PLCH2 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015297079.1	XP_015152565.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_015297080.1	XP_015152566.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_015297082.1	XP_015152568.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
XM_004947387.2	XP_004947444.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
XM_025142607.1	XP_024998375.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_004947382.2	XP_004947439.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
XM_015297082.2	XP_015152568.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_025142606.1	XP_024998374.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_025142610.1	XP_024998378.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_025142611.1	XP_024998379.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_025142612.1	XP_024998380.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
XM_025142613.1	XP_024998381.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
XM_015297075.1	XP_015152561.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_015297078.1	XP_015152564.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_015297081.1	XP_015152567.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_015297084.1	XP_015152570.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
XM_025142605.1	XP_024998373.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_015297076.1	XP_015152562.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_015297077.1	XP_015152563.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_025142608.1	XP_024998376.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_004947382.3	XP_004947439.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa147103	XM_025142613.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa147102	XM_025142612.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa18170	XM_015297084.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa18170	XM_004947387.2 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa147100	XM_025142610.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa147101	XM_025142611.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa45171	XM_015297076.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45171	XM_015297077.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45171	XM_015297075.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45171	XM_015297078.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45172	XM_015297079.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45173	XM_015297080.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa45174	XM_015297081.1 Latest version!	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa45175	XM_025142608.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa45175	XM_015297082.2 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa45175	XM_025142607.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa45175	XM_025142606.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa45175	XM_025142605.1 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa18165	XM_004947382.3 Latest version!	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OGa45175	XM_015297082.1	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa18165	XM_004947382.2	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

PLCH2 ( XM_025142613.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998381.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa147103
Clone ID Related Accession (Same CDS sequence)	XM_025142613.1
Accession Version	XM_025142613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGTGGGCTTC TCTATGTCAA 
AAAAAGTCTA CTTTTAGAAG CAGAACATTT TTAGACGCCT TGAAGGTACC CCCTCCTCAC 
TCAGGGAGGT GTAGTGACCA CCAACACACA CCTGCATGCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998381.1
CDS	660..3464
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKWASLCQKKSTFRSRTFLDALKVPPPHSGRCSDHQHTPACL

Target ORF information:

RefSeq Version	XM_025142613.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142613.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGTGGGCTTC TCTATGTCAA 
AAAAAGTCTA CTTTTAGAAG CAGAACATTT TTAGACGCCT TGAAGGTACC CCCTCCTCAC 
TCAGGGAGGT GTAGTGACCA CCAACACACA CCTGCATGCC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142612.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998380.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa147102
Clone ID Related Accession (Same CDS sequence)	XM_025142612.1
Accession Version	XM_025142612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAAAC TGAATGTCAA CCTACCCCGA CAAAAAGTCA AGCAAATGTT TAAGGAGGCT 
GATACAGACG ATAACCAGGG CACGCTAGGC TTTGATGAGT TCTGCACCTT CTACAAGATG 
ATGTCAACAC GGCGTGATTT GTACTTGCTA ATGCTCACCT ACAGCAACCA CAAGGACTTC 
CTGGATACAG ATGACCTGAG ACGCTTTCTG GAGAATGAAC AAAAGATGAC AAATGTGACC 
AGAGAACACT GCCTGGAAAT CATCAACAAG TTTGAACCAT GCCTGGAAAA CAAGAAGGCG 
GGAGCTCTGG GGATTGATGG TTTCACCAAT TACATGCGGA GTCCATCAGG GGACATTTTC 
AACCCAGAAC ACTACCAAGT GAATCAGGAC ATGAGTTACC CTTTGAGTCA CTATTTCATT 
ACCTCTTCAC ATAATACCTA CCTCATGGGA GATCAGCTGA TGTCTCAGTC TAGGGTGGAC 
ATGTATGCAT GGGTGCTACA ATCCGGCTGT CGTTGTGTAG AAGTTGACTG CTGGGATGGG 
CCGGATGGTG AACCAATTGT CCACCATGGA TACACTTTGA CTTCCAAAAT TCTCTTCAAA 
GATGTGATTG AAACTATCAA CAAATATGCC TTTATCAAGA ATGAGTACCC TGTTATCTTG 
TCAATTGAGA ACCACTGCAG TGTAGTTCAG CAGAAGAAGA TGGCCCAATA TCTTACAGAA 
ATTCTGGGTG ACAAATTGGA TCTGTCTTCT GTGCACAATG ATGACTCTAC CAGGCTCCCT 
TCACCAGCAA GTCTTAAAGG GAAGATTCTG GTTAAGGGGA AGAAGCTTCC AGCCAACATT 
AGTGATGATG CAGAGGAAGG TGAAGTTTCT GATGAGGACA GTGCTGATGA GATTGATGAT 
GACTGTAAAA TAATGAATGG AGATGCATCT ACTAATAGGA AAAGAGTGGA GAATATAGCA 
AAGAAAAAAC TTGACTCACT CATAAAAGAA TCCAAAATTC GGGACTGTGA AGATCCAAAC 
AATTTTACAG TTACCACTCT GCCATCATCT GGAAAGGCTG GTCAGAAATC AGACAGTAAG 
AAGAATAAAC TTGAGGATGA TGCAGAATCT GGGGAAGACT TCAGTACCAA TAAAAGGCAT 
AGCCGAACAT TAATGGGCAG CTTTTCTAAA CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA 
TCAACCAGTC TGGAAGAAGG TGAAGATGAT GCTGATTCTC AAGGAAATGT AGCCAGAGGC 
TCTGTACATT ACACCAGGAT AAACCGACAG AAGAAAACAA TGAAGTTGTC CCGGGCACTC 
TCAGACCTGG TGAAATATAC AAAGTCTGTC GGCATCCATG ATGTTGAAAC TGAAATCTCT 
TCCAGCTGGC AGGTTTCATC TTTCAGTGAA ACAAAAGCCC ACCAGATACT GCAGCAAAAG 
CCAGCACAGT ATCTACGTTT CAACCAACAT CAGTTATCCC GCATCTACCC ATCTTCTTAC 
CGTGTAGACT CTAGCAACTA CAATCCTCAG CCATTCTGGA ATGCTGGCTG CCAACTTGTG 
GCTTTAAACT ACCAGTCAGA GGGGAGAATG CTACAACTGA ACCGGGCCAA ATTTAGTGCC 
AATGGGAATT GTGGCTATGT CCTCAAACCA AACTGCATGT GTCAAGGCAT CTTTAATCCA 
AATTCTGAAG ATCCACTGCC TGGTCAGTTG AAGAAACAGC TGGTTCTAAG AATTATCAGT 
GGTCAACAGC TCCCAAAACC ACGGGATTCC ATGCTGGGAG ACAGAGGAGA GATCATAGAT 
CCATTTGTTG AAGTAGAAGT TATAGGCCTA CCTGTCGATT GCTTCAAAGA ACAAACTAGA 
GTAGTTGATG ATAATGGATT TAACCCAATG TGGGAAGAAA CATTGGTGTT CACACTGCAC 
ATGCCAGAGA TTGCATTGAT TCGGTTCCTT GTGTGGGATC ATGATCCAAT TGGGCGGGAT 
TTTATTGGAC AAAGAACGAT AGCTTTCAGC AGTATGATGC CAGGTTACCG ACATGTATAC 
CTAGAAGGTA TAGAAGAGGC ATCCATCTTT GTTCATGTGG CTATTAATGA CATTTGTGGT 
AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC CTATTTCTCA GAAATCCAAA GCAGGCCTCC 
CTGGACAGTC ATGCTGCTGG TCAGCTCCAT CGCAAGCATT CCTTTAGCAG CCACATCTTG 
AGACGGACAG CAAGTGCACC CACCAAAAGC CAGAAGAAGA AGAAGGGCTT TCCTGAAATT 
GTTTTTGACA CAAAAGACAA CAGCTCTGAA GGGGCCAGTG AAGACCACGA AGCAGAAGCT 
GCCTCACAGC CTTGCTTTGA GCAAGAGCCA GAGTGTGCTT CTCTGTCACC AGCTCCCAGG 
GATGGTGCCA GTGGGGAGGT ACATGGCAAA GGGTTGAAAG TAGATGTCGG CCGTTTCTTG 
ATGCAGAGGT CAGTCACATC TTTATGCAGC CTAGAAACTA TCACTGAAGA GCCTGTTATT 
GCAAATGAAA GCCATCATGC CAGCCTCTTC TTTCCTCTAC CCGTTACTCC TTACAGTGAT 
TCTGAAGAAG GCTCTGTGTC TGATCATTTC ACTGAATCTC AAACAAAGGG AAGCAACCAA 
TCGAACCGGC TTCCAGAACC TCCGCTCACT AGAGAACAAA GACAAACTTT GGCTGCAGAA 
GACCAAGCGC AAATAGCCAC AAAAGCAGCT GACATTAATG TATTAGTCCA AGATGATGAG 
AGGACAAGCA TGGCAGAACC TCTCAAAGAA GATGATGGTT GGACGCAGGC ATGCAGTGAA 
GCTGAAGATC AAGTTAATTT GGCAGCGCAT CCTCAAAAAG CGTGGCTGGT GGTGGCAGAA 
GCTAAGAGTA CTGGCCAGCC TGTTGAAAAC TGCCAGAAGC AGAATGACCA AACAGGGCAG 
ATATTGAAAG ACTTGAAAAC CACTGCTGAA TTGAAAAGCC AAAAACCTGG TGCAGCAGCT 
GTCCTGCACC GAAACAATGT GATTAATGGC ACACATCCAA CTTTGTCTCC AGATATTTTC 
CAGAAAGCCC AGGCTGCACA GGCCCTACCT GTTTCTCAGA CAAGCTCTTA CATGTTAATA 
AGGAAGTCAA AGAGTGAAGG GCTGAAAAAG TCAGACTCCT CAGCTTTAAT AAAAAATCCA 
AGTAGCCTAT CTCCAGCAGC AGAAGTATAC TTTGATGCTA CTATTAATGA CCGGATCTGG 
AGCAAGCTAG ATTGCAGCAA TCATCGTGAC AGCATGTCTT CCTCTTCCAG CATGTCCTCC 
AATGACACTG TGATAGATCT GTCTTTACCT AATCTAGCTC GCAAAAGCCT TCCAGATCTT 
GGCATCCGTC ATGAGAACTT TGAGTCCCTT GCCATGAGAA AGAGCATTCG CCCACGTTCT 
GCTACAGCGT CATGCAATGA AATGCCAATA GTGAGCAAGA GTAAATCCAA TCCTAACCTG 
AGGTCTGACC AGCTGCCAGC GGATGAATTA TGCCCCCGTC CTCTTGCAAG GAGTCCTCAG 
GATGCATTCT CATCCATTCC TAGGAGGCAT ACCTGGAGCA GGCTGTACAT GGAAAGTCTC 
AGGCAATCTT CTAACAAATC CAAAGCACAG GATAAGGTGG GGAATAACCA GGCAAAATCT 
AAGAGTCTTG GAGACCTTAC CTCTGATGAC ATTGTGTGCA CTTTTGAAAG CAAATATCGT 
AGCATCAGCA GGAGTTTTGT CACTCGATCA ATGCGTGAGC AGCGCCGCTC TGCAGGCCTG 
AGAACCTCTG TCAAACCCCA GGATGAATTG ACTGAGCAGC TGAAGAAGCT AACGGCCTTT 
CAGCAGGAAA ATGATATAAC TTCCCCCATC AGTCTTGATC CAACTGAATC TGAAGAGGAA 
GCAGAGAGCT TAGGACTCCT TCGCAGGTCA TCATCTCGCA GTCAGAGCAG GGTGCGGTAC 
ATTGCCAACA GAGCCAAGCA AGCTCAGGAG AGACAGCGAC TGCAAAGCCT GGGCCAGCTC 
AGTGGGAGTC CCATTGAAGA GAGAGGAAAT CCAGAGGGAG CCTGCAGTGT TGCAAAAGCA 
GTTTGTATGG ATATAGCATC TCCTGCTGTG TTTACATCCC AGCCTAAGAA CCAAACTGAT 
TATAATACTG ACACAGAAGT CTTCTTTATG CTGAAACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998380.1
CDS	275..4456
Translation	MHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142612.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142612.1

ORF Insert Sequence:

ATGCACAAAC TGAATGTCAA CCTACCCCGA CAAAAAGTCA AGCAAATGTT TAAGGAGGCT 
GATACAGACG ATAACCAGGG CACGCTAGGC TTTGATGAGT TCTGCACCTT CTACAAGATG 
ATGTCAACAC GGCGTGATTT GTACTTGCTA ATGCTCACCT ACAGCAACCA CAAGGACTTC 
CTGGATACAG ATGACCTGAG ACGCTTTCTG GAGAATGAAC AAAAGATGAC AAATGTGACC 
AGAGAACACT GCCTGGAAAT CATCAACAAG TTTGAACCAT GCCTGGAAAA CAAGAAGGCG 
GGAGCTCTGG GGATTGATGG TTTCACCAAT TACATGCGGA GTCCATCAGG GGACATTTTC 
AACCCAGAAC ACTACCAAGT GAATCAGGAC ATGAGTTACC CTTTGAGTCA CTATTTCATT 
ACCTCTTCAC ATAATACCTA CCTCATGGGA GATCAGCTGA TGTCTCAGTC TAGGGTGGAC 
ATGTATGCAT GGGTGCTACA ATCCGGCTGT CGTTGTGTAG AAGTTGACTG CTGGGATGGG 
CCGGATGGTG AACCAATTGT CCACCATGGA TACACTTTGA CTTCCAAAAT TCTCTTCAAA 
GATGTGATTG AAACTATCAA CAAATATGCC TTTATCAAGA ATGAGTACCC TGTTATCTTG 
TCAATTGAGA ACCACTGCAG TGTAGTTCAG CAGAAGAAGA TGGCCCAATA TCTTACAGAA 
ATTCTGGGTG ACAAATTGGA TCTGTCTTCT GTGCACAATG ATGACTCTAC CAGGCTCCCT 
TCACCAGCAA GTCTTAAAGG GAAGATTCTG GTTAAGGGGA AGAAGCTTCC AGCCAACATT 
AGTGATGATG CAGAGGAAGG TGAAGTTTCT GATGAGGACA GTGCTGATGA GATTGATGAT 
GACTGTAAAA TAATGAATGG AGATGCATCT ACTAATAGGA AAAGAGTGGA GAATATAGCA 
AAGAAAAAAC TTGACTCACT CATAAAAGAA TCCAAAATTC GGGACTGTGA AGATCCAAAC 
AATTTTACAG TTACCACTCT GCCATCATCT GGAAAGGCTG GTCAGAAATC AGACAGTAAG 
AAGAATAAAC TTGAGGATGA TGCAGAATCT GGGGAAGACT TCAGTACCAA TAAAAGGCAT 
AGCCGAACAT TAATGGGCAG CTTTTCTAAA CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA 
TCAACCAGTC TGGAAGAAGG TGAAGATGAT GCTGATTCTC AAGGAAATGT AGCCAGAGGC 
TCTGTACATT ACACCAGGAT AAACCGACAG AAGAAAACAA TGAAGTTGTC CCGGGCACTC 
TCAGACCTGG TGAAATATAC AAAGTCTGTC GGCATCCATG ATGTTGAAAC TGAAATCTCT 
TCCAGCTGGC AGGTTTCATC TTTCAGTGAA ACAAAAGCCC ACCAGATACT GCAGCAAAAG 
CCAGCACAGT ATCTACGTTT CAACCAACAT CAGTTATCCC GCATCTACCC ATCTTCTTAC 
CGTGTAGACT CTAGCAACTA CAATCCTCAG CCATTCTGGA ATGCTGGCTG CCAACTTGTG 
GCTTTAAACT ACCAGTCAGA GGGGAGAATG CTACAACTGA ACCGGGCCAA ATTTAGTGCC 
AATGGGAATT GTGGCTATGT CCTCAAACCA AACTGCATGT GTCAAGGCAT CTTTAATCCA 
AATTCTGAAG ATCCACTGCC TGGTCAGTTG AAGAAACAGC TGGTTCTAAG AATTATCAGT 
GGTCAACAGC TCCCAAAACC ACGGGATTCC ATGCTGGGAG ACAGAGGAGA GATCATAGAT 
CCATTTGTTG AAGTAGAAGT TATAGGCCTA CCTGTCGATT GCTTCAAAGA ACAAACTAGA 
GTAGTTGATG ATAATGGATT TAACCCAATG TGGGAAGAAA CATTGGTGTT CACACTGCAC 
ATGCCAGAGA TTGCATTGAT TCGGTTCCTT GTGTGGGATC ATGATCCAAT TGGGCGGGAT 
TTTATTGGAC AAAGAACGAT AGCTTTCAGC AGTATGATGC CAGGTTACCG ACATGTATAC 
CTAGAAGGTA TAGAAGAGGC ATCCATCTTT GTTCATGTGG CTATTAATGA CATTTGTGGT 
AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC CTATTTCTCA GAAATCCAAA GCAGGCCTCC 
CTGGACAGTC ATGCTGCTGG TCAGCTCCAT CGCAAGCATT CCTTTAGCAG CCACATCTTG 
AGACGGACAG CAAGTGCACC CACCAAAAGC CAGAAGAAGA AGAAGGGCTT TCCTGAAATT 
GTTTTTGACA CAAAAGACAA CAGCTCTGAA GGGGCCAGTG AAGACCACGA AGCAGAAGCT 
GCCTCACAGC CTTGCTTTGA GCAAGAGCCA GAGTGTGCTT CTCTGTCACC AGCTCCCAGG 
GATGGTGCCA GTGGGGAGGT ACATGGCAAA GGGTTGAAAG TAGATGTCGG CCGTTTCTTG 
ATGCAGAGGT CAGTCACATC TTTATGCAGC CTAGAAACTA TCACTGAAGA GCCTGTTATT 
GCAAATGAAA GCCATCATGC CAGCCTCTTC TTTCCTCTAC CCGTTACTCC TTACAGTGAT 
TCTGAAGAAG GCTCTGTGTC TGATCATTTC ACTGAATCTC AAACAAAGGG AAGCAACCAA 
TCGAACCGGC TTCCAGAACC TCCGCTCACT AGAGAACAAA GACAAACTTT GGCTGCAGAA 
GACCAAGCGC AAATAGCCAC AAAAGCAGCT GACATTAATG TATTAGTCCA AGATGATGAG 
AGGACAAGCA TGGCAGAACC TCTCAAAGAA GATGATGGTT GGACGCAGGC ATGCAGTGAA 
GCTGAAGATC AAGTTAATTT GGCAGCGCAT CCTCAAAAAG CGTGGCTGGT GGTGGCAGAA 
GCTAAGAGTA CTGGCCAGCC TGTTGAAAAC TGCCAGAAGC AGAATGACCA AACAGGGCAG 
ATATTGAAAG ACTTGAAAAC CACTGCTGAA TTGAAAAGCC AAAAACCTGG TGCAGCAGCT 
GTCCTGCACC GAAACAATGT GATTAATGGC ACACATCCAA CTTTGTCTCC AGATATTTTC 
CAGAAAGCCC AGGCTGCACA GGCCCTACCT GTTTCTCAGA CAAGCTCTTA CATGTTAATA 
AGGAAGTCAA AGAGTGAAGG GCTGAAAAAG TCAGACTCCT CAGCTTTAAT AAAAAATCCA 
AGTAGCCTAT CTCCAGCAGC AGAAGTATAC TTTGATGCTA CTATTAATGA CCGGATCTGG 
AGCAAGCTAG ATTGCAGCAA TCATCGTGAC AGCATGTCTT CCTCTTCCAG CATGTCCTCC 
AATGACACTG TGATAGATCT GTCTTTACCT AATCTAGCTC GCAAAAGCCT TCCAGATCTT 
GGCATCCGTC ATGAGAACTT TGAGTCCCTT GCCATGAGAA AGAGCATTCG CCCACGTTCT 
GCTACAGCGT CATGCAATGA AATGCCAATA GTGAGCAAGA GTAAATCCAA TCCTAACCTG 
AGGTCTGACC AGCTGCCAGC GGATGAATTA TGCCCCCGTC CTCTTGCAAG GAGTCCTCAG 
GATGCATTCT CATCCATTCC TAGGAGGCAT ACCTGGAGCA GGCTGTACAT GGAAAGTCTC 
AGGCAATCTT CTAACAAATC CAAAGCACAG GATAAGGTGG GGAATAACCA GGCAAAATCT 
AAGAGTCTTG GAGACCTTAC CTCTGATGAC ATTGTGTGCA CTTTTGAAAG CAAATATCGT 
AGCATCAGCA GGAGTTTTGT CACTCGATCA ATGCGTGAGC AGCGCCGCTC TGCAGGCCTG 
AGAACCTCTG TCAAACCCCA GGATGAATTG ACTGAGCAGC TGAAGAAGCT AACGGCCTTT 
CAGCAGGAAA ATGATATAAC TTCCCCCATC AGTCTTGATC CAACTGAATC TGAAGAGGAA 
GCAGAGAGCT TAGGACTCCT TCGCAGGTCA TCATCTCGCA GTCAGAGCAG GGTGCGGTAC 
ATTGCCAACA GAGCCAAGCA AGCTCAGGAG AGACAGCGAC TGCAAAGCCT GGGCCAGCTC 
AGTGGGAGTC CCATTGAAGA GAGAGGAAAT CCAGAGGGAG CCTGCAGTGT TGCAAAAGCA 
GTTTGTATGG ATATAGCATC TCCTGCTGTG TTTACATCCC AGCCTAAGAA CCAAACTGAT 
TATAATACTG ACACAGAAGT CTTCTTTATG CTGAAACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_004947387.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947444.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X10, mRNA.

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CloneID	OGa18170
Clone ID Related Accession (Same CDS sequence)	XM_004947387.2 , XM_015297084.1
Accession Version	XM_004947387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004947387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGT TAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947444.1
CDS	536..4285
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTVKN

Target ORF information:

RefSeq Version	XM_004947387.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004947387.2

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGT TAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297084.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152570.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa18170
Clone ID Related Accession (Same CDS sequence)	XM_004947387.2 , XM_015297084.1
Accession Version	XM_015297084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGT TAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152570.1
CDS	536..4285
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTVKN

Target ORF information:

RefSeq Version	XM_015297084.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297084.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGT TAAGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142610.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998378.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa147100
Clone ID Related Accession (Same CDS sequence)	XM_025142610.1
Accession Version	XM_025142610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATA CAAGCACCAT TAAATGCCTC AGCCCAGATA AAGTGGAAAG ATGTATGAGC 
TCCATGCAAG CTGGTACCCA AATGATCAAA CTCCGTGGTG GTTCCAAAGG GTTGGTCCGC 
TTCTATTACC TGGATGAGCA CAAGTCATGC ATTCGCTGGA GACCCTCCCG AAAGAATGAG 
AGAGCCAAAA TTTCCATCGA CTCTATTCAA GAGGTTTGTG AGGGGAAGCA ATCGGAGATC 
TTTCAGCGCT ATGCTGATGG AAGTTTTGAT CCTAACTGCT GCTTCAGCAT CTACTATGGA 
GATCATATGG AATCCCTTGA CCTGGTGTCT CCCAGTGGAG ATGAAGCACG TACTTGGATC 
ACAGGGCTAA AATATCTGAT GGCAGGGATC AGTGATGAAG ACAGCCTCTC AAAACGCCAA 
AGAACCAGAG ACCAGTGGTT GAAGCAGACA TTTGATGAAG CAGATAAAAA TGGTGATGGC 
TGTCTGAGTA TTACTGAAGT GCTCCAACTA ATGCACAAAC TGAATGTCAA CCTACCCCGA 
CAAAAAGTCA AGCAAATGTT TAAGGAGGCT GATACAGACG ATAACCAGGG CACGCTAGGC 
TTTGATGAGT TCTGCACCTT CTACAAGATG ATGTCAACAC GGCGTGATTT GTACTTGCTA 
ATGCTCACCT ACAGCAACCA CAAGGACTTC CTGGATACAG ATGACCTGAG ACGCTTTCTG 
GAGAATGAAC AAAAGATGAC AAATGTGACC AGAGAACACT GCCTGGAAAT CATCAACAAG 
TTTGAACCAT GCCTGGAAAA CAAGAAGGCG GGAGCTCTGG GGATTGATGG TTTCACCAAT 
TACATGCGGA GTCCATCAGG GGACATTTTC AACCCAGAAC ACTACCAAGT GAATCAGGAC 
ATGAGTTACC CTTTGAGTCA CTATTTCATT ACCTCTTCAC ATAATACCTA CCTCATGGGA 
GATCAGCTGA TGTCTCAGTC TAGGGTGGAC ATGTATGCAT GGGTGCTACA ATCCGGCTGT 
CGTTGTGTAG AAGTTGACTG CTGGGATGGG CCGGATGGTG AACCAATTGT CCACCATGGA 
TACACTTTGA CTTCCAAAAT TCTCTTCAAA GATGTGATTG AAACTATCAA CAAATATGCC 
TTTATCAAGA ATGAGTACCC TGTTATCTTG TCAATTGAGA ACCACTGCAG TGTAGTTCAG 
CAGAAGAAGA TGGCCCAATA TCTTACAGAA ATTCTGGGTG ACAAATTGGA TCTGTCTTCT 
GTGCACAATG ATGACTCTAC CAGGCTCCCT TCACCAGCAA GTCTTAAAGG GAAGATTCTG 
GTTAAGGGGA AGAAGCTTCC AGCCAACATT AGTGATGATG CAGAGGAAGG TGAAGTTTCT 
GATGAGGACA GTGCTGATGA GATTGATGAT GACTGTAAAA TAATGAATGG AGATGCATCT 
ACTAATAGGA AAAGAGTGGA GAATATAGCA AAGAAAAAAC TTGACTCACT CATAAAAGAA 
TCCAAAATTC GGGACTGTGA AGATCCAAAC AATTTTACAG TTACCACTCT GCCATCATCT 
GGAAAGGCTG GTCAGAAATC AGACAGTAAG AAGAATAAAC TTGAGGATGA TGCAGAATCT 
GGGGAAGACT TCAGTACCAA TAAAAGGCAT AGCCGAACAT TAATGGGCAG CTTTTCTAAA 
CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA TCAACCAGTC TGGAAGAAGG TGAAGATGAT 
GCTGATTCTC AAGGAAATGT AGCCAGAGGC TCTGTACATT ACACCAGGAT AAACCGACAG 
AAGAAAACAA TGAAGTTGTC CCGGGCACTC TCAGACCTGG TGAAATATAC AAAGTCTGTC 
GGCATCCATG ATGTTGAAAC TGAAATCTCT TCCAGCTGGC AGGTTTCATC TTTCAGTGAA 
ACAAAAGCCC ACCAGATACT GCAGCAAAAG CCAGCACAGT ATCTACGTTT CAACCAACAT 
CAGTTATCCC GCATCTACCC ATCTTCTTAC CGTGTAGACT CTAGCAACTA CAATCCTCAG 
CCATTCTGGA ATGCTGGCTG CCAACTTGTG GCTTTAAACT ACCAGTCAGA GGGGAGAATG 
CTACAACTGA ACCGGGCCAA ATTTAGTGCC AATGGGAATT GTGGCTATGT CCTCAAACCA 
AACTGCATGT GTCAAGGCAT CTTTAATCCA AATTCTGAAG ATCCACTGCC TGGTCAGTTG 
AAGAAACAGC TGGTTCTAAG AATTATCAGT GGTCAACAGC TCCCAAAACC ACGGGATTCC 
ATGCTGGGAG ACAGAGGAGA GATCATAGAT CCATTTGTTG AAGTAGAAGT TATAGGCCTA 
CCTGTCGATT GCTTCAAAGA ACAAACTAGA GTAGTTGATG ATAATGGATT TAACCCAATG 
TGGGAAGAAA CATTGGTGTT CACACTGCAC ATGCCAGAGA TTGCATTGAT TCGGTTCCTT 
GTGTGGGATC ATGATCCAAT TGGGCGGGAT TTTATTGGAC AAAGAACGAT AGCTTTCAGC 
AGTATGATGC CAGGTTACCG ACATGTATAC CTAGAAGGTA TAGAAGAGGC ATCCATCTTT 
GTTCATGTGG CTATTAATGA CATTTGTGGT AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC 
CTATTTCTCA GAAATCCAAA GCAGGCCTCC CTGGACAGTC ATGCTGCTGG TCAGCTCCAT 
CGCAAGCATT CCTTTAGCAG CCACATCTTG AGACGGACAG CAAGTGCACC CACCAAAAGC 
CAGAAGAAGA AGAAGGGCTT TCCTGAAATT GTTTTTGACA CAAAAGACAA CAGCTCTGAA 
GGGGCCAGTG AAGACCACGA AGCAGAAGCT GCCTCACAGC CTTGCTTTGA GCAAGAGCCA 
GAGTGTGCTT CTCTGTCACC AGCTCCCAGG GATGGTGCCA GTGGGGAGGT ACATGGCAAA 
GGGTTGAAAG TAGATGTCGG CCGTTTCTTG ATGCAGAGGT CAGTCACATC TTTATGCAGC 
CTAGAAACTA TCACTGAAGA GCCTGTTATT GCAAATGAAA GCCATCATGC CAGCCTCTTC 
TTTCCTCTAC CCGTTACTCC TTACAGTGAT TCTGAAGAAG GCTCTGTGTC TGATCATTTC 
ACTGAATCTC AAACAAAGGG AAGCAACCAA TCGAACCGGC TTCCAGAACC TCCGCTCACT 
AGAGAACAAA GACAAACTTT GGCTGCAGAA GACCAAGCGC AAATAGCCAC AAAAGCAGCT 
GACATTAATG TATTAGTCCA AGATGATGAG AGGACAAGCA TGGCAGAACC TCTCAAAGAA 
GATGATGGTT GGACGCAGGC ATGCAGTGAA GCTGAAGATC AAGTTAATTT GGCAGCGCAT 
CCTCAAAAAG CGTGGCTGGT GGTGGCAGAA GCTAAGAGTA CTGGCCAGCC TGTTGAAAAC 
TGCCAGAAGC AGAATGACCA AACAGGGCAG ATATTGAAAG ACTTGAAAAC CACTGCTGAA 
TTGAAAAGCC AAAAACCTGG TGCAGCAGCT GTCCTGCACC GAAACAATGT GATTAATGGC 
ACACATCCAA CTTTGTCTCC AGATATTTTC CAGAAAGCCC AGGCTGCACA GGCCCTACCT 
GTTTCTCAGA CAAGCTCTTA CATGTTAATA AGGAAGTCAA AGAGTGAAGG GCTGAAAAAG 
TCAGACTCCT CAGCTTTAAT AAAAAATCCA AGTAGCCTAT CTCCAGCAGC AGAAGTATAC 
TTTGATGCTA CTATTAATGA CCGGATCTGG AGCAAGCTAG ATTGCAGCAA TCATCGTGAC 
AGCATGTCTT CCTCTTCCAG CATGTCCTCC AATGACACTG TGATAGATCT GTCTTTACCT 
AATCTAGCTC GCAAAAGCCT TCCAGATCTT GGCATCCGTC ATGAGAACTT TGAGTCCCTT 
GCCATGAGAA AGAGCATTCG CCCACGTTCT GCTACAGCGT CATGCAATGA AATGCCAATA 
GTGAGCAAGA GTAAATCCAA TCCTAACCTG AGGTCTGACC AGCTGCCAGC GGATGAATTA 
TGCCCCCGTC CTCTTGCAAG GAGTCCTCAG GATGCATTCT CATCCATTCC TAGGAGGCAT 
ACCTGGAGCA GGCTGTACAT GGAAAGTCTC AGGCAATCTT CTAACAAATC CAAAGCACAG 
GATAAGGTGG GGAATAACCA GGCAAAATCT AAGAGTCTTG GAGACCTTAC CTCTGATGAC 
ATTGTGTGCA CTTTTGAAAG CAAATATCGT AGCATCAGCA GGAGTTTTGT CACTCGATCA 
ATGCGTGAGC AGCGCCGCTC TGCAGGCCTG AGAACCTCTG TCAAACCCCA GGATGAATTG 
ACTGAGCAGC TGAAGAAGCT AACGGCCTTT CAGCAGGAAA ATGATATAAC TTCCCCCATC 
AGTCTTGATC CAACTGAATC TGAAGAGGAA GCAGAGAGCT TAGGACTCCT TCGCAGGTCA 
TCATCTCGCA GTCAGAGCAG GGTGCGGTAC ATTGCCAACA GAGCCAAGCA AGCTCAGGAG 
AGACAGCGAC TGCAAAGCCT GGGCCAGCTC AGTGGGAGTC CCATTGAAGA GAGAGGAAAT 
CCAGAGGGAG CCTGCAGTGT TGCAAAAGCA GTTTGTATGG ATATAGCATC TCCTGCTGTG 
TTTACATCCC AGCCTAAGAA CCAAACTGAT TATAATACTG ACACAGAAGT CTTCTTTATG 
CTGAAACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998378.1
CDS	627..5318
Translation	MDDTSTIKCLSPDKVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142610.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142610.1

ORF Insert Sequence:

ATGGATGATA CAAGCACCAT TAAATGCCTC AGCCCAGATA AAGTGGAAAG ATGTATGAGC 
TCCATGCAAG CTGGTACCCA AATGATCAAA CTCCGTGGTG GTTCCAAAGG GTTGGTCCGC 
TTCTATTACC TGGATGAGCA CAAGTCATGC ATTCGCTGGA GACCCTCCCG AAAGAATGAG 
AGAGCCAAAA TTTCCATCGA CTCTATTCAA GAGGTTTGTG AGGGGAAGCA ATCGGAGATC 
TTTCAGCGCT ATGCTGATGG AAGTTTTGAT CCTAACTGCT GCTTCAGCAT CTACTATGGA 
GATCATATGG AATCCCTTGA CCTGGTGTCT CCCAGTGGAG ATGAAGCACG TACTTGGATC 
ACAGGGCTAA AATATCTGAT GGCAGGGATC AGTGATGAAG ACAGCCTCTC AAAACGCCAA 
AGAACCAGAG ACCAGTGGTT GAAGCAGACA TTTGATGAAG CAGATAAAAA TGGTGATGGC 
TGTCTGAGTA TTACTGAAGT GCTCCAACTA ATGCACAAAC TGAATGTCAA CCTACCCCGA 
CAAAAAGTCA AGCAAATGTT TAAGGAGGCT GATACAGACG ATAACCAGGG CACGCTAGGC 
TTTGATGAGT TCTGCACCTT CTACAAGATG ATGTCAACAC GGCGTGATTT GTACTTGCTA 
ATGCTCACCT ACAGCAACCA CAAGGACTTC CTGGATACAG ATGACCTGAG ACGCTTTCTG 
GAGAATGAAC AAAAGATGAC AAATGTGACC AGAGAACACT GCCTGGAAAT CATCAACAAG 
TTTGAACCAT GCCTGGAAAA CAAGAAGGCG GGAGCTCTGG GGATTGATGG TTTCACCAAT 
TACATGCGGA GTCCATCAGG GGACATTTTC AACCCAGAAC ACTACCAAGT GAATCAGGAC 
ATGAGTTACC CTTTGAGTCA CTATTTCATT ACCTCTTCAC ATAATACCTA CCTCATGGGA 
GATCAGCTGA TGTCTCAGTC TAGGGTGGAC ATGTATGCAT GGGTGCTACA ATCCGGCTGT 
CGTTGTGTAG AAGTTGACTG CTGGGATGGG CCGGATGGTG AACCAATTGT CCACCATGGA 
TACACTTTGA CTTCCAAAAT TCTCTTCAAA GATGTGATTG AAACTATCAA CAAATATGCC 
TTTATCAAGA ATGAGTACCC TGTTATCTTG TCAATTGAGA ACCACTGCAG TGTAGTTCAG 
CAGAAGAAGA TGGCCCAATA TCTTACAGAA ATTCTGGGTG ACAAATTGGA TCTGTCTTCT 
GTGCACAATG ATGACTCTAC CAGGCTCCCT TCACCAGCAA GTCTTAAAGG GAAGATTCTG 
GTTAAGGGGA AGAAGCTTCC AGCCAACATT AGTGATGATG CAGAGGAAGG TGAAGTTTCT 
GATGAGGACA GTGCTGATGA GATTGATGAT GACTGTAAAA TAATGAATGG AGATGCATCT 
ACTAATAGGA AAAGAGTGGA GAATATAGCA AAGAAAAAAC TTGACTCACT CATAAAAGAA 
TCCAAAATTC GGGACTGTGA AGATCCAAAC AATTTTACAG TTACCACTCT GCCATCATCT 
GGAAAGGCTG GTCAGAAATC AGACAGTAAG AAGAATAAAC TTGAGGATGA TGCAGAATCT 
GGGGAAGACT TCAGTACCAA TAAAAGGCAT AGCCGAACAT TAATGGGCAG CTTTTCTAAA 
CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA TCAACCAGTC TGGAAGAAGG TGAAGATGAT 
GCTGATTCTC AAGGAAATGT AGCCAGAGGC TCTGTACATT ACACCAGGAT AAACCGACAG 
AAGAAAACAA TGAAGTTGTC CCGGGCACTC TCAGACCTGG TGAAATATAC AAAGTCTGTC 
GGCATCCATG ATGTTGAAAC TGAAATCTCT TCCAGCTGGC AGGTTTCATC TTTCAGTGAA 
ACAAAAGCCC ACCAGATACT GCAGCAAAAG CCAGCACAGT ATCTACGTTT CAACCAACAT 
CAGTTATCCC GCATCTACCC ATCTTCTTAC CGTGTAGACT CTAGCAACTA CAATCCTCAG 
CCATTCTGGA ATGCTGGCTG CCAACTTGTG GCTTTAAACT ACCAGTCAGA GGGGAGAATG 
CTACAACTGA ACCGGGCCAA ATTTAGTGCC AATGGGAATT GTGGCTATGT CCTCAAACCA 
AACTGCATGT GTCAAGGCAT CTTTAATCCA AATTCTGAAG ATCCACTGCC TGGTCAGTTG 
AAGAAACAGC TGGTTCTAAG AATTATCAGT GGTCAACAGC TCCCAAAACC ACGGGATTCC 
ATGCTGGGAG ACAGAGGAGA GATCATAGAT CCATTTGTTG AAGTAGAAGT TATAGGCCTA 
CCTGTCGATT GCTTCAAAGA ACAAACTAGA GTAGTTGATG ATAATGGATT TAACCCAATG 
TGGGAAGAAA CATTGGTGTT CACACTGCAC ATGCCAGAGA TTGCATTGAT TCGGTTCCTT 
GTGTGGGATC ATGATCCAAT TGGGCGGGAT TTTATTGGAC AAAGAACGAT AGCTTTCAGC 
AGTATGATGC CAGGTTACCG ACATGTATAC CTAGAAGGTA TAGAAGAGGC ATCCATCTTT 
GTTCATGTGG CTATTAATGA CATTTGTGGT AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC 
CTATTTCTCA GAAATCCAAA GCAGGCCTCC CTGGACAGTC ATGCTGCTGG TCAGCTCCAT 
CGCAAGCATT CCTTTAGCAG CCACATCTTG AGACGGACAG CAAGTGCACC CACCAAAAGC 
CAGAAGAAGA AGAAGGGCTT TCCTGAAATT GTTTTTGACA CAAAAGACAA CAGCTCTGAA 
GGGGCCAGTG AAGACCACGA AGCAGAAGCT GCCTCACAGC CTTGCTTTGA GCAAGAGCCA 
GAGTGTGCTT CTCTGTCACC AGCTCCCAGG GATGGTGCCA GTGGGGAGGT ACATGGCAAA 
GGGTTGAAAG TAGATGTCGG CCGTTTCTTG ATGCAGAGGT CAGTCACATC TTTATGCAGC 
CTAGAAACTA TCACTGAAGA GCCTGTTATT GCAAATGAAA GCCATCATGC CAGCCTCTTC 
TTTCCTCTAC CCGTTACTCC TTACAGTGAT TCTGAAGAAG GCTCTGTGTC TGATCATTTC 
ACTGAATCTC AAACAAAGGG AAGCAACCAA TCGAACCGGC TTCCAGAACC TCCGCTCACT 
AGAGAACAAA GACAAACTTT GGCTGCAGAA GACCAAGCGC AAATAGCCAC AAAAGCAGCT 
GACATTAATG TATTAGTCCA AGATGATGAG AGGACAAGCA TGGCAGAACC TCTCAAAGAA 
GATGATGGTT GGACGCAGGC ATGCAGTGAA GCTGAAGATC AAGTTAATTT GGCAGCGCAT 
CCTCAAAAAG CGTGGCTGGT GGTGGCAGAA GCTAAGAGTA CTGGCCAGCC TGTTGAAAAC 
TGCCAGAAGC AGAATGACCA AACAGGGCAG ATATTGAAAG ACTTGAAAAC CACTGCTGAA 
TTGAAAAGCC AAAAACCTGG TGCAGCAGCT GTCCTGCACC GAAACAATGT GATTAATGGC 
ACACATCCAA CTTTGTCTCC AGATATTTTC CAGAAAGCCC AGGCTGCACA GGCCCTACCT 
GTTTCTCAGA CAAGCTCTTA CATGTTAATA AGGAAGTCAA AGAGTGAAGG GCTGAAAAAG 
TCAGACTCCT CAGCTTTAAT AAAAAATCCA AGTAGCCTAT CTCCAGCAGC AGAAGTATAC 
TTTGATGCTA CTATTAATGA CCGGATCTGG AGCAAGCTAG ATTGCAGCAA TCATCGTGAC 
AGCATGTCTT CCTCTTCCAG CATGTCCTCC AATGACACTG TGATAGATCT GTCTTTACCT 
AATCTAGCTC GCAAAAGCCT TCCAGATCTT GGCATCCGTC ATGAGAACTT TGAGTCCCTT 
GCCATGAGAA AGAGCATTCG CCCACGTTCT GCTACAGCGT CATGCAATGA AATGCCAATA 
GTGAGCAAGA GTAAATCCAA TCCTAACCTG AGGTCTGACC AGCTGCCAGC GGATGAATTA 
TGCCCCCGTC CTCTTGCAAG GAGTCCTCAG GATGCATTCT CATCCATTCC TAGGAGGCAT 
ACCTGGAGCA GGCTGTACAT GGAAAGTCTC AGGCAATCTT CTAACAAATC CAAAGCACAG 
GATAAGGTGG GGAATAACCA GGCAAAATCT AAGAGTCTTG GAGACCTTAC CTCTGATGAC 
ATTGTGTGCA CTTTTGAAAG CAAATATCGT AGCATCAGCA GGAGTTTTGT CACTCGATCA 
ATGCGTGAGC AGCGCCGCTC TGCAGGCCTG AGAACCTCTG TCAAACCCCA GGATGAATTG 
ACTGAGCAGC TGAAGAAGCT AACGGCCTTT CAGCAGGAAA ATGATATAAC TTCCCCCATC 
AGTCTTGATC CAACTGAATC TGAAGAGGAA GCAGAGAGCT TAGGACTCCT TCGCAGGTCA 
TCATCTCGCA GTCAGAGCAG GGTGCGGTAC ATTGCCAACA GAGCCAAGCA AGCTCAGGAG 
AGACAGCGAC TGCAAAGCCT GGGCCAGCTC AGTGGGAGTC CCATTGAAGA GAGAGGAAAT 
CCAGAGGGAG CCTGCAGTGT TGCAAAAGCA GTTTGTATGG ATATAGCATC TCCTGCTGTG 
TTTACATCCC AGCCTAAGAA CCAAACTGAT TATAATACTG ACACAGAAGT CTTCTTTATG 
CTGAAACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142611.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998379.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X8, mRNA.

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CloneID	OGa147101
Clone ID Related Accession (Same CDS sequence)	XM_025142611.1
Accession Version	XM_025142611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAG GAAGCACACC ACAGAAGCAC ATGGAAAGAT GTATGAGCTC CATGCAAGCT 
GGTACCCAAA TGATCAAACT CCGTGGTGGT TCCAAAGGGT TGGTCCGCTT CTATTACCTG 
GATGAGCACA AGTCATGCAT TCGCTGGAGA CCCTCCCGAA AGAATGAGAG AGCCAAAATT 
TCCATCGACT CTATTCAAGA GGTTTGTGAG GGGAAGCAAT CGGAGATCTT TCAGCGCTAT 
GCTGATGGAA GTTTTGATCC TAACTGCTGC TTCAGCATCT ACTATGGAGA TCATATGGAA 
TCCCTTGACC TGGTGTCTCC CAGTGGAGAT GAAGCACGTA CTTGGATCAC AGGGCTAAAA 
TATCTGATGG CAGGGATCAG TGATGAAGAC AGCCTCTCAA AACGCCAAAG AACCAGAGAC 
CAGTGGTTGA AGCAGACATT TGATGAAGCA GATAAAAATG GTGATGGCTG TCTGAGTATT 
ACTGAAGTGC TCCAACTAAT GCACAAACTG AATGTCAACC TACCCCGACA AAAAGTCAAG 
CAAATGTTTA AGGAGGCTGA TACAGACGAT AACCAGGGCA CGCTAGGCTT TGATGAGTTC 
TGCACCTTCT ACAAGATGAT GTCAACACGG CGTGATTTGT ACTTGCTAAT GCTCACCTAC 
AGCAACCACA AGGACTTCCT GGATACAGAT GACCTGAGAC GCTTTCTGGA GAATGAACAA 
AAGATGACAA ATGTGACCAG AGAACACTGC CTGGAAATCA TCAACAAGTT TGAACCATGC 
CTGGAAAACA AGAAGGCGGG AGCTCTGGGG ATTGATGGTT TCACCAATTA CATGCGGAGT 
CCATCAGGGG ACATTTTCAA CCCAGAACAC TACCAAGTGA ATCAGGACAT GAGTTACCCT 
TTGAGTCACT ATTTCATTAC CTCTTCACAT AATACCTACC TCATGGGAGA TCAGCTGATG 
TCTCAGTCTA GGGTGGACAT GTATGCATGG GTGCTACAAT CCGGCTGTCG TTGTGTAGAA 
GTTGACTGCT GGGATGGGCC GGATGGTGAA CCAATTGTCC ACCATGGATA CACTTTGACT 
TCCAAAATTC TCTTCAAAGA TGTGATTGAA ACTATCAACA AATATGCCTT TATCAAGAAT 
GAGTACCCTG TTATCTTGTC AATTGAGAAC CACTGCAGTG TAGTTCAGCA GAAGAAGATG 
GCCCAATATC TTACAGAAAT TCTGGGTGAC AAATTGGATC TGTCTTCTGT GCACAATGAT 
GACTCTACCA GGCTCCCTTC ACCAGCAAGT CTTAAAGGGA AGATTCTGGT TAAGGGGAAG 
AAGCTTCCAG CCAACATTAG TGATGATGCA GAGGAAGGTG AAGTTTCTGA TGAGGACAGT 
GCTGATGAGA TTGATGATGA CTGTAAAATA ATGAATGGAG ATGCATCTAC TAATAGGAAA 
AGAGTGGAGA ATATAGCAAA GAAAAAACTT GACTCACTCA TAAAAGAATC CAAAATTCGG 
GACTGTGAAG ATCCAAACAA TTTTACAGTT ACCACTCTGC CATCATCTGG AAAGGCTGGT 
CAGAAATCAG ACAGTAAGAA GAATAAACTT GAGGATGATG CAGAATCTGG GGAAGACTTC 
AGTACCAATA AAAGGCATAG CCGAACATTA ATGGGCAGCT TTTCTAAACG CAAGAAAAAA 
GGAAGCAAAT TGAAAAAATC AACCAGTCTG GAAGAAGGTG AAGATGATGC TGATTCTCAA 
GGAAATGTAG CCAGAGGCTC TGTACATTAC ACCAGGATAA ACCGACAGAA GAAAACAATG 
AAGTTGTCCC GGGCACTCTC AGACCTGGTG AAATATACAA AGTCTGTCGG CATCCATGAT 
GTTGAAACTG AAATCTCTTC CAGCTGGCAG GTTTCATCTT TCAGTGAAAC AAAAGCCCAC 
CAGATACTGC AGCAAAAGCC AGCACAGTAT CTACGTTTCA ACCAACATCA GTTATCCCGC 
ATCTACCCAT CTTCTTACCG TGTAGACTCT AGCAACTACA ATCCTCAGCC ATTCTGGAAT 
GCTGGCTGCC AACTTGTGGC TTTAAACTAC CAGTCAGAGG GGAGAATGCT ACAACTGAAC 
CGGGCCAAAT TTAGTGCCAA TGGGAATTGT GGCTATGTCC TCAAACCAAA CTGCATGTGT 
CAAGGCATCT TTAATCCAAA TTCTGAAGAT CCACTGCCTG GTCAGTTGAA GAAACAGCTG 
GTTCTAAGAA TTATCAGTGG TCAACAGCTC CCAAAACCAC GGGATTCCAT GCTGGGAGAC 
AGAGGAGAGA TCATAGATCC ATTTGTTGAA GTAGAAGTTA TAGGCCTACC TGTCGATTGC 
TTCAAAGAAC AAACTAGAGT AGTTGATGAT AATGGATTTA ACCCAATGTG GGAAGAAACA 
TTGGTGTTCA CACTGCACAT GCCAGAGATT GCATTGATTC GGTTCCTTGT GTGGGATCAT 
GATCCAATTG GGCGGGATTT TATTGGACAA AGAACGATAG CTTTCAGCAG TATGATGCCA 
GGTTACCGAC ATGTATACCT AGAAGGTATA GAAGAGGCAT CCATCTTTGT TCATGTGGCT 
ATTAATGACA TTTGTGGTAA GGTCAAACAA GCGCTGGGCC TAAAAGGCCT ATTTCTCAGA 
AATCCAAAGC AGGCCTCCCT GGACAGTCAT GCTGCTGGTC AGCTCCATCG CAAGCATTCC 
TTTAGCAGCC ACATCTTGAG ACGGACAGCA AGTGCACCCA CCAAAAGCCA GAAGAAGAAG 
AAGGGCTTTC CTGAAATTGT TTTTGACACA AAAGACAACA GCTCTGAAGG GGCCAGTGAA 
GACCACGAAG CAGAAGCTGC CTCACAGCCT TGCTTTGAGC AAGAGCCAGA GTGTGCTTCT 
CTGTCACCAG CTCCCAGGGA TGGTGCCAGT GGGGAGGTAC ATGGCAAAGG GTTGAAAGTA 
GATGTCGGCC GTTTCTTGAT GCAGAGGTCA GTCACATCTT TATGCAGCCT AGAAACTATC 
ACTGAAGAGC CTGTTATTGC AAATGAAAGC CATCATGCCA GCCTCTTCTT TCCTCTACCC 
GTTACTCCTT ACAGTGATTC TGAAGAAGGC TCTGTGTCTG ATCATTTCAC TGAATCTCAA 
ACAAAGGGAA GCAACCAATC GAACCGGCTT CCAGAACCTC CGCTCACTAG AGAACAAAGA 
CAAACTTTGG CTGCAGAAGA CCAAGCGCAA ATAGCCACAA AAGCAGCTGA CATTAATGTA 
TTAGTCCAAG ATGATGAGAG GACAAGCATG GCAGAACCTC TCAAAGAAGA TGATGGTTGG 
ACGCAGGCAT GCAGTGAAGC TGAAGATCAA GTTAATTTGG CAGCGCATCC TCAAAAAGCG 
TGGCTGGTGG TGGCAGAAGC TAAGAGTACT GGCCAGCCTG TTGAAAACTG CCAGAAGCAG 
AATGACCAAA CAGGGCAGAT ATTGAAAGAC TTGAAAACCA CTGCTGAATT GAAAAGCCAA 
AAACCTGGTG CAGCAGCTGT CCTGCACCGA AACAATGTGA TTAATGGCAC ACATCCAACT 
TTGTCTCCAG ATATTTTCCA GAAAGCCCAG GCTGCACAGG CCCTACCTGT TTCTCAGACA 
AGCTCTTACA TGTTAATAAG GAAGTCAAAG AGTGAAGGGC TGAAAAAGTC AGACTCCTCA 
GCTTTAATAA AAAATCCAAG TAGCCTATCT CCAGCAGCAG AAGTATACTT TGATGCTACT 
ATTAATGACC GGATCTGGAG CAAGCTAGAT TGCAGCAATC ATCGTGACAG CATGTCTTCC 
TCTTCCAGCA TGTCCTCCAA TGACACTGTG ATAGATCTGT CTTTACCTAA TCTAGCTCGC 
AAAAGCCTTC CAGATCTTGG CATCCGTCAT GAGAACTTTG AGTCCCTTGC CATGAGAAAG 
AGCATTCGCC CACGTTCTGC TACAGCGTCA TGCAATGAAA TGCCAATAGT GAGCAAGAGT 
AAATCCAATC CTAACCTGAG GTCTGACCAG CTGCCAGCGG ATGAATTATG CCCCCGTCCT 
CTTGCAAGGA GTCCTCAGGA TGCATTCTCA TCCATTCCTA GGAGGCATAC CTGGAGCAGG 
CTGTACATGG AAAGTCTCAG GCAATCTTCT AACAAATCCA AAGCACAGGA TAAGGTGGGG 
AATAACCAGG CAAAATCTAA GAGTCTTGGA GACCTTACCT CTGATGACAT TGTGTGCACT 
TTTGAAAGCA AATATCGTAG CATCAGCAGG AGTTTTGTCA CTCGATCAAT GCGTGAGCAG 
CGCCGCTCTG CAGGCCTGAG AACCTCTGTC AAACCCCAGG ATGAATTGAC TGAGCAGCTG 
AAGAAGCTAA CGGCCTTTCA GCAGGAAAAT GATATAACTT CCCCCATCAG TCTTGATCCA 
ACTGAATCTG AAGAGGAAGC AGAGAGCTTA GGACTCCTTC GCAGGTCATC ATCTCGCAGT 
CAGAGCAGGG TGCGGTACAT TGCCAACAGA GCCAAGCAAG CTCAGGAGAG ACAGCGACTG 
CAAAGCCTGG GCCAGCTCAG TGGGAGTCCC ATTGAAGAGA GAGGAAATCC AGAGGGAGCC 
TGCAGTGTTG CAAAAGCAGT TTGTATGGAT ATAGCATCTC CTGCTGTGTT TACATCCCAG 
CCTAAGAACC AAACTGATTA TAATACTGAC ACAGAAGTCT TCTTTATGCT GAAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998379.1
CDS	77..4756
Translation	MDLGSTPQKHMERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142611.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142611.1

ORF Insert Sequence:

ATGGATTTAG GAAGCACACC ACAGAAGCAC ATGGAAAGAT GTATGAGCTC CATGCAAGCT 
GGTACCCAAA TGATCAAACT CCGTGGTGGT TCCAAAGGGT TGGTCCGCTT CTATTACCTG 
GATGAGCACA AGTCATGCAT TCGCTGGAGA CCCTCCCGAA AGAATGAGAG AGCCAAAATT 
TCCATCGACT CTATTCAAGA GGTTTGTGAG GGGAAGCAAT CGGAGATCTT TCAGCGCTAT 
GCTGATGGAA GTTTTGATCC TAACTGCTGC TTCAGCATCT ACTATGGAGA TCATATGGAA 
TCCCTTGACC TGGTGTCTCC CAGTGGAGAT GAAGCACGTA CTTGGATCAC AGGGCTAAAA 
TATCTGATGG CAGGGATCAG TGATGAAGAC AGCCTCTCAA AACGCCAAAG AACCAGAGAC 
CAGTGGTTGA AGCAGACATT TGATGAAGCA GATAAAAATG GTGATGGCTG TCTGAGTATT 
ACTGAAGTGC TCCAACTAAT GCACAAACTG AATGTCAACC TACCCCGACA AAAAGTCAAG 
CAAATGTTTA AGGAGGCTGA TACAGACGAT AACCAGGGCA CGCTAGGCTT TGATGAGTTC 
TGCACCTTCT ACAAGATGAT GTCAACACGG CGTGATTTGT ACTTGCTAAT GCTCACCTAC 
AGCAACCACA AGGACTTCCT GGATACAGAT GACCTGAGAC GCTTTCTGGA GAATGAACAA 
AAGATGACAA ATGTGACCAG AGAACACTGC CTGGAAATCA TCAACAAGTT TGAACCATGC 
CTGGAAAACA AGAAGGCGGG AGCTCTGGGG ATTGATGGTT TCACCAATTA CATGCGGAGT 
CCATCAGGGG ACATTTTCAA CCCAGAACAC TACCAAGTGA ATCAGGACAT GAGTTACCCT 
TTGAGTCACT ATTTCATTAC CTCTTCACAT AATACCTACC TCATGGGAGA TCAGCTGATG 
TCTCAGTCTA GGGTGGACAT GTATGCATGG GTGCTACAAT CCGGCTGTCG TTGTGTAGAA 
GTTGACTGCT GGGATGGGCC GGATGGTGAA CCAATTGTCC ACCATGGATA CACTTTGACT 
TCCAAAATTC TCTTCAAAGA TGTGATTGAA ACTATCAACA AATATGCCTT TATCAAGAAT 
GAGTACCCTG TTATCTTGTC AATTGAGAAC CACTGCAGTG TAGTTCAGCA GAAGAAGATG 
GCCCAATATC TTACAGAAAT TCTGGGTGAC AAATTGGATC TGTCTTCTGT GCACAATGAT 
GACTCTACCA GGCTCCCTTC ACCAGCAAGT CTTAAAGGGA AGATTCTGGT TAAGGGGAAG 
AAGCTTCCAG CCAACATTAG TGATGATGCA GAGGAAGGTG AAGTTTCTGA TGAGGACAGT 
GCTGATGAGA TTGATGATGA CTGTAAAATA ATGAATGGAG ATGCATCTAC TAATAGGAAA 
AGAGTGGAGA ATATAGCAAA GAAAAAACTT GACTCACTCA TAAAAGAATC CAAAATTCGG 
GACTGTGAAG ATCCAAACAA TTTTACAGTT ACCACTCTGC CATCATCTGG AAAGGCTGGT 
CAGAAATCAG ACAGTAAGAA GAATAAACTT GAGGATGATG CAGAATCTGG GGAAGACTTC 
AGTACCAATA AAAGGCATAG CCGAACATTA ATGGGCAGCT TTTCTAAACG CAAGAAAAAA 
GGAAGCAAAT TGAAAAAATC AACCAGTCTG GAAGAAGGTG AAGATGATGC TGATTCTCAA 
GGAAATGTAG CCAGAGGCTC TGTACATTAC ACCAGGATAA ACCGACAGAA GAAAACAATG 
AAGTTGTCCC GGGCACTCTC AGACCTGGTG AAATATACAA AGTCTGTCGG CATCCATGAT 
GTTGAAACTG AAATCTCTTC CAGCTGGCAG GTTTCATCTT TCAGTGAAAC AAAAGCCCAC 
CAGATACTGC AGCAAAAGCC AGCACAGTAT CTACGTTTCA ACCAACATCA GTTATCCCGC 
ATCTACCCAT CTTCTTACCG TGTAGACTCT AGCAACTACA ATCCTCAGCC ATTCTGGAAT 
GCTGGCTGCC AACTTGTGGC TTTAAACTAC CAGTCAGAGG GGAGAATGCT ACAACTGAAC 
CGGGCCAAAT TTAGTGCCAA TGGGAATTGT GGCTATGTCC TCAAACCAAA CTGCATGTGT 
CAAGGCATCT TTAATCCAAA TTCTGAAGAT CCACTGCCTG GTCAGTTGAA GAAACAGCTG 
GTTCTAAGAA TTATCAGTGG TCAACAGCTC CCAAAACCAC GGGATTCCAT GCTGGGAGAC 
AGAGGAGAGA TCATAGATCC ATTTGTTGAA GTAGAAGTTA TAGGCCTACC TGTCGATTGC 
TTCAAAGAAC AAACTAGAGT AGTTGATGAT AATGGATTTA ACCCAATGTG GGAAGAAACA 
TTGGTGTTCA CACTGCACAT GCCAGAGATT GCATTGATTC GGTTCCTTGT GTGGGATCAT 
GATCCAATTG GGCGGGATTT TATTGGACAA AGAACGATAG CTTTCAGCAG TATGATGCCA 
GGTTACCGAC ATGTATACCT AGAAGGTATA GAAGAGGCAT CCATCTTTGT TCATGTGGCT 
ATTAATGACA TTTGTGGTAA GGTCAAACAA GCGCTGGGCC TAAAAGGCCT ATTTCTCAGA 
AATCCAAAGC AGGCCTCCCT GGACAGTCAT GCTGCTGGTC AGCTCCATCG CAAGCATTCC 
TTTAGCAGCC ACATCTTGAG ACGGACAGCA AGTGCACCCA CCAAAAGCCA GAAGAAGAAG 
AAGGGCTTTC CTGAAATTGT TTTTGACACA AAAGACAACA GCTCTGAAGG GGCCAGTGAA 
GACCACGAAG CAGAAGCTGC CTCACAGCCT TGCTTTGAGC AAGAGCCAGA GTGTGCTTCT 
CTGTCACCAG CTCCCAGGGA TGGTGCCAGT GGGGAGGTAC ATGGCAAAGG GTTGAAAGTA 
GATGTCGGCC GTTTCTTGAT GCAGAGGTCA GTCACATCTT TATGCAGCCT AGAAACTATC 
ACTGAAGAGC CTGTTATTGC AAATGAAAGC CATCATGCCA GCCTCTTCTT TCCTCTACCC 
GTTACTCCTT ACAGTGATTC TGAAGAAGGC TCTGTGTCTG ATCATTTCAC TGAATCTCAA 
ACAAAGGGAA GCAACCAATC GAACCGGCTT CCAGAACCTC CGCTCACTAG AGAACAAAGA 
CAAACTTTGG CTGCAGAAGA CCAAGCGCAA ATAGCCACAA AAGCAGCTGA CATTAATGTA 
TTAGTCCAAG ATGATGAGAG GACAAGCATG GCAGAACCTC TCAAAGAAGA TGATGGTTGG 
ACGCAGGCAT GCAGTGAAGC TGAAGATCAA GTTAATTTGG CAGCGCATCC TCAAAAAGCG 
TGGCTGGTGG TGGCAGAAGC TAAGAGTACT GGCCAGCCTG TTGAAAACTG CCAGAAGCAG 
AATGACCAAA CAGGGCAGAT ATTGAAAGAC TTGAAAACCA CTGCTGAATT GAAAAGCCAA 
AAACCTGGTG CAGCAGCTGT CCTGCACCGA AACAATGTGA TTAATGGCAC ACATCCAACT 
TTGTCTCCAG ATATTTTCCA GAAAGCCCAG GCTGCACAGG CCCTACCTGT TTCTCAGACA 
AGCTCTTACA TGTTAATAAG GAAGTCAAAG AGTGAAGGGC TGAAAAAGTC AGACTCCTCA 
GCTTTAATAA AAAATCCAAG TAGCCTATCT CCAGCAGCAG AAGTATACTT TGATGCTACT 
ATTAATGACC GGATCTGGAG CAAGCTAGAT TGCAGCAATC ATCGTGACAG CATGTCTTCC 
TCTTCCAGCA TGTCCTCCAA TGACACTGTG ATAGATCTGT CTTTACCTAA TCTAGCTCGC 
AAAAGCCTTC CAGATCTTGG CATCCGTCAT GAGAACTTTG AGTCCCTTGC CATGAGAAAG 
AGCATTCGCC CACGTTCTGC TACAGCGTCA TGCAATGAAA TGCCAATAGT GAGCAAGAGT 
AAATCCAATC CTAACCTGAG GTCTGACCAG CTGCCAGCGG ATGAATTATG CCCCCGTCCT 
CTTGCAAGGA GTCCTCAGGA TGCATTCTCA TCCATTCCTA GGAGGCATAC CTGGAGCAGG 
CTGTACATGG AAAGTCTCAG GCAATCTTCT AACAAATCCA AAGCACAGGA TAAGGTGGGG 
AATAACCAGG CAAAATCTAA GAGTCTTGGA GACCTTACCT CTGATGACAT TGTGTGCACT 
TTTGAAAGCA AATATCGTAG CATCAGCAGG AGTTTTGTCA CTCGATCAAT GCGTGAGCAG 
CGCCGCTCTG CAGGCCTGAG AACCTCTGTC AAACCCCAGG ATGAATTGAC TGAGCAGCTG 
AAGAAGCTAA CGGCCTTTCA GCAGGAAAAT GATATAACTT CCCCCATCAG TCTTGATCCA 
ACTGAATCTG AAGAGGAAGC AGAGAGCTTA GGACTCCTTC GCAGGTCATC ATCTCGCAGT 
CAGAGCAGGG TGCGGTACAT TGCCAACAGA GCCAAGCAAG CTCAGGAGAG ACAGCGACTG 
CAAAGCCTGG GCCAGCTCAG TGGGAGTCCC ATTGAAGAGA GAGGAAATCC AGAGGGAGCC 
TGCAGTGTTG CAAAAGCAGT TTGTATGGAT ATAGCATCTC CTGCTGTGTT TACATCCCAG 
CCTAAGAACC AAACTGATTA TAATACTGAC ACAGAAGTCT TCTTTATGCT GAAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297075.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152561.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X1, mRNA.

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CloneID	OGa45171
Clone ID Related Accession (Same CDS sequence)	XM_015297075.1 , XM_015297078.1 , XM_015297076.1 , XM_015297077.1
Accession Version	XM_015297075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152561.1
CDS	531..6008
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297075.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297075.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297078.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152564.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X4, mRNA.

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CloneID	OGa45171
Clone ID Related Accession (Same CDS sequence)	XM_015297075.1 , XM_015297078.1 , XM_015297076.1 , XM_015297077.1
Accession Version	XM_015297078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152564.1
CDS	468..5945
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297078.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297078.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297076.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152562.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X2, mRNA.

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CloneID	OGa45171
Clone ID Related Accession (Same CDS sequence)	XM_015297075.1 , XM_015297078.1 , XM_015297076.1 , XM_015297077.1
Accession Version	XM_015297076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152562.1
CDS	998..6475
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297076.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297076.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297077.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152563.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X3, mRNA.

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CloneID	OGa45171
Clone ID Related Accession (Same CDS sequence)	XM_015297075.1 , XM_015297078.1 , XM_015297076.1 , XM_015297077.1
Accession Version	XM_015297077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152563.1
CDS	938..6415
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297077.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297077.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGG TAAGGCCCAT GGTTCTCATA AAGCCAAAAG TCAAAGTTGT 
GCTCAAGGGG GTGCTGCTTT CAGTGAACCC ATACACAGGT CTAACAGCGT CCGACTACGG 
GAGCACCAGA GTGAGAAGCA AAGTGTCTTT GCACGCTGCG CCATCAACAG CTCTGGTAGG 
ATTGGAGTGG CCACCAACTG CATGAAATGC ATGATTGGTT CTAAAGAAAG CCCAGATCTA 
GAAAGCAAGT GGAGTGACCA ACCTACCAGG CCTATTGCTA AAGATTTGCT TCACCATGAT 
CAGTATAGCA GTATAACTGG AGAAGAGAGG TTAGAGCTTA AAAACTGGGG TATGAAAGCT 
CAGTCCAGAC CACAGTCAGA TTTGCAGTCT TGCAGTAGCC CTGGAAATGC TGATGATCTA 
ACAAGGAGTA CCCAGTCTTT CGTGACCTCA GGGAAGAAAA ACGTTGAATC TAAATGTCAT 
GGAATAAAAG CTCATTCCCG GCAGCGGTGG CAGTGCCAAT CAGGTGGCAA GTATGGAAGC 
ACTATCAACT TGGTTGCAGC CAGCAATGGC TCCATATCCT CCAACTCCAG CAGTCTAGAA 
AGCCTTGGAA GCCCAGAATT TCCCAAGCGC TGTTCTGAAA CTTCTCGAAG GCAAGTGGGC 
ACCCTACAGA GGGAAATGAA TGCCTTATTC GTTCAAAAAT TGGAGGAAAT TCGAAGTAAA 
TCCCCTATAT TCTTTACTGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297079.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152565.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X5, mRNA.

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CloneID	OGa45172
Clone ID Related Accession (Same CDS sequence)	XM_015297079.1
Accession Version	XM_015297079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATA CAAGCACCAT TAAATGCCTC AGCCCAGATA AAGTGGAAAG ATGTATGAGC 
TCCATGCAAG CTGGTACCCA AATGATCAAA CTCCGTGGTG GTTCCAAAGG GTTGGTCCGC 
TTCTATTACC TGGATGAGCA CAAGTCATGC ATTCGCTGGA GACCCTCCCG AAAGAATGAG 
AGAGCCAAAA TTTCCATCGA CTCTATTCAA GAGGTTTGTG AGGGGAAGCA ATCGGAGATC 
TTTCAGCGCT ATGCTGATGG AAGTTTTGAT CCTAACTGCT GCTTCAGCAT CTACTATGGA 
GATCATATGG AATCCCTTGA CCTGGTGTCT CCCAGTGGAG ATGAAGCACG TACTTGGATC 
ACAGGGCTAA AATATCTGAT GGCAGGGATC AGTGATGAAG ACAGCCTCTC AAAACGCCAA 
AGAACCAGAG ACCAGTGGTT GAAGCAGACA TTTGATGAAG CAGATAAAAA TGGTGATGGC 
TGTCTGAGTA TTACTGAAGT GCTCCAACTA ATGCACAAAC TGAATGTCAA CCTACCCCGA 
CAAAAAGTCA AGCAAATGTT TAAGGAGGCT GATACAGACG ATAACCAGGG CACGCTAGGC 
TTTGATGAGT TCTGCACCTT CTACAAGATG ATGTCAACAC GGCGTGATTT GTACTTGCTA 
ATGCTCACCT ACAGCAACCA CAAGGACTTC CTGGATACAG ATGACCTGAG ACGCTTTCTG 
GAGAATGAAC AAAAGATGAC AAATGTGACC AGAGAACACT GCCTGGAAAT CATCAACAAG 
TTTGAACCAT GCCTGGAAAA CAAGAAGGCG GGAGCTCTGG GGATTGATGG TTTCACCAAT 
TACATGCGGA GTCCATCAGG GGACATTTTC AACCCAGAAC ACTACCAAGT GAATCAGGAC 
ATGAGTTACC CTTTGAGTCA CTATTTCATT ACCTCTTCAC ATAATACCTA CCTCATGGGA 
GATCAGCTGA TGTCTCAGTC TAGGGTGGAC ATGTATGCAT GGGTGCTACA ATCCGGCTGT 
CGTTGTGTAG AAGTTGACTG CTGGGATGGG CCGGATGGTG AACCAATTGT CCACCATGGA 
TACACTTTGA CTTCCAAAAT TCTCTTCAAA GATGTGATTG AAACTATCAA CAAATATGCC 
TTTATCAAGA ATGAGTACCC TGTTATCTTG TCAATTGAGA ACCACTGCAG TGTAGTTCAG 
CAGAAGAAGA TGGCCCAATA TCTTACAGAA ATTCTGGGTG ACAAATTGGA TCTGTCTTCT 
GTGCACAATG ATGACTCTAC CAGGCTCCCT TCACCAGCAA GTCTTAAAGG GAAGATTCTG 
GTTAAGGGGA AGAAGCTTCC AGCCAACATT AGTGATGATG CAGAGGAAGG TGAAGTTTCT 
GATGAGGACA GTGCTGATGA GATTGATGAT GACTGTAAAA TAATGAATGG AGATGCATCT 
ACTAATAGGA AAAGAGTGGA GAATATAGCA AAGAAAAAAC TTGACTCACT CATAAAAGAA 
TCCAAAATTC GGGACTGTGA AGATCCAAAC AATTTTACAG TTACCACTCT GCCATCATCT 
GGAAAGGCTG GTCAGAAATC AGACAGTAAG AAGAATAAAC TTGAGGATGA TGCAGAATCT 
GGGGAAGACT TCAGTACCAA TAAAAGGCAT AGCCGAACAT TAATGGGCAG CTTTTCTAAA 
CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA TCAACCAGTC TGGAAGAAGG TGAAGATGAT 
GCTGATTCTC AAGGAAATGT AGCCAGAGGC TCTGTACATT ACACCAGGAT AAACCGACAG 
AAGAAAACAA TGAAGTTGTC CCGGGCACTC TCAGACCTGG TGAAATATAC AAAGTCTGTC 
GGCATCCATG ATGTTGAAAC TGAAATCTCT TCCAGCTGGC AGGTTTCATC TTTCAGTGAA 
ACAAAAGCCC ACCAGATACT GCAGCAAAAG CCAGCACAGT ATCTACGTTT CAACCAACAT 
CAGTTATCCC GCATCTACCC ATCTTCTTAC CGTGTAGACT CTAGCAACTA CAATCCTCAG 
CCATTCTGGA ATGCTGGCTG CCAACTTGTG GCTTTAAACT ACCAGTCAGA GGGGAGAATG 
CTACAACTGA ACCGGGCCAA ATTTAGTGCC AATGGGAATT GTGGCTATGT CCTCAAACCA 
AACTGCATGT GTCAAGGCAT CTTTAATCCA AATTCTGAAG ATCCACTGCC TGGTCAGTTG 
AAGAAACAGC TGGTTCTAAG AATTATCAGT GGTCAACAGC TCCCAAAACC ACGGGATTCC 
ATGCTGGGAG ACAGAGGAGA GATCATAGAT CCATTTGTTG AAGTAGAAGT TATAGGCCTA 
CCTGTCGATT GCTTCAAAGA ACAAACTAGA GTAGTTGATG ATAATGGATT TAACCCAATG 
TGGGAAGAAA CATTGGTGTT CACACTGCAC ATGCCAGAGA TTGCATTGAT TCGGTTCCTT 
GTGTGGGATC ATGATCCAAT TGGGCGGGAT TTTATTGGAC AAAGAACGAT AGCTTTCAGC 
AGTATGATGC CAGGTTACCG ACATGTATAC CTAGAAGGTA TAGAAGAGGC ATCCATCTTT 
GTTCATGTGG CTATTAATGA CATTTGTGGT AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC 
CTATTTCTCA GAAATCCAAA GCAGGCCTCC CTGGACAGTC ATGCTGCTGG TCAGCTCCAT 
CGCAAGCATT CCTTTAGCAG CCACATCTTG AGACGGACAG CAAGTGCACC CACCAAAAGC 
CAGAAGAAGA AGAAGGGCTT TCCTGAAATT GTTTTTGACA CAAAAGACAA CAGCTCTGAA 
GGGGCCAGTG AAGACCACGA AGCAGAAGCT GCCTCACAGC CTTGCTTTGA GCAAGAGCCA 
GAGTGTGCTT CTCTGTCACC AGCTCCCAGG GATGGTGCCA GTGGGGAGGT ACATGGCAAA 
GGGTTGAAAG GTAAGGCCCA TGGTTCTCAT AAAGCCAAAA GTCAAAGTTG TGCTCAAGGG 
GGTGCTGCTT TCAGTGAACC CATACACAGG TCTAACAGCG TCCGACTACG GGAGCACCAG 
AGTGAGAAGC AAAGTGTCTT TGCACGCTGC GCCATCAACA GCTCTGGTAG GATTGGAGTG 
GCCACCAACT GCATGAAATG CATGATTGGT TCTAAAGAAA GCCCAGATCT AGAAAGCAAG 
TGGAGTGACC AACCTACCAG GCCTATTGCT AAAGATTTGC TTCACCATGA TCAGTATAGC 
AGTATAACTG GAGAAGAGAG GTTAGAGCTT AAAAACTGGG GTATGAAAGC TCAGTCCAGA 
CCACAGTCAG ATTTGCAGTC TTGCAGTAGC CCTGGAAATG CTGATGATCT AACAAGGAGT 
ACCCAGTCTT TCGTGACCTC AGGGAAGAAA AACGTTGAAT CTAAATGTCA TGGAATAAAA 
GCTCATTCCC GGCAGCGGTG GCAGTGCCAA TCAGGTGGCA AGTATGGAAG CACTATCAAC 
TTGGTTGCAG CCAGCAATGG CTCCATATCC TCCAACTCCA GCAGTCTAGA AAGCCTTGGA 
AGCCCAGAAT TTCCCAAGCG CTGTTCTGAA ACTTCTCGAA GGCAAGTGGG CACCCTACAG 
AGGGAAATGA ATGCCTTATT CGTTCAAAAA TTGGAGGAAA TTCGAAGTAA ATCCCCTATA 
TTCTTTACTG ATGTCGGCCG TTTCTTGATG CAGAGGTCAG TCACATCTTT ATGCAGCCTA 
GAAACTATCA CTGAAGAGCC TGTTATTGCA AATGAAAGCC ATCATGCCAG CCTCTTCTTT 
CCTCTACCCG TTACTCCTTA CAGTGATTCT GAAGAAGGCT CTGTGTCTGA TCATTTCACT 
GAATCTCAAA CAAAGGGAAG CAACCAATCG AACCGGCTTC CAGAACCTCC GCTCACTAGA 
GAACAAAGAC AAACTTTGGC TGCAGAAGAC CAAGCGCAAA TAGCCACAAA AGCAGCTGAC 
ATTAATGTAT TAGTCCAAGA TGATGAGAGG ACAAGCATGG CAGAACCTCT CAAAGAAGAT 
GATGGTTGGA CGCAGGCATG CAGTGAAGCT GAAGATCAAG TTAATTTGGC AGCGCATCCT 
CAAAAAGCGT GGCTGGTGGT GGCAGAAGCT AAGAGTACTG GCCAGCCTGT TGAAAACTGC 
CAGAAGCAGA ATGACCAAAC AGGGCAGATA TTGAAAGACT TGAAAACCAC TGCTGAATTG 
AAAAGCCAAA AACCTGGTGC AGCAGCTGTC CTGCACCGAA ACAATGTGAT TAATGGCACA 
CATCCAACTT TGTCTCCAGA TATTTTCCAG AAAGCCCAGG CTGCACAGGC CCTACCTGTT 
TCTCAGACAA GCTCTTACAT GTTAATAAGG AAGTCAAAGA GTGAAGGGCT GAAAAAGTCA 
GACTCCTCAG CTTTAATAAA AAATCCAAGT AGCCTATCTC CAGCAGCAGA AGTATACTTT 
GATGCTACTA TTAATGACCG GATCTGGAGC AAGCTAGATT GCAGCAATCA TCGTGACAGC 
ATGTCTTCCT CTTCCAGCAT GTCCTCCAAT GACACTGTGA TAGATCTGTC TTTACCTAAT 
CTAGCTCGCA AAAGCCTTCC AGATCTTGGC ATCCGTCATG AGAACTTTGA GTCCCTTGCC 
ATGAGAAAGA GCATTCGCCC ACGTTCTGCT ACAGCGTCAT GCAATGAAAT GCCAATAGTG 
AGCAAGAGTA AATCCAATCC TAACCTGAGG TCTGACCAGC TGCCAGCGGA TGAATTATGC 
CCCCGTCCTC TTGCAAGGAG TCCTCAGGAT GCATTCTCAT CCATTCCTAG GAGGCATACC 
TGGAGCAGGC TGTACATGGA AAGTCTCAGG CAATCTTCTA ACAAATCCAA AGCACAGGAT 
AAGGTGGGGA ATAACCAGGC AAAATCTAAG AGTCTTGGAG ACCTTACCTC TGATGACATT 
GTGTGCACTT TTGAAAGCAA ATATCGTAGC ATCAGCAGGA GTTTTGTCAC TCGATCAATG 
CGTGAGCAGC GCCGCTCTGC AGGCCTGAGA ACCTCTGTCA AACCCCAGGA TGAATTGACT 
GAGCAGCTGA AGAAGCTAAC GGCCTTTCAG CAGGAAAATG ATATAACTTC CCCCATCAGT 
CTTGATCCAA CTGAATCTGA AGAGGAAGCA GAGAGCTTAG GACTCCTTCG CAGGTCATCA 
TCTCGCAGTC AGAGCAGGGT GCGGTACATT GCCAACAGAG CCAAGCAAGC TCAGGAGAGA 
CAGCGACTGC AAAGCCTGGG CCAGCTCAGT GGGAGTCCCA TTGAAGAGAG AGGAAATCCA 
GAGGGAGCCT GCAGTGTTGC AAAAGCAGTT TGTATGGATA TAGCATCTCC TGCTGTGTTT 
ACATCCCAGC CTAAGAACCA AACTGATTAT AATACTGACA CAGAAGTCTT CTTTATGCTG 
AAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152565.1
CDS	682..6090
Translation	MDDTSTIKCLSPDKVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297079.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297079.1

ORF Insert Sequence:

ATGGATGATA CAAGCACCAT TAAATGCCTC AGCCCAGATA AAGTGGAAAG ATGTATGAGC 
TCCATGCAAG CTGGTACCCA AATGATCAAA CTCCGTGGTG GTTCCAAAGG GTTGGTCCGC 
TTCTATTACC TGGATGAGCA CAAGTCATGC ATTCGCTGGA GACCCTCCCG AAAGAATGAG 
AGAGCCAAAA TTTCCATCGA CTCTATTCAA GAGGTTTGTG AGGGGAAGCA ATCGGAGATC 
TTTCAGCGCT ATGCTGATGG AAGTTTTGAT CCTAACTGCT GCTTCAGCAT CTACTATGGA 
GATCATATGG AATCCCTTGA CCTGGTGTCT CCCAGTGGAG ATGAAGCACG TACTTGGATC 
ACAGGGCTAA AATATCTGAT GGCAGGGATC AGTGATGAAG ACAGCCTCTC AAAACGCCAA 
AGAACCAGAG ACCAGTGGTT GAAGCAGACA TTTGATGAAG CAGATAAAAA TGGTGATGGC 
TGTCTGAGTA TTACTGAAGT GCTCCAACTA ATGCACAAAC TGAATGTCAA CCTACCCCGA 
CAAAAAGTCA AGCAAATGTT TAAGGAGGCT GATACAGACG ATAACCAGGG CACGCTAGGC 
TTTGATGAGT TCTGCACCTT CTACAAGATG ATGTCAACAC GGCGTGATTT GTACTTGCTA 
ATGCTCACCT ACAGCAACCA CAAGGACTTC CTGGATACAG ATGACCTGAG ACGCTTTCTG 
GAGAATGAAC AAAAGATGAC AAATGTGACC AGAGAACACT GCCTGGAAAT CATCAACAAG 
TTTGAACCAT GCCTGGAAAA CAAGAAGGCG GGAGCTCTGG GGATTGATGG TTTCACCAAT 
TACATGCGGA GTCCATCAGG GGACATTTTC AACCCAGAAC ACTACCAAGT GAATCAGGAC 
ATGAGTTACC CTTTGAGTCA CTATTTCATT ACCTCTTCAC ATAATACCTA CCTCATGGGA 
GATCAGCTGA TGTCTCAGTC TAGGGTGGAC ATGTATGCAT GGGTGCTACA ATCCGGCTGT 
CGTTGTGTAG AAGTTGACTG CTGGGATGGG CCGGATGGTG AACCAATTGT CCACCATGGA 
TACACTTTGA CTTCCAAAAT TCTCTTCAAA GATGTGATTG AAACTATCAA CAAATATGCC 
TTTATCAAGA ATGAGTACCC TGTTATCTTG TCAATTGAGA ACCACTGCAG TGTAGTTCAG 
CAGAAGAAGA TGGCCCAATA TCTTACAGAA ATTCTGGGTG ACAAATTGGA TCTGTCTTCT 
GTGCACAATG ATGACTCTAC CAGGCTCCCT TCACCAGCAA GTCTTAAAGG GAAGATTCTG 
GTTAAGGGGA AGAAGCTTCC AGCCAACATT AGTGATGATG CAGAGGAAGG TGAAGTTTCT 
GATGAGGACA GTGCTGATGA GATTGATGAT GACTGTAAAA TAATGAATGG AGATGCATCT 
ACTAATAGGA AAAGAGTGGA GAATATAGCA AAGAAAAAAC TTGACTCACT CATAAAAGAA 
TCCAAAATTC GGGACTGTGA AGATCCAAAC AATTTTACAG TTACCACTCT GCCATCATCT 
GGAAAGGCTG GTCAGAAATC AGACAGTAAG AAGAATAAAC TTGAGGATGA TGCAGAATCT 
GGGGAAGACT TCAGTACCAA TAAAAGGCAT AGCCGAACAT TAATGGGCAG CTTTTCTAAA 
CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA TCAACCAGTC TGGAAGAAGG TGAAGATGAT 
GCTGATTCTC AAGGAAATGT AGCCAGAGGC TCTGTACATT ACACCAGGAT AAACCGACAG 
AAGAAAACAA TGAAGTTGTC CCGGGCACTC TCAGACCTGG TGAAATATAC AAAGTCTGTC 
GGCATCCATG ATGTTGAAAC TGAAATCTCT TCCAGCTGGC AGGTTTCATC TTTCAGTGAA 
ACAAAAGCCC ACCAGATACT GCAGCAAAAG CCAGCACAGT ATCTACGTTT CAACCAACAT 
CAGTTATCCC GCATCTACCC ATCTTCTTAC CGTGTAGACT CTAGCAACTA CAATCCTCAG 
CCATTCTGGA ATGCTGGCTG CCAACTTGTG GCTTTAAACT ACCAGTCAGA GGGGAGAATG 
CTACAACTGA ACCGGGCCAA ATTTAGTGCC AATGGGAATT GTGGCTATGT CCTCAAACCA 
AACTGCATGT GTCAAGGCAT CTTTAATCCA AATTCTGAAG ATCCACTGCC TGGTCAGTTG 
AAGAAACAGC TGGTTCTAAG AATTATCAGT GGTCAACAGC TCCCAAAACC ACGGGATTCC 
ATGCTGGGAG ACAGAGGAGA GATCATAGAT CCATTTGTTG AAGTAGAAGT TATAGGCCTA 
CCTGTCGATT GCTTCAAAGA ACAAACTAGA GTAGTTGATG ATAATGGATT TAACCCAATG 
TGGGAAGAAA CATTGGTGTT CACACTGCAC ATGCCAGAGA TTGCATTGAT TCGGTTCCTT 
GTGTGGGATC ATGATCCAAT TGGGCGGGAT TTTATTGGAC AAAGAACGAT AGCTTTCAGC 
AGTATGATGC CAGGTTACCG ACATGTATAC CTAGAAGGTA TAGAAGAGGC ATCCATCTTT 
GTTCATGTGG CTATTAATGA CATTTGTGGT AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC 
CTATTTCTCA GAAATCCAAA GCAGGCCTCC CTGGACAGTC ATGCTGCTGG TCAGCTCCAT 
CGCAAGCATT CCTTTAGCAG CCACATCTTG AGACGGACAG CAAGTGCACC CACCAAAAGC 
CAGAAGAAGA AGAAGGGCTT TCCTGAAATT GTTTTTGACA CAAAAGACAA CAGCTCTGAA 
GGGGCCAGTG AAGACCACGA AGCAGAAGCT GCCTCACAGC CTTGCTTTGA GCAAGAGCCA 
GAGTGTGCTT CTCTGTCACC AGCTCCCAGG GATGGTGCCA GTGGGGAGGT ACATGGCAAA 
GGGTTGAAAG GTAAGGCCCA TGGTTCTCAT AAAGCCAAAA GTCAAAGTTG TGCTCAAGGG 
GGTGCTGCTT TCAGTGAACC CATACACAGG TCTAACAGCG TCCGACTACG GGAGCACCAG 
AGTGAGAAGC AAAGTGTCTT TGCACGCTGC GCCATCAACA GCTCTGGTAG GATTGGAGTG 
GCCACCAACT GCATGAAATG CATGATTGGT TCTAAAGAAA GCCCAGATCT AGAAAGCAAG 
TGGAGTGACC AACCTACCAG GCCTATTGCT AAAGATTTGC TTCACCATGA TCAGTATAGC 
AGTATAACTG GAGAAGAGAG GTTAGAGCTT AAAAACTGGG GTATGAAAGC TCAGTCCAGA 
CCACAGTCAG ATTTGCAGTC TTGCAGTAGC CCTGGAAATG CTGATGATCT AACAAGGAGT 
ACCCAGTCTT TCGTGACCTC AGGGAAGAAA AACGTTGAAT CTAAATGTCA TGGAATAAAA 
GCTCATTCCC GGCAGCGGTG GCAGTGCCAA TCAGGTGGCA AGTATGGAAG CACTATCAAC 
TTGGTTGCAG CCAGCAATGG CTCCATATCC TCCAACTCCA GCAGTCTAGA AAGCCTTGGA 
AGCCCAGAAT TTCCCAAGCG CTGTTCTGAA ACTTCTCGAA GGCAAGTGGG CACCCTACAG 
AGGGAAATGA ATGCCTTATT CGTTCAAAAA TTGGAGGAAA TTCGAAGTAA ATCCCCTATA 
TTCTTTACTG ATGTCGGCCG TTTCTTGATG CAGAGGTCAG TCACATCTTT ATGCAGCCTA 
GAAACTATCA CTGAAGAGCC TGTTATTGCA AATGAAAGCC ATCATGCCAG CCTCTTCTTT 
CCTCTACCCG TTACTCCTTA CAGTGATTCT GAAGAAGGCT CTGTGTCTGA TCATTTCACT 
GAATCTCAAA CAAAGGGAAG CAACCAATCG AACCGGCTTC CAGAACCTCC GCTCACTAGA 
GAACAAAGAC AAACTTTGGC TGCAGAAGAC CAAGCGCAAA TAGCCACAAA AGCAGCTGAC 
ATTAATGTAT TAGTCCAAGA TGATGAGAGG ACAAGCATGG CAGAACCTCT CAAAGAAGAT 
GATGGTTGGA CGCAGGCATG CAGTGAAGCT GAAGATCAAG TTAATTTGGC AGCGCATCCT 
CAAAAAGCGT GGCTGGTGGT GGCAGAAGCT AAGAGTACTG GCCAGCCTGT TGAAAACTGC 
CAGAAGCAGA ATGACCAAAC AGGGCAGATA TTGAAAGACT TGAAAACCAC TGCTGAATTG 
AAAAGCCAAA AACCTGGTGC AGCAGCTGTC CTGCACCGAA ACAATGTGAT TAATGGCACA 
CATCCAACTT TGTCTCCAGA TATTTTCCAG AAAGCCCAGG CTGCACAGGC CCTACCTGTT 
TCTCAGACAA GCTCTTACAT GTTAATAAGG AAGTCAAAGA GTGAAGGGCT GAAAAAGTCA 
GACTCCTCAG CTTTAATAAA AAATCCAAGT AGCCTATCTC CAGCAGCAGA AGTATACTTT 
GATGCTACTA TTAATGACCG GATCTGGAGC AAGCTAGATT GCAGCAATCA TCGTGACAGC 
ATGTCTTCCT CTTCCAGCAT GTCCTCCAAT GACACTGTGA TAGATCTGTC TTTACCTAAT 
CTAGCTCGCA AAAGCCTTCC AGATCTTGGC ATCCGTCATG AGAACTTTGA GTCCCTTGCC 
ATGAGAAAGA GCATTCGCCC ACGTTCTGCT ACAGCGTCAT GCAATGAAAT GCCAATAGTG 
AGCAAGAGTA AATCCAATCC TAACCTGAGG TCTGACCAGC TGCCAGCGGA TGAATTATGC 
CCCCGTCCTC TTGCAAGGAG TCCTCAGGAT GCATTCTCAT CCATTCCTAG GAGGCATACC 
TGGAGCAGGC TGTACATGGA AAGTCTCAGG CAATCTTCTA ACAAATCCAA AGCACAGGAT 
AAGGTGGGGA ATAACCAGGC AAAATCTAAG AGTCTTGGAG ACCTTACCTC TGATGACATT 
GTGTGCACTT TTGAAAGCAA ATATCGTAGC ATCAGCAGGA GTTTTGTCAC TCGATCAATG 
CGTGAGCAGC GCCGCTCTGC AGGCCTGAGA ACCTCTGTCA AACCCCAGGA TGAATTGACT 
GAGCAGCTGA AGAAGCTAAC GGCCTTTCAG CAGGAAAATG ATATAACTTC CCCCATCAGT 
CTTGATCCAA CTGAATCTGA AGAGGAAGCA GAGAGCTTAG GACTCCTTCG CAGGTCATCA 
TCTCGCAGTC AGAGCAGGGT GCGGTACATT GCCAACAGAG CCAAGCAAGC TCAGGAGAGA 
CAGCGACTGC AAAGCCTGGG CCAGCTCAGT GGGAGTCCCA TTGAAGAGAG AGGAAATCCA 
GAGGGAGCCT GCAGTGTTGC AAAAGCAGTT TGTATGGATA TAGCATCTCC TGCTGTGTTT 
ACATCCCAGC CTAAGAACCA AACTGATTAT AATACTGACA CAGAAGTCTT CTTTATGCTG 
AAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297080.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152566.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X6, mRNA.

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CloneID	OGa45173
Clone ID Related Accession (Same CDS sequence)	XM_015297080.1
Accession Version	XM_015297080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAG GAAGCACACC ACAGAAGCAC ATGGAAAGAT GTATGAGCTC CATGCAAGCT 
GGTACCCAAA TGATCAAACT CCGTGGTGGT TCCAAAGGGT TGGTCCGCTT CTATTACCTG 
GATGAGCACA AGTCATGCAT TCGCTGGAGA CCCTCCCGAA AGAATGAGAG AGCCAAAATT 
TCCATCGACT CTATTCAAGA GGTTTGTGAG GGGAAGCAAT CGGAGATCTT TCAGCGCTAT 
GCTGATGGAA GTTTTGATCC TAACTGCTGC TTCAGCATCT ACTATGGAGA TCATATGGAA 
TCCCTTGACC TGGTGTCTCC CAGTGGAGAT GAAGCACGTA CTTGGATCAC AGGGCTAAAA 
TATCTGATGG CAGGGATCAG TGATGAAGAC AGCCTCTCAA AACGCCAAAG AACCAGAGAC 
CAGTGGTTGA AGCAGACATT TGATGAAGCA GATAAAAATG GTGATGGCTG TCTGAGTATT 
ACTGAAGTGC TCCAACTAAT GCACAAACTG AATGTCAACC TACCCCGACA AAAAGTCAAG 
CAAATGTTTA AGGAGGCTGA TACAGACGAT AACCAGGGCA CGCTAGGCTT TGATGAGTTC 
TGCACCTTCT ACAAGATGAT GTCAACACGG CGTGATTTGT ACTTGCTAAT GCTCACCTAC 
AGCAACCACA AGGACTTCCT GGATACAGAT GACCTGAGAC GCTTTCTGGA GAATGAACAA 
AAGATGACAA ATGTGACCAG AGAACACTGC CTGGAAATCA TCAACAAGTT TGAACCATGC 
CTGGAAAACA AGAAGGCGGG AGCTCTGGGG ATTGATGGTT TCACCAATTA CATGCGGAGT 
CCATCAGGGG ACATTTTCAA CCCAGAACAC TACCAAGTGA ATCAGGACAT GAGTTACCCT 
TTGAGTCACT ATTTCATTAC CTCTTCACAT AATACCTACC TCATGGGAGA TCAGCTGATG 
TCTCAGTCTA GGGTGGACAT GTATGCATGG GTGCTACAAT CCGGCTGTCG TTGTGTAGAA 
GTTGACTGCT GGGATGGGCC GGATGGTGAA CCAATTGTCC ACCATGGATA CACTTTGACT 
TCCAAAATTC TCTTCAAAGA TGTGATTGAA ACTATCAACA AATATGCCTT TATCAAGAAT 
GAGTACCCTG TTATCTTGTC AATTGAGAAC CACTGCAGTG TAGTTCAGCA GAAGAAGATG 
GCCCAATATC TTACAGAAAT TCTGGGTGAC AAATTGGATC TGTCTTCTGT GCACAATGAT 
GACTCTACCA GGCTCCCTTC ACCAGCAAGT CTTAAAGGGA AGATTCTGGT TAAGGGGAAG 
AAGCTTCCAG CCAACATTAG TGATGATGCA GAGGAAGGTG AAGTTTCTGA TGAGGACAGT 
GCTGATGAGA TTGATGATGA CTGTAAAATA ATGAATGGAG ATGCATCTAC TAATAGGAAA 
AGAGTGGAGA ATATAGCAAA GAAAAAACTT GACTCACTCA TAAAAGAATC CAAAATTCGG 
GACTGTGAAG ATCCAAACAA TTTTACAGTT ACCACTCTGC CATCATCTGG AAAGGCTGGT 
CAGAAATCAG ACAGTAAGAA GAATAAACTT GAGGATGATG CAGAATCTGG GGAAGACTTC 
AGTACCAATA AAAGGCATAG CCGAACATTA ATGGGCAGCT TTTCTAAACG CAAGAAAAAA 
GGAAGCAAAT TGAAAAAATC AACCAGTCTG GAAGAAGGTG AAGATGATGC TGATTCTCAA 
GGAAATGTAG CCAGAGGCTC TGTACATTAC ACCAGGATAA ACCGACAGAA GAAAACAATG 
AAGTTGTCCC GGGCACTCTC AGACCTGGTG AAATATACAA AGTCTGTCGG CATCCATGAT 
GTTGAAACTG AAATCTCTTC CAGCTGGCAG GTTTCATCTT TCAGTGAAAC AAAAGCCCAC 
CAGATACTGC AGCAAAAGCC AGCACAGTAT CTACGTTTCA ACCAACATCA GTTATCCCGC 
ATCTACCCAT CTTCTTACCG TGTAGACTCT AGCAACTACA ATCCTCAGCC ATTCTGGAAT 
GCTGGCTGCC AACTTGTGGC TTTAAACTAC CAGTCAGAGG GGAGAATGCT ACAACTGAAC 
CGGGCCAAAT TTAGTGCCAA TGGGAATTGT GGCTATGTCC TCAAACCAAA CTGCATGTGT 
CAAGGCATCT TTAATCCAAA TTCTGAAGAT CCACTGCCTG GTCAGTTGAA GAAACAGCTG 
GTTCTAAGAA TTATCAGTGG TCAACAGCTC CCAAAACCAC GGGATTCCAT GCTGGGAGAC 
AGAGGAGAGA TCATAGATCC ATTTGTTGAA GTAGAAGTTA TAGGCCTACC TGTCGATTGC 
TTCAAAGAAC AAACTAGAGT AGTTGATGAT AATGGATTTA ACCCAATGTG GGAAGAAACA 
TTGGTGTTCA CACTGCACAT GCCAGAGATT GCATTGATTC GGTTCCTTGT GTGGGATCAT 
GATCCAATTG GGCGGGATTT TATTGGACAA AGAACGATAG CTTTCAGCAG TATGATGCCA 
GGTTACCGAC ATGTATACCT AGAAGGTATA GAAGAGGCAT CCATCTTTGT TCATGTGGCT 
ATTAATGACA TTTGTGGTAA GGTCAAACAA GCGCTGGGCC TAAAAGGCCT ATTTCTCAGA 
AATCCAAAGC AGGCCTCCCT GGACAGTCAT GCTGCTGGTC AGCTCCATCG CAAGCATTCC 
TTTAGCAGCC ACATCTTGAG ACGGACAGCA AGTGCACCCA CCAAAAGCCA GAAGAAGAAG 
AAGGGCTTTC CTGAAATTGT TTTTGACACA AAAGACAACA GCTCTGAAGG GGCCAGTGAA 
GACCACGAAG CAGAAGCTGC CTCACAGCCT TGCTTTGAGC AAGAGCCAGA GTGTGCTTCT 
CTGTCACCAG CTCCCAGGGA TGGTGCCAGT GGGGAGGTAC ATGGCAAAGG GTTGAAAGGT 
AAGGCCCATG GTTCTCATAA AGCCAAAAGT CAAAGTTGTG CTCAAGGGGG TGCTGCTTTC 
AGTGAACCCA TACACAGGTC TAACAGCGTC CGACTACGGG AGCACCAGAG TGAGAAGCAA 
AGTGTCTTTG CACGCTGCGC CATCAACAGC TCTGGTAGGA TTGGAGTGGC CACCAACTGC 
ATGAAATGCA TGATTGGTTC TAAAGAAAGC CCAGATCTAG AAAGCAAGTG GAGTGACCAA 
CCTACCAGGC CTATTGCTAA AGATTTGCTT CACCATGATC AGTATAGCAG TATAACTGGA 
GAAGAGAGGT TAGAGCTTAA AAACTGGGGT ATGAAAGCTC AGTCCAGACC ACAGTCAGAT 
TTGCAGTCTT GCAGTAGCCC TGGAAATGCT GATGATCTAA CAAGGAGTAC CCAGTCTTTC 
GTGACCTCAG GGAAGAAAAA CGTTGAATCT AAATGTCATG GAATAAAAGC TCATTCCCGG 
CAGCGGTGGC AGTGCCAATC AGGTGGCAAG TATGGAAGCA CTATCAACTT GGTTGCAGCC 
AGCAATGGCT CCATATCCTC CAACTCCAGC AGTCTAGAAA GCCTTGGAAG CCCAGAATTT 
CCCAAGCGCT GTTCTGAAAC TTCTCGAAGG CAAGTGGGCA CCCTACAGAG GGAAATGAAT 
GCCTTATTCG TTCAAAAATT GGAGGAAATT CGAAGTAAAT CCCCTATATT CTTTACTGAT 
GTCGGCCGTT TCTTGATGCA GAGGTCAGTC ACATCTTTAT GCAGCCTAGA AACTATCACT 
GAAGAGCCTG TTATTGCAAA TGAAAGCCAT CATGCCAGCC TCTTCTTTCC TCTACCCGTT 
ACTCCTTACA GTGATTCTGA AGAAGGCTCT GTGTCTGATC ATTTCACTGA ATCTCAAACA 
AAGGGAAGCA ACCAATCGAA CCGGCTTCCA GAACCTCCGC TCACTAGAGA ACAAAGACAA 
ACTTTGGCTG CAGAAGACCA AGCGCAAATA GCCACAAAAG CAGCTGACAT TAATGTATTA 
GTCCAAGATG ATGAGAGGAC AAGCATGGCA GAACCTCTCA AAGAAGATGA TGGTTGGACG 
CAGGCATGCA GTGAAGCTGA AGATCAAGTT AATTTGGCAG CGCATCCTCA AAAAGCGTGG 
CTGGTGGTGG CAGAAGCTAA GAGTACTGGC CAGCCTGTTG AAAACTGCCA GAAGCAGAAT 
GACCAAACAG GGCAGATATT GAAAGACTTG AAAACCACTG CTGAATTGAA AAGCCAAAAA 
CCTGGTGCAG CAGCTGTCCT GCACCGAAAC AATGTGATTA ATGGCACACA TCCAACTTTG 
TCTCCAGATA TTTTCCAGAA AGCCCAGGCT GCACAGGCCC TACCTGTTTC TCAGACAAGC 
TCTTACATGT TAATAAGGAA GTCAAAGAGT GAAGGGCTGA AAAAGTCAGA CTCCTCAGCT 
TTAATAAAAA ATCCAAGTAG CCTATCTCCA GCAGCAGAAG TATACTTTGA TGCTACTATT 
AATGACCGGA TCTGGAGCAA GCTAGATTGC AGCAATCATC GTGACAGCAT GTCTTCCTCT 
TCCAGCATGT CCTCCAATGA CACTGTGATA GATCTGTCTT TACCTAATCT AGCTCGCAAA 
AGCCTTCCAG ATCTTGGCAT CCGTCATGAG AACTTTGAGT CCCTTGCCAT GAGAAAGAGC 
ATTCGCCCAC GTTCTGCTAC AGCGTCATGC AATGAAATGC CAATAGTGAG CAAGAGTAAA 
TCCAATCCTA ACCTGAGGTC TGACCAGCTG CCAGCGGATG AATTATGCCC CCGTCCTCTT 
GCAAGGAGTC CTCAGGATGC ATTCTCATCC ATTCCTAGGA GGCATACCTG GAGCAGGCTG 
TACATGGAAA GTCTCAGGCA ATCTTCTAAC AAATCCAAAG CACAGGATAA GGTGGGGAAT 
AACCAGGCAA AATCTAAGAG TCTTGGAGAC CTTACCTCTG ATGACATTGT GTGCACTTTT 
GAAAGCAAAT ATCGTAGCAT CAGCAGGAGT TTTGTCACTC GATCAATGCG TGAGCAGCGC 
CGCTCTGCAG GCCTGAGAAC CTCTGTCAAA CCCCAGGATG AATTGACTGA GCAGCTGAAG 
AAGCTAACGG CCTTTCAGCA GGAAAATGAT ATAACTTCCC CCATCAGTCT TGATCCAACT 
GAATCTGAAG AGGAAGCAGA GAGCTTAGGA CTCCTTCGCA GGTCATCATC TCGCAGTCAG 
AGCAGGGTGC GGTACATTGC CAACAGAGCC AAGCAAGCTC AGGAGAGACA GCGACTGCAA 
AGCCTGGGCC AGCTCAGTGG GAGTCCCATT GAAGAGAGAG GAAATCCAGA GGGAGCCTGC 
AGTGTTGCAA AAGCAGTTTG TATGGATATA GCATCTCCTG CTGTGTTTAC ATCCCAGCCT 
AAGAACCAAA CTGATTATAA TACTGACACA GAAGTCTTCT TTATGCTGAA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152566.1
CDS	151..5547
Translation	MDLGSTPQKHMERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297080.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297080.1

ORF Insert Sequence:

ATGGATTTAG GAAGCACACC ACAGAAGCAC ATGGAAAGAT GTATGAGCTC CATGCAAGCT 
GGTACCCAAA TGATCAAACT CCGTGGTGGT TCCAAAGGGT TGGTCCGCTT CTATTACCTG 
GATGAGCACA AGTCATGCAT TCGCTGGAGA CCCTCCCGAA AGAATGAGAG AGCCAAAATT 
TCCATCGACT CTATTCAAGA GGTTTGTGAG GGGAAGCAAT CGGAGATCTT TCAGCGCTAT 
GCTGATGGAA GTTTTGATCC TAACTGCTGC TTCAGCATCT ACTATGGAGA TCATATGGAA 
TCCCTTGACC TGGTGTCTCC CAGTGGAGAT GAAGCACGTA CTTGGATCAC AGGGCTAAAA 
TATCTGATGG CAGGGATCAG TGATGAAGAC AGCCTCTCAA AACGCCAAAG AACCAGAGAC 
CAGTGGTTGA AGCAGACATT TGATGAAGCA GATAAAAATG GTGATGGCTG TCTGAGTATT 
ACTGAAGTGC TCCAACTAAT GCACAAACTG AATGTCAACC TACCCCGACA AAAAGTCAAG 
CAAATGTTTA AGGAGGCTGA TACAGACGAT AACCAGGGCA CGCTAGGCTT TGATGAGTTC 
TGCACCTTCT ACAAGATGAT GTCAACACGG CGTGATTTGT ACTTGCTAAT GCTCACCTAC 
AGCAACCACA AGGACTTCCT GGATACAGAT GACCTGAGAC GCTTTCTGGA GAATGAACAA 
AAGATGACAA ATGTGACCAG AGAACACTGC CTGGAAATCA TCAACAAGTT TGAACCATGC 
CTGGAAAACA AGAAGGCGGG AGCTCTGGGG ATTGATGGTT TCACCAATTA CATGCGGAGT 
CCATCAGGGG ACATTTTCAA CCCAGAACAC TACCAAGTGA ATCAGGACAT GAGTTACCCT 
TTGAGTCACT ATTTCATTAC CTCTTCACAT AATACCTACC TCATGGGAGA TCAGCTGATG 
TCTCAGTCTA GGGTGGACAT GTATGCATGG GTGCTACAAT CCGGCTGTCG TTGTGTAGAA 
GTTGACTGCT GGGATGGGCC GGATGGTGAA CCAATTGTCC ACCATGGATA CACTTTGACT 
TCCAAAATTC TCTTCAAAGA TGTGATTGAA ACTATCAACA AATATGCCTT TATCAAGAAT 
GAGTACCCTG TTATCTTGTC AATTGAGAAC CACTGCAGTG TAGTTCAGCA GAAGAAGATG 
GCCCAATATC TTACAGAAAT TCTGGGTGAC AAATTGGATC TGTCTTCTGT GCACAATGAT 
GACTCTACCA GGCTCCCTTC ACCAGCAAGT CTTAAAGGGA AGATTCTGGT TAAGGGGAAG 
AAGCTTCCAG CCAACATTAG TGATGATGCA GAGGAAGGTG AAGTTTCTGA TGAGGACAGT 
GCTGATGAGA TTGATGATGA CTGTAAAATA ATGAATGGAG ATGCATCTAC TAATAGGAAA 
AGAGTGGAGA ATATAGCAAA GAAAAAACTT GACTCACTCA TAAAAGAATC CAAAATTCGG 
GACTGTGAAG ATCCAAACAA TTTTACAGTT ACCACTCTGC CATCATCTGG AAAGGCTGGT 
CAGAAATCAG ACAGTAAGAA GAATAAACTT GAGGATGATG CAGAATCTGG GGAAGACTTC 
AGTACCAATA AAAGGCATAG CCGAACATTA ATGGGCAGCT TTTCTAAACG CAAGAAAAAA 
GGAAGCAAAT TGAAAAAATC AACCAGTCTG GAAGAAGGTG AAGATGATGC TGATTCTCAA 
GGAAATGTAG CCAGAGGCTC TGTACATTAC ACCAGGATAA ACCGACAGAA GAAAACAATG 
AAGTTGTCCC GGGCACTCTC AGACCTGGTG AAATATACAA AGTCTGTCGG CATCCATGAT 
GTTGAAACTG AAATCTCTTC CAGCTGGCAG GTTTCATCTT TCAGTGAAAC AAAAGCCCAC 
CAGATACTGC AGCAAAAGCC AGCACAGTAT CTACGTTTCA ACCAACATCA GTTATCCCGC 
ATCTACCCAT CTTCTTACCG TGTAGACTCT AGCAACTACA ATCCTCAGCC ATTCTGGAAT 
GCTGGCTGCC AACTTGTGGC TTTAAACTAC CAGTCAGAGG GGAGAATGCT ACAACTGAAC 
CGGGCCAAAT TTAGTGCCAA TGGGAATTGT GGCTATGTCC TCAAACCAAA CTGCATGTGT 
CAAGGCATCT TTAATCCAAA TTCTGAAGAT CCACTGCCTG GTCAGTTGAA GAAACAGCTG 
GTTCTAAGAA TTATCAGTGG TCAACAGCTC CCAAAACCAC GGGATTCCAT GCTGGGAGAC 
AGAGGAGAGA TCATAGATCC ATTTGTTGAA GTAGAAGTTA TAGGCCTACC TGTCGATTGC 
TTCAAAGAAC AAACTAGAGT AGTTGATGAT AATGGATTTA ACCCAATGTG GGAAGAAACA 
TTGGTGTTCA CACTGCACAT GCCAGAGATT GCATTGATTC GGTTCCTTGT GTGGGATCAT 
GATCCAATTG GGCGGGATTT TATTGGACAA AGAACGATAG CTTTCAGCAG TATGATGCCA 
GGTTACCGAC ATGTATACCT AGAAGGTATA GAAGAGGCAT CCATCTTTGT TCATGTGGCT 
ATTAATGACA TTTGTGGTAA GGTCAAACAA GCGCTGGGCC TAAAAGGCCT ATTTCTCAGA 
AATCCAAAGC AGGCCTCCCT GGACAGTCAT GCTGCTGGTC AGCTCCATCG CAAGCATTCC 
TTTAGCAGCC ACATCTTGAG ACGGACAGCA AGTGCACCCA CCAAAAGCCA GAAGAAGAAG 
AAGGGCTTTC CTGAAATTGT TTTTGACACA AAAGACAACA GCTCTGAAGG GGCCAGTGAA 
GACCACGAAG CAGAAGCTGC CTCACAGCCT TGCTTTGAGC AAGAGCCAGA GTGTGCTTCT 
CTGTCACCAG CTCCCAGGGA TGGTGCCAGT GGGGAGGTAC ATGGCAAAGG GTTGAAAGGT 
AAGGCCCATG GTTCTCATAA AGCCAAAAGT CAAAGTTGTG CTCAAGGGGG TGCTGCTTTC 
AGTGAACCCA TACACAGGTC TAACAGCGTC CGACTACGGG AGCACCAGAG TGAGAAGCAA 
AGTGTCTTTG CACGCTGCGC CATCAACAGC TCTGGTAGGA TTGGAGTGGC CACCAACTGC 
ATGAAATGCA TGATTGGTTC TAAAGAAAGC CCAGATCTAG AAAGCAAGTG GAGTGACCAA 
CCTACCAGGC CTATTGCTAA AGATTTGCTT CACCATGATC AGTATAGCAG TATAACTGGA 
GAAGAGAGGT TAGAGCTTAA AAACTGGGGT ATGAAAGCTC AGTCCAGACC ACAGTCAGAT 
TTGCAGTCTT GCAGTAGCCC TGGAAATGCT GATGATCTAA CAAGGAGTAC CCAGTCTTTC 
GTGACCTCAG GGAAGAAAAA CGTTGAATCT AAATGTCATG GAATAAAAGC TCATTCCCGG 
CAGCGGTGGC AGTGCCAATC AGGTGGCAAG TATGGAAGCA CTATCAACTT GGTTGCAGCC 
AGCAATGGCT CCATATCCTC CAACTCCAGC AGTCTAGAAA GCCTTGGAAG CCCAGAATTT 
CCCAAGCGCT GTTCTGAAAC TTCTCGAAGG CAAGTGGGCA CCCTACAGAG GGAAATGAAT 
GCCTTATTCG TTCAAAAATT GGAGGAAATT CGAAGTAAAT CCCCTATATT CTTTACTGAT 
GTCGGCCGTT TCTTGATGCA GAGGTCAGTC ACATCTTTAT GCAGCCTAGA AACTATCACT 
GAAGAGCCTG TTATTGCAAA TGAAAGCCAT CATGCCAGCC TCTTCTTTCC TCTACCCGTT 
ACTCCTTACA GTGATTCTGA AGAAGGCTCT GTGTCTGATC ATTTCACTGA ATCTCAAACA 
AAGGGAAGCA ACCAATCGAA CCGGCTTCCA GAACCTCCGC TCACTAGAGA ACAAAGACAA 
ACTTTGGCTG CAGAAGACCA AGCGCAAATA GCCACAAAAG CAGCTGACAT TAATGTATTA 
GTCCAAGATG ATGAGAGGAC AAGCATGGCA GAACCTCTCA AAGAAGATGA TGGTTGGACG 
CAGGCATGCA GTGAAGCTGA AGATCAAGTT AATTTGGCAG CGCATCCTCA AAAAGCGTGG 
CTGGTGGTGG CAGAAGCTAA GAGTACTGGC CAGCCTGTTG AAAACTGCCA GAAGCAGAAT 
GACCAAACAG GGCAGATATT GAAAGACTTG AAAACCACTG CTGAATTGAA AAGCCAAAAA 
CCTGGTGCAG CAGCTGTCCT GCACCGAAAC AATGTGATTA ATGGCACACA TCCAACTTTG 
TCTCCAGATA TTTTCCAGAA AGCCCAGGCT GCACAGGCCC TACCTGTTTC TCAGACAAGC 
TCTTACATGT TAATAAGGAA GTCAAAGAGT GAAGGGCTGA AAAAGTCAGA CTCCTCAGCT 
TTAATAAAAA ATCCAAGTAG CCTATCTCCA GCAGCAGAAG TATACTTTGA TGCTACTATT 
AATGACCGGA TCTGGAGCAA GCTAGATTGC AGCAATCATC GTGACAGCAT GTCTTCCTCT 
TCCAGCATGT CCTCCAATGA CACTGTGATA GATCTGTCTT TACCTAATCT AGCTCGCAAA 
AGCCTTCCAG ATCTTGGCAT CCGTCATGAG AACTTTGAGT CCCTTGCCAT GAGAAAGAGC 
ATTCGCCCAC GTTCTGCTAC AGCGTCATGC AATGAAATGC CAATAGTGAG CAAGAGTAAA 
TCCAATCCTA ACCTGAGGTC TGACCAGCTG CCAGCGGATG AATTATGCCC CCGTCCTCTT 
GCAAGGAGTC CTCAGGATGC ATTCTCATCC ATTCCTAGGA GGCATACCTG GAGCAGGCTG 
TACATGGAAA GTCTCAGGCA ATCTTCTAAC AAATCCAAAG CACAGGATAA GGTGGGGAAT 
AACCAGGCAA AATCTAAGAG TCTTGGAGAC CTTACCTCTG ATGACATTGT GTGCACTTTT 
GAAAGCAAAT ATCGTAGCAT CAGCAGGAGT TTTGTCACTC GATCAATGCG TGAGCAGCGC 
CGCTCTGCAG GCCTGAGAAC CTCTGTCAAA CCCCAGGATG AATTGACTGA GCAGCTGAAG 
AAGCTAACGG CCTTTCAGCA GGAAAATGAT ATAACTTCCC CCATCAGTCT TGATCCAACT 
GAATCTGAAG AGGAAGCAGA GAGCTTAGGA CTCCTTCGCA GGTCATCATC TCGCAGTCAG 
AGCAGGGTGC GGTACATTGC CAACAGAGCC AAGCAAGCTC AGGAGAGACA GCGACTGCAA 
AGCCTGGGCC AGCTCAGTGG GAGTCCCATT GAAGAGAGAG GAAATCCAGA GGGAGCCTGC 
AGTGTTGCAA AAGCAGTTTG TATGGATATA GCATCTCCTG CTGTGTTTAC ATCCCAGCCT 
AAGAACCAAA CTGATTATAA TACTGACACA GAAGTCTTCT TTATGCTGAA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297081.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152567.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X7, mRNA.

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CloneID	OGa45174
Clone ID Related Accession (Same CDS sequence)	XM_015297081.1
Accession Version	XM_015297081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAAAC TGAATGTCAA CCTACCCCGA CAAAAAGTCA AGCAAATGTT TAAGGAGGCT 
GATACAGACG ATAACCAGGG CACGCTAGGC TTTGATGAGT TCTGCACCTT CTACAAGATG 
ATGTCAACAC GGCGTGATTT GTACTTGCTA ATGCTCACCT ACAGCAACCA CAAGGACTTC 
CTGGATACAG ATGACCTGAG ACGCTTTCTG GAGAATGAAC AAAAGATGAC AAATGTGACC 
AGAGAACACT GCCTGGAAAT CATCAACAAG TTTGAACCAT GCCTGGAAAA CAAGAAGGCG 
GGAGCTCTGG GGATTGATGG TTTCACCAAT TACATGCGGA GTCCATCAGG GGACATTTTC 
AACCCAGAAC ACTACCAAGT GAATCAGGAC ATGAGTTACC CTTTGAGTCA CTATTTCATT 
ACCTCTTCAC ATAATACCTA CCTCATGGGA GATCAGCTGA TGTCTCAGTC TAGGGTGGAC 
ATGTATGCAT GGGTGCTACA ATCCGGCTGT CGTTGTGTAG AAGTTGACTG CTGGGATGGG 
CCGGATGGTG AACCAATTGT CCACCATGGA TACACTTTGA CTTCCAAAAT TCTCTTCAAA 
GATGTGATTG AAACTATCAA CAAATATGCC TTTATCAAGA ATGAGTACCC TGTTATCTTG 
TCAATTGAGA ACCACTGCAG TGTAGTTCAG CAGAAGAAGA TGGCCCAATA TCTTACAGAA 
ATTCTGGGTG ACAAATTGGA TCTGTCTTCT GTGCACAATG ATGACTCTAC CAGGCTCCCT 
TCACCAGCAA GTCTTAAAGG GAAGATTCTG GTTAAGGGGA AGAAGCTTCC AGCCAACATT 
AGTGATGATG CAGAGGAAGG TGAAGTTTCT GATGAGGACA GTGCTGATGA GATTGATGAT 
GACTGTAAAA TAATGAATGG AGATGCATCT ACTAATAGGA AAAGAGTGGA GAATATAGCA 
AAGAAAAAAC TTGACTCACT CATAAAAGAA TCCAAAATTC GGGACTGTGA AGATCCAAAC 
AATTTTACAG TTACCACTCT GCCATCATCT GGAAAGGCTG GTCAGAAATC AGACAGTAAG 
AAGAATAAAC TTGAGGATGA TGCAGAATCT GGGGAAGACT TCAGTACCAA TAAAAGGCAT 
AGCCGAACAT TAATGGGCAG CTTTTCTAAA CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA 
TCAACCAGTC TGGAAGAAGG TGAAGATGAT GCTGATTCTC AAGGAAATGT AGCCAGAGGC 
TCTGTACATT ACACCAGGAT AAACCGACAG AAGAAAACAA TGAAGTTGTC CCGGGCACTC 
TCAGACCTGG TGAAATATAC AAAGTCTGTC GGCATCCATG ATGTTGAAAC TGAAATCTCT 
TCCAGCTGGC AGGTTTCATC TTTCAGTGAA ACAAAAGCCC ACCAGATACT GCAGCAAAAG 
CCAGCACAGT ATCTACGTTT CAACCAACAT CAGTTATCCC GCATCTACCC ATCTTCTTAC 
CGTGTAGACT CTAGCAACTA CAATCCTCAG CCATTCTGGA ATGCTGGCTG CCAACTTGTG 
GCTTTAAACT ACCAGTCAGA GGGGAGAATG CTACAACTGA ACCGGGCCAA ATTTAGTGCC 
AATGGGAATT GTGGCTATGT CCTCAAACCA AACTGCATGT GTCAAGGCAT CTTTAATCCA 
AATTCTGAAG ATCCACTGCC TGGTCAGTTG AAGAAACAGC TGGTTCTAAG AATTATCAGT 
GGTCAACAGC TCCCAAAACC ACGGGATTCC ATGCTGGGAG ACAGAGGAGA GATCATAGAT 
CCATTTGTTG AAGTAGAAGT TATAGGCCTA CCTGTCGATT GCTTCAAAGA ACAAACTAGA 
GTAGTTGATG ATAATGGATT TAACCCAATG TGGGAAGAAA CATTGGTGTT CACACTGCAC 
ATGCCAGAGA TTGCATTGAT TCGGTTCCTT GTGTGGGATC ATGATCCAAT TGGGCGGGAT 
TTTATTGGAC AAAGAACGAT AGCTTTCAGC AGTATGATGC CAGGTTACCG ACATGTATAC 
CTAGAAGGTA TAGAAGAGGC ATCCATCTTT GTTCATGTGG CTATTAATGA CATTTGTGGT 
AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC CTATTTCTCA GAAATCCAAA GCAGGCCTCC 
CTGGACAGTC ATGCTGCTGG TCAGCTCCAT CGCAAGCATT CCTTTAGCAG CCACATCTTG 
AGACGGACAG CAAGTGCACC CACCAAAAGC CAGAAGAAGA AGAAGGGCTT TCCTGAAATT 
GTTTTTGACA CAAAAGACAA CAGCTCTGAA GGGGCCAGTG AAGACCACGA AGCAGAAGCT 
GCCTCACAGC CTTGCTTTGA GCAAGAGCCA GAGTGTGCTT CTCTGTCACC AGCTCCCAGG 
GATGGTGCCA GTGGGGAGGT ACATGGCAAA GGGTTGAAAG GTAAGGCCCA TGGTTCTCAT 
AAAGCCAAAA GTCAAAGTTG TGCTCAAGGG GGTGCTGCTT TCAGTGAACC CATACACAGG 
TCTAACAGCG TCCGACTACG GGAGCACCAG AGTGAGAAGC AAAGTGTCTT TGCACGCTGC 
GCCATCAACA GCTCTGGTAG GATTGGAGTG GCCACCAACT GCATGAAATG CATGATTGGT 
TCTAAAGAAA GCCCAGATCT AGAAAGCAAG TGGAGTGACC AACCTACCAG GCCTATTGCT 
AAAGATTTGC TTCACCATGA TCAGTATAGC AGTATAACTG GAGAAGAGAG GTTAGAGCTT 
AAAAACTGGG GTATGAAAGC TCAGTCCAGA CCACAGTCAG ATTTGCAGTC TTGCAGTAGC 
CCTGGAAATG CTGATGATCT AACAAGGAGT ACCCAGTCTT TCGTGACCTC AGGGAAGAAA 
AACGTTGAAT CTAAATGTCA TGGAATAAAA GCTCATTCCC GGCAGCGGTG GCAGTGCCAA 
TCAGGTGGCA AGTATGGAAG CACTATCAAC TTGGTTGCAG CCAGCAATGG CTCCATATCC 
TCCAACTCCA GCAGTCTAGA AAGCCTTGGA AGCCCAGAAT TTCCCAAGCG CTGTTCTGAA 
ACTTCTCGAA GGCAAGTGGG CACCCTACAG AGGGAAATGA ATGCCTTATT CGTTCAAAAA 
TTGGAGGAAA TTCGAAGTAA ATCCCCTATA TTCTTTACTG ATGTCGGCCG TTTCTTGATG 
CAGAGGTCAG TCACATCTTT ATGCAGCCTA GAAACTATCA CTGAAGAGCC TGTTATTGCA 
AATGAAAGCC ATCATGCCAG CCTCTTCTTT CCTCTACCCG TTACTCCTTA CAGTGATTCT 
GAAGAAGGCT CTGTGTCTGA TCATTTCACT GAATCTCAAA CAAAGGGAAG CAACCAATCG 
AACCGGCTTC CAGAACCTCC GCTCACTAGA GAACAAAGAC AAACTTTGGC TGCAGAAGAC 
CAAGCGCAAA TAGCCACAAA AGCAGCTGAC ATTAATGTAT TAGTCCAAGA TGATGAGAGG 
ACAAGCATGG CAGAACCTCT CAAAGAAGAT GATGGTTGGA CGCAGGCATG CAGTGAAGCT 
GAAGATCAAG TTAATTTGGC AGCGCATCCT CAAAAAGCGT GGCTGGTGGT GGCAGAAGCT 
AAGAGTACTG GCCAGCCTGT TGAAAACTGC CAGAAGCAGA ATGACCAAAC AGGGCAGATA 
TTGAAAGACT TGAAAACCAC TGCTGAATTG AAAAGCCAAA AACCTGGTGC AGCAGCTGTC 
CTGCACCGAA ACAATGTGAT TAATGGCACA CATCCAACTT TGTCTCCAGA TATTTTCCAG 
AAAGCCCAGG CTGCACAGGC CCTACCTGTT TCTCAGACAA GCTCTTACAT GTTAATAAGG 
AAGTCAAAGA GTGAAGGGCT GAAAAAGTCA GACTCCTCAG CTTTAATAAA AAATCCAAGT 
AGCCTATCTC CAGCAGCAGA AGTATACTTT GATGCTACTA TTAATGACCG GATCTGGAGC 
AAGCTAGATT GCAGCAATCA TCGTGACAGC ATGTCTTCCT CTTCCAGCAT GTCCTCCAAT 
GACACTGTGA TAGATCTGTC TTTACCTAAT CTAGCTCGCA AAAGCCTTCC AGATCTTGGC 
ATCCGTCATG AGAACTTTGA GTCCCTTGCC ATGAGAAAGA GCATTCGCCC ACGTTCTGCT 
ACAGCGTCAT GCAATGAAAT GCCAATAGTG AGCAAGAGTA AATCCAATCC TAACCTGAGG 
TCTGACCAGC TGCCAGCGGA TGAATTATGC CCCCGTCCTC TTGCAAGGAG TCCTCAGGAT 
GCATTCTCAT CCATTCCTAG GAGGCATACC TGGAGCAGGC TGTACATGGA AAGTCTCAGG 
CAATCTTCTA ACAAATCCAA AGCACAGGAT AAGGTGGGGA ATAACCAGGC AAAATCTAAG 
AGTCTTGGAG ACCTTACCTC TGATGACATT GTGTGCACTT TTGAAAGCAA ATATCGTAGC 
ATCAGCAGGA GTTTTGTCAC TCGATCAATG CGTGAGCAGC GCCGCTCTGC AGGCCTGAGA 
ACCTCTGTCA AACCCCAGGA TGAATTGACT GAGCAGCTGA AGAAGCTAAC GGCCTTTCAG 
CAGGAAAATG ATATAACTTC CCCCATCAGT CTTGATCCAA CTGAATCTGA AGAGGAAGCA 
GAGAGCTTAG GACTCCTTCG CAGGTCATCA TCTCGCAGTC AGAGCAGGGT GCGGTACATT 
GCCAACAGAG CCAAGCAAGC TCAGGAGAGA CAGCGACTGC AAAGCCTGGG CCAGCTCAGT 
GGGAGTCCCA TTGAAGAGAG AGGAAATCCA GAGGGAGCCT GCAGTGTTGC AAAAGCAGTT 
TGTATGGATA TAGCATCTCC TGCTGTGTTT ACATCCCAGC CTAAGAACCA AACTGATTAT 
AATACTGACA CAGAAGTCTT CTTTATGCTG AAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152567.1
CDS	272..5170
Translation	MHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKGKAHGSHKAKSQSCAQGGAAFSEPIHRSNSVRLREHQSEKQSVFARCAINSSGRIGVATNCMKCMIGSKESPDLESKWSDQPTRPIAKDLLHHDQYSSITGEERLELKNWGMKAQSRPQSDLQSCSSPGNADDLTRSTQSFVTSGKKNVESKCHGIKAHSRQRWQCQSGGKYGSTINLVAASNGSISSNSSSLESLGSPEFPKRCSETSRRQVGTLQREMNALFVQKLEEIRSKSPIFFTDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297081.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297081.1

ORF Insert Sequence:

ATGCACAAAC TGAATGTCAA CCTACCCCGA CAAAAAGTCA AGCAAATGTT TAAGGAGGCT 
GATACAGACG ATAACCAGGG CACGCTAGGC TTTGATGAGT TCTGCACCTT CTACAAGATG 
ATGTCAACAC GGCGTGATTT GTACTTGCTA ATGCTCACCT ACAGCAACCA CAAGGACTTC 
CTGGATACAG ATGACCTGAG ACGCTTTCTG GAGAATGAAC AAAAGATGAC AAATGTGACC 
AGAGAACACT GCCTGGAAAT CATCAACAAG TTTGAACCAT GCCTGGAAAA CAAGAAGGCG 
GGAGCTCTGG GGATTGATGG TTTCACCAAT TACATGCGGA GTCCATCAGG GGACATTTTC 
AACCCAGAAC ACTACCAAGT GAATCAGGAC ATGAGTTACC CTTTGAGTCA CTATTTCATT 
ACCTCTTCAC ATAATACCTA CCTCATGGGA GATCAGCTGA TGTCTCAGTC TAGGGTGGAC 
ATGTATGCAT GGGTGCTACA ATCCGGCTGT CGTTGTGTAG AAGTTGACTG CTGGGATGGG 
CCGGATGGTG AACCAATTGT CCACCATGGA TACACTTTGA CTTCCAAAAT TCTCTTCAAA 
GATGTGATTG AAACTATCAA CAAATATGCC TTTATCAAGA ATGAGTACCC TGTTATCTTG 
TCAATTGAGA ACCACTGCAG TGTAGTTCAG CAGAAGAAGA TGGCCCAATA TCTTACAGAA 
ATTCTGGGTG ACAAATTGGA TCTGTCTTCT GTGCACAATG ATGACTCTAC CAGGCTCCCT 
TCACCAGCAA GTCTTAAAGG GAAGATTCTG GTTAAGGGGA AGAAGCTTCC AGCCAACATT 
AGTGATGATG CAGAGGAAGG TGAAGTTTCT GATGAGGACA GTGCTGATGA GATTGATGAT 
GACTGTAAAA TAATGAATGG AGATGCATCT ACTAATAGGA AAAGAGTGGA GAATATAGCA 
AAGAAAAAAC TTGACTCACT CATAAAAGAA TCCAAAATTC GGGACTGTGA AGATCCAAAC 
AATTTTACAG TTACCACTCT GCCATCATCT GGAAAGGCTG GTCAGAAATC AGACAGTAAG 
AAGAATAAAC TTGAGGATGA TGCAGAATCT GGGGAAGACT TCAGTACCAA TAAAAGGCAT 
AGCCGAACAT TAATGGGCAG CTTTTCTAAA CGCAAGAAAA AAGGAAGCAA ATTGAAAAAA 
TCAACCAGTC TGGAAGAAGG TGAAGATGAT GCTGATTCTC AAGGAAATGT AGCCAGAGGC 
TCTGTACATT ACACCAGGAT AAACCGACAG AAGAAAACAA TGAAGTTGTC CCGGGCACTC 
TCAGACCTGG TGAAATATAC AAAGTCTGTC GGCATCCATG ATGTTGAAAC TGAAATCTCT 
TCCAGCTGGC AGGTTTCATC TTTCAGTGAA ACAAAAGCCC ACCAGATACT GCAGCAAAAG 
CCAGCACAGT ATCTACGTTT CAACCAACAT CAGTTATCCC GCATCTACCC ATCTTCTTAC 
CGTGTAGACT CTAGCAACTA CAATCCTCAG CCATTCTGGA ATGCTGGCTG CCAACTTGTG 
GCTTTAAACT ACCAGTCAGA GGGGAGAATG CTACAACTGA ACCGGGCCAA ATTTAGTGCC 
AATGGGAATT GTGGCTATGT CCTCAAACCA AACTGCATGT GTCAAGGCAT CTTTAATCCA 
AATTCTGAAG ATCCACTGCC TGGTCAGTTG AAGAAACAGC TGGTTCTAAG AATTATCAGT 
GGTCAACAGC TCCCAAAACC ACGGGATTCC ATGCTGGGAG ACAGAGGAGA GATCATAGAT 
CCATTTGTTG AAGTAGAAGT TATAGGCCTA CCTGTCGATT GCTTCAAAGA ACAAACTAGA 
GTAGTTGATG ATAATGGATT TAACCCAATG TGGGAAGAAA CATTGGTGTT CACACTGCAC 
ATGCCAGAGA TTGCATTGAT TCGGTTCCTT GTGTGGGATC ATGATCCAAT TGGGCGGGAT 
TTTATTGGAC AAAGAACGAT AGCTTTCAGC AGTATGATGC CAGGTTACCG ACATGTATAC 
CTAGAAGGTA TAGAAGAGGC ATCCATCTTT GTTCATGTGG CTATTAATGA CATTTGTGGT 
AAGGTCAAAC AAGCGCTGGG CCTAAAAGGC CTATTTCTCA GAAATCCAAA GCAGGCCTCC 
CTGGACAGTC ATGCTGCTGG TCAGCTCCAT CGCAAGCATT CCTTTAGCAG CCACATCTTG 
AGACGGACAG CAAGTGCACC CACCAAAAGC CAGAAGAAGA AGAAGGGCTT TCCTGAAATT 
GTTTTTGACA CAAAAGACAA CAGCTCTGAA GGGGCCAGTG AAGACCACGA AGCAGAAGCT 
GCCTCACAGC CTTGCTTTGA GCAAGAGCCA GAGTGTGCTT CTCTGTCACC AGCTCCCAGG 
GATGGTGCCA GTGGGGAGGT ACATGGCAAA GGGTTGAAAG GTAAGGCCCA TGGTTCTCAT 
AAAGCCAAAA GTCAAAGTTG TGCTCAAGGG GGTGCTGCTT TCAGTGAACC CATACACAGG 
TCTAACAGCG TCCGACTACG GGAGCACCAG AGTGAGAAGC AAAGTGTCTT TGCACGCTGC 
GCCATCAACA GCTCTGGTAG GATTGGAGTG GCCACCAACT GCATGAAATG CATGATTGGT 
TCTAAAGAAA GCCCAGATCT AGAAAGCAAG TGGAGTGACC AACCTACCAG GCCTATTGCT 
AAAGATTTGC TTCACCATGA TCAGTATAGC AGTATAACTG GAGAAGAGAG GTTAGAGCTT 
AAAAACTGGG GTATGAAAGC TCAGTCCAGA CCACAGTCAG ATTTGCAGTC TTGCAGTAGC 
CCTGGAAATG CTGATGATCT AACAAGGAGT ACCCAGTCTT TCGTGACCTC AGGGAAGAAA 
AACGTTGAAT CTAAATGTCA TGGAATAAAA GCTCATTCCC GGCAGCGGTG GCAGTGCCAA 
TCAGGTGGCA AGTATGGAAG CACTATCAAC TTGGTTGCAG CCAGCAATGG CTCCATATCC 
TCCAACTCCA GCAGTCTAGA AAGCCTTGGA AGCCCAGAAT TTCCCAAGCG CTGTTCTGAA 
ACTTCTCGAA GGCAAGTGGG CACCCTACAG AGGGAAATGA ATGCCTTATT CGTTCAAAAA 
TTGGAGGAAA TTCGAAGTAA ATCCCCTATA TTCTTTACTG ATGTCGGCCG TTTCTTGATG 
CAGAGGTCAG TCACATCTTT ATGCAGCCTA GAAACTATCA CTGAAGAGCC TGTTATTGCA 
AATGAAAGCC ATCATGCCAG CCTCTTCTTT CCTCTACCCG TTACTCCTTA CAGTGATTCT 
GAAGAAGGCT CTGTGTCTGA TCATTTCACT GAATCTCAAA CAAAGGGAAG CAACCAATCG 
AACCGGCTTC CAGAACCTCC GCTCACTAGA GAACAAAGAC AAACTTTGGC TGCAGAAGAC 
CAAGCGCAAA TAGCCACAAA AGCAGCTGAC ATTAATGTAT TAGTCCAAGA TGATGAGAGG 
ACAAGCATGG CAGAACCTCT CAAAGAAGAT GATGGTTGGA CGCAGGCATG CAGTGAAGCT 
GAAGATCAAG TTAATTTGGC AGCGCATCCT CAAAAAGCGT GGCTGGTGGT GGCAGAAGCT 
AAGAGTACTG GCCAGCCTGT TGAAAACTGC CAGAAGCAGA ATGACCAAAC AGGGCAGATA 
TTGAAAGACT TGAAAACCAC TGCTGAATTG AAAAGCCAAA AACCTGGTGC AGCAGCTGTC 
CTGCACCGAA ACAATGTGAT TAATGGCACA CATCCAACTT TGTCTCCAGA TATTTTCCAG 
AAAGCCCAGG CTGCACAGGC CCTACCTGTT TCTCAGACAA GCTCTTACAT GTTAATAAGG 
AAGTCAAAGA GTGAAGGGCT GAAAAAGTCA GACTCCTCAG CTTTAATAAA AAATCCAAGT 
AGCCTATCTC CAGCAGCAGA AGTATACTTT GATGCTACTA TTAATGACCG GATCTGGAGC 
AAGCTAGATT GCAGCAATCA TCGTGACAGC ATGTCTTCCT CTTCCAGCAT GTCCTCCAAT 
GACACTGTGA TAGATCTGTC TTTACCTAAT CTAGCTCGCA AAAGCCTTCC AGATCTTGGC 
ATCCGTCATG AGAACTTTGA GTCCCTTGCC ATGAGAAAGA GCATTCGCCC ACGTTCTGCT 
ACAGCGTCAT GCAATGAAAT GCCAATAGTG AGCAAGAGTA AATCCAATCC TAACCTGAGG 
TCTGACCAGC TGCCAGCGGA TGAATTATGC CCCCGTCCTC TTGCAAGGAG TCCTCAGGAT 
GCATTCTCAT CCATTCCTAG GAGGCATACC TGGAGCAGGC TGTACATGGA AAGTCTCAGG 
CAATCTTCTA ACAAATCCAA AGCACAGGAT AAGGTGGGGA ATAACCAGGC AAAATCTAAG 
AGTCTTGGAG ACCTTACCTC TGATGACATT GTGTGCACTT TTGAAAGCAA ATATCGTAGC 
ATCAGCAGGA GTTTTGTCAC TCGATCAATG CGTGAGCAGC GCCGCTCTGC AGGCCTGAGA 
ACCTCTGTCA AACCCCAGGA TGAATTGACT GAGCAGCTGA AGAAGCTAAC GGCCTTTCAG 
CAGGAAAATG ATATAACTTC CCCCATCAGT CTTGATCCAA CTGAATCTGA AGAGGAAGCA 
GAGAGCTTAG GACTCCTTCG CAGGTCATCA TCTCGCAGTC AGAGCAGGGT GCGGTACATT 
GCCAACAGAG CCAAGCAAGC TCAGGAGAGA CAGCGACTGC AAAGCCTGGG CCAGCTCAGT 
GGGAGTCCCA TTGAAGAGAG AGGAAATCCA GAGGGAGCCT GCAGTGTTGC AAAAGCAGTT 
TGTATGGATA TAGCATCTCC TGCTGTGTTT ACATCCCAGC CTAAGAACCA AACTGATTAT 
AATACTGACA CAGAAGTCTT CTTTATGCTG AAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297082.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152568.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X8, mRNA.

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CloneID	OGa45175
Clone ID Related Accession (Same CDS sequence)	XM_015297082.1 , XM_025142607.1 , XM_015297082.2 , XM_025142606.1 , XM_025142605.1 , XM_025142608.1
Accession Version	XM_015297082.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152568.1
CDS	528..5288
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297082.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297082.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142607.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998375.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

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CloneID	OGa45175
Clone ID Related Accession (Same CDS sequence)	XM_015297082.1 , XM_025142607.1 , XM_015297082.2 , XM_025142606.1 , XM_025142605.1 , XM_025142608.1
Accession Version	XM_025142607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998375.1
CDS	754..5514
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142607.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142607.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_015297082.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015152568.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

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CloneID	OGa45175
Clone ID Related Accession (Same CDS sequence)	XM_015297082.1 , XM_025142607.1 , XM_015297082.2 , XM_025142606.1 , XM_025142605.1 , XM_025142608.1
Accession Version	XM_015297082.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015297082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015152568.1
CDS	647..5407
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_015297082.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015297082.2

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142606.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998374.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa45175
Clone ID Related Accession (Same CDS sequence)	XM_015297082.1 , XM_025142607.1 , XM_015297082.2 , XM_025142606.1 , XM_025142605.1 , XM_025142608.1
Accession Version	XM_025142606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998374.1
CDS	921..5681
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142606.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142606.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142605.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998373.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa45175
Clone ID Related Accession (Same CDS sequence)	XM_015297082.1 , XM_025142607.1 , XM_015297082.2 , XM_025142606.1 , XM_025142605.1 , XM_025142608.1
Accession Version	XM_025142605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998373.1
CDS	949..5709
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142605.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142605.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_025142608.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998376.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

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CloneID	OGa45175
Clone ID Related Accession (Same CDS sequence)	XM_015297082.1 , XM_025142607.1 , XM_015297082.2 , XM_025142606.1 , XM_025142605.1 , XM_025142608.1
Accession Version	XM_025142608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998376.1
CDS	645..5405
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKVDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_025142608.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142608.1

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGT AGATGTCGGC CGTTTCTTGA TGCAGAGGTC AGTCACATCT 
TTATGCAGCC TAGAAACTAT CACTGAAGAG CCTGTTATTG CAAATGAAAG CCATCATGCC 
AGCCTCTTCT TTCCTCTACC CGTTACTCCT TACAGTGATT CTGAAGAAGG CTCTGTGTCT 
GATCATTTCA CTGAATCTCA AACAAAGGGA AGCAACCAAT CGAACCGGCT TCCAGAACCT 
CCGCTCACTA GAGAACAAAG ACAAACTTTG GCTGCAGAAG ACCAAGCGCA AATAGCCACA 
AAAGCAGCTG ACATTAATGT ATTAGTCCAA GATGATGAGA GGACAAGCAT GGCAGAACCT 
CTCAAAGAAG ATGATGGTTG GACGCAGGCA TGCAGTGAAG CTGAAGATCA AGTTAATTTG 
GCAGCGCATC CTCAAAAAGC GTGGCTGGTG GTGGCAGAAG CTAAGAGTAC TGGCCAGCCT 
GTTGAAAACT GCCAGAAGCA GAATGACCAA ACAGGGCAGA TATTGAAAGA CTTGAAAACC 
ACTGCTGAAT TGAAAAGCCA AAAACCTGGT GCAGCAGCTG TCCTGCACCG AAACAATGTG 
ATTAATGGCA CACATCCAAC TTTGTCTCCA GATATTTTCC AGAAAGCCCA GGCTGCACAG 
GCCCTACCTG TTTCTCAGAC AAGCTCTTAC ATGTTAATAA GGAAGTCAAA GAGTGAAGGG 
CTGAAAAAGT CAGACTCCTC AGCTTTAATA AAAAATCCAA GTAGCCTATC TCCAGCAGCA 
GAAGTATACT TTGATGCTAC TATTAATGAC CGGATCTGGA GCAAGCTAGA TTGCAGCAAT 
CATCGTGACA GCATGTCTTC CTCTTCCAGC ATGTCCTCCA ATGACACTGT GATAGATCTG 
TCTTTACCTA ATCTAGCTCG CAAAAGCCTT CCAGATCTTG GCATCCGTCA TGAGAACTTT 
GAGTCCCTTG CCATGAGAAA GAGCATTCGC CCACGTTCTG CTACAGCGTC ATGCAATGAA 
ATGCCAATAG TGAGCAAGAG TAAATCCAAT CCTAACCTGA GGTCTGACCA GCTGCCAGCG 
GATGAATTAT GCCCCCGTCC TCTTGCAAGG AGTCCTCAGG ATGCATTCTC ATCCATTCCT 
AGGAGGCATA CCTGGAGCAG GCTGTACATG GAAAGTCTCA GGCAATCTTC TAACAAATCC 
AAAGCACAGG ATAAGGTGGG GAATAACCAG GCAAAATCTA AGAGTCTTGG AGACCTTACC 
TCTGATGACA TTGTGTGCAC TTTTGAAAGC AAATATCGTA GCATCAGCAG GAGTTTTGTC 
ACTCGATCAA TGCGTGAGCA GCGCCGCTCT GCAGGCCTGA GAACCTCTGT CAAACCCCAG 
GATGAATTGA CTGAGCAGCT GAAGAAGCTA ACGGCCTTTC AGCAGGAAAA TGATATAACT 
TCCCCCATCA GTCTTGATCC AACTGAATCT GAAGAGGAAG CAGAGAGCTT AGGACTCCTT 
CGCAGGTCAT CATCTCGCAG TCAGAGCAGG GTGCGGTACA TTGCCAACAG AGCCAAGCAA 
GCTCAGGAGA GACAGCGACT GCAAAGCCTG GGCCAGCTCA GTGGGAGTCC CATTGAAGAG 
AGAGGAAATC CAGAGGGAGC CTGCAGTGTT GCAAAAGCAG TTTGTATGGA TATAGCATCT 
CCTGCTGTGT TTACATCCCA GCCTAAGAAC CAAACTGATT ATAATACTGA CACAGAAGTC 
TTCTTTATGC TGAAACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_004947382.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947439.1 Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X9, mRNA.

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CloneID	OGa18165
Clone ID Related Accession (Same CDS sequence)	XM_004947382.2 , XM_004947382.3
Accession Version	XM_004947382.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455994.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004947382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947439.1
CDS	527..5284
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_004947382.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C, eta 2 (PLCH2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004947382.2

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_004947382.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947439.1 Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.

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CloneID	OGa18165
Clone ID Related Accession (Same CDS sequence)	XM_004947382.2 , XM_004947382.3
Accession Version	XM_004947382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006108.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004947382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947439.1
CDS	643..5400
Translation	MALPSPKSIAGDLTQREELMLHNESCSHFLSTNVITVVERCMSSMQAGTQMIKLRGGSKGLVRFYYLDEHKSCIRWRPSRKNERAKISIDSIQEVCEGKQSEIFQRYADGSFDPNCCFSIYYGDHMESLDLVSPSGDEARTWITGLKYLMAGISDEDSLSKRQRTRDQWLKQTFDEADKNGDGCLSITEVLQLMHKLNVNLPRQKVKQMFKEADTDDNQGTLGFDEFCTFYKMMSTRRDLYLLMLTYSNHKDFLDTDDLRRFLENEQKMTNVTREHCLEIINKFEPCLENKKAGALGIDGFTNYMRSPSGDIFNPEHYQVNQDMSYPLSHYFITSSHNTYLMGDQLMSQSRVDMYAWVLQSGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTEILGDKLDLSSVHNDDSTRLPSPASLKGKILVKGKKLPANISDDAEEGEVSDEDSADEIDDDCKIMNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNNFTVTTLPSSGKAGQKSDSKKNKLEDDAESGEDFSTNKRHSRTLMGSFSKRKKKGSKLKKSTSLEEGEDDADSQGNVARGSVHYTRINRQKKTMKLSRALSDLVKYTKSVGIHDVETEISSSWQVSSFSETKAHQILQQKPAQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQLVALNYQSEGRMLQLNRAKFSANGNCGYVLKPNCMCQGIFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEVIGLPVDCFKEQTRVVDDNGFNPMWEETLVFTLHMPEIALIRFLVWDHDPIGRDFIGQRTIAFSSMMPGYRHVYLEGIEEASIFVHVAINDICGKVKQALGLKGLFLRNPKQASLDSHAAGQLHRKHSFSSHILRRTASAPTKSQKKKKGFPEIVFDTKDNSSEGASEDHEAEAASQPCFEQEPECASLSPAPRDGASGEVHGKGLKDVGRFLMQRSVTSLCSLETITEEPVIANESHHASLFFPLPVTPYSDSEEGSVSDHFTESQTKGSNQSNRLPEPPLTREQRQTLAAEDQAQIATKAADINVLVQDDERTSMAEPLKEDDGWTQACSEAEDQVNLAAHPQKAWLVVAEAKSTGQPVENCQKQNDQTGQILKDLKTTAELKSQKPGAAAVLHRNNVINGTHPTLSPDIFQKAQAAQALPVSQTSSYMLIRKSKSEGLKKSDSSALIKNPSSLSPAAEVYFDATINDRIWSKLDCSNHRDSMSSSSSMSSNDTVIDLSLPNLARKSLPDLGIRHENFESLAMRKSIRPRSATASCNEMPIVSKSKSNPNLRSDQLPADELCPRPLARSPQDAFSSIPRRHTWSRLYMESLRQSSNKSKAQDKVGNNQAKSKSLGDLTSDDIVCTFESKYRSISRSFVTRSMREQRRSAGLRTSVKPQDELTEQLKKLTAFQQENDITSPISLDPTESEEEAESLGLLRRSSSRSQSRVRYIANRAKQAQERQRLQSLGQLSGSPIEERGNPEGACSVAKAVCMDIASPAVFTSQPKNQTDYNTDTEVFFMLKL

Target ORF information:

RefSeq Version	XM_004947382.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004947382.3

ORF Insert Sequence:

ATGGCTCTGC CTTCTCCAAA ATCAATAGCA GGAGATCTGA CACAAAGAGA AGAACTCATG 
CTCCATAATG AAAGCTGTTC TCATTTCCTC TCCACAAACG TCATCACAGT AGTGGAAAGA 
TGTATGAGCT CCATGCAAGC TGGTACCCAA ATGATCAAAC TCCGTGGTGG TTCCAAAGGG 
TTGGTCCGCT TCTATTACCT GGATGAGCAC AAGTCATGCA TTCGCTGGAG ACCCTCCCGA 
AAGAATGAGA GAGCCAAAAT TTCCATCGAC TCTATTCAAG AGGTTTGTGA GGGGAAGCAA 
TCGGAGATCT TTCAGCGCTA TGCTGATGGA AGTTTTGATC CTAACTGCTG CTTCAGCATC 
TACTATGGAG ATCATATGGA ATCCCTTGAC CTGGTGTCTC CCAGTGGAGA TGAAGCACGT 
ACTTGGATCA CAGGGCTAAA ATATCTGATG GCAGGGATCA GTGATGAAGA CAGCCTCTCA 
AAACGCCAAA GAACCAGAGA CCAGTGGTTG AAGCAGACAT TTGATGAAGC AGATAAAAAT 
GGTGATGGCT GTCTGAGTAT TACTGAAGTG CTCCAACTAA TGCACAAACT GAATGTCAAC 
CTACCCCGAC AAAAAGTCAA GCAAATGTTT AAGGAGGCTG ATACAGACGA TAACCAGGGC 
ACGCTAGGCT TTGATGAGTT CTGCACCTTC TACAAGATGA TGTCAACACG GCGTGATTTG 
TACTTGCTAA TGCTCACCTA CAGCAACCAC AAGGACTTCC TGGATACAGA TGACCTGAGA 
CGCTTTCTGG AGAATGAACA AAAGATGACA AATGTGACCA GAGAACACTG CCTGGAAATC 
ATCAACAAGT TTGAACCATG CCTGGAAAAC AAGAAGGCGG GAGCTCTGGG GATTGATGGT 
TTCACCAATT ACATGCGGAG TCCATCAGGG GACATTTTCA ACCCAGAACA CTACCAAGTG 
AATCAGGACA TGAGTTACCC TTTGAGTCAC TATTTCATTA CCTCTTCACA TAATACCTAC 
CTCATGGGAG ATCAGCTGAT GTCTCAGTCT AGGGTGGACA TGTATGCATG GGTGCTACAA 
TCCGGCTGTC GTTGTGTAGA AGTTGACTGC TGGGATGGGC CGGATGGTGA ACCAATTGTC 
CACCATGGAT ACACTTTGAC TTCCAAAATT CTCTTCAAAG ATGTGATTGA AACTATCAAC 
AAATATGCCT TTATCAAGAA TGAGTACCCT GTTATCTTGT CAATTGAGAA CCACTGCAGT 
GTAGTTCAGC AGAAGAAGAT GGCCCAATAT CTTACAGAAA TTCTGGGTGA CAAATTGGAT 
CTGTCTTCTG TGCACAATGA TGACTCTACC AGGCTCCCTT CACCAGCAAG TCTTAAAGGG 
AAGATTCTGG TTAAGGGGAA GAAGCTTCCA GCCAACATTA GTGATGATGC AGAGGAAGGT 
GAAGTTTCTG ATGAGGACAG TGCTGATGAG ATTGATGATG ACTGTAAAAT AATGAATGGA 
GATGCATCTA CTAATAGGAA AAGAGTGGAG AATATAGCAA AGAAAAAACT TGACTCACTC 
ATAAAAGAAT CCAAAATTCG GGACTGTGAA GATCCAAACA ATTTTACAGT TACCACTCTG 
CCATCATCTG GAAAGGCTGG TCAGAAATCA GACAGTAAGA AGAATAAACT TGAGGATGAT 
GCAGAATCTG GGGAAGACTT CAGTACCAAT AAAAGGCATA GCCGAACATT AATGGGCAGC 
TTTTCTAAAC GCAAGAAAAA AGGAAGCAAA TTGAAAAAAT CAACCAGTCT GGAAGAAGGT 
GAAGATGATG CTGATTCTCA AGGAAATGTA GCCAGAGGCT CTGTACATTA CACCAGGATA 
AACCGACAGA AGAAAACAAT GAAGTTGTCC CGGGCACTCT CAGACCTGGT GAAATATACA 
AAGTCTGTCG GCATCCATGA TGTTGAAACT GAAATCTCTT CCAGCTGGCA GGTTTCATCT 
TTCAGTGAAA CAAAAGCCCA CCAGATACTG CAGCAAAAGC CAGCACAGTA TCTACGTTTC 
AACCAACATC AGTTATCCCG CATCTACCCA TCTTCTTACC GTGTAGACTC TAGCAACTAC 
AATCCTCAGC CATTCTGGAA TGCTGGCTGC CAACTTGTGG CTTTAAACTA CCAGTCAGAG 
GGGAGAATGC TACAACTGAA CCGGGCCAAA TTTAGTGCCA ATGGGAATTG TGGCTATGTC 
CTCAAACCAA ACTGCATGTG TCAAGGCATC TTTAATCCAA ATTCTGAAGA TCCACTGCCT 
GGTCAGTTGA AGAAACAGCT GGTTCTAAGA ATTATCAGTG GTCAACAGCT CCCAAAACCA 
CGGGATTCCA TGCTGGGAGA CAGAGGAGAG ATCATAGATC CATTTGTTGA AGTAGAAGTT 
ATAGGCCTAC CTGTCGATTG CTTCAAAGAA CAAACTAGAG TAGTTGATGA TAATGGATTT 
AACCCAATGT GGGAAGAAAC ATTGGTGTTC ACACTGCACA TGCCAGAGAT TGCATTGATT 
CGGTTCCTTG TGTGGGATCA TGATCCAATT GGGCGGGATT TTATTGGACA AAGAACGATA 
GCTTTCAGCA GTATGATGCC AGGTTACCGA CATGTATACC TAGAAGGTAT AGAAGAGGCA 
TCCATCTTTG TTCATGTGGC TATTAATGAC ATTTGTGGTA AGGTCAAACA AGCGCTGGGC 
CTAAAAGGCC TATTTCTCAG AAATCCAAAG CAGGCCTCCC TGGACAGTCA TGCTGCTGGT 
CAGCTCCATC GCAAGCATTC CTTTAGCAGC CACATCTTGA GACGGACAGC AAGTGCACCC 
ACCAAAAGCC AGAAGAAGAA GAAGGGCTTT CCTGAAATTG TTTTTGACAC AAAAGACAAC 
AGCTCTGAAG GGGCCAGTGA AGACCACGAA GCAGAAGCTG CCTCACAGCC TTGCTTTGAG 
CAAGAGCCAG AGTGTGCTTC TCTGTCACCA GCTCCCAGGG ATGGTGCCAG TGGGGAGGTA 
CATGGCAAAG GGTTGAAAGA TGTCGGCCGT TTCTTGATGC AGAGGTCAGT CACATCTTTA 
TGCAGCCTAG AAACTATCAC TGAAGAGCCT GTTATTGCAA ATGAAAGCCA TCATGCCAGC 
CTCTTCTTTC CTCTACCCGT TACTCCTTAC AGTGATTCTG AAGAAGGCTC TGTGTCTGAT 
CATTTCACTG AATCTCAAAC AAAGGGAAGC AACCAATCGA ACCGGCTTCC AGAACCTCCG 
CTCACTAGAG AACAAAGACA AACTTTGGCT GCAGAAGACC AAGCGCAAAT AGCCACAAAA 
GCAGCTGACA TTAATGTATT AGTCCAAGAT GATGAGAGGA CAAGCATGGC AGAACCTCTC 
AAAGAAGATG ATGGTTGGAC GCAGGCATGC AGTGAAGCTG AAGATCAAGT TAATTTGGCA 
GCGCATCCTC AAAAAGCGTG GCTGGTGGTG GCAGAAGCTA AGAGTACTGG CCAGCCTGTT 
GAAAACTGCC AGAAGCAGAA TGACCAAACA GGGCAGATAT TGAAAGACTT GAAAACCACT 
GCTGAATTGA AAAGCCAAAA ACCTGGTGCA GCAGCTGTCC TGCACCGAAA CAATGTGATT 
AATGGCACAC ATCCAACTTT GTCTCCAGAT ATTTTCCAGA AAGCCCAGGC TGCACAGGCC 
CTACCTGTTT CTCAGACAAG CTCTTACATG TTAATAAGGA AGTCAAAGAG TGAAGGGCTG 
AAAAAGTCAG ACTCCTCAGC TTTAATAAAA AATCCAAGTA GCCTATCTCC AGCAGCAGAA 
GTATACTTTG ATGCTACTAT TAATGACCGG ATCTGGAGCA AGCTAGATTG CAGCAATCAT 
CGTGACAGCA TGTCTTCCTC TTCCAGCATG TCCTCCAATG ACACTGTGAT AGATCTGTCT 
TTACCTAATC TAGCTCGCAA AAGCCTTCCA GATCTTGGCA TCCGTCATGA GAACTTTGAG 
TCCCTTGCCA TGAGAAAGAG CATTCGCCCA CGTTCTGCTA CAGCGTCATG CAATGAAATG 
CCAATAGTGA GCAAGAGTAA ATCCAATCCT AACCTGAGGT CTGACCAGCT GCCAGCGGAT 
GAATTATGCC CCCGTCCTCT TGCAAGGAGT CCTCAGGATG CATTCTCATC CATTCCTAGG 
AGGCATACCT GGAGCAGGCT GTACATGGAA AGTCTCAGGC AATCTTCTAA CAAATCCAAA 
GCACAGGATA AGGTGGGGAA TAACCAGGCA AAATCTAAGA GTCTTGGAGA CCTTACCTCT 
GATGACATTG TGTGCACTTT TGAAAGCAAA TATCGTAGCA TCAGCAGGAG TTTTGTCACT 
CGATCAATGC GTGAGCAGCG CCGCTCTGCA GGCCTGAGAA CCTCTGTCAA ACCCCAGGAT 
GAATTGACTG AGCAGCTGAA GAAGCTAACG GCCTTTCAGC AGGAAAATGA TATAACTTCC 
CCCATCAGTC TTGATCCAAC TGAATCTGAA GAGGAAGCAG AGAGCTTAGG ACTCCTTCGC 
AGGTCATCAT CTCGCAGTCA GAGCAGGGTG CGGTACATTG CCAACAGAGC CAAGCAAGCT 
CAGGAGAGAC AGCGACTGCA AAGCCTGGGC CAGCTCAGTG GGAGTCCCAT TGAAGAGAGA 
GGAAATCCAG AGGGAGCCTG CAGTGTTGCA AAAGCAGTTT GTATGGATAT AGCATCTCCT 
GCTGTGTTTA CATCCCAGCC TAAGAACCAA ACTGATTATA ATACTGACAC AGAAGTCTTC 
TTTATGCTGA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCH2
Entrez Gene ID	428180
Full Name	phospholipase C eta 2
General protein information	Preferred Names phospholipase C eta 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	PLCH2	NP_055453.2
Pan troglodytes (chimpanzee)	PLCH2	XP_003307816.2
Mus musculus (house mouse)	Plch2	NP_001106831.1
Oryza sativa (rice)	Os03g0289300	NP_001049792.2
	PLCH2	XP_001085424.2
Arabidopsis thaliana (thale cress)	AT2G40116	NP_850327.2
Xenopus tropicalis (tropical clawed frog)	LOC100487353	XP_002936604.2
Canis lupus familiaris (dog)	PLCH2	XP_005620480.1
Bos taurus (cattle)	PLCH2	XP_005217252.1
Rattus norvegicus (Norway rat)	Plch2	XP_006239682.1
Danio rerio (zebrafish)	plch2	XP_689411.6
Gallus gallus (chicken)	PLCH2	XP_425738.3
Oryza sativa (rice)	Os07g0694000	NP_001060735.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PLCH2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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