kif21a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kif21a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif21a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa43065 XM_018091988.1
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa33415 XM_012958727.3
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33416 XM_012958728.3
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33417 XM_012958729.3
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33418 XM_012958730.3
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33419 XM_012958731.3
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa33420 XM_012958732.3
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa53391 XM_031897750.1
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa20425 NM_001195664.2
Latest version!
Xenopus tropicalis kinesin family member 21A (kif21a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OXa33419 XM_012958731.2 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33420 XM_012958732.2 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33416 XM_012958728.2 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33417 XM_012958729.2 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33418 XM_012958730.2 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa33415 XM_012958727.2 Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa43065
    Clone ID Related Accession (Same CDS sequence) XM_018091988.1
    Accession Version XM_018091988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GGTCATCTGG GCAAGTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947477.1
    CDS233..4273
    Translation

    Target ORF information:

    RefSeq Version XM_018091988.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GGTCATCTGG GCAAGTCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33415
    Clone ID Related Accession (Same CDS sequence) XM_012958727.2 , XM_012958727.3
    Accession Version XM_012958727.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814181.1
    CDS271..5220
    Translation

    Target ORF information:

    RefSeq Version XM_012958727.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958727.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33416
    Clone ID Related Accession (Same CDS sequence) XM_012958728.3 , XM_012958728.2
    Accession Version XM_012958728.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG
    GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA
    GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA
    ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA
    AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT
    CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG
    TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG
    GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG
    CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG
    CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA
    GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA
    GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG
    TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA
    CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA
    AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG
    AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA
    TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA
    GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG
    TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC
    CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT
    GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT
    GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT
    CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT
    CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT
    CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC
    TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA
    TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT
    GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC
    TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT
    TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT
    ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA
    AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA
    GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT
    GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA
    AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG
    TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT
    GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC
    AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT
    ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC
    AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA
    TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA
    TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT
    CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT
    GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA
    TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA
    GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG
    GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA
    GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA
    TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG
    TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT
    CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT
    CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT
    GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG
    AGTGAAGATG TAGCCAGCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814182.1
    CDS271..5214
    Translation

    Target ORF information:

    RefSeq Version XM_012958728.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958728.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG
    GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA
    GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA
    ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA
    AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT
    CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG
    TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG
    GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG
    CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG
    CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA
    GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA
    GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG
    TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA
    CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA
    AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG
    AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA
    TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA
    GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG
    TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC
    CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT
    GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT
    GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT
    CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT
    CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT
    CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC
    TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA
    TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT
    GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC
    TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT
    TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT
    ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA
    AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA
    GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT
    GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA
    AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG
    TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT
    GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC
    AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT
    ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC
    AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA
    TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA
    TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT
    CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT
    GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA
    TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA
    GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG
    GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA
    GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA
    TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG
    TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT
    CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT
    CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT
    GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG
    AGTGAAGATG TAGCCAGCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33417
    Clone ID Related Accession (Same CDS sequence) XM_012958729.3 , XM_012958729.2
    Accession Version XM_012958729.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT
    TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT
    AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT
    GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG
    AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT
    GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG
    TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG
    ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT
    CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT
    TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT
    GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA
    GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC
    AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT
    GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC
    AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA
    GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT
    GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT
    TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG
    GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC
    AGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814183.1
    CDS271..5199
    Translation

    Target ORF information:

    RefSeq Version XM_012958729.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958729.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT
    TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT
    AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT
    GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG
    AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT
    GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG
    TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG
    ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT
    CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT
    TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT
    GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA
    GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC
    AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT
    GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC
    AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA
    GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT
    GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT
    TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG
    GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC
    AGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33418
    Clone ID Related Accession (Same CDS sequence) XM_012958730.3 , XM_012958730.2
    Accession Version XM_012958730.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA
    TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT
    CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA
    GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC
    ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT
    GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT
    GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA
    CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC
    CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT
    ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC
    ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT
    GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT
    GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA
    GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC
    CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT
    ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC
    ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT
    GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814184.1
    CDS271..5181
    Translation

    Target ORF information:

    RefSeq Version XM_012958730.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958730.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA
    TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT
    CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA
    GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC
    ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT
    GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT
    GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA
    CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC
    CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT
    ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC
    ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT
    GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT
    GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA
    GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC
    CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT
    ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC
    ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT
    GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33419
    Clone ID Related Accession (Same CDS sequence) XM_012958731.2 , XM_012958731.3
    Accession Version XM_012958731.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814185.1
    CDS270..5159
    Translation

    Target ORF information:

    RefSeq Version XM_012958731.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958731.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33420
    Clone ID Related Accession (Same CDS sequence) XM_012958732.2 , XM_012958732.3
    Accession Version XM_012958732.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC
    GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT
    ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC
    ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG
    TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA
    GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT
    ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA
    AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA
    ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG
    TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT
    CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG
    TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT
    CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA
    GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC
    GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC
    GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA
    ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA
    GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG
    TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814186.1
    CDS271..5052
    Translation

    Target ORF information:

    RefSeq Version XM_012958732.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958732.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC
    GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT
    ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC
    ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG
    TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA
    GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT
    ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA
    AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA
    ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG
    TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT
    CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG
    TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT
    CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA
    GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC
    GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC
    GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA
    ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA
    GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG
    TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53391
    Clone ID Related Accession (Same CDS sequence) XM_031897750.1
    Accession Version XM_031897750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGTCT
    GATGAAAGTG ATTCCTCTAT GTCCGAGGTA CACAGGGGCG TTATTAACCC ATTTCCTGCA
    GCTAAAAGCA GTAAGACATC ACTTCTACAA TGCATTCATA TAGCTGAAGG GCACAACAAG
    GCTGTGCTGT GTGTGGACGC TACTGATGAT CTATTATTCA CTGGTTCCAA AGATCGTACT
    TGTAAAGTAT GGAATCTTGT TACTGGGCAA GAAATTATGT CCCTTGGAGG CCACCCCAAT
    AATGTTGTGT CTGTAAAGTA TTGTAACTAC ACAAGCCTAG TGTTTACTGT TTCCACATCT
    TACATTAAGG TGTGGGACAT CAGAGACTCT GCTAAATGTA TTCGAACACT TACGTCATCT
    GGGCAAGTCA TGACTTGGGA TTCTTGTGCT GGAAGTCAAA GCAGGACCGT AACCATTCCA
    CCGGGTGAAA ATCAAATCAA TCAAATTGCA CTAAACCCAA CAGGCACTTT CTTATATGCA
    GCAGCTGGAA ATTCTGTGAG AATGTGGGAC CTTAAAAGGT TTCAATCAAC AGGGAAATTA
    ACTGGACATC TTGGGCCCGT TATGTGTCTT ACTGTGGATC AGAGTGCCAA TGGGCAAGAC
    CTAATTATTT CAGGCTCAAA GGACCATTAC ATTAAGATGT TTGACATTAC AGAAGGGGCT
    TTGGGTTCTG TCAGTCCCAC ATATTGTTTT GAACCGCCTC ATTATGATGG TATTGAAGCT
    CTAATCATCA GTGGAGATAA CTTGTTTAGT GGATCCCGAG ACAATGGAAT TAAGAAATGG
    GATTTATCAC GCAAAGACCT TCTACAGCAA GTACCAAACG CACACAAAGA CTGGGTGTGT
    GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC CTTTTGAGCG GCTGTCGTGG AGGCATTCTG
    AAACTTTGGA ATGTGGACAC ATTCTTTGCT ATTGGAGAAA TCAAAGGACA TGATAGTCCC
    ATTAATGCAA TTTGTACAAA TTCGAGTCAC ATTTTCACAG CATCAGATGA TCAGACTGTA
    CGAATTTGGA AGGCTCATGG TTCATTGGAT GGACAGATGT CGGATACTGG CGATACGAGT
    GAAGATGTAG CCAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753610.1
    CDS271..5091
    Translation

    Target ORF information:

    RefSeq Version XM_031897750.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031897750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGTCT
    GATGAAAGTG ATTCCTCTAT GTCCGAGGTA CACAGGGGCG TTATTAACCC ATTTCCTGCA
    GCTAAAAGCA GTAAGACATC ACTTCTACAA TGCATTCATA TAGCTGAAGG GCACAACAAG
    GCTGTGCTGT GTGTGGACGC TACTGATGAT CTATTATTCA CTGGTTCCAA AGATCGTACT
    TGTAAAGTAT GGAATCTTGT TACTGGGCAA GAAATTATGT CCCTTGGAGG CCACCCCAAT
    AATGTTGTGT CTGTAAAGTA TTGTAACTAC ACAAGCCTAG TGTTTACTGT TTCCACATCT
    TACATTAAGG TGTGGGACAT CAGAGACTCT GCTAAATGTA TTCGAACACT TACGTCATCT
    GGGCAAGTCA TGACTTGGGA TTCTTGTGCT GGAAGTCAAA GCAGGACCGT AACCATTCCA
    CCGGGTGAAA ATCAAATCAA TCAAATTGCA CTAAACCCAA CAGGCACTTT CTTATATGCA
    GCAGCTGGAA ATTCTGTGAG AATGTGGGAC CTTAAAAGGT TTCAATCAAC AGGGAAATTA
    ACTGGACATC TTGGGCCCGT TATGTGTCTT ACTGTGGATC AGAGTGCCAA TGGGCAAGAC
    CTAATTATTT CAGGCTCAAA GGACCATTAC ATTAAGATGT TTGACATTAC AGAAGGGGCT
    TTGGGTTCTG TCAGTCCCAC ATATTGTTTT GAACCGCCTC ATTATGATGG TATTGAAGCT
    CTAATCATCA GTGGAGATAA CTTGTTTAGT GGATCCCGAG ACAATGGAAT TAAGAAATGG
    GATTTATCAC GCAAAGACCT TCTACAGCAA GTACCAAACG CACACAAAGA CTGGGTGTGT
    GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC CTTTTGAGCG GCTGTCGTGG AGGCATTCTG
    AAACTTTGGA ATGTGGACAC ATTCTTTGCT ATTGGAGAAA TCAAAGGACA TGATAGTCCC
    ATTAATGCAA TTTGTACAAA TTCGAGTCAC ATTTTCACAG CATCAGATGA TCAGACTGTA
    CGAATTTGGA AGGCTCATGG TTCATTGGAT GGACAGATGT CGGATACTGG CGATACGAGT
    GAAGATGTAG CCAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa20425
    Clone ID Related Accession (Same CDS sequence) NM_001195664.2
    Accession Version NM_001195664.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-06
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03011706.1. On Aug 10, 2013 this sequence version replaced NM_001195664.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGTACAG AATTCTTCTT
    TGTAGAACTT GTTCCCATCG CAAGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT
    AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT
    GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG
    AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT
    GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG
    TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG
    ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT
    CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT
    TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT
    GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA
    GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC
    AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT
    GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC
    AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA
    GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT
    GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT
    TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG
    GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC
    AGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001182593.1
    CDS233..5221
    Translation

    Target ORF information:

    RefSeq Version NM_001195664.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001195664.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGTACAG AATTCTTCTT
    TGTAGAACTT GTTCCCATCG CAAGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT
    AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT
    GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG
    AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT
    GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG
    TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG
    ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT
    CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT
    TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT
    GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA
    GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC
    AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT
    GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC
    AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA
    GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT
    GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT
    TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG
    GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC
    AGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33415
    Clone ID Related Accession (Same CDS sequence) XM_012958727.2 , XM_012958727.3
    Accession Version XM_012958727.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814181.1
    CDS295..5244
    Translation

    Target ORF information:

    RefSeq Version XM_012958727.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958727.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGTCTG ATGAAAGTGA TTCCTCTATG TCCGAGGTAC ACAGGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33416
    Clone ID Related Accession (Same CDS sequence) XM_012958728.3 , XM_012958728.2
    Accession Version XM_012958728.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958728.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG
    GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA
    GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA
    ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA
    AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT
    CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG
    TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG
    GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG
    CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG
    CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA
    GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA
    GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG
    TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA
    CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA
    AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG
    AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA
    TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA
    GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG
    TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC
    CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT
    GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT
    GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT
    CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT
    CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT
    CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC
    TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA
    TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT
    GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC
    TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT
    TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT
    ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA
    AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA
    GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT
    GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA
    AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG
    TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT
    GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC
    AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT
    ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC
    AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA
    TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA
    TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT
    CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT
    GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA
    TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA
    GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG
    GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA
    GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA
    TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG
    TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT
    CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT
    CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT
    GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG
    AGTGAAGATG TAGCCAGCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814182.1
    CDS295..5238
    Translation

    Target ORF information:

    RefSeq Version XM_012958728.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958728.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG AGGAAAGGAG TGACCATGAG GAAGATGAAG AGGAAGAAGA GGAGGAAGAG
    GATGAAGATG TAGATGCTGC TGAAAGTTCA GATGATTCAG ATTCTGAACT GGAGGAAAAA
    GAGAATTACC AGGCTGATCT GGCAAATATT ACTTGTGAAA TTGCTATAAA GCAGAAGCTA
    ATTGATGAAT TAGAAAACAG CCAGCGGAGA CTGCATACAC TAAAACAGCA GTATGAAGAA
    AAACTGATGA CTCTGCAAAA TAAAATAAAG GACACTCAAC TAGAAAGAGA TCGTGTTCTT
    CAAAACATGG GATCAATGGA AACATGCACA GAGGAAAAAG CAAAAAAAAT TAAAGTGGAG
    TATGAAAAGA AACTGCAGAC AATGAATAAG GAACTTCAGA AACTTCAGGC TGCCCAAAAG
    GAACATGCTC GTTTGCTGAA AAACCAGTCA CAGTATGAAA AACAAATGAA GAAACTGCAG
    CAGGAGGTTG TAGAAATGAA GAAAACAAAG GTTCGTCTAA TGAAACAGAT GAAAGAAGAG
    CAAGAAAAAG CAAGAGTAAC TGAAACCCGC AGAAACAGAG AAATTGCACA GCTGAAAAAA
    GACCAGCGTA AACGAGATAG TCAGTTACGA CTTTTAGAAG CCCAGAAAAA ACAACAAGAA
    GTTGTGCTGC GTCGTAAAAC TGAAGAGGTT ACAGCACTTC GTCGCCAAGT AAGACCCATG
    TCTGATAGGG TTGCAGGAAG GGTAGGCCGT AAAATAAGTT TGCCTGAGCC TATTGCCGAA
    CATATTTCAA ATATGCCTGA GGAACTGGAT TCAACCAAAC ATGGGTTACA GCAGAAACCA
    AGAATTCCTG TTGCCAGAGT CCAGCCTGCC ACGTCAACTG CCTTAAATGG GACAAGGAAG
    AAATATCAGA GAAAAGGAGT AAGTAGTTAC ACTTCAAAGG CAGCCAGACA GAAATGGCAA
    TCACTGGAGC GCAGAATAAC AGACATTGTG ATGCAGAGAA TGACTATTTC CAATATGGAA
    GTTGATATGA ACAGACTACT AAAGCAACGG GAGGAACTCA CAAAAAGGAA GGAAAAGGTG
    TCAAAAAAAA GAGATCGCAT TTTAAAGGAA TCTGGAGATG CAGACAAAAA TATTCATAGC
    CTCAGTGAAG AAATGGAGTC CCTTAATGCA AATATTGACT ACCTTAATGA TAGCATATCT
    GACTGCCAAG CTAGCATTAT GCAAATGGAG GAGGCAAAGG AAGAAGGTGA TACACTGGAT
    GTAACAGCAG TGATTAGTTC CTGCACTTTA ACTGAAGCAC GTTATCTTCT TGATCATTTT
    CTGAACATGG CTGTTAGTAA GAGTCTTCAA GCAGCACAAA GAGAGGCTCA AATTAAAGTT
    CTTGAAGGAC GTCTTAAACA AACAGAAATA ACAAGTGCAA CCCAAAATCA GCTCTTGTTT
    CATATGTTAA AAGAAAAAGC AGAACTAAAT CCAGAACTTG ATGCGCTTCT TGGTCATGCC
    TTACAAGATC TAGATAGCAT ACCGCTAGAA AATGCAGAAA ACAGCAGTGA TGATGATGGA
    TCATTACAGA GTCCCAGTAC AGAAGGCGGT TCCTTGACCT CTGATCTCAT GAAACTGTGT
    GGTGAAGTCA AGCCAAAAAG CAAGGCACGG AGAAGAACCA CAACCCAGCT TGAGTTACTC
    TATGCAGACA GCAGCGAGTC TACTCCAGAT GCTGGCACTG GAGAGACATG TATACCAACT
    TCCCTCCCCC CTGTTTCAGA AATGCAAGTG TCTGGGCTGA ATATAGAATC AAGTGTCAAT
    ACTGTTAAGG AGAAAGAGAT GCTGCATTCT GGCATACCTT CTAGAACAGG CAGCATATCA
    AAACCGGTAA CTTTGACTGA CAAAAGAATT CCAGAACAAT CACCAGCAAC AAGAAGGAGA
    GTATATGACA AAGGTGGTGA TAAATCAATA AAAAACAAAG AACAAAAGTT CTCAGATTCT
    GGAACCTCGG AGGCTAGTTT GTCTCCTCCT TCTTCCCCAA CAAGCCGGCC CCGTAATGAA
    AAGAATGTTT TTAGCCGTCT CACAGTTTCT CAGGCAGACA CAGCATTTCA GCAGGATAAG
    TCTGATGAAA GTGATTCCTC TATGTCCGAG GTACACAGGG GCGTTATTAA CCCATTTCCT
    GCAGCTAAAA GCAGTAAGAC ATCACTTCTA CAATGCATTC ATATAGCTGA AGGGCACAAC
    AAGGCTGTGC TGTGTGTGGA CGCTACTGAT GATCTATTAT TCACTGGTTC CAAAGATCGT
    ACTTGTAAAG TATGGAATCT TGTTACTGGG CAAGAAATTA TGTCCCTTGG AGGCCACCCC
    AATAATGTTG TGTCTGTAAA GTATTGTAAC TACACAAGCC TAGTGTTTAC TGTTTCCACA
    TCTTACATTA AGGTGTGGGA CATCAGAGAC TCTGCTAAAT GTATTCGAAC ACTTACGTCA
    TCTGGGCAAG TCATGACTTG GGATTCTTGT GCTGGAAGTC AAAGCAGGAC CGTAACCATT
    CCACCGGGTG AAAATCAAAT CAATCAAATT GCACTAAACC CAACAGGCAC TTTCTTATAT
    GCAGCAGCTG GAAATTCTGT GAGAATGTGG GACCTTAAAA GGTTTCAATC AACAGGGAAA
    TTAACTGGAC ATCTTGGGCC CGTTATGTGT CTTACTGTGG ATCAGAGTGC CAATGGGCAA
    GACCTAATTA TTTCAGGCTC AAAGGACCAT TACATTAAGA TGTTTGACAT TACAGAAGGG
    GCTTTGGGTT CTGTCAGTCC CACATATTGT TTTGAACCGC CTCATTATGA TGGTATTGAA
    GCTCTAATCA TCAGTGGAGA TAACTTGTTT AGTGGATCCC GAGACAATGG AATTAAGAAA
    TGGGATTTAT CACGCAAAGA CCTTCTACAG CAAGTACCAA ACGCACACAA AGACTGGGTG
    TGTGCCCTCG GTTTAGTGCC TGGTTGCCCT GTCCTTTTGA GCGGCTGTCG TGGAGGCATT
    CTGAAACTTT GGAATGTGGA CACATTCTTT GCTATTGGAG AAATCAAAGG ACATGATAGT
    CCCATTAATG CAATTTGTAC AAATTCGAGT CACATTTTCA CAGCATCAGA TGATCAGACT
    GTACGAATTT GGAAGGCTCA TGGTTCATTG GATGGACAGA TGTCGGATAC TGGCGATACG
    AGTGAAGATG TAGCCAGCAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33417
    Clone ID Related Accession (Same CDS sequence) XM_012958729.3 , XM_012958729.2
    Accession Version XM_012958729.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT
    TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT
    AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT
    GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG
    AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT
    GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG
    TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG
    ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT
    CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT
    TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT
    GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA
    GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC
    AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT
    GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC
    AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA
    GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT
    GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT
    TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG
    GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC
    AGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814183.1
    CDS295..5223
    Translation

    Target ORF information:

    RefSeq Version XM_012958729.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958729.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGTCTGA TGAAAGTGAT
    TCCTCTATGT CCGAGGTACA CAGGGGCGTT ATTAACCCAT TTCCTGCAGC TAAAAGCAGT
    AAGACATCAC TTCTACAATG CATTCATATA GCTGAAGGGC ACAACAAGGC TGTGCTGTGT
    GTGGACGCTA CTGATGATCT ATTATTCACT GGTTCCAAAG ATCGTACTTG TAAAGTATGG
    AATCTTGTTA CTGGGCAAGA AATTATGTCC CTTGGAGGCC ACCCCAATAA TGTTGTGTCT
    GTAAAGTATT GTAACTACAC AAGCCTAGTG TTTACTGTTT CCACATCTTA CATTAAGGTG
    TGGGACATCA GAGACTCTGC TAAATGTATT CGAACACTTA CGTCATCTGG GCAAGTCATG
    ACTTGGGATT CTTGTGCTGG AAGTCAAAGC AGGACCGTAA CCATTCCACC GGGTGAAAAT
    CAAATCAATC AAATTGCACT AAACCCAACA GGCACTTTCT TATATGCAGC AGCTGGAAAT
    TCTGTGAGAA TGTGGGACCT TAAAAGGTTT CAATCAACAG GGAAATTAAC TGGACATCTT
    GGGCCCGTTA TGTGTCTTAC TGTGGATCAG AGTGCCAATG GGCAAGACCT AATTATTTCA
    GGCTCAAAGG ACCATTACAT TAAGATGTTT GACATTACAG AAGGGGCTTT GGGTTCTGTC
    AGTCCCACAT ATTGTTTTGA ACCGCCTCAT TATGATGGTA TTGAAGCTCT AATCATCAGT
    GGAGATAACT TGTTTAGTGG ATCCCGAGAC AATGGAATTA AGAAATGGGA TTTATCACGC
    AAAGACCTTC TACAGCAAGT ACCAAACGCA CACAAAGACT GGGTGTGTGC CCTCGGTTTA
    GTGCCTGGTT GCCCTGTCCT TTTGAGCGGC TGTCGTGGAG GCATTCTGAA ACTTTGGAAT
    GTGGACACAT TCTTTGCTAT TGGAGAAATC AAAGGACATG ATAGTCCCAT TAATGCAATT
    TGTACAAATT CGAGTCACAT TTTCACAGCA TCAGATGATC AGACTGTACG AATTTGGAAG
    GCTCATGGTT CATTGGATGG ACAGATGTCG GATACTGGCG ATACGAGTGA AGATGTAGCC
    AGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33418
    Clone ID Related Accession (Same CDS sequence) XM_012958730.3 , XM_012958730.2
    Accession Version XM_012958730.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA
    TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT
    CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA
    GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC
    ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT
    GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT
    GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA
    CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC
    CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT
    ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC
    ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT
    GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT
    GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA
    GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC
    CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT
    ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC
    ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT
    GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814184.1
    CDS295..5205
    Translation

    Target ORF information:

    RefSeq Version XM_012958730.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958730.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTCTCA
    GATTCTGGAA CCTCGGAGGC TAGTTTGTCT CCTCCTTCTT CCCCAACAAG CCGGCCCCGT
    AATGAAAAGA ATGTTTTTAG CCGTCTCACA GTTTCTCAGG CAGACACAGC ATTTCAGCAG
    GATAAGGGCG TTATTAACCC ATTTCCTGCA GCTAAAAGCA GTAAGACATC ACTTCTACAA
    TGCATTCATA TAGCTGAAGG GCACAACAAG GCTGTGCTGT GTGTGGACGC TACTGATGAT
    CTATTATTCA CTGGTTCCAA AGATCGTACT TGTAAAGTAT GGAATCTTGT TACTGGGCAA
    GAAATTATGT CCCTTGGAGG CCACCCCAAT AATGTTGTGT CTGTAAAGTA TTGTAACTAC
    ACAAGCCTAG TGTTTACTGT TTCCACATCT TACATTAAGG TGTGGGACAT CAGAGACTCT
    GCTAAATGTA TTCGAACACT TACGTCATCT GGGCAAGTCA TGACTTGGGA TTCTTGTGCT
    GGAAGTCAAA GCAGGACCGT AACCATTCCA CCGGGTGAAA ATCAAATCAA TCAAATTGCA
    CTAAACCCAA CAGGCACTTT CTTATATGCA GCAGCTGGAA ATTCTGTGAG AATGTGGGAC
    CTTAAAAGGT TTCAATCAAC AGGGAAATTA ACTGGACATC TTGGGCCCGT TATGTGTCTT
    ACTGTGGATC AGAGTGCCAA TGGGCAAGAC CTAATTATTT CAGGCTCAAA GGACCATTAC
    ATTAAGATGT TTGACATTAC AGAAGGGGCT TTGGGTTCTG TCAGTCCCAC ATATTGTTTT
    GAACCGCCTC ATTATGATGG TATTGAAGCT CTAATCATCA GTGGAGATAA CTTGTTTAGT
    GGATCCCGAG ACAATGGAAT TAAGAAATGG GATTTATCAC GCAAAGACCT TCTACAGCAA
    GTACCAAACG CACACAAAGA CTGGGTGTGT GCCCTCGGTT TAGTGCCTGG TTGCCCTGTC
    CTTTTGAGCG GCTGTCGTGG AGGCATTCTG AAACTTTGGA ATGTGGACAC ATTCTTTGCT
    ATTGGAGAAA TCAAAGGACA TGATAGTCCC ATTAATGCAA TTTGTACAAA TTCGAGTCAC
    ATTTTCACAG CATCAGATGA TCAGACTGTA CGAATTTGGA AGGCTCATGG TTCATTGGAT
    GGACAGATGT CGGATACTGG CGATACGAGT GAAGATGTAG CCAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33419
    Clone ID Related Accession (Same CDS sequence) XM_012958731.2 , XM_012958731.3
    Accession Version XM_012958731.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814185.1
    CDS294..5183
    Translation

    Target ORF information:

    RefSeq Version XM_012958731.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958731.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGAAAATGC AGAAAACAGC AGTGATGATG ATGGATCATT ACAGAGTCCC
    AGTACAGAAG GCGGTTCCTT GACCTCTGAT CTCATGAAAC TGTGTGGTGA AGTCAAGCCA
    AAAAGCAAGG CACGGAGAAG AACCACAACC CAGCTTGAGT TACTCTATGC AGACAGCAGC
    GAGTCTACTC CAGATGCTGG CACTGGAGAG ACATGTATAC CAACTTCCCT CCCCCCTGTT
    TCAGAAATGC AAGTGTCTGG GCTGAATATA GAATCAAGTG TCAATACTGT TAAGGAGAAA
    GAGATGCTGC ATTCTGGCAT ACCTTCTAGA ACAGGCAGCA TATCAAAACC GGTAACTTTG
    ACTGACAAAA GAATTCCAGA ACAATCACCA GCAACAAGAA GGAGAGTATA TGACAAAGGT
    GGTGATAAAT CAATAAAAAA CAAAGAACAA AAGTTCTCAG ATTCTGGAAC CTCGGAGGCT
    AGTTTGTCTC CTCCTTCTTC CCCAACAAGC CGGCCCCGTA ATGAAAAGAA TGTTTTTAGC
    CGTCTCACAG TTTCTCAGGC AGACACAGCA TTTCAGCAGG ATAAGGGCGT TATTAACCCA
    TTTCCTGCAG CTAAAAGCAG TAAGACATCA CTTCTACAAT GCATTCATAT AGCTGAAGGG
    CACAACAAGG CTGTGCTGTG TGTGGACGCT ACTGATGATC TATTATTCAC TGGTTCCAAA
    GATCGTACTT GTAAAGTATG GAATCTTGTT ACTGGGCAAG AAATTATGTC CCTTGGAGGC
    CACCCCAATA ATGTTGTGTC TGTAAAGTAT TGTAACTACA CAAGCCTAGT GTTTACTGTT
    TCCACATCTT ACATTAAGGT GTGGGACATC AGAGACTCTG CTAAATGTAT TCGAACACTT
    ACGTCATCTG GGCAAGTCAT GACTTGGGAT TCTTGTGCTG GAAGTCAAAG CAGGACCGTA
    ACCATTCCAC CGGGTGAAAA TCAAATCAAT CAAATTGCAC TAAACCCAAC AGGCACTTTC
    TTATATGCAG CAGCTGGAAA TTCTGTGAGA ATGTGGGACC TTAAAAGGTT TCAATCAACA
    GGGAAATTAA CTGGACATCT TGGGCCCGTT ATGTGTCTTA CTGTGGATCA GAGTGCCAAT
    GGGCAAGACC TAATTATTTC AGGCTCAAAG GACCATTACA TTAAGATGTT TGACATTACA
    GAAGGGGCTT TGGGTTCTGT CAGTCCCACA TATTGTTTTG AACCGCCTCA TTATGATGGT
    ATTGAAGCTC TAATCATCAG TGGAGATAAC TTGTTTAGTG GATCCCGAGA CAATGGAATT
    AAGAAATGGG ATTTATCACG CAAAGACCTT CTACAGCAAG TACCAAACGC ACACAAAGAC
    TGGGTGTGTG CCCTCGGTTT AGTGCCTGGT TGCCCTGTCC TTTTGAGCGG CTGTCGTGGA
    GGCATTCTGA AACTTTGGAA TGTGGACACA TTCTTTGCTA TTGGAGAAAT CAAAGGACAT
    GATAGTCCCA TTAATGCAAT TTGTACAAAT TCGAGTCACA TTTTCACAGC ATCAGATGAT
    CAGACTGTAC GAATTTGGAA GGCTCATGGT TCATTGGATG GACAGATGTC GGATACTGGC
    GATACGAGTG AAGATGTAGC CAGCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33420
    Clone ID Related Accession (Same CDS sequence) XM_012958732.2 , XM_012958732.3
    Accession Version XM_012958732.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product kinesin-like protein KIF21A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC
    GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT
    ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC
    ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG
    TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA
    GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT
    ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA
    AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA
    ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG
    TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT
    CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG
    TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT
    CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA
    GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC
    GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC
    GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA
    ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA
    GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG
    TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814186.1
    CDS295..5076
    Translation

    Target ORF information:

    RefSeq Version XM_012958732.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis kinesin family member 21A (kif21a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012958732.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACGGGCG CTCAGGACGA CAGCGCGGTT AGGGTAGCCC TCCGAATTCG GCCACAACTA 
    GCAAAAGAAA AAATTGAAGG CTGCCATATT TGCACATCTG TTACCCCCGG GGAGCCTCAA
    GTAGTCCTAG GAAAAGACAA GGCTTTTACT TTTGACTATG TCTTCGATAT TGATTCAAGG
    CAGGAGGAAA TCTATATGCA GTGCACTGAA AAGCTTATTG AAGGCTGTTT TGAAGGATAC
    AATGCCACTG TTTTTGCTTA TGGCCAAACA GGTGCAGGCA AAACCTACAC AATGGGAACA
    GGATTTGATG TTAACATATC TGAGGAAGAG CATGGTATAA TTCCCCGTGC TGTTACACAT
    CTTTTTCGGC GCATCGAGGA AAAGAAACAG TTTGCATTGG AACAAGGCCT CCCACCCCCT
    GATTTTAAAG TTAATGCTCA GTTCCTGGAG CTTTACAATG AAGAAGTTCT TGATTTGTTT
    GATACTACAA GGGATATTGA TGCACGAAAT AAAAAATCCA ACATCAGAAT ACATGAAGAT
    TCATCTGGGG GAATTTATAC AGTTGGAGTC ACAACACGAA ATGTCAGCTC AGAAACAGAG
    ATGATACAGT GCTTGAAGAT TGGTGCCTTA TCTAGAACTA CAGCAAGTAC ACAGATGAAT
    GTTCAGAGTT CCCGTTCGCA TGCAATTTTC ACCATTCATT TATGTCAAAA TCGAGTTTGC
    CCTAAAATAG ACAATGAGAA TGATTTGGAT AACAGGATGG CCTCAGAATC TAATCAAATA
    AATGAATTTG AAACACTAAC TGCAAAATTC CATTTTGTTG ATTTGGCTGG CTCTGAACGT
    TTAAAGCGCA CAGGTGCTAC AGGAGAGAGG GCCAAGGAAG GCATTTCAAT TAACTGTGGG
    CTGCTGGCCC TGGGAAATGT TATAAGTGCT TTAGGAGACA AGAGTAAAAA GGCCACTCAC
    GTGCCTTATA GAGATTCCAA GTTAACACGG CTGCTGCAGG ACTCACTTGG TGGAAACAGC
    CAAACTGTTA TGATAGCTTG TGTAAGTCCT TCAGACAGAG ATTTCATGGA AACCTTAAAC
    ACACTTAAAT ATGCAAATCG GGCACGTAAT ATAAAAAATA AAGTGATGGT TAATCAAGAC
    CGAACAAGTC AACAGATAAA TGCACTGCGC AATGAGATTG CACGGCTTCA GATGGAGCTC
    ATGGAGTACA AAACGGGTAA AAGGATTATT GACGAGGAGG GAGTTGAGAG CATTAACGAC
    ATGTTTCACG AGAATGCAAT GCTACAGACA GAAAACAACA ATCTGCGTGT GAGAATTAAA
    GCAATGCAAG AAACTATTGA TGCTCTGAGA TCCAGGATAA CACAGCTCAT GAGTGATCAA
    GCCAATCAGG TCCTTTCTAG ATCAGGGGAT GGAAATGAAG AGATAAGCAA CATGATTCAC
    AATTATATTA AAGAGATTGA AGATCTCAGA GCAAAACTGT TAGAAAGTGA AGCAGTAAAT
    GAAAATCTGC GAAAGAGCTT ATCCAGAGCT TCTTCAAGGT CATCATACTT TCCTGCCCAT
    ACTCCTTTCC CCACATCTTT AATTGCTGCA GAACAAGAGA CCAATGAAAT AATAGATATG
    GCCAAAAAAG ATCTTGAAAA ACTGAAAAAA ATTGAGAAAA AGAAGAAGAA AAGCATTGTC
    AAAGAAGAGA ATCCTGATAA TGAGAAGTTA GAAGAAAAGG ACAATACAGA AAAGGAATGC
    CATGATTTGG TAATAGAGGA AAGGAGTGAC CATGAGGAAG ATGAAGAGGA AGAAGAGGAG
    GAAGAGGATG AAGATGTAGA TGCTGCTGAA AGTTCAGATG ATTCAGATTC TGAACTGGAG
    GAAAAAGAGA ATTACCAGGC TGATCTGGCA AATATTACTT GTGAAATTGC TATAAAGCAG
    AAGCTAATTG ATGAATTAGA AAACAGCCAG CGGAGACTGC ATACACTAAA ACAGCAGTAT
    GAAGAAAAAC TGATGACTCT GCAAAATAAA ATAAAGGACA CTCAACTAGA AAGAGATCGT
    GTTCTTCAAA ACATGGGATC AATGGAAACA TGCACAGAGG AAAAAGCAAA AAAAATTAAA
    GTGGAGTATG AAAAGAAACT GCAGACAATG AATAAGGAAC TTCAGAAACT TCAGGCTGCC
    CAAAAGGAAC ATGCTCGTTT GCTGAAAAAC CAGTCACAGT ATGAAAAACA AATGAAGAAA
    CTGCAGCAGG AGGTTGTAGA AATGAAGAAA ACAAAGGTTC GTCTAATGAA ACAGATGAAA
    GAAGAGCAAG AAAAAGCAAG AGTAACTGAA ACCCGCAGAA ACAGAGAAAT TGCACAGCTG
    AAAAAAGACC AGCGTAAACG AGATAGTCAG TTACGACTTT TAGAAGCCCA GAAAAAACAA
    CAAGAAGTTG TGCTGCGTCG TAAAACTGAA GAGGTTACAG CACTTCGTCG CCAAGTAAGA
    CCCATGTCTG ATAGGGTTGC AGGAAGGGTA GGCCGTAAAA TAAGTTTGCC TGAGCCTATT
    GCCGAACATA TTTCAAATAT GCCTGAGGAA CTGGATTCAA CCAAACATGG GTTACAGCAG
    AAACCAAGAA TTCCTGTTGC CAGAGTCCAG CCTGCCACGT CAACTGCCTT AAATGGGACA
    AGGAAGAAAT ATCAGAGAAA AGGAGTAAGT AGTTACACTT CAAAGGCAGC CAGACAGAAA
    TGGCAATCAC TGGAGCGCAG AATAACAGAC ATTGTGATGC AGAGAATGAC TATTTCCAAT
    ATGGAAGTTG ATATGAACAG ACTACTAAAG CAACGGGAGG AACTCACAAA AAGGAAGGAA
    AAGGTGTCAA AAAAAAGAGA TCGCATTTTA AAGGAATCTG GAGATGCAGA CAAAAATATT
    CATAGCCTCA GTGAAGAAAT GGAGTCCCTT AATGCAAATA TTGACTACCT TAATGATAGC
    ATATCTGACT GCCAAGCTAG CATTATGCAA ATGGAGGAGG CAAAGGAAGA AGGTGATACA
    CTGGATGTAA CAGCAGTGAT TAGTTCCTGC ACTTTAACTG AAGCACGTTA TCTTCTTGAT
    CATTTTCTGA ACATGGCTGT TAGTAAGAGT CTTCAAGCAG CACAAAGAGA GGCTCAAATT
    AAAGTTCTTG AAGGACGTCT TAAACAAACA GAAATAACAA GTGCAACCCA AAATCAGCTC
    TTGTTTCATA TGTTAAAAGA AAAAGCAGAA CTAAATCCAG AACTTGATGC GCTTCTTGGT
    CATGCCTTAC AAGATCTAGA TAGCATACCG CTAGAAAATG CAGAAAACAG CAGTGATGAT
    GATGGATCAT TACAGAGTCC CAGTACAGAA GGCGGTTCCT TGACCTCTGA TCTCATGAAA
    CTGTGTGGTG AAGTCAAGCC AAAAAGCAAG GCACGGAGAA GAACCACAAC CCAGCTTGAG
    TTACTCTATG CAGACAGCAG CGAGTCTACT CCAGATGCTG GCACTGGAGA GACATGTATA
    CCAACTTCCC TCCCCCCTGT TTCAGAAATG CAAGTGTCTG GGCTGAATAT AGAATCAAGT
    GTCAATACTG TTAAGGAGAA AGAGATGCTG CATTCTGGCA TACCTTCTAG AACAGGCAGC
    ATATCAAAAC CGGTAACTTT GACTGACAAA AGAATTCCAG AACAATCACC AGCAACAAGA
    AGGAGAGTAT ATGACAAAGG TGGTGATAAA TCAATAAAAA ACAAAGAACA AAAGTTGGGC
    GTTATTAACC CATTTCCTGC AGCTAAAAGC AGTAAGACAT CACTTCTACA ATGCATTCAT
    ATAGCTGAAG GGCACAACAA GGCTGTGCTG TGTGTGGACG CTACTGATGA TCTATTATTC
    ACTGGTTCCA AAGATCGTAC TTGTAAAGTA TGGAATCTTG TTACTGGGCA AGAAATTATG
    TCCCTTGGAG GCCACCCCAA TAATGTTGTG TCTGTAAAGT ATTGTAACTA CACAAGCCTA
    GTGTTTACTG TTTCCACATC TTACATTAAG GTGTGGGACA TCAGAGACTC TGCTAAATGT
    ATTCGAACAC TTACGTCATC TGGGCAAGTC ATGACTTGGG ATTCTTGTGC TGGAAGTCAA
    AGCAGGACCG TAACCATTCC ACCGGGTGAA AATCAAATCA ATCAAATTGC ACTAAACCCA
    ACAGGCACTT TCTTATATGC AGCAGCTGGA AATTCTGTGA GAATGTGGGA CCTTAAAAGG
    TTTCAATCAA CAGGGAAATT AACTGGACAT CTTGGGCCCG TTATGTGTCT TACTGTGGAT
    CAGAGTGCCA ATGGGCAAGA CCTAATTATT TCAGGCTCAA AGGACCATTA CATTAAGATG
    TTTGACATTA CAGAAGGGGC TTTGGGTTCT GTCAGTCCCA CATATTGTTT TGAACCGCCT
    CATTATGATG GTATTGAAGC TCTAATCATC AGTGGAGATA ACTTGTTTAG TGGATCCCGA
    GACAATGGAA TTAAGAAATG GGATTTATCA CGCAAAGACC TTCTACAGCA AGTACCAAAC
    GCACACAAAG ACTGGGTGTG TGCCCTCGGT TTAGTGCCTG GTTGCCCTGT CCTTTTGAGC
    GGCTGTCGTG GAGGCATTCT GAAACTTTGG AATGTGGACA CATTCTTTGC TATTGGAGAA
    ATCAAAGGAC ATGATAGTCC CATTAATGCA ATTTGTACAA ATTCGAGTCA CATTTTCACA
    GCATCAGATG ATCAGACTGT ACGAATTTGG AAGGCTCATG GTTCATTGGA TGGACAGATG
    TCGGATACTG GCGATACGAG TGAAGATGTA GCCAGCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.