PLCH2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCH2
Entrez Gene ID	9651
Full Name	phospholipase C eta 2
Synonyms	PLC-L4,PLC-eta2,PLCL4,PLCeta2
General protein information	Preferred Names phospholipase C eta 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 phosphoinositide phospholipase C-eta-2 phosphoinositide phospholipase C-like 4 phospholipase C-eta2 phospholipase C-like 4 phospholipase C-like protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.32
Summary	PLCH2 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave PtdIns(4,5) P2 to generate second messengers inositol 1,4,5-trisphosphate and diacylglycerol (Zhou et al., 2005 [PubMed 16107206]).[supplied by OMIM, Jun 2009].
Disorder MIM:	612836

mRNA and Protein(s)

mRNA	Protein	Name
XM_017002871.1	XP_016858360.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_024451058.1	XP_024306826.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
XM_024451059.1	XP_024306827.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_017002872.1	XP_016858361.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
XM_024451062.1	XP_024306830.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
XM_024451063.1	XP_024306831.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X8
NM_001303012.1	NP_001289941.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 2
NM_014638.4	NP_055453.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 1
XM_017002870.1	XP_016858359.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_024451060.1	XP_024306828.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_017002873.1	XP_016858362.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
XM_024451061.1	XP_024306829.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
NM_014638.3	NP_055453.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 1
NM_001303012.2	NP_001289941.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 2
XM_024451064.1	XP_024306832.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X10
XM_011542455.2	XP_011540757.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_011542456.2	XP_011540758.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
NM_001303013.1	NP_001289942.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 3
XM_017002874.1	XP_016858363.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X9

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLCH2	NP_055453.2
Pan troglodytes (chimpanzee)	PLCH2	XP_003307816.2
Mus musculus (house mouse)	Plch2	NP_001106831.1
Oryza sativa (rice)	Os03g0289300	NP_001049792.2
	PLCH2	XP_001085424.2
Arabidopsis thaliana (thale cress)	AT2G40116	NP_850327.2
Xenopus tropicalis (tropical clawed frog)	LOC100487353	XP_002936604.2
Canis lupus familiaris (dog)	PLCH2	XP_005620480.1
Bos taurus (cattle)	PLCH2	XP_005217252.1
Rattus norvegicus (Norway rat)	Plch2	XP_006239682.1
Danio rerio (zebrafish)	plch2	XP_689411.6
Gallus gallus (chicken)	PLCH2	XP_425738.3
Oryza sativa (rice)	Os07g0694000	NP_001060735.1

Gene Ontology
Bibliography

Related articles in PubMed

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Jostins L, Ripke S, Weersma RK, Duerr RH, McGovern DP, Hui KY, Lee JC, Schumm LP, Sharma Y, Anderson CA, Essers J, Mitrovic M, Ning K, Cleynen I, Theatre E, Spain SL, Raychaudhuri S, Goyette P, Wei Z, Abraham C, Achkar JP, Ahmad T, Amininejad L, Ananthakrishnan AN, Andersen V, Andrews JM, Baidoo L, Balschun T, Bampton PA, Bitton A, Boucher G, Brand S, B?ning C, Cohain A, Cichon S, D'Amato M, De Jong D, Devaney KL, Dubinsky M, Edwards C, Ellinghaus D, Ferguson LR, Franchimont D, Fransen K, Gearry R, Georges M, Gieger C, Glas J, Haritunians T, Hart A, Hawkey C, Hedl M, Hu X, Karlsen TH, Kupcinskas L, Kugathasan S, Latiano A, Laukens D, Lawrance IC, Lees CW, Louis E, Mahy G, Mansfield J, Morgan AR, Mowat C, Newman W, Palmieri O, Ponsioen CY, Potocnik U, Prescott NJ, Regueiro M, Rotter JI, Russell RK, Sanderson JD, Sans M, Satsangi J, Schreiber S, Simms LA, Sventoraityte J, Targan SR, Taylor KD, Tremelling M, Verspaget HW, De Vos M, Wijmenga C, Wilson DC, Winkelmann J, Xavier RJ, Zeissig S, Zhang B, Zhang CK, Zhao H, , Silverberg MS, Annese V, Hakonarson H, Brant SR, Radford-Smith G, Mathew CG, Rioux JD, Schadt EE, Daly MJ, Franke A, Parkes M, Vermeire S, Barrett JC, Cho JH
Nature491(7422)119-24(2012 Nov)

A genome-wide association study in Chinese men identifies three risk loci for non-obstructive azoospermia.
Hu Z, Xia Y, Guo X, Dai J, Li H, Hu H, Jiang Y, Lu F, Wu Y, Yang X, Li H, Yao B, Lu C, Xiong C, Li Z, Gui Y, Liu J, Zhou Z, Shen H, Wang X, Sha J
Nature genetics44(2)183-6(2011 Dec)

Meta-analysis identifies 29 additional ulcerative colitis risk loci, increasing the number of confirmed associations to 47.
Anderson CA, Boucher G, Lees CW, Franke A, D'Amato M, Taylor KD, Lee JC, Goyette P, Imielinski M, Latiano A, Lagac? C, Scott R, Amininejad L, Bumpstead S, Baidoo L, Baldassano RN, Barclay M, Bayless TM, Brand S, B?ning C, Colombel JF, Denson LA, De Vos M, Dubinsky M, Edwards C, Ellinghaus D, Fehrmann RS, Floyd JA, Florin T, Franchimont D, Franke L, Georges M, Glas J, Glazer NL, Guthery SL, Haritunians T, Hayward NK, Hugot JP, Jobin G, Laukens D, Lawrance I, L?mann M, Levine A, Libioulle C, Louis E, McGovern DP, Milla M, Montgomery GW, Morley KI, Mowat C, Ng A, Newman W, Ophoff RA, Papi L, Palmieri O, Peyrin-Biroulet L, Pan?s J, Phillips A, Prescott NJ, Proctor DD, Roberts R, Russell R, Rutgeerts P, Sanderson J, Sans M, Schumm P, Seibold F, Sharma Y, Simms LA, Seielstad M, Steinhart AH, Targan SR, van den Berg LH, Vatn M, Verspaget H, Walters T, Wijmenga C, Wilson DC, Westra HJ, Xavier RJ, Zhao ZZ, Ponsioen CY, Andersen V, Torkvist L, Gazouli M, Anagnou NP, Karlsen TH, Kupcinskas L, Sventoraityte J, Mansfield JC, Kugathasan S, Silverberg MS, Halfvarson J, Rotter JI, Mathew CG, Griffiths AM, Gearry R, Ahmad T, Brant SR, Chamaillard M, Satsangi J, Cho JH, Schreiber S, Daly MJ, Barrett JC, Parkes M, Annese V, Hakonarson H, Radford-Smith G, Duerr RH, Vermeire S, Weersma RK, Rioux JD
Nature genetics43(3)246-52(2011 Mar)

Role of phosphoinositide-specific phospholipase C ?2 in isolated and syndromic mental retardation.
Lo Vasco VR
European neurology65(5)264-9(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLCH2 might be a putative candidate gene for the neurodevelopmental delay observed in patients bearing 1p36.3 deletions.
Title: Role of phosphoinositide-specific phospholipase C ?2 in isolated and syndromic mental retardation.

The researchers investigated the D1S80 minisatellite and associated single nucelotide polymorphisms in a Tamil population in India.
Title: Investigating SNPs flanking the D1S80 locus in a Tamil population from India.

Purified Gbeta 1gamma 2 stimulated the activity of purified PLC-eta2 in a concentration-dependent manner similar to that observed with purified PLC-beta2.
Title: Activation of human phospholipase C-eta2 by Gbetagamma.

The molecular cloning and sequencing of phospholipase C eta2 are reported.
Title: Molecular cloning and characterization of PLC-eta2.

PLC(eta)2 is a neuron-specific isozyme that may be important for the formation and/or maintenance of the neuronal network in the postnatal brain
Title: A novel phospholipase C, PLC(eta)2, is a neuron-specific isozyme.

The following PLCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu106378	XM_024451062.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu106379	XM_024451063.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu106380	XM_024451064.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu90827	XM_017002873.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu90828	XM_017002874.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu34000	NM_001303012.2 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu63098	NM_001303013.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu106375	XM_024451058.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu106376	XM_024451059.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu106377	XM_024451061.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu106377	XM_024451060.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu90824	XM_017002870.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90825	XM_017002871.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu90826	XM_017002872.1 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu63097	NM_014638.4 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu63087	XM_011542456.2 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu63087	XM_011542455.2 Latest version!	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu63097	NM_014638.3	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu34000	NM_001303012.1	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCH2 ( XM_024451062.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306830.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu106378
Clone ID Related Accession (Same CDS sequence)	XM_024451062.1
Accession Version	XM_024451062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG TGAGGCTGGG CCGTGGCTCC GTCACACCTG TGATGGAAGT 
CTGAGGGGGG AGGGTTGGGG CTACCTGGTG TGCCCGGGTG CCCTGCCCAG GCCTCCCTCA 
GTGACAGTCC TGGGCGCCCT CCCCTCTAGG TGGGCAGTCC CGGAAGCAGC ACCGGGAGTG 
GCACTGGGAG TGGTGTGGGC CGGGGGCTCC AGGCTGGAGC GGTGCTGGGC CGGGCCCTCC 
CCATGGGACA CTCCTCCTGG CGCTGCTTCC CCAGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGG TAAGGCGCCA GCTGCGGTGG CAGAGAAGAG CCCTGTGCGA 
GTGCGGCCCC CGCGTGTCCT GGACGGCCCC GGGCCTGCTG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGATCCTG CGCCGGCGTG AACACCGGGG GCCTGCAGAG GGAGCGGCCA 
CCCAGCCCGG GGCCTGCAAG CAGGCAGGCA GCCATTCGCC AGCAGCCCCG GGCCCGGGCT 
GACTCACTGG GGGCCCCCTG CTGTGGCCTG GACCCTCACG CTATCCCGGG GAGAAGCAGA 
GAGGCCCCCA AGGGTCCTGG GGCCTGGAGG CAGGGTCCAG GCGGTAGCGG CTCCATGTCC 
TCGGACTCCA GCAGCCCAGA CAGCCCGGGC ATCCCCGAAA GGTCCCCCCG CTGGCCTGAG 
GGTGCCTGCA GGCAACCGGG GGCCCTGCAG GGAGAGATGA GTGCCTTGTT TGCTCAAAAG 
CTGGAGGAGA TCAGGAGTAA ATCCCCCATG TTCTCCGCCG TTAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306830.1
CDS	21..4031
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGEAGPWLRHTCDGSLRGEGWGYLVCPGALPRPPSVTVLGALPSRWAVPEAAPGVALGVVWAGGSRLERCWAGPSPWDTPPGAASPGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSAVRN

Target ORF information:

RefSeq Version	XM_024451062.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451062.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG TGAGGCTGGG CCGTGGCTCC GTCACACCTG TGATGGAAGT 
CTGAGGGGGG AGGGTTGGGG CTACCTGGTG TGCCCGGGTG CCCTGCCCAG GCCTCCCTCA 
GTGACAGTCC TGGGCGCCCT CCCCTCTAGG TGGGCAGTCC CGGAAGCAGC ACCGGGAGTG 
GCACTGGGAG TGGTGTGGGC CGGGGGCTCC AGGCTGGAGC GGTGCTGGGC CGGGCCCTCC 
CCATGGGACA CTCCTCCTGG CGCTGCTTCC CCAGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGG TAAGGCGCCA GCTGCGGTGG CAGAGAAGAG CCCTGTGCGA 
GTGCGGCCCC CGCGTGTCCT GGACGGCCCC GGGCCTGCTG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGATCCTG CGCCGGCGTG AACACCGGGG GCCTGCAGAG GGAGCGGCCA 
CCCAGCCCGG GGCCTGCAAG CAGGCAGGCA GCCATTCGCC AGCAGCCCCG GGCCCGGGCT 
GACTCACTGG GGGCCCCCTG CTGTGGCCTG GACCCTCACG CTATCCCGGG GAGAAGCAGA 
GAGGCCCCCA AGGGTCCTGG GGCCTGGAGG CAGGGTCCAG GCGGTAGCGG CTCCATGTCC 
TCGGACTCCA GCAGCCCAGA CAGCCCGGGC ATCCCCGAAA GGTCCCCCCG CTGGCCTGAG 
GGTGCCTGCA GGCAACCGGG GGCCCTGCAG GGAGAGATGA GTGCCTTGTT TGCTCAAAAG 
CTGGAGGAGA TCAGGAGTAA ATCCCCCATG TTCTCCGCCG TTAGGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_024451063.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306831.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X9, mRNA.

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CloneID	OHu106379
Clone ID Related Accession (Same CDS sequence)	XM_024451063.1
Accession Version	XM_024451063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG TGAGGCTGGG CCGTGGCTCC GTCACACCTG TGATGGAAGT 
CTGAGGGGGG AGGGTTGGGG CTACCTGGTG TGCCCGGGTG CCCTGCCCAG GCCTCCCTCA 
GTGACAGTCC TGGGCGCCCT CCCCTCTAGG TGGGCAGTCC CGGAAGCAGC ACCGGGAGTG 
GCACTGGGAG TGGTGTGGGC CGGGGGCTCC AGGCTGGAGC GGTGCTGGGC CGGGCCCTCC 
CCATGGGACA CTCCTCCTGG CGCTGCTTCC CCAGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306831.1
CDS	21..3560
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGEAGPWLRHTCDGSLRGEGWGYLVCPGALPRPPSVTVLGALPSRWAVPEAAPGVALGVVWAGGSRLERCWAGPSPWDTPPGAASPGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRAATPPTVQPGNHR

Target ORF information:

RefSeq Version	XM_024451063.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451063.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG TGAGGCTGGG CCGTGGCTCC GTCACACCTG TGATGGAAGT 
CTGAGGGGGG AGGGTTGGGG CTACCTGGTG TGCCCGGGTG CCCTGCCCAG GCCTCCCTCA 
GTGACAGTCC TGGGCGCCCT CCCCTCTAGG TGGGCAGTCC CGGAAGCAGC ACCGGGAGTG 
GCACTGGGAG TGGTGTGGGC CGGGGGCTCC AGGCTGGAGC GGTGCTGGGC CGGGCCCTCC 
CCATGGGACA CTCCTCCTGG CGCTGCTTCC CCAGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_024451064.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306832.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu106380
Clone ID Related Accession (Same CDS sequence)	XM_024451064.1
Accession Version	XM_024451064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGTTAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306832.1
CDS	21..3275
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRVRN

Target ORF information:

RefSeq Version	XM_024451064.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451064.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGTTAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_017002873.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858362.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.

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CloneID	OHu90827
Clone ID Related Accession (Same CDS sequence)	XM_017002873.1
Accession Version	XM_017002873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GGCGTGTTCA ACCCCAACTC GGAGGACCCC CTGCCCGGGC AGCTCAAGAA GCAGCTGGTG 
CTCCGGATCA TCAGTGGCCA GCAGCTTCCC AAGCCGCGCG ACTCCATGCT GGGGGACCGT 
GGGGAGATCA TCGACCCCTT TGTGGAGGTG GAGATCATTG GGCTCCCTGT GGACTGCAGC 
AGGGAGCAGA CCCGCGTGGT GGACGACAAC GGGTTCAACC CCACCTGGGA GGAGACCCTG 
GTTTTCATGG TGCACATGCC GGAGATCGCG CTGGTCCGCT TCCTCGTCTG GGACCACGAT 
CCCATCGGGC GTGACTTCAT TGGCCAGAGG ACGCTGGCCT TCAGCAGCAT GATGCCAGGC 
TACAGACACG TGTACCTAGA AGGGATGGAA GAGGCCTCCA TCTTCGTGCA TGTGGCTGTC 
AGTGACATCA GCGGTAAGGT CAAGCAGGCT CTGGGCCTAA AAGGCCTCTT CCTCCGAGGC 
CCAAAGCCCG GCTCGCTGGA CAGTCATGCT GCTGGGCGGC CCCCGGCCCG GCCCTCCGTT 
AGCCAGCGGA TCCTGCGGCG CACGGCCAGC GCCCCGACCA AGAGCCAGAA GCCGGGCCGC 
AGGGGCTTCC CGGAGCTGGT CCTGGGTACA CGGGACACAG GCTCCAAGGG GGTGGCAGAC 
GATGTGGTGC CCCCCGGGCC CGGACCTGCT CCGGAAGCCC CAGCCCAGGA GGGGCCCGGC 
AGCGGCAGCC CCCGAGACAC CCGCCCCCTC TCCACGCAGC GGCCACTCCC CCCACTGTGC 
AGCCTGGAAA CCATCGCTGA GGAGCCCGCC CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT 
GTCCCCACCA GCTCTTCTCA GGGACGGCCC CCATACCCCA CAGGACCCGG AGCCAATGTG 
GCAAGCCCCC TAGAGGACAC TGAGGAGCCC CGAGACAGCA GGCCTCGGCC GTGCAACGGC 
GAGGGCGCCG GCGGGGCATA CGAGAGGGCC CCCGGCAGCC AGACGGACGG CAGGAGCCAG 
CCCCGGACCC TGGGCCACCT GCCCGTGATT AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC 
ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC 
TCCGATGCCA CGGGCAGTGA CCCGCTGTGG CAGCGGCTGG AGCCATGTGG CCACCGAGAC 
AGCGTTTCCT CCTCCTCCAG CATGTCATCC AGCGACACTG TCATTGACCT CTCCCTGCCC 
AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC CTCGCTGGAG CCCACATGGG ACGCCTGCCC 
CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC CCAGACCTGC CACCTGTGAC CAAGAGCAAA 
TCCAACCCCA ACCTTCGGGC TACAGGCCAG CGGCCTCCCA TACCTGACGA ACTGCAGCCC 
AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC 
CTGGTGGGCG TGCAGGACTG CCCCGTGGCT GCCAAGTCCA AGAGCCTGGG CGACCTCACT 
GCTGATGACT TTGCCCCTAG CTTTGAGGGC GGCTCCCGCA GACTGAGCCA CAGCCTGGGC 
CTCCCGGGAG GGACACGGCG GGTGTCGGGG CCAGGGGTGA GACGGGACAC CCTGACAGAG 
CAGCTGCGCT GGCTCACTGT CTTCCAGCAG GCAGGAGACA TCACGTCACC CACCAGCCTG 
GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC 
AGCCACAGCC GCGTGCGTGC CATTGCCAGC CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG 
AGACTGCAGG GCCTGGGCCG GCAGGGACCC CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC 
GCCTGCTCCG TGGGCCACGA GGGCAGTGTG GATGCACCAG CACCCTCCAA GGGAGCCCTC 
GGGCCAGCAT CCGCGGCTGC TGAAAACCTG GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858362.1
CDS	21..4448
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRDTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_017002873.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002873.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GGCGTGTTCA ACCCCAACTC GGAGGACCCC CTGCCCGGGC AGCTCAAGAA GCAGCTGGTG 
CTCCGGATCA TCAGTGGCCA GCAGCTTCCC AAGCCGCGCG ACTCCATGCT GGGGGACCGT 
GGGGAGATCA TCGACCCCTT TGTGGAGGTG GAGATCATTG GGCTCCCTGT GGACTGCAGC 
AGGGAGCAGA CCCGCGTGGT GGACGACAAC GGGTTCAACC CCACCTGGGA GGAGACCCTG 
GTTTTCATGG TGCACATGCC GGAGATCGCG CTGGTCCGCT TCCTCGTCTG GGACCACGAT 
CCCATCGGGC GTGACTTCAT TGGCCAGAGG ACGCTGGCCT TCAGCAGCAT GATGCCAGGC 
TACAGACACG TGTACCTAGA AGGGATGGAA GAGGCCTCCA TCTTCGTGCA TGTGGCTGTC 
AGTGACATCA GCGGTAAGGT CAAGCAGGCT CTGGGCCTAA AAGGCCTCTT CCTCCGAGGC 
CCAAAGCCCG GCTCGCTGGA CAGTCATGCT GCTGGGCGGC CCCCGGCCCG GCCCTCCGTT 
AGCCAGCGGA TCCTGCGGCG CACGGCCAGC GCCCCGACCA AGAGCCAGAA GCCGGGCCGC 
AGGGGCTTCC CGGAGCTGGT CCTGGGTACA CGGGACACAG GCTCCAAGGG GGTGGCAGAC 
GATGTGGTGC CCCCCGGGCC CGGACCTGCT CCGGAAGCCC CAGCCCAGGA GGGGCCCGGC 
AGCGGCAGCC CCCGAGACAC CCGCCCCCTC TCCACGCAGC GGCCACTCCC CCCACTGTGC 
AGCCTGGAAA CCATCGCTGA GGAGCCCGCC CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT 
GTCCCCACCA GCTCTTCTCA GGGACGGCCC CCATACCCCA CAGGACCCGG AGCCAATGTG 
GCAAGCCCCC TAGAGGACAC TGAGGAGCCC CGAGACAGCA GGCCTCGGCC GTGCAACGGC 
GAGGGCGCCG GCGGGGCATA CGAGAGGGCC CCCGGCAGCC AGACGGACGG CAGGAGCCAG 
CCCCGGACCC TGGGCCACCT GCCCGTGATT AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC 
ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC 
TCCGATGCCA CGGGCAGTGA CCCGCTGTGG CAGCGGCTGG AGCCATGTGG CCACCGAGAC 
AGCGTTTCCT CCTCCTCCAG CATGTCATCC AGCGACACTG TCATTGACCT CTCCCTGCCC 
AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC CTCGCTGGAG CCCACATGGG ACGCCTGCCC 
CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC CCAGACCTGC CACCTGTGAC CAAGAGCAAA 
TCCAACCCCA ACCTTCGGGC TACAGGCCAG CGGCCTCCCA TACCTGACGA ACTGCAGCCC 
AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC 
CTGGTGGGCG TGCAGGACTG CCCCGTGGCT GCCAAGTCCA AGAGCCTGGG CGACCTCACT 
GCTGATGACT TTGCCCCTAG CTTTGAGGGC GGCTCCCGCA GACTGAGCCA CAGCCTGGGC 
CTCCCGGGAG GGACACGGCG GGTGTCGGGG CCAGGGGTGA GACGGGACAC CCTGACAGAG 
CAGCTGCGCT GGCTCACTGT CTTCCAGCAG GCAGGAGACA TCACGTCACC CACCAGCCTG 
GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC 
AGCCACAGCC GCGTGCGTGC CATTGCCAGC CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG 
AGACTGCAGG GCCTGGGCCG GCAGGGACCC CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC 
GCCTGCTCCG TGGGCCACGA GGGCAGTGTG GATGCACCAG CACCCTCCAA GGGAGCCCTC 
GGGCCAGCAT CCGCGGCTGC TGAAAACCTG GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_017002874.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858363.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X10, mRNA.

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CloneID	OHu90828
Clone ID Related Accession (Same CDS sequence)	XM_017002874.1
Accession Version	XM_017002874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGCGGCCA CTCCCCCCAC TGTGCAGCCT GGAAACCATC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858363.1
CDS	21..3305
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRAATPPTVQPGNHR

Target ORF information:

RefSeq Version	XM_017002874.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002874.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGCGGCCA CTCCCCCCAC TGTGCAGCCT GGAAACCATC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( NM_001303012.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289941.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 2, mRNA.

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CloneID	OHu34000
Clone ID Related Accession (Same CDS sequence)	NM_001303012.1 , NM_001303012.2
Accession Version	NM_001303012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB007919.2 and BM971622.1. On or before Dec 12, 2014 this sequence version replaced XM_006711055.1, XM_006724951.1. Transcript Variant: This variant (2) contains an alternate 5'-terminal exon, uses an alternate start codon, and uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. The encoded isoform (2) has shorter and distinct N- and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB007919.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGAGC CTGGGCCCCC AGGTGGGCTC AGCCAAGACC AAGTGGAGCG GTGCATGGGT 
GCCATGCAAG AGGGGATGCA GATGGTGAAG CTGCGTGGCG GCTCCAAGGG CCTGGTCCGC 
TTCTACTACC TGGACGAGCA CCGCTCCTGC ATCCGCTGGA GGCCCTCACG CAAGAACGAG 
AAGGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGGCGGCA GTCGGAGGTC 
TTCCAGCGCT ACCCTGACGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC 
AGCCACCGCG AGTCGCTGGA CCTGGTCTCC ACCAGCAGCG AGGTGGCGCG CACCTGGGTC 
ACTGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC TCGCCGCCAG 
CGCACCAGGG ACCAGTGGCT GAAGCAGACG TTTGACGAGG CCGACAAGAA CGGGGATGGC 
AGCCTGAGCA TTGGCGAGGT CCTGCAGCTG CTGCACAAGC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAGGGAAGCG GACACGGATG ACCACCAAGG GACGCTGGGT 
TTTGAAGAGT TCTGTGCCTT CTACAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA CAAGGACCAC CTGGATGCCG CCAGCCTGCA GCGCTTCCTG 
CAGGTGGAGC AGAAGATGGC GGGTGTGACC CTCGAGAGCT GCCAGGACAT CATCGAGCAG 
TTTGAGCCAT GCCCAGAAAA CAAGAGTAAG GGGCTGCTGG GCATTGATGG CTTCACCAAC 
TACACCAGGA GCCCTGCTGG TGACATCTTC AACCCTGAGC ACCACCATGT GCACCAGGAC 
ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC ACGGGTGGAC ATGTATGCTT GGGTCCTGCA GGCTGGCTGC 
CGCTGCGTGG AGGTGGACTG CTGGGATGGG CCCGACGGGG AGCCCATTGT GCACCATGGC 
TACACTCTGA CTTCCAAGAT CCTCTTCAAA GACGTCATTG AAACCATCAA CAAATATGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATCGAAA ACCACTGCAG TGTCATCCAG 
CAGAAGAAAA TGGCCCAGTA TCTGACTGAC ATCCTTGGGG ACAAGCTGGA CCTGTCATCA 
GTGAGCAGTG AAGATGCCAC CACACTCCCC TCTCCACAGA TGCTCAAGGG CAAGATCCTC 
GTGAAGGGGA AGAAGCTCCC AGCCAACATC AGCGAGGATG CGGAGGAAGG CGAGGTGTCT 
GATGAGGACA GTGCTGATGA GATTGACGAT GACTGCAAGC TCCTCAATGG GGATGCATCC 
ACCAATCGAA AGCGTGTAGA AAACACTGCT AAGAGGAAAC TGGATTCCCT CATCAAAGAG 
TCGAAGATTC GGGACTGTGA GGACCCCAAC AACTTCTCCG TCTCCACACT GTCCCCATCT 
GGAAAGCTCG GACGCAAGAG CAAGGCTGAA GAGGACGTGG AGTCTGGGGA GGATGCCGGG 
GCCAGCAGAC GCAATGGCCG CCTCGTCGTG GGAAGCTTCT CCAGGCGCAA GAAGAAGGGC 
AGCAAGCTGA AGAAGGCGGC CAGCGTGGAG GAGGGAGATG AGGGTCAGGA CTCCCCGGGA 
GGCCAGAGCC GAGGGGCGAC CCGGCAGAAG AAGACCATGA AGCTGTCCCG GGCCCTCTCT 
GACCTGGTGA AGTACACCAA GTCCGTGGCC ACCCACGACA TAGAGATGGA GGCGGCGTCC 
AGCTGGCAGG TGTCGTCCTT CAGCGAGACC AAGGCCCACC AGATTCTGCA GCAGAAGCCG 
GCGCAGTACC TACGCTTCAA CCAGCAGCAG CTCTCCCGCA TCTACCCCTC CTCCTACCGT 
GTGGACTCCA GCAACTACAA CCCGCAGCCC TTCTGGAACG CCGGCTGCCA AATGGTTGCC 
CTGAACTACC AGTCAGAGGG GCGGATGCTG CAGCTGAACC GAGCCAAGTT CAGCGCCAAC 
GGTGGCTGCG GCTACGTACT CAAGCCTGGG TGCATGTGCC AGGGCGTGTT CAACCCCAAC 
TCGGAGGACC CCCTGCCCGG GCAGCTCAAG AAGCAGCTGG TGCTCCGGAT CATCAGTGGC 
CAGCAGCTTC CCAAGCCGCG CGACTCCATG CTGGGGGACC GTGGGGAGAT CATCGACCCC 
TTTGTGGAGG TGGAGATCAT TGGGCTCCCT GTGGACTGCA GCAGGGAGCA GACCCGCGTG 
GTGGACGACA ACGGGTTCAA CCCCACCTGG GAGGAGACCC TGGTTTTCAT GGTGCACATG 
CCGGAGATCG CGCTGGTCCG CTTCCTCGTC TGGGACCACG ATCCCATCGG GCGTGACTTC 
ATTGGCCAGA GGACGCTGGC CTTCAGCAGC ATGATGCCAG GCTACAGACA CGTGTACCTA 
GAAGGGATGG AAGAGGCCTC CATCTTCGTG CATGTGGCTG TCAGTGACAT CAGCGGTAAG 
GTCAAGCAGG CTCTGGGCCT AAAAGGCCTC TTCCTCCGAG GCCCAAAGCC CGGCTCGCTG 
GACAGTCATG CTGCTGGGCG GCCCCCGGCC CGGCCCTCCG TTAGCCAGCG GATCCTGCGG 
CGCACGGCCA GCGCCCCGAC CAAGAGCCAG AAGCCGGGCC GCAGGGGCTT CCCGGAGCTG 
GTCCTGGGTA CACGGGACAC AGGCTCCAAG GGGGTGGCAG ACGATGTGGT GCCCCCCGGG 
CCCGGACCTG CTCCGGAAGC CCCAGCCCAG GAGGGGCCCG GCAGCGGCAG CCCCCGAGGT 
AAGGCGCCAG CTGCGGTGGC AGAGAAGAGC CCTGTGCGAG TGCGGCCCCC GCGTGTCCTG 
GACGGCCCCG GGCCTGCTGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGATCCTGC 
GCCGGCGTGA ACACCGGGGG CCTGCAGAGG GAGCGGCCAC CCAGCCCGGG GCCTGCAAGC 
AGGCAGGCAG CCATTCGCCA GCAGCCCCGG GCCCGGGCTG ACTCACTGGG GGCCCCCTGC 
TGTGGCCTGG ACCCTCACGC TATCCCGGGG AGAAGCAGAG AGGCCCCCAA GGGTCCTGGG 
GCCTGGAGGC AGGGTCCAGG CGGTAGCGGC TCCATGTCCT CGGACTCCAG CAGCCCAGAC 
AGCCCGGGCA TCCCCGAAAG GTCCCCCCGC TGGCCTGAGG GTGCCTGCAG GCAACCGGGG 
GCCCTGCAGG GAGAGATGAG TGCCTTGTTT GCTCAAAAGC TGGAGGAGAT CAGGAGTAAA 
TCCCCCATGT TCTCCGCCGT TAGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289941.1
CDS	162..3551
Translation	MEEPGPPGGLSQDQVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSAVRN

Target ORF information:

RefSeq Version	NM_001303012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303012.1

ORF Insert Sequence:

ATGGAAGAGC CTGGGCCCCC AGGTGGGCTC AGCCAAGACC AAGTGGAGCG GTGCATGGGT 
GCCATGCAAG AGGGGATGCA GATGGTGAAG CTGCGTGGCG GCTCCAAGGG CCTGGTCCGC 
TTCTACTACC TGGACGAGCA CCGCTCCTGC ATCCGCTGGA GGCCCTCACG CAAGAACGAG 
AAGGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGGCGGCA GTCGGAGGTC 
TTCCAGCGCT ACCCTGACGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC 
AGCCACCGCG AGTCGCTGGA CCTGGTCTCC ACCAGCAGCG AGGTGGCGCG CACCTGGGTC 
ACTGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC TCGCCGCCAG 
CGCACCAGGG ACCAGTGGCT GAAGCAGACG TTTGACGAGG CCGACAAGAA CGGGGATGGC 
AGCCTGAGCA TTGGCGAGGT CCTGCAGCTG CTGCACAAGC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAGGGAAGCG GACACGGATG ACCACCAAGG GACGCTGGGT 
TTTGAAGAGT TCTGTGCCTT CTACAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA CAAGGACCAC CTGGATGCCG CCAGCCTGCA GCGCTTCCTG 
CAGGTGGAGC AGAAGATGGC GGGTGTGACC CTCGAGAGCT GCCAGGACAT CATCGAGCAG 
TTTGAGCCAT GCCCAGAAAA CAAGAGTAAG GGGCTGCTGG GCATTGATGG CTTCACCAAC 
TACACCAGGA GCCCTGCTGG TGACATCTTC AACCCTGAGC ACCACCATGT GCACCAGGAC 
ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC ACGGGTGGAC ATGTATGCTT GGGTCCTGCA GGCTGGCTGC 
CGCTGCGTGG AGGTGGACTG CTGGGATGGG CCCGACGGGG AGCCCATTGT GCACCATGGC 
TACACTCTGA CTTCCAAGAT CCTCTTCAAA GACGTCATTG AAACCATCAA CAAATATGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATCGAAA ACCACTGCAG TGTCATCCAG 
CAGAAGAAAA TGGCCCAGTA TCTGACTGAC ATCCTTGGGG ACAAGCTGGA CCTGTCATCA 
GTGAGCAGTG AAGATGCCAC CACACTCCCC TCTCCACAGA TGCTCAAGGG CAAGATCCTC 
GTGAAGGGGA AGAAGCTCCC AGCCAACATC AGCGAGGATG CGGAGGAAGG CGAGGTGTCT 
GATGAGGACA GTGCTGATGA GATTGACGAT GACTGCAAGC TCCTCAATGG GGATGCATCC 
ACCAATCGAA AGCGTGTAGA AAACACTGCT AAGAGGAAAC TGGATTCCCT CATCAAAGAG 
TCGAAGATTC GGGACTGTGA GGACCCCAAC AACTTCTCCG TCTCCACACT GTCCCCATCT 
GGAAAGCTCG GACGCAAGAG CAAGGCTGAA GAGGACGTGG AGTCTGGGGA GGATGCCGGG 
GCCAGCAGAC GCAATGGCCG CCTCGTCGTG GGAAGCTTCT CCAGGCGCAA GAAGAAGGGC 
AGCAAGCTGA AGAAGGCGGC CAGCGTGGAG GAGGGAGATG AGGGTCAGGA CTCCCCGGGA 
GGCCAGAGCC GAGGGGCGAC CCGGCAGAAG AAGACCATGA AGCTGTCCCG GGCCCTCTCT 
GACCTGGTGA AGTACACCAA GTCCGTGGCC ACCCACGACA TAGAGATGGA GGCGGCGTCC 
AGCTGGCAGG TGTCGTCCTT CAGCGAGACC AAGGCCCACC AGATTCTGCA GCAGAAGCCG 
GCGCAGTACC TACGCTTCAA CCAGCAGCAG CTCTCCCGCA TCTACCCCTC CTCCTACCGT 
GTGGACTCCA GCAACTACAA CCCGCAGCCC TTCTGGAACG CCGGCTGCCA AATGGTTGCC 
CTGAACTACC AGTCAGAGGG GCGGATGCTG CAGCTGAACC GAGCCAAGTT CAGCGCCAAC 
GGTGGCTGCG GCTACGTACT CAAGCCTGGG TGCATGTGCC AGGGCGTGTT CAACCCCAAC 
TCGGAGGACC CCCTGCCCGG GCAGCTCAAG AAGCAGCTGG TGCTCCGGAT CATCAGTGGC 
CAGCAGCTTC CCAAGCCGCG CGACTCCATG CTGGGGGACC GTGGGGAGAT CATCGACCCC 
TTTGTGGAGG TGGAGATCAT TGGGCTCCCT GTGGACTGCA GCAGGGAGCA GACCCGCGTG 
GTGGACGACA ACGGGTTCAA CCCCACCTGG GAGGAGACCC TGGTTTTCAT GGTGCACATG 
CCGGAGATCG CGCTGGTCCG CTTCCTCGTC TGGGACCACG ATCCCATCGG GCGTGACTTC 
ATTGGCCAGA GGACGCTGGC CTTCAGCAGC ATGATGCCAG GCTACAGACA CGTGTACCTA 
GAAGGGATGG AAGAGGCCTC CATCTTCGTG CATGTGGCTG TCAGTGACAT CAGCGGTAAG 
GTCAAGCAGG CTCTGGGCCT AAAAGGCCTC TTCCTCCGAG GCCCAAAGCC CGGCTCGCTG 
GACAGTCATG CTGCTGGGCG GCCCCCGGCC CGGCCCTCCG TTAGCCAGCG GATCCTGCGG 
CGCACGGCCA GCGCCCCGAC CAAGAGCCAG AAGCCGGGCC GCAGGGGCTT CCCGGAGCTG 
GTCCTGGGTA CACGGGACAC AGGCTCCAAG GGGGTGGCAG ACGATGTGGT GCCCCCCGGG 
CCCGGACCTG CTCCGGAAGC CCCAGCCCAG GAGGGGCCCG GCAGCGGCAG CCCCCGAGGT 
AAGGCGCCAG CTGCGGTGGC AGAGAAGAGC CCTGTGCGAG TGCGGCCCCC GCGTGTCCTG 
GACGGCCCCG GGCCTGCTGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGATCCTGC 
GCCGGCGTGA ACACCGGGGG CCTGCAGAGG GAGCGGCCAC CCAGCCCGGG GCCTGCAAGC 
AGGCAGGCAG CCATTCGCCA GCAGCCCCGG GCCCGGGCTG ACTCACTGGG GGCCCCCTGC 
TGTGGCCTGG ACCCTCACGC TATCCCGGGG AGAAGCAGAG AGGCCCCCAA GGGTCCTGGG 
GCCTGGAGGC AGGGTCCAGG CGGTAGCGGC TCCATGTCCT CGGACTCCAG CAGCCCAGAC 
AGCCCGGGCA TCCCCGAAAG GTCCCCCCGC TGGCCTGAGG GTGCCTGCAG GCAACCGGGG 
GCCCTGCAGG GAGAGATGAG TGCCTTGTTT GCTCAAAAGC TGGAGGAGAT CAGGAGTAAA 
TCCCCCATGT TCTCCGCCGT TAGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( NM_001303012.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289941.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 2, mRNA.

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CloneID	OHu34000
Clone ID Related Accession (Same CDS sequence)	NM_001303012.1 , NM_001303012.2
Accession Version	NM_001303012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL139246.21, AB007919.2 and BM971622.1. On May 31, 2019 this sequence version replaced NM_001303012.1. Transcript Variant: This variant (2) contains an alternate 5'-terminal exon, uses an alternate start codon, and uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. The encoded isoform (2) has shorter and distinct N- and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB007919.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGAGC CTGGGCCCCC AGGTGGGCTC AGCCAAGACC AAGTGGAGCG GTGCATGGGT 
GCCATGCAAG AGGGGATGCA GATGGTGAAG CTGCGTGGCG GCTCCAAGGG CCTGGTCCGC 
TTCTACTACC TGGACGAGCA CCGCTCCTGC ATCCGCTGGA GGCCCTCACG CAAGAACGAG 
AAGGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGGCGGCA GTCGGAGGTC 
TTCCAGCGCT ACCCTGACGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC 
AGCCACCGCG AGTCGCTGGA CCTGGTCTCC ACCAGCAGCG AGGTGGCGCG CACCTGGGTC 
ACTGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC TCGCCGCCAG 
CGCACCAGGG ACCAGTGGCT GAAGCAGACG TTTGACGAGG CCGACAAGAA CGGGGATGGC 
AGCCTGAGCA TTGGCGAGGT CCTGCAGCTG CTGCACAAGC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAGGGAAGCG GACACGGATG ACCACCAAGG GACGCTGGGT 
TTTGAAGAGT TCTGTGCCTT CTACAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA CAAGGACCAC CTGGATGCCG CCAGCCTGCA GCGCTTCCTG 
CAGGTGGAGC AGAAGATGGC GGGTGTGACC CTCGAGAGCT GCCAGGACAT CATCGAGCAG 
TTTGAGCCAT GCCCAGAAAA CAAGAGTAAG GGGCTGCTGG GCATTGATGG CTTCACCAAC 
TACACCAGGA GCCCTGCTGG TGACATCTTC AACCCTGAGC ACCACCATGT GCACCAGGAC 
ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC ACGGGTGGAC ATGTATGCTT GGGTCCTGCA GGCTGGCTGC 
CGCTGCGTGG AGGTGGACTG CTGGGATGGG CCCGACGGGG AGCCCATTGT GCACCATGGC 
TACACTCTGA CTTCCAAGAT CCTCTTCAAA GACGTCATTG AAACCATCAA CAAATATGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATCGAAA ACCACTGCAG TGTCATCCAG 
CAGAAGAAAA TGGCCCAGTA TCTGACTGAC ATCCTTGGGG ACAAGCTGGA CCTGTCATCA 
GTGAGCAGTG AAGATGCCAC CACACTCCCC TCTCCACAGA TGCTCAAGGG CAAGATCCTC 
GTGAAGGGGA AGAAGCTCCC AGCCAACATC AGCGAGGATG CGGAGGAAGG CGAGGTGTCT 
GATGAGGACA GTGCTGATGA GATTGACGAT GACTGCAAGC TCCTCAATGG GGATGCATCC 
ACCAATCGAA AGCGTGTAGA AAACACTGCT AAGAGGAAAC TGGATTCCCT CATCAAAGAG 
TCGAAGATTC GGGACTGTGA GGACCCCAAC AACTTCTCCG TCTCCACACT GTCCCCATCT 
GGAAAGCTCG GACGCAAGAG CAAGGCTGAA GAGGACGTGG AGTCTGGGGA GGATGCCGGG 
GCCAGCAGAC GCAATGGCCG CCTCGTCGTG GGAAGCTTCT CCAGGCGCAA GAAGAAGGGC 
AGCAAGCTGA AGAAGGCGGC CAGCGTGGAG GAGGGAGATG AGGGTCAGGA CTCCCCGGGA 
GGCCAGAGCC GAGGGGCGAC CCGGCAGAAG AAGACCATGA AGCTGTCCCG GGCCCTCTCT 
GACCTGGTGA AGTACACCAA GTCCGTGGCC ACCCACGACA TAGAGATGGA GGCGGCGTCC 
AGCTGGCAGG TGTCGTCCTT CAGCGAGACC AAGGCCCACC AGATTCTGCA GCAGAAGCCG 
GCGCAGTACC TACGCTTCAA CCAGCAGCAG CTCTCCCGCA TCTACCCCTC CTCCTACCGT 
GTGGACTCCA GCAACTACAA CCCGCAGCCC TTCTGGAACG CCGGCTGCCA AATGGTTGCC 
CTGAACTACC AGTCAGAGGG GCGGATGCTG CAGCTGAACC GAGCCAAGTT CAGCGCCAAC 
GGTGGCTGCG GCTACGTACT CAAGCCTGGG TGCATGTGCC AGGGCGTGTT CAACCCCAAC 
TCGGAGGACC CCCTGCCCGG GCAGCTCAAG AAGCAGCTGG TGCTCCGGAT CATCAGTGGC 
CAGCAGCTTC CCAAGCCGCG CGACTCCATG CTGGGGGACC GTGGGGAGAT CATCGACCCC 
TTTGTGGAGG TGGAGATCAT TGGGCTCCCT GTGGACTGCA GCAGGGAGCA GACCCGCGTG 
GTGGACGACA ACGGGTTCAA CCCCACCTGG GAGGAGACCC TGGTTTTCAT GGTGCACATG 
CCGGAGATCG CGCTGGTCCG CTTCCTCGTC TGGGACCACG ATCCCATCGG GCGTGACTTC 
ATTGGCCAGA GGACGCTGGC CTTCAGCAGC ATGATGCCAG GCTACAGACA CGTGTACCTA 
GAAGGGATGG AAGAGGCCTC CATCTTCGTG CATGTGGCTG TCAGTGACAT CAGCGGTAAG 
GTCAAGCAGG CTCTGGGCCT AAAAGGCCTC TTCCTCCGAG GCCCAAAGCC CGGCTCGCTG 
GACAGTCATG CTGCTGGGCG GCCCCCGGCC CGGCCCTCCG TTAGCCAGCG GATCCTGCGG 
CGCACGGCCA GCGCCCCGAC CAAGAGCCAG AAGCCGGGCC GCAGGGGCTT CCCGGAGCTG 
GTCCTGGGTA CACGGGACAC AGGCTCCAAG GGGGTGGCAG ACGATGTGGT GCCCCCCGGG 
CCCGGACCTG CTCCGGAAGC CCCAGCCCAG GAGGGGCCCG GCAGCGGCAG CCCCCGAGGT 
AAGGCGCCAG CTGCGGTGGC AGAGAAGAGC CCTGTGCGAG TGCGGCCCCC GCGTGTCCTG 
GACGGCCCCG GGCCTGCTGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGATCCTGC 
GCCGGCGTGA ACACCGGGGG CCTGCAGAGG GAGCGGCCAC CCAGCCCGGG GCCTGCAAGC 
AGGCAGGCAG CCATTCGCCA GCAGCCCCGG GCCCGGGCTG ACTCACTGGG GGCCCCCTGC 
TGTGGCCTGG ACCCTCACGC TATCCCGGGG AGAAGCAGAG AGGCCCCCAA GGGTCCTGGG 
GCCTGGAGGC AGGGTCCAGG CGGTAGCGGC TCCATGTCCT CGGACTCCAG CAGCCCAGAC 
AGCCCGGGCA TCCCCGAAAG GTCCCCCCGC TGGCCTGAGG GTGCCTGCAG GCAACCGGGG 
GCCCTGCAGG GAGAGATGAG TGCCTTGTTT GCTCAAAAGC TGGAGGAGAT CAGGAGTAAA 
TCCCCCATGT TCTCCGCCGT TAGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289941.1
CDS	170..3559
Translation	MEEPGPPGGLSQDQVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSAVRN

Target ORF information:

RefSeq Version	NM_001303012.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303012.2

ORF Insert Sequence:

ATGGAAGAGC CTGGGCCCCC AGGTGGGCTC AGCCAAGACC AAGTGGAGCG GTGCATGGGT 
GCCATGCAAG AGGGGATGCA GATGGTGAAG CTGCGTGGCG GCTCCAAGGG CCTGGTCCGC 
TTCTACTACC TGGACGAGCA CCGCTCCTGC ATCCGCTGGA GGCCCTCACG CAAGAACGAG 
AAGGCCAAGA TCTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGGCGGCA GTCGGAGGTC 
TTCCAGCGCT ACCCTGACGG CAGCTTCGAC CCCAACTGCT GCTTCAGCAT CTACCACGGC 
AGCCACCGCG AGTCGCTGGA CCTGGTCTCC ACCAGCAGCG AGGTGGCGCG CACCTGGGTC 
ACTGGCCTGC GCTACCTCAT GGCCGGCATC AGCGACGAGG ACAGCCTGGC TCGCCGCCAG 
CGCACCAGGG ACCAGTGGCT GAAGCAGACG TTTGACGAGG CCGACAAGAA CGGGGATGGC 
AGCCTGAGCA TTGGCGAGGT CCTGCAGCTG CTGCACAAGC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AGCAGATGTT CAGGGAAGCG GACACGGATG ACCACCAAGG GACGCTGGGT 
TTTGAAGAGT TCTGTGCCTT CTACAAGATG ATGTCCACCC GCCGGGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA CAAGGACCAC CTGGATGCCG CCAGCCTGCA GCGCTTCCTG 
CAGGTGGAGC AGAAGATGGC GGGTGTGACC CTCGAGAGCT GCCAGGACAT CATCGAGCAG 
TTTGAGCCAT GCCCAGAAAA CAAGAGTAAG GGGCTGCTGG GCATTGATGG CTTCACCAAC 
TACACCAGGA GCCCTGCTGG TGACATCTTC AACCCTGAGC ACCACCATGT GCACCAGGAC 
ATGACGCAGC CGCTGAGCCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC ACGGGTGGAC ATGTATGCTT GGGTCCTGCA GGCTGGCTGC 
CGCTGCGTGG AGGTGGACTG CTGGGATGGG CCCGACGGGG AGCCCATTGT GCACCATGGC 
TACACTCTGA CTTCCAAGAT CCTCTTCAAA GACGTCATTG AAACCATCAA CAAATATGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATCGAAA ACCACTGCAG TGTCATCCAG 
CAGAAGAAAA TGGCCCAGTA TCTGACTGAC ATCCTTGGGG ACAAGCTGGA CCTGTCATCA 
GTGAGCAGTG AAGATGCCAC CACACTCCCC TCTCCACAGA TGCTCAAGGG CAAGATCCTC 
GTGAAGGGGA AGAAGCTCCC AGCCAACATC AGCGAGGATG CGGAGGAAGG CGAGGTGTCT 
GATGAGGACA GTGCTGATGA GATTGACGAT GACTGCAAGC TCCTCAATGG GGATGCATCC 
ACCAATCGAA AGCGTGTAGA AAACACTGCT AAGAGGAAAC TGGATTCCCT CATCAAAGAG 
TCGAAGATTC GGGACTGTGA GGACCCCAAC AACTTCTCCG TCTCCACACT GTCCCCATCT 
GGAAAGCTCG GACGCAAGAG CAAGGCTGAA GAGGACGTGG AGTCTGGGGA GGATGCCGGG 
GCCAGCAGAC GCAATGGCCG CCTCGTCGTG GGAAGCTTCT CCAGGCGCAA GAAGAAGGGC 
AGCAAGCTGA AGAAGGCGGC CAGCGTGGAG GAGGGAGATG AGGGTCAGGA CTCCCCGGGA 
GGCCAGAGCC GAGGGGCGAC CCGGCAGAAG AAGACCATGA AGCTGTCCCG GGCCCTCTCT 
GACCTGGTGA AGTACACCAA GTCCGTGGCC ACCCACGACA TAGAGATGGA GGCGGCGTCC 
AGCTGGCAGG TGTCGTCCTT CAGCGAGACC AAGGCCCACC AGATTCTGCA GCAGAAGCCG 
GCGCAGTACC TACGCTTCAA CCAGCAGCAG CTCTCCCGCA TCTACCCCTC CTCCTACCGT 
GTGGACTCCA GCAACTACAA CCCGCAGCCC TTCTGGAACG CCGGCTGCCA AATGGTTGCC 
CTGAACTACC AGTCAGAGGG GCGGATGCTG CAGCTGAACC GAGCCAAGTT CAGCGCCAAC 
GGTGGCTGCG GCTACGTACT CAAGCCTGGG TGCATGTGCC AGGGCGTGTT CAACCCCAAC 
TCGGAGGACC CCCTGCCCGG GCAGCTCAAG AAGCAGCTGG TGCTCCGGAT CATCAGTGGC 
CAGCAGCTTC CCAAGCCGCG CGACTCCATG CTGGGGGACC GTGGGGAGAT CATCGACCCC 
TTTGTGGAGG TGGAGATCAT TGGGCTCCCT GTGGACTGCA GCAGGGAGCA GACCCGCGTG 
GTGGACGACA ACGGGTTCAA CCCCACCTGG GAGGAGACCC TGGTTTTCAT GGTGCACATG 
CCGGAGATCG CGCTGGTCCG CTTCCTCGTC TGGGACCACG ATCCCATCGG GCGTGACTTC 
ATTGGCCAGA GGACGCTGGC CTTCAGCAGC ATGATGCCAG GCTACAGACA CGTGTACCTA 
GAAGGGATGG AAGAGGCCTC CATCTTCGTG CATGTGGCTG TCAGTGACAT CAGCGGTAAG 
GTCAAGCAGG CTCTGGGCCT AAAAGGCCTC TTCCTCCGAG GCCCAAAGCC CGGCTCGCTG 
GACAGTCATG CTGCTGGGCG GCCCCCGGCC CGGCCCTCCG TTAGCCAGCG GATCCTGCGG 
CGCACGGCCA GCGCCCCGAC CAAGAGCCAG AAGCCGGGCC GCAGGGGCTT CCCGGAGCTG 
GTCCTGGGTA CACGGGACAC AGGCTCCAAG GGGGTGGCAG ACGATGTGGT GCCCCCCGGG 
CCCGGACCTG CTCCGGAAGC CCCAGCCCAG GAGGGGCCCG GCAGCGGCAG CCCCCGAGGT 
AAGGCGCCAG CTGCGGTGGC AGAGAAGAGC CCTGTGCGAG TGCGGCCCCC GCGTGTCCTG 
GACGGCCCCG GGCCTGCTGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGATCCTGC 
GCCGGCGTGA ACACCGGGGG CCTGCAGAGG GAGCGGCCAC CCAGCCCGGG GCCTGCAAGC 
AGGCAGGCAG CCATTCGCCA GCAGCCCCGG GCCCGGGCTG ACTCACTGGG GGCCCCCTGC 
TGTGGCCTGG ACCCTCACGC TATCCCGGGG AGAAGCAGAG AGGCCCCCAA GGGTCCTGGG 
GCCTGGAGGC AGGGTCCAGG CGGTAGCGGC TCCATGTCCT CGGACTCCAG CAGCCCAGAC 
AGCCCGGGCA TCCCCGAAAG GTCCCCCCGC TGGCCTGAGG GTGCCTGCAG GCAACCGGGG 
GCCCTGCAGG GAGAGATGAG TGCCTTGTTT GCTCAAAAGC TGGAGGAGAT CAGGAGTAAA 
TCCCCCATGT TCTCCGCCGT TAGGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( NM_001303013.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289942.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 3, mRNA.

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CloneID	OHu63098
Clone ID Related Accession (Same CDS sequence)	NM_001303013.1
Accession Version	NM_001303013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ176850.1, BC144620.1, AL139246.21, BC043358.1 and BM971622.1. Transcript Variant: This variant (3) differs in its 5' UTR, uses an alternate start codon, and uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. The encoded isoform (3) has shorter and distinct N- and C-termini, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC144620.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGTACCTC TGGGACCCGG GCTTTCTGTG AGCAGGTGTG CAGGAACCGA GGGGGTGAGA 
CCAGTCTGTT CCTGGGCTGG GACAGCTGAC TCTGGCCTGG ACTCCCTGGA CCCTGCAGCC 
CCCTCTCCTG GGACAAGCTA CAGCAGGAGG CTGAATGGAG AGAGGAGGGG AGAGAAGGAG 
CGAGTGGAGC GGTGCATGGG TGCCATGCAA GAGGGGATGC AGATGGTGAA GCTGCGTGGC 
GGCTCCAAGG GCCTGGTCCG CTTCTACTAC CTGGACGAGC ACCGCTCCTG CATCCGCTGG 
AGGCCCTCAC GCAAGAACGA GAAGGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTGAGT 
GAGGGGCGGC AGTCGGAGGT CTTCCAGCGC TACCCTGACG GCAGCTTCGA CCCCAACTGC 
TGCTTCAGCA TCTACCACGG CAGCCACCGC GAGTCGCTGG ACCTGGTCTC CACCAGCAGC 
GAGGTGGCGC GCACCTGGGT CACTGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG 
GACAGCCTGG CTCGCCGCCA GCGCACCAGG GACCAGTGGC TGAAGCAGAC GTTTGACGAG 
GCCGACAAGA ACGGGGATGG CAGCCTGAGC ATTGGCGAGG TCCTGCAGCT GCTGCACAAG 
CTCAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAGGGAAGC GGACACGGAT 
GACCACCAAG GGACGCTGGG TTTTGAAGAG TTCTGTGCCT TCTACAAGAT GATGTCCACC 
CGCCGGGACC TCTACCTGCT CATGCTGACC TACAGCAACC ACAAGGACCA CCTGGATGCC 
GCCAGCCTGC AGCGCTTCCT GCAGGTGGAG CAGAAGATGG CGGGTGTGAC CCTCGAGAGC 
TGCCAGGACA TCATCGAGCA GTTTGAGCCA TGCCCAGAAA ACAAGAGTAA GGGGCTGCTG 
GGCATTGATG GCTTCACCAA CTACACCAGG AGCCCTGCTG GTGACATCTT CAACCCTGAG 
CACCACCATG TGCACCAGGA CATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCC 
CACAACACCT ACCTCGTGGG TGACCAGCTC ATGTCCCAGT CACGGGTGGA CATGTATGCT 
TGGGTCCTGC AGGCTGGCTG CCGCTGCGTG GAGGTGGACT GCTGGGATGG GCCCGACGGG 
GAGCCCATTG TGCACCATGG CTACACTCTG ACTTCCAAGA TCCTCTTCAA AGACGTCATT 
GAAACCATCA ACAAATATGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATCGAA 
AACCACTGCA GTGTCATCCA GCAGAAGAAA ATGGCCCAGT ATCTGACTGA CATCCTTGGG 
GACAAGCTGG ACCTGTCATC AGTGAGCAGT GAAGATGCCA CCACACTCCC CTCTCCACAG 
ATGCTCAAGG GCAAGATCCT CGTGAAGGGG AAGAAGCTCC CAGCCAACAT CAGCGAGGAT 
GCGGAGGAAG GCGAGGTGTC TGATGAGGAC AGTGCTGATG AGATTGACGA TGACTGCAAG 
CTCCTCAATG GGGATGCATC CACCAATCGA AAGCGTGTAG AAAACACTGC TAAGAGGAAA 
CTGGATTCCC TCATCAAAGA GTCGAAGATT CGGGACTGTG AGGACCCCAA CAACTTCTCC 
GTCTCCACAC TGTCCCCATC TGGAAAGCTC GGACGCAAGA GCAAGGCTGA AGAGGACGTG 
GAGTCTGGGG AGGATGCCGG GGCCAGCAGA CGCAATGGCC GCCTCGTCGT GGGAAGCTTC 
TCCAGGCGCA AGAAGAAGGG CAGCAAGCTG AAGAAGGCGG CCAGCGTGGA GGAGGGAGAT 
GAGGGTCAGG ACTCCCCGGG AGGCCAGAGC CGAGGGGCGA CCCGGCAGAA GAAGACCATG 
AAGCTGTCCC GGGCCCTCTC TGACCTGGTG AAGTACACCA AGTCCGTGGC CACCCACGAC 
ATAGAGATGG AGGCGGCGTC CAGCTGGCAG GTGTCGTCCT TCAGCGAGAC CAAGGCCCAC 
CAGATTCTGC AGCAGAAGCC GGCGCAGTAC CTACGCTTCA ACCAGCAGCA GCTCTCCCGC 
ATCTACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ACCCGCAGCC CTTCTGGAAC 
GCCGGCTGCC AAATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT GCAGCTGAAC 
CGAGCCAAGT TCAGCGCCAA CGGTGGCTGC GGCTACGTAC TCAAGCCTGG GTGCATGTGC 
CAGGGCGTGT TCAACCCCAA CTCGGAGGAC CCCCTGCCCG GGCAGCTCAA GAAGCAGCTG 
GTGCTCCGGA TCATCAGTGG CCAGCAGCTT CCCAAGCCGC GCGACTCCAT GCTGGGGGAC 
CGTGGGGAGA TCATCGACCC CTTTGTGGAG GTGGAGATCA TTGGGCTCCC TGTGGACTGC 
AGCAGGGAGC AGACCCGCGT GGTGGACGAC AACGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289942.1
CDS	1207..4266
Translation	MVPLGPGLSVSRCAGTEGVRPVCSWAGTADSGLDSLDPAAPSPGTSYSRRLNGERRGEKERVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRAATPPTVQPGNHR

Target ORF information:

RefSeq Version	NM_001303013.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303013.1

ORF Insert Sequence:

ATGGTACCTC TGGGACCCGG GCTTTCTGTG AGCAGGTGTG CAGGAACCGA GGGGGTGAGA 
CCAGTCTGTT CCTGGGCTGG GACAGCTGAC TCTGGCCTGG ACTCCCTGGA CCCTGCAGCC 
CCCTCTCCTG GGACAAGCTA CAGCAGGAGG CTGAATGGAG AGAGGAGGGG AGAGAAGGAG 
CGAGTGGAGC GGTGCATGGG TGCCATGCAA GAGGGGATGC AGATGGTGAA GCTGCGTGGC 
GGCTCCAAGG GCCTGGTCCG CTTCTACTAC CTGGACGAGC ACCGCTCCTG CATCCGCTGG 
AGGCCCTCAC GCAAGAACGA GAAGGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTGAGT 
GAGGGGCGGC AGTCGGAGGT CTTCCAGCGC TACCCTGACG GCAGCTTCGA CCCCAACTGC 
TGCTTCAGCA TCTACCACGG CAGCCACCGC GAGTCGCTGG ACCTGGTCTC CACCAGCAGC 
GAGGTGGCGC GCACCTGGGT CACTGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG 
GACAGCCTGG CTCGCCGCCA GCGCACCAGG GACCAGTGGC TGAAGCAGAC GTTTGACGAG 
GCCGACAAGA ACGGGGATGG CAGCCTGAGC ATTGGCGAGG TCCTGCAGCT GCTGCACAAG 
CTCAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAGGGAAGC GGACACGGAT 
GACCACCAAG GGACGCTGGG TTTTGAAGAG TTCTGTGCCT TCTACAAGAT GATGTCCACC 
CGCCGGGACC TCTACCTGCT CATGCTGACC TACAGCAACC ACAAGGACCA CCTGGATGCC 
GCCAGCCTGC AGCGCTTCCT GCAGGTGGAG CAGAAGATGG CGGGTGTGAC CCTCGAGAGC 
TGCCAGGACA TCATCGAGCA GTTTGAGCCA TGCCCAGAAA ACAAGAGTAA GGGGCTGCTG 
GGCATTGATG GCTTCACCAA CTACACCAGG AGCCCTGCTG GTGACATCTT CAACCCTGAG 
CACCACCATG TGCACCAGGA CATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCC 
CACAACACCT ACCTCGTGGG TGACCAGCTC ATGTCCCAGT CACGGGTGGA CATGTATGCT 
TGGGTCCTGC AGGCTGGCTG CCGCTGCGTG GAGGTGGACT GCTGGGATGG GCCCGACGGG 
GAGCCCATTG TGCACCATGG CTACACTCTG ACTTCCAAGA TCCTCTTCAA AGACGTCATT 
GAAACCATCA ACAAATATGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATCGAA 
AACCACTGCA GTGTCATCCA GCAGAAGAAA ATGGCCCAGT ATCTGACTGA CATCCTTGGG 
GACAAGCTGG ACCTGTCATC AGTGAGCAGT GAAGATGCCA CCACACTCCC CTCTCCACAG 
ATGCTCAAGG GCAAGATCCT CGTGAAGGGG AAGAAGCTCC CAGCCAACAT CAGCGAGGAT 
GCGGAGGAAG GCGAGGTGTC TGATGAGGAC AGTGCTGATG AGATTGACGA TGACTGCAAG 
CTCCTCAATG GGGATGCATC CACCAATCGA AAGCGTGTAG AAAACACTGC TAAGAGGAAA 
CTGGATTCCC TCATCAAAGA GTCGAAGATT CGGGACTGTG AGGACCCCAA CAACTTCTCC 
GTCTCCACAC TGTCCCCATC TGGAAAGCTC GGACGCAAGA GCAAGGCTGA AGAGGACGTG 
GAGTCTGGGG AGGATGCCGG GGCCAGCAGA CGCAATGGCC GCCTCGTCGT GGGAAGCTTC 
TCCAGGCGCA AGAAGAAGGG CAGCAAGCTG AAGAAGGCGG CCAGCGTGGA GGAGGGAGAT 
GAGGGTCAGG ACTCCCCGGG AGGCCAGAGC CGAGGGGCGA CCCGGCAGAA GAAGACCATG 
AAGCTGTCCC GGGCCCTCTC TGACCTGGTG AAGTACACCA AGTCCGTGGC CACCCACGAC 
ATAGAGATGG AGGCGGCGTC CAGCTGGCAG GTGTCGTCCT TCAGCGAGAC CAAGGCCCAC 
CAGATTCTGC AGCAGAAGCC GGCGCAGTAC CTACGCTTCA ACCAGCAGCA GCTCTCCCGC 
ATCTACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ACCCGCAGCC CTTCTGGAAC 
GCCGGCTGCC AAATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT GCAGCTGAAC 
CGAGCCAAGT TCAGCGCCAA CGGTGGCTGC GGCTACGTAC TCAAGCCTGG GTGCATGTGC 
CAGGGCGTGT TCAACCCCAA CTCGGAGGAC CCCCTGCCCG GGCAGCTCAA GAAGCAGCTG 
GTGCTCCGGA TCATCAGTGG CCAGCAGCTT CCCAAGCCGC GCGACTCCAT GCTGGGGGAC 
CGTGGGGAGA TCATCGACCC CTTTGTGGAG GTGGAGATCA TTGGGCTCCC TGTGGACTGC 
AGCAGGGAGC AGACCCGCGT GGTGGACGAC AACGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_024451058.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306826.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

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CloneID	OHu106375
Clone ID Related Accession (Same CDS sequence)	XM_024451058.1
Accession Version	XM_024451058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGG AGCAGGCCGG GGCTTGTGGG GGCCTCCGGT ACCTCTGCTG GGAGCTCGCG 
ATCCAAGGGA CACAGCGTGA GGGGCCACCG CAGGTGTGTG ATGCGCTGCT GGCCCAGGCT 
ACACCCCGAC AAGGGACACC GGGGGCCCCG GGAGCAGAGA GACCTCAGAG CAGCCTCCTC 
CTGCCTCCTG TGGACGGCCG GCCCCAGCTG GTGATCCCAG CCAGTCCCAG CTTTCAGTTG 
CTGCCCCCAC CGACAGTCCT CAGTCCCTCC ATGATGGCTC CCCCGACAGC CGGCCCCCTT 
CCTGGCCCAG CTCTTCCGCC TGAGGACCCA GGGCCGGATC CGGAGAGCAG GTGGCTTTTC 
TTGAGCGCCA ACATTCTGCC CGTGGTGGAG CGGTGCATGG GTGCCATGCA AGAGGGGATG 
CAGATGGTGA AGCTGCGTGG CGGCTCCAAG GGCCTGGTCC GCTTCTACTA CCTGGACGAG 
CACCGCTCCT GCATCCGCTG GAGGCCCTCA CGCAAGAACG AGAAGGCCAA GATCTCCATC 
GACTCCATCC AGGAGGTGAG TGAGGGGCGG CAGTCGGAGG TCTTCCAGCG CTACCCTGAC 
GGCAGCTTCG ACCCCAACTG CTGCTTCAGC ATCTACCACG GCAGCCACCG CGAGTCGCTG 
GACCTGGTCT CCACCAGCAG CGAGGTGGCG CGCACCTGGG TCACTGGCCT GCGCTACCTC 
ATGGCCGGCA TCAGCGACGA GGACAGCCTG GCTCGCCGCC AGCGCACCAG GGACCAGTGG 
CTGAAGCAGA CGTTTGACGA GGCCGACAAG AACGGGGATG GCAGCCTGAG CATTGGCGAG 
GTCCTGCAGC TGCTGCACAA GCTCAACGTG AACCTGCCCC GGCAGAGGGT GAAGCAGATG 
TTCAGGGAAG CGGACACGGA TGACCACCAA GGGACGCTGG GTTTTGAAGA GTTCTGTGCC 
TTCTACAAGA TGATGTCCAC CCGCCGGGAC CTCTACCTGC TCATGCTGAC CTACAGCAAC 
CACAAGGACC ACCTGGATGC CGCCAGCCTG CAGCGCTTCC TGCAGGTGGA GCAGAAGATG 
GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC ATCATCGAGC AGTTTGAGCC ATGCCCAGAA 
AACAAGAGTA AGGGGCTGCT GGGCATTGAT GGCTTCACCA ACTACACCAG GAGCCCTGCT 
GGTGACATCT TCAACCCTGA GCACCACCAT GTGCACCAGG ACATGACGCA GCCGCTGAGC 
CACTACTTCA TCACCTCGTC CCACAACACC TACCTCGTGG GTGACCAGCT CATGTCCCAG 
TCACGGGTGG ACATGTATGC TTGGGTCCTG CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC 
TGCTGGGATG GGCCCGACGG GGAGCCCATT GTGCACCATG GCTACACTCT GACTTCCAAG 
ATCCTCTTCA AAGACGTCAT TGAAACCATC AACAAATATG CCTTCATCAA GAATGAGTAC 
CCAGTGATCC TGTCCATCGA AAACCACTGC AGTGTCATCC AGCAGAAGAA AATGGCCCAG 
TATCTGACTG ACATCCTTGG GGACAAGCTG GACCTGTCAT CAGTGAGCAG TGAAGATGCC 
ACCACACTCC CCTCTCCACA GATGCTCAAG GGCAAGATCC TCGTGAAGGG GAAGAAGCTC 
CCAGCCAACA TCAGCGAGGA TGCGGAGGAA GGCGAGGTGT CTGATGAGGA CAGTGCTGAT 
GAGATTGACG ATGACTGCAA GCTCCTCAAT GGGGATGCAT CCACCAATCG AAAGCGTGTA 
GAAAACACTG CTAAGAGGAA ACTGGATTCC CTCATCAAAG AGTCGAAGAT TCGGGACTGT 
GAGGACCCCA ACAACTTCTC CGTCTCCACA CTGTCCCCAT CTGGAAAGCT CGGACGCAAG 
AGCAAGGCTG AAGAGGACGT GGAGTCTGGG GAGGATGCCG GGGCCAGCAG ACGCAATGGC 
CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG 
GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG GACTCCCCGG GAGGCCAGAG CCGAGGGGCG 
ACCCGGCAGA AGAAGACCAT GAAGCTGTCC CGGGCCCTCT CTGACCTGGT GAAGTACACC 
AAGTCCGTGG CCACCCACGA CATAGAGATG GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC 
TTCAGCGAGA CCAAGGCCCA CCAGATTCTG CAGCAGAAGC CGGCGCAGTA CCTACGCTTC 
AACCAGCAGC AGCTCTCCCG CATCTACCCC TCCTCCTACC GTGTGGACTC CAGCAACTAC 
AACCCGCAGC CCTTCTGGAA CGCCGGCTGC CAAATGGTTG CCCTGAACTA CCAGTCAGAG 
GGGCGGATGC TGCAGCTGAA CCGAGCCAAG TTCAGCGCCA ACGGTGGCTG CGGCTACGTA 
CTCAAGCCTG GGTGCATGTG CCAGGGCGTG TTCAACCCCA ACTCGGAGGA CCCCCTGCCC 
GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG ATCATCAGTG GCCAGCAGCT TCCCAAGCCG 
CGCGACTCCA TGCTGGGGGA CCGTGGGGAG ATCATCGACC CCTTTGTGGA GGTGGAGATC 
ATTGGGCTCC CTGTGGACTG CAGCAGGGAG CAGACCCGCG TGGTGGACGA CAACGGTGAG 
GCTGGGCCGT GGCTCCGTCA CACCTGTGAT GGAAGTCTGA GGGGGGAGGG TTGGGGCTAC 
CTGGTGTGCC CGGGTGCCCT GCCCAGGCCT CCCTCAGTGA CAGTCCTGGG CGCCCTCCCC 
TCTAGGTGGG CAGTCCCGGA AGCAGCACCG GGAGTGGCAC TGGGAGTGGT GTGGGCCGGG 
GGCTCCAGGC TGGAGCGGTG CTGGGCCGGG CCCTCCCCAT GGGACACTCC TCCTGGCGCT 
GCTTCCCCAG GGTTCAACCC CACCTGGGAG GAGACCCTGG TTTTCATGGT GCACATGCCG 
GAGATCGCGC TGGTCCGCTT CCTCGTCTGG GACCACGATC CCATCGGGCG TGACTTCATT 
GGCCAGAGGA CGCTGGCCTT CAGCAGCATG ATGCCAGGCT ACAGACACGT GTACCTAGAA 
GGGATGGAAG AGGCCTCCAT CTTCGTGCAT GTGGCTGTCA GTGACATCAG CGGTAAGGTC 
AAGCAGGCTC TGGGCCTAAA AGGCCTCTTC CTCCGAGGCC CAAAGCCCGG CTCGCTGGAC 
AGTCATGCTG CTGGGCGGCC CCCGGCCCGG CCCTCCGTTA GCCAGCGGAT CCTGCGGCGC 
ACGGCCAGCG CCCCGACCAA GAGCCAGAAG CCGGGCCGCA GGGGCTTCCC GGAGCTGGTC 
CTGGGTACAC GGGACACAGG CTCCAAGGGG GTGGCAGACG ATGTGGTGCC CCCCGGGCCC 
GGACCTGCTC CGGAAGCCCC AGCCCAGGAG GGGCCCGGCA GCGGCAGCCC CCGAGGTAAG 
GCGCCAGCTG CGGTGGCAGA GAAGAGCCCT GTGCGAGTGC GGCCCCCGCG TGTCCTGGAC 
GGCCCCGGGC CTGCTGGGAT GGCCGCCACA TGCATGAAGT GTGTGGTGGG ATCCTGCGCC 
GGCGTGAACA CCGGGGGCCT GCAGAGGGAG CGGCCACCCA GCCCGGGGCC TGCAAGCAGG 
CAGGCAGCCA TTCGCCAGCA GCCCCGGGCC CGGGCTGACT CACTGGGGGC CCCCTGCTGT 
GGCCTGGACC CTCACGCTAT CCCGGGGAGA AGCAGAGAGG CCCCCAAGGG TCCTGGGGCC 
TGGAGGCAGG GTCCAGGCGG TAGCGGCTCC ATGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCGGGCATCC CCGAAAGGTC CCCCCGCTGG CCTGAGGGTG CCTGCAGGCA ACCGGGGGCC 
CTGCAGGGAG AGATGAGTGC CTTGTTTGCT CAAAAGCTGG AGGAGATCAG GAGTAAATCC 
CCCATGTTCT CCGCCGACAC CCGCCCCCTC TCCACGCAGC GGCCACTCCC CCCACTGTGC 
AGCCTGGAAA CCATCGCTGA GGAGCCCGCC CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT 
GTCCCCACCA GCTCTTCTCA GGGACGGCCC CCATACCCCA CAGGACCCGG AGCCAATGTG 
GCAAGCCCCC TAGAGGACAC TGAGGAGCCC CGAGACAGCA GGCCTCGGCC GTGCAACGGC 
GAGGGCGCCG GCGGGGCATA CGAGAGGGCC CCCGGCAGCC AGACGGACGG CAGGAGCCAG 
CCCCGGACCC TGGGCCACCT GCCCGTGATT AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC 
ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC 
TCCGATGCCA CGGGCAGTGA CCCGCTGTGG CAGCGGCTGG AGCCATGTGG CCACCGAGAC 
AGCGTTTCCT CCTCCTCCAG CATGTCATCC AGCGACACTG TCATTGACCT CTCCCTGCCC 
AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC CTCGCTGGAG CCCACATGGG ACGCCTGCCC 
CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC CCAGACCTGC CACCTGTGAC CAAGAGCAAA 
TCCAACCCCA ACCTTCGGGC TACAGGCCAG CGGCCTCCCA TACCTGACGA ACTGCAGCCC 
AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC 
CTGGTGGGCG TGCAGGACTG CCCCGTGGCT GCCAAGTCCA AGAGCCTGGG CGACCTCACT 
GCTGATGACT TTGCCCCTAG CTTTGAGGGC GGCTCCCGCA GACTGAGCCA CAGCCTGGGC 
CTCCCGGGAG GGACACGGCG GGTGTCGGGG CCAGGGGTGA GACGGGACAC CCTGACAGAG 
CAGCTGCGCT GGCTCACTGT CTTCCAGCAG GCAGGAGACA TCACGTCACC CACCAGCCTG 
GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC 
AGCCACAGCC GCGTGCGTGC CATTGCCAGC CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG 
AGACTGCAGG GCCTGGGCCG GCAGGGACCC CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC 
GCCTGCTCCG TGGGCCACGA GGGCAGTGTG GATGCACCAG CACCCTCCAA GGGAGCCCTC 
GGGCCAGCAT CCGCGGCTGC TGAAAACCTG GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306826.1
CDS	16..5283
Translation	MSEEQAGACGGLRYLCWELAIQGTQREGPPQVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGEAGPWLRHTCDGSLRGEGWGYLVCPGALPRPPSVTVLGALPSRWAVPEAAPGVALGVVWAGGSRLERCWAGPSPWDTPPGAASPGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_024451058.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451058.1

ORF Insert Sequence:

ATGTCAGAGG AGCAGGCCGG GGCTTGTGGG GGCCTCCGGT ACCTCTGCTG GGAGCTCGCG 
ATCCAAGGGA CACAGCGTGA GGGGCCACCG CAGGTGTGTG ATGCGCTGCT GGCCCAGGCT 
ACACCCCGAC AAGGGACACC GGGGGCCCCG GGAGCAGAGA GACCTCAGAG CAGCCTCCTC 
CTGCCTCCTG TGGACGGCCG GCCCCAGCTG GTGATCCCAG CCAGTCCCAG CTTTCAGTTG 
CTGCCCCCAC CGACAGTCCT CAGTCCCTCC ATGATGGCTC CCCCGACAGC CGGCCCCCTT 
CCTGGCCCAG CTCTTCCGCC TGAGGACCCA GGGCCGGATC CGGAGAGCAG GTGGCTTTTC 
TTGAGCGCCA ACATTCTGCC CGTGGTGGAG CGGTGCATGG GTGCCATGCA AGAGGGGATG 
CAGATGGTGA AGCTGCGTGG CGGCTCCAAG GGCCTGGTCC GCTTCTACTA CCTGGACGAG 
CACCGCTCCT GCATCCGCTG GAGGCCCTCA CGCAAGAACG AGAAGGCCAA GATCTCCATC 
GACTCCATCC AGGAGGTGAG TGAGGGGCGG CAGTCGGAGG TCTTCCAGCG CTACCCTGAC 
GGCAGCTTCG ACCCCAACTG CTGCTTCAGC ATCTACCACG GCAGCCACCG CGAGTCGCTG 
GACCTGGTCT CCACCAGCAG CGAGGTGGCG CGCACCTGGG TCACTGGCCT GCGCTACCTC 
ATGGCCGGCA TCAGCGACGA GGACAGCCTG GCTCGCCGCC AGCGCACCAG GGACCAGTGG 
CTGAAGCAGA CGTTTGACGA GGCCGACAAG AACGGGGATG GCAGCCTGAG CATTGGCGAG 
GTCCTGCAGC TGCTGCACAA GCTCAACGTG AACCTGCCCC GGCAGAGGGT GAAGCAGATG 
TTCAGGGAAG CGGACACGGA TGACCACCAA GGGACGCTGG GTTTTGAAGA GTTCTGTGCC 
TTCTACAAGA TGATGTCCAC CCGCCGGGAC CTCTACCTGC TCATGCTGAC CTACAGCAAC 
CACAAGGACC ACCTGGATGC CGCCAGCCTG CAGCGCTTCC TGCAGGTGGA GCAGAAGATG 
GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC ATCATCGAGC AGTTTGAGCC ATGCCCAGAA 
AACAAGAGTA AGGGGCTGCT GGGCATTGAT GGCTTCACCA ACTACACCAG GAGCCCTGCT 
GGTGACATCT TCAACCCTGA GCACCACCAT GTGCACCAGG ACATGACGCA GCCGCTGAGC 
CACTACTTCA TCACCTCGTC CCACAACACC TACCTCGTGG GTGACCAGCT CATGTCCCAG 
TCACGGGTGG ACATGTATGC TTGGGTCCTG CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC 
TGCTGGGATG GGCCCGACGG GGAGCCCATT GTGCACCATG GCTACACTCT GACTTCCAAG 
ATCCTCTTCA AAGACGTCAT TGAAACCATC AACAAATATG CCTTCATCAA GAATGAGTAC 
CCAGTGATCC TGTCCATCGA AAACCACTGC AGTGTCATCC AGCAGAAGAA AATGGCCCAG 
TATCTGACTG ACATCCTTGG GGACAAGCTG GACCTGTCAT CAGTGAGCAG TGAAGATGCC 
ACCACACTCC CCTCTCCACA GATGCTCAAG GGCAAGATCC TCGTGAAGGG GAAGAAGCTC 
CCAGCCAACA TCAGCGAGGA TGCGGAGGAA GGCGAGGTGT CTGATGAGGA CAGTGCTGAT 
GAGATTGACG ATGACTGCAA GCTCCTCAAT GGGGATGCAT CCACCAATCG AAAGCGTGTA 
GAAAACACTG CTAAGAGGAA ACTGGATTCC CTCATCAAAG AGTCGAAGAT TCGGGACTGT 
GAGGACCCCA ACAACTTCTC CGTCTCCACA CTGTCCCCAT CTGGAAAGCT CGGACGCAAG 
AGCAAGGCTG AAGAGGACGT GGAGTCTGGG GAGGATGCCG GGGCCAGCAG ACGCAATGGC 
CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG 
GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG GACTCCCCGG GAGGCCAGAG CCGAGGGGCG 
ACCCGGCAGA AGAAGACCAT GAAGCTGTCC CGGGCCCTCT CTGACCTGGT GAAGTACACC 
AAGTCCGTGG CCACCCACGA CATAGAGATG GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC 
TTCAGCGAGA CCAAGGCCCA CCAGATTCTG CAGCAGAAGC CGGCGCAGTA CCTACGCTTC 
AACCAGCAGC AGCTCTCCCG CATCTACCCC TCCTCCTACC GTGTGGACTC CAGCAACTAC 
AACCCGCAGC CCTTCTGGAA CGCCGGCTGC CAAATGGTTG CCCTGAACTA CCAGTCAGAG 
GGGCGGATGC TGCAGCTGAA CCGAGCCAAG TTCAGCGCCA ACGGTGGCTG CGGCTACGTA 
CTCAAGCCTG GGTGCATGTG CCAGGGCGTG TTCAACCCCA ACTCGGAGGA CCCCCTGCCC 
GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG ATCATCAGTG GCCAGCAGCT TCCCAAGCCG 
CGCGACTCCA TGCTGGGGGA CCGTGGGGAG ATCATCGACC CCTTTGTGGA GGTGGAGATC 
ATTGGGCTCC CTGTGGACTG CAGCAGGGAG CAGACCCGCG TGGTGGACGA CAACGGTGAG 
GCTGGGCCGT GGCTCCGTCA CACCTGTGAT GGAAGTCTGA GGGGGGAGGG TTGGGGCTAC 
CTGGTGTGCC CGGGTGCCCT GCCCAGGCCT CCCTCAGTGA CAGTCCTGGG CGCCCTCCCC 
TCTAGGTGGG CAGTCCCGGA AGCAGCACCG GGAGTGGCAC TGGGAGTGGT GTGGGCCGGG 
GGCTCCAGGC TGGAGCGGTG CTGGGCCGGG CCCTCCCCAT GGGACACTCC TCCTGGCGCT 
GCTTCCCCAG GGTTCAACCC CACCTGGGAG GAGACCCTGG TTTTCATGGT GCACATGCCG 
GAGATCGCGC TGGTCCGCTT CCTCGTCTGG GACCACGATC CCATCGGGCG TGACTTCATT 
GGCCAGAGGA CGCTGGCCTT CAGCAGCATG ATGCCAGGCT ACAGACACGT GTACCTAGAA 
GGGATGGAAG AGGCCTCCAT CTTCGTGCAT GTGGCTGTCA GTGACATCAG CGGTAAGGTC 
AAGCAGGCTC TGGGCCTAAA AGGCCTCTTC CTCCGAGGCC CAAAGCCCGG CTCGCTGGAC 
AGTCATGCTG CTGGGCGGCC CCCGGCCCGG CCCTCCGTTA GCCAGCGGAT CCTGCGGCGC 
ACGGCCAGCG CCCCGACCAA GAGCCAGAAG CCGGGCCGCA GGGGCTTCCC GGAGCTGGTC 
CTGGGTACAC GGGACACAGG CTCCAAGGGG GTGGCAGACG ATGTGGTGCC CCCCGGGCCC 
GGACCTGCTC CGGAAGCCCC AGCCCAGGAG GGGCCCGGCA GCGGCAGCCC CCGAGGTAAG 
GCGCCAGCTG CGGTGGCAGA GAAGAGCCCT GTGCGAGTGC GGCCCCCGCG TGTCCTGGAC 
GGCCCCGGGC CTGCTGGGAT GGCCGCCACA TGCATGAAGT GTGTGGTGGG ATCCTGCGCC 
GGCGTGAACA CCGGGGGCCT GCAGAGGGAG CGGCCACCCA GCCCGGGGCC TGCAAGCAGG 
CAGGCAGCCA TTCGCCAGCA GCCCCGGGCC CGGGCTGACT CACTGGGGGC CCCCTGCTGT 
GGCCTGGACC CTCACGCTAT CCCGGGGAGA AGCAGAGAGG CCCCCAAGGG TCCTGGGGCC 
TGGAGGCAGG GTCCAGGCGG TAGCGGCTCC ATGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCGGGCATCC CCGAAAGGTC CCCCCGCTGG CCTGAGGGTG CCTGCAGGCA ACCGGGGGCC 
CTGCAGGGAG AGATGAGTGC CTTGTTTGCT CAAAAGCTGG AGGAGATCAG GAGTAAATCC 
CCCATGTTCT CCGCCGACAC CCGCCCCCTC TCCACGCAGC GGCCACTCCC CCCACTGTGC 
AGCCTGGAAA CCATCGCTGA GGAGCCCGCC CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT 
GTCCCCACCA GCTCTTCTCA GGGACGGCCC CCATACCCCA CAGGACCCGG AGCCAATGTG 
GCAAGCCCCC TAGAGGACAC TGAGGAGCCC CGAGACAGCA GGCCTCGGCC GTGCAACGGC 
GAGGGCGCCG GCGGGGCATA CGAGAGGGCC CCCGGCAGCC AGACGGACGG CAGGAGCCAG 
CCCCGGACCC TGGGCCACCT GCCCGTGATT AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC 
ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC 
TCCGATGCCA CGGGCAGTGA CCCGCTGTGG CAGCGGCTGG AGCCATGTGG CCACCGAGAC 
AGCGTTTCCT CCTCCTCCAG CATGTCATCC AGCGACACTG TCATTGACCT CTCCCTGCCC 
AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC CTCGCTGGAG CCCACATGGG ACGCCTGCCC 
CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC CCAGACCTGC CACCTGTGAC CAAGAGCAAA 
TCCAACCCCA ACCTTCGGGC TACAGGCCAG CGGCCTCCCA TACCTGACGA ACTGCAGCCC 
AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC 
CTGGTGGGCG TGCAGGACTG CCCCGTGGCT GCCAAGTCCA AGAGCCTGGG CGACCTCACT 
GCTGATGACT TTGCCCCTAG CTTTGAGGGC GGCTCCCGCA GACTGAGCCA CAGCCTGGGC 
CTCCCGGGAG GGACACGGCG GGTGTCGGGG CCAGGGGTGA GACGGGACAC CCTGACAGAG 
CAGCTGCGCT GGCTCACTGT CTTCCAGCAG GCAGGAGACA TCACGTCACC CACCAGCCTG 
GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC 
AGCCACAGCC GCGTGCGTGC CATTGCCAGC CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG 
AGACTGCAGG GCCTGGGCCG GCAGGGACCC CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC 
GCCTGCTCCG TGGGCCACGA GGGCAGTGTG GATGCACCAG CACCCTCCAA GGGAGCCCTC 
GGGCCAGCAT CCGCGGCTGC TGAAAACCTG GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_024451059.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306827.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

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CloneID	OHu106376
Clone ID Related Accession (Same CDS sequence)	XM_024451059.1
Accession Version	XM_024451059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG TGAGGCTGGG CCGTGGCTCC GTCACACCTG TGATGGAAGT 
CTGAGGGGGG AGGGTTGGGG CTACCTGGTG TGCCCGGGTG CCCTGCCCAG GCCTCCCTCA 
GTGACAGTCC TGGGCGCCCT CCCCTCTAGG TGGGCAGTCC CGGAAGCAGC ACCGGGAGTG 
GCACTGGGAG TGGTGTGGGC CGGGGGCTCC AGGCTGGAGC GGTGCTGGGC CGGGCCCTCC 
CCATGGGACA CTCCTCCTGG CGCTGCTTCC CCAGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGA CACCCGCCCC CTCTCCACGC AGCGGCCACT CCCCCCACTG 
TGCAGCCTGG AAACCATCGC TGAGGAGCCC GCCCCAGGCC CTGGTCCCCC GCCACCAGCG 
GCTGTCCCCA CCAGCTCTTC TCAGGGACGG CCCCCATACC CCACAGGACC CGGAGCCAAT 
GTGGCAAGCC CCCTAGAGGA CACTGAGGAG CCCCGAGACA GCAGGCCTCG GCCGTGCAAC 
GGCGAGGGCG CCGGCGGGGC ATACGAGAGG GCCCCCGGCA GCCAGACGGA CGGCAGGAGC 
CAGCCCCGGA CCCTGGGCCA CCTGCCCGTG ATTAGAAGGG TGAAGAGTGA GGGGCAGGTG 
CCCACGGAGC CCCTGGGAGG GTGGCGGCCC CTGGCCGCTC CCTTTCCAGC TCCTGCCGTG 
TACTCCGATG CCACGGGCAG TGACCCGCTG TGGCAGCGGC TGGAGCCATG TGGCCACCGA 
GACAGCGTTT CCTCCTCCTC CAGCATGTCA TCCAGCGACA CTGTCATTGA CCTCTCCCTG 
CCCAGCCTGG GCCTGGGCCG CAGCCGTGAG AACCTCGCTG GAGCCCACAT GGGACGCCTG 
CCCCCCAGGC CCCACTCGGC TTCGGCTGCC CGCCCAGACC TGCCACCTGT GACCAAGAGC 
AAATCCAACC CCAACCTTCG GGCTACAGGC CAGCGGCCTC CCATACCTGA CGAACTGCAG 
CCCAGGTCCC TGGCCCCAAG GATGGCTGGC CTCCCCTTCC GGCCTCCCTG GGGCTGCCTT 
TCCCTGGTGG GCGTGCAGGA CTGCCCCGTG GCTGCCAAGT CCAAGAGCCT GGGCGACCTC 
ACTGCTGATG ACTTTGCCCC TAGCTTTGAG GGCGGCTCCC GCAGACTGAG CCACAGCCTG 
GGCCTCCCGG GAGGGACACG GCGGGTGTCG GGGCCAGGGG TGAGACGGGA CACCCTGACA 
GAGCAGCTGC GCTGGCTCAC TGTCTTCCAG CAGGCAGGAG ACATCACGTC ACCCACCAGC 
CTGGGCCCGG CTGGGGAGGG GGTGGCAGGG GGCCCTGGTT TTGTGCGGCG CTCCTCCTCC 
CGCAGCCACA GCCGCGTGCG TGCCATTGCC AGCCGGGCCC GCCAGGCCCA GGAGCGGCAG 
CAGAGACTGC AGGGCCTGGG CCGGCAGGGA CCCCCAGAAG AGGAGCGGGG CACCCCCGAG 
GGCGCCTGCT CCGTGGGCCA CGAGGGCAGT GTGGATGCAC CAGCACCCTC CAAGGGAGCC 
CTCGGGCCAG CATCCGCGGC TGCTGAAAAC CTGGTCCTGC TCCGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306827.1
CDS	21..4811
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGEAGPWLRHTCDGSLRGEGWGYLVCPGALPRPPSVTVLGALPSRWAVPEAAPGVALGVVWAGGSRLERCWAGPSPWDTPPGAASPGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRDTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_024451059.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451059.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG TGAGGCTGGG CCGTGGCTCC GTCACACCTG TGATGGAAGT 
CTGAGGGGGG AGGGTTGGGG CTACCTGGTG TGCCCGGGTG CCCTGCCCAG GCCTCCCTCA 
GTGACAGTCC TGGGCGCCCT CCCCTCTAGG TGGGCAGTCC CGGAAGCAGC ACCGGGAGTG 
GCACTGGGAG TGGTGTGGGC CGGGGGCTCC AGGCTGGAGC GGTGCTGGGC CGGGCCCTCC 
CCATGGGACA CTCCTCCTGG CGCTGCTTCC CCAGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGA CACCCGCCCC CTCTCCACGC AGCGGCCACT CCCCCCACTG 
TGCAGCCTGG AAACCATCGC TGAGGAGCCC GCCCCAGGCC CTGGTCCCCC GCCACCAGCG 
GCTGTCCCCA CCAGCTCTTC TCAGGGACGG CCCCCATACC CCACAGGACC CGGAGCCAAT 
GTGGCAAGCC CCCTAGAGGA CACTGAGGAG CCCCGAGACA GCAGGCCTCG GCCGTGCAAC 
GGCGAGGGCG CCGGCGGGGC ATACGAGAGG GCCCCCGGCA GCCAGACGGA CGGCAGGAGC 
CAGCCCCGGA CCCTGGGCCA CCTGCCCGTG ATTAGAAGGG TGAAGAGTGA GGGGCAGGTG 
CCCACGGAGC CCCTGGGAGG GTGGCGGCCC CTGGCCGCTC CCTTTCCAGC TCCTGCCGTG 
TACTCCGATG CCACGGGCAG TGACCCGCTG TGGCAGCGGC TGGAGCCATG TGGCCACCGA 
GACAGCGTTT CCTCCTCCTC CAGCATGTCA TCCAGCGACA CTGTCATTGA CCTCTCCCTG 
CCCAGCCTGG GCCTGGGCCG CAGCCGTGAG AACCTCGCTG GAGCCCACAT GGGACGCCTG 
CCCCCCAGGC CCCACTCGGC TTCGGCTGCC CGCCCAGACC TGCCACCTGT GACCAAGAGC 
AAATCCAACC CCAACCTTCG GGCTACAGGC CAGCGGCCTC CCATACCTGA CGAACTGCAG 
CCCAGGTCCC TGGCCCCAAG GATGGCTGGC CTCCCCTTCC GGCCTCCCTG GGGCTGCCTT 
TCCCTGGTGG GCGTGCAGGA CTGCCCCGTG GCTGCCAAGT CCAAGAGCCT GGGCGACCTC 
ACTGCTGATG ACTTTGCCCC TAGCTTTGAG GGCGGCTCCC GCAGACTGAG CCACAGCCTG 
GGCCTCCCGG GAGGGACACG GCGGGTGTCG GGGCCAGGGG TGAGACGGGA CACCCTGACA 
GAGCAGCTGC GCTGGCTCAC TGTCTTCCAG CAGGCAGGAG ACATCACGTC ACCCACCAGC 
CTGGGCCCGG CTGGGGAGGG GGTGGCAGGG GGCCCTGGTT TTGTGCGGCG CTCCTCCTCC 
CGCAGCCACA GCCGCGTGCG TGCCATTGCC AGCCGGGCCC GCCAGGCCCA GGAGCGGCAG 
CAGAGACTGC AGGGCCTGGG CCGGCAGGGA CCCCCAGAAG AGGAGCGGGG CACCCCCGAG 
GGCGCCTGCT CCGTGGGCCA CGAGGGCAGT GTGGATGCAC CAGCACCCTC CAAGGGAGCC 
CTCGGGCCAG CATCCGCGGC TGCTGAAAAC CTGGTCCTGC TCCGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_024451060.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306828.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

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CloneID	OHu106377
Clone ID Related Accession (Same CDS sequence)	XM_024451060.1 , XM_024451061.1
Accession Version	XM_024451060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGTGAG GCTGGGCCGT GGCTCCGTCA CACCTGTGAT 
GGAAGTCTGA GGGGGGAGGG TTGGGGCTAC CTGGTGTGCC CGGGTGCCCT GCCCAGGCCT 
CCCTCAGTGA CAGTCCTGGG CGCCCTCCCC TCTAGGTGGG CAGTCCCGGA AGCAGCACCG 
GGAGTGGCAC TGGGAGTGGT GTGGGCCGGG GGCTCCAGGC TGGAGCGGTG CTGGGCCGGG 
CCCTCCCCAT GGGACACTCC TCCTGGCGCT GCTTCCCCAG GGTTCAACCC CACCTGGGAG 
GAGACCCTGG TTTTCATGGT GCACATGCCG GAGATCGCGC TGGTCCGCTT CCTCGTCTGG 
GACCACGATC CCATCGGGCG TGACTTCATT GGCCAGAGGA CGCTGGCCTT CAGCAGCATG 
ATGCCAGGCT ACAGACACGT GTACCTAGAA GGGATGGAAG AGGCCTCCAT CTTCGTGCAT 
GTGGCTGTCA GTGACATCAG CGGTAAGGTC AAGCAGGCTC TGGGCCTAAA AGGCCTCTTC 
CTCCGAGGCC CAAAGCCCGG CTCGCTGGAC AGTCATGCTG CTGGGCGGCC CCCGGCCCGG 
CCCTCCGTTA GCCAGCGGAT CCTGCGGCGC ACGGCCAGCG CCCCGACCAA GAGCCAGAAG 
CCGGGCCGCA GGGGCTTCCC GGAGCTGGTC CTGGGTACAC GGGACACAGG CTCCAAGGGG 
GTGGCAGACG ATGTGGTGCC CCCCGGGCCC GGACCTGCTC CGGAAGCCCC AGCCCAGGAG 
GGGCCCGGCA GCGGCAGCCC CCGAGGTAAG GCGCCAGCTG CGGTGGCAGA GAAGAGCCCT 
GTGCGAGTGC GGCCCCCGCG TGTCCTGGAC GGCCCCGGGC CTGCTGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG ATCCTGCGCC GGCGTGAACA CCGGGGGCCT GCAGAGGGAG 
CGGCCACCCA GCCCGGGGCC TGCAAGCAGG CAGGCAGCCA TTCGCCAGCA GCCCCGGGCC 
CGGGCTGACT CACTGGGGGC CCCCTGCTGT GGCCTGGACC CTCACGCTAT CCCGGGGAGA 
AGCAGAGAGG CCCCCAAGGG TCCTGGGGCC TGGAGGCAGG GTCCAGGCGG TAGCGGCTCC 
ATGTCCTCGG ACTCCAGCAG CCCAGACAGC CCGGGCATCC CCGAAAGGTC CCCCCGCTGG 
CCTGAGGGTG CCTGCAGGCA ACCGGGGGCC CTGCAGGGAG AGATGAGTGC CTTGTTTGCT 
CAAAAGCTGG AGGAGATCAG GAGTAAATCC CCCATGTTCT CCGCCGACAC CCGCCCCCTC 
TCCACGCAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA GGAGCCCGCC 
CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT GTCCCCACCA GCTCTTCTCA GGGACGGCCC 
CCATACCCCA CAGGACCCGG AGCCAATGTG GCAAGCCCCC TAGAGGACAC TGAGGAGCCC 
CGAGACAGCA GGCCTCGGCC GTGCAACGGC GAGGGCGCCG GCGGGGCATA CGAGAGGGCC 
CCCGGCAGCC AGACGGACGG CAGGAGCCAG CCCCGGACCC TGGGCCACCT GCCCGTGATT 
AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG 
GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC TCCGATGCCA CGGGCAGTGA CCCGCTGTGG 
CAGCGGCTGG AGCCATGTGG CCACCGAGAC AGCGTTTCCT CCTCCTCCAG CATGTCATCC 
AGCGACACTG TCATTGACCT CTCCCTGCCC AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC 
CTCGCTGGAG CCCACATGGG ACGCCTGCCC CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC 
CCAGACCTGC CACCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTTCGGGC TACAGGCCAG 
CGGCCTCCCA TACCTGACGA ACTGCAGCCC AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC 
CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC CTGGTGGGCG TGCAGGACTG CCCCGTGGCT 
GCCAAGTCCA AGAGCCTGGG CGACCTCACT GCTGATGACT TTGCCCCTAG CTTTGAGGGC 
GGCTCCCGCA GACTGAGCCA CAGCCTGGGC CTCCCGGGAG GGACACGGCG GGTGTCGGGG 
CCAGGGGTGA GACGGGACAC CCTGACAGAG CAGCTGCGCT GGCTCACTGT CTTCCAGCAG 
GCAGGAGACA TCACGTCACC CACCAGCCTG GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC 
CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC AGCCACAGCC GCGTGCGTGC CATTGCCAGC 
CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG AGACTGCAGG GCCTGGGCCG GCAGGGACCC 
CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC GCCTGCTCCG TGGGCCACGA GGGCAGTGTG 
GATGCACCAG CACCCTCCAA GGGAGCCCTC GGGCCAGCAT CCGCGGCTGC TGAAAACCTG 
GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306828.1
CDS	339..5336
Translation	MMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGEAGPWLRHTCDGSLRGEGWGYLVCPGALPRPPSVTVLGALPSRWAVPEAAPGVALGVVWAGGSRLERCWAGPSPWDTPPGAASPGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_024451060.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451060.1

ORF Insert Sequence:

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGTGAG GCTGGGCCGT GGCTCCGTCA CACCTGTGAT 
GGAAGTCTGA GGGGGGAGGG TTGGGGCTAC CTGGTGTGCC CGGGTGCCCT GCCCAGGCCT 
CCCTCAGTGA CAGTCCTGGG CGCCCTCCCC TCTAGGTGGG CAGTCCCGGA AGCAGCACCG 
GGAGTGGCAC TGGGAGTGGT GTGGGCCGGG GGCTCCAGGC TGGAGCGGTG CTGGGCCGGG 
CCCTCCCCAT GGGACACTCC TCCTGGCGCT GCTTCCCCAG GGTTCAACCC CACCTGGGAG 
GAGACCCTGG TTTTCATGGT GCACATGCCG GAGATCGCGC TGGTCCGCTT CCTCGTCTGG 
GACCACGATC CCATCGGGCG TGACTTCATT GGCCAGAGGA CGCTGGCCTT CAGCAGCATG 
ATGCCAGGCT ACAGACACGT GTACCTAGAA GGGATGGAAG AGGCCTCCAT CTTCGTGCAT 
GTGGCTGTCA GTGACATCAG CGGTAAGGTC AAGCAGGCTC TGGGCCTAAA AGGCCTCTTC 
CTCCGAGGCC CAAAGCCCGG CTCGCTGGAC AGTCATGCTG CTGGGCGGCC CCCGGCCCGG 
CCCTCCGTTA GCCAGCGGAT CCTGCGGCGC ACGGCCAGCG CCCCGACCAA GAGCCAGAAG 
CCGGGCCGCA GGGGCTTCCC GGAGCTGGTC CTGGGTACAC GGGACACAGG CTCCAAGGGG 
GTGGCAGACG ATGTGGTGCC CCCCGGGCCC GGACCTGCTC CGGAAGCCCC AGCCCAGGAG 
GGGCCCGGCA GCGGCAGCCC CCGAGGTAAG GCGCCAGCTG CGGTGGCAGA GAAGAGCCCT 
GTGCGAGTGC GGCCCCCGCG TGTCCTGGAC GGCCCCGGGC CTGCTGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG ATCCTGCGCC GGCGTGAACA CCGGGGGCCT GCAGAGGGAG 
CGGCCACCCA GCCCGGGGCC TGCAAGCAGG CAGGCAGCCA TTCGCCAGCA GCCCCGGGCC 
CGGGCTGACT CACTGGGGGC CCCCTGCTGT GGCCTGGACC CTCACGCTAT CCCGGGGAGA 
AGCAGAGAGG CCCCCAAGGG TCCTGGGGCC TGGAGGCAGG GTCCAGGCGG TAGCGGCTCC 
ATGTCCTCGG ACTCCAGCAG CCCAGACAGC CCGGGCATCC CCGAAAGGTC CCCCCGCTGG 
CCTGAGGGTG CCTGCAGGCA ACCGGGGGCC CTGCAGGGAG AGATGAGTGC CTTGTTTGCT 
CAAAAGCTGG AGGAGATCAG GAGTAAATCC CCCATGTTCT CCGCCGACAC CCGCCCCCTC 
TCCACGCAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA GGAGCCCGCC 
CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT GTCCCCACCA GCTCTTCTCA GGGACGGCCC 
CCATACCCCA CAGGACCCGG AGCCAATGTG GCAAGCCCCC TAGAGGACAC TGAGGAGCCC 
CGAGACAGCA GGCCTCGGCC GTGCAACGGC GAGGGCGCCG GCGGGGCATA CGAGAGGGCC 
CCCGGCAGCC AGACGGACGG CAGGAGCCAG CCCCGGACCC TGGGCCACCT GCCCGTGATT 
AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG 
GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC TCCGATGCCA CGGGCAGTGA CCCGCTGTGG 
CAGCGGCTGG AGCCATGTGG CCACCGAGAC AGCGTTTCCT CCTCCTCCAG CATGTCATCC 
AGCGACACTG TCATTGACCT CTCCCTGCCC AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC 
CTCGCTGGAG CCCACATGGG ACGCCTGCCC CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC 
CCAGACCTGC CACCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTTCGGGC TACAGGCCAG 
CGGCCTCCCA TACCTGACGA ACTGCAGCCC AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC 
CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC CTGGTGGGCG TGCAGGACTG CCCCGTGGCT 
GCCAAGTCCA AGAGCCTGGG CGACCTCACT GCTGATGACT TTGCCCCTAG CTTTGAGGGC 
GGCTCCCGCA GACTGAGCCA CAGCCTGGGC CTCCCGGGAG GGACACGGCG GGTGTCGGGG 
CCAGGGGTGA GACGGGACAC CCTGACAGAG CAGCTGCGCT GGCTCACTGT CTTCCAGCAG 
GCAGGAGACA TCACGTCACC CACCAGCCTG GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC 
CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC AGCCACAGCC GCGTGCGTGC CATTGCCAGC 
CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG AGACTGCAGG GCCTGGGCCG GCAGGGACCC 
CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC GCCTGCTCCG TGGGCCACGA GGGCAGTGTG 
GATGCACCAG CACCCTCCAA GGGAGCCCTC GGGCCAGCAT CCGCGGCTGC TGAAAACCTG 
GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_024451061.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306829.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

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CloneID	OHu106377
Clone ID Related Accession (Same CDS sequence)	XM_024451060.1 , XM_024451061.1
Accession Version	XM_024451061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGTGAG GCTGGGCCGT GGCTCCGTCA CACCTGTGAT 
GGAAGTCTGA GGGGGGAGGG TTGGGGCTAC CTGGTGTGCC CGGGTGCCCT GCCCAGGCCT 
CCCTCAGTGA CAGTCCTGGG CGCCCTCCCC TCTAGGTGGG CAGTCCCGGA AGCAGCACCG 
GGAGTGGCAC TGGGAGTGGT GTGGGCCGGG GGCTCCAGGC TGGAGCGGTG CTGGGCCGGG 
CCCTCCCCAT GGGACACTCC TCCTGGCGCT GCTTCCCCAG GGTTCAACCC CACCTGGGAG 
GAGACCCTGG TTTTCATGGT GCACATGCCG GAGATCGCGC TGGTCCGCTT CCTCGTCTGG 
GACCACGATC CCATCGGGCG TGACTTCATT GGCCAGAGGA CGCTGGCCTT CAGCAGCATG 
ATGCCAGGCT ACAGACACGT GTACCTAGAA GGGATGGAAG AGGCCTCCAT CTTCGTGCAT 
GTGGCTGTCA GTGACATCAG CGGTAAGGTC AAGCAGGCTC TGGGCCTAAA AGGCCTCTTC 
CTCCGAGGCC CAAAGCCCGG CTCGCTGGAC AGTCATGCTG CTGGGCGGCC CCCGGCCCGG 
CCCTCCGTTA GCCAGCGGAT CCTGCGGCGC ACGGCCAGCG CCCCGACCAA GAGCCAGAAG 
CCGGGCCGCA GGGGCTTCCC GGAGCTGGTC CTGGGTACAC GGGACACAGG CTCCAAGGGG 
GTGGCAGACG ATGTGGTGCC CCCCGGGCCC GGACCTGCTC CGGAAGCCCC AGCCCAGGAG 
GGGCCCGGCA GCGGCAGCCC CCGAGGTAAG GCGCCAGCTG CGGTGGCAGA GAAGAGCCCT 
GTGCGAGTGC GGCCCCCGCG TGTCCTGGAC GGCCCCGGGC CTGCTGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG ATCCTGCGCC GGCGTGAACA CCGGGGGCCT GCAGAGGGAG 
CGGCCACCCA GCCCGGGGCC TGCAAGCAGG CAGGCAGCCA TTCGCCAGCA GCCCCGGGCC 
CGGGCTGACT CACTGGGGGC CCCCTGCTGT GGCCTGGACC CTCACGCTAT CCCGGGGAGA 
AGCAGAGAGG CCCCCAAGGG TCCTGGGGCC TGGAGGCAGG GTCCAGGCGG TAGCGGCTCC 
ATGTCCTCGG ACTCCAGCAG CCCAGACAGC CCGGGCATCC CCGAAAGGTC CCCCCGCTGG 
CCTGAGGGTG CCTGCAGGCA ACCGGGGGCC CTGCAGGGAG AGATGAGTGC CTTGTTTGCT 
CAAAAGCTGG AGGAGATCAG GAGTAAATCC CCCATGTTCT CCGCCGACAC CCGCCCCCTC 
TCCACGCAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA GGAGCCCGCC 
CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT GTCCCCACCA GCTCTTCTCA GGGACGGCCC 
CCATACCCCA CAGGACCCGG AGCCAATGTG GCAAGCCCCC TAGAGGACAC TGAGGAGCCC 
CGAGACAGCA GGCCTCGGCC GTGCAACGGC GAGGGCGCCG GCGGGGCATA CGAGAGGGCC 
CCCGGCAGCC AGACGGACGG CAGGAGCCAG CCCCGGACCC TGGGCCACCT GCCCGTGATT 
AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG 
GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC TCCGATGCCA CGGGCAGTGA CCCGCTGTGG 
CAGCGGCTGG AGCCATGTGG CCACCGAGAC AGCGTTTCCT CCTCCTCCAG CATGTCATCC 
AGCGACACTG TCATTGACCT CTCCCTGCCC AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC 
CTCGCTGGAG CCCACATGGG ACGCCTGCCC CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC 
CCAGACCTGC CACCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTTCGGGC TACAGGCCAG 
CGGCCTCCCA TACCTGACGA ACTGCAGCCC AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC 
CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC CTGGTGGGCG TGCAGGACTG CCCCGTGGCT 
GCCAAGTCCA AGAGCCTGGG CGACCTCACT GCTGATGACT TTGCCCCTAG CTTTGAGGGC 
GGCTCCCGCA GACTGAGCCA CAGCCTGGGC CTCCCGGGAG GGACACGGCG GGTGTCGGGG 
CCAGGGGTGA GACGGGACAC CCTGACAGAG CAGCTGCGCT GGCTCACTGT CTTCCAGCAG 
GCAGGAGACA TCACGTCACC CACCAGCCTG GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC 
CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC AGCCACAGCC GCGTGCGTGC CATTGCCAGC 
CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG AGACTGCAGG GCCTGGGCCG GCAGGGACCC 
CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC GCCTGCTCCG TGGGCCACGA GGGCAGTGTG 
GATGCACCAG CACCCTCCAA GGGAGCCCTC GGGCCAGCAT CCGCGGCTGC TGAAAACCTG 
GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306829.1
CDS	283..5280
Translation	MMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGEAGPWLRHTCDGSLRGEGWGYLVCPGALPRPPSVTVLGALPSRWAVPEAAPGVALGVVWAGGSRLERCWAGPSPWDTPPGAASPGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_024451061.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451061.1

ORF Insert Sequence:

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGTGAG GCTGGGCCGT GGCTCCGTCA CACCTGTGAT 
GGAAGTCTGA GGGGGGAGGG TTGGGGCTAC CTGGTGTGCC CGGGTGCCCT GCCCAGGCCT 
CCCTCAGTGA CAGTCCTGGG CGCCCTCCCC TCTAGGTGGG CAGTCCCGGA AGCAGCACCG 
GGAGTGGCAC TGGGAGTGGT GTGGGCCGGG GGCTCCAGGC TGGAGCGGTG CTGGGCCGGG 
CCCTCCCCAT GGGACACTCC TCCTGGCGCT GCTTCCCCAG GGTTCAACCC CACCTGGGAG 
GAGACCCTGG TTTTCATGGT GCACATGCCG GAGATCGCGC TGGTCCGCTT CCTCGTCTGG 
GACCACGATC CCATCGGGCG TGACTTCATT GGCCAGAGGA CGCTGGCCTT CAGCAGCATG 
ATGCCAGGCT ACAGACACGT GTACCTAGAA GGGATGGAAG AGGCCTCCAT CTTCGTGCAT 
GTGGCTGTCA GTGACATCAG CGGTAAGGTC AAGCAGGCTC TGGGCCTAAA AGGCCTCTTC 
CTCCGAGGCC CAAAGCCCGG CTCGCTGGAC AGTCATGCTG CTGGGCGGCC CCCGGCCCGG 
CCCTCCGTTA GCCAGCGGAT CCTGCGGCGC ACGGCCAGCG CCCCGACCAA GAGCCAGAAG 
CCGGGCCGCA GGGGCTTCCC GGAGCTGGTC CTGGGTACAC GGGACACAGG CTCCAAGGGG 
GTGGCAGACG ATGTGGTGCC CCCCGGGCCC GGACCTGCTC CGGAAGCCCC AGCCCAGGAG 
GGGCCCGGCA GCGGCAGCCC CCGAGGTAAG GCGCCAGCTG CGGTGGCAGA GAAGAGCCCT 
GTGCGAGTGC GGCCCCCGCG TGTCCTGGAC GGCCCCGGGC CTGCTGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG ATCCTGCGCC GGCGTGAACA CCGGGGGCCT GCAGAGGGAG 
CGGCCACCCA GCCCGGGGCC TGCAAGCAGG CAGGCAGCCA TTCGCCAGCA GCCCCGGGCC 
CGGGCTGACT CACTGGGGGC CCCCTGCTGT GGCCTGGACC CTCACGCTAT CCCGGGGAGA 
AGCAGAGAGG CCCCCAAGGG TCCTGGGGCC TGGAGGCAGG GTCCAGGCGG TAGCGGCTCC 
ATGTCCTCGG ACTCCAGCAG CCCAGACAGC CCGGGCATCC CCGAAAGGTC CCCCCGCTGG 
CCTGAGGGTG CCTGCAGGCA ACCGGGGGCC CTGCAGGGAG AGATGAGTGC CTTGTTTGCT 
CAAAAGCTGG AGGAGATCAG GAGTAAATCC CCCATGTTCT CCGCCGACAC CCGCCCCCTC 
TCCACGCAGC GGCCACTCCC CCCACTGTGC AGCCTGGAAA CCATCGCTGA GGAGCCCGCC 
CCAGGCCCTG GTCCCCCGCC ACCAGCGGCT GTCCCCACCA GCTCTTCTCA GGGACGGCCC 
CCATACCCCA CAGGACCCGG AGCCAATGTG GCAAGCCCCC TAGAGGACAC TGAGGAGCCC 
CGAGACAGCA GGCCTCGGCC GTGCAACGGC GAGGGCGCCG GCGGGGCATA CGAGAGGGCC 
CCCGGCAGCC AGACGGACGG CAGGAGCCAG CCCCGGACCC TGGGCCACCT GCCCGTGATT 
AGAAGGGTGA AGAGTGAGGG GCAGGTGCCC ACGGAGCCCC TGGGAGGGTG GCGGCCCCTG 
GCCGCTCCCT TTCCAGCTCC TGCCGTGTAC TCCGATGCCA CGGGCAGTGA CCCGCTGTGG 
CAGCGGCTGG AGCCATGTGG CCACCGAGAC AGCGTTTCCT CCTCCTCCAG CATGTCATCC 
AGCGACACTG TCATTGACCT CTCCCTGCCC AGCCTGGGCC TGGGCCGCAG CCGTGAGAAC 
CTCGCTGGAG CCCACATGGG ACGCCTGCCC CCCAGGCCCC ACTCGGCTTC GGCTGCCCGC 
CCAGACCTGC CACCTGTGAC CAAGAGCAAA TCCAACCCCA ACCTTCGGGC TACAGGCCAG 
CGGCCTCCCA TACCTGACGA ACTGCAGCCC AGGTCCCTGG CCCCAAGGAT GGCTGGCCTC 
CCCTTCCGGC CTCCCTGGGG CTGCCTTTCC CTGGTGGGCG TGCAGGACTG CCCCGTGGCT 
GCCAAGTCCA AGAGCCTGGG CGACCTCACT GCTGATGACT TTGCCCCTAG CTTTGAGGGC 
GGCTCCCGCA GACTGAGCCA CAGCCTGGGC CTCCCGGGAG GGACACGGCG GGTGTCGGGG 
CCAGGGGTGA GACGGGACAC CCTGACAGAG CAGCTGCGCT GGCTCACTGT CTTCCAGCAG 
GCAGGAGACA TCACGTCACC CACCAGCCTG GGCCCGGCTG GGGAGGGGGT GGCAGGGGGC 
CCTGGTTTTG TGCGGCGCTC CTCCTCCCGC AGCCACAGCC GCGTGCGTGC CATTGCCAGC 
CGGGCCCGCC AGGCCCAGGA GCGGCAGCAG AGACTGCAGG GCCTGGGCCG GCAGGGACCC 
CCAGAAGAGG AGCGGGGCAC CCCCGAGGGC GCCTGCTCCG TGGGCCACGA GGGCAGTGTG 
GATGCACCAG CACCCTCCAA GGGAGCCCTC GGGCCAGCAT CCGCGGCTGC TGAAAACCTG 
GTCCTGCTCC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_017002870.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858359.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu90824
Clone ID Related Accession (Same CDS sequence)	XM_017002870.1
Accession Version	XM_017002870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGGTAAGG CGCCAGCTGC GGTGGCAGAG AAGAGCCCTG TGCGAGTGCG GCCCCCGCGT 
GTCCTGGACG GCCCCGGGCC TGCTGGGATG GCCGCCACAT GCATGAAGTG TGTGGTGGGA 
TCCTGCGCCG GCGTGAACAC CGGGGGCCTG CAGAGGGAGC GGCCACCCAG CCCGGGGCCT 
GCAAGCAGGC AGGCAGCCAT TCGCCAGCAG CCCCGGGCCC GGGCTGACTC ACTGGGGGCC 
CCCTGCTGTG GCCTGGACCC TCACGCTATC CCGGGGAGAA GCAGAGAGGC CCCCAAGGGT 
CCTGGGGCCT GGAGGCAGGG TCCAGGCGGT AGCGGCTCCA TGTCCTCGGA CTCCAGCAGC 
CCAGACAGCC CGGGCATCCC CGAAAGGTCC CCCCGCTGGC CTGAGGGTGC CTGCAGGCAA 
CCGGGGGCCC TGCAGGGAGA GATGAGTGCC TTGTTTGCTC AAAAGCTGGA GGAGATCAGG 
AGTAAATCCC CCATGTTCTC CGCCGACACC CGCCCCCTCT CCACGCAGCG GCCACTCCCC 
CCACTGTGCA GCCTGGAAAC CATCGCTGAG GAGCCCGCCC CAGGCCCTGG TCCCCCGCCA 
CCAGCGGCTG TCCCCACCAG CTCTTCTCAG GGACGGCCCC CATACCCCAC AGGACCCGGA 
GCCAATGTGG CAAGCCCCCT AGAGGACACT GAGGAGCCCC GAGACAGCAG GCCTCGGCCG 
TGCAACGGCG AGGGCGCCGG CGGGGCATAC GAGAGGGCCC CCGGCAGCCA GACGGACGGC 
AGGAGCCAGC CCCGGACCCT GGGCCACCTG CCCGTGATTA GAAGGGTGAA GAGTGAGGGG 
CAGGTGCCCA CGGAGCCCCT GGGAGGGTGG CGGCCCCTGG CCGCTCCCTT TCCAGCTCCT 
GCCGTGTACT CCGATGCCAC GGGCAGTGAC CCGCTGTGGC AGCGGCTGGA GCCATGTGGC 
CACCGAGACA GCGTTTCCTC CTCCTCCAGC ATGTCATCCA GCGACACTGT CATTGACCTC 
TCCCTGCCCA GCCTGGGCCT GGGCCGCAGC CGTGAGAACC TCGCTGGAGC CCACATGGGA 
CGCCTGCCCC CCAGGCCCCA CTCGGCTTCG GCTGCCCGCC CAGACCTGCC ACCTGTGACC 
AAGAGCAAAT CCAACCCCAA CCTTCGGGCT ACAGGCCAGC GGCCTCCCAT ACCTGACGAA 
CTGCAGCCCA GGTCCCTGGC CCCAAGGATG GCTGGCCTCC CCTTCCGGCC TCCCTGGGGC 
TGCCTTTCCC TGGTGGGCGT GCAGGACTGC CCCGTGGCTG CCAAGTCCAA GAGCCTGGGC 
GACCTCACTG CTGATGACTT TGCCCCTAGC TTTGAGGGCG GCTCCCGCAG ACTGAGCCAC 
AGCCTGGGCC TCCCGGGAGG GACACGGCGG GTGTCGGGGC CAGGGGTGAG ACGGGACACC 
CTGACAGAGC AGCTGCGCTG GCTCACTGTC TTCCAGCAGG CAGGAGACAT CACGTCACCC 
ACCAGCCTGG GCCCGGCTGG GGAGGGGGTG GCAGGGGGCC CTGGTTTTGT GCGGCGCTCC 
TCCTCCCGCA GCCACAGCCG CGTGCGTGCC ATTGCCAGCC GGGCCCGCCA GGCCCAGGAG 
CGGCAGCAGA GACTGCAGGG CCTGGGCCGG CAGGGACCCC CAGAAGAGGA GCGGGGCACC 
CCCGAGGGCG CCTGCTCCGT GGGCCACGAG GGCAGTGTGG ATGCACCAGC ACCCTCCAAG 
GGAGCCCTCG GGCCAGCATC CGCGGCTGCT GAAAACCTGG TCCTGCTCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858359.1
CDS	21..5057
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_017002870.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002870.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGGTAAGG CGCCAGCTGC GGTGGCAGAG AAGAGCCCTG TGCGAGTGCG GCCCCCGCGT 
GTCCTGGACG GCCCCGGGCC TGCTGGGATG GCCGCCACAT GCATGAAGTG TGTGGTGGGA 
TCCTGCGCCG GCGTGAACAC CGGGGGCCTG CAGAGGGAGC GGCCACCCAG CCCGGGGCCT 
GCAAGCAGGC AGGCAGCCAT TCGCCAGCAG CCCCGGGCCC GGGCTGACTC ACTGGGGGCC 
CCCTGCTGTG GCCTGGACCC TCACGCTATC CCGGGGAGAA GCAGAGAGGC CCCCAAGGGT 
CCTGGGGCCT GGAGGCAGGG TCCAGGCGGT AGCGGCTCCA TGTCCTCGGA CTCCAGCAGC 
CCAGACAGCC CGGGCATCCC CGAAAGGTCC CCCCGCTGGC CTGAGGGTGC CTGCAGGCAA 
CCGGGGGCCC TGCAGGGAGA GATGAGTGCC TTGTTTGCTC AAAAGCTGGA GGAGATCAGG 
AGTAAATCCC CCATGTTCTC CGCCGACACC CGCCCCCTCT CCACGCAGCG GCCACTCCCC 
CCACTGTGCA GCCTGGAAAC CATCGCTGAG GAGCCCGCCC CAGGCCCTGG TCCCCCGCCA 
CCAGCGGCTG TCCCCACCAG CTCTTCTCAG GGACGGCCCC CATACCCCAC AGGACCCGGA 
GCCAATGTGG CAAGCCCCCT AGAGGACACT GAGGAGCCCC GAGACAGCAG GCCTCGGCCG 
TGCAACGGCG AGGGCGCCGG CGGGGCATAC GAGAGGGCCC CCGGCAGCCA GACGGACGGC 
AGGAGCCAGC CCCGGACCCT GGGCCACCTG CCCGTGATTA GAAGGGTGAA GAGTGAGGGG 
CAGGTGCCCA CGGAGCCCCT GGGAGGGTGG CGGCCCCTGG CCGCTCCCTT TCCAGCTCCT 
GCCGTGTACT CCGATGCCAC GGGCAGTGAC CCGCTGTGGC AGCGGCTGGA GCCATGTGGC 
CACCGAGACA GCGTTTCCTC CTCCTCCAGC ATGTCATCCA GCGACACTGT CATTGACCTC 
TCCCTGCCCA GCCTGGGCCT GGGCCGCAGC CGTGAGAACC TCGCTGGAGC CCACATGGGA 
CGCCTGCCCC CCAGGCCCCA CTCGGCTTCG GCTGCCCGCC CAGACCTGCC ACCTGTGACC 
AAGAGCAAAT CCAACCCCAA CCTTCGGGCT ACAGGCCAGC GGCCTCCCAT ACCTGACGAA 
CTGCAGCCCA GGTCCCTGGC CCCAAGGATG GCTGGCCTCC CCTTCCGGCC TCCCTGGGGC 
TGCCTTTCCC TGGTGGGCGT GCAGGACTGC CCCGTGGCTG CCAAGTCCAA GAGCCTGGGC 
GACCTCACTG CTGATGACTT TGCCCCTAGC TTTGAGGGCG GCTCCCGCAG ACTGAGCCAC 
AGCCTGGGCC TCCCGGGAGG GACACGGCGG GTGTCGGGGC CAGGGGTGAG ACGGGACACC 
CTGACAGAGC AGCTGCGCTG GCTCACTGTC TTCCAGCAGG CAGGAGACAT CACGTCACCC 
ACCAGCCTGG GCCCGGCTGG GGAGGGGGTG GCAGGGGGCC CTGGTTTTGT GCGGCGCTCC 
TCCTCCCGCA GCCACAGCCG CGTGCGTGCC ATTGCCAGCC GGGCCCGCCA GGCCCAGGAG 
CGGCAGCAGA GACTGCAGGG CCTGGGCCGG CAGGGACCCC CAGAAGAGGA GCGGGGCACC 
CCCGAGGGCG CCTGCTCCGT GGGCCACGAG GGCAGTGTGG ATGCACCAGC ACCCTCCAAG 
GGAGCCCTCG GGCCAGCATC CGCGGCTGCT GAAAACCTGG TCCTGCTCCG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_017002871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858360.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

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CloneID	OHu90825
Clone ID Related Accession (Same CDS sequence)	XM_017002871.1
Accession Version	XM_017002871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGG AGCAGGCCGG GGCTTGTGGG GGCCTCCGGT ACCTCTGCTG GGAGCTCGCG 
ATCCAAGGGA CACAGCGTGA GGGGCCACCG CAGGTGTGTG ATGCGCTGCT GGCCCAGGCT 
ACACCCCGAC AAGGGACACC GGGGGCCCCG GGAGCAGAGA GACCTCAGAG CAGCCTCCTC 
CTGCCTCCTG TGGACGGCCG GCCCCAGCTG GTGATCCCAG CCAGTCCCAG CTTTCAGTTG 
CTGCCCCCAC CGACAGTCCT CAGTCCCTCC ATGATGGCTC CCCCGACAGC CGGCCCCCTT 
CCTGGCCCAG CTCTTCCGCC TGAGGACCCA GGGCCGGATC CGGAGAGCAG GTGGCTTTTC 
TTGAGCGCCA ACATTCTGCC CGTGGTGGAG CGGTGCATGG GTGCCATGCA AGAGGGGATG 
CAGATGGTGA AGCTGCGTGG CGGCTCCAAG GGCCTGGTCC GCTTCTACTA CCTGGACGAG 
CACCGCTCCT GCATCCGCTG GAGGCCCTCA CGCAAGAACG AGAAGGCCAA GATCTCCATC 
GACTCCATCC AGGAGGTGAG TGAGGGGCGG CAGTCGGAGG TCTTCCAGCG CTACCCTGAC 
GGCAGCTTCG ACCCCAACTG CTGCTTCAGC ATCTACCACG GCAGCCACCG CGAGTCGCTG 
GACCTGGTCT CCACCAGCAG CGAGGTGGCG CGCACCTGGG TCACTGGCCT GCGCTACCTC 
ATGGCCGGCA TCAGCGACGA GGACAGCCTG GCTCGCCGCC AGCGCACCAG GGACCAGTGG 
CTGAAGCAGA CGTTTGACGA GGCCGACAAG AACGGGGATG GCAGCCTGAG CATTGGCGAG 
GTCCTGCAGC TGCTGCACAA GCTCAACGTG AACCTGCCCC GGCAGAGGGT GAAGCAGATG 
TTCAGGGAAG CGGACACGGA TGACCACCAA GGGACGCTGG GTTTTGAAGA GTTCTGTGCC 
TTCTACAAGA TGATGTCCAC CCGCCGGGAC CTCTACCTGC TCATGCTGAC CTACAGCAAC 
CACAAGGACC ACCTGGATGC CGCCAGCCTG CAGCGCTTCC TGCAGGTGGA GCAGAAGATG 
GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC ATCATCGAGC AGTTTGAGCC ATGCCCAGAA 
AACAAGAGTA AGGGGCTGCT GGGCATTGAT GGCTTCACCA ACTACACCAG GAGCCCTGCT 
GGTGACATCT TCAACCCTGA GCACCACCAT GTGCACCAGG ACATGACGCA GCCGCTGAGC 
CACTACTTCA TCACCTCGTC CCACAACACC TACCTCGTGG GTGACCAGCT CATGTCCCAG 
TCACGGGTGG ACATGTATGC TTGGGTCCTG CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC 
TGCTGGGATG GGCCCGACGG GGAGCCCATT GTGCACCATG GCTACACTCT GACTTCCAAG 
ATCCTCTTCA AAGACGTCAT TGAAACCATC AACAAATATG CCTTCATCAA GAATGAGTAC 
CCAGTGATCC TGTCCATCGA AAACCACTGC AGTGTCATCC AGCAGAAGAA AATGGCCCAG 
TATCTGACTG ACATCCTTGG GGACAAGCTG GACCTGTCAT CAGTGAGCAG TGAAGATGCC 
ACCACACTCC CCTCTCCACA GATGCTCAAG GGCAAGATCC TCGTGAAGGG GAAGAAGCTC 
CCAGCCAACA TCAGCGAGGA TGCGGAGGAA GGCGAGGTGT CTGATGAGGA CAGTGCTGAT 
GAGATTGACG ATGACTGCAA GCTCCTCAAT GGGGATGCAT CCACCAATCG AAAGCGTGTA 
GAAAACACTG CTAAGAGGAA ACTGGATTCC CTCATCAAAG AGTCGAAGAT TCGGGACTGT 
GAGGACCCCA ACAACTTCTC CGTCTCCACA CTGTCCCCAT CTGGAAAGCT CGGACGCAAG 
AGCAAGGCTG AAGAGGACGT GGAGTCTGGG GAGGATGCCG GGGCCAGCAG ACGCAATGGC 
CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG 
GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG GACTCCCCGG GAGGCCAGAG CCGAGGGGCG 
ACCCGGCAGA AGAAGACCAT GAAGCTGTCC CGGGCCCTCT CTGACCTGGT GAAGTACACC 
AAGTCCGTGG CCACCCACGA CATAGAGATG GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC 
TTCAGCGAGA CCAAGGCCCA CCAGATTCTG CAGCAGAAGC CGGCGCAGTA CCTACGCTTC 
AACCAGCAGC AGCTCTCCCG CATCTACCCC TCCTCCTACC GTGTGGACTC CAGCAACTAC 
AACCCGCAGC CCTTCTGGAA CGCCGGCTGC CAAATGGTTG CCCTGAACTA CCAGTCAGAG 
GGGCGGATGC TGCAGCTGAA CCGAGCCAAG TTCAGCGCCA ACGGTGGCTG CGGCTACGTA 
CTCAAGCCTG GGTGCATGTG CCAGGGCGTG TTCAACCCCA ACTCGGAGGA CCCCCTGCCC 
GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG ATCATCAGTG GCCAGCAGCT TCCCAAGCCG 
CGCGACTCCA TGCTGGGGGA CCGTGGGGAG ATCATCGACC CCTTTGTGGA GGTGGAGATC 
ATTGGGCTCC CTGTGGACTG CAGCAGGGAG CAGACCCGCG TGGTGGACGA CAACGGGTTC 
AACCCCACCT GGGAGGAGAC CCTGGTTTTC ATGGTGCACA TGCCGGAGAT CGCGCTGGTC 
CGCTTCCTCG TCTGGGACCA CGATCCCATC GGGCGTGACT TCATTGGCCA GAGGACGCTG 
GCCTTCAGCA GCATGATGCC AGGCTACAGA CACGTGTACC TAGAAGGGAT GGAAGAGGCC 
TCCATCTTCG TGCATGTGGC TGTCAGTGAC ATCAGCGGTA AGGTCAAGCA GGCTCTGGGC 
CTAAAAGGCC TCTTCCTCCG AGGCCCAAAG CCCGGCTCGC TGGACAGTCA TGCTGCTGGG 
CGGCCCCCGG CCCGGCCCTC CGTTAGCCAG CGGATCCTGC GGCGCACGGC CAGCGCCCCG 
ACCAAGAGCC AGAAGCCGGG CCGCAGGGGC TTCCCGGAGC TGGTCCTGGG TACACGGGAC 
ACAGGCTCCA AGGGGGTGGC AGACGATGTG GTGCCCCCCG GGCCCGGACC TGCTCCGGAA 
GCCCCAGCCC AGGAGGGGCC CGGCAGCGGC AGCCCCCGAG GTAAGGCGCC AGCTGCGGTG 
GCAGAGAAGA GCCCTGTGCG AGTGCGGCCC CCGCGTGTCC TGGACGGCCC CGGGCCTGCT 
GGGATGGCCG CCACATGCAT GAAGTGTGTG GTGGGATCCT GCGCCGGCGT GAACACCGGG 
GGCCTGCAGA GGGAGCGGCC ACCCAGCCCG GGGCCTGCAA GCAGGCAGGC AGCCATTCGC 
CAGCAGCCCC GGGCCCGGGC TGACTCACTG GGGGCCCCCT GCTGTGGCCT GGACCCTCAC 
GCTATCCCGG GGAGAAGCAG AGAGGCCCCC AAGGGTCCTG GGGCCTGGAG GCAGGGTCCA 
GGCGGTAGCG GCTCCATGTC CTCGGACTCC AGCAGCCCAG ACAGCCCGGG CATCCCCGAA 
AGGTCCCCCC GCTGGCCTGA GGGTGCCTGC AGGCAACCGG GGGCCCTGCA GGGAGAGATG 
AGTGCCTTGT TTGCTCAAAA GCTGGAGGAG ATCAGGAGTA AATCCCCCAT GTTCTCCGCC 
GACACCCGCC CCCTCTCCAC GCAGCGGCCA CTCCCCCCAC TGTGCAGCCT GGAAACCATC 
GCTGAGGAGC CCGCCCCAGG CCCTGGTCCC CCGCCACCAG CGGCTGTCCC CACCAGCTCT 
TCTCAGGGAC GGCCCCCATA CCCCACAGGA CCCGGAGCCA ATGTGGCAAG CCCCCTAGAG 
GACACTGAGG AGCCCCGAGA CAGCAGGCCT CGGCCGTGCA ACGGCGAGGG CGCCGGCGGG 
GCATACGAGA GGGCCCCCGG CAGCCAGACG GACGGCAGGA GCCAGCCCCG GACCCTGGGC 
CACCTGCCCG TGATTAGAAG GGTGAAGAGT GAGGGGCAGG TGCCCACGGA GCCCCTGGGA 
GGGTGGCGGC CCCTGGCCGC TCCCTTTCCA GCTCCTGCCG TGTACTCCGA TGCCACGGGC 
AGTGACCCGC TGTGGCAGCG GCTGGAGCCA TGTGGCCACC GAGACAGCGT TTCCTCCTCC 
TCCAGCATGT CATCCAGCGA CACTGTCATT GACCTCTCCC TGCCCAGCCT GGGCCTGGGC 
CGCAGCCGTG AGAACCTCGC TGGAGCCCAC ATGGGACGCC TGCCCCCCAG GCCCCACTCG 
GCTTCGGCTG CCCGCCCAGA CCTGCCACCT GTGACCAAGA GCAAATCCAA CCCCAACCTT 
CGGGCTACAG GCCAGCGGCC TCCCATACCT GACGAACTGC AGCCCAGGTC CCTGGCCCCA 
AGGATGGCTG GCCTCCCCTT CCGGCCTCCC TGGGGCTGCC TTTCCCTGGT GGGCGTGCAG 
GACTGCCCCG TGGCTGCCAA GTCCAAGAGC CTGGGCGACC TCACTGCTGA TGACTTTGCC 
CCTAGCTTTG AGGGCGGCTC CCGCAGACTG AGCCACAGCC TGGGCCTCCC GGGAGGGACA 
CGGCGGGTGT CGGGGCCAGG GGTGAGACGG GACACCCTGA CAGAGCAGCT GCGCTGGCTC 
ACTGTCTTCC AGCAGGCAGG AGACATCACG TCACCCACCA GCCTGGGCCC GGCTGGGGAG 
GGGGTGGCAG GGGGCCCTGG TTTTGTGCGG CGCTCCTCCT CCCGCAGCCA CAGCCGCGTG 
CGTGCCATTG CCAGCCGGGC CCGCCAGGCC CAGGAGCGGC AGCAGAGACT GCAGGGCCTG 
GGCCGGCAGG GACCCCCAGA AGAGGAGCGG GGCACCCCCG AGGGCGCCTG CTCCGTGGGC 
CACGAGGGCA GTGTGGATGC ACCAGCACCC TCCAAGGGAG CCCTCGGGCC AGCATCCGCG 
GCTGCTGAAA ACCTGGTCCT GCTCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858360.1
CDS	16..5028
Translation	MSEEQAGACGGLRYLCWELAIQGTQREGPPQVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_017002871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002871.1

ORF Insert Sequence:

ATGTCAGAGG AGCAGGCCGG GGCTTGTGGG GGCCTCCGGT ACCTCTGCTG GGAGCTCGCG 
ATCCAAGGGA CACAGCGTGA GGGGCCACCG CAGGTGTGTG ATGCGCTGCT GGCCCAGGCT 
ACACCCCGAC AAGGGACACC GGGGGCCCCG GGAGCAGAGA GACCTCAGAG CAGCCTCCTC 
CTGCCTCCTG TGGACGGCCG GCCCCAGCTG GTGATCCCAG CCAGTCCCAG CTTTCAGTTG 
CTGCCCCCAC CGACAGTCCT CAGTCCCTCC ATGATGGCTC CCCCGACAGC CGGCCCCCTT 
CCTGGCCCAG CTCTTCCGCC TGAGGACCCA GGGCCGGATC CGGAGAGCAG GTGGCTTTTC 
TTGAGCGCCA ACATTCTGCC CGTGGTGGAG CGGTGCATGG GTGCCATGCA AGAGGGGATG 
CAGATGGTGA AGCTGCGTGG CGGCTCCAAG GGCCTGGTCC GCTTCTACTA CCTGGACGAG 
CACCGCTCCT GCATCCGCTG GAGGCCCTCA CGCAAGAACG AGAAGGCCAA GATCTCCATC 
GACTCCATCC AGGAGGTGAG TGAGGGGCGG CAGTCGGAGG TCTTCCAGCG CTACCCTGAC 
GGCAGCTTCG ACCCCAACTG CTGCTTCAGC ATCTACCACG GCAGCCACCG CGAGTCGCTG 
GACCTGGTCT CCACCAGCAG CGAGGTGGCG CGCACCTGGG TCACTGGCCT GCGCTACCTC 
ATGGCCGGCA TCAGCGACGA GGACAGCCTG GCTCGCCGCC AGCGCACCAG GGACCAGTGG 
CTGAAGCAGA CGTTTGACGA GGCCGACAAG AACGGGGATG GCAGCCTGAG CATTGGCGAG 
GTCCTGCAGC TGCTGCACAA GCTCAACGTG AACCTGCCCC GGCAGAGGGT GAAGCAGATG 
TTCAGGGAAG CGGACACGGA TGACCACCAA GGGACGCTGG GTTTTGAAGA GTTCTGTGCC 
TTCTACAAGA TGATGTCCAC CCGCCGGGAC CTCTACCTGC TCATGCTGAC CTACAGCAAC 
CACAAGGACC ACCTGGATGC CGCCAGCCTG CAGCGCTTCC TGCAGGTGGA GCAGAAGATG 
GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC ATCATCGAGC AGTTTGAGCC ATGCCCAGAA 
AACAAGAGTA AGGGGCTGCT GGGCATTGAT GGCTTCACCA ACTACACCAG GAGCCCTGCT 
GGTGACATCT TCAACCCTGA GCACCACCAT GTGCACCAGG ACATGACGCA GCCGCTGAGC 
CACTACTTCA TCACCTCGTC CCACAACACC TACCTCGTGG GTGACCAGCT CATGTCCCAG 
TCACGGGTGG ACATGTATGC TTGGGTCCTG CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC 
TGCTGGGATG GGCCCGACGG GGAGCCCATT GTGCACCATG GCTACACTCT GACTTCCAAG 
ATCCTCTTCA AAGACGTCAT TGAAACCATC AACAAATATG CCTTCATCAA GAATGAGTAC 
CCAGTGATCC TGTCCATCGA AAACCACTGC AGTGTCATCC AGCAGAAGAA AATGGCCCAG 
TATCTGACTG ACATCCTTGG GGACAAGCTG GACCTGTCAT CAGTGAGCAG TGAAGATGCC 
ACCACACTCC CCTCTCCACA GATGCTCAAG GGCAAGATCC TCGTGAAGGG GAAGAAGCTC 
CCAGCCAACA TCAGCGAGGA TGCGGAGGAA GGCGAGGTGT CTGATGAGGA CAGTGCTGAT 
GAGATTGACG ATGACTGCAA GCTCCTCAAT GGGGATGCAT CCACCAATCG AAAGCGTGTA 
GAAAACACTG CTAAGAGGAA ACTGGATTCC CTCATCAAAG AGTCGAAGAT TCGGGACTGT 
GAGGACCCCA ACAACTTCTC CGTCTCCACA CTGTCCCCAT CTGGAAAGCT CGGACGCAAG 
AGCAAGGCTG AAGAGGACGT GGAGTCTGGG GAGGATGCCG GGGCCAGCAG ACGCAATGGC 
CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG 
GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG GACTCCCCGG GAGGCCAGAG CCGAGGGGCG 
ACCCGGCAGA AGAAGACCAT GAAGCTGTCC CGGGCCCTCT CTGACCTGGT GAAGTACACC 
AAGTCCGTGG CCACCCACGA CATAGAGATG GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC 
TTCAGCGAGA CCAAGGCCCA CCAGATTCTG CAGCAGAAGC CGGCGCAGTA CCTACGCTTC 
AACCAGCAGC AGCTCTCCCG CATCTACCCC TCCTCCTACC GTGTGGACTC CAGCAACTAC 
AACCCGCAGC CCTTCTGGAA CGCCGGCTGC CAAATGGTTG CCCTGAACTA CCAGTCAGAG 
GGGCGGATGC TGCAGCTGAA CCGAGCCAAG TTCAGCGCCA ACGGTGGCTG CGGCTACGTA 
CTCAAGCCTG GGTGCATGTG CCAGGGCGTG TTCAACCCCA ACTCGGAGGA CCCCCTGCCC 
GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG ATCATCAGTG GCCAGCAGCT TCCCAAGCCG 
CGCGACTCCA TGCTGGGGGA CCGTGGGGAG ATCATCGACC CCTTTGTGGA GGTGGAGATC 
ATTGGGCTCC CTGTGGACTG CAGCAGGGAG CAGACCCGCG TGGTGGACGA CAACGGGTTC 
AACCCCACCT GGGAGGAGAC CCTGGTTTTC ATGGTGCACA TGCCGGAGAT CGCGCTGGTC 
CGCTTCCTCG TCTGGGACCA CGATCCCATC GGGCGTGACT TCATTGGCCA GAGGACGCTG 
GCCTTCAGCA GCATGATGCC AGGCTACAGA CACGTGTACC TAGAAGGGAT GGAAGAGGCC 
TCCATCTTCG TGCATGTGGC TGTCAGTGAC ATCAGCGGTA AGGTCAAGCA GGCTCTGGGC 
CTAAAAGGCC TCTTCCTCCG AGGCCCAAAG CCCGGCTCGC TGGACAGTCA TGCTGCTGGG 
CGGCCCCCGG CCCGGCCCTC CGTTAGCCAG CGGATCCTGC GGCGCACGGC CAGCGCCCCG 
ACCAAGAGCC AGAAGCCGGG CCGCAGGGGC TTCCCGGAGC TGGTCCTGGG TACACGGGAC 
ACAGGCTCCA AGGGGGTGGC AGACGATGTG GTGCCCCCCG GGCCCGGACC TGCTCCGGAA 
GCCCCAGCCC AGGAGGGGCC CGGCAGCGGC AGCCCCCGAG GTAAGGCGCC AGCTGCGGTG 
GCAGAGAAGA GCCCTGTGCG AGTGCGGCCC CCGCGTGTCC TGGACGGCCC CGGGCCTGCT 
GGGATGGCCG CCACATGCAT GAAGTGTGTG GTGGGATCCT GCGCCGGCGT GAACACCGGG 
GGCCTGCAGA GGGAGCGGCC ACCCAGCCCG GGGCCTGCAA GCAGGCAGGC AGCCATTCGC 
CAGCAGCCCC GGGCCCGGGC TGACTCACTG GGGGCCCCCT GCTGTGGCCT GGACCCTCAC 
GCTATCCCGG GGAGAAGCAG AGAGGCCCCC AAGGGTCCTG GGGCCTGGAG GCAGGGTCCA 
GGCGGTAGCG GCTCCATGTC CTCGGACTCC AGCAGCCCAG ACAGCCCGGG CATCCCCGAA 
AGGTCCCCCC GCTGGCCTGA GGGTGCCTGC AGGCAACCGG GGGCCCTGCA GGGAGAGATG 
AGTGCCTTGT TTGCTCAAAA GCTGGAGGAG ATCAGGAGTA AATCCCCCAT GTTCTCCGCC 
GACACCCGCC CCCTCTCCAC GCAGCGGCCA CTCCCCCCAC TGTGCAGCCT GGAAACCATC 
GCTGAGGAGC CCGCCCCAGG CCCTGGTCCC CCGCCACCAG CGGCTGTCCC CACCAGCTCT 
TCTCAGGGAC GGCCCCCATA CCCCACAGGA CCCGGAGCCA ATGTGGCAAG CCCCCTAGAG 
GACACTGAGG AGCCCCGAGA CAGCAGGCCT CGGCCGTGCA ACGGCGAGGG CGCCGGCGGG 
GCATACGAGA GGGCCCCCGG CAGCCAGACG GACGGCAGGA GCCAGCCCCG GACCCTGGGC 
CACCTGCCCG TGATTAGAAG GGTGAAGAGT GAGGGGCAGG TGCCCACGGA GCCCCTGGGA 
GGGTGGCGGC CCCTGGCCGC TCCCTTTCCA GCTCCTGCCG TGTACTCCGA TGCCACGGGC 
AGTGACCCGC TGTGGCAGCG GCTGGAGCCA TGTGGCCACC GAGACAGCGT TTCCTCCTCC 
TCCAGCATGT CATCCAGCGA CACTGTCATT GACCTCTCCC TGCCCAGCCT GGGCCTGGGC 
CGCAGCCGTG AGAACCTCGC TGGAGCCCAC ATGGGACGCC TGCCCCCCAG GCCCCACTCG 
GCTTCGGCTG CCCGCCCAGA CCTGCCACCT GTGACCAAGA GCAAATCCAA CCCCAACCTT 
CGGGCTACAG GCCAGCGGCC TCCCATACCT GACGAACTGC AGCCCAGGTC CCTGGCCCCA 
AGGATGGCTG GCCTCCCCTT CCGGCCTCCC TGGGGCTGCC TTTCCCTGGT GGGCGTGCAG 
GACTGCCCCG TGGCTGCCAA GTCCAAGAGC CTGGGCGACC TCACTGCTGA TGACTTTGCC 
CCTAGCTTTG AGGGCGGCTC CCGCAGACTG AGCCACAGCC TGGGCCTCCC GGGAGGGACA 
CGGCGGGTGT CGGGGCCAGG GGTGAGACGG GACACCCTGA CAGAGCAGCT GCGCTGGCTC 
ACTGTCTTCC AGCAGGCAGG AGACATCACG TCACCCACCA GCCTGGGCCC GGCTGGGGAG 
GGGGTGGCAG GGGGCCCTGG TTTTGTGCGG CGCTCCTCCT CCCGCAGCCA CAGCCGCGTG 
CGTGCCATTG CCAGCCGGGC CCGCCAGGCC CAGGAGCGGC AGCAGAGACT GCAGGGCCTG 
GGCCGGCAGG GACCCCCAGA AGAGGAGCGG GGCACCCCCG AGGGCGCCTG CTCCGTGGGC 
CACGAGGGCA GTGTGGATGC ACCAGCACCC TCCAAGGGAG CCCTCGGGCC AGCATCCGCG 
GCTGCTGAAA ACCTGGTCCT GCTCCGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_017002872.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858361.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.

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CloneID	OHu90826
Clone ID Related Accession (Same CDS sequence)	XM_017002872.1
Accession Version	XM_017002872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGACACCC GCCCCCTCTC CACGCAGCGG CCACTCCCCC CACTGTGCAG CCTGGAAACC 
ATCGCTGAGG AGCCCGCCCC AGGCCCTGGT CCCCCGCCAC CAGCGGCTGT CCCCACCAGC 
TCTTCTCAGG GACGGCCCCC ATACCCCACA GGACCCGGAG CCAATGTGGC AAGCCCCCTA 
GAGGACACTG AGGAGCCCCG AGACAGCAGG CCTCGGCCGT GCAACGGCGA GGGCGCCGGC 
GGGGCATACG AGAGGGCCCC CGGCAGCCAG ACGGACGGCA GGAGCCAGCC CCGGACCCTG 
GGCCACCTGC CCGTGATTAG AAGGGTGAAG AGTGAGGGGC AGGTGCCCAC GGAGCCCCTG 
GGAGGGTGGC GGCCCCTGGC CGCTCCCTTT CCAGCTCCTG CCGTGTACTC CGATGCCACG 
GGCAGTGACC CGCTGTGGCA GCGGCTGGAG CCATGTGGCC ACCGAGACAG CGTTTCCTCC 
TCCTCCAGCA TGTCATCCAG CGACACTGTC ATTGACCTCT CCCTGCCCAG CCTGGGCCTG 
GGCCGCAGCC GTGAGAACCT CGCTGGAGCC CACATGGGAC GCCTGCCCCC CAGGCCCCAC 
TCGGCTTCGG CTGCCCGCCC AGACCTGCCA CCTGTGACCA AGAGCAAATC CAACCCCAAC 
CTTCGGGCTA CAGGCCAGCG GCCTCCCATA CCTGACGAAC TGCAGCCCAG GTCCCTGGCC 
CCAAGGATGG CTGGCCTCCC CTTCCGGCCT CCCTGGGGCT GCCTTTCCCT GGTGGGCGTG 
CAGGACTGCC CCGTGGCTGC CAAGTCCAAG AGCCTGGGCG ACCTCACTGC TGATGACTTT 
GCCCCTAGCT TTGAGGGCGG CTCCCGCAGA CTGAGCCACA GCCTGGGCCT CCCGGGAGGG 
ACACGGCGGG TGTCGGGGCC AGGGGTGAGA CGGGACACCC TGACAGAGCA GCTGCGCTGG 
CTCACTGTCT TCCAGCAGGC AGGAGACATC ACGTCACCCA CCAGCCTGGG CCCGGCTGGG 
GAGGGGGTGG CAGGGGGCCC TGGTTTTGTG CGGCGCTCCT CCTCCCGCAG CCACAGCCGC 
GTGCGTGCCA TTGCCAGCCG GGCCCGCCAG GCCCAGGAGC GGCAGCAGAG ACTGCAGGGC 
CTGGGCCGGC AGGGACCCCC AGAAGAGGAG CGGGGCACCC CCGAGGGCGC CTGCTCCGTG 
GGCCACGAGG GCAGTGTGGA TGCACCAGCA CCCTCCAAGG GAGCCCTCGG GCCAGCATCC 
GCGGCTGCTG AAAACCTGGT CCTGCTCCGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858361.1
CDS	21..4556
Translation	MAAGSGGPEQPRRPRPRPDGRELMTARRGSACGADLCVKVCDALLAQATPRQGTPGAPGAERPQSSLLLPPVDGRPQLVIPASPSFQLLPPPTVLSPSMMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRDTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_017002872.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002872.1

ORF Insert Sequence:

ATGGCCGCGG GTTCGGGTGG TCCCGAGCAG CCACGCCGGC CACGCCCACG CCCAGACGGG 
CGAGAACTGA TGACGGCACG ACGTGGCTCT GCGTGCGGCG CAGACCTCTG CGTCAAGGTG 
TGTGATGCGC TGCTGGCCCA GGCTACACCC CGACAAGGGA CACCGGGGGC CCCGGGAGCA 
GAGAGACCTC AGAGCAGCCT CCTCCTGCCT CCTGTGGACG GCCGGCCCCA GCTGGTGATC 
CCAGCCAGTC CCAGCTTTCA GTTGCTGCCC CCACCGACAG TCCTCAGTCC CTCCATGATG 
GCTCCCCCGA CAGCCGGCCC CCTTCCTGGC CCAGCTCTTC CGCCTGAGGA CCCAGGGCCG 
GATCCGGAGA GCAGGTGGCT TTTCTTGAGC GCCAACATTC TGCCCGTGGT GGAGCGGTGC 
ATGGGTGCCA TGCAAGAGGG GATGCAGATG GTGAAGCTGC GTGGCGGCTC CAAGGGCCTG 
GTCCGCTTCT ACTACCTGGA CGAGCACCGC TCCTGCATCC GCTGGAGGCC CTCACGCAAG 
AACGAGAAGG CCAAGATCTC CATCGACTCC ATCCAGGAGG TGAGTGAGGG GCGGCAGTCG 
GAGGTCTTCC AGCGCTACCC TGACGGCAGC TTCGACCCCA ACTGCTGCTT CAGCATCTAC 
CACGGCAGCC ACCGCGAGTC GCTGGACCTG GTCTCCACCA GCAGCGAGGT GGCGCGCACC 
TGGGTCACTG GCCTGCGCTA CCTCATGGCC GGCATCAGCG ACGAGGACAG CCTGGCTCGC 
CGCCAGCGCA CCAGGGACCA GTGGCTGAAG CAGACGTTTG ACGAGGCCGA CAAGAACGGG 
GATGGCAGCC TGAGCATTGG CGAGGTCCTG CAGCTGCTGC ACAAGCTCAA CGTGAACCTG 
CCCCGGCAGA GGGTGAAGCA GATGTTCAGG GAAGCGGACA CGGATGACCA CCAAGGGACG 
CTGGGTTTTG AAGAGTTCTG TGCCTTCTAC AAGATGATGT CCACCCGCCG GGACCTCTAC 
CTGCTCATGC TGACCTACAG CAACCACAAG GACCACCTGG ATGCCGCCAG CCTGCAGCGC 
TTCCTGCAGG TGGAGCAGAA GATGGCGGGT GTGACCCTCG AGAGCTGCCA GGACATCATC 
GAGCAGTTTG AGCCATGCCC AGAAAACAAG AGTAAGGGGC TGCTGGGCAT TGATGGCTTC 
ACCAACTACA CCAGGAGCCC TGCTGGTGAC ATCTTCAACC CTGAGCACCA CCATGTGCAC 
CAGGACATGA CGCAGCCGCT GAGCCACTAC TTCATCACCT CGTCCCACAA CACCTACCTC 
GTGGGTGACC AGCTCATGTC CCAGTCACGG GTGGACATGT ATGCTTGGGT CCTGCAGGCT 
GGCTGCCGCT GCGTGGAGGT GGACTGCTGG GATGGGCCCG ACGGGGAGCC CATTGTGCAC 
CATGGCTACA CTCTGACTTC CAAGATCCTC TTCAAAGACG TCATTGAAAC CATCAACAAA 
TATGCCTTCA TCAAGAATGA GTACCCAGTG ATCCTGTCCA TCGAAAACCA CTGCAGTGTC 
ATCCAGCAGA AGAAAATGGC CCAGTATCTG ACTGACATCC TTGGGGACAA GCTGGACCTG 
TCATCAGTGA GCAGTGAAGA TGCCACCACA CTCCCCTCTC CACAGATGCT CAAGGGCAAG 
ATCCTCGTGA AGGGGAAGAA GCTCCCAGCC AACATCAGCG AGGATGCGGA GGAAGGCGAG 
GTGTCTGATG AGGACAGTGC TGATGAGATT GACGATGACT GCAAGCTCCT CAATGGGGAT 
GCATCCACCA ATCGAAAGCG TGTAGAAAAC ACTGCTAAGA GGAAACTGGA TTCCCTCATC 
AAAGAGTCGA AGATTCGGGA CTGTGAGGAC CCCAACAACT TCTCCGTCTC CACACTGTCC 
CCATCTGGAA AGCTCGGACG CAAGAGCAAG GCTGAAGAGG ACGTGGAGTC TGGGGAGGAT 
GCCGGGGCCA GCAGACGCAA TGGCCGCCTC GTCGTGGGAA GCTTCTCCAG GCGCAAGAAG 
AAGGGCAGCA AGCTGAAGAA GGCGGCCAGC GTGGAGGAGG GAGATGAGGG TCAGGACTCC 
CCGGGAGGCC AGAGCCGAGG GGCGACCCGG CAGAAGAAGA CCATGAAGCT GTCCCGGGCC 
CTCTCTGACC TGGTGAAGTA CACCAAGTCC GTGGCCACCC ACGACATAGA GATGGAGGCG 
GCGTCCAGCT GGCAGGTGTC GTCCTTCAGC GAGACCAAGG CCCACCAGAT TCTGCAGCAG 
AAGCCGGCGC AGTACCTACG CTTCAACCAG CAGCAGCTCT CCCGCATCTA CCCCTCCTCC 
TACCGTGTGG ACTCCAGCAA CTACAACCCG CAGCCCTTCT GGAACGCCGG CTGCCAAATG 
GTTGCCCTGA ACTACCAGTC AGAGGGGCGG ATGCTGCAGC TGAACCGAGC CAAGTTCAGC 
GCCAACGGTG GCTGCGGCTA CGTACTCAAG CCTGGGTGCA TGTGCCAGGG CGTGTTCAAC 
CCCAACTCGG AGGACCCCCT GCCCGGGCAG CTCAAGAAGC AGCTGGTGCT CCGGATCATC 
AGTGGCCAGC AGCTTCCCAA GCCGCGCGAC TCCATGCTGG GGGACCGTGG GGAGATCATC 
GACCCCTTTG TGGAGGTGGA GATCATTGGG CTCCCTGTGG ACTGCAGCAG GGAGCAGACC 
CGCGTGGTGG ACGACAACGG GTTCAACCCC ACCTGGGAGG AGACCCTGGT TTTCATGGTG 
CACATGCCGG AGATCGCGCT GGTCCGCTTC CTCGTCTGGG ACCACGATCC CATCGGGCGT 
GACTTCATTG GCCAGAGGAC GCTGGCCTTC AGCAGCATGA TGCCAGGCTA CAGACACGTG 
TACCTAGAAG GGATGGAAGA GGCCTCCATC TTCGTGCATG TGGCTGTCAG TGACATCAGC 
GGTAAGGTCA AGCAGGCTCT GGGCCTAAAA GGCCTCTTCC TCCGAGGCCC AAAGCCCGGC 
TCGCTGGACA GTCATGCTGC TGGGCGGCCC CCGGCCCGGC CCTCCGTTAG CCAGCGGATC 
CTGCGGCGCA CGGCCAGCGC CCCGACCAAG AGCCAGAAGC CGGGCCGCAG GGGCTTCCCG 
GAGCTGGTCC TGGGTACACG GGACACAGGC TCCAAGGGGG TGGCAGACGA TGTGGTGCCC 
CCCGGGCCCG GACCTGCTCC GGAAGCCCCA GCCCAGGAGG GGCCCGGCAG CGGCAGCCCC 
CGAGACACCC GCCCCCTCTC CACGCAGCGG CCACTCCCCC CACTGTGCAG CCTGGAAACC 
ATCGCTGAGG AGCCCGCCCC AGGCCCTGGT CCCCCGCCAC CAGCGGCTGT CCCCACCAGC 
TCTTCTCAGG GACGGCCCCC ATACCCCACA GGACCCGGAG CCAATGTGGC AAGCCCCCTA 
GAGGACACTG AGGAGCCCCG AGACAGCAGG CCTCGGCCGT GCAACGGCGA GGGCGCCGGC 
GGGGCATACG AGAGGGCCCC CGGCAGCCAG ACGGACGGCA GGAGCCAGCC CCGGACCCTG 
GGCCACCTGC CCGTGATTAG AAGGGTGAAG AGTGAGGGGC AGGTGCCCAC GGAGCCCCTG 
GGAGGGTGGC GGCCCCTGGC CGCTCCCTTT CCAGCTCCTG CCGTGTACTC CGATGCCACG 
GGCAGTGACC CGCTGTGGCA GCGGCTGGAG CCATGTGGCC ACCGAGACAG CGTTTCCTCC 
TCCTCCAGCA TGTCATCCAG CGACACTGTC ATTGACCTCT CCCTGCCCAG CCTGGGCCTG 
GGCCGCAGCC GTGAGAACCT CGCTGGAGCC CACATGGGAC GCCTGCCCCC CAGGCCCCAC 
TCGGCTTCGG CTGCCCGCCC AGACCTGCCA CCTGTGACCA AGAGCAAATC CAACCCCAAC 
CTTCGGGCTA CAGGCCAGCG GCCTCCCATA CCTGACGAAC TGCAGCCCAG GTCCCTGGCC 
CCAAGGATGG CTGGCCTCCC CTTCCGGCCT CCCTGGGGCT GCCTTTCCCT GGTGGGCGTG 
CAGGACTGCC CCGTGGCTGC CAAGTCCAAG AGCCTGGGCG ACCTCACTGC TGATGACTTT 
GCCCCTAGCT TTGAGGGCGG CTCCCGCAGA CTGAGCCACA GCCTGGGCCT CCCGGGAGGG 
ACACGGCGGG TGTCGGGGCC AGGGGTGAGA CGGGACACCC TGACAGAGCA GCTGCGCTGG 
CTCACTGTCT TCCAGCAGGC AGGAGACATC ACGTCACCCA CCAGCCTGGG CCCGGCTGGG 
GAGGGGGTGG CAGGGGGCCC TGGTTTTGTG CGGCGCTCCT CCTCCCGCAG CCACAGCCGC 
GTGCGTGCCA TTGCCAGCCG GGCCCGCCAG GCCCAGGAGC GGCAGCAGAG ACTGCAGGGC 
CTGGGCCGGC AGGGACCCCC AGAAGAGGAG CGGGGCACCC CCGAGGGCGC CTGCTCCGTG 
GGCCACGAGG GCAGTGTGGA TGCACCAGCA CCCTCCAAGG GAGCCCTCGG GCCAGCATCC 
GCGGCTGCTG AAAACCTGGT CCTGCTCCGC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( NM_014638.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055453.2 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 1, mRNA.

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CloneID	OHu63097
Clone ID Related Accession (Same CDS sequence)	NM_014638.4 , NM_014638.3
Accession Version	NM_014638.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL139246.21, DQ176850.1, BC043358.1 and BX117873.1. On Nov 23, 2018 this sequence version replaced NM_014638.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: DQ176850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378486.8/ ENSP00000367747.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGGTC CATGGCCCTC CCCCGACAGC CGGACCAAGG GAACGGTGGC CTGGCTGGCG 
GAGGTACTCC TCTGGGTTGG AGGGAGTGTG GTGCTGTCTT CAGAGTGGCA GCTCGGCCCC 
CTGGTGGAGC GGTGCATGGG TGCCATGCAA GAGGGGATGC AGATGGTGAA GCTGCGTGGC 
GGCTCCAAGG GCCTGGTCCG CTTCTACTAC CTGGACGAGC ACCGCTCCTG CATCCGCTGG 
AGGCCCTCAC GCAAGAACGA GAAGGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTGAGT 
GAGGGGCGGC AGTCGGAGGT CTTCCAGCGC TACCCTGACG GCAGCTTCGA CCCCAACTGC 
TGCTTCAGCA TCTACCACGG CAGCCACCGC GAGTCGCTGG ACCTGGTCTC CACCAGCAGC 
GAGGTGGCGC GCACCTGGGT CACTGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG 
GACAGCCTGG CTCGCCGCCA GCGCACCAGG GACCAGTGGC TGAAGCAGAC GTTTGACGAG 
GCCGACAAGA ACGGGGATGG CAGCCTGAGC ATTGGCGAGG TCCTGCAGCT GCTGCACAAG 
CTCAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAGGGAAGC GGACACGGAT 
GACCACCAAG GGACGCTGGG TTTTGAAGAG TTCTGTGCCT TCTACAAGAT GATGTCCACC 
CGCCGGGACC TCTACCTGCT CATGCTGACC TACAGCAACC ACAAGGACCA CCTGGATGCC 
GCCAGCCTGC AGCGCTTCCT GCAGGTGGAG CAGAAGATGG CGGGTGTGAC CCTCGAGAGC 
TGCCAGGACA TCATCGAGCA GTTTGAGCCA TGCCCAGAAA ACAAGAGTAA GGGGCTGCTG 
GGCATTGATG GCTTCACCAA CTACACCAGG AGCCCTGCTG GTGACATCTT CAACCCTGAG 
CACCACCATG TGCACCAGGA CATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCC 
CACAACACCT ACCTCGTGGG TGACCAGCTC ATGTCCCAGT CACGGGTGGA CATGTATGCT 
TGGGTCCTGC AGGCTGGCTG CCGCTGCGTG GAGGTGGACT GCTGGGATGG GCCCGACGGG 
GAGCCCATTG TGCACCATGG CTACACTCTG ACTTCCAAGA TCCTCTTCAA AGACGTCATT 
GAAACCATCA ACAAATATGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATCGAA 
AACCACTGCA GTGTCATCCA GCAGAAGAAA ATGGCCCAGT ATCTGACTGA CATCCTTGGG 
GACAAGCTGG ACCTGTCATC AGTGAGCAGT GAAGATGCCA CCACACTCCC CTCTCCACAG 
ATGCTCAAGG GCAAGATCCT CGTGAAGGGG AAGAAGCTCC CAGCCAACAT CAGCGAGGAT 
GCGGAGGAAG GCGAGGTGTC TGATGAGGAC AGTGCTGATG AGATTGACGA TGACTGCAAG 
CTCCTCAATG GGGATGCATC CACCAATCGA AAGCGTGTAG AAAACACTGC TAAGAGGAAA 
CTGGATTCCC TCATCAAAGA GTCGAAGATT CGGGACTGTG AGGACCCCAA CAACTTCTCC 
GTCTCCACAC TGTCCCCATC TGGAAAGCTC GGACGCAAGA GCAAGGCTGA AGAGGACGTG 
GAGTCTGGGG AGGATGCCGG GGCCAGCAGA CGCAATGGCC GCCTCGTCGT GGGAAGCTTC 
TCCAGGCGCA AGAAGAAGGG CAGCAAGCTG AAGAAGGCGG CCAGCGTGGA GGAGGGAGAT 
GAGGGTCAGG ACTCCCCGGG AGGCCAGAGC CGAGGGGCGA CCCGGCAGAA GAAGACCATG 
AAGCTGTCCC GGGCCCTCTC TGACCTGGTG AAGTACACCA AGTCCGTGGC CACCCACGAC 
ATAGAGATGG AGGCGGCGTC CAGCTGGCAG GTGTCGTCCT TCAGCGAGAC CAAGGCCCAC 
CAGATTCTGC AGCAGAAGCC GGCGCAGTAC CTACGCTTCA ACCAGCAGCA GCTCTCCCGC 
ATCTACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ACCCGCAGCC CTTCTGGAAC 
GCCGGCTGCC AAATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT GCAGCTGAAC 
CGAGCCAAGT TCAGCGCCAA CGGTGGCTGC GGCTACGTAC TCAAGCCTGG GTGCATGTGC 
CAGGGCGTGT TCAACCCCAA CTCGGAGGAC CCCCTGCCCG GGCAGCTCAA GAAGCAGCTG 
GTGCTCCGGA TCATCAGTGG CCAGCAGCTT CCCAAGCCGC GCGACTCCAT GCTGGGGGAC 
CGTGGGGAGA TCATCGACCC CTTTGTGGAG GTGGAGATCA TTGGGCTCCC TGTGGACTGC 
AGCAGGGAGC AGACCCGCGT GGTGGACGAC AACGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGA CACCCGCCCC CTCTCCACGC AGCGGCCACT CCCCCCACTG 
TGCAGCCTGG AAACCATCGC TGAGGAGCCC GCCCCAGGCC CTGGTCCCCC GCCACCAGCG 
GCTGTCCCCA CCAGCTCTTC TCAGGGACGG CCCCCATACC CCACAGGACC CGGAGCCAAT 
GTGGCAAGCC CCCTAGAGGA CACTGAGGAG CCCCGAGACA GCAGGCCTCG GCCGTGCAAC 
GGCGAGGGCG CCGGCGGGGC ATACGAGAGG GCCCCCGGCA GCCAGACGGA CGGCAGGAGC 
CAGCCCCGGA CCCTGGGCCA CCTGCCCGTG ATTAGAAGGG TGAAGAGTGA GGGGCAGGTG 
CCCACGGAGC CCCTGGGAGG GTGGCGGCCC CTGGCCGCTC CCTTTCCAGC TCCTGCCGTG 
TACTCCGATG CCACGGGCAG TGACCCGCTG TGGCAGCGGC TGGAGCCATG TGGCCACCGA 
GACAGCGTTT CCTCCTCCTC CAGCATGTCA TCCAGCGACA CTGTCATTGA CCTCTCCCTG 
CCCAGCCTGG GCCTGGGCCG CAGCCGTGAG AACCTCGCTG GAGCCCACAT GGGACGCCTG 
CCCCCCAGGC CCCACTCGGC TTCGGCTGCC CGCCCAGACC TGCCACCTGT GACCAAGAGC 
AAATCCAACC CCAACCTTCG GGCTACAGGC CAGCGGCCTC CCATACCTGA CGAACTGCAG 
CCCAGGTCCC TGGCCCCAAG GATGGCTGGC CTCCCCTTCC GGCCTCCCTG GGGCTGCCTT 
TCCCTGGTGG GCGTGCAGGA CTGCCCCGTG GCTGCCAAGT CCAAGAGCCT GGGCGACCTC 
ACTGCTGATG ACTTTGCCCC TAGCTTTGAG GGCGGCTCCC GCAGACTGAG CCACAGCCTG 
GGCCTCCCGG GAGGGACACG GCGGGTGTCG GGGCCAGGGG TGAGACGGGA CACCCTGACA 
GAGCAGCTGC GCTGGCTCAC TGTCTTCCAG CAGGCAGGAG ACATCACGTC ACCCACCAGC 
CTGGGCCCGG CTGGGGAGGG GGTGGCAGGG GGCCCTGGTT TTGTGCGGCG CTCCTCCTCC 
CGCAGCCACA GCCGCGTGCG TGCCATTGCC AGCCGGGCCC GCCAGGCCCA GGAGCGGCAG 
CAGAGACTGC AGGGCCTGGG CCGGCAGGGA CCCCCAGAAG AGGAGCGGGG CACCCCCGAG 
GGCGCCTGCT CCGTGGGCCA CGAGGGCAGT GTGGATGCAC CAGCACCCTC CAAGGGAGCC 
CTCGGGCCAG CATCCGCGGC TGCTGAAAAC CTGGTCCTGC TCCGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055453.2
CDS	301..4551
Translation	MSGPWPSPDSRTKGTVAWLAEVLLWVGGSVVLSSEWQLGPLVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRDTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	NM_014638.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014638.4

ORF Insert Sequence:

ATGTCTGGTC CATGGCCCTC CCCCGACAGC CGGACCAAGG GAACGGTGGC CTGGCTGGCG 
GAGGTACTCC TCTGGGTTGG AGGGAGTGTG GTGCTGTCTT CAGAGTGGCA GCTCGGCCCC 
CTGGTGGAGC GGTGCATGGG TGCCATGCAA GAGGGGATGC AGATGGTGAA GCTGCGTGGC 
GGCTCCAAGG GCCTGGTCCG CTTCTACTAC CTGGACGAGC ACCGCTCCTG CATCCGCTGG 
AGGCCCTCAC GCAAGAACGA GAAGGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTGAGT 
GAGGGGCGGC AGTCGGAGGT CTTCCAGCGC TACCCTGACG GCAGCTTCGA CCCCAACTGC 
TGCTTCAGCA TCTACCACGG CAGCCACCGC GAGTCGCTGG ACCTGGTCTC CACCAGCAGC 
GAGGTGGCGC GCACCTGGGT CACTGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG 
GACAGCCTGG CTCGCCGCCA GCGCACCAGG GACCAGTGGC TGAAGCAGAC GTTTGACGAG 
GCCGACAAGA ACGGGGATGG CAGCCTGAGC ATTGGCGAGG TCCTGCAGCT GCTGCACAAG 
CTCAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAGGGAAGC GGACACGGAT 
GACCACCAAG GGACGCTGGG TTTTGAAGAG TTCTGTGCCT TCTACAAGAT GATGTCCACC 
CGCCGGGACC TCTACCTGCT CATGCTGACC TACAGCAACC ACAAGGACCA CCTGGATGCC 
GCCAGCCTGC AGCGCTTCCT GCAGGTGGAG CAGAAGATGG CGGGTGTGAC CCTCGAGAGC 
TGCCAGGACA TCATCGAGCA GTTTGAGCCA TGCCCAGAAA ACAAGAGTAA GGGGCTGCTG 
GGCATTGATG GCTTCACCAA CTACACCAGG AGCCCTGCTG GTGACATCTT CAACCCTGAG 
CACCACCATG TGCACCAGGA CATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCC 
CACAACACCT ACCTCGTGGG TGACCAGCTC ATGTCCCAGT CACGGGTGGA CATGTATGCT 
TGGGTCCTGC AGGCTGGCTG CCGCTGCGTG GAGGTGGACT GCTGGGATGG GCCCGACGGG 
GAGCCCATTG TGCACCATGG CTACACTCTG ACTTCCAAGA TCCTCTTCAA AGACGTCATT 
GAAACCATCA ACAAATATGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATCGAA 
AACCACTGCA GTGTCATCCA GCAGAAGAAA ATGGCCCAGT ATCTGACTGA CATCCTTGGG 
GACAAGCTGG ACCTGTCATC AGTGAGCAGT GAAGATGCCA CCACACTCCC CTCTCCACAG 
ATGCTCAAGG GCAAGATCCT CGTGAAGGGG AAGAAGCTCC CAGCCAACAT CAGCGAGGAT 
GCGGAGGAAG GCGAGGTGTC TGATGAGGAC AGTGCTGATG AGATTGACGA TGACTGCAAG 
CTCCTCAATG GGGATGCATC CACCAATCGA AAGCGTGTAG AAAACACTGC TAAGAGGAAA 
CTGGATTCCC TCATCAAAGA GTCGAAGATT CGGGACTGTG AGGACCCCAA CAACTTCTCC 
GTCTCCACAC TGTCCCCATC TGGAAAGCTC GGACGCAAGA GCAAGGCTGA AGAGGACGTG 
GAGTCTGGGG AGGATGCCGG GGCCAGCAGA CGCAATGGCC GCCTCGTCGT GGGAAGCTTC 
TCCAGGCGCA AGAAGAAGGG CAGCAAGCTG AAGAAGGCGG CCAGCGTGGA GGAGGGAGAT 
GAGGGTCAGG ACTCCCCGGG AGGCCAGAGC CGAGGGGCGA CCCGGCAGAA GAAGACCATG 
AAGCTGTCCC GGGCCCTCTC TGACCTGGTG AAGTACACCA AGTCCGTGGC CACCCACGAC 
ATAGAGATGG AGGCGGCGTC CAGCTGGCAG GTGTCGTCCT TCAGCGAGAC CAAGGCCCAC 
CAGATTCTGC AGCAGAAGCC GGCGCAGTAC CTACGCTTCA ACCAGCAGCA GCTCTCCCGC 
ATCTACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ACCCGCAGCC CTTCTGGAAC 
GCCGGCTGCC AAATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT GCAGCTGAAC 
CGAGCCAAGT TCAGCGCCAA CGGTGGCTGC GGCTACGTAC TCAAGCCTGG GTGCATGTGC 
CAGGGCGTGT TCAACCCCAA CTCGGAGGAC CCCCTGCCCG GGCAGCTCAA GAAGCAGCTG 
GTGCTCCGGA TCATCAGTGG CCAGCAGCTT CCCAAGCCGC GCGACTCCAT GCTGGGGGAC 
CGTGGGGAGA TCATCGACCC CTTTGTGGAG GTGGAGATCA TTGGGCTCCC TGTGGACTGC 
AGCAGGGAGC AGACCCGCGT GGTGGACGAC AACGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGA CACCCGCCCC CTCTCCACGC AGCGGCCACT CCCCCCACTG 
TGCAGCCTGG AAACCATCGC TGAGGAGCCC GCCCCAGGCC CTGGTCCCCC GCCACCAGCG 
GCTGTCCCCA CCAGCTCTTC TCAGGGACGG CCCCCATACC CCACAGGACC CGGAGCCAAT 
GTGGCAAGCC CCCTAGAGGA CACTGAGGAG CCCCGAGACA GCAGGCCTCG GCCGTGCAAC 
GGCGAGGGCG CCGGCGGGGC ATACGAGAGG GCCCCCGGCA GCCAGACGGA CGGCAGGAGC 
CAGCCCCGGA CCCTGGGCCA CCTGCCCGTG ATTAGAAGGG TGAAGAGTGA GGGGCAGGTG 
CCCACGGAGC CCCTGGGAGG GTGGCGGCCC CTGGCCGCTC CCTTTCCAGC TCCTGCCGTG 
TACTCCGATG CCACGGGCAG TGACCCGCTG TGGCAGCGGC TGGAGCCATG TGGCCACCGA 
GACAGCGTTT CCTCCTCCTC CAGCATGTCA TCCAGCGACA CTGTCATTGA CCTCTCCCTG 
CCCAGCCTGG GCCTGGGCCG CAGCCGTGAG AACCTCGCTG GAGCCCACAT GGGACGCCTG 
CCCCCCAGGC CCCACTCGGC TTCGGCTGCC CGCCCAGACC TGCCACCTGT GACCAAGAGC 
AAATCCAACC CCAACCTTCG GGCTACAGGC CAGCGGCCTC CCATACCTGA CGAACTGCAG 
CCCAGGTCCC TGGCCCCAAG GATGGCTGGC CTCCCCTTCC GGCCTCCCTG GGGCTGCCTT 
TCCCTGGTGG GCGTGCAGGA CTGCCCCGTG GCTGCCAAGT CCAAGAGCCT GGGCGACCTC 
ACTGCTGATG ACTTTGCCCC TAGCTTTGAG GGCGGCTCCC GCAGACTGAG CCACAGCCTG 
GGCCTCCCGG GAGGGACACG GCGGGTGTCG GGGCCAGGGG TGAGACGGGA CACCCTGACA 
GAGCAGCTGC GCTGGCTCAC TGTCTTCCAG CAGGCAGGAG ACATCACGTC ACCCACCAGC 
CTGGGCCCGG CTGGGGAGGG GGTGGCAGGG GGCCCTGGTT TTGTGCGGCG CTCCTCCTCC 
CGCAGCCACA GCCGCGTGCG TGCCATTGCC AGCCGGGCCC GCCAGGCCCA GGAGCGGCAG 
CAGAGACTGC AGGGCCTGGG CCGGCAGGGA CCCCCAGAAG AGGAGCGGGG CACCCCCGAG 
GGCGCCTGCT CCGTGGGCCA CGAGGGCAGT GTGGATGCAC CAGCACCCTC CAAGGGAGCC 
CTCGGGCCAG CATCCGCGGC TGCTGAAAAC CTGGTCCTGC TCCGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( NM_014638.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055453.2 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 1, mRNA.

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CloneID	OHu63097
Clone ID Related Accession (Same CDS sequence)	NM_014638.4 , NM_014638.3
Accession Version	NM_014638.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ176850.1, BC043358.1 and BX117873.1. On Dec 12, 2014 this sequence version replaced NM_014638.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: DQ176850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGGTC CATGGCCCTC CCCCGACAGC CGGACCAAGG GAACGGTGGC CTGGCTGGCG 
GAGGTACTCC TCTGGGTTGG AGGGAGTGTG GTGCTGTCTT CAGAGTGGCA GCTCGGCCCC 
CTGGTGGAGC GGTGCATGGG TGCCATGCAA GAGGGGATGC AGATGGTGAA GCTGCGTGGC 
GGCTCCAAGG GCCTGGTCCG CTTCTACTAC CTGGACGAGC ACCGCTCCTG CATCCGCTGG 
AGGCCCTCAC GCAAGAACGA GAAGGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTGAGT 
GAGGGGCGGC AGTCGGAGGT CTTCCAGCGC TACCCTGACG GCAGCTTCGA CCCCAACTGC 
TGCTTCAGCA TCTACCACGG CAGCCACCGC GAGTCGCTGG ACCTGGTCTC CACCAGCAGC 
GAGGTGGCGC GCACCTGGGT CACTGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG 
GACAGCCTGG CTCGCCGCCA GCGCACCAGG GACCAGTGGC TGAAGCAGAC GTTTGACGAG 
GCCGACAAGA ACGGGGATGG CAGCCTGAGC ATTGGCGAGG TCCTGCAGCT GCTGCACAAG 
CTCAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAGGGAAGC GGACACGGAT 
GACCACCAAG GGACGCTGGG TTTTGAAGAG TTCTGTGCCT TCTACAAGAT GATGTCCACC 
CGCCGGGACC TCTACCTGCT CATGCTGACC TACAGCAACC ACAAGGACCA CCTGGATGCC 
GCCAGCCTGC AGCGCTTCCT GCAGGTGGAG CAGAAGATGG CGGGTGTGAC CCTCGAGAGC 
TGCCAGGACA TCATCGAGCA GTTTGAGCCA TGCCCAGAAA ACAAGAGTAA GGGGCTGCTG 
GGCATTGATG GCTTCACCAA CTACACCAGG AGCCCTGCTG GTGACATCTT CAACCCTGAG 
CACCACCATG TGCACCAGGA CATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCC 
CACAACACCT ACCTCGTGGG TGACCAGCTC ATGTCCCAGT CACGGGTGGA CATGTATGCT 
TGGGTCCTGC AGGCTGGCTG CCGCTGCGTG GAGGTGGACT GCTGGGATGG GCCCGACGGG 
GAGCCCATTG TGCACCATGG CTACACTCTG ACTTCCAAGA TCCTCTTCAA AGACGTCATT 
GAAACCATCA ACAAATATGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATCGAA 
AACCACTGCA GTGTCATCCA GCAGAAGAAA ATGGCCCAGT ATCTGACTGA CATCCTTGGG 
GACAAGCTGG ACCTGTCATC AGTGAGCAGT GAAGATGCCA CCACACTCCC CTCTCCACAG 
ATGCTCAAGG GCAAGATCCT CGTGAAGGGG AAGAAGCTCC CAGCCAACAT CAGCGAGGAT 
GCGGAGGAAG GCGAGGTGTC TGATGAGGAC AGTGCTGATG AGATTGACGA TGACTGCAAG 
CTCCTCAATG GGGATGCATC CACCAATCGA AAGCGTGTAG AAAACACTGC TAAGAGGAAA 
CTGGATTCCC TCATCAAAGA GTCGAAGATT CGGGACTGTG AGGACCCCAA CAACTTCTCC 
GTCTCCACAC TGTCCCCATC TGGAAAGCTC GGACGCAAGA GCAAGGCTGA AGAGGACGTG 
GAGTCTGGGG AGGATGCCGG GGCCAGCAGA CGCAATGGCC GCCTCGTCGT GGGAAGCTTC 
TCCAGGCGCA AGAAGAAGGG CAGCAAGCTG AAGAAGGCGG CCAGCGTGGA GGAGGGAGAT 
GAGGGTCAGG ACTCCCCGGG AGGCCAGAGC CGAGGGGCGA CCCGGCAGAA GAAGACCATG 
AAGCTGTCCC GGGCCCTCTC TGACCTGGTG AAGTACACCA AGTCCGTGGC CACCCACGAC 
ATAGAGATGG AGGCGGCGTC CAGCTGGCAG GTGTCGTCCT TCAGCGAGAC CAAGGCCCAC 
CAGATTCTGC AGCAGAAGCC GGCGCAGTAC CTACGCTTCA ACCAGCAGCA GCTCTCCCGC 
ATCTACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ACCCGCAGCC CTTCTGGAAC 
GCCGGCTGCC AAATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT GCAGCTGAAC 
CGAGCCAAGT TCAGCGCCAA CGGTGGCTGC GGCTACGTAC TCAAGCCTGG GTGCATGTGC 
CAGGGCGTGT TCAACCCCAA CTCGGAGGAC CCCCTGCCCG GGCAGCTCAA GAAGCAGCTG 
GTGCTCCGGA TCATCAGTGG CCAGCAGCTT CCCAAGCCGC GCGACTCCAT GCTGGGGGAC 
CGTGGGGAGA TCATCGACCC CTTTGTGGAG GTGGAGATCA TTGGGCTCCC TGTGGACTGC 
AGCAGGGAGC AGACCCGCGT GGTGGACGAC AACGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGA CACCCGCCCC CTCTCCACGC AGCGGCCACT CCCCCCACTG 
TGCAGCCTGG AAACCATCGC TGAGGAGCCC GCCCCAGGCC CTGGTCCCCC GCCACCAGCG 
GCTGTCCCCA CCAGCTCTTC TCAGGGACGG CCCCCATACC CCACAGGACC CGGAGCCAAT 
GTGGCAAGCC CCCTAGAGGA CACTGAGGAG CCCCGAGACA GCAGGCCTCG GCCGTGCAAC 
GGCGAGGGCG CCGGCGGGGC ATACGAGAGG GCCCCCGGCA GCCAGACGGA CGGCAGGAGC 
CAGCCCCGGA CCCTGGGCCA CCTGCCCGTG ATTAGAAGGG TGAAGAGTGA GGGGCAGGTG 
CCCACGGAGC CCCTGGGAGG GTGGCGGCCC CTGGCCGCTC CCTTTCCAGC TCCTGCCGTG 
TACTCCGATG CCACGGGCAG TGACCCGCTG TGGCAGCGGC TGGAGCCATG TGGCCACCGA 
GACAGCGTTT CCTCCTCCTC CAGCATGTCA TCCAGCGACA CTGTCATTGA CCTCTCCCTG 
CCCAGCCTGG GCCTGGGCCG CAGCCGTGAG AACCTCGCTG GAGCCCACAT GGGACGCCTG 
CCCCCCAGGC CCCACTCGGC TTCGGCTGCC CGCCCAGACC TGCCACCTGT GACCAAGAGC 
AAATCCAACC CCAACCTTCG GGCTACAGGC CAGCGGCCTC CCATACCTGA CGAACTGCAG 
CCCAGGTCCC TGGCCCCAAG GATGGCTGGC CTCCCCTTCC GGCCTCCCTG GGGCTGCCTT 
TCCCTGGTGG GCGTGCAGGA CTGCCCCGTG GCTGCCAAGT CCAAGAGCCT GGGCGACCTC 
ACTGCTGATG ACTTTGCCCC TAGCTTTGAG GGCGGCTCCC GCAGACTGAG CCACAGCCTG 
GGCCTCCCGG GAGGGACACG GCGGGTGTCG GGGCCAGGGG TGAGACGGGA CACCCTGACA 
GAGCAGCTGC GCTGGCTCAC TGTCTTCCAG CAGGCAGGAG ACATCACGTC ACCCACCAGC 
CTGGGCCCGG CTGGGGAGGG GGTGGCAGGG GGCCCTGGTT TTGTGCGGCG CTCCTCCTCC 
CGCAGCCACA GCCGCGTGCG TGCCATTGCC AGCCGGGCCC GCCAGGCCCA GGAGCGGCAG 
CAGAGACTGC AGGGCCTGGG CCGGCAGGGA CCCCCAGAAG AGGAGCGGGG CACCCCCGAG 
GGCGCCTGCT CCGTGGGCCA CGAGGGCAGT GTGGATGCAC CAGCACCCTC CAAGGGAGCC 
CTCGGGCCAG CATCCGCGGC TGCTGAAAAC CTGGTCCTGC TCCGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055453.2
CDS	275..4525
Translation	MSGPWPSPDSRTKGTVAWLAEVLLWVGGSVVLSSEWQLGPLVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRDTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	NM_014638.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014638.3

ORF Insert Sequence:

ATGTCTGGTC CATGGCCCTC CCCCGACAGC CGGACCAAGG GAACGGTGGC CTGGCTGGCG 
GAGGTACTCC TCTGGGTTGG AGGGAGTGTG GTGCTGTCTT CAGAGTGGCA GCTCGGCCCC 
CTGGTGGAGC GGTGCATGGG TGCCATGCAA GAGGGGATGC AGATGGTGAA GCTGCGTGGC 
GGCTCCAAGG GCCTGGTCCG CTTCTACTAC CTGGACGAGC ACCGCTCCTG CATCCGCTGG 
AGGCCCTCAC GCAAGAACGA GAAGGCCAAG ATCTCCATCG ACTCCATCCA GGAGGTGAGT 
GAGGGGCGGC AGTCGGAGGT CTTCCAGCGC TACCCTGACG GCAGCTTCGA CCCCAACTGC 
TGCTTCAGCA TCTACCACGG CAGCCACCGC GAGTCGCTGG ACCTGGTCTC CACCAGCAGC 
GAGGTGGCGC GCACCTGGGT CACTGGCCTG CGCTACCTCA TGGCCGGCAT CAGCGACGAG 
GACAGCCTGG CTCGCCGCCA GCGCACCAGG GACCAGTGGC TGAAGCAGAC GTTTGACGAG 
GCCGACAAGA ACGGGGATGG CAGCCTGAGC ATTGGCGAGG TCCTGCAGCT GCTGCACAAG 
CTCAACGTGA ACCTGCCCCG GCAGAGGGTG AAGCAGATGT TCAGGGAAGC GGACACGGAT 
GACCACCAAG GGACGCTGGG TTTTGAAGAG TTCTGTGCCT TCTACAAGAT GATGTCCACC 
CGCCGGGACC TCTACCTGCT CATGCTGACC TACAGCAACC ACAAGGACCA CCTGGATGCC 
GCCAGCCTGC AGCGCTTCCT GCAGGTGGAG CAGAAGATGG CGGGTGTGAC CCTCGAGAGC 
TGCCAGGACA TCATCGAGCA GTTTGAGCCA TGCCCAGAAA ACAAGAGTAA GGGGCTGCTG 
GGCATTGATG GCTTCACCAA CTACACCAGG AGCCCTGCTG GTGACATCTT CAACCCTGAG 
CACCACCATG TGCACCAGGA CATGACGCAG CCGCTGAGCC ACTACTTCAT CACCTCGTCC 
CACAACACCT ACCTCGTGGG TGACCAGCTC ATGTCCCAGT CACGGGTGGA CATGTATGCT 
TGGGTCCTGC AGGCTGGCTG CCGCTGCGTG GAGGTGGACT GCTGGGATGG GCCCGACGGG 
GAGCCCATTG TGCACCATGG CTACACTCTG ACTTCCAAGA TCCTCTTCAA AGACGTCATT 
GAAACCATCA ACAAATATGC CTTCATCAAG AATGAGTACC CAGTGATCCT GTCCATCGAA 
AACCACTGCA GTGTCATCCA GCAGAAGAAA ATGGCCCAGT ATCTGACTGA CATCCTTGGG 
GACAAGCTGG ACCTGTCATC AGTGAGCAGT GAAGATGCCA CCACACTCCC CTCTCCACAG 
ATGCTCAAGG GCAAGATCCT CGTGAAGGGG AAGAAGCTCC CAGCCAACAT CAGCGAGGAT 
GCGGAGGAAG GCGAGGTGTC TGATGAGGAC AGTGCTGATG AGATTGACGA TGACTGCAAG 
CTCCTCAATG GGGATGCATC CACCAATCGA AAGCGTGTAG AAAACACTGC TAAGAGGAAA 
CTGGATTCCC TCATCAAAGA GTCGAAGATT CGGGACTGTG AGGACCCCAA CAACTTCTCC 
GTCTCCACAC TGTCCCCATC TGGAAAGCTC GGACGCAAGA GCAAGGCTGA AGAGGACGTG 
GAGTCTGGGG AGGATGCCGG GGCCAGCAGA CGCAATGGCC GCCTCGTCGT GGGAAGCTTC 
TCCAGGCGCA AGAAGAAGGG CAGCAAGCTG AAGAAGGCGG CCAGCGTGGA GGAGGGAGAT 
GAGGGTCAGG ACTCCCCGGG AGGCCAGAGC CGAGGGGCGA CCCGGCAGAA GAAGACCATG 
AAGCTGTCCC GGGCCCTCTC TGACCTGGTG AAGTACACCA AGTCCGTGGC CACCCACGAC 
ATAGAGATGG AGGCGGCGTC CAGCTGGCAG GTGTCGTCCT TCAGCGAGAC CAAGGCCCAC 
CAGATTCTGC AGCAGAAGCC GGCGCAGTAC CTACGCTTCA ACCAGCAGCA GCTCTCCCGC 
ATCTACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ACCCGCAGCC CTTCTGGAAC 
GCCGGCTGCC AAATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT GCAGCTGAAC 
CGAGCCAAGT TCAGCGCCAA CGGTGGCTGC GGCTACGTAC TCAAGCCTGG GTGCATGTGC 
CAGGGCGTGT TCAACCCCAA CTCGGAGGAC CCCCTGCCCG GGCAGCTCAA GAAGCAGCTG 
GTGCTCCGGA TCATCAGTGG CCAGCAGCTT CCCAAGCCGC GCGACTCCAT GCTGGGGGAC 
CGTGGGGAGA TCATCGACCC CTTTGTGGAG GTGGAGATCA TTGGGCTCCC TGTGGACTGC 
AGCAGGGAGC AGACCCGCGT GGTGGACGAC AACGGGTTCA ACCCCACCTG GGAGGAGACC 
CTGGTTTTCA TGGTGCACAT GCCGGAGATC GCGCTGGTCC GCTTCCTCGT CTGGGACCAC 
GATCCCATCG GGCGTGACTT CATTGGCCAG AGGACGCTGG CCTTCAGCAG CATGATGCCA 
GGCTACAGAC ACGTGTACCT AGAAGGGATG GAAGAGGCCT CCATCTTCGT GCATGTGGCT 
GTCAGTGACA TCAGCGGTAA GGTCAAGCAG GCTCTGGGCC TAAAAGGCCT CTTCCTCCGA 
GGCCCAAAGC CCGGCTCGCT GGACAGTCAT GCTGCTGGGC GGCCCCCGGC CCGGCCCTCC 
GTTAGCCAGC GGATCCTGCG GCGCACGGCC AGCGCCCCGA CCAAGAGCCA GAAGCCGGGC 
CGCAGGGGCT TCCCGGAGCT GGTCCTGGGT ACACGGGACA CAGGCTCCAA GGGGGTGGCA 
GACGATGTGG TGCCCCCCGG GCCCGGACCT GCTCCGGAAG CCCCAGCCCA GGAGGGGCCC 
GGCAGCGGCA GCCCCCGAGA CACCCGCCCC CTCTCCACGC AGCGGCCACT CCCCCCACTG 
TGCAGCCTGG AAACCATCGC TGAGGAGCCC GCCCCAGGCC CTGGTCCCCC GCCACCAGCG 
GCTGTCCCCA CCAGCTCTTC TCAGGGACGG CCCCCATACC CCACAGGACC CGGAGCCAAT 
GTGGCAAGCC CCCTAGAGGA CACTGAGGAG CCCCGAGACA GCAGGCCTCG GCCGTGCAAC 
GGCGAGGGCG CCGGCGGGGC ATACGAGAGG GCCCCCGGCA GCCAGACGGA CGGCAGGAGC 
CAGCCCCGGA CCCTGGGCCA CCTGCCCGTG ATTAGAAGGG TGAAGAGTGA GGGGCAGGTG 
CCCACGGAGC CCCTGGGAGG GTGGCGGCCC CTGGCCGCTC CCTTTCCAGC TCCTGCCGTG 
TACTCCGATG CCACGGGCAG TGACCCGCTG TGGCAGCGGC TGGAGCCATG TGGCCACCGA 
GACAGCGTTT CCTCCTCCTC CAGCATGTCA TCCAGCGACA CTGTCATTGA CCTCTCCCTG 
CCCAGCCTGG GCCTGGGCCG CAGCCGTGAG AACCTCGCTG GAGCCCACAT GGGACGCCTG 
CCCCCCAGGC CCCACTCGGC TTCGGCTGCC CGCCCAGACC TGCCACCTGT GACCAAGAGC 
AAATCCAACC CCAACCTTCG GGCTACAGGC CAGCGGCCTC CCATACCTGA CGAACTGCAG 
CCCAGGTCCC TGGCCCCAAG GATGGCTGGC CTCCCCTTCC GGCCTCCCTG GGGCTGCCTT 
TCCCTGGTGG GCGTGCAGGA CTGCCCCGTG GCTGCCAAGT CCAAGAGCCT GGGCGACCTC 
ACTGCTGATG ACTTTGCCCC TAGCTTTGAG GGCGGCTCCC GCAGACTGAG CCACAGCCTG 
GGCCTCCCGG GAGGGACACG GCGGGTGTCG GGGCCAGGGG TGAGACGGGA CACCCTGACA 
GAGCAGCTGC GCTGGCTCAC TGTCTTCCAG CAGGCAGGAG ACATCACGTC ACCCACCAGC 
CTGGGCCCGG CTGGGGAGGG GGTGGCAGGG GGCCCTGGTT TTGTGCGGCG CTCCTCCTCC 
CGCAGCCACA GCCGCGTGCG TGCCATTGCC AGCCGGGCCC GCCAGGCCCA GGAGCGGCAG 
CAGAGACTGC AGGGCCTGGG CCGGCAGGGA CCCCCAGAAG AGGAGCGGGG CACCCCCGAG 
GGCGCCTGCT CCGTGGGCCA CGAGGGCAGT GTGGATGCAC CAGCACCCTC CAAGGGAGCC 
CTCGGGCCAG CATCCGCGGC TGCTGAAAAC CTGGTCCTGC TCCGCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_011542455.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540757.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu63087
Clone ID Related Accession (Same CDS sequence)	XM_011542455.2 , XM_011542456.2
Accession Version	XM_011542455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGGTTC AACCCCACCT GGGAGGAGAC CCTGGTTTTC 
ATGGTGCACA TGCCGGAGAT CGCGCTGGTC CGCTTCCTCG TCTGGGACCA CGATCCCATC 
GGGCGTGACT TCATTGGCCA GAGGACGCTG GCCTTCAGCA GCATGATGCC AGGCTACAGA 
CACGTGTACC TAGAAGGGAT GGAAGAGGCC TCCATCTTCG TGCATGTGGC TGTCAGTGAC 
ATCAGCGGTA AGGTCAAGCA GGCTCTGGGC CTAAAAGGCC TCTTCCTCCG AGGCCCAAAG 
CCCGGCTCGC TGGACAGTCA TGCTGCTGGG CGGCCCCCGG CCCGGCCCTC CGTTAGCCAG 
CGGATCCTGC GGCGCACGGC CAGCGCCCCG ACCAAGAGCC AGAAGCCGGG CCGCAGGGGC 
TTCCCGGAGC TGGTCCTGGG TACACGGGAC ACAGGCTCCA AGGGGGTGGC AGACGATGTG 
GTGCCCCCCG GGCCCGGACC TGCTCCGGAA GCCCCAGCCC AGGAGGGGCC CGGCAGCGGC 
AGCCCCCGAG GTAAGGCGCC AGCTGCGGTG GCAGAGAAGA GCCCTGTGCG AGTGCGGCCC 
CCGCGTGTCC TGGACGGCCC CGGGCCTGCT GGGATGGCCG CCACATGCAT GAAGTGTGTG 
GTGGGATCCT GCGCCGGCGT GAACACCGGG GGCCTGCAGA GGGAGCGGCC ACCCAGCCCG 
GGGCCTGCAA GCAGGCAGGC AGCCATTCGC CAGCAGCCCC GGGCCCGGGC TGACTCACTG 
GGGGCCCCCT GCTGTGGCCT GGACCCTCAC GCTATCCCGG GGAGAAGCAG AGAGGCCCCC 
AAGGGTCCTG GGGCCTGGAG GCAGGGTCCA GGCGGTAGCG GCTCCATGTC CTCGGACTCC 
AGCAGCCCAG ACAGCCCGGG CATCCCCGAA AGGTCCCCCC GCTGGCCTGA GGGTGCCTGC 
AGGCAACCGG GGGCCCTGCA GGGAGAGATG AGTGCCTTGT TTGCTCAAAA GCTGGAGGAG 
ATCAGGAGTA AATCCCCCAT GTTCTCCGCC GACACCCGCC CCCTCTCCAC GCAGCGGCCA 
CTCCCCCCAC TGTGCAGCCT GGAAACCATC GCTGAGGAGC CCGCCCCAGG CCCTGGTCCC 
CCGCCACCAG CGGCTGTCCC CACCAGCTCT TCTCAGGGAC GGCCCCCATA CCCCACAGGA 
CCCGGAGCCA ATGTGGCAAG CCCCCTAGAG GACACTGAGG AGCCCCGAGA CAGCAGGCCT 
CGGCCGTGCA ACGGCGAGGG CGCCGGCGGG GCATACGAGA GGGCCCCCGG CAGCCAGACG 
GACGGCAGGA GCCAGCCCCG GACCCTGGGC CACCTGCCCG TGATTAGAAG GGTGAAGAGT 
GAGGGGCAGG TGCCCACGGA GCCCCTGGGA GGGTGGCGGC CCCTGGCCGC TCCCTTTCCA 
GCTCCTGCCG TGTACTCCGA TGCCACGGGC AGTGACCCGC TGTGGCAGCG GCTGGAGCCA 
TGTGGCCACC GAGACAGCGT TTCCTCCTCC TCCAGCATGT CATCCAGCGA CACTGTCATT 
GACCTCTCCC TGCCCAGCCT GGGCCTGGGC CGCAGCCGTG AGAACCTCGC TGGAGCCCAC 
ATGGGACGCC TGCCCCCCAG GCCCCACTCG GCTTCGGCTG CCCGCCCAGA CCTGCCACCT 
GTGACCAAGA GCAAATCCAA CCCCAACCTT CGGGCTACAG GCCAGCGGCC TCCCATACCT 
GACGAACTGC AGCCCAGGTC CCTGGCCCCA AGGATGGCTG GCCTCCCCTT CCGGCCTCCC 
TGGGGCTGCC TTTCCCTGGT GGGCGTGCAG GACTGCCCCG TGGCTGCCAA GTCCAAGAGC 
CTGGGCGACC TCACTGCTGA TGACTTTGCC CCTAGCTTTG AGGGCGGCTC CCGCAGACTG 
AGCCACAGCC TGGGCCTCCC GGGAGGGACA CGGCGGGTGT CGGGGCCAGG GGTGAGACGG 
GACACCCTGA CAGAGCAGCT GCGCTGGCTC ACTGTCTTCC AGCAGGCAGG AGACATCACG 
TCACCCACCA GCCTGGGCCC GGCTGGGGAG GGGGTGGCAG GGGGCCCTGG TTTTGTGCGG 
CGCTCCTCCT CCCGCAGCCA CAGCCGCGTG CGTGCCATTG CCAGCCGGGC CCGCCAGGCC 
CAGGAGCGGC AGCAGAGACT GCAGGGCCTG GGCCGGCAGG GACCCCCAGA AGAGGAGCGG 
GGCACCCCCG AGGGCGCCTG CTCCGTGGGC CACGAGGGCA GTGTGGATGC ACCAGCACCC 
TCCAAGGGAG CCCTCGGGCC AGCATCCGCG GCTGCTGAAA ACCTGGTCCT GCTCCGCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540757.1
CDS	326..5068
Translation	MMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_011542455.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542455.2

ORF Insert Sequence:

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGGTTC AACCCCACCT GGGAGGAGAC CCTGGTTTTC 
ATGGTGCACA TGCCGGAGAT CGCGCTGGTC CGCTTCCTCG TCTGGGACCA CGATCCCATC 
GGGCGTGACT TCATTGGCCA GAGGACGCTG GCCTTCAGCA GCATGATGCC AGGCTACAGA 
CACGTGTACC TAGAAGGGAT GGAAGAGGCC TCCATCTTCG TGCATGTGGC TGTCAGTGAC 
ATCAGCGGTA AGGTCAAGCA GGCTCTGGGC CTAAAAGGCC TCTTCCTCCG AGGCCCAAAG 
CCCGGCTCGC TGGACAGTCA TGCTGCTGGG CGGCCCCCGG CCCGGCCCTC CGTTAGCCAG 
CGGATCCTGC GGCGCACGGC CAGCGCCCCG ACCAAGAGCC AGAAGCCGGG CCGCAGGGGC 
TTCCCGGAGC TGGTCCTGGG TACACGGGAC ACAGGCTCCA AGGGGGTGGC AGACGATGTG 
GTGCCCCCCG GGCCCGGACC TGCTCCGGAA GCCCCAGCCC AGGAGGGGCC CGGCAGCGGC 
AGCCCCCGAG GTAAGGCGCC AGCTGCGGTG GCAGAGAAGA GCCCTGTGCG AGTGCGGCCC 
CCGCGTGTCC TGGACGGCCC CGGGCCTGCT GGGATGGCCG CCACATGCAT GAAGTGTGTG 
GTGGGATCCT GCGCCGGCGT GAACACCGGG GGCCTGCAGA GGGAGCGGCC ACCCAGCCCG 
GGGCCTGCAA GCAGGCAGGC AGCCATTCGC CAGCAGCCCC GGGCCCGGGC TGACTCACTG 
GGGGCCCCCT GCTGTGGCCT GGACCCTCAC GCTATCCCGG GGAGAAGCAG AGAGGCCCCC 
AAGGGTCCTG GGGCCTGGAG GCAGGGTCCA GGCGGTAGCG GCTCCATGTC CTCGGACTCC 
AGCAGCCCAG ACAGCCCGGG CATCCCCGAA AGGTCCCCCC GCTGGCCTGA GGGTGCCTGC 
AGGCAACCGG GGGCCCTGCA GGGAGAGATG AGTGCCTTGT TTGCTCAAAA GCTGGAGGAG 
ATCAGGAGTA AATCCCCCAT GTTCTCCGCC GACACCCGCC CCCTCTCCAC GCAGCGGCCA 
CTCCCCCCAC TGTGCAGCCT GGAAACCATC GCTGAGGAGC CCGCCCCAGG CCCTGGTCCC 
CCGCCACCAG CGGCTGTCCC CACCAGCTCT TCTCAGGGAC GGCCCCCATA CCCCACAGGA 
CCCGGAGCCA ATGTGGCAAG CCCCCTAGAG GACACTGAGG AGCCCCGAGA CAGCAGGCCT 
CGGCCGTGCA ACGGCGAGGG CGCCGGCGGG GCATACGAGA GGGCCCCCGG CAGCCAGACG 
GACGGCAGGA GCCAGCCCCG GACCCTGGGC CACCTGCCCG TGATTAGAAG GGTGAAGAGT 
GAGGGGCAGG TGCCCACGGA GCCCCTGGGA GGGTGGCGGC CCCTGGCCGC TCCCTTTCCA 
GCTCCTGCCG TGTACTCCGA TGCCACGGGC AGTGACCCGC TGTGGCAGCG GCTGGAGCCA 
TGTGGCCACC GAGACAGCGT TTCCTCCTCC TCCAGCATGT CATCCAGCGA CACTGTCATT 
GACCTCTCCC TGCCCAGCCT GGGCCTGGGC CGCAGCCGTG AGAACCTCGC TGGAGCCCAC 
ATGGGACGCC TGCCCCCCAG GCCCCACTCG GCTTCGGCTG CCCGCCCAGA CCTGCCACCT 
GTGACCAAGA GCAAATCCAA CCCCAACCTT CGGGCTACAG GCCAGCGGCC TCCCATACCT 
GACGAACTGC AGCCCAGGTC CCTGGCCCCA AGGATGGCTG GCCTCCCCTT CCGGCCTCCC 
TGGGGCTGCC TTTCCCTGGT GGGCGTGCAG GACTGCCCCG TGGCTGCCAA GTCCAAGAGC 
CTGGGCGACC TCACTGCTGA TGACTTTGCC CCTAGCTTTG AGGGCGGCTC CCGCAGACTG 
AGCCACAGCC TGGGCCTCCC GGGAGGGACA CGGCGGGTGT CGGGGCCAGG GGTGAGACGG 
GACACCCTGA CAGAGCAGCT GCGCTGGCTC ACTGTCTTCC AGCAGGCAGG AGACATCACG 
TCACCCACCA GCCTGGGCCC GGCTGGGGAG GGGGTGGCAG GGGGCCCTGG TTTTGTGCGG 
CGCTCCTCCT CCCGCAGCCA CAGCCGCGTG CGTGCCATTG CCAGCCGGGC CCGCCAGGCC 
CAGGAGCGGC AGCAGAGACT GCAGGGCCTG GGCCGGCAGG GACCCCCAGA AGAGGAGCGG 
GGCACCCCCG AGGGCGCCTG CTCCGTGGGC CACGAGGGCA GTGTGGATGC ACCAGCACCC 
TCCAAGGGAG CCCTCGGGCC AGCATCCGCG GCTGCTGAAA ACCTGGTCCT GCTCCGCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH2 ( XM_011542456.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540758.1 Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

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CloneID	OHu63087
Clone ID Related Accession (Same CDS sequence)	XM_011542455.2 , XM_011542456.2
Accession Version	XM_011542456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGGTTC AACCCCACCT GGGAGGAGAC CCTGGTTTTC 
ATGGTGCACA TGCCGGAGAT CGCGCTGGTC CGCTTCCTCG TCTGGGACCA CGATCCCATC 
GGGCGTGACT TCATTGGCCA GAGGACGCTG GCCTTCAGCA GCATGATGCC AGGCTACAGA 
CACGTGTACC TAGAAGGGAT GGAAGAGGCC TCCATCTTCG TGCATGTGGC TGTCAGTGAC 
ATCAGCGGTA AGGTCAAGCA GGCTCTGGGC CTAAAAGGCC TCTTCCTCCG AGGCCCAAAG 
CCCGGCTCGC TGGACAGTCA TGCTGCTGGG CGGCCCCCGG CCCGGCCCTC CGTTAGCCAG 
CGGATCCTGC GGCGCACGGC CAGCGCCCCG ACCAAGAGCC AGAAGCCGGG CCGCAGGGGC 
TTCCCGGAGC TGGTCCTGGG TACACGGGAC ACAGGCTCCA AGGGGGTGGC AGACGATGTG 
GTGCCCCCCG GGCCCGGACC TGCTCCGGAA GCCCCAGCCC AGGAGGGGCC CGGCAGCGGC 
AGCCCCCGAG GTAAGGCGCC AGCTGCGGTG GCAGAGAAGA GCCCTGTGCG AGTGCGGCCC 
CCGCGTGTCC TGGACGGCCC CGGGCCTGCT GGGATGGCCG CCACATGCAT GAAGTGTGTG 
GTGGGATCCT GCGCCGGCGT GAACACCGGG GGCCTGCAGA GGGAGCGGCC ACCCAGCCCG 
GGGCCTGCAA GCAGGCAGGC AGCCATTCGC CAGCAGCCCC GGGCCCGGGC TGACTCACTG 
GGGGCCCCCT GCTGTGGCCT GGACCCTCAC GCTATCCCGG GGAGAAGCAG AGAGGCCCCC 
AAGGGTCCTG GGGCCTGGAG GCAGGGTCCA GGCGGTAGCG GCTCCATGTC CTCGGACTCC 
AGCAGCCCAG ACAGCCCGGG CATCCCCGAA AGGTCCCCCC GCTGGCCTGA GGGTGCCTGC 
AGGCAACCGG GGGCCCTGCA GGGAGAGATG AGTGCCTTGT TTGCTCAAAA GCTGGAGGAG 
ATCAGGAGTA AATCCCCCAT GTTCTCCGCC GACACCCGCC CCCTCTCCAC GCAGCGGCCA 
CTCCCCCCAC TGTGCAGCCT GGAAACCATC GCTGAGGAGC CCGCCCCAGG CCCTGGTCCC 
CCGCCACCAG CGGCTGTCCC CACCAGCTCT TCTCAGGGAC GGCCCCCATA CCCCACAGGA 
CCCGGAGCCA ATGTGGCAAG CCCCCTAGAG GACACTGAGG AGCCCCGAGA CAGCAGGCCT 
CGGCCGTGCA ACGGCGAGGG CGCCGGCGGG GCATACGAGA GGGCCCCCGG CAGCCAGACG 
GACGGCAGGA GCCAGCCCCG GACCCTGGGC CACCTGCCCG TGATTAGAAG GGTGAAGAGT 
GAGGGGCAGG TGCCCACGGA GCCCCTGGGA GGGTGGCGGC CCCTGGCCGC TCCCTTTCCA 
GCTCCTGCCG TGTACTCCGA TGCCACGGGC AGTGACCCGC TGTGGCAGCG GCTGGAGCCA 
TGTGGCCACC GAGACAGCGT TTCCTCCTCC TCCAGCATGT CATCCAGCGA CACTGTCATT 
GACCTCTCCC TGCCCAGCCT GGGCCTGGGC CGCAGCCGTG AGAACCTCGC TGGAGCCCAC 
ATGGGACGCC TGCCCCCCAG GCCCCACTCG GCTTCGGCTG CCCGCCCAGA CCTGCCACCT 
GTGACCAAGA GCAAATCCAA CCCCAACCTT CGGGCTACAG GCCAGCGGCC TCCCATACCT 
GACGAACTGC AGCCCAGGTC CCTGGCCCCA AGGATGGCTG GCCTCCCCTT CCGGCCTCCC 
TGGGGCTGCC TTTCCCTGGT GGGCGTGCAG GACTGCCCCG TGGCTGCCAA GTCCAAGAGC 
CTGGGCGACC TCACTGCTGA TGACTTTGCC CCTAGCTTTG AGGGCGGCTC CCGCAGACTG 
AGCCACAGCC TGGGCCTCCC GGGAGGGACA CGGCGGGTGT CGGGGCCAGG GGTGAGACGG 
GACACCCTGA CAGAGCAGCT GCGCTGGCTC ACTGTCTTCC AGCAGGCAGG AGACATCACG 
TCACCCACCA GCCTGGGCCC GGCTGGGGAG GGGGTGGCAG GGGGCCCTGG TTTTGTGCGG 
CGCTCCTCCT CCCGCAGCCA CAGCCGCGTG CGTGCCATTG CCAGCCGGGC CCGCCAGGCC 
CAGGAGCGGC AGCAGAGACT GCAGGGCCTG GGCCGGCAGG GACCCCCAGA AGAGGAGCGG 
GGCACCCCCG AGGGCGCCTG CTCCGTGGGC CACGAGGGCA GTGTGGATGC ACCAGCACCC 
TCCAAGGGAG CCCTCGGGCC AGCATCCGCG GCTGCTGAAA ACCTGGTCCT GCTCCGCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540758.1
CDS	283..5025
Translation	MMAPPTAGPLPGPALPPEDPGPDPESRWLFLSANILPVVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRGKAPAAVAEKSPVRVRPPRVLDGPGPAGMAATCMKCVVGSCAGVNTGGLQRERPPSPGPASRQAAIRQQPRARADSLGAPCCGLDPHAIPGRSREAPKGPGAWRQGPGGSGSMSSDSSSPDSPGIPERSPRWPEGACRQPGALQGEMSALFAQKLEEIRSKSPMFSADTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL

Target ORF information:

RefSeq Version	XM_011542456.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 2 (PLCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542456.2

ORF Insert Sequence:

ATGATGGCTC CCCCGACAGC CGGCCCCCTT CCTGGCCCAG CTCTTCCGCC TGAGGACCCA 
GGGCCGGATC CGGAGAGCAG GTGGCTTTTC TTGAGCGCCA ACATTCTGCC CGTGGTGGAG 
CGGTGCATGG GTGCCATGCA AGAGGGGATG CAGATGGTGA AGCTGCGTGG CGGCTCCAAG 
GGCCTGGTCC GCTTCTACTA CCTGGACGAG CACCGCTCCT GCATCCGCTG GAGGCCCTCA 
CGCAAGAACG AGAAGGCCAA GATCTCCATC GACTCCATCC AGGAGGTGAG TGAGGGGCGG 
CAGTCGGAGG TCTTCCAGCG CTACCCTGAC GGCAGCTTCG ACCCCAACTG CTGCTTCAGC 
ATCTACCACG GCAGCCACCG CGAGTCGCTG GACCTGGTCT CCACCAGCAG CGAGGTGGCG 
CGCACCTGGG TCACTGGCCT GCGCTACCTC ATGGCCGGCA TCAGCGACGA GGACAGCCTG 
GCTCGCCGCC AGCGCACCAG GGACCAGTGG CTGAAGCAGA CGTTTGACGA GGCCGACAAG 
AACGGGGATG GCAGCCTGAG CATTGGCGAG GTCCTGCAGC TGCTGCACAA GCTCAACGTG 
AACCTGCCCC GGCAGAGGGT GAAGCAGATG TTCAGGGAAG CGGACACGGA TGACCACCAA 
GGGACGCTGG GTTTTGAAGA GTTCTGTGCC TTCTACAAGA TGATGTCCAC CCGCCGGGAC 
CTCTACCTGC TCATGCTGAC CTACAGCAAC CACAAGGACC ACCTGGATGC CGCCAGCCTG 
CAGCGCTTCC TGCAGGTGGA GCAGAAGATG GCGGGTGTGA CCCTCGAGAG CTGCCAGGAC 
ATCATCGAGC AGTTTGAGCC ATGCCCAGAA AACAAGAGTA AGGGGCTGCT GGGCATTGAT 
GGCTTCACCA ACTACACCAG GAGCCCTGCT GGTGACATCT TCAACCCTGA GCACCACCAT 
GTGCACCAGG ACATGACGCA GCCGCTGAGC CACTACTTCA TCACCTCGTC CCACAACACC 
TACCTCGTGG GTGACCAGCT CATGTCCCAG TCACGGGTGG ACATGTATGC TTGGGTCCTG 
CAGGCTGGCT GCCGCTGCGT GGAGGTGGAC TGCTGGGATG GGCCCGACGG GGAGCCCATT 
GTGCACCATG GCTACACTCT GACTTCCAAG ATCCTCTTCA AAGACGTCAT TGAAACCATC 
AACAAATATG CCTTCATCAA GAATGAGTAC CCAGTGATCC TGTCCATCGA AAACCACTGC 
AGTGTCATCC AGCAGAAGAA AATGGCCCAG TATCTGACTG ACATCCTTGG GGACAAGCTG 
GACCTGTCAT CAGTGAGCAG TGAAGATGCC ACCACACTCC CCTCTCCACA GATGCTCAAG 
GGCAAGATCC TCGTGAAGGG GAAGAAGCTC CCAGCCAACA TCAGCGAGGA TGCGGAGGAA 
GGCGAGGTGT CTGATGAGGA CAGTGCTGAT GAGATTGACG ATGACTGCAA GCTCCTCAAT 
GGGGATGCAT CCACCAATCG AAAGCGTGTA GAAAACACTG CTAAGAGGAA ACTGGATTCC 
CTCATCAAAG AGTCGAAGAT TCGGGACTGT GAGGACCCCA ACAACTTCTC CGTCTCCACA 
CTGTCCCCAT CTGGAAAGCT CGGACGCAAG AGCAAGGCTG AAGAGGACGT GGAGTCTGGG 
GAGGATGCCG GGGCCAGCAG ACGCAATGGC CGCCTCGTCG TGGGAAGCTT CTCCAGGCGC 
AAGAAGAAGG GCAGCAAGCT GAAGAAGGCG GCCAGCGTGG AGGAGGGAGA TGAGGGTCAG 
GACTCCCCGG GAGGCCAGAG CCGAGGGGCG ACCCGGCAGA AGAAGACCAT GAAGCTGTCC 
CGGGCCCTCT CTGACCTGGT GAAGTACACC AAGTCCGTGG CCACCCACGA CATAGAGATG 
GAGGCGGCGT CCAGCTGGCA GGTGTCGTCC TTCAGCGAGA CCAAGGCCCA CCAGATTCTG 
CAGCAGAAGC CGGCGCAGTA CCTACGCTTC AACCAGCAGC AGCTCTCCCG CATCTACCCC 
TCCTCCTACC GTGTGGACTC CAGCAACTAC AACCCGCAGC CCTTCTGGAA CGCCGGCTGC 
CAAATGGTTG CCCTGAACTA CCAGTCAGAG GGGCGGATGC TGCAGCTGAA CCGAGCCAAG 
TTCAGCGCCA ACGGTGGCTG CGGCTACGTA CTCAAGCCTG GGTGCATGTG CCAGGGCGTG 
TTCAACCCCA ACTCGGAGGA CCCCCTGCCC GGGCAGCTCA AGAAGCAGCT GGTGCTCCGG 
ATCATCAGTG GCCAGCAGCT TCCCAAGCCG CGCGACTCCA TGCTGGGGGA CCGTGGGGAG 
ATCATCGACC CCTTTGTGGA GGTGGAGATC ATTGGGCTCC CTGTGGACTG CAGCAGGGAG 
CAGACCCGCG TGGTGGACGA CAACGGGTTC AACCCCACCT GGGAGGAGAC CCTGGTTTTC 
ATGGTGCACA TGCCGGAGAT CGCGCTGGTC CGCTTCCTCG TCTGGGACCA CGATCCCATC 
GGGCGTGACT TCATTGGCCA GAGGACGCTG GCCTTCAGCA GCATGATGCC AGGCTACAGA 
CACGTGTACC TAGAAGGGAT GGAAGAGGCC TCCATCTTCG TGCATGTGGC TGTCAGTGAC 
ATCAGCGGTA AGGTCAAGCA GGCTCTGGGC CTAAAAGGCC TCTTCCTCCG AGGCCCAAAG 
CCCGGCTCGC TGGACAGTCA TGCTGCTGGG CGGCCCCCGG CCCGGCCCTC CGTTAGCCAG 
CGGATCCTGC GGCGCACGGC CAGCGCCCCG ACCAAGAGCC AGAAGCCGGG CCGCAGGGGC 
TTCCCGGAGC TGGTCCTGGG TACACGGGAC ACAGGCTCCA AGGGGGTGGC AGACGATGTG 
GTGCCCCCCG GGCCCGGACC TGCTCCGGAA GCCCCAGCCC AGGAGGGGCC CGGCAGCGGC 
AGCCCCCGAG GTAAGGCGCC AGCTGCGGTG GCAGAGAAGA GCCCTGTGCG AGTGCGGCCC 
CCGCGTGTCC TGGACGGCCC CGGGCCTGCT GGGATGGCCG CCACATGCAT GAAGTGTGTG 
GTGGGATCCT GCGCCGGCGT GAACACCGGG GGCCTGCAGA GGGAGCGGCC ACCCAGCCCG 
GGGCCTGCAA GCAGGCAGGC AGCCATTCGC CAGCAGCCCC GGGCCCGGGC TGACTCACTG 
GGGGCCCCCT GCTGTGGCCT GGACCCTCAC GCTATCCCGG GGAGAAGCAG AGAGGCCCCC 
AAGGGTCCTG GGGCCTGGAG GCAGGGTCCA GGCGGTAGCG GCTCCATGTC CTCGGACTCC 
AGCAGCCCAG ACAGCCCGGG CATCCCCGAA AGGTCCCCCC GCTGGCCTGA GGGTGCCTGC 
AGGCAACCGG GGGCCCTGCA GGGAGAGATG AGTGCCTTGT TTGCTCAAAA GCTGGAGGAG 
ATCAGGAGTA AATCCCCCAT GTTCTCCGCC GACACCCGCC CCCTCTCCAC GCAGCGGCCA 
CTCCCCCCAC TGTGCAGCCT GGAAACCATC GCTGAGGAGC CCGCCCCAGG CCCTGGTCCC 
CCGCCACCAG CGGCTGTCCC CACCAGCTCT TCTCAGGGAC GGCCCCCATA CCCCACAGGA 
CCCGGAGCCA ATGTGGCAAG CCCCCTAGAG GACACTGAGG AGCCCCGAGA CAGCAGGCCT 
CGGCCGTGCA ACGGCGAGGG CGCCGGCGGG GCATACGAGA GGGCCCCCGG CAGCCAGACG 
GACGGCAGGA GCCAGCCCCG GACCCTGGGC CACCTGCCCG TGATTAGAAG GGTGAAGAGT 
GAGGGGCAGG TGCCCACGGA GCCCCTGGGA GGGTGGCGGC CCCTGGCCGC TCCCTTTCCA 
GCTCCTGCCG TGTACTCCGA TGCCACGGGC AGTGACCCGC TGTGGCAGCG GCTGGAGCCA 
TGTGGCCACC GAGACAGCGT TTCCTCCTCC TCCAGCATGT CATCCAGCGA CACTGTCATT 
GACCTCTCCC TGCCCAGCCT GGGCCTGGGC CGCAGCCGTG AGAACCTCGC TGGAGCCCAC 
ATGGGACGCC TGCCCCCCAG GCCCCACTCG GCTTCGGCTG CCCGCCCAGA CCTGCCACCT 
GTGACCAAGA GCAAATCCAA CCCCAACCTT CGGGCTACAG GCCAGCGGCC TCCCATACCT 
GACGAACTGC AGCCCAGGTC CCTGGCCCCA AGGATGGCTG GCCTCCCCTT CCGGCCTCCC 
TGGGGCTGCC TTTCCCTGGT GGGCGTGCAG GACTGCCCCG TGGCTGCCAA GTCCAAGAGC 
CTGGGCGACC TCACTGCTGA TGACTTTGCC CCTAGCTTTG AGGGCGGCTC CCGCAGACTG 
AGCCACAGCC TGGGCCTCCC GGGAGGGACA CGGCGGGTGT CGGGGCCAGG GGTGAGACGG 
GACACCCTGA CAGAGCAGCT GCGCTGGCTC ACTGTCTTCC AGCAGGCAGG AGACATCACG 
TCACCCACCA GCCTGGGCCC GGCTGGGGAG GGGGTGGCAG GGGGCCCTGG TTTTGTGCGG 
CGCTCCTCCT CCCGCAGCCA CAGCCGCGTG CGTGCCATTG CCAGCCGGGC CCGCCAGGCC 
CAGGAGCGGC AGCAGAGACT GCAGGGCCTG GGCCGGCAGG GACCCCCAGA AGAGGAGCGG 
GGCACCCCCG AGGGCGCCTG CTCCGTGGGC CACGAGGGCA GTGTGGATGC ACCAGCACCC 
TCCAAGGGAG CCCTCGGGCC AGCATCCGCG GCTGCTGAAA ACCTGGTCCT GCTCCGCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Nature491(7422)119-24(2012 Nov)
Jostins L,Ripke S,Weersma RK,Duerr RH,McGovern DP,Hui KY,Lee JC,Schumm LP,Sharma Y,Anderson CA,Essers J,Mitrovic M,Ning K,Cleynen I,Theatre E,Spain SL,Raychaudhuri S,Goyette P,Wei Z,Abraham C,Achkar JP,Ahmad T,Amininejad L,Ananthakrishnan AN,Andersen V,Andrews JM,Baidoo L,Balschun T,Bampton PA,Bitton A,Boucher G,Brand S,B?ning C,Cohain A,Cichon S,D'Amato M,De Jong D,Devaney KL,Dubinsky M,Edwards C,Ellinghaus D,Ferguson LR,Franchimont D,Fransen K,Gearry R,Georges M,Gieger C,Glas J,Haritunians T,Hart A,Hawkey C,Hedl M,Hu X,Karlsen TH,Kupcinskas L,Kugathasan S,Latiano A,Laukens D,Lawrance IC,Lees CW,Louis E,Mahy G,Mansfield J,Morgan AR,Mowat C,Newman W,Palmieri O,Ponsioen CY,Potocnik U,Prescott NJ,Regueiro M,Rotter JI,Russell RK,Sanderson JD,Sans M,Satsangi J,Schreiber S,Simms LA,Sventoraityte J,Targan SR,Taylor KD,Tremelling M,Verspaget HW,De Vos M,Wijmenga C,Wilson DC,Winkelmann J,Xavier RJ,Zeissig S,Zhang B,Zhang CK,Zhao H,,Silverberg MS,Annese V,Hakonarson H,Brant SR,Radford-Smith G,Mathew CG,Rioux JD,Schadt EE,Daly MJ,Franke A,Parkes M,Vermeire S,Barrett JC,Cho JH

A genome-wide association study in Chinese men identifies three risk loci for non-obstructive azoospermia.
Nature genetics44(2)183-6(2011 Dec)
Hu Z,Xia Y,Guo X,Dai J,Li H,Hu H,Jiang Y,Lu F,Wu Y,Yang X,Li H,Yao B,Lu C,Xiong C,Li Z,Gui Y,Liu J,Zhou Z,Shen H,Wang X,Sha J

Meta-analysis identifies 29 additional ulcerative colitis risk loci, increasing the number of confirmed associations to 47.
Nature genetics43(3)246-52(2011 Mar)
Anderson CA,Boucher G,Lees CW,Franke A,D'Amato M,Taylor KD,Lee JC,Goyette P,Imielinski M,Latiano A,Lagac? C,Scott R,Amininejad L,Bumpstead S,Baidoo L,Baldassano RN,Barclay M,Bayless TM,Brand S,B?ning C,Colombel JF,Denson LA,De Vos M,Dubinsky M,Edwards C,Ellinghaus D,Fehrmann RS,Floyd JA,Florin T,Franchimont D,Franke L,Georges M,Glas J,Glazer NL,Guthery SL,Haritunians T,Hayward NK,Hugot JP,Jobin G,Laukens D,Lawrance I,L?mann M,Levine A,Libioulle C,Louis E,McGovern DP,Milla M,Montgomery GW,Morley KI,Mowat C,Ng A,Newman W,Ophoff RA,Papi L,Palmieri O,Peyrin-Biroulet L,Pan?s J,Phillips A,Prescott NJ,Proctor DD,Roberts R,Russell R,Rutgeerts P,Sanderson J,Sans M,Schumm P,Seibold F,Sharma Y,Simms LA,Seielstad M,Steinhart AH,Targan SR,van den Berg LH,Vatn M,Verspaget H,Walters T,Wijmenga C,Wilson DC,Westra HJ,Xavier RJ,Zhao ZZ,Ponsioen CY,Andersen V,Torkvist L,Gazouli M,Anagnou NP,Karlsen TH,Kupcinskas L,Sventoraityte J,Mansfield JC,Kugathasan S,Silverberg MS,Halfvarson J,Rotter JI,Mathew CG,Griffiths AM,Gearry R,Ahmad T,Brant SR,Chamaillard M,Satsangi J,Cho JH,Schreiber S,Daly MJ,Barrett JC,Parkes M,Annese V,Hakonarson H,Radford-Smith G,Duerr RH,Vermeire S,Weersma RK,Rioux JD

Role of phosphoinositide-specific phospholipase C ?2 in isolated and syndromic mental retardation.
European neurology65(5)264-9(2011)
Lo Vasco VR

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PLCH2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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