PLCH2 cDNA ORF clone, Bos taurus(cattle)

The following PLCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa49029 XM_015466868.1
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa49029 XM_015475186.1
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa49029 XM_010813422.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa49029 XM_010823142.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa92997 XM_015475187.1
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa92997 XM_015466869.1
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa49028 XM_010813421.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49028 XM_010823141.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa49020 XM_010823137.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49020 XM_010813417.3
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49021 XM_024976789.1
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49026 XM_010813415.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49026 XM_010823135.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49027 XM_010813416.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49027 XM_010823136.2
Latest version!
Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa49020 XM_010813417.2 Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa49027 XM_010813416.1 Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa49029
    Clone ID Related Accession (Same CDS sequence) XM_015475186.1 , XM_015466868.1 , XM_010823142.2 , XM_010813422.2
    Accession Version XM_015475186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330672.1
    CDS350..3328
    Translation

    Target ORF information:

    RefSeq Version XM_015475186.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49029
    Clone ID Related Accession (Same CDS sequence) XM_015475186.1 , XM_015466868.1 , XM_010823142.2 , XM_010813422.2
    Accession Version XM_015466868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322354.1
    CDS350..3328
    Translation

    Target ORF information:

    RefSeq Version XM_015466868.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49029
    Clone ID Related Accession (Same CDS sequence) XM_015475186.1 , XM_015466868.1 , XM_010823142.2 , XM_010813422.2
    Accession Version XM_010823142.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821444.1
    CDS350..3328
    Translation

    Target ORF information:

    RefSeq Version XM_010823142.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823142.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49029
    Clone ID Related Accession (Same CDS sequence) XM_015475186.1 , XM_015466868.1 , XM_010823142.2 , XM_010813422.2
    Accession Version XM_010813422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811724.1
    CDS350..3328
    Translation

    Target ORF information:

    RefSeq Version XM_010813422.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGTT AGGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92997
    Clone ID Related Accession (Same CDS sequence) XM_015475187.1 , XM_015466869.1
    Accession Version XM_015475187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGGTA AGGCGCCAGC GGAGAGGAGT CCAGAGCAGG AGCAGCCCCC GCGGGTGCCT
    GTGGGCCCCG GGCCTGCTGG GATGGCCGCC ACCTGCATGA AGTGTGTGGT GGGCTCCTGT
    GCTGGCGAGG ATGCTGAGGG CCTGCACAGG GGGCGGCTGC CCAGCCCGGG GCCCGAGGGT
    GGGCACTCGG CCATCAGCCA GCAGCCCCGT GCCTGGGAGG ACTCGTTGGG GGCCCCCCAC
    GCTGCCCCTG GGAGAAGCAG AGGGGCCCCC AAGGGCTCCA GGGCCCGGCA TCCCGGGCCG
    GGCGGCAGTG GCTCCATGTC CTCGGACTCC AGCAGCCCGG GCAGCCCCGA GGGGGCCACC
    CGCTGGCCCG AGGGTACCCG CAGGCAGGCA GGGGCCCTGC AGAGGGAGAT GAACGCCCTG
    TTCATTCAAA AGCTGGAGGA GATTAGAAGT AAATCCCCCG TGTTCTCCAC CGGTAAGACC
    AGACGCTCGT TGGCAGCGTC CCTGTGCTGG CCCCGCCCCA TCCCGCATGG CCTGGCCGGC
    GTCCCGCTGC GCTTCCATGT CCAGAACTGT GGCCTTCGTG TGCTCTCTGT GCTCCATCAG
    CTAGTCCTGC AGGCCCTGCT GCCCCCAGCT CCCTGTGTGC CCATGACGTG CCCCGCGGGC
    CTGACACCTC TGAGGCTGGG AAGTGACCCC AGGGTGGGGA CTGGGGAGTC TGAGCTGTGC
    CCTGCTTGCC AAGGCCTGGA GGCCCAAGCG TGGCTTCTGG AGACTAGCTC CAGTGCCAGG
    CCAAGCCTCC CTCCTTGGCC TGACTGCCAG GACCCGGAGG CAGTTTCCGG GTCTTCCTCC
    CGCCTCTCCC CAGCCCTGGC CTGCACTGCA GTCCTCTGGG AAGACTGGCC TGGGCTCCTG
    GAAGGGGCTC AGAGCCTTGA CACCAGGGCT TCAGCTTGCA AAGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330673.1
    CDS192..4019
    Translation

    Target ORF information:

    RefSeq Version XM_015475187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGGTA AGGCGCCAGC GGAGAGGAGT CCAGAGCAGG AGCAGCCCCC GCGGGTGCCT
    GTGGGCCCCG GGCCTGCTGG GATGGCCGCC ACCTGCATGA AGTGTGTGGT GGGCTCCTGT
    GCTGGCGAGG ATGCTGAGGG CCTGCACAGG GGGCGGCTGC CCAGCCCGGG GCCCGAGGGT
    GGGCACTCGG CCATCAGCCA GCAGCCCCGT GCCTGGGAGG ACTCGTTGGG GGCCCCCCAC
    GCTGCCCCTG GGAGAAGCAG AGGGGCCCCC AAGGGCTCCA GGGCCCGGCA TCCCGGGCCG
    GGCGGCAGTG GCTCCATGTC CTCGGACTCC AGCAGCCCGG GCAGCCCCGA GGGGGCCACC
    CGCTGGCCCG AGGGTACCCG CAGGCAGGCA GGGGCCCTGC AGAGGGAGAT GAACGCCCTG
    TTCATTCAAA AGCTGGAGGA GATTAGAAGT AAATCCCCCG TGTTCTCCAC CGGTAAGACC
    AGACGCTCGT TGGCAGCGTC CCTGTGCTGG CCCCGCCCCA TCCCGCATGG CCTGGCCGGC
    GTCCCGCTGC GCTTCCATGT CCAGAACTGT GGCCTTCGTG TGCTCTCTGT GCTCCATCAG
    CTAGTCCTGC AGGCCCTGCT GCCCCCAGCT CCCTGTGTGC CCATGACGTG CCCCGCGGGC
    CTGACACCTC TGAGGCTGGG AAGTGACCCC AGGGTGGGGA CTGGGGAGTC TGAGCTGTGC
    CCTGCTTGCC AAGGCCTGGA GGCCCAAGCG TGGCTTCTGG AGACTAGCTC CAGTGCCAGG
    CCAAGCCTCC CTCCTTGGCC TGACTGCCAG GACCCGGAGG CAGTTTCCGG GTCTTCCTCC
    CGCCTCTCCC CAGCCCTGGC CTGCACTGCA GTCCTCTGGG AAGACTGGCC TGGGCTCCTG
    GAAGGGGCTC AGAGCCTTGA CACCAGGGCT TCAGCTTGCA AAGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92997
    Clone ID Related Accession (Same CDS sequence) XM_015475187.1 , XM_015466869.1
    Accession Version XM_015466869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGGTA AGGCGCCAGC GGAGAGGAGT CCAGAGCAGG AGCAGCCCCC GCGGGTGCCT
    GTGGGCCCCG GGCCTGCTGG GATGGCCGCC ACCTGCATGA AGTGTGTGGT GGGCTCCTGT
    GCTGGCGAGG ATGCTGAGGG CCTGCACAGG GGGCGGCTGC CCAGCCCGGG GCCCGAGGGT
    GGGCACTCGG CCATCAGCCA GCAGCCCCGT GCCTGGGAGG ACTCGTTGGG GGCCCCCCAC
    GCTGCCCCTG GGAGAAGCAG AGGGGCCCCC AAGGGCTCCA GGGCCCGGCA TCCCGGGCCG
    GGCGGCAGTG GCTCCATGTC CTCGGACTCC AGCAGCCCGG GCAGCCCCGA GGGGGCCACC
    CGCTGGCCCG AGGGTACCCG CAGGCAGGCA GGGGCCCTGC AGAGGGAGAT GAACGCCCTG
    TTCATTCAAA AGCTGGAGGA GATTAGAAGT AAATCCCCCG TGTTCTCCAC CGGTAAGACC
    AGACGCTCGT TGGCAGCGTC CCTGTGCTGG CCCCGCCCCA TCCCGCATGG CCTGGCCGGC
    GTCCCGCTGC GCTTCCATGT CCAGAACTGT GGCCTTCGTG TGCTCTCTGT GCTCCATCAG
    CTAGTCCTGC AGGCCCTGCT GCCCCCAGCT CCCTGTGTGC CCATGACGTG CCCCGCGGGC
    CTGACACCTC TGAGGCTGGG AAGTGACCCC AGGGTGGGGA CTGGGGAGTC TGAGCTGTGC
    CCTGCTTGCC AAGGCCTGGA GGCCCAAGCG TGGCTTCTGG AGACTAGCTC CAGTGCCAGG
    CCAAGCCTCC CTCCTTGGCC TGACTGCCAG GACCCGGAGG CAGTTTCCGG GTCTTCCTCC
    CGCCTCTCCC CAGCCCTGGC CTGCACTGCA GTCCTCTGGG AAGACTGGCC TGGGCTCCTG
    GAAGGGGCTC AGAGCCTTGA CACCAGGGCT TCAGCTTGCA AAGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322355.1
    CDS192..4019
    Translation

    Target ORF information:

    RefSeq Version XM_015466869.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGGTA AGGCGCCAGC GGAGAGGAGT CCAGAGCAGG AGCAGCCCCC GCGGGTGCCT
    GTGGGCCCCG GGCCTGCTGG GATGGCCGCC ACCTGCATGA AGTGTGTGGT GGGCTCCTGT
    GCTGGCGAGG ATGCTGAGGG CCTGCACAGG GGGCGGCTGC CCAGCCCGGG GCCCGAGGGT
    GGGCACTCGG CCATCAGCCA GCAGCCCCGT GCCTGGGAGG ACTCGTTGGG GGCCCCCCAC
    GCTGCCCCTG GGAGAAGCAG AGGGGCCCCC AAGGGCTCCA GGGCCCGGCA TCCCGGGCCG
    GGCGGCAGTG GCTCCATGTC CTCGGACTCC AGCAGCCCGG GCAGCCCCGA GGGGGCCACC
    CGCTGGCCCG AGGGTACCCG CAGGCAGGCA GGGGCCCTGC AGAGGGAGAT GAACGCCCTG
    TTCATTCAAA AGCTGGAGGA GATTAGAAGT AAATCCCCCG TGTTCTCCAC CGGTAAGACC
    AGACGCTCGT TGGCAGCGTC CCTGTGCTGG CCCCGCCCCA TCCCGCATGG CCTGGCCGGC
    GTCCCGCTGC GCTTCCATGT CCAGAACTGT GGCCTTCGTG TGCTCTCTGT GCTCCATCAG
    CTAGTCCTGC AGGCCCTGCT GCCCCCAGCT CCCTGTGTGC CCATGACGTG CCCCGCGGGC
    CTGACACCTC TGAGGCTGGG AAGTGACCCC AGGGTGGGGA CTGGGGAGTC TGAGCTGTGC
    CCTGCTTGCC AAGGCCTGGA GGCCCAAGCG TGGCTTCTGG AGACTAGCTC CAGTGCCAGG
    CCAAGCCTCC CTCCTTGGCC TGACTGCCAG GACCCGGAGG CAGTTTCCGG GTCTTCCTCC
    CGCCTCTCCC CAGCCCTGGC CTGCACTGCA GTCCTCTGGG AAGACTGGCC TGGGCTCCTG
    GAAGGGGCTC AGAGCCTTGA CACCAGGGCT TCAGCTTGCA AAGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49028
    Clone ID Related Accession (Same CDS sequence) XM_010813421.2 , XM_010823141.2
    Accession Version XM_010813421.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGGT AAGGCGCCAG CGGAGAGGAG TCCAGAGCAG
    GAGCAGCCCC CGCGGGTGCC TGTGGGCCCC GGGCCTGCTG GGATGGCCGC CACCTGCATG
    AAGTGTGTGG TGGGCTCCTG TGCTGGCGAG GATGCTGAGG GCCTGCACAG GGGGCGGCTG
    CCCAGCCCGG GGCCCGAGGG TGGGCACTCG GCCATCAGCC AGCAGCCCCG TGCCTGGGAG
    GACTCGTTGG GGGCCCCCCA CGCTGCCCCT GGGAGAAGCA GAGGGGCCCC CAAGGGCTCC
    AGGGCCCGGC ATCCCGGGCC GGGCGGCAGT GGCTCCATGT CCTCGGACTC CAGCAGCCCG
    GGCAGCCCCG AGGGGGCCAC CCGCTGGCCC GAGGGTACCC GCAGGCAGGC AGGGGCCCTG
    CAGAGGGAGA TGAACGCCCT GTTCATTCAA AAGCTGGAGG AGATTAGAAG TAAATCCCCC
    GTGTTCTCCA CCGTTAGGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811723.1
    CDS1707..5150
    Translation

    Target ORF information:

    RefSeq Version XM_010813421.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813421.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGGT AAGGCGCCAG CGGAGAGGAG TCCAGAGCAG
    GAGCAGCCCC CGCGGGTGCC TGTGGGCCCC GGGCCTGCTG GGATGGCCGC CACCTGCATG
    AAGTGTGTGG TGGGCTCCTG TGCTGGCGAG GATGCTGAGG GCCTGCACAG GGGGCGGCTG
    CCCAGCCCGG GGCCCGAGGG TGGGCACTCG GCCATCAGCC AGCAGCCCCG TGCCTGGGAG
    GACTCGTTGG GGGCCCCCCA CGCTGCCCCT GGGAGAAGCA GAGGGGCCCC CAAGGGCTCC
    AGGGCCCGGC ATCCCGGGCC GGGCGGCAGT GGCTCCATGT CCTCGGACTC CAGCAGCCCG
    GGCAGCCCCG AGGGGGCCAC CCGCTGGCCC GAGGGTACCC GCAGGCAGGC AGGGGCCCTG
    CAGAGGGAGA TGAACGCCCT GTTCATTCAA AAGCTGGAGG AGATTAGAAG TAAATCCCCC
    GTGTTCTCCA CCGTTAGGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49028
    Clone ID Related Accession (Same CDS sequence) XM_010813421.2 , XM_010823141.2
    Accession Version XM_010823141.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGGT AAGGCGCCAG CGGAGAGGAG TCCAGAGCAG
    GAGCAGCCCC CGCGGGTGCC TGTGGGCCCC GGGCCTGCTG GGATGGCCGC CACCTGCATG
    AAGTGTGTGG TGGGCTCCTG TGCTGGCGAG GATGCTGAGG GCCTGCACAG GGGGCGGCTG
    CCCAGCCCGG GGCCCGAGGG TGGGCACTCG GCCATCAGCC AGCAGCCCCG TGCCTGGGAG
    GACTCGTTGG GGGCCCCCCA CGCTGCCCCT GGGAGAAGCA GAGGGGCCCC CAAGGGCTCC
    AGGGCCCGGC ATCCCGGGCC GGGCGGCAGT GGCTCCATGT CCTCGGACTC CAGCAGCCCG
    GGCAGCCCCG AGGGGGCCAC CCGCTGGCCC GAGGGTACCC GCAGGCAGGC AGGGGCCCTG
    CAGAGGGAGA TGAACGCCCT GTTCATTCAA AAGCTGGAGG AGATTAGAAG TAAATCCCCC
    GTGTTCTCCA CCGTTAGGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821443.1
    CDS1707..5150
    Translation

    Target ORF information:

    RefSeq Version XM_010823141.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823141.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGGT AAGGCGCCAG CGGAGAGGAG TCCAGAGCAG
    GAGCAGCCCC CGCGGGTGCC TGTGGGCCCC GGGCCTGCTG GGATGGCCGC CACCTGCATG
    AAGTGTGTGG TGGGCTCCTG TGCTGGCGAG GATGCTGAGG GCCTGCACAG GGGGCGGCTG
    CCCAGCCCGG GGCCCGAGGG TGGGCACTCG GCCATCAGCC AGCAGCCCCG TGCCTGGGAG
    GACTCGTTGG GGGCCCCCCA CGCTGCCCCT GGGAGAAGCA GAGGGGCCCC CAAGGGCTCC
    AGGGCCCGGC ATCCCGGGCC GGGCGGCAGT GGCTCCATGT CCTCGGACTC CAGCAGCCCG
    GGCAGCCCCG AGGGGGCCAC CCGCTGGCCC GAGGGTACCC GCAGGCAGGC AGGGGCCCTG
    CAGAGGGAGA TGAACGCCCT GTTCATTCAA AAGCTGGAGG AGATTAGAAG TAAATCCCCC
    GTGTTCTCCA CCGTTAGGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49020
    Clone ID Related Accession (Same CDS sequence) XM_010813417.2 , XM_010823137.2 , XM_010813417.3
    Accession Version XM_010813417.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGAGC CCCGCCCCTT CTCCATGCAG AGGTCGGTCT CCTCGCTGTG CAGCCTGGAA
    ACCATTGCCG AGGAGCCAGC CCTGGGTCCC GGCCCCCTAC TGCCAGGGGC AACCCCCCCC
    AGCCATGCCC CTGGAGGGCC CCAGTGCTCT ACAGGGCTCA GCACCCTCTC GGCGAGCCCC
    CCAAGGGTGG CTGTGGAAGC TCCCAGGCCC CTCCAGCTCC AGACAGGGAG CCACGCGAGC
    GCTGAGCCAG CCCCGGGAAG CAGGCAGTGG GCATGCAGCA CTGGGGGCCC TGCTGGGGCC
    TGCAAGGGGG CCCCCAGCGG CCATATAGAA GGCAAGGGCC ACCCCCGGGC TCTGGGCCAT
    GTGCCCAGAA GGGCCAAGAG CGAGGGGCAG GTGCCCTCGG AGTCTCTGGG TGGACGGTGG
    CCCCTGGCCG GGCCCTGCCC CACCGTGTCG TTGGACGCCA CTGGCGGGGA CCGGCTATGG
    CAGCGGCTGG AGCCAGGCAG CCACCGCGGC AGCGTGTCCT CGTCCTCCAG CCTGTCGTCC
    AGTGACACGG TCATCGAGCT CGCCCTGCCG GGCCTGGGCC TGGGCCTGGG CTGCGACAGC
    CTCCCAGGGA CCCTGGCAGG ACGTCTGCCC CTGCGGCCCT GCTCGGCCCC AGTCGCCTGC
    CTGGACCCAC CCGCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTGCGGGC TGCTGGCCAG
    CTGCCTGTGG GGCCGGACAG GCTGCAGCCC CTGCCCCTGG CCCCGGGGCC ACCCTGGCGC
    CGCCTCCCCC TGGCAGGCCT CCCGGACTGC ACTGTGGCTG CCAAGTCTAA GAGCCTCGGG
    GACCTGACTG CAGACGACTT TGCTCCCCGC GTGGAAAGCC TGGGCCGCGG CCTGGGCCTG
    GCTGGAGAGA GGCCGGCTGG GCGAAGGGTG CGGCCGGATG CCCTGACAGA GCAGTTGCGC
    TGGCTCACGG GGTTCCAGCA GGCTGGGGAC ATCACTTCAC CTACCAGCCT GGGCCCCACC
    GGGGAGGGGG TGGCAGGTGC CCCGGGCTTC CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC
    CGTGTGCGTG CCATCGCCAG CCGGGCCCGC CAGGCTCAGG AGCGGCAGCA GCGGCTGCGG
    GGTCCACGGG GACCCCCGGA GGAGGAGCGG GGCACCCCTG AGGGCGCCTG CTCCGTGGGC
    CAAGAGGGTT GTGGTGATGT GCAGGCCCCC TCCAAGGGCG CCACCCACCA GCCCTTAGGT
    GCTGCCGCCA CCGGCCTTCT GCTCCGATTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811719.1
    CDS187..4359
    Translation

    Target ORF information:

    RefSeq Version XM_010813417.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813417.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGAGC CCCGCCCCTT CTCCATGCAG AGGTCGGTCT CCTCGCTGTG CAGCCTGGAA
    ACCATTGCCG AGGAGCCAGC CCTGGGTCCC GGCCCCCTAC TGCCAGGGGC AACCCCCCCC
    AGCCATGCCC CTGGAGGGCC CCAGTGCTCT ACAGGGCTCA GCACCCTCTC GGCGAGCCCC
    CCAAGGGTGG CTGTGGAAGC TCCCAGGCCC CTCCAGCTCC AGACAGGGAG CCACGCGAGC
    GCTGAGCCAG CCCCGGGAAG CAGGCAGTGG GCATGCAGCA CTGGGGGCCC TGCTGGGGCC
    TGCAAGGGGG CCCCCAGCGG CCATATAGAA GGCAAGGGCC ACCCCCGGGC TCTGGGCCAT
    GTGCCCAGAA GGGCCAAGAG CGAGGGGCAG GTGCCCTCGG AGTCTCTGGG TGGACGGTGG
    CCCCTGGCCG GGCCCTGCCC CACCGTGTCG TTGGACGCCA CTGGCGGGGA CCGGCTATGG
    CAGCGGCTGG AGCCAGGCAG CCACCGCGGC AGCGTGTCCT CGTCCTCCAG CCTGTCGTCC
    AGTGACACGG TCATCGAGCT CGCCCTGCCG GGCCTGGGCC TGGGCCTGGG CTGCGACAGC
    CTCCCAGGGA CCCTGGCAGG ACGTCTGCCC CTGCGGCCCT GCTCGGCCCC AGTCGCCTGC
    CTGGACCCAC CCGCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTGCGGGC TGCTGGCCAG
    CTGCCTGTGG GGCCGGACAG GCTGCAGCCC CTGCCCCTGG CCCCGGGGCC ACCCTGGCGC
    CGCCTCCCCC TGGCAGGCCT CCCGGACTGC ACTGTGGCTG CCAAGTCTAA GAGCCTCGGG
    GACCTGACTG CAGACGACTT TGCTCCCCGC GTGGAAAGCC TGGGCCGCGG CCTGGGCCTG
    GCTGGAGAGA GGCCGGCTGG GCGAAGGGTG CGGCCGGATG CCCTGACAGA GCAGTTGCGC
    TGGCTCACGG GGTTCCAGCA GGCTGGGGAC ATCACTTCAC CTACCAGCCT GGGCCCCACC
    GGGGAGGGGG TGGCAGGTGC CCCGGGCTTC CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC
    CGTGTGCGTG CCATCGCCAG CCGGGCCCGC CAGGCTCAGG AGCGGCAGCA GCGGCTGCGG
    GGTCCACGGG GACCCCCGGA GGAGGAGCGG GGCACCCCTG AGGGCGCCTG CTCCGTGGGC
    CAAGAGGGTT GTGGTGATGT GCAGGCCCCC TCCAAGGGCG CCACCCACCA GCCCTTAGGT
    GCTGCCGCCA CCGGCCTTCT GCTCCGATTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49020
    Clone ID Related Accession (Same CDS sequence) XM_010813417.2 , XM_010823137.2 , XM_010813417.3
    Accession Version XM_010823137.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGAGC CCCGCCCCTT CTCCATGCAG AGGTCGGTCT CCTCGCTGTG CAGCCTGGAA
    ACCATTGCCG AGGAGCCAGC CCTGGGTCCC GGCCCCCTAC TGCCAGGGGC AACCCCCCCC
    AGCCATGCCC CTGGAGGGCC CCAGTGCTCT ACAGGGCTCA GCACCCTCTC GGCGAGCCCC
    CCAAGGGTGG CTGTGGAAGC TCCCAGGCCC CTCCAGCTCC AGACAGGGAG CCACGCGAGC
    GCTGAGCCAG CCCCGGGAAG CAGGCAGTGG GCATGCAGCA CTGGGGGCCC TGCTGGGGCC
    TGCAAGGGGG CCCCCAGCGG CCATATAGAA GGCAAGGGCC ACCCCCGGGC TCTGGGCCAT
    GTGCCCAGAA GGGCCAAGAG CGAGGGGCAG GTGCCCTCGG AGTCTCTGGG TGGACGGTGG
    CCCCTGGCCG GGCCCTGCCC CACCGTGTCG TTGGACGCCA CTGGCGGGGA CCGGCTATGG
    CAGCGGCTGG AGCCAGGCAG CCACCGCGGC AGCGTGTCCT CGTCCTCCAG CCTGTCGTCC
    AGTGACACGG TCATCGAGCT CGCCCTGCCG GGCCTGGGCC TGGGCCTGGG CTGCGACAGC
    CTCCCAGGGA CCCTGGCAGG ACGTCTGCCC CTGCGGCCCT GCTCGGCCCC AGTCGCCTGC
    CTGGACCCAC CCGCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTGCGGGC TGCTGGCCAG
    CTGCCTGTGG GGCCGGACAG GCTGCAGCCC CTGCCCCTGG CCCCGGGGCC ACCCTGGCGC
    CGCCTCCCCC TGGCAGGCCT CCCGGACTGC ACTGTGGCTG CCAAGTCTAA GAGCCTCGGG
    GACCTGACTG CAGACGACTT TGCTCCCCGC GTGGAAAGCC TGGGCCGCGG CCTGGGCCTG
    GCTGGAGAGA GGCCGGCTGG GCGAAGGGTG CGGCCGGATG CCCTGACAGA GCAGTTGCGC
    TGGCTCACGG GGTTCCAGCA GGCTGGGGAC ATCACTTCAC CTACCAGCCT GGGCCCCACC
    GGGGAGGGGG TGGCAGGTGC CCCGGGCTTC CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC
    CGTGTGCGTG CCATCGCCAG CCGGGCCCGC CAGGCTCAGG AGCGGCAGCA GCGGCTGCGG
    GGTCCACGGG GACCCCCGGA GGAGGAGCGG GGCACCCCTG AGGGCGCCTG CTCCGTGGGC
    CAAGAGGGTT GTGGTGATGT GCAGGCCCCC TCCAAGGGCG CCACCCACCA GCCCTTAGGT
    GCTGCCGCCA CCGGCCTTCT GCTCCGATTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821439.1
    CDS187..4359
    Translation

    Target ORF information:

    RefSeq Version XM_010823137.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823137.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGAGC CCCGCCCCTT CTCCATGCAG AGGTCGGTCT CCTCGCTGTG CAGCCTGGAA
    ACCATTGCCG AGGAGCCAGC CCTGGGTCCC GGCCCCCTAC TGCCAGGGGC AACCCCCCCC
    AGCCATGCCC CTGGAGGGCC CCAGTGCTCT ACAGGGCTCA GCACCCTCTC GGCGAGCCCC
    CCAAGGGTGG CTGTGGAAGC TCCCAGGCCC CTCCAGCTCC AGACAGGGAG CCACGCGAGC
    GCTGAGCCAG CCCCGGGAAG CAGGCAGTGG GCATGCAGCA CTGGGGGCCC TGCTGGGGCC
    TGCAAGGGGG CCCCCAGCGG CCATATAGAA GGCAAGGGCC ACCCCCGGGC TCTGGGCCAT
    GTGCCCAGAA GGGCCAAGAG CGAGGGGCAG GTGCCCTCGG AGTCTCTGGG TGGACGGTGG
    CCCCTGGCCG GGCCCTGCCC CACCGTGTCG TTGGACGCCA CTGGCGGGGA CCGGCTATGG
    CAGCGGCTGG AGCCAGGCAG CCACCGCGGC AGCGTGTCCT CGTCCTCCAG CCTGTCGTCC
    AGTGACACGG TCATCGAGCT CGCCCTGCCG GGCCTGGGCC TGGGCCTGGG CTGCGACAGC
    CTCCCAGGGA CCCTGGCAGG ACGTCTGCCC CTGCGGCCCT GCTCGGCCCC AGTCGCCTGC
    CTGGACCCAC CCGCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTGCGGGC TGCTGGCCAG
    CTGCCTGTGG GGCCGGACAG GCTGCAGCCC CTGCCCCTGG CCCCGGGGCC ACCCTGGCGC
    CGCCTCCCCC TGGCAGGCCT CCCGGACTGC ACTGTGGCTG CCAAGTCTAA GAGCCTCGGG
    GACCTGACTG CAGACGACTT TGCTCCCCGC GTGGAAAGCC TGGGCCGCGG CCTGGGCCTG
    GCTGGAGAGA GGCCGGCTGG GCGAAGGGTG CGGCCGGATG CCCTGACAGA GCAGTTGCGC
    TGGCTCACGG GGTTCCAGCA GGCTGGGGAC ATCACTTCAC CTACCAGCCT GGGCCCCACC
    GGGGAGGGGG TGGCAGGTGC CCCGGGCTTC CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC
    CGTGTGCGTG CCATCGCCAG CCGGGCCCGC CAGGCTCAGG AGCGGCAGCA GCGGCTGCGG
    GGTCCACGGG GACCCCCGGA GGAGGAGCGG GGCACCCCTG AGGGCGCCTG CTCCGTGGGC
    CAAGAGGGTT GTGGTGATGT GCAGGCCCCC TCCAAGGGCG CCACCCACCA GCCCTTAGGT
    GCTGCCGCCA CCGGCCTTCT GCTCCGATTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49020
    Clone ID Related Accession (Same CDS sequence) XM_010813417.2 , XM_010823137.2 , XM_010813417.3
    Accession Version XM_010813417.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813417.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGAGC CCCGCCCCTT CTCCATGCAG AGGTCGGTCT CCTCGCTGTG CAGCCTGGAA
    ACCATTGCCG AGGAGCCAGC CCTGGGTCCC GGCCCCCTAC TGCCAGGGGC AACCCCCCCC
    AGCCATGCCC CTGGAGGGCC CCAGTGCTCT ACAGGGCTCA GCACCCTCTC GGCGAGCCCC
    CCAAGGGTGG CTGTGGAAGC TCCCAGGCCC CTCCAGCTCC AGACAGGGAG CCACGCGAGC
    GCTGAGCCAG CCCCGGGAAG CAGGCAGTGG GCATGCAGCA CTGGGGGCCC TGCTGGGGCC
    TGCAAGGGGG CCCCCAGCGG CCATATAGAA GGCAAGGGCC ACCCCCGGGC TCTGGGCCAT
    GTGCCCAGAA GGGCCAAGAG CGAGGGGCAG GTGCCCTCGG AGTCTCTGGG TGGACGGTGG
    CCCCTGGCCG GGCCCTGCCC CACCGTGTCG TTGGACGCCA CTGGCGGGGA CCGGCTATGG
    CAGCGGCTGG AGCCAGGCAG CCACCGCGGC AGCGTGTCCT CGTCCTCCAG CCTGTCGTCC
    AGTGACACGG TCATCGAGCT CGCCCTGCCG GGCCTGGGCC TGGGCCTGGG CTGCGACAGC
    CTCCCAGGGA CCCTGGCAGG ACGTCTGCCC CTGCGGCCCT GCTCGGCCCC AGTCGCCTGC
    CTGGACCCAC CCGCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTGCGGGC TGCTGGCCAG
    CTGCCTGTGG GGCCGGACAG GCTGCAGCCC CTGCCCCTGG CCCCGGGGCC ACCCTGGCGC
    CGCCTCCCCC TGGCAGGCCT CCCGGACTGC ACTGTGGCTG CCAAGTCTAA GAGCCTCGGG
    GACCTGACTG CAGACGACTT TGCTCCCCGC GTGGAAAGCC TGGGCCGCGG CCTGGGCCTG
    GCTGGAGAGA GGCCGGCTGG GCGAAGGGTG CGGCCGGATG CCCTGACAGA GCAGTTGCGC
    TGGCTCACGG GGTTCCAGCA GGCTGGGGAC ATCACTTCAC CTACCAGCCT GGGCCCCACC
    GGGGAGGGGG TGGCAGGTGC CCCGGGCTTC CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC
    CGTGTGCGTG CCATCGCCAG CCGGGCCCGC CAGGCTCAGG AGCGGCAGCA GCGGCTGCGG
    GGTCCACGGG GACCCCCGGA GGAGGAGCGG GGCACCCCTG AGGGCGCCTG CTCCGTGGGC
    CAAGAGGGTT GTGGTGATGT GCAGGCCCCC TCCAAGGGCG CCACCCACCA GCCCTTAGGT
    GCTGCCGCCA CCGGCCTTCT GCTCCGATTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811719.1
    CDS218..4390
    Translation

    Target ORF information:

    RefSeq Version XM_010813417.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813417.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGCAC CTGGGGACCC CGGCGGGCTC AGCCTGGACC AAGTGGAGCG ATGTATGAGT 
    GCCATGCAGG CGGGGACGCA GATGGTGAAG CTCCGGGGCA GCTCCAAGGG CCTGGTCCGC
    TTCTACTTCC TGGACGAGCA TCGCTCCTGC ATCCGCTGGC GGCCCTCACG CAAGAATGAG
    AAAGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTTAGCG AGGGGCGGCA GTCGGAGATC
    TTCCAGCGCT ACCCTGATGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC
    AGCCACCGGG AGTCGCTGGA CCTGGTCTCA CCCAGCGGCG ACGAGGCGCG CACCTGGGTC
    ACGGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC CCGCCGCCAG
    CGCACCCGGG ACCAGTGGCT GAAGCAGACA TTTGACGAGG CCGACAAGAA TGGGGATGGC
    AGCCTGAGCA TTGGCGAGGT TCTGCAGCTG CTTCACAAGC TGAACGTGAA CCTGCCCCGG
    CAGAGGGTGA AGCAGATGTT CAAGGAGGCG GACACGGATG ACCACCAGGG GACACTAGGC
    TTTGAGGAGT TCTGTGCCTT CTACAAGATG ATGTCTACAC GCCGGGACCT CTACCTACTC
    ATGCTCACCT ACAGCAACCA CAAGGACCAC CTGGACGCCA CTGACCTGCT GCGCTTCCTG
    GAGGTGGAGC AGAAGATGAC GGGCGTGACC CTTGAGAGCT GTCGCGACAT CATCGAGCAG
    TTTGAACCCT GCCCAGAGAA CAAGAGTAAA GGGGCAATGG GCATTGACGG CTTCACCAAC
    TACACGCGGA GCCCCGCCGG CGACATCTTC AACCCGGAGC ACCGCCTCGT GCACCAGGAT
    ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCTC ACAACACCTA CCTCGCGGGC
    GACCAGCTCA CGTCCCAGTC GAGGGCGGAC ATGTACGCCC GGGTCCTGCA GGCCGGCTGC
    CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCTGATGGGG AGCCCATCGT GCACCATGGC
    TACACGCTGA CCTCCAAGAT CCTCTTCAAA GATGTCATCG AAACCATCAA CAAGTACGCC
    TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGCAG TGTCATCCAG
    CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATTCTTGGGG ACAAGCTGGA CCTGTCATCA
    GTGAGCAGCG AGGATGCCAC CATGCTGCCC TCTCCCCAGG TGCTCAAGGG CAAGATCCTG
    GTGAAGGGCA AGAAGCTCCC AGCCAACATC AGTGAGGATG CTGAGGAGGG CGAAGTGTCT
    GATGAGGACA GCGCAGATGA GATCGATGAG GACTGCAAGC TCCTCAATGG GGATGCCTCC
    ACCAACCGGA AACGTGTGGA AAACATTGCC AAGAGGAAAC TGGATTCCCT AATGAAGGAG
    TCCAAGATTC GGGACTGTGA AGACCCCAGC GACTTCACCG TGTCCACGCT GCCCCCACCT
    GGCAAACTTG GGCCCAAGGC AGAGGCCAAA AAGTTCGAGG AGGACGTGGA GATCGGGGAA
    GAGGCCGGAA CCAGCCGCCG GAATAGCCGG CTCCTCATGG GCAGCTTCTC CAAGCGCAAG
    AAAAAGAGCA GCAAGCTGAA GAAGGCGGCC AGTGTGGAGG AGGGGGACGA GGACCTGGAC
    TCCCAAGGCA GCCAGAGCAG GGGGGCCGCT AGGCAGAAGA AGACTATGAA GCTGTCTCGG
    GCCCTCTCAG ACCTGGTCAA GTACACCAAG TCTGTGGGCA CCCACGACGT GGAGACGGAG
    GTGGCGTCCA GCTGGCAGGT GTCCTCCTTC AGCGAGACCA AGGCTCAGCA GATCTTGCAG
    CAGAAGCCGG CGCAGTACTT ACGCTTCAAC CAGCACCAGC TCTCGCGCAT CTACCCCTCC
    TCCTACCGCG TGGACTCCAG CAACTACAAC CCGCAGCCCT TCTGGAACGC CGGCTGCCAG
    ATGGTCGCCC TGAACTACCA GTCGGAGGGG CGGATGCTGC AGCTGAACCG GGCCAAGTTC
    AGCGCCAACG GCAGCTGCGG CTACGTGCTC AAGCCTCAGT GCATGTGCCA GGGCATCTTC
    AACCCTAACT CTGAGGACCC CCTGCCCGGG CAGCTCAAGA AGCAGCTGGT GCTGCGGATC
    ATCAGTGGTC AGCAGCTGCC CAAGCCGCGG GACTCGATGC TGGGGGACCG AGGGGAGATC
    ATCGACCCCT TCGTGGAGGT GGAGGTCATC GGGCTCCCGG TGGACTGCAA CAAGGAGCAG
    ACCCGGGTGG TGGACGACAA TGGATTCAAC CCCATGTGGG AGGAGACCCT GGTGTTCACG
    GTACACATGC CTGAGATAGC GCTAGTACGC TTCCTCGTCT GGGACCACGA CCCCATTGGG
    CGTGACTTCA TCGGCCAAAG GACGCTGGCC TTCAGCAGCA TGATGCCAGG CTATCGGCAC
    GTGTACCTGG AGGGGATGGA AGAAGCCTCT ATCTTTGTCC ATGTGGCCGT CAGTGACATC
    AGTGGTAAGG TCAAGCAGGC TCTGGGCCTA AAAGGCCTGT TCCTCCGAGG CCCAAAGCCC
    GGCTCCCTGG ACAGTCATGC TGCTGGGCGG CCCCTGCCCC GGCCCTCCGT TAGCCAGCGG
    CTCCTGCGGC GCACGGCCAG CGCCCCGACC AAGAGTCAGA AGCCAGGCCG CAAGGCCTTC
    CCAGAGCTGG TCCTGGGCCT GCAGGACACC GGCTCTGAGG GGGAGGCTGG CGATGTGGCA
    CCCTCCAGCC CTGGCCCCAT CCCGGAGGCC CCAACCCGGG AGGAGCCAGG CAGCCGCAGC
    CCCCGAGAGC CCCGCCCCTT CTCCATGCAG AGGTCGGTCT CCTCGCTGTG CAGCCTGGAA
    ACCATTGCCG AGGAGCCAGC CCTGGGTCCC GGCCCCCTAC TGCCAGGGGC AACCCCCCCC
    AGCCATGCCC CTGGAGGGCC CCAGTGCTCT ACAGGGCTCA GCACCCTCTC GGCGAGCCCC
    CCAAGGGTGG CTGTGGAAGC TCCCAGGCCC CTCCAGCTCC AGACAGGGAG CCACGCGAGC
    GCTGAGCCAG CCCCGGGAAG CAGGCAGTGG GCATGCAGCA CTGGGGGCCC TGCTGGGGCC
    TGCAAGGGGG CCCCCAGCGG CCATATAGAA GGCAAGGGCC ACCCCCGGGC TCTGGGCCAT
    GTGCCCAGAA GGGCCAAGAG CGAGGGGCAG GTGCCCTCGG AGTCTCTGGG TGGACGGTGG
    CCCCTGGCCG GGCCCTGCCC CACCGTGTCG TTGGACGCCA CTGGCGGGGA CCGGCTATGG
    CAGCGGCTGG AGCCAGGCAG CCACCGCGGC AGCGTGTCCT CGTCCTCCAG CCTGTCGTCC
    AGTGACACGG TCATCGAGCT CGCCCTGCCG GGCCTGGGCC TGGGCCTGGG CTGCGACAGC
    CTCCCAGGGA CCCTGGCAGG ACGTCTGCCC CTGCGGCCCT GCTCGGCCCC AGTCGCCTGC
    CTGGACCCAC CCGCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTGCGGGC TGCTGGCCAG
    CTGCCTGTGG GGCCGGACAG GCTGCAGCCC CTGCCCCTGG CCCCGGGGCC ACCCTGGCGC
    CGCCTCCCCC TGGCAGGCCT CCCGGACTGC ACTGTGGCTG CCAAGTCTAA GAGCCTCGGG
    GACCTGACTG CAGACGACTT TGCTCCCCGC GTGGAAAGCC TGGGCCGCGG CCTGGGCCTG
    GCTGGAGAGA GGCCGGCTGG GCGAAGGGTG CGGCCGGATG CCCTGACAGA GCAGTTGCGC
    TGGCTCACGG GGTTCCAGCA GGCTGGGGAC ATCACTTCAC CTACCAGCCT GGGCCCCACC
    GGGGAGGGGG TGGCAGGTGC CCCGGGCTTC CTGCGGCGCT CCTCCTCCCG CAGCCAGAGC
    CGTGTGCGTG CCATCGCCAG CCGGGCCCGC CAGGCTCAGG AGCGGCAGCA GCGGCTGCGG
    GGTCCACGGG GACCCCCGGA GGAGGAGCGG GGCACCCCTG AGGGCGCCTG CTCCGTGGGC
    CAAGAGGGTT GTGGTGATGT GCAGGCCCCC TCCAAGGGCG CCACCCACCA GCCCTTAGGT
    GCTGCCGCCA CCGGCCTTCT GCTCCGATTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49021
    Clone ID Related Accession (Same CDS sequence) XM_024976789.1
    Accession Version XM_024976789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACGCGG GAGCCGCCCC GCGGAAGCAC ATGGAGCGAT GTATGAGTGC CATGCAGGCG 
    GGGACGCAGA TGGTGAAGCT CCGGGGCAGC TCCAAGGGCC TGGTCCGCTT CTACTTCCTG
    GACGAGCATC GCTCCTGCAT CCGCTGGCGG CCCTCACGCA AGAATGAGAA AGCCAAGATC
    TCCATCGACT CCATCCAGGA GGTTAGCGAG GGGCGGCAGT CGGAGATCTT CCAGCGCTAC
    CCTGATGGCA GCTTCGACCC CAACTGCTGC TTCAGCATCT ACCACGGCAG CCACCGGGAG
    TCGCTGGACC TGGTCTCACC CAGCGGCGAC GAGGCGCGCA CCTGGGTCAC GGGCCTGCGC
    TACCTCATGG CCGGCATCAG CGACGAGGAC AGCCTGGCCC GCCGCCAGCG CACCCGGGAC
    CAGTGGCTGA AGCAGACATT TGACGAGGCC GACAAGAATG GGGATGGCAG CCTGAGCATT
    GGCGAGGTTC TGCAGCTGCT TCACAAGCTG AACGTGAACC TGCCCCGGCA GAGGGTGAAG
    CAGATGTTCA AGGAGGCGGA CACGGATGAC CACCAGGGGA CACTAGGCTT TGAGGAGTTC
    TGTGCCTTCT ACAAGATGAT GTCTACACGC CGGGACCTCT ACCTACTCAT GCTCACCTAC
    AGCAACCACA AGGACCACCT GGACGCCACT GACCTGCTGC GCTTCCTGGA GGTGGAGCAG
    AAGATGACGG GCGTGACCCT TGAGAGCTGT CGCGACATCA TCGAGCAGTT TGAACCCTGC
    CCAGAGAACA AGAGTAAAGG GGCAATGGGC ATTGACGGCT TCACCAACTA CACGCGGAGC
    CCCGCCGGCG ACATCTTCAA CCCGGAGCAC CGCCTCGTGC ACCAGGATAT GACGCAGCCG
    CTGAGCCACT ACTTCATCAC CTCGTCTCAC AACACCTACC TCGCGGGCGA CCAGCTCACG
    TCCCAGTCGA GGGCGGACAT GTACGCCCGG GTCCTGCAGG CCGGCTGCCG CTGTGTGGAG
    GTGGACTGCT GGGACGGGCC TGATGGGGAG CCCATCGTGC ACCATGGCTA CACGCTGACC
    TCCAAGATCC TCTTCAAAGA TGTCATCGAA ACCATCAACA AGTACGCCTT CATCAAGAAT
    GAGTACCCAG TGATCCTGTC CATTGAGAAC CACTGCAGTG TCATCCAGCA GAAGAAGATG
    GCCCAGTATC TGACTGACAT TCTTGGGGAC AAGCTGGACC TGTCATCAGT GAGCAGCGAG
    GATGCCACCA TGCTGCCCTC TCCCCAGGTG CTCAAGGGCA AGATCCTGGT GAAGGGCAAG
    AAGCTCCCAG CCAACATCAG TGAGGATGCT GAGGAGGGCG AAGTGTCTGA TGAGGACAGC
    GCAGATGAGA TCGATGAGGA CTGCAAGCTC CTCAATGGGG ATGCCTCCAC CAACCGGAAA
    CGTGTGGAAA ACATTGCCAA GAGGAAACTG GATTCCCTAA TGAAGGAGTC CAAGATTCGG
    GACTGTGAAG ACCCCAGCGA CTTCACCGTG TCCACGCTGC CCCCACCTGG CAAACTTGGG
    CCCAAGGCAG AGGCCAAAAA GTTCGAGGAG GACGTGGAGA TCGGGGAAGA GGCCGGAACC
    AGCCGCCGGA ATAGCCGGCT CCTCATGGGC AGCTTCTCCA AGCGCAAGAA AAAGAGCAGC
    AAGCTGAAGA AGGCGGCCAG TGTGGAGGAG GGGGACGAGG ACCTGGACTC CCAAGGCAGC
    CAGAGCAGGG GGGCCGCTAG GCAGAAGAAG ACTATGAAGC TGTCTCGGGC CCTCTCAGAC
    CTGGTCAAGT ACACCAAGTC TGTGGGCACC CACGACGTGG AGACGGAGGT GGCGTCCAGC
    TGGCAGGTGT CCTCCTTCAG CGAGACCAAG GCTCAGCAGA TCTTGCAGCA GAAGCCGGCG
    CAGTACTTAC GCTTCAACCA GCACCAGCTC TCGCGCATCT ACCCCTCCTC CTACCGCGTG
    GACTCCAGCA ACTACAACCC GCAGCCCTTC TGGAACGCCG GCTGCCAGAT GGTCGCCCTG
    AACTACCAGT CGGAGGGGCG GATGCTGCAG CTGAACCGGG CCAAGTTCAG CGCCAACGGC
    AGCTGCGGCT ACGTGCTCAA GCCTCAGTGC ATGTGCCAGG GCATCTTCAA CCCTAACTCT
    GAGGACCCCC TGCCCGGGCA GCTCAAGAAG CAGCTGGTGC TGCGGATCAT CAGTGGTCAG
    CAGCTGCCCA AGCCGCGGGA CTCGATGCTG GGGGACCGAG GGGAGATCAT CGACCCCTTC
    GTGGAGGTGG AGGTCATCGG GCTCCCGGTG GACTGCAACA AGGAGCAGAC CCGGGTGGTG
    GACGACAATG GATTCAACCC CATGTGGGAG GAGACCCTGG TGTTCACGGT ACACATGCCT
    GAGATAGCGC TAGTACGCTT CCTCGTCTGG GACCACGACC CCATTGGGCG TGACTTCATC
    GGCCAAAGGA CGCTGGCCTT CAGCAGCATG ATGCCAGGCT ATCGGCACGT GTACCTGGAG
    GGGATGGAAG AAGCCTCTAT CTTTGTCCAT GTGGCCGTCA GTGACATCAG TGGTAAGGTC
    AAGCAGGCTC TGGGCCTAAA AGGCCTGTTC CTCCGAGGCC CAAAGCCCGG CTCCCTGGAC
    AGTCATGCTG CTGGGCGGCC CCTGCCCCGG CCCTCCGTTA GCCAGCGGCT CCTGCGGCGC
    ACGGCCAGCG CCCCGACCAA GAGTCAGAAG CCAGGCCGCA AGGCCTTCCC AGAGCTGGTC
    CTGGGCCTGC AGGACACCGG CTCTGAGGGG GAGGCTGGCG ATGTGGCACC CTCCAGCCCT
    GGCCCCATCC CGGAGGCCCC AACCCGGGAG GAGCCAGGCA GCCGCAGCCC CCGAGAGCCC
    CGCCCCTTCT CCATGCAGAG GTCGGTCTCC TCGCTGTGCA GCCTGGAAAC CATTGCCGAG
    GAGCCAGCCC TGGGTCCCGG CCCCCTACTG CCAGGGGCAA CCCCCCCCAG CCATGCCCCT
    GGAGGGCCCC AGTGCTCTAC AGGGCTCAGC ACCCTCTCGG CGAGCCCCCC AAGGGTGGCT
    GTGGAAGCTC CCAGGCCCCT CCAGCTCCAG ACAGGGAGCC ACGCGAGCGC TGAGCCAGCC
    CCGGGAAGCA GGCAGTGGGC ATGCAGCACT GGGGGCCCTG CTGGGGCCTG CAAGGGGGCC
    CCCAGCGGCC ATATAGAAGG CAAGGGCCAC CCCCGGGCTC TGGGCCATGT GCCCAGAAGG
    GCCAAGAGCG AGGGGCAGGT GCCCTCGGAG TCTCTGGGTG GACGGTGGCC CCTGGCCGGG
    CCCTGCCCCA CCGTGTCGTT GGACGCCACT GGCGGGGACC GGCTATGGCA GCGGCTGGAG
    CCAGGCAGCC ACCGCGGCAG CGTGTCCTCG TCCTCCAGCC TGTCGTCCAG TGACACGGTC
    ATCGAGCTCG CCCTGCCGGG CCTGGGCCTG GGCCTGGGCT GCGACAGCCT CCCAGGGACC
    CTGGCAGGAC GTCTGCCCCT GCGGCCCTGC TCGGCCCCAG TCGCCTGCCT GGACCCACCC
    GCTGTGACCA AGAGCAAATC CAACCCCAAC CTGCGGGCTG CTGGCCAGCT GCCTGTGGGG
    CCGGACAGGC TGCAGCCCCT GCCCCTGGCC CCGGGGCCAC CCTGGCGCCG CCTCCCCCTG
    GCAGGCCTCC CGGACTGCAC TGTGGCTGCC AAGTCTAAGA GCCTCGGGGA CCTGACTGCA
    GACGACTTTG CTCCCCGCGT GGAAAGCCTG GGCCGCGGCC TGGGCCTGGC TGGAGAGAGG
    CCGGCTGGGC GAAGGGTGCG GCCGGATGCC CTGACAGAGC AGTTGCGCTG GCTCACGGGG
    TTCCAGCAGG CTGGGGACAT CACTTCACCT ACCAGCCTGG GCCCCACCGG GGAGGGGGTG
    GCAGGTGCCC CGGGCTTCCT GCGGCGCTCC TCCTCCCGCA GCCAGAGCCG TGTGCGTGCC
    ATCGCCAGCC GGGCCCGCCA GGCTCAGGAG CGGCAGCAGC GGCTGCGGGG TCCACGGGGA
    CCCCCGGAGG AGGAGCGGGG CACCCCTGAG GGCGCCTGCT CCGTGGGCCA AGAGGGTTGT
    GGTGATGTGC AGGCCCCCTC CAAGGGCGCC ACCCACCAGC CCTTAGGTGC TGCCGCCACC
    GGCCTTCTGC TCCGATTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832557.1
    CDS85..4245
    Translation

    Target ORF information:

    RefSeq Version XM_024976789.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACGCGG GAGCCGCCCC GCGGAAGCAC ATGGAGCGAT GTATGAGTGC CATGCAGGCG 
    GGGACGCAGA TGGTGAAGCT CCGGGGCAGC TCCAAGGGCC TGGTCCGCTT CTACTTCCTG
    GACGAGCATC GCTCCTGCAT CCGCTGGCGG CCCTCACGCA AGAATGAGAA AGCCAAGATC
    TCCATCGACT CCATCCAGGA GGTTAGCGAG GGGCGGCAGT CGGAGATCTT CCAGCGCTAC
    CCTGATGGCA GCTTCGACCC CAACTGCTGC TTCAGCATCT ACCACGGCAG CCACCGGGAG
    TCGCTGGACC TGGTCTCACC CAGCGGCGAC GAGGCGCGCA CCTGGGTCAC GGGCCTGCGC
    TACCTCATGG CCGGCATCAG CGACGAGGAC AGCCTGGCCC GCCGCCAGCG CACCCGGGAC
    CAGTGGCTGA AGCAGACATT TGACGAGGCC GACAAGAATG GGGATGGCAG CCTGAGCATT
    GGCGAGGTTC TGCAGCTGCT TCACAAGCTG AACGTGAACC TGCCCCGGCA GAGGGTGAAG
    CAGATGTTCA AGGAGGCGGA CACGGATGAC CACCAGGGGA CACTAGGCTT TGAGGAGTTC
    TGTGCCTTCT ACAAGATGAT GTCTACACGC CGGGACCTCT ACCTACTCAT GCTCACCTAC
    AGCAACCACA AGGACCACCT GGACGCCACT GACCTGCTGC GCTTCCTGGA GGTGGAGCAG
    AAGATGACGG GCGTGACCCT TGAGAGCTGT CGCGACATCA TCGAGCAGTT TGAACCCTGC
    CCAGAGAACA AGAGTAAAGG GGCAATGGGC ATTGACGGCT TCACCAACTA CACGCGGAGC
    CCCGCCGGCG ACATCTTCAA CCCGGAGCAC CGCCTCGTGC ACCAGGATAT GACGCAGCCG
    CTGAGCCACT ACTTCATCAC CTCGTCTCAC AACACCTACC TCGCGGGCGA CCAGCTCACG
    TCCCAGTCGA GGGCGGACAT GTACGCCCGG GTCCTGCAGG CCGGCTGCCG CTGTGTGGAG
    GTGGACTGCT GGGACGGGCC TGATGGGGAG CCCATCGTGC ACCATGGCTA CACGCTGACC
    TCCAAGATCC TCTTCAAAGA TGTCATCGAA ACCATCAACA AGTACGCCTT CATCAAGAAT
    GAGTACCCAG TGATCCTGTC CATTGAGAAC CACTGCAGTG TCATCCAGCA GAAGAAGATG
    GCCCAGTATC TGACTGACAT TCTTGGGGAC AAGCTGGACC TGTCATCAGT GAGCAGCGAG
    GATGCCACCA TGCTGCCCTC TCCCCAGGTG CTCAAGGGCA AGATCCTGGT GAAGGGCAAG
    AAGCTCCCAG CCAACATCAG TGAGGATGCT GAGGAGGGCG AAGTGTCTGA TGAGGACAGC
    GCAGATGAGA TCGATGAGGA CTGCAAGCTC CTCAATGGGG ATGCCTCCAC CAACCGGAAA
    CGTGTGGAAA ACATTGCCAA GAGGAAACTG GATTCCCTAA TGAAGGAGTC CAAGATTCGG
    GACTGTGAAG ACCCCAGCGA CTTCACCGTG TCCACGCTGC CCCCACCTGG CAAACTTGGG
    CCCAAGGCAG AGGCCAAAAA GTTCGAGGAG GACGTGGAGA TCGGGGAAGA GGCCGGAACC
    AGCCGCCGGA ATAGCCGGCT CCTCATGGGC AGCTTCTCCA AGCGCAAGAA AAAGAGCAGC
    AAGCTGAAGA AGGCGGCCAG TGTGGAGGAG GGGGACGAGG ACCTGGACTC CCAAGGCAGC
    CAGAGCAGGG GGGCCGCTAG GCAGAAGAAG ACTATGAAGC TGTCTCGGGC CCTCTCAGAC
    CTGGTCAAGT ACACCAAGTC TGTGGGCACC CACGACGTGG AGACGGAGGT GGCGTCCAGC
    TGGCAGGTGT CCTCCTTCAG CGAGACCAAG GCTCAGCAGA TCTTGCAGCA GAAGCCGGCG
    CAGTACTTAC GCTTCAACCA GCACCAGCTC TCGCGCATCT ACCCCTCCTC CTACCGCGTG
    GACTCCAGCA ACTACAACCC GCAGCCCTTC TGGAACGCCG GCTGCCAGAT GGTCGCCCTG
    AACTACCAGT CGGAGGGGCG GATGCTGCAG CTGAACCGGG CCAAGTTCAG CGCCAACGGC
    AGCTGCGGCT ACGTGCTCAA GCCTCAGTGC ATGTGCCAGG GCATCTTCAA CCCTAACTCT
    GAGGACCCCC TGCCCGGGCA GCTCAAGAAG CAGCTGGTGC TGCGGATCAT CAGTGGTCAG
    CAGCTGCCCA AGCCGCGGGA CTCGATGCTG GGGGACCGAG GGGAGATCAT CGACCCCTTC
    GTGGAGGTGG AGGTCATCGG GCTCCCGGTG GACTGCAACA AGGAGCAGAC CCGGGTGGTG
    GACGACAATG GATTCAACCC CATGTGGGAG GAGACCCTGG TGTTCACGGT ACACATGCCT
    GAGATAGCGC TAGTACGCTT CCTCGTCTGG GACCACGACC CCATTGGGCG TGACTTCATC
    GGCCAAAGGA CGCTGGCCTT CAGCAGCATG ATGCCAGGCT ATCGGCACGT GTACCTGGAG
    GGGATGGAAG AAGCCTCTAT CTTTGTCCAT GTGGCCGTCA GTGACATCAG TGGTAAGGTC
    AAGCAGGCTC TGGGCCTAAA AGGCCTGTTC CTCCGAGGCC CAAAGCCCGG CTCCCTGGAC
    AGTCATGCTG CTGGGCGGCC CCTGCCCCGG CCCTCCGTTA GCCAGCGGCT CCTGCGGCGC
    ACGGCCAGCG CCCCGACCAA GAGTCAGAAG CCAGGCCGCA AGGCCTTCCC AGAGCTGGTC
    CTGGGCCTGC AGGACACCGG CTCTGAGGGG GAGGCTGGCG ATGTGGCACC CTCCAGCCCT
    GGCCCCATCC CGGAGGCCCC AACCCGGGAG GAGCCAGGCA GCCGCAGCCC CCGAGAGCCC
    CGCCCCTTCT CCATGCAGAG GTCGGTCTCC TCGCTGTGCA GCCTGGAAAC CATTGCCGAG
    GAGCCAGCCC TGGGTCCCGG CCCCCTACTG CCAGGGGCAA CCCCCCCCAG CCATGCCCCT
    GGAGGGCCCC AGTGCTCTAC AGGGCTCAGC ACCCTCTCGG CGAGCCCCCC AAGGGTGGCT
    GTGGAAGCTC CCAGGCCCCT CCAGCTCCAG ACAGGGAGCC ACGCGAGCGC TGAGCCAGCC
    CCGGGAAGCA GGCAGTGGGC ATGCAGCACT GGGGGCCCTG CTGGGGCCTG CAAGGGGGCC
    CCCAGCGGCC ATATAGAAGG CAAGGGCCAC CCCCGGGCTC TGGGCCATGT GCCCAGAAGG
    GCCAAGAGCG AGGGGCAGGT GCCCTCGGAG TCTCTGGGTG GACGGTGGCC CCTGGCCGGG
    CCCTGCCCCA CCGTGTCGTT GGACGCCACT GGCGGGGACC GGCTATGGCA GCGGCTGGAG
    CCAGGCAGCC ACCGCGGCAG CGTGTCCTCG TCCTCCAGCC TGTCGTCCAG TGACACGGTC
    ATCGAGCTCG CCCTGCCGGG CCTGGGCCTG GGCCTGGGCT GCGACAGCCT CCCAGGGACC
    CTGGCAGGAC GTCTGCCCCT GCGGCCCTGC TCGGCCCCAG TCGCCTGCCT GGACCCACCC
    GCTGTGACCA AGAGCAAATC CAACCCCAAC CTGCGGGCTG CTGGCCAGCT GCCTGTGGGG
    CCGGACAGGC TGCAGCCCCT GCCCCTGGCC CCGGGGCCAC CCTGGCGCCG CCTCCCCCTG
    GCAGGCCTCC CGGACTGCAC TGTGGCTGCC AAGTCTAAGA GCCTCGGGGA CCTGACTGCA
    GACGACTTTG CTCCCCGCGT GGAAAGCCTG GGCCGCGGCC TGGGCCTGGC TGGAGAGAGG
    CCGGCTGGGC GAAGGGTGCG GCCGGATGCC CTGACAGAGC AGTTGCGCTG GCTCACGGGG
    TTCCAGCAGG CTGGGGACAT CACTTCACCT ACCAGCCTGG GCCCCACCGG GGAGGGGGTG
    GCAGGTGCCC CGGGCTTCCT GCGGCGCTCC TCCTCCCGCA GCCAGAGCCG TGTGCGTGCC
    ATCGCCAGCC GGGCCCGCCA GGCTCAGGAG CGGCAGCAGC GGCTGCGGGG TCCACGGGGA
    CCCCCGGAGG AGGAGCGGGG CACCCCTGAG GGCGCCTGCT CCGTGGGCCA AGAGGGTTGT
    GGTGATGTGC AGGCCCCCTC CAAGGGCGCC ACCCACCAGC CCTTAGGTGC TGCCGCCACC
    GGCCTTCTGC TCCGATTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49026
    Clone ID Related Accession (Same CDS sequence) XM_010823135.2 , XM_010813415.2
    Accession Version XM_010823135.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGAG CCCCGCCCCT TCTCCATGCA GAGGTCGGTC
    TCCTCGCTGT GCAGCCTGGA AACCATTGCC GAGGAGCCAG CCCTGGGTCC CGGCCCCCTA
    CTGCCAGGGG CAACCCCCCC CAGCCATGCC CCTGGAGGGC CCCAGTGCTC TACAGGGCTC
    AGCACCCTCT CGGCGAGCCC CCCAAGGGTG GCTGTGGAAG CTCCCAGGCC CCTCCAGCTC
    CAGACAGGGA GCCACGCGAG CGCTGAGCCA GCCCCGGGAA GCAGGCAGTG GGCATGCAGC
    ACTGGGGGCC CTGCTGGGGC CTGCAAGGGG GCCCCCAGCG GCCATATAGA AGGCAAGGGC
    CACCCCCGGG CTCTGGGCCA TGTGCCCAGA AGGGCCAAGA GCGAGGGGCA GGTGCCCTCG
    GAGTCTCTGG GTGGACGGTG GCCCCTGGCC GGGCCCTGCC CCACCGTGTC GTTGGACGCC
    ACTGGCGGGG ACCGGCTATG GCAGCGGCTG GAGCCAGGCA GCCACCGCGG CAGCGTGTCC
    TCGTCCTCCA GCCTGTCGTC CAGTGACACG GTCATCGAGC TCGCCCTGCC GGGCCTGGGC
    CTGGGCCTGG GCTGCGACAG CCTCCCAGGG ACCCTGGCAG GACGTCTGCC CCTGCGGCCC
    TGCTCGGCCC CAGTCGCCTG CCTGGACCCA CCCGCTGTGA CCAAGAGCAA ATCCAACCCC
    AACCTGCGGG CTGCTGGCCA GCTGCCTGTG GGGCCGGACA GGCTGCAGCC CCTGCCCCTG
    GCCCCGGGGC CACCCTGGCG CCGCCTCCCC CTGGCAGGCC TCCCGGACTG CACTGTGGCT
    GCCAAGTCTA AGAGCCTCGG GGACCTGACT GCAGACGACT TTGCTCCCCG CGTGGAAAGC
    CTGGGCCGCG GCCTGGGCCT GGCTGGAGAG AGGCCGGCTG GGCGAAGGGT GCGGCCGGAT
    GCCCTGACAG AGCAGTTGCG CTGGCTCACG GGGTTCCAGC AGGCTGGGGA CATCACTTCA
    CCTACCAGCC TGGGCCCCAC CGGGGAGGGG GTGGCAGGTG CCCCGGGCTT CCTGCGGCGC
    TCCTCCTCCC GCAGCCAGAG CCGTGTGCGT GCCATCGCCA GCCGGGCCCG CCAGGCTCAG
    GAGCGGCAGC AGCGGCTGCG GGGTCCACGG GGACCCCCGG AGGAGGAGCG GGGCACCCCT
    GAGGGCGCCT GCTCCGTGGG CCAAGAGGGT TGTGGTGATG TGCAGGCCCC CTCCAAGGGC
    GCCACCCACC AGCCCTTAGG TGCTGCCGCC ACCGGCCTTC TGCTCCGATT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821437.1
    CDS350..4603
    Translation

    Target ORF information:

    RefSeq Version XM_010823135.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823135.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGAG CCCCGCCCCT TCTCCATGCA GAGGTCGGTC
    TCCTCGCTGT GCAGCCTGGA AACCATTGCC GAGGAGCCAG CCCTGGGTCC CGGCCCCCTA
    CTGCCAGGGG CAACCCCCCC CAGCCATGCC CCTGGAGGGC CCCAGTGCTC TACAGGGCTC
    AGCACCCTCT CGGCGAGCCC CCCAAGGGTG GCTGTGGAAG CTCCCAGGCC CCTCCAGCTC
    CAGACAGGGA GCCACGCGAG CGCTGAGCCA GCCCCGGGAA GCAGGCAGTG GGCATGCAGC
    ACTGGGGGCC CTGCTGGGGC CTGCAAGGGG GCCCCCAGCG GCCATATAGA AGGCAAGGGC
    CACCCCCGGG CTCTGGGCCA TGTGCCCAGA AGGGCCAAGA GCGAGGGGCA GGTGCCCTCG
    GAGTCTCTGG GTGGACGGTG GCCCCTGGCC GGGCCCTGCC CCACCGTGTC GTTGGACGCC
    ACTGGCGGGG ACCGGCTATG GCAGCGGCTG GAGCCAGGCA GCCACCGCGG CAGCGTGTCC
    TCGTCCTCCA GCCTGTCGTC CAGTGACACG GTCATCGAGC TCGCCCTGCC GGGCCTGGGC
    CTGGGCCTGG GCTGCGACAG CCTCCCAGGG ACCCTGGCAG GACGTCTGCC CCTGCGGCCC
    TGCTCGGCCC CAGTCGCCTG CCTGGACCCA CCCGCTGTGA CCAAGAGCAA ATCCAACCCC
    AACCTGCGGG CTGCTGGCCA GCTGCCTGTG GGGCCGGACA GGCTGCAGCC CCTGCCCCTG
    GCCCCGGGGC CACCCTGGCG CCGCCTCCCC CTGGCAGGCC TCCCGGACTG CACTGTGGCT
    GCCAAGTCTA AGAGCCTCGG GGACCTGACT GCAGACGACT TTGCTCCCCG CGTGGAAAGC
    CTGGGCCGCG GCCTGGGCCT GGCTGGAGAG AGGCCGGCTG GGCGAAGGGT GCGGCCGGAT
    GCCCTGACAG AGCAGTTGCG CTGGCTCACG GGGTTCCAGC AGGCTGGGGA CATCACTTCA
    CCTACCAGCC TGGGCCCCAC CGGGGAGGGG GTGGCAGGTG CCCCGGGCTT CCTGCGGCGC
    TCCTCCTCCC GCAGCCAGAG CCGTGTGCGT GCCATCGCCA GCCGGGCCCG CCAGGCTCAG
    GAGCGGCAGC AGCGGCTGCG GGGTCCACGG GGACCCCCGG AGGAGGAGCG GGGCACCCCT
    GAGGGCGCCT GCTCCGTGGG CCAAGAGGGT TGTGGTGATG TGCAGGCCCC CTCCAAGGGC
    GCCACCCACC AGCCCTTAGG TGCTGCCGCC ACCGGCCTTC TGCTCCGATT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49026
    Clone ID Related Accession (Same CDS sequence) XM_010823135.2 , XM_010813415.2
    Accession Version XM_010813415.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGAG CCCCGCCCCT TCTCCATGCA GAGGTCGGTC
    TCCTCGCTGT GCAGCCTGGA AACCATTGCC GAGGAGCCAG CCCTGGGTCC CGGCCCCCTA
    CTGCCAGGGG CAACCCCCCC CAGCCATGCC CCTGGAGGGC CCCAGTGCTC TACAGGGCTC
    AGCACCCTCT CGGCGAGCCC CCCAAGGGTG GCTGTGGAAG CTCCCAGGCC CCTCCAGCTC
    CAGACAGGGA GCCACGCGAG CGCTGAGCCA GCCCCGGGAA GCAGGCAGTG GGCATGCAGC
    ACTGGGGGCC CTGCTGGGGC CTGCAAGGGG GCCCCCAGCG GCCATATAGA AGGCAAGGGC
    CACCCCCGGG CTCTGGGCCA TGTGCCCAGA AGGGCCAAGA GCGAGGGGCA GGTGCCCTCG
    GAGTCTCTGG GTGGACGGTG GCCCCTGGCC GGGCCCTGCC CCACCGTGTC GTTGGACGCC
    ACTGGCGGGG ACCGGCTATG GCAGCGGCTG GAGCCAGGCA GCCACCGCGG CAGCGTGTCC
    TCGTCCTCCA GCCTGTCGTC CAGTGACACG GTCATCGAGC TCGCCCTGCC GGGCCTGGGC
    CTGGGCCTGG GCTGCGACAG CCTCCCAGGG ACCCTGGCAG GACGTCTGCC CCTGCGGCCC
    TGCTCGGCCC CAGTCGCCTG CCTGGACCCA CCCGCTGTGA CCAAGAGCAA ATCCAACCCC
    AACCTGCGGG CTGCTGGCCA GCTGCCTGTG GGGCCGGACA GGCTGCAGCC CCTGCCCCTG
    GCCCCGGGGC CACCCTGGCG CCGCCTCCCC CTGGCAGGCC TCCCGGACTG CACTGTGGCT
    GCCAAGTCTA AGAGCCTCGG GGACCTGACT GCAGACGACT TTGCTCCCCG CGTGGAAAGC
    CTGGGCCGCG GCCTGGGCCT GGCTGGAGAG AGGCCGGCTG GGCGAAGGGT GCGGCCGGAT
    GCCCTGACAG AGCAGTTGCG CTGGCTCACG GGGTTCCAGC AGGCTGGGGA CATCACTTCA
    CCTACCAGCC TGGGCCCCAC CGGGGAGGGG GTGGCAGGTG CCCCGGGCTT CCTGCGGCGC
    TCCTCCTCCC GCAGCCAGAG CCGTGTGCGT GCCATCGCCA GCCGGGCCCG CCAGGCTCAG
    GAGCGGCAGC AGCGGCTGCG GGGTCCACGG GGACCCCCGG AGGAGGAGCG GGGCACCCCT
    GAGGGCGCCT GCTCCGTGGG CCAAGAGGGT TGTGGTGATG TGCAGGCCCC CTCCAAGGGC
    GCCACCCACC AGCCCTTAGG TGCTGCCGCC ACCGGCCTTC TGCTCCGATT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811717.1
    CDS350..4603
    Translation

    Target ORF information:

    RefSeq Version XM_010813415.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813415.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCCGTCC CCTGGCCACT TACCCCAAGC CGGACCAAGG GAACCGTAGC CTGCCTGGCG 
    GAGGCCCTCC TCTGGGTCGG CGGGAGCGTG GCTGTGTCTC CGCAGTGGCA GCTTGGCCCA
    CTAGTGGAGC GATGTATGAG TGCCATGCAG GCGGGGACGC AGATGGTGAA GCTCCGGGGC
    AGCTCCAAGG GCCTGGTCCG CTTCTACTTC CTGGACGAGC ATCGCTCCTG CATCCGCTGG
    CGGCCCTCAC GCAAGAATGA GAAAGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTTAGC
    GAGGGGCGGC AGTCGGAGAT CTTCCAGCGC TACCCTGATG GCAGCTTCGA CCCCAACTGC
    TGCTTCAGCA TCTACCACGG CAGCCACCGG GAGTCGCTGG ACCTGGTCTC ACCCAGCGGC
    GACGAGGCGC GCACCTGGGT CACGGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG
    GACAGCCTGG CCCGCCGCCA GCGCACCCGG GACCAGTGGC TGAAGCAGAC ATTTGACGAG
    GCCGACAAGA ATGGGGATGG CAGCCTGAGC ATTGGCGAGG TTCTGCAGCT GCTTCACAAG
    CTGAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAAGGAGGC GGACACGGAT
    GACCACCAGG GGACACTAGG CTTTGAGGAG TTCTGTGCCT TCTACAAGAT GATGTCTACA
    CGCCGGGACC TCTACCTACT CATGCTCACC TACAGCAACC ACAAGGACCA CCTGGACGCC
    ACTGACCTGC TGCGCTTCCT GGAGGTGGAG CAGAAGATGA CGGGCGTGAC CCTTGAGAGC
    TGTCGCGACA TCATCGAGCA GTTTGAACCC TGCCCAGAGA ACAAGAGTAA AGGGGCAATG
    GGCATTGACG GCTTCACCAA CTACACGCGG AGCCCCGCCG GCGACATCTT CAACCCGGAG
    CACCGCCTCG TGCACCAGGA TATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCT
    CACAACACCT ACCTCGCGGG CGACCAGCTC ACGTCCCAGT CGAGGGCGGA CATGTACGCC
    CGGGTCCTGC AGGCCGGCTG CCGCTGTGTG GAGGTGGACT GCTGGGACGG GCCTGATGGG
    GAGCCCATCG TGCACCATGG CTACACGCTG ACCTCCAAGA TCCTCTTCAA AGATGTCATC
    GAAACCATCA ACAAGTACGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATTGAG
    AACCACTGCA GTGTCATCCA GCAGAAGAAG ATGGCCCAGT ATCTGACTGA CATTCTTGGG
    GACAAGCTGG ACCTGTCATC AGTGAGCAGC GAGGATGCCA CCATGCTGCC CTCTCCCCAG
    GTGCTCAAGG GCAAGATCCT GGTGAAGGGC AAGAAGCTCC CAGCCAACAT CAGTGAGGAT
    GCTGAGGAGG GCGAAGTGTC TGATGAGGAC AGCGCAGATG AGATCGATGA GGACTGCAAG
    CTCCTCAATG GGGATGCCTC CACCAACCGG AAACGTGTGG AAAACATTGC CAAGAGGAAA
    CTGGATTCCC TAATGAAGGA GTCCAAGATT CGGGACTGTG AAGACCCCAG CGACTTCACC
    GTGTCCACGC TGCCCCCACC TGGCAAACTT GGGCCCAAGG CAGAGGCCAA AAAGTTCGAG
    GAGGACGTGG AGATCGGGGA AGAGGCCGGA ACCAGCCGCC GGAATAGCCG GCTCCTCATG
    GGCAGCTTCT CCAAGCGCAA GAAAAAGAGC AGCAAGCTGA AGAAGGCGGC CAGTGTGGAG
    GAGGGGGACG AGGACCTGGA CTCCCAAGGC AGCCAGAGCA GGGGGGCCGC TAGGCAGAAG
    AAGACTATGA AGCTGTCTCG GGCCCTCTCA GACCTGGTCA AGTACACCAA GTCTGTGGGC
    ACCCACGACG TGGAGACGGA GGTGGCGTCC AGCTGGCAGG TGTCCTCCTT CAGCGAGACC
    AAGGCTCAGC AGATCTTGCA GCAGAAGCCG GCGCAGTACT TACGCTTCAA CCAGCACCAG
    CTCTCGCGCA TCTACCCCTC CTCCTACCGC GTGGACTCCA GCAACTACAA CCCGCAGCCC
    TTCTGGAACG CCGGCTGCCA GATGGTCGCC CTGAACTACC AGTCGGAGGG GCGGATGCTG
    CAGCTGAACC GGGCCAAGTT CAGCGCCAAC GGCAGCTGCG GCTACGTGCT CAAGCCTCAG
    TGCATGTGCC AGGGCATCTT CAACCCTAAC TCTGAGGACC CCCTGCCCGG GCAGCTCAAG
    AAGCAGCTGG TGCTGCGGAT CATCAGTGGT CAGCAGCTGC CCAAGCCGCG GGACTCGATG
    CTGGGGGACC GAGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT CGGGCTCCCG
    GTGGACTGCA ACAAGGAGCA GACCCGGGTG GTGGACGACA ATGGATTCAA CCCCATGTGG
    GAGGAGACCC TGGTGTTCAC GGTACACATG CCTGAGATAG CGCTAGTACG CTTCCTCGTC
    TGGGACCACG ACCCCATTGG GCGTGACTTC ATCGGCCAAA GGACGCTGGC CTTCAGCAGC
    ATGATGCCAG GCTATCGGCA CGTGTACCTG GAGGGGATGG AAGAAGCCTC TATCTTTGTC
    CATGTGGCCG TCAGTGACAT CAGTGGTAAG GTCAAGCAGG CTCTGGGCCT AAAAGGCCTG
    TTCCTCCGAG GCCCAAAGCC CGGCTCCCTG GACAGTCATG CTGCTGGGCG GCCCCTGCCC
    CGGCCCTCCG TTAGCCAGCG GCTCCTGCGG CGCACGGCCA GCGCCCCGAC CAAGAGTCAG
    AAGCCAGGCC GCAAGGCCTT CCCAGAGCTG GTCCTGGGCC TGCAGGACAC CGGCTCTGAG
    GGGGAGGCTG GCGATGTGGC ACCCTCCAGC CCTGGCCCCA TCCCGGAGGC CCCAACCCGG
    GAGGAGCCAG GCAGCCGCAG CCCCCGAGAG CCCCGCCCCT TCTCCATGCA GAGGTCGGTC
    TCCTCGCTGT GCAGCCTGGA AACCATTGCC GAGGAGCCAG CCCTGGGTCC CGGCCCCCTA
    CTGCCAGGGG CAACCCCCCC CAGCCATGCC CCTGGAGGGC CCCAGTGCTC TACAGGGCTC
    AGCACCCTCT CGGCGAGCCC CCCAAGGGTG GCTGTGGAAG CTCCCAGGCC CCTCCAGCTC
    CAGACAGGGA GCCACGCGAG CGCTGAGCCA GCCCCGGGAA GCAGGCAGTG GGCATGCAGC
    ACTGGGGGCC CTGCTGGGGC CTGCAAGGGG GCCCCCAGCG GCCATATAGA AGGCAAGGGC
    CACCCCCGGG CTCTGGGCCA TGTGCCCAGA AGGGCCAAGA GCGAGGGGCA GGTGCCCTCG
    GAGTCTCTGG GTGGACGGTG GCCCCTGGCC GGGCCCTGCC CCACCGTGTC GTTGGACGCC
    ACTGGCGGGG ACCGGCTATG GCAGCGGCTG GAGCCAGGCA GCCACCGCGG CAGCGTGTCC
    TCGTCCTCCA GCCTGTCGTC CAGTGACACG GTCATCGAGC TCGCCCTGCC GGGCCTGGGC
    CTGGGCCTGG GCTGCGACAG CCTCCCAGGG ACCCTGGCAG GACGTCTGCC CCTGCGGCCC
    TGCTCGGCCC CAGTCGCCTG CCTGGACCCA CCCGCTGTGA CCAAGAGCAA ATCCAACCCC
    AACCTGCGGG CTGCTGGCCA GCTGCCTGTG GGGCCGGACA GGCTGCAGCC CCTGCCCCTG
    GCCCCGGGGC CACCCTGGCG CCGCCTCCCC CTGGCAGGCC TCCCGGACTG CACTGTGGCT
    GCCAAGTCTA AGAGCCTCGG GGACCTGACT GCAGACGACT TTGCTCCCCG CGTGGAAAGC
    CTGGGCCGCG GCCTGGGCCT GGCTGGAGAG AGGCCGGCTG GGCGAAGGGT GCGGCCGGAT
    GCCCTGACAG AGCAGTTGCG CTGGCTCACG GGGTTCCAGC AGGCTGGGGA CATCACTTCA
    CCTACCAGCC TGGGCCCCAC CGGGGAGGGG GTGGCAGGTG CCCCGGGCTT CCTGCGGCGC
    TCCTCCTCCC GCAGCCAGAG CCGTGTGCGT GCCATCGCCA GCCGGGCCCG CCAGGCTCAG
    GAGCGGCAGC AGCGGCTGCG GGGTCCACGG GGACCCCCGG AGGAGGAGCG GGGCACCCCT
    GAGGGCGCCT GCTCCGTGGG CCAAGAGGGT TGTGGTGATG TGCAGGCCCC CTCCAAGGGC
    GCCACCCACC AGCCCTTAGG TGCTGCCGCC ACCGGCCTTC TGCTCCGATT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49027
    Clone ID Related Accession (Same CDS sequence) XM_010813416.1 , XM_010823136.2 , XM_010813416.2
    Accession Version XM_010813416.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACCCT CGCCCGAGGA CCCGGGCCCT GATCTGGAGA GCAGACGGTC TTTCCTGAGC 
    ACCAACATCG TGCCCGTGGT GGAGCGATGT ATGAGTGCCA TGCAGGCGGG GACGCAGATG
    GTGAAGCTCC GGGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC
    TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAAG CCAAGATCTC CATCGACTCC
    ATCCAGGAGG TTAGCGAGGG GCGGCAGTCG GAGATCTTCC AGCGCTACCC TGATGGCAGC
    TTCGACCCCA ACTGCTGCTT CAGCATCTAC CACGGCAGCC ACCGGGAGTC GCTGGACCTG
    GTCTCACCCA GCGGCGACGA GGCGCGCACC TGGGTCACGG GCCTGCGCTA CCTCATGGCC
    GGCATCAGCG ACGAGGACAG CCTGGCCCGC CGCCAGCGCA CCCGGGACCA GTGGCTGAAG
    CAGACATTTG ACGAGGCCGA CAAGAATGGG GATGGCAGCC TGAGCATTGG CGAGGTTCTG
    CAGCTGCTTC ACAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG
    GAGGCGGACA CGGATGACCA CCAGGGGACA CTAGGCTTTG AGGAGTTCTG TGCCTTCTAC
    AAGATGATGT CTACACGCCG GGACCTCTAC CTACTCATGC TCACCTACAG CAACCACAAG
    GACCACCTGG ACGCCACTGA CCTGCTGCGC TTCCTGGAGG TGGAGCAGAA GATGACGGGC
    GTGACCCTTG AGAGCTGTCG CGACATCATC GAGCAGTTTG AACCCTGCCC AGAGAACAAG
    AGTAAAGGGG CAATGGGCAT TGACGGCTTC ACCAACTACA CGCGGAGCCC CGCCGGCGAC
    ATCTTCAACC CGGAGCACCG CCTCGTGCAC CAGGATATGA CGCAGCCGCT GAGCCACTAC
    TTCATCACCT CGTCTCACAA CACCTACCTC GCGGGCGACC AGCTCACGTC CCAGTCGAGG
    GCGGACATGT ACGCCCGGGT CCTGCAGGCC GGCTGCCGCT GTGTGGAGGT GGACTGCTGG
    GACGGGCCTG ATGGGGAGCC CATCGTGCAC CATGGCTACA CGCTGACCTC CAAGATCCTC
    TTCAAAGATG TCATCGAAAC CATCAACAAG TACGCCTTCA TCAAGAATGA GTACCCAGTG
    ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAGCAGA AGAAGATGGC CCAGTATCTG
    ACTGACATTC TTGGGGACAA GCTGGACCTG TCATCAGTGA GCAGCGAGGA TGCCACCATG
    CTGCCCTCTC CCCAGGTGCT CAAGGGCAAG ATCCTGGTGA AGGGCAAGAA GCTCCCAGCC
    AACATCAGTG AGGATGCTGA GGAGGGCGAA GTGTCTGATG AGGACAGCGC AGATGAGATC
    GATGAGGACT GCAAGCTCCT CAATGGGGAT GCCTCCACCA ACCGGAAACG TGTGGAAAAC
    ATTGCCAAGA GGAAACTGGA TTCCCTAATG AAGGAGTCCA AGATTCGGGA CTGTGAAGAC
    CCCAGCGACT TCACCGTGTC CACGCTGCCC CCACCTGGCA AACTTGGGCC CAAGGCAGAG
    GCCAAAAAGT TCGAGGAGGA CGTGGAGATC GGGGAAGAGG CCGGAACCAG CCGCCGGAAT
    AGCCGGCTCC TCATGGGCAG CTTCTCCAAG CGCAAGAAAA AGAGCAGCAA GCTGAAGAAG
    GCGGCCAGTG TGGAGGAGGG GGACGAGGAC CTGGACTCCC AAGGCAGCCA GAGCAGGGGG
    GCCGCTAGGC AGAAGAAGAC TATGAAGCTG TCTCGGGCCC TCTCAGACCT GGTCAAGTAC
    ACCAAGTCTG TGGGCACCCA CGACGTGGAG ACGGAGGTGG CGTCCAGCTG GCAGGTGTCC
    TCCTTCAGCG AGACCAAGGC TCAGCAGATC TTGCAGCAGA AGCCGGCGCA GTACTTACGC
    TTCAACCAGC ACCAGCTCTC GCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC
    TACAACCCGC AGCCCTTCTG GAACGCCGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCG
    GAGGGGCGGA TGCTGCAGCT GAACCGGGCC AAGTTCAGCG CCAACGGCAG CTGCGGCTAC
    GTGCTCAAGC CTCAGTGCAT GTGCCAGGGC ATCTTCAACC CTAACTCTGA GGACCCCCTG
    CCCGGGCAGC TCAAGAAGCA GCTGGTGCTG CGGATCATCA GTGGTCAGCA GCTGCCCAAG
    CCGCGGGACT CGATGCTGGG GGACCGAGGG GAGATCATCG ACCCCTTCGT GGAGGTGGAG
    GTCATCGGGC TCCCGGTGGA CTGCAACAAG GAGCAGACCC GGGTGGTGGA CGACAATGGA
    TTCAACCCCA TGTGGGAGGA GACCCTGGTG TTCACGGTAC ACATGCCTGA GATAGCGCTA
    GTACGCTTCC TCGTCTGGGA CCACGACCCC ATTGGGCGTG ACTTCATCGG CCAAAGGACG
    CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTGGAGGG GATGGAAGAA
    GCCTCTATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG
    GGCCTAAAAG GCCTGTTCCT CCGAGGCCCA AAGCCCGGCT CCCTGGACAG TCATGCTGCT
    GGGCGGCCCC TGCCCCGGCC CTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC
    CCGACCAAGA GTCAGAAGCC AGGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCCTGCAG
    GACACCGGCT CTGAGGGGGA GGCTGGCGAT GTGGCACCCT CCAGCCCTGG CCCCATCCCG
    GAGGCCCCAA CCCGGGAGGA GCCAGGCAGC CGCAGCCCCC GAGAGCCCCG CCCCTTCTCC
    ATGCAGAGGT CGGTCTCCTC GCTGTGCAGC CTGGAAACCA TTGCCGAGGA GCCAGCCCTG
    GGTCCCGGCC CCCTACTGCC AGGGGCAACC CCCCCCAGCC ATGCCCCTGG AGGGCCCCAG
    TGCTCTACAG GGCTCAGCAC CCTCTCGGCG AGCCCCCCAA GGGTGGCTGT GGAAGCTCCC
    AGGCCCCTCC AGCTCCAGAC AGGGAGCCAC GCGAGCGCTG AGCCAGCCCC GGGAAGCAGG
    CAGTGGGCAT GCAGCACTGG GGGCCCTGCT GGGGCCTGCA AGGGGGCCCC CAGCGGCCAT
    ATAGAAGGCA AGGGCCACCC CCGGGCTCTG GGCCATGTGC CCAGAAGGGC CAAGAGCGAG
    GGGCAGGTGC CCTCGGAGTC TCTGGGTGGA CGGTGGCCCC TGGCCGGGCC CTGCCCCACC
    GTGTCGTTGG ACGCCACTGG CGGGGACCGG CTATGGCAGC GGCTGGAGCC AGGCAGCCAC
    CGCGGCAGCG TGTCCTCGTC CTCCAGCCTG TCGTCCAGTG ACACGGTCAT CGAGCTCGCC
    CTGCCGGGCC TGGGCCTGGG CCTGGGCTGC GACAGCCTCC CAGGGACCCT GGCAGGACGT
    CTGCCCCTGC GGCCCTGCTC GGCCCCAGTC GCCTGCCTGG ACCCACCCGC TGTGACCAAG
    AGCAAATCCA ACCCCAACCT GCGGGCTGCT GGCCAGCTGC CTGTGGGGCC GGACAGGCTG
    CAGCCCCTGC CCCTGGCCCC GGGGCCACCC TGGCGCCGCC TCCCCCTGGC AGGCCTCCCG
    GACTGCACTG TGGCTGCCAA GTCTAAGAGC CTCGGGGACC TGACTGCAGA CGACTTTGCT
    CCCCGCGTGG AAAGCCTGGG CCGCGGCCTG GGCCTGGCTG GAGAGAGGCC GGCTGGGCGA
    AGGGTGCGGC CGGATGCCCT GACAGAGCAG TTGCGCTGGC TCACGGGGTT CCAGCAGGCT
    GGGGACATCA CTTCACCTAC CAGCCTGGGC CCCACCGGGG AGGGGGTGGC AGGTGCCCCG
    GGCTTCCTGC GGCGCTCCTC CTCCCGCAGC CAGAGCCGTG TGCGTGCCAT CGCCAGCCGG
    GCCCGCCAGG CTCAGGAGCG GCAGCAGCGG CTGCGGGGTC CACGGGGACC CCCGGAGGAG
    GAGCGGGGCA CCCCTGAGGG CGCCTGCTCC GTGGGCCAAG AGGGTTGTGG TGATGTGCAG
    GCCCCCTCCA AGGGCGCCAC CCACCAGCCC TTAGGTGCTG CCGCCACCGG CCTTCTGCTC
    CGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811718.1
    CDS181..4389
    Translation

    Target ORF information:

    RefSeq Version XM_010813416.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACCCT CGCCCGAGGA CCCGGGCCCT GATCTGGAGA GCAGACGGTC TTTCCTGAGC 
    ACCAACATCG TGCCCGTGGT GGAGCGATGT ATGAGTGCCA TGCAGGCGGG GACGCAGATG
    GTGAAGCTCC GGGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC
    TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAAG CCAAGATCTC CATCGACTCC
    ATCCAGGAGG TTAGCGAGGG GCGGCAGTCG GAGATCTTCC AGCGCTACCC TGATGGCAGC
    TTCGACCCCA ACTGCTGCTT CAGCATCTAC CACGGCAGCC ACCGGGAGTC GCTGGACCTG
    GTCTCACCCA GCGGCGACGA GGCGCGCACC TGGGTCACGG GCCTGCGCTA CCTCATGGCC
    GGCATCAGCG ACGAGGACAG CCTGGCCCGC CGCCAGCGCA CCCGGGACCA GTGGCTGAAG
    CAGACATTTG ACGAGGCCGA CAAGAATGGG GATGGCAGCC TGAGCATTGG CGAGGTTCTG
    CAGCTGCTTC ACAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG
    GAGGCGGACA CGGATGACCA CCAGGGGACA CTAGGCTTTG AGGAGTTCTG TGCCTTCTAC
    AAGATGATGT CTACACGCCG GGACCTCTAC CTACTCATGC TCACCTACAG CAACCACAAG
    GACCACCTGG ACGCCACTGA CCTGCTGCGC TTCCTGGAGG TGGAGCAGAA GATGACGGGC
    GTGACCCTTG AGAGCTGTCG CGACATCATC GAGCAGTTTG AACCCTGCCC AGAGAACAAG
    AGTAAAGGGG CAATGGGCAT TGACGGCTTC ACCAACTACA CGCGGAGCCC CGCCGGCGAC
    ATCTTCAACC CGGAGCACCG CCTCGTGCAC CAGGATATGA CGCAGCCGCT GAGCCACTAC
    TTCATCACCT CGTCTCACAA CACCTACCTC GCGGGCGACC AGCTCACGTC CCAGTCGAGG
    GCGGACATGT ACGCCCGGGT CCTGCAGGCC GGCTGCCGCT GTGTGGAGGT GGACTGCTGG
    GACGGGCCTG ATGGGGAGCC CATCGTGCAC CATGGCTACA CGCTGACCTC CAAGATCCTC
    TTCAAAGATG TCATCGAAAC CATCAACAAG TACGCCTTCA TCAAGAATGA GTACCCAGTG
    ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAGCAGA AGAAGATGGC CCAGTATCTG
    ACTGACATTC TTGGGGACAA GCTGGACCTG TCATCAGTGA GCAGCGAGGA TGCCACCATG
    CTGCCCTCTC CCCAGGTGCT CAAGGGCAAG ATCCTGGTGA AGGGCAAGAA GCTCCCAGCC
    AACATCAGTG AGGATGCTGA GGAGGGCGAA GTGTCTGATG AGGACAGCGC AGATGAGATC
    GATGAGGACT GCAAGCTCCT CAATGGGGAT GCCTCCACCA ACCGGAAACG TGTGGAAAAC
    ATTGCCAAGA GGAAACTGGA TTCCCTAATG AAGGAGTCCA AGATTCGGGA CTGTGAAGAC
    CCCAGCGACT TCACCGTGTC CACGCTGCCC CCACCTGGCA AACTTGGGCC CAAGGCAGAG
    GCCAAAAAGT TCGAGGAGGA CGTGGAGATC GGGGAAGAGG CCGGAACCAG CCGCCGGAAT
    AGCCGGCTCC TCATGGGCAG CTTCTCCAAG CGCAAGAAAA AGAGCAGCAA GCTGAAGAAG
    GCGGCCAGTG TGGAGGAGGG GGACGAGGAC CTGGACTCCC AAGGCAGCCA GAGCAGGGGG
    GCCGCTAGGC AGAAGAAGAC TATGAAGCTG TCTCGGGCCC TCTCAGACCT GGTCAAGTAC
    ACCAAGTCTG TGGGCACCCA CGACGTGGAG ACGGAGGTGG CGTCCAGCTG GCAGGTGTCC
    TCCTTCAGCG AGACCAAGGC TCAGCAGATC TTGCAGCAGA AGCCGGCGCA GTACTTACGC
    TTCAACCAGC ACCAGCTCTC GCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC
    TACAACCCGC AGCCCTTCTG GAACGCCGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCG
    GAGGGGCGGA TGCTGCAGCT GAACCGGGCC AAGTTCAGCG CCAACGGCAG CTGCGGCTAC
    GTGCTCAAGC CTCAGTGCAT GTGCCAGGGC ATCTTCAACC CTAACTCTGA GGACCCCCTG
    CCCGGGCAGC TCAAGAAGCA GCTGGTGCTG CGGATCATCA GTGGTCAGCA GCTGCCCAAG
    CCGCGGGACT CGATGCTGGG GGACCGAGGG GAGATCATCG ACCCCTTCGT GGAGGTGGAG
    GTCATCGGGC TCCCGGTGGA CTGCAACAAG GAGCAGACCC GGGTGGTGGA CGACAATGGA
    TTCAACCCCA TGTGGGAGGA GACCCTGGTG TTCACGGTAC ACATGCCTGA GATAGCGCTA
    GTACGCTTCC TCGTCTGGGA CCACGACCCC ATTGGGCGTG ACTTCATCGG CCAAAGGACG
    CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTGGAGGG GATGGAAGAA
    GCCTCTATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG
    GGCCTAAAAG GCCTGTTCCT CCGAGGCCCA AAGCCCGGCT CCCTGGACAG TCATGCTGCT
    GGGCGGCCCC TGCCCCGGCC CTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC
    CCGACCAAGA GTCAGAAGCC AGGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCCTGCAG
    GACACCGGCT CTGAGGGGGA GGCTGGCGAT GTGGCACCCT CCAGCCCTGG CCCCATCCCG
    GAGGCCCCAA CCCGGGAGGA GCCAGGCAGC CGCAGCCCCC GAGAGCCCCG CCCCTTCTCC
    ATGCAGAGGT CGGTCTCCTC GCTGTGCAGC CTGGAAACCA TTGCCGAGGA GCCAGCCCTG
    GGTCCCGGCC CCCTACTGCC AGGGGCAACC CCCCCCAGCC ATGCCCCTGG AGGGCCCCAG
    TGCTCTACAG GGCTCAGCAC CCTCTCGGCG AGCCCCCCAA GGGTGGCTGT GGAAGCTCCC
    AGGCCCCTCC AGCTCCAGAC AGGGAGCCAC GCGAGCGCTG AGCCAGCCCC GGGAAGCAGG
    CAGTGGGCAT GCAGCACTGG GGGCCCTGCT GGGGCCTGCA AGGGGGCCCC CAGCGGCCAT
    ATAGAAGGCA AGGGCCACCC CCGGGCTCTG GGCCATGTGC CCAGAAGGGC CAAGAGCGAG
    GGGCAGGTGC CCTCGGAGTC TCTGGGTGGA CGGTGGCCCC TGGCCGGGCC CTGCCCCACC
    GTGTCGTTGG ACGCCACTGG CGGGGACCGG CTATGGCAGC GGCTGGAGCC AGGCAGCCAC
    CGCGGCAGCG TGTCCTCGTC CTCCAGCCTG TCGTCCAGTG ACACGGTCAT CGAGCTCGCC
    CTGCCGGGCC TGGGCCTGGG CCTGGGCTGC GACAGCCTCC CAGGGACCCT GGCAGGACGT
    CTGCCCCTGC GGCCCTGCTC GGCCCCAGTC GCCTGCCTGG ACCCACCCGC TGTGACCAAG
    AGCAAATCCA ACCCCAACCT GCGGGCTGCT GGCCAGCTGC CTGTGGGGCC GGACAGGCTG
    CAGCCCCTGC CCCTGGCCCC GGGGCCACCC TGGCGCCGCC TCCCCCTGGC AGGCCTCCCG
    GACTGCACTG TGGCTGCCAA GTCTAAGAGC CTCGGGGACC TGACTGCAGA CGACTTTGCT
    CCCCGCGTGG AAAGCCTGGG CCGCGGCCTG GGCCTGGCTG GAGAGAGGCC GGCTGGGCGA
    AGGGTGCGGC CGGATGCCCT GACAGAGCAG TTGCGCTGGC TCACGGGGTT CCAGCAGGCT
    GGGGACATCA CTTCACCTAC CAGCCTGGGC CCCACCGGGG AGGGGGTGGC AGGTGCCCCG
    GGCTTCCTGC GGCGCTCCTC CTCCCGCAGC CAGAGCCGTG TGCGTGCCAT CGCCAGCCGG
    GCCCGCCAGG CTCAGGAGCG GCAGCAGCGG CTGCGGGGTC CACGGGGACC CCCGGAGGAG
    GAGCGGGGCA CCCCTGAGGG CGCCTGCTCC GTGGGCCAAG AGGGTTGTGG TGATGTGCAG
    GCCCCCTCCA AGGGCGCCAC CCACCAGCCC TTAGGTGCTG CCGCCACCGG CCTTCTGCTC
    CGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49027
    Clone ID Related Accession (Same CDS sequence) XM_010813416.1 , XM_010823136.2 , XM_010813416.2
    Accession Version XM_010823136.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACCCT CGCCCGAGGA CCCGGGCCCT GATCTGGAGA GCAGACGGTC TTTCCTGAGC 
    ACCAACATCG TGCCCGTGGT GGAGCGATGT ATGAGTGCCA TGCAGGCGGG GACGCAGATG
    GTGAAGCTCC GGGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC
    TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAAG CCAAGATCTC CATCGACTCC
    ATCCAGGAGG TTAGCGAGGG GCGGCAGTCG GAGATCTTCC AGCGCTACCC TGATGGCAGC
    TTCGACCCCA ACTGCTGCTT CAGCATCTAC CACGGCAGCC ACCGGGAGTC GCTGGACCTG
    GTCTCACCCA GCGGCGACGA GGCGCGCACC TGGGTCACGG GCCTGCGCTA CCTCATGGCC
    GGCATCAGCG ACGAGGACAG CCTGGCCCGC CGCCAGCGCA CCCGGGACCA GTGGCTGAAG
    CAGACATTTG ACGAGGCCGA CAAGAATGGG GATGGCAGCC TGAGCATTGG CGAGGTTCTG
    CAGCTGCTTC ACAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG
    GAGGCGGACA CGGATGACCA CCAGGGGACA CTAGGCTTTG AGGAGTTCTG TGCCTTCTAC
    AAGATGATGT CTACACGCCG GGACCTCTAC CTACTCATGC TCACCTACAG CAACCACAAG
    GACCACCTGG ACGCCACTGA CCTGCTGCGC TTCCTGGAGG TGGAGCAGAA GATGACGGGC
    GTGACCCTTG AGAGCTGTCG CGACATCATC GAGCAGTTTG AACCCTGCCC AGAGAACAAG
    AGTAAAGGGG CAATGGGCAT TGACGGCTTC ACCAACTACA CGCGGAGCCC CGCCGGCGAC
    ATCTTCAACC CGGAGCACCG CCTCGTGCAC CAGGATATGA CGCAGCCGCT GAGCCACTAC
    TTCATCACCT CGTCTCACAA CACCTACCTC GCGGGCGACC AGCTCACGTC CCAGTCGAGG
    GCGGACATGT ACGCCCGGGT CCTGCAGGCC GGCTGCCGCT GTGTGGAGGT GGACTGCTGG
    GACGGGCCTG ATGGGGAGCC CATCGTGCAC CATGGCTACA CGCTGACCTC CAAGATCCTC
    TTCAAAGATG TCATCGAAAC CATCAACAAG TACGCCTTCA TCAAGAATGA GTACCCAGTG
    ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAGCAGA AGAAGATGGC CCAGTATCTG
    ACTGACATTC TTGGGGACAA GCTGGACCTG TCATCAGTGA GCAGCGAGGA TGCCACCATG
    CTGCCCTCTC CCCAGGTGCT CAAGGGCAAG ATCCTGGTGA AGGGCAAGAA GCTCCCAGCC
    AACATCAGTG AGGATGCTGA GGAGGGCGAA GTGTCTGATG AGGACAGCGC AGATGAGATC
    GATGAGGACT GCAAGCTCCT CAATGGGGAT GCCTCCACCA ACCGGAAACG TGTGGAAAAC
    ATTGCCAAGA GGAAACTGGA TTCCCTAATG AAGGAGTCCA AGATTCGGGA CTGTGAAGAC
    CCCAGCGACT TCACCGTGTC CACGCTGCCC CCACCTGGCA AACTTGGGCC CAAGGCAGAG
    GCCAAAAAGT TCGAGGAGGA CGTGGAGATC GGGGAAGAGG CCGGAACCAG CCGCCGGAAT
    AGCCGGCTCC TCATGGGCAG CTTCTCCAAG CGCAAGAAAA AGAGCAGCAA GCTGAAGAAG
    GCGGCCAGTG TGGAGGAGGG GGACGAGGAC CTGGACTCCC AAGGCAGCCA GAGCAGGGGG
    GCCGCTAGGC AGAAGAAGAC TATGAAGCTG TCTCGGGCCC TCTCAGACCT GGTCAAGTAC
    ACCAAGTCTG TGGGCACCCA CGACGTGGAG ACGGAGGTGG CGTCCAGCTG GCAGGTGTCC
    TCCTTCAGCG AGACCAAGGC TCAGCAGATC TTGCAGCAGA AGCCGGCGCA GTACTTACGC
    TTCAACCAGC ACCAGCTCTC GCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC
    TACAACCCGC AGCCCTTCTG GAACGCCGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCG
    GAGGGGCGGA TGCTGCAGCT GAACCGGGCC AAGTTCAGCG CCAACGGCAG CTGCGGCTAC
    GTGCTCAAGC CTCAGTGCAT GTGCCAGGGC ATCTTCAACC CTAACTCTGA GGACCCCCTG
    CCCGGGCAGC TCAAGAAGCA GCTGGTGCTG CGGATCATCA GTGGTCAGCA GCTGCCCAAG
    CCGCGGGACT CGATGCTGGG GGACCGAGGG GAGATCATCG ACCCCTTCGT GGAGGTGGAG
    GTCATCGGGC TCCCGGTGGA CTGCAACAAG GAGCAGACCC GGGTGGTGGA CGACAATGGA
    TTCAACCCCA TGTGGGAGGA GACCCTGGTG TTCACGGTAC ACATGCCTGA GATAGCGCTA
    GTACGCTTCC TCGTCTGGGA CCACGACCCC ATTGGGCGTG ACTTCATCGG CCAAAGGACG
    CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTGGAGGG GATGGAAGAA
    GCCTCTATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG
    GGCCTAAAAG GCCTGTTCCT CCGAGGCCCA AAGCCCGGCT CCCTGGACAG TCATGCTGCT
    GGGCGGCCCC TGCCCCGGCC CTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC
    CCGACCAAGA GTCAGAAGCC AGGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCCTGCAG
    GACACCGGCT CTGAGGGGGA GGCTGGCGAT GTGGCACCCT CCAGCCCTGG CCCCATCCCG
    GAGGCCCCAA CCCGGGAGGA GCCAGGCAGC CGCAGCCCCC GAGAGCCCCG CCCCTTCTCC
    ATGCAGAGGT CGGTCTCCTC GCTGTGCAGC CTGGAAACCA TTGCCGAGGA GCCAGCCCTG
    GGTCCCGGCC CCCTACTGCC AGGGGCAACC CCCCCCAGCC ATGCCCCTGG AGGGCCCCAG
    TGCTCTACAG GGCTCAGCAC CCTCTCGGCG AGCCCCCCAA GGGTGGCTGT GGAAGCTCCC
    AGGCCCCTCC AGCTCCAGAC AGGGAGCCAC GCGAGCGCTG AGCCAGCCCC GGGAAGCAGG
    CAGTGGGCAT GCAGCACTGG GGGCCCTGCT GGGGCCTGCA AGGGGGCCCC CAGCGGCCAT
    ATAGAAGGCA AGGGCCACCC CCGGGCTCTG GGCCATGTGC CCAGAAGGGC CAAGAGCGAG
    GGGCAGGTGC CCTCGGAGTC TCTGGGTGGA CGGTGGCCCC TGGCCGGGCC CTGCCCCACC
    GTGTCGTTGG ACGCCACTGG CGGGGACCGG CTATGGCAGC GGCTGGAGCC AGGCAGCCAC
    CGCGGCAGCG TGTCCTCGTC CTCCAGCCTG TCGTCCAGTG ACACGGTCAT CGAGCTCGCC
    CTGCCGGGCC TGGGCCTGGG CCTGGGCTGC GACAGCCTCC CAGGGACCCT GGCAGGACGT
    CTGCCCCTGC GGCCCTGCTC GGCCCCAGTC GCCTGCCTGG ACCCACCCGC TGTGACCAAG
    AGCAAATCCA ACCCCAACCT GCGGGCTGCT GGCCAGCTGC CTGTGGGGCC GGACAGGCTG
    CAGCCCCTGC CCCTGGCCCC GGGGCCACCC TGGCGCCGCC TCCCCCTGGC AGGCCTCCCG
    GACTGCACTG TGGCTGCCAA GTCTAAGAGC CTCGGGGACC TGACTGCAGA CGACTTTGCT
    CCCCGCGTGG AAAGCCTGGG CCGCGGCCTG GGCCTGGCTG GAGAGAGGCC GGCTGGGCGA
    AGGGTGCGGC CGGATGCCCT GACAGAGCAG TTGCGCTGGC TCACGGGGTT CCAGCAGGCT
    GGGGACATCA CTTCACCTAC CAGCCTGGGC CCCACCGGGG AGGGGGTGGC AGGTGCCCCG
    GGCTTCCTGC GGCGCTCCTC CTCCCGCAGC CAGAGCCGTG TGCGTGCCAT CGCCAGCCGG
    GCCCGCCAGG CTCAGGAGCG GCAGCAGCGG CTGCGGGGTC CACGGGGACC CCCGGAGGAG
    GAGCGGGGCA CCCCTGAGGG CGCCTGCTCC GTGGGCCAAG AGGGTTGTGG TGATGTGCAG
    GCCCCCTCCA AGGGCGCCAC CCACCAGCCC TTAGGTGCTG CCGCCACCGG CCTTCTGCTC
    CGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821438.1
    CDS181..4389
    Translation

    Target ORF information:

    RefSeq Version XM_010823136.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823136.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACCCT CGCCCGAGGA CCCGGGCCCT GATCTGGAGA GCAGACGGTC TTTCCTGAGC 
    ACCAACATCG TGCCCGTGGT GGAGCGATGT ATGAGTGCCA TGCAGGCGGG GACGCAGATG
    GTGAAGCTCC GGGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC
    TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAAG CCAAGATCTC CATCGACTCC
    ATCCAGGAGG TTAGCGAGGG GCGGCAGTCG GAGATCTTCC AGCGCTACCC TGATGGCAGC
    TTCGACCCCA ACTGCTGCTT CAGCATCTAC CACGGCAGCC ACCGGGAGTC GCTGGACCTG
    GTCTCACCCA GCGGCGACGA GGCGCGCACC TGGGTCACGG GCCTGCGCTA CCTCATGGCC
    GGCATCAGCG ACGAGGACAG CCTGGCCCGC CGCCAGCGCA CCCGGGACCA GTGGCTGAAG
    CAGACATTTG ACGAGGCCGA CAAGAATGGG GATGGCAGCC TGAGCATTGG CGAGGTTCTG
    CAGCTGCTTC ACAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG
    GAGGCGGACA CGGATGACCA CCAGGGGACA CTAGGCTTTG AGGAGTTCTG TGCCTTCTAC
    AAGATGATGT CTACACGCCG GGACCTCTAC CTACTCATGC TCACCTACAG CAACCACAAG
    GACCACCTGG ACGCCACTGA CCTGCTGCGC TTCCTGGAGG TGGAGCAGAA GATGACGGGC
    GTGACCCTTG AGAGCTGTCG CGACATCATC GAGCAGTTTG AACCCTGCCC AGAGAACAAG
    AGTAAAGGGG CAATGGGCAT TGACGGCTTC ACCAACTACA CGCGGAGCCC CGCCGGCGAC
    ATCTTCAACC CGGAGCACCG CCTCGTGCAC CAGGATATGA CGCAGCCGCT GAGCCACTAC
    TTCATCACCT CGTCTCACAA CACCTACCTC GCGGGCGACC AGCTCACGTC CCAGTCGAGG
    GCGGACATGT ACGCCCGGGT CCTGCAGGCC GGCTGCCGCT GTGTGGAGGT GGACTGCTGG
    GACGGGCCTG ATGGGGAGCC CATCGTGCAC CATGGCTACA CGCTGACCTC CAAGATCCTC
    TTCAAAGATG TCATCGAAAC CATCAACAAG TACGCCTTCA TCAAGAATGA GTACCCAGTG
    ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAGCAGA AGAAGATGGC CCAGTATCTG
    ACTGACATTC TTGGGGACAA GCTGGACCTG TCATCAGTGA GCAGCGAGGA TGCCACCATG
    CTGCCCTCTC CCCAGGTGCT CAAGGGCAAG ATCCTGGTGA AGGGCAAGAA GCTCCCAGCC
    AACATCAGTG AGGATGCTGA GGAGGGCGAA GTGTCTGATG AGGACAGCGC AGATGAGATC
    GATGAGGACT GCAAGCTCCT CAATGGGGAT GCCTCCACCA ACCGGAAACG TGTGGAAAAC
    ATTGCCAAGA GGAAACTGGA TTCCCTAATG AAGGAGTCCA AGATTCGGGA CTGTGAAGAC
    CCCAGCGACT TCACCGTGTC CACGCTGCCC CCACCTGGCA AACTTGGGCC CAAGGCAGAG
    GCCAAAAAGT TCGAGGAGGA CGTGGAGATC GGGGAAGAGG CCGGAACCAG CCGCCGGAAT
    AGCCGGCTCC TCATGGGCAG CTTCTCCAAG CGCAAGAAAA AGAGCAGCAA GCTGAAGAAG
    GCGGCCAGTG TGGAGGAGGG GGACGAGGAC CTGGACTCCC AAGGCAGCCA GAGCAGGGGG
    GCCGCTAGGC AGAAGAAGAC TATGAAGCTG TCTCGGGCCC TCTCAGACCT GGTCAAGTAC
    ACCAAGTCTG TGGGCACCCA CGACGTGGAG ACGGAGGTGG CGTCCAGCTG GCAGGTGTCC
    TCCTTCAGCG AGACCAAGGC TCAGCAGATC TTGCAGCAGA AGCCGGCGCA GTACTTACGC
    TTCAACCAGC ACCAGCTCTC GCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC
    TACAACCCGC AGCCCTTCTG GAACGCCGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCG
    GAGGGGCGGA TGCTGCAGCT GAACCGGGCC AAGTTCAGCG CCAACGGCAG CTGCGGCTAC
    GTGCTCAAGC CTCAGTGCAT GTGCCAGGGC ATCTTCAACC CTAACTCTGA GGACCCCCTG
    CCCGGGCAGC TCAAGAAGCA GCTGGTGCTG CGGATCATCA GTGGTCAGCA GCTGCCCAAG
    CCGCGGGACT CGATGCTGGG GGACCGAGGG GAGATCATCG ACCCCTTCGT GGAGGTGGAG
    GTCATCGGGC TCCCGGTGGA CTGCAACAAG GAGCAGACCC GGGTGGTGGA CGACAATGGA
    TTCAACCCCA TGTGGGAGGA GACCCTGGTG TTCACGGTAC ACATGCCTGA GATAGCGCTA
    GTACGCTTCC TCGTCTGGGA CCACGACCCC ATTGGGCGTG ACTTCATCGG CCAAAGGACG
    CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTGGAGGG GATGGAAGAA
    GCCTCTATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG
    GGCCTAAAAG GCCTGTTCCT CCGAGGCCCA AAGCCCGGCT CCCTGGACAG TCATGCTGCT
    GGGCGGCCCC TGCCCCGGCC CTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC
    CCGACCAAGA GTCAGAAGCC AGGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCCTGCAG
    GACACCGGCT CTGAGGGGGA GGCTGGCGAT GTGGCACCCT CCAGCCCTGG CCCCATCCCG
    GAGGCCCCAA CCCGGGAGGA GCCAGGCAGC CGCAGCCCCC GAGAGCCCCG CCCCTTCTCC
    ATGCAGAGGT CGGTCTCCTC GCTGTGCAGC CTGGAAACCA TTGCCGAGGA GCCAGCCCTG
    GGTCCCGGCC CCCTACTGCC AGGGGCAACC CCCCCCAGCC ATGCCCCTGG AGGGCCCCAG
    TGCTCTACAG GGCTCAGCAC CCTCTCGGCG AGCCCCCCAA GGGTGGCTGT GGAAGCTCCC
    AGGCCCCTCC AGCTCCAGAC AGGGAGCCAC GCGAGCGCTG AGCCAGCCCC GGGAAGCAGG
    CAGTGGGCAT GCAGCACTGG GGGCCCTGCT GGGGCCTGCA AGGGGGCCCC CAGCGGCCAT
    ATAGAAGGCA AGGGCCACCC CCGGGCTCTG GGCCATGTGC CCAGAAGGGC CAAGAGCGAG
    GGGCAGGTGC CCTCGGAGTC TCTGGGTGGA CGGTGGCCCC TGGCCGGGCC CTGCCCCACC
    GTGTCGTTGG ACGCCACTGG CGGGGACCGG CTATGGCAGC GGCTGGAGCC AGGCAGCCAC
    CGCGGCAGCG TGTCCTCGTC CTCCAGCCTG TCGTCCAGTG ACACGGTCAT CGAGCTCGCC
    CTGCCGGGCC TGGGCCTGGG CCTGGGCTGC GACAGCCTCC CAGGGACCCT GGCAGGACGT
    CTGCCCCTGC GGCCCTGCTC GGCCCCAGTC GCCTGCCTGG ACCCACCCGC TGTGACCAAG
    AGCAAATCCA ACCCCAACCT GCGGGCTGCT GGCCAGCTGC CTGTGGGGCC GGACAGGCTG
    CAGCCCCTGC CCCTGGCCCC GGGGCCACCC TGGCGCCGCC TCCCCCTGGC AGGCCTCCCG
    GACTGCACTG TGGCTGCCAA GTCTAAGAGC CTCGGGGACC TGACTGCAGA CGACTTTGCT
    CCCCGCGTGG AAAGCCTGGG CCGCGGCCTG GGCCTGGCTG GAGAGAGGCC GGCTGGGCGA
    AGGGTGCGGC CGGATGCCCT GACAGAGCAG TTGCGCTGGC TCACGGGGTT CCAGCAGGCT
    GGGGACATCA CTTCACCTAC CAGCCTGGGC CCCACCGGGG AGGGGGTGGC AGGTGCCCCG
    GGCTTCCTGC GGCGCTCCTC CTCCCGCAGC CAGAGCCGTG TGCGTGCCAT CGCCAGCCGG
    GCCCGCCAGG CTCAGGAGCG GCAGCAGCGG CTGCGGGGTC CACGGGGACC CCCGGAGGAG
    GAGCGGGGCA CCCCTGAGGG CGCCTGCTCC GTGGGCCAAG AGGGTTGTGG TGATGTGCAG
    GCCCCCTCCA AGGGCGCCAC CCACCAGCCC TTAGGTGCTG CCGCCACCGG CCTTCTGCTC
    CGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49027
    Clone ID Related Accession (Same CDS sequence) XM_010813416.1 , XM_010823136.2 , XM_010813416.2
    Accession Version XM_010813416.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACCCT CGCCCGAGGA CCCGGGCCCT GATCTGGAGA GCAGACGGTC TTTCCTGAGC 
    ACCAACATCG TGCCCGTGGT GGAGCGATGT ATGAGTGCCA TGCAGGCGGG GACGCAGATG
    GTGAAGCTCC GGGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC
    TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAAG CCAAGATCTC CATCGACTCC
    ATCCAGGAGG TTAGCGAGGG GCGGCAGTCG GAGATCTTCC AGCGCTACCC TGATGGCAGC
    TTCGACCCCA ACTGCTGCTT CAGCATCTAC CACGGCAGCC ACCGGGAGTC GCTGGACCTG
    GTCTCACCCA GCGGCGACGA GGCGCGCACC TGGGTCACGG GCCTGCGCTA CCTCATGGCC
    GGCATCAGCG ACGAGGACAG CCTGGCCCGC CGCCAGCGCA CCCGGGACCA GTGGCTGAAG
    CAGACATTTG ACGAGGCCGA CAAGAATGGG GATGGCAGCC TGAGCATTGG CGAGGTTCTG
    CAGCTGCTTC ACAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG
    GAGGCGGACA CGGATGACCA CCAGGGGACA CTAGGCTTTG AGGAGTTCTG TGCCTTCTAC
    AAGATGATGT CTACACGCCG GGACCTCTAC CTACTCATGC TCACCTACAG CAACCACAAG
    GACCACCTGG ACGCCACTGA CCTGCTGCGC TTCCTGGAGG TGGAGCAGAA GATGACGGGC
    GTGACCCTTG AGAGCTGTCG CGACATCATC GAGCAGTTTG AACCCTGCCC AGAGAACAAG
    AGTAAAGGGG CAATGGGCAT TGACGGCTTC ACCAACTACA CGCGGAGCCC CGCCGGCGAC
    ATCTTCAACC CGGAGCACCG CCTCGTGCAC CAGGATATGA CGCAGCCGCT GAGCCACTAC
    TTCATCACCT CGTCTCACAA CACCTACCTC GCGGGCGACC AGCTCACGTC CCAGTCGAGG
    GCGGACATGT ACGCCCGGGT CCTGCAGGCC GGCTGCCGCT GTGTGGAGGT GGACTGCTGG
    GACGGGCCTG ATGGGGAGCC CATCGTGCAC CATGGCTACA CGCTGACCTC CAAGATCCTC
    TTCAAAGATG TCATCGAAAC CATCAACAAG TACGCCTTCA TCAAGAATGA GTACCCAGTG
    ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAGCAGA AGAAGATGGC CCAGTATCTG
    ACTGACATTC TTGGGGACAA GCTGGACCTG TCATCAGTGA GCAGCGAGGA TGCCACCATG
    CTGCCCTCTC CCCAGGTGCT CAAGGGCAAG ATCCTGGTGA AGGGCAAGAA GCTCCCAGCC
    AACATCAGTG AGGATGCTGA GGAGGGCGAA GTGTCTGATG AGGACAGCGC AGATGAGATC
    GATGAGGACT GCAAGCTCCT CAATGGGGAT GCCTCCACCA ACCGGAAACG TGTGGAAAAC
    ATTGCCAAGA GGAAACTGGA TTCCCTAATG AAGGAGTCCA AGATTCGGGA CTGTGAAGAC
    CCCAGCGACT TCACCGTGTC CACGCTGCCC CCACCTGGCA AACTTGGGCC CAAGGCAGAG
    GCCAAAAAGT TCGAGGAGGA CGTGGAGATC GGGGAAGAGG CCGGAACCAG CCGCCGGAAT
    AGCCGGCTCC TCATGGGCAG CTTCTCCAAG CGCAAGAAAA AGAGCAGCAA GCTGAAGAAG
    GCGGCCAGTG TGGAGGAGGG GGACGAGGAC CTGGACTCCC AAGGCAGCCA GAGCAGGGGG
    GCCGCTAGGC AGAAGAAGAC TATGAAGCTG TCTCGGGCCC TCTCAGACCT GGTCAAGTAC
    ACCAAGTCTG TGGGCACCCA CGACGTGGAG ACGGAGGTGG CGTCCAGCTG GCAGGTGTCC
    TCCTTCAGCG AGACCAAGGC TCAGCAGATC TTGCAGCAGA AGCCGGCGCA GTACTTACGC
    TTCAACCAGC ACCAGCTCTC GCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC
    TACAACCCGC AGCCCTTCTG GAACGCCGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCG
    GAGGGGCGGA TGCTGCAGCT GAACCGGGCC AAGTTCAGCG CCAACGGCAG CTGCGGCTAC
    GTGCTCAAGC CTCAGTGCAT GTGCCAGGGC ATCTTCAACC CTAACTCTGA GGACCCCCTG
    CCCGGGCAGC TCAAGAAGCA GCTGGTGCTG CGGATCATCA GTGGTCAGCA GCTGCCCAAG
    CCGCGGGACT CGATGCTGGG GGACCGAGGG GAGATCATCG ACCCCTTCGT GGAGGTGGAG
    GTCATCGGGC TCCCGGTGGA CTGCAACAAG GAGCAGACCC GGGTGGTGGA CGACAATGGA
    TTCAACCCCA TGTGGGAGGA GACCCTGGTG TTCACGGTAC ACATGCCTGA GATAGCGCTA
    GTACGCTTCC TCGTCTGGGA CCACGACCCC ATTGGGCGTG ACTTCATCGG CCAAAGGACG
    CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTGGAGGG GATGGAAGAA
    GCCTCTATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG
    GGCCTAAAAG GCCTGTTCCT CCGAGGCCCA AAGCCCGGCT CCCTGGACAG TCATGCTGCT
    GGGCGGCCCC TGCCCCGGCC CTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC
    CCGACCAAGA GTCAGAAGCC AGGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCCTGCAG
    GACACCGGCT CTGAGGGGGA GGCTGGCGAT GTGGCACCCT CCAGCCCTGG CCCCATCCCG
    GAGGCCCCAA CCCGGGAGGA GCCAGGCAGC CGCAGCCCCC GAGAGCCCCG CCCCTTCTCC
    ATGCAGAGGT CGGTCTCCTC GCTGTGCAGC CTGGAAACCA TTGCCGAGGA GCCAGCCCTG
    GGTCCCGGCC CCCTACTGCC AGGGGCAACC CCCCCCAGCC ATGCCCCTGG AGGGCCCCAG
    TGCTCTACAG GGCTCAGCAC CCTCTCGGCG AGCCCCCCAA GGGTGGCTGT GGAAGCTCCC
    AGGCCCCTCC AGCTCCAGAC AGGGAGCCAC GCGAGCGCTG AGCCAGCCCC GGGAAGCAGG
    CAGTGGGCAT GCAGCACTGG GGGCCCTGCT GGGGCCTGCA AGGGGGCCCC CAGCGGCCAT
    ATAGAAGGCA AGGGCCACCC CCGGGCTCTG GGCCATGTGC CCAGAAGGGC CAAGAGCGAG
    GGGCAGGTGC CCTCGGAGTC TCTGGGTGGA CGGTGGCCCC TGGCCGGGCC CTGCCCCACC
    GTGTCGTTGG ACGCCACTGG CGGGGACCGG CTATGGCAGC GGCTGGAGCC AGGCAGCCAC
    CGCGGCAGCG TGTCCTCGTC CTCCAGCCTG TCGTCCAGTG ACACGGTCAT CGAGCTCGCC
    CTGCCGGGCC TGGGCCTGGG CCTGGGCTGC GACAGCCTCC CAGGGACCCT GGCAGGACGT
    CTGCCCCTGC GGCCCTGCTC GGCCCCAGTC GCCTGCCTGG ACCCACCCGC TGTGACCAAG
    AGCAAATCCA ACCCCAACCT GCGGGCTGCT GGCCAGCTGC CTGTGGGGCC GGACAGGCTG
    CAGCCCCTGC CCCTGGCCCC GGGGCCACCC TGGCGCCGCC TCCCCCTGGC AGGCCTCCCG
    GACTGCACTG TGGCTGCCAA GTCTAAGAGC CTCGGGGACC TGACTGCAGA CGACTTTGCT
    CCCCGCGTGG AAAGCCTGGG CCGCGGCCTG GGCCTGGCTG GAGAGAGGCC GGCTGGGCGA
    AGGGTGCGGC CGGATGCCCT GACAGAGCAG TTGCGCTGGC TCACGGGGTT CCAGCAGGCT
    GGGGACATCA CTTCACCTAC CAGCCTGGGC CCCACCGGGG AGGGGGTGGC AGGTGCCCCG
    GGCTTCCTGC GGCGCTCCTC CTCCCGCAGC CAGAGCCGTG TGCGTGCCAT CGCCAGCCGG
    GCCCGCCAGG CTCAGGAGCG GCAGCAGCGG CTGCGGGGTC CACGGGGACC CCCGGAGGAG
    GAGCGGGGCA CCCCTGAGGG CGCCTGCTCC GTGGGCCAAG AGGGTTGTGG TGATGTGCAG
    GCCCCCTCCA AGGGCGCCAC CCACCAGCCC TTAGGTGCTG CCGCCACCGG CCTTCTGCTC
    CGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811718.1
    CDS206..4414
    Translation

    Target ORF information:

    RefSeq Version XM_010813416.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813416.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCACCCT CGCCCGAGGA CCCGGGCCCT GATCTGGAGA GCAGACGGTC TTTCCTGAGC 
    ACCAACATCG TGCCCGTGGT GGAGCGATGT ATGAGTGCCA TGCAGGCGGG GACGCAGATG
    GTGAAGCTCC GGGGCAGCTC CAAGGGCCTG GTCCGCTTCT ACTTCCTGGA CGAGCATCGC
    TCCTGCATCC GCTGGCGGCC CTCACGCAAG AATGAGAAAG CCAAGATCTC CATCGACTCC
    ATCCAGGAGG TTAGCGAGGG GCGGCAGTCG GAGATCTTCC AGCGCTACCC TGATGGCAGC
    TTCGACCCCA ACTGCTGCTT CAGCATCTAC CACGGCAGCC ACCGGGAGTC GCTGGACCTG
    GTCTCACCCA GCGGCGACGA GGCGCGCACC TGGGTCACGG GCCTGCGCTA CCTCATGGCC
    GGCATCAGCG ACGAGGACAG CCTGGCCCGC CGCCAGCGCA CCCGGGACCA GTGGCTGAAG
    CAGACATTTG ACGAGGCCGA CAAGAATGGG GATGGCAGCC TGAGCATTGG CGAGGTTCTG
    CAGCTGCTTC ACAAGCTGAA CGTGAACCTG CCCCGGCAGA GGGTGAAGCA GATGTTCAAG
    GAGGCGGACA CGGATGACCA CCAGGGGACA CTAGGCTTTG AGGAGTTCTG TGCCTTCTAC
    AAGATGATGT CTACACGCCG GGACCTCTAC CTACTCATGC TCACCTACAG CAACCACAAG
    GACCACCTGG ACGCCACTGA CCTGCTGCGC TTCCTGGAGG TGGAGCAGAA GATGACGGGC
    GTGACCCTTG AGAGCTGTCG CGACATCATC GAGCAGTTTG AACCCTGCCC AGAGAACAAG
    AGTAAAGGGG CAATGGGCAT TGACGGCTTC ACCAACTACA CGCGGAGCCC CGCCGGCGAC
    ATCTTCAACC CGGAGCACCG CCTCGTGCAC CAGGATATGA CGCAGCCGCT GAGCCACTAC
    TTCATCACCT CGTCTCACAA CACCTACCTC GCGGGCGACC AGCTCACGTC CCAGTCGAGG
    GCGGACATGT ACGCCCGGGT CCTGCAGGCC GGCTGCCGCT GTGTGGAGGT GGACTGCTGG
    GACGGGCCTG ATGGGGAGCC CATCGTGCAC CATGGCTACA CGCTGACCTC CAAGATCCTC
    TTCAAAGATG TCATCGAAAC CATCAACAAG TACGCCTTCA TCAAGAATGA GTACCCAGTG
    ATCCTGTCCA TTGAGAACCA CTGCAGTGTC ATCCAGCAGA AGAAGATGGC CCAGTATCTG
    ACTGACATTC TTGGGGACAA GCTGGACCTG TCATCAGTGA GCAGCGAGGA TGCCACCATG
    CTGCCCTCTC CCCAGGTGCT CAAGGGCAAG ATCCTGGTGA AGGGCAAGAA GCTCCCAGCC
    AACATCAGTG AGGATGCTGA GGAGGGCGAA GTGTCTGATG AGGACAGCGC AGATGAGATC
    GATGAGGACT GCAAGCTCCT CAATGGGGAT GCCTCCACCA ACCGGAAACG TGTGGAAAAC
    ATTGCCAAGA GGAAACTGGA TTCCCTAATG AAGGAGTCCA AGATTCGGGA CTGTGAAGAC
    CCCAGCGACT TCACCGTGTC CACGCTGCCC CCACCTGGCA AACTTGGGCC CAAGGCAGAG
    GCCAAAAAGT TCGAGGAGGA CGTGGAGATC GGGGAAGAGG CCGGAACCAG CCGCCGGAAT
    AGCCGGCTCC TCATGGGCAG CTTCTCCAAG CGCAAGAAAA AGAGCAGCAA GCTGAAGAAG
    GCGGCCAGTG TGGAGGAGGG GGACGAGGAC CTGGACTCCC AAGGCAGCCA GAGCAGGGGG
    GCCGCTAGGC AGAAGAAGAC TATGAAGCTG TCTCGGGCCC TCTCAGACCT GGTCAAGTAC
    ACCAAGTCTG TGGGCACCCA CGACGTGGAG ACGGAGGTGG CGTCCAGCTG GCAGGTGTCC
    TCCTTCAGCG AGACCAAGGC TCAGCAGATC TTGCAGCAGA AGCCGGCGCA GTACTTACGC
    TTCAACCAGC ACCAGCTCTC GCGCATCTAC CCCTCCTCCT ACCGCGTGGA CTCCAGCAAC
    TACAACCCGC AGCCCTTCTG GAACGCCGGC TGCCAGATGG TCGCCCTGAA CTACCAGTCG
    GAGGGGCGGA TGCTGCAGCT GAACCGGGCC AAGTTCAGCG CCAACGGCAG CTGCGGCTAC
    GTGCTCAAGC CTCAGTGCAT GTGCCAGGGC ATCTTCAACC CTAACTCTGA GGACCCCCTG
    CCCGGGCAGC TCAAGAAGCA GCTGGTGCTG CGGATCATCA GTGGTCAGCA GCTGCCCAAG
    CCGCGGGACT CGATGCTGGG GGACCGAGGG GAGATCATCG ACCCCTTCGT GGAGGTGGAG
    GTCATCGGGC TCCCGGTGGA CTGCAACAAG GAGCAGACCC GGGTGGTGGA CGACAATGGA
    TTCAACCCCA TGTGGGAGGA GACCCTGGTG TTCACGGTAC ACATGCCTGA GATAGCGCTA
    GTACGCTTCC TCGTCTGGGA CCACGACCCC ATTGGGCGTG ACTTCATCGG CCAAAGGACG
    CTGGCCTTCA GCAGCATGAT GCCAGGCTAT CGGCACGTGT ACCTGGAGGG GATGGAAGAA
    GCCTCTATCT TTGTCCATGT GGCCGTCAGT GACATCAGTG GTAAGGTCAA GCAGGCTCTG
    GGCCTAAAAG GCCTGTTCCT CCGAGGCCCA AAGCCCGGCT CCCTGGACAG TCATGCTGCT
    GGGCGGCCCC TGCCCCGGCC CTCCGTTAGC CAGCGGCTCC TGCGGCGCAC GGCCAGCGCC
    CCGACCAAGA GTCAGAAGCC AGGCCGCAAG GCCTTCCCAG AGCTGGTCCT GGGCCTGCAG
    GACACCGGCT CTGAGGGGGA GGCTGGCGAT GTGGCACCCT CCAGCCCTGG CCCCATCCCG
    GAGGCCCCAA CCCGGGAGGA GCCAGGCAGC CGCAGCCCCC GAGAGCCCCG CCCCTTCTCC
    ATGCAGAGGT CGGTCTCCTC GCTGTGCAGC CTGGAAACCA TTGCCGAGGA GCCAGCCCTG
    GGTCCCGGCC CCCTACTGCC AGGGGCAACC CCCCCCAGCC ATGCCCCTGG AGGGCCCCAG
    TGCTCTACAG GGCTCAGCAC CCTCTCGGCG AGCCCCCCAA GGGTGGCTGT GGAAGCTCCC
    AGGCCCCTCC AGCTCCAGAC AGGGAGCCAC GCGAGCGCTG AGCCAGCCCC GGGAAGCAGG
    CAGTGGGCAT GCAGCACTGG GGGCCCTGCT GGGGCCTGCA AGGGGGCCCC CAGCGGCCAT
    ATAGAAGGCA AGGGCCACCC CCGGGCTCTG GGCCATGTGC CCAGAAGGGC CAAGAGCGAG
    GGGCAGGTGC CCTCGGAGTC TCTGGGTGGA CGGTGGCCCC TGGCCGGGCC CTGCCCCACC
    GTGTCGTTGG ACGCCACTGG CGGGGACCGG CTATGGCAGC GGCTGGAGCC AGGCAGCCAC
    CGCGGCAGCG TGTCCTCGTC CTCCAGCCTG TCGTCCAGTG ACACGGTCAT CGAGCTCGCC
    CTGCCGGGCC TGGGCCTGGG CCTGGGCTGC GACAGCCTCC CAGGGACCCT GGCAGGACGT
    CTGCCCCTGC GGCCCTGCTC GGCCCCAGTC GCCTGCCTGG ACCCACCCGC TGTGACCAAG
    AGCAAATCCA ACCCCAACCT GCGGGCTGCT GGCCAGCTGC CTGTGGGGCC GGACAGGCTG
    CAGCCCCTGC CCCTGGCCCC GGGGCCACCC TGGCGCCGCC TCCCCCTGGC AGGCCTCCCG
    GACTGCACTG TGGCTGCCAA GTCTAAGAGC CTCGGGGACC TGACTGCAGA CGACTTTGCT
    CCCCGCGTGG AAAGCCTGGG CCGCGGCCTG GGCCTGGCTG GAGAGAGGCC GGCTGGGCGA
    AGGGTGCGGC CGGATGCCCT GACAGAGCAG TTGCGCTGGC TCACGGGGTT CCAGCAGGCT
    GGGGACATCA CTTCACCTAC CAGCCTGGGC CCCACCGGGG AGGGGGTGGC AGGTGCCCCG
    GGCTTCCTGC GGCGCTCCTC CTCCCGCAGC CAGAGCCGTG TGCGTGCCAT CGCCAGCCGG
    GCCCGCCAGG CTCAGGAGCG GCAGCAGCGG CTGCGGGGTC CACGGGGACC CCCGGAGGAG
    GAGCGGGGCA CCCCTGAGGG CGCCTGCTCC GTGGGCCAAG AGGGTTGTGG TGATGTGCAG
    GCCCCCTCCA AGGGCGCCAC CCACCAGCCC TTAGGTGCTG CCGCCACCGG CCTTCTGCTC
    CGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.