kif21a gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012958728.3	XP_012814182.1	kinesin-like protein KIF21A isoform X2
XM_012958731.2	XP_012814185.1	kinesin-like protein KIF21A isoform X5
XM_018091988.1	XP_017947477.1	kinesin-like protein KIF21A isoform X7
XM_012958732.2	XP_012814186.1	kinesin-like protein KIF21A isoform X6
NM_001195664.2	NP_001182593.1	kinesin-like protein KIF21A
XM_012958731.3	XP_012814185.1	kinesin-like protein KIF21A isoform X5
XM_012958729.3	XP_012814183.1	kinesin-like protein KIF21A isoform X3
XM_012958730.3	XP_012814184.1	kinesin-like protein KIF21A isoform X4
XM_012958732.3	XP_012814186.1	kinesin-like protein KIF21A isoform X7
XM_012958727.2	XP_012814181.1	kinesin-like protein KIF21A isoform X1
XM_012958728.2	XP_012814182.1	kinesin-like protein KIF21A isoform X2
XM_012958729.2	XP_012814183.1	kinesin-like protein KIF21A isoform X3
XM_012958730.2	XP_012814184.1	kinesin-like protein KIF21A isoform X4
XM_012958727.3	XP_012814181.1	kinesin-like protein KIF21A isoform X1
XM_031897750.1	XP_031753610.1	kinesin-like protein KIF21A isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43065	XM_018091988.1 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa33415	XM_012958727.3 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa33416	XM_012958728.3 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa33417	XM_012958729.3 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa33418	XM_012958730.3 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa33419	XM_012958731.3 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa33420	XM_012958732.3 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa53391	XM_031897750.1 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa20425	NM_001195664.2 Latest version!	Xenopus tropicalis kinesin family member 21A (kif21a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OXa33419	XM_012958731.2	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa33420	XM_012958732.2	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa33416	XM_012958728.2	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa33417	XM_012958729.2	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa33418	XM_012958730.2	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa33415	XM_012958727.2	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

kif21a ( XM_018091988.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947477.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa43065
Clone ID Related Accession (Same CDS sequence)	XM_018091988.1
Accession Version	XM_018091988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GGTCATCTGG GCAAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947477.1
CDS	233..4273
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKGHLGKS

Target ORF information:

RefSeq Version	XM_018091988.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091988.1

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GGTCATCTGG GCAAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958727.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814181.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa33415
Clone ID Related Accession (Same CDS sequence)	XM_012958727.2 , XM_012958727.3
Accession Version	XM_012958727.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814181.1
CDS	271..5220
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958727.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958727.3

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958728.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814182.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.

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CloneID	OXa33416
Clone ID Related Accession (Same CDS sequence)	XM_012958728.3 , XM_012958728.2
Accession Version	XM_012958728.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG 
GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA 
GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA 
ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA 
AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT 
CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG 
TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG 
GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG 
CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG 
CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA 
GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA 
GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG 
TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA 
CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA 
AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG 
AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA 
TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA 
GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG 
TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC 
CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT 
GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT 
GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT 
CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT 
CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT 
CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC 
TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA 
TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT 
GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC 
TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT 
TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT 
ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA 
AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA 
GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT 
GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA 
AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG 
TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT 
GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC 
AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT 
ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC 
AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA 
TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA 
TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT 
CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT 
GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA 
TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA 
GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG 
GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA 
GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA 
TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG 
TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT 
CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT 
CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT 
GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG 
AGTGAAGATG TAGCCAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814182.1
CDS	271..5214
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958728.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958728.3

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG 
GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA 
GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA 
ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA 
AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT 
CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG 
TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG 
GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG 
CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG 
CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA 
GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA 
GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG 
TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA 
CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA 
AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG 
AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA 
TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA 
GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG 
TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC 
CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT 
GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT 
GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT 
CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT 
CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT 
CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC 
TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA 
TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT 
GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC 
TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT 
TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT 
ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA 
AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA 
GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT 
GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA 
AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG 
TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT 
GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC 
AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT 
ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC 
AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA 
TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA 
TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT 
CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT 
GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA 
TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA 
GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG 
GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA 
GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA 
TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG 
TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT 
CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT 
CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT 
GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG 
AGTGAAGATG TAGCCAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958729.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814183.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.

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CloneID	OXa33417
Clone ID Related Accession (Same CDS sequence)	XM_012958729.3 , XM_012958729.2
Accession Version	XM_012958729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT 
TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT 
AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT 
GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG 
AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT 
GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG 
TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG 
ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT 
CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT 
TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT 
GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA 
GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC 
AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT 
GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC 
AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA 
GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT 
GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT 
TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG 
GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814183.1
CDS	271..5199
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958729.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958729.3

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT 
TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT 
AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT 
GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG 
AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT 
GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG 
TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG 
ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT 
CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT 
TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT 
GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA 
GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC 
AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT 
GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC 
AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA 
GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT 
GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT 
TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG 
GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958730.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814184.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.

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CloneID	OXa33418
Clone ID Related Accession (Same CDS sequence)	XM_012958730.3 , XM_012958730.2
Accession Version	XM_012958730.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA 
TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT 
CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA 
GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC 
ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT 
GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT 
GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA 
CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC 
CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT 
ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC 
ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT 
GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT 
GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA 
GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC 
CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT 
ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC 
ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT 
GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814184.1
CDS	271..5181
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958730.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958730.3

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA 
TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT 
CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA 
GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC 
ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT 
GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT 
GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA 
CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC 
CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT 
ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC 
ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT 
GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT 
GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA 
GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC 
CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT 
ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC 
ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT 
GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958731.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814185.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.

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CloneID	OXa33419
Clone ID Related Accession (Same CDS sequence)	XM_012958731.2 , XM_012958731.3
Accession Version	XM_012958731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814185.1
CDS	270..5159
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958731.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958731.3

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958732.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814186.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.

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CloneID	OXa33420
Clone ID Related Accession (Same CDS sequence)	XM_012958732.2 , XM_012958732.3
Accession Version	XM_012958732.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC 
GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT 
ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC 
ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG 
TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA 
GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT 
ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA 
AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA 
ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG 
TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT 
CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG 
TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT 
CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA 
GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC 
GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC 
GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA 
ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA 
GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG 
TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814186.1
CDS	271..5052
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKLGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958732.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958732.3

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC 
GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT 
ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC 
ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG 
TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA 
GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT 
ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA 
AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA 
ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG 
TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT 
CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG 
TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT 
CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA 
GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC 
GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC 
GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA 
ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA 
GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG 
TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_031897750.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753610.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa53391
Clone ID Related Accession (Same CDS sequence)	XM_031897750.1
Accession Version	XM_031897750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGTCT 
GATGAAAGTG ATTCCTCTAT GTCCGAGGTA CACAGGGGCG TTATTAACCC ATTTCCTGCA 
GCTAAAAGCA GTAAGACATC ACTTCTACAA TGCATTCATA TAGCTGAAGG GCACAACAAG 
GCTGTGCTGT GTGTGGACGC TACTGATGAT CTATTATTCA CTGGTTCCAA AGATCGTACT 
TGTAAAGTAT GGAATCTTGT TACTGGGCAA GAAATTATGT CCCTTGGAGG CCACCCCAAT 
AATGTTGTGT CTGTAAAGTA TTGTAACTAC ACAAGCCTAG TGTTTACTGT TTCCACATCT 
TACATTAAGG TGTGGGACAT CAGAGACTCT GCTAAATGTA TTCGAACACT TACGTCATCT 
GGGCAAGTCA TGACTTGGGA TTCTTGTGCT GGAAGTCAAA GCAGGACCGT AACCATTCCA 
CCGGGTGAAA ATCAAATCAA TCAAATTGCA CTAAACCCAA CAGGCACTTT CTTATATGCA 
GCAGCTGGAA ATTCTGTGAG AATGTGGGAC CTTAAAAGGT TTCAATCAAC AGGGAAATTA 
ACTGGACATC TTGGGCCCGT TATGTGTCTT ACTGTGGATC AGAGTGCCAA TGGGCAAGAC 
CTAATTATTT CAGGCTCAAA GGACCATTAC ATTAAGATGT TTGACATTAC AGAAGGGGCT 
TTGGGTTCTG TCAGTCCCAC ATATTGTTTT GAACCGCCTC ATTATGATGG TATTGAAGCT 
CTAATCATCA GTGGAGATAA CTTGTTTAGT GGATCCCGAG ACAATGGAAT TAAGAAATGG 
GATTTATCAC GCAAAGACCT TCTACAGCAA GTACCAAACG CACACAAAGA CTGGGTGTGT 
GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC CTTTTGAGCG GCTGTCGTGG AGGCATTCTG 
AAACTTTGGA ATGTGGACAC ATTCTTTGCT ATTGGAGAAA TCAAAGGACA TGATAGTCCC 
ATTAATGCAA TTTGTACAAA TTCGAGTCAC ATTTTCACAG CATCAGATGA TCAGACTGTA 
CGAATTTGGA AGGCTCATGG TTCATTGGAT GGACAGATGT CGGATACTGG CGATACGAGT 
GAAGATGTAG CCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753610.1
CDS	271..5091
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKLSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_031897750.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897750.1

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGTCT 
GATGAAAGTG ATTCCTCTAT GTCCGAGGTA CACAGGGGCG TTATTAACCC ATTTCCTGCA 
GCTAAAAGCA GTAAGACATC ACTTCTACAA TGCATTCATA TAGCTGAAGG GCACAACAAG 
GCTGTGCTGT GTGTGGACGC TACTGATGAT CTATTATTCA CTGGTTCCAA AGATCGTACT 
TGTAAAGTAT GGAATCTTGT TACTGGGCAA GAAATTATGT CCCTTGGAGG CCACCCCAAT 
AATGTTGTGT CTGTAAAGTA TTGTAACTAC ACAAGCCTAG TGTTTACTGT TTCCACATCT 
TACATTAAGG TGTGGGACAT CAGAGACTCT GCTAAATGTA TTCGAACACT TACGTCATCT 
GGGCAAGTCA TGACTTGGGA TTCTTGTGCT GGAAGTCAAA GCAGGACCGT AACCATTCCA 
CCGGGTGAAA ATCAAATCAA TCAAATTGCA CTAAACCCAA CAGGCACTTT CTTATATGCA 
GCAGCTGGAA ATTCTGTGAG AATGTGGGAC CTTAAAAGGT TTCAATCAAC AGGGAAATTA 
ACTGGACATC TTGGGCCCGT TATGTGTCTT ACTGTGGATC AGAGTGCCAA TGGGCAAGAC 
CTAATTATTT CAGGCTCAAA GGACCATTAC ATTAAGATGT TTGACATTAC AGAAGGGGCT 
TTGGGTTCTG TCAGTCCCAC ATATTGTTTT GAACCGCCTC ATTATGATGG TATTGAAGCT 
CTAATCATCA GTGGAGATAA CTTGTTTAGT GGATCCCGAG ACAATGGAAT TAAGAAATGG 
GATTTATCAC GCAAAGACCT TCTACAGCAA GTACCAAACG CACACAAAGA CTGGGTGTGT 
GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC CTTTTGAGCG GCTGTCGTGG AGGCATTCTG 
AAACTTTGGA ATGTGGACAC ATTCTTTGCT ATTGGAGAAA TCAAAGGACA TGATAGTCCC 
ATTAATGCAA TTTGTACAAA TTCGAGTCAC ATTTTCACAG CATCAGATGA TCAGACTGTA 
CGAATTTGGA AGGCTCATGG TTCATTGGAT GGACAGATGT CGGATACTGG CGATACGAGT 
GAAGATGTAG CCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( NM_001195664.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001182593.1 Xenopus tropicalis kinesin family member 21A (kif21a), mRNA.

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CloneID	OXa20425
Clone ID Related Accession (Same CDS sequence)	NM_001195664.2
Accession Version	NM_001195664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-06
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03011706.1. On Aug 10, 2013 this sequence version replaced NM_001195664.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGTACAG AATTCTTCTT 
TGTAGAACTT GTTCCCATCG CAAGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT 
AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT 
GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG 
AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT 
GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG 
TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG 
ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT 
CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT 
TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT 
GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA 
GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC 
AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT 
GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC 
AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA 
GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT 
GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT 
TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG 
GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182593.1
CDS	233..5221
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRYRILLCRTCSHRKGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	NM_001195664.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195664.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGTACAG AATTCTTCTT 
TGTAGAACTT GTTCCCATCG CAAGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT 
AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT 
GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG 
AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT 
GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG 
TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG 
ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT 
CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT 
TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT 
GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA 
GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC 
AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT 
GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC 
AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA 
GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT 
GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT 
TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG 
GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958727.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814181.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.

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CloneID	OXa33415
Clone ID Related Accession (Same CDS sequence)	XM_012958727.2 , XM_012958727.3
Accession Version	XM_012958727.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814181.1
CDS	295..5244
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958727.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958727.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958728.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814182.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.

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CloneID	OXa33416
Clone ID Related Accession (Same CDS sequence)	XM_012958728.3 , XM_012958728.2
Accession Version	XM_012958728.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG 
GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA 
GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA 
ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA 
AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT 
CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG 
TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG 
GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG 
CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG 
CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA 
GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA 
GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG 
TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA 
CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA 
AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG 
AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA 
TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA 
GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG 
TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC 
CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT 
GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT 
GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT 
CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT 
CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT 
CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC 
TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA 
TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT 
GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC 
TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT 
TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT 
ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA 
AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA 
GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT 
GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA 
AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG 
TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT 
GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC 
AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT 
ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC 
AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA 
TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA 
TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT 
CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT 
GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA 
TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA 
GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG 
GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA 
GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA 
TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG 
TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT 
CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT 
CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT 
GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG 
AGTGAAGATG TAGCCAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814182.1
CDS	295..5238
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958728.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958728.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG 
GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA 
GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA 
ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA 
AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT 
CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG 
TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG 
GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG 
CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG 
CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA 
GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA 
GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG 
TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA 
CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA 
AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG 
AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA 
TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA 
GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG 
TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC 
CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT 
GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT 
GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT 
CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT 
CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT 
CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC 
TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA 
TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT 
GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC 
TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT 
TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT 
ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA 
AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA 
GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT 
GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA 
AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG 
TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT 
GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC 
AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT 
ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC 
AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA 
TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA 
TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT 
CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT 
GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA 
TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA 
GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG 
GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA 
GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA 
TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG 
TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT 
CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT 
CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT 
GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG 
AGTGAAGATG TAGCCAGCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958729.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814183.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.

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CloneID	OXa33417
Clone ID Related Accession (Same CDS sequence)	XM_012958729.3 , XM_012958729.2
Accession Version	XM_012958729.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT 
TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT 
AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT 
GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG 
AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT 
GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG 
TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG 
ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT 
CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT 
TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT 
GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA 
GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC 
AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT 
GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC 
AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA 
GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT 
GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT 
TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG 
GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814183.1
CDS	295..5223
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKSDESDSSMSEVHRGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958729.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958729.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT 
TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT 
AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT 
GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG 
AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT 
GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG 
TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG 
ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT 
CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT 
TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT 
GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA 
GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC 
AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT 
GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC 
AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA 
GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT 
GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT 
TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG 
GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC 
AGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958730.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814184.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.

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CloneID	OXa33418
Clone ID Related Accession (Same CDS sequence)	XM_012958730.3 , XM_012958730.2
Accession Version	XM_012958730.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA 
TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT 
CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA 
GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC 
ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT 
GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT 
GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA 
CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC 
CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT 
ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC 
ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT 
GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT 
GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA 
GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC 
CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT 
ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC 
ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT 
GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814184.1
CDS	295..5205
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958730.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958730.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA 
GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT 
AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG 
GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA 
TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT 
CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA 
GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC 
ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT 
GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT 
GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA 
CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC 
CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT 
ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC 
ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT 
GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT 
GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA 
GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC 
CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT 
ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC 
ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT 
GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958731.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814185.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa33419
Clone ID Related Accession (Same CDS sequence)	XM_012958731.2 , XM_012958731.3
Accession Version	XM_012958731.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814185.1
CDS	294..5183
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKFSDSGTSEASLSPPSSPTSRPRNEKNVFSRLTVSQADTAFQQDKGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958731.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958731.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC 
AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA 
AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC 
GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT 
TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA 
GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG 
ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT 
GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT 
AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC 
CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA 
TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG 
CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA 
GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC 
CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT 
TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT 
ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA 
ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC 
TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA 
GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT 
GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA 
GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT 
ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT 
AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC 
TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA 
GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT 
GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT 
CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC 
GATACGAGTG AAGATGTAGC CAGCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21a ( XM_012958732.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814186.1 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.

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CloneID	OXa33420
Clone ID Related Accession (Same CDS sequence)	XM_012958732.2 , XM_012958732.3
Accession Version	XM_012958732.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC 
GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT 
ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC 
ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG 
TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA 
GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT 
ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA 
AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA 
ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG 
TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT 
CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG 
TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT 
CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA 
GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC 
GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC 
GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA 
ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA 
GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG 
TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814186.1
CDS	295..5076
Translation	MTGAQDDSAVRVALRIRPQLAKEKIEGCHICTSVTPGEPQVVLGKDKAFTFDYVFDIDSRQEEIYMQCTEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNISEEEHGIIPRAVTHLFRRIEEKKQFALEQGLPPPDFKVNAQFLELYNEEVLDLFDTTRDIDARNKKSNIRIHEDSSGGIYTVGVTTRNVSSETEMIQCLKIGALSRTTASTQMNVQSSRSHAIFTIHLCQNRVCPKIDNENDLDNRMASESNQINEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKKATHVPYRDSKLTRLLQDSLGGNSQTVMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRTSQQINALRNEIARLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETIDALRSRITQLMSDQANQVLSRSGDGNEEISNMIHNYIKEIEDLRAKLLESEAVNENLRKSLSRASSRSSYFPAHTPFPTSLIAAEQETNEIIDMAKKDLEKLKKIEKKKKKSIVKEENPDNEKLEEKDNTEKECHDLVIEERSDHEEDEEEEEEEEDEDVDAAESSDDSDSELEEKENYQADLANITCEIAIKQKLIDELENSQRRLHTLKQQYEEKLMTLQNKIKDTQLERDRVLQNMGSMETCTEEKAKKIKVEYEKKLQTMNKELQKLQAAQKEHARLLKNQSQYEKQMKKLQQEVVEMKKTKVRLMKQMKEEQEKARVTETRRNREIAQLKKDQRKRDSQLRLLEAQKKQQEVVLRRKTEEVTALRRQVRPMSDRVAGRVGRKISLPEPIAEHISNMPEELDSTKHGLQQKPRIPVARVQPATSTALNGTRKKYQRKGVSSYTSKAARQKWQSLERRITDIVMQRMTISNMEVDMNRLLKQREELTKRKEKVSKKRDRILKESGDADKNIHSLSEEMESLNANIDYLNDSISDCQASIMQMEEAKEEGDTLDVTAVISSCTLTEARYLLDHFLNMAVSKSLQAAQREAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSIPLENAENSSDDDGSLQSPSTEGGSLTSDLMKLCGEVKPKSKARRRTTTQLELLYADSSESTPDAGTGETCIPTSLPPVSEMQVSGLNIESSVNTVKEKEMLHSGIPSRTGSISKPVTLTDKRIPEQSPATRRRVYDKGGDKSIKNKEQKLGVINPFPAAKSSKTSLLQCIHIAEGHNKAVLCVDATDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVMTWDSCAGSQSRTVTIPPGENQINQIALNPTGTFLYAAAGNSVRMWDLKRFQSTGKLTGHLGPVMCLTVDQSANGQDLIISGSKDHYIKMFDITEGALGSVSPTYCFEPPHYDGIEALIISGDNLFSGSRDNGIKKWDLSRKDLLQQVPNAHKDWVCALGLVPGCPVLLSGCRGGILKLWNVDTFFAIGEIKGHDSPINAICTNSSHIFTASDDQTVRIWKAHGSLDGQMSDTGDTSEDVASN

Target ORF information:

RefSeq Version	XM_012958732.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958732.2

ORF Insert Sequence:

ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA 
GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG 
CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC 
AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA 
GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT 
CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT 
GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT 
GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT 
TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG 
ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT 
GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC 
CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA 
AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT 
TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG 
CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC 
GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC 
CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC 
ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC 
CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC 
ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC 
ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA 
GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA 
GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC 
AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT 
GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT 
ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG 
GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC 
AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC 
CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG 
GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG 
GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG 
AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT 
GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT 
GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA 
GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC 
CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA 
CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA 
GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG 
AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA 
CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA 
CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT 
GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG 
AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA 
AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA 
TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT 
ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA 
AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT 
CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC 
ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA 
CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT 
CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT 
AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC 
TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT 
CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT 
GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA 
CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG 
TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA 
CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT 
GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC 
ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA 
AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC 
GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT 
ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC 
ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG 
TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA 
GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT 
ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA 
AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA 
ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG 
TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT 
CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG 
TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT 
CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA 
GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC 
GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC 
GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA 
ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA 
GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG 
TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kif21a
Entrez Gene ID	100485713
Full Name	kinesin family member 21A
Synonyms	cfeom1,feom1,feom3a
General protein information	Preferred Names kinesin family member 21A Names kinesin-like protein KIF21A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	kif21a	XP_001920258.4
Canis lupus familiaris (dog)	KIF21A	XP_003433563.1
Mus musculus (house mouse)	Kif21a	NP_001102511.1
Gallus gallus (chicken)	KIF21A	XP_004934816.1
Drosophila melanogaster (fruit fly)	Klp31E	NP_001162937.2
Rattus norvegicus (Norway rat)	Kif21a	XP_006242318.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007815	XP_317685.4
Caenorhabditis elegans (roundworm)	klp-12	NP_001023349.1
Homo sapiens (human)	KIF21A	NP_001166935.1
	KIF21A	XP_002798575.1
Bos taurus (cattle)	KIF21A	NP_001193847.1
Xenopus tropicalis (tropical clawed frog)	kif21a	NP_001182593.1
Pan troglodytes (chimpanzee)	KIF21A	XP_003313814.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kif21a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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