 
GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG 
AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC 
CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA 
ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC 
CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA 
GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC 
TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT 
TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG 
TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC 
ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA 
CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG 
GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG 
GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG 
GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG 
GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG 
ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT 
GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT 
CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA 
CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA 
AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA 
CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA 
AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT 
TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA 
ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT 
GCCTGTTTTA CAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA 
TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG 
CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT 
AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA 
AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT 
GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT 
TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT 
CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA 
CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA 
AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT 
ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG 
AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC 
AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT 
GACATCTCTG ATGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC 
GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT 
TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT 
ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT 
TTAAAACAGG CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG 
TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC 
AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT 
ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC 
TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT 
GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT 
GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT 
GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC 
TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951534.2
CDS	88..4770
Translation	MVRYSWHFWRLKKRLNYQVERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDWKASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKDPRQLSIYRSASSLCSLETIKEEPLFAKEKPSTSPQPVACFTSCESTACDQYKESQSECSSPAITSENVQRPSKLNVEPAIIKVLAISNGLHPHNGKLNPGKAYNDRNGKVLSSVSGKSDCTNLINSSLIKPGLSLLADVSRNVNEQYQVKANQAAQSSIHASNTSVANRIMCTNLPDHQTSSKPNMKHLIVPPVQRSQYDRPDSKPSVVHAGKHTPVRRAKSEGYVVSDCSALSLNSTCQSPVAEVYSDATVNDRIWSKLELNSHRDSMSSSSSKSSNDTVIDLSLPNLARKSLLDLDISDDNFENIHLNNTIRPRSATSSTKEMSLGVSKSKSNPNLVSNNQAIDELSSRPLARSPTEALPHLPRRNTWNRLYVDSLKQANKKPSFENKPPTNQSMSKSLGDLTSEDISCNFESKYRSISRSFITRSMRAQRRPASMKGSEKSSDSLTEQLKKLTSFQKENDITSPTDFEPVHCNNDDTAFLRRTSSRSQSRVRYIANRAKQAQERQRLQNLCRINGNSIEERGNPEGACAITKGICMDSLSPTQFTSETLDKFGP

Target ORF information:

RefSeq Version	XM_018096045.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096045.2

ORF Insert Sequence:

ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA 
GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA 
AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA 
CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT 
ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT 
CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT 
TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG 
AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC 
AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA 
GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC 
CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA 
AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT 
ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT 
GGTTTCACTA ACTATATGCG CAGTCCGGCG GGTGATATCT TCAATCCAGA CCACTATCAA 
GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA 
TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG 
CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA 
GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT 
AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC 
AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA 
AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG 
GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA 
GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT 
GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT 
GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC 
ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC 
TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA 
GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG 
AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC 
CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA 
ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC 
CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA 
GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC 
TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT 
TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG 
TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC 
ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA 
CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG 
GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG 
GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG 
GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG 
GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG 
ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT 
GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT 
CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA 
CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA 
AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA 
CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA 
AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT 
TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA 
ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT 
GCCTGTTTTA CAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA 
TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG 
CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT 
AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA 
AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT 
GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT 
TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT 
CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA 
CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA 
AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT 
ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG 
AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC 
AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT 
GACATCTCTG ATGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC 
GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT 
TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT 
ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT 
TTAAAACAGG CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG 
TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC 
AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT 
ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC 
TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT 
GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT 
GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT 
GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC 
TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plch2 ( XM_031905492.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761352.1 Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X4, mRNA.

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CloneID	OXa61847
Clone ID Related Accession (Same CDS sequence)	XM_031905492.1
Accession Version	XM_031905492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAG GAAGTCTTCC GCAGAAGCAC ATGGAAAGAT GCATGAGTTC TATGCAAGCT 
GGTACCCAAA TGATCAAACT CCGGGGTAGC TCCAAAGGTT TGGTGCGTTT TTATTACCTT 
GACGAGCACA AGTCCTGTAT AAGGTGGAGA CCCTCAAGAA AGAATGAAAA GGCCAAGATC 
TCCATTGATT CCATTCAGGA GGTGTGTGAG GGGAAACAGT CTGAGATTTT CCAGCGTTAT 
GCAGATGGAA GCTTTGATCC AAACTGCTGT TTCAGTATCT ACTATGGGGA TCACATGGAG 
TCTTTGGACT TGGTGTCCTC GAGTGGCGAA GAGGCTCGCA CATGGATTAC TGGCCTGAAA 
TATCTCATGG CAGGGATAAG TGACGAAGAC AGTCTTTCCA AGCGTCAAAG AACACGAGAC 
CAATGGTTGA AGCAGACATT TGACGAGGCA GACAAGAATG GAGACGGGTG CTTGAGTATT 
AATGAGGTCT TACAGCTCAT GCACAAACTG AATGTCAACC TCCCACGACA GAAAGTGAAG 
CAAATGTTCA AGGAGGCAGA CACTGACGAT AACCAAGGCA CGCTTGGTTT TGATGAGTTC 
TGTGCATTTT ATAAGATGAT GTCAACAAGA AGGGACCTAT ACCTGCTAAT GCTTACATAC 
AGCAACCACA AGGACCACTT GGATCCAGAT GACCTAAAAC GATTCCTAGA AGTTGAACAA 
AAGATGATAA ATGTGACAAA GGAATACTGC TTGGATATTG TTGATAAATT TGAACCATGC 
CCGGAGAATA AAAAGCAAGG GGCACTTGGA ATAGATGGTT TCACTAACTA TATGCGCAGT 
CCGGCGGGTG ATATCTTCAA TCCAGACCAC TATCAAGTGA ATCAGGATAT GACCTTACCC 
TTGAGCAACT ATTTCATTGC ATCCTCTCAC AACACATATC TCATGGGAGA TCAGTTAATG 
TCTCAGTCTC GAGTAGACAT GTACGCGTGG GTATTGCAAG CCGGTTGTCG TTGTGTTGAG 
GTGGACTGTT GGGACGGACC TGATGGAGAA CCAATAGTTC ATCATGGCTA TACTTTAACC 
TCTAAAATTC TTTTCAAAGA TGTGATAGAA ACAATTAACA AATATGCCTT CATCAGAAAT 
GACTACCCTG TCATCTTGTC TATTGAAAAT CACTGCAGTG TGGTACAGCA GAAGAAGATG 
GCTCAGTACC TGATTGAAAT CTTGGGCGAG AAATTAAATC TCTCTGATGT AGTCAATGAA 
GATTCAACAA AACTGCCGTC ACCGGAAAGC TTAAGGGGAA AAATATTAGT GAAGGGAAAG 
AAGCTTCCTG CCAGTATCAG CGATGACGCA GAGGAAGGAG ATGTTTCAGA TGAAGACAGT 
GCAGATGAGA TTGATGATGA CTGCAAGTTA ATAAATGGCG ATTGGAAGGC ATCTGCTAAC 
AGAAAGCAAG TGGAAAACAT TGCAAAGAAG AAACTTGACT CGCTGTTGAA AGAATCAAAA 
ATCCGGGACC GCGAAGATCC TAACAATTTC ACAGTCACCA CACTGCCACC ATCTGGGAAA 
CTCAGCCAGA AACCAGAATT AAAAAAGAAA TGCAGCTCTG GATCTAAAGG GGAAAGGGCT 
TTGAGTAAAA CAGAGGAAGA AGCTGAGTCT GGAGAAGAGC CGAGCCCCAA CAAAAGACAC 
AGTCGTTCTT TGATAGGAAG TTTTTCCAAG CACAAGAAAA AAGGTGGAAA AATAAAAAAA 
TGCTCAAGCT TAGAAGAAGG GGACGAAAGC CTGGACCGCC AGGGAAGTTT AGCAAGAGCT 
TCAGTACATT ATAGCAGAAT AAATAGACAA AAGAAAACCA TGAAGCTGTC AAGAGCCCTG 
TCTGACCTGG TAAAATACAC CAAATCAGTT GGTATCCATG ATGTGGAAAC TGAAATCTCT 
TGCAGCTGGC AAGTTTCATC ATTTAGTGAA ACAAAAGCCC ACCAGATCCT TCAGCAAAAA 
CCAGCTCAGT TTGTCCGGTT TAACCAACAC CAGCTCTCTC GCATTTATCC ATCGTCTTAC 
AGAGTCGACT CCAGCAACTA CAACCCACAA CCATTTTGGA ATGCTGGTTG TCAATTAGTT 
GCTTTAAACT ACCAGTCTGA AGGAAGAATG TTGCAGTTAA ACAGAGCCAA GTTCAACAGC 
AATGGAAACT GCGGTTATGT GCTGAAACCG AAATGCATGT GTCAAGGTGC CTTTAATCCT 
AATTCTGAAG ATCCTTTGCC AGGACAATTG AAGAAACAGC TGGTTTTGCG AATAATCAGT 
GGCCAACAGC TTCCAAAGCC AAAAGACTCC ATGCTGGGAG ATCGTGGAGA GATCATTGAT 
CCTTTTGTAG AAGTTGAGCT TATTGGTTTG CCAGTGGATT GCTTTAAAGA ACAAACACGA 
GTTGTTGATG ATAATGGATT CAATCCTATG TGGGAGGAAA CCCTTGTATT TACAGTGCAC 
ATGCCAGAAA TATCATTAAT TCGCTTCCTG GTGTGGGATC ATGATCCGAT TGGCCGGGAT 
TTCATTGGAC AACGGACTCT TTCATTTAGC AGCATGATGC CAGGCTATCG ACATGTGTAC 
CTGGAAGGTA TGGAGGAGGC ATCCATCTTT GTTCATGTAG CAATCAATGA CATCAGTGGT 
AAGGTCAAAC AGGCCGCGGG CTTAAAAGGA CTCTTTCTGA GAAATCCAAA GCAAGCTTCT 
CTGGACAGTC ATGCTGCTGG ACAGCTCCAT CGGAAACATT CCATTAGTAG TCACATTTTA 
AGGAGAACTG CAAGTGCTCC TACAAAGGGC CAACAAAAAA ACAAAAAGGG CTTCCCAGAA 
ATTGCCATTG ACTCCAAAGA AAACAGCTCG GATGGACCTA GTGAAGACAG GGAGACTGAT 
GATTCGTCTC TGCCCCGCTT TGAAAAAGAT CCTGAAAGTG CTTCTCCAGC TCTTCCTTCT 
AGGGATGGAA CTAATGTGGA ATTAAACAGC AAGGGTTTAA AAGACCCTCG ACAATTATCA 
ATTTATAGAT CGGCCTCCTC TTTATGCAGT CTTGAAACCA TTAAAGAAGA GCCTCTCTTT 
GCCAAGGAAA AACCTTCCAC TAGTCCCCAG CCTGTTGCCT GTTTTACAAG TTGTGAAAGC 
ACAGCATGTG ATCAGTACAA GGAATCTCAG TCTGAATGCA GTAGCCCAGC TATTACCTCT 
GAGAATGTAC AACGACCAAG TAAACTGAAT GTTGAGCCAG CCATAATCAA GGTGCTTGCT 
ATAAGTAATG GACTACATCC ACATAATGGA AAACTTAATC CAGGAAAAGC ATACAATGAC 
AGGAATGGAA AAGTACTCAG TTCTGTCAGT GGGAAAAGTG ATTGCACAAA TCTAATTAAT 
TCATCCCTGA TTAAGCCTGG GTTGTCTTTA CTTGCTGATG TTTCAAGAAA TGTCAATGAA 
CAATATCAAG TTAAAGCTAA CCAAGCTGCA CAATCTTCCA TTCATGCTAG TAATACTTCA 
GTTGCAAACA GAATAATGTG CACTAATCTA CCTGATCATC AAACTAGTTC AAAACCAAAC 
ATGAAACATC TGATTGTTCC TCCTGTTCAA AGATCACAGT ATGACAGGCC TGACTCAAAA 
CCTTCTGTGG TTCATGCTGG CAAACACACA CCTGTAAGGA GAGCAAAGAG TGAGGGCTAT 
GTTGTTTCAG ACTGCTCAGC CTTAAGCTTA AATTCTACAT GTCAGTCCCC TGTCGCTGAA 
GTATATTCTG ATGCTACAGT TAATGATAGA ATTTGGAGCA AACTGGAGTT AAACTCTCAT 
AGGGACAGTA TGTCTTCGTC TTCCAGCAAG TCATCCAATG ACACAGTCAT TGATCTGTCT 
TTGCCTAATT TAGCCCGGAA AAGCTTATTG GATCTTGACA TCTCTGATGA TAACTTTGAA 
AATATACATT TAAACAATAC TATTCGGCCA AGATCCGCTA CATCTTCAAC AAAGGAAATG 
TCGCTAGGAG TTTCAAAAAG TAAGTCTAAC CCTAATTTAG TATCCAATAA CCAAGCAATA 
GATGAACTCT CTTCTAGACC ACTGGCTAGA AGTCCTACAG AAGCATTGCC ACACTTGCCA 
CGAAGGAACA CCTGGAATAG ATTATACGTA GATAGTTTAA AACAGGCAAA TAAAAAGCCC 
AGTTTTGAGA ATAAACCACC AACTAACCAG TCAATGTCGA AAAGTCTTGG AGATCTGACT 
TCAGAAGATA TTTCATGTAA TTTTGAAAGT AAGTACAGAA GTATAAGCAG AAGTTTTATT 
ACTAGATCAA TGAGGGCACA AAGAAGACCG GCCAGTATGA AGGGTTCAGA GAAGTCTTCC 
GATTCACTGA CCGAACAGCT GAAAAAGCTA ACAAGCTTTC AGAAAGAAAA TGATATTACA 
TCCCCAACTG ATTTTGAACC TGTTCATTGT AACAATGATG ACACAGCCTT CCTTCGAAGG 
ACGTCCTCCA GAAGTCAGAG TAGAGTAAGA TATATTGCAA ACCGAGCCAA GCAGGCTCAA 
GAAAGGCAAA GGCTCCAAAA CCTGTGCCGG ATAAATGGAA ATTCCATAGA AGAAAGAGGG 
AATCCAGAAG GGGCATGTGC AATAACTAAA GGAATCTGTA TGGATTCTCT TTCTCCCACA 
CAGTTTACAT CTGAAACATT GGACAAATTT GGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761352.1
CDS	242..4900
Translation	MDLGSLPQKHMERCMSSMQAGTQMIKLRGSSKGLVRFYYLDEHKSCIRWRPSRKNEKAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSSSGEEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSINEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDPDDLKRFLEVEQKMINVTKEYCLDIVDKFEPCPENKKQGALGIDGFTNYMRSPAGDIFNPDHYQVNQDMTLPLSNYFIASSHNTYLMGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIRNDYPVILSIENHCSVVQQKKMAQYLIEILGEKLNLSDVVNEDSTKLPSPESLRGKILVKGKKLPASISDDAEEGDVSDEDSADEIDDDCKLINGDWKASANRKQVENIAKKKLDSLLKESKIRDREDPNNFTVTTLPPSGKLSQKPELKKKCSSGSKGERALSKTEEEAESGEEPSPNKRHSRSLIGSFSKHKKKGGKIKKCSSLEEGDESLDRQGSLARASVHYSRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISCSWQVSSFSETKAHQILQQKPAQFVRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFNSNGNCGYVLKPKCMCQGAFNPNSEDPLPGQLKKQLVLRIISGQQLPKPKDSMLGDRGEIIDPFVEVELIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTVHMPEISLIRFLVWDHDPIGRDFIGQRTLSFSSMMPGYRHVYLEGMEEASIFVHVAINDISGKVKQAAGLKGLFLRNPKQASLDSHAAGQLHRKHSISSHILRRTASAPTKGQQKNKKGFPEIAIDSKENSSDGPSEDRETDDSSLPRFEKDPESASPALPSRDGTNVELNSKGLKDPRQLSIYRSASSLCSLETIKEEPLFAKEKPSTSPQPVACFTSCESTACDQYKESQSECSSPAITSENVQRPSKLNVEPAIIKVLAISNGLHPHNGKLNPGKAYNDRNGKVLSSVSGKSDCTNLINSSLIKPGLSLLADVSRNVNEQYQVKANQAAQSSIHASNTSVANRIMCTNLPDHQTSSKPNMKHLIVPPVQRSQYDRPDSKPSVVHAGKHTPVRRAKSEGYVVSDCSALSLNSTCQSPVAEVYSDATVNDRIWSKLELNSHRDSMSSSSSKSSNDTVIDLSLPNLARKSLLDLDISDDNFENIHLNNTIRPRSATSSTKEMSLGVSKSKSNPNLVSNNQAIDELSSRPLARSPTEALPHLPRRNTWNRLYVDSLKQANKKPSFENKPPTNQSMSKSLGDLTSEDISCNFESKYRSISRSFITRSMRAQRRPASMKGSEKSSDSLTEQLKKLTSFQKENDITSPTDFEPVHCNNDDTAFLRRTSSRSQSRVRYIANRAKQAQERQRLQNLCRINGNSIEERGNPEGACAITKGICMDSLSPTQFTSETLDKFGP

Target ORF information:

RefSeq Version	XM_031905492.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905492.1

ORF Insert Sequence:

ATGGATTTAG GAAGTCTTCC GCAGAAGCAC ATGGAAAGAT GCATGAGTTC TATGCAAGCT 
GGTACCCAAA TGATCAAACT CCGGGGTAGC TCCAAAGGTT TGGTGCGTTT TTATTACCTT 
GACGAGCACA AGTCCTGTAT AAGGTGGAGA CCCTCAAGAA AGAATGAAAA GGCCAAGATC 
TCCATTGATT CCATTCAGGA GGTGTGTGAG GGGAAACAGT CTGAGATTTT CCAGCGTTAT 
GCAGATGGAA GCTTTGATCC AAACTGCTGT TTCAGTATCT ACTATGGGGA TCACATGGAG 
TCTTTGGACT TGGTGTCCTC GAGTGGCGAA GAGGCTCGCA CATGGATTAC TGGCCTGAAA 
TATCTCATGG CAGGGATAAG TGACGAAGAC AGTCTTTCCA AGCGTCAAAG AACACGAGAC 
CAATGGTTGA AGCAGACATT TGACGAGGCA GACAAGAATG GAGACGGGTG CTTGAGTATT 
AATGAGGTCT TACAGCTCAT GCACAAACTG AATGTCAACC TCCCACGACA GAAAGTGAAG 
CAAATGTTCA AGGAGGCAGA CACTGACGAT AACCAAGGCA CGCTTGGTTT TGATGAGTTC 
TGTGCATTTT ATAAGATGAT GTCAACAAGA AGGGACCTAT ACCTGCTAAT GCTTACATAC 
AGCAACCACA AGGACCACTT GGATCCAGAT GACCTAAAAC GATTCCTAGA AGTTGAACAA 
AAGATGATAA ATGTGACAAA GGAATACTGC TTGGATATTG TTGATAAATT TGAACCATGC 
CCGGAGAATA AAAAGCAAGG GGCACTTGGA ATAGATGGTT TCACTAACTA TATGCGCAGT 
CCGGCGGGTG ATATCTTCAA TCCAGACCAC TATCAAGTGA ATCAGGATAT GACCTTACCC 
TTGAGCAACT ATTTCATTGC ATCCTCTCAC AACACATATC TCATGGGAGA TCAGTTAATG 
TCTCAGTCTC GAGTAGACAT GTACGCGTGG GTATTGCAAG CCGGTTGTCG TTGTGTTGAG 
GTGGACTGTT GGGACGGACC TGATGGAGAA CCAATAGTTC ATCATGGCTA TACTTTAACC 
TCTAAAATTC TTTTCAAAGA TGTGATAGAA ACAATTAACA AATATGCCTT CATCAGAAAT 
GACTACCCTG TCATCTTGTC TATTGAAAAT CACTGCAGTG TGGTACAGCA GAAGAAGATG 
GCTCAGTACC TGATTGAAAT CTTGGGCGAG AAATTAAATC TCTCTGATGT AGTCAATGAA 
GATTCAACAA AACTGCCGTC ACCGGAAAGC TTAAGGGGAA AAATATTAGT GAAGGGAAAG 
AAGCTTCCTG CCAGTATCAG CGATGACGCA GAGGAAGGAG ATGTTTCAGA TGAAGACAGT 
GCAGATGAGA TTGATGATGA CTGCAAGTTA ATAAATGGCG ATTGGAAGGC ATCTGCTAAC 
AGAAAGCAAG TGGAAAACAT TGCAAAGAAG AAACTTGACT CGCTGTTGAA AGAATCAAAA 
ATCCGGGACC GCGAAGATCC TAACAATTTC ACAGTCACCA CACTGCCACC ATCTGGGAAA 
CTCAGCCAGA AACCAGAATT AAAAAAGAAA TGCAGCTCTG GATCTAAAGG GGAAAGGGCT 
TTGAGTAAAA CAGAGGAAGA AGCTGAGTCT GGAGAAGAGC CGAGCCCCAA CAAAAGACAC 
AGTCGTTCTT TGATAGGAAG TTTTTCCAAG CACAAGAAAA AAGGTGGAAA AATAAAAAAA 
TGCTCAAGCT TAGAAGAAGG GGACGAAAGC CTGGACCGCC AGGGAAGTTT AGCAAGAGCT 
TCAGTACATT ATAGCAGAAT AAATAGACAA AAGAAAACCA TGAAGCTGTC AAGAGCCCTG 
TCTGACCTGG TAAAATACAC CAAATCAGTT GGTATCCATG ATGTGGAAAC TGAAATCTCT 
TGCAGCTGGC AAGTTTCATC ATTTAGTGAA ACAAAAGCCC ACCAGATCCT TCAGCAAAAA 
CCAGCTCAGT TTGTCCGGTT TAACCAACAC CAGCTCTCTC GCATTTATCC ATCGTCTTAC 
AGAGTCGACT CCAGCAACTA CAACCCACAA CCATTTTGGA ATGCTGGTTG TCAATTAGTT 
GCTTTAAACT ACCAGTCTGA AGGAAGAATG TTGCAGTTAA ACAGAGCCAA GTTCAACAGC 
AATGGAAACT GCGGTTATGT GCTGAAACCG AAATGCATGT GTCAAGGTGC CTTTAATCCT 
AATTCTGAAG ATCCTTTGCC AGGACAATTG AAGAAACAGC TGGTTTTGCG AATAATCAGT 
GGCCAACAGC TTCCAAAGCC AAAAGACTCC ATGCTGGGAG ATCGTGGAGA GATCATTGAT 
CCTTTTGTAG AAGTTGAGCT TATTGGTTTG CCAGTGGATT GCTTTAAAGA ACAAACACGA 
GTTGTTGATG ATAATGGATT CAATCCTATG TGGGAGGAAA CCCTTGTATT TACAGTGCAC 
ATGCCAGAAA TATCATTAAT TCGCTTCCTG GTGTGGGATC ATGATCCGAT TGGCCGGGAT 
TTCATTGGAC AACGGACTCT TTCATTTAGC AGCATGATGC CAGGCTATCG ACATGTGTAC 
CTGGAAGGTA TGGAGGAGGC ATCCATCTTT GTTCATGTAG CAATCAATGA CATCAGTGGT 
AAGGTCAAAC AGGCCGCGGG CTTAAAAGGA CTCTTTCTGA GAAATCCAAA GCAAGCTTCT 
CTGGACAGTC ATGCTGCTGG ACAGCTCCAT CGGAAACATT CCATTAGTAG TCACATTTTA 
AGGAGAACTG CAAGTGCTCC TACAAAGGGC CAACAAAAAA ACAAAAAGGG CTTCCCAGAA 
ATTGCCATTG ACTCCAAAGA AAACAGCTCG GATGGACCTA GTGAAGACAG GGAGACTGAT 
GATTCGTCTC TGCCCCGCTT TGAAAAAGAT CCTGAAAGTG CTTCTCCAGC TCTTCCTTCT 
AGGGATGGAA CTAATGTGGA ATTAAACAGC AAGGGTTTAA AAGACCCTCG ACAATTATCA 
ATTTATAGAT CGGCCTCCTC TTTATGCAGT CTTGAAACCA TTAAAGAAGA GCCTCTCTTT 
GCCAAGGAAA AACCTTCCAC TAGTCCCCAG CCTGTTGCCT GTTTTACAAG TTGTGAAAGC 
ACAGCATGTG ATCAGTACAA GGAATCTCAG TCTGAATGCA GTAGCCCAGC TATTACCTCT 
GAGAATGTAC AACGACCAAG TAAACTGAAT GTTGAGCCAG CCATAATCAA GGTGCTTGCT 
ATAAGTAATG GACTACATCC ACATAATGGA AAACTTAATC CAGGAAAAGC ATACAATGAC 
AGGAATGGAA AAGTACTCAG TTCTGTCAGT GGGAAAAGTG ATTGCACAAA TCTAATTAAT 
TCATCCCTGA TTAAGCCTGG GTTGTCTTTA CTTGCTGATG TTTCAAGAAA TGTCAATGAA 
CAATATCAAG TTAAAGCTAA CCAAGCTGCA CAATCTTCCA TTCATGCTAG TAATACTTCA 
GTTGCAAACA GAATAATGTG CACTAATCTA CCTGATCATC AAACTAGTTC AAAACCAAAC 
ATGAAACATC TGATTGTTCC TCCTGTTCAA AGATCACAGT ATGACAGGCC TGACTCAAAA 
CCTTCTGTGG TTCATGCTGG CAAACACACA CCTGTAAGGA GAGCAAAGAG TGAGGGCTAT 
GTTGTTTCAG ACTGCTCAGC CTTAAGCTTA AATTCTACAT GTCAGTCCCC TGTCGCTGAA 
GTATATTCTG ATGCTACAGT TAATGATAGA ATTTGGAGCA AACTGGAGTT AAACTCTCAT 
AGGGACAGTA TGTCTTCGTC TTCCAGCAAG TCATCCAATG ACACAGTCAT TGATCTGTCT 
TTGCCTAATT TAGCCCGGAA AAGCTTATTG GATCTTGACA TCTCTGATGA TAACTTTGAA 
AATATACATT TAAACAATAC TATTCGGCCA AGATCCGCTA CATCTTCAAC AAAGGAAATG 
TCGCTAGGAG TTTCAAAAAG TAAGTCTAAC CCTAATTTAG TATCCAATAA CCAAGCAATA 
GATGAACTCT CTTCTAGACC ACTGGCTAGA AGTCCTACAG AAGCATTGCC ACACTTGCCA 
CGAAGGAACA CCTGGAATAG ATTATACGTA GATAGTTTAA AACAGGCAAA TAAAAAGCCC 
AGTTTTGAGA ATAAACCACC AACTAACCAG TCAATGTCGA AAAGTCTTGG AGATCTGACT 
TCAGAAGATA TTTCATGTAA TTTTGAAAGT AAGTACAGAA GTATAAGCAG AAGTTTTATT 
ACTAGATCAA TGAGGGCACA AAGAAGACCG GCCAGTATGA AGGGTTCAGA GAAGTCTTCC 
GATTCACTGA CCGAACAGCT GAAAAAGCTA ACAAGCTTTC AGAAAGAAAA TGATATTACA 
TCCCCAACTG ATTTTGAACC TGTTCATTGT AACAATGATG ACACAGCCTT CCTTCGAAGG 
ACGTCCTCCA GAAGTCAGAG TAGAGTAAGA TATATTGCAA ACCGAGCCAA GCAGGCTCAA 
GAAAGGCAAA GGCTCCAAAA CCTGTGCCGG ATAAATGGAA ATTCCATAGA AGAAAGAGGG 
AATCCAGAAG GGGCATGTGC AATAACTAAA GGAATCTGTA TGGATTCTCT TTCTCCCACA 
CAGTTTACAT CTGAAACATT GGACAAATTT GGACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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plch2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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