Kif21a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif21a
Entrez Gene ID	16564
Full Name	kinesin family member 21A
General protein information	Preferred Names kinesin family member 21A Names kinesin-like protein KIF21A N-5 kinesin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 E3\|15 45.86 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316467.1	XP_017171956.1	kinesin-like protein KIF21A isoform X28
XM_017316454.1	XP_017171943.1	kinesin-like protein KIF21A isoform X15
XM_017316455.1	XP_017171944.1	kinesin-like protein KIF21A isoform X16
XM_017316457.1	XP_017171946.1	kinesin-like protein KIF21A isoform X18
XM_017316461.1	XP_017171950.1	kinesin-like protein KIF21A isoform X22
XM_017316462.1	XP_017171951.1	kinesin-like protein KIF21A isoform X23
XM_017316463.1	XP_017171952.1	kinesin-like protein KIF21A isoform X24
XM_017316466.1	XP_017171955.1	kinesin-like protein KIF21A isoform X27
XM_017316471.1	XP_017171960.1	kinesin-like protein KIF21A isoform X31
XM_017316472.1	XP_017171961.1	kinesin-like protein KIF21A isoform X32
XM_017316479.1	XP_017171968.1	kinesin-like protein KIF21A isoform X39
XM_017316450.2	XP_017171939.1	kinesin-like protein KIF21A isoform X11
XM_017316458.2	XP_017171947.1	kinesin-like protein KIF21A isoform X17
XM_017316475.2	XP_017171964.1	kinesin-like protein KIF21A isoform X29
XM_017316479.2	XP_017171968.1	kinesin-like protein KIF21A isoform X33
NM_016705.4	NP_057914.2	kinesin-like protein KIF21A isoform 3
NM_001358050.1	NP_001344979.1	kinesin-like protein KIF21A isoform 5
XM_017316444.1	XP_017171933.1	kinesin-like protein KIF21A isoform X4
XM_017316448.1	XP_017171937.1	kinesin-like protein KIF21A isoform X8
XM_006520495.3	XP_006520558.1	kinesin-like protein KIF21A isoform X9
XM_017316456.1	XP_017171945.1	kinesin-like protein KIF21A isoform X17
XM_017316460.1	XP_017171949.1	kinesin-like protein KIF21A isoform X21
XM_017316465.1	XP_017171954.1	kinesin-like protein KIF21A isoform X26
XM_017316448.2	XP_017171937.1	kinesin-like protein KIF21A isoform X8
XM_017316461.2	XP_017171950.1	kinesin-like protein KIF21A isoform X19
XM_017316462.2	XP_017171951.1	kinesin-like protein KIF21A isoform X21
XM_017316474.2	XP_017171963.1	kinesin-like protein KIF21A isoform X28
NM_001109042.2	NP_001102512.1	kinesin-like protein KIF21A isoform 4
XM_017316443.1	XP_017171932.1	kinesin-like protein KIF21A isoform X3
XM_017316451.1	XP_017171940.1	kinesin-like protein KIF21A isoform X12
XM_017316452.1	XP_017171941.1	kinesin-like protein KIF21A isoform X13
XM_017316453.1	XP_017171942.1	kinesin-like protein KIF21A isoform X14
XM_017316476.1	XP_017171965.1	kinesin-like protein KIF21A isoform X36
XM_017316446.2	XP_017171935.1	kinesin-like protein KIF21A isoform X6
XM_006520495.4	XP_006520558.1	kinesin-like protein KIF21A isoform X9
XM_017316453.2	XP_017171942.1	kinesin-like protein KIF21A isoform X13
XM_017316463.2	XP_017171952.1	kinesin-like protein KIF21A isoform X22
XM_017316465.2	XP_017171954.1	kinesin-like protein KIF21A isoform X24
XM_017316471.2	XP_017171960.1	kinesin-like protein KIF21A isoform X27
XM_017316477.2	XP_017171966.1	kinesin-like protein KIF21A isoform X31
XM_017316441.1	XP_017171930.1	kinesin-like protein KIF21A isoform X1
XM_017316442.1	XP_017171931.1	kinesin-like protein KIF21A isoform X2
XM_017316445.1	XP_017171934.1	kinesin-like protein KIF21A isoform X5
XM_017316446.1	XP_017171935.1	kinesin-like protein KIF21A isoform X6
XM_017316449.1	XP_017171938.1	kinesin-like protein KIF21A isoform X10
XM_017316458.1	XP_017171947.1	kinesin-like protein KIF21A isoform X19
XM_017316459.1	XP_017171948.1	kinesin-like protein KIF21A isoform X20
XM_017316464.1	XP_017171953.1	kinesin-like protein KIF21A isoform X25
XM_017316468.1	XP_017171957.1	kinesin-like protein KIF21A isoform X29
XM_017316473.1	XP_017171962.1	kinesin-like protein KIF21A isoform X33
XM_017316474.1	XP_017171963.1	kinesin-like protein KIF21A isoform X34
XM_017316475.1	XP_017171964.1	kinesin-like protein KIF21A isoform X35
XM_017316443.2	XP_017171932.1	kinesin-like protein KIF21A isoform X3
XM_017316447.2	XP_017171936.1	kinesin-like protein KIF21A isoform X7
XM_017316454.2	XP_017171943.1	kinesin-like protein KIF21A isoform X14
XM_017316464.2	XP_017171953.1	kinesin-like protein KIF21A isoform X23
XM_017316466.2	XP_017171955.1	kinesin-like protein KIF21A isoform X25
XM_017316476.2	XP_017171965.1	kinesin-like protein KIF21A isoform X30
NM_001109041.2	NP_001102511.1	kinesin-like protein KIF21A isoform 2
NM_001109040.2	NP_001102510.1	kinesin-like protein KIF21A isoform 1
XM_017316447.1	XP_017171936.1	kinesin-like protein KIF21A isoform X7
XM_017316450.1	XP_017171939.1	kinesin-like protein KIF21A isoform X11
XM_017316470.1	XP_017171959.1	kinesin-like protein KIF21A isoform X30
XM_017316477.1	XP_017171966.1	kinesin-like protein KIF21A isoform X37
XM_017316478.1	XP_017171967.1	kinesin-like protein KIF21A isoform X38
XM_017316441.2	XP_017171930.1	kinesin-like protein KIF21A isoform X1
XM_017316442.2	XP_017171931.1	kinesin-like protein KIF21A isoform X2
XM_017316444.2	XP_017171933.1	kinesin-like protein KIF21A isoform X4
XM_017316445.2	XP_017171934.1	kinesin-like protein KIF21A isoform X5
XM_017316449.2	XP_017171938.1	kinesin-like protein KIF21A isoform X10
XM_017316452.2	XP_017171941.1	kinesin-like protein KIF21A isoform X12
XM_017316455.2	XP_017171944.1	kinesin-like protein KIF21A isoform X15
XM_017316456.2	XP_017171945.1	kinesin-like protein KIF21A isoform X16
XM_017316460.2	XP_017171949.1	kinesin-like protein KIF21A isoform X18
XM_030248345.1	XP_030104205.1	kinesin-like protein KIF21A isoform X20
XM_017316468.2	XP_017171957.1	kinesin-like protein KIF21A isoform X26
XM_017316478.2	XP_017171967.1	kinesin-like protein KIF21A isoform X32

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	kif21a	XP_001920258.4
Canis lupus familiaris (dog)	KIF21A	XP_003433563.1
Mus musculus (house mouse)	Kif21a	NP_001102511.1
Gallus gallus (chicken)	KIF21A	XP_004934816.1
Drosophila melanogaster (fruit fly)	Klp31E	NP_001162937.2
Rattus norvegicus (Norway rat)	Kif21a	XP_006242318.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007815	XP_317685.4
Caenorhabditis elegans (roundworm)	klp-12	NP_001023349.1
Homo sapiens (human)	KIF21A	NP_001166935.1
	KIF21A	XP_002798575.1
Bos taurus (cattle)	KIF21A	NP_001193847.1
Xenopus tropicalis (tropical clawed frog)	kif21a	NP_001182593.1
Pan troglodytes (chimpanzee)	KIF21A	XP_003313814.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural analyses of key features in the KANK1?KIF21A complex yield mechanistic insights into the cross-talk between microtubules and the cell cortex.
Weng Z, Shang Y, Yao D, Zhu J, Zhang R
The Journal of biological chemistry293(1)215-225(2018 Jan)

Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex.
Zhang X, Chen MH, Wu X, Kodani A, Fan J, Doan R, Ozawa M, Ma J, Yoshida N, Reiter JF, Black DL, Kharchenko PV, Sharp PA, Walsh CA
Cell166(5)1147-1162.e15(2016 Aug)

Human CFEOM1 mutations attenuate KIF21A autoinhibition and cause oculomotor axon stalling.
Cheng L, Desai J, Miranda CJ, Duncan JS, Qiu W, Nugent AA, Kolpak AL, Wu CC, Drokhlyansky E, Delisle MM, Chan WM, Wei Y, Propst F, Reck-Peterson SL, Fritzsch B, Engle EC
Neuron82(2)334-49(2014 Apr)

Spatiotemporal expression pattern of KIF21A during normal embryonic development and in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).
Desai J, Velo MP, Yamada K, Overman LM, Engle EC
Gene expression patterns : GEP12(5-6)180-8(2012 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The diffuse and widespread expression of KIF21A in the developing human and mouse central and peripheral nervous system as well as in extraocular muscle does not account for the restricted ocular phenotype observed in Congenital fibrosis of the extraocular muscles type 1.
Title: Spatiotemporal expression pattern of KIF21A during normal embryonic development and in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).

The following Kif21a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif21a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04341	NM_001109042.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OMu50979	XM_006520495.4 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79007	XM_017316441.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79008	XM_017316442.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79009	XM_017316443.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79010	XM_017316444.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79011	XM_017316445.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79012	XM_017316446.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79013	XM_017316447.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79014	XM_017316448.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu79015	XM_017316449.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79016	XM_017316450.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79018	XM_017316452.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79019	XM_017316453.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79020	XM_017316454.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79021	XM_017316455.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79022	XM_017316456.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79024	XM_017316458.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79026	XM_017316460.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79027	XM_017316461.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79028	XM_017316462.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79029	XM_017316463.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79030	XM_017316464.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79031	XM_017316465.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79032	XM_017316466.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79034	XM_017316468.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79036	XM_017316471.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79039	XM_017316474.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79040	XM_017316475.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79041	XM_017316476.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79042	XM_017316477.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79043	XM_017316478.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79044	XM_017316479.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86647	XM_030248345.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu04339	NM_001109041.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$958.30 $1369.00
OMu10411	NM_016705.4 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OMu14016	NM_001109040.2 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1231.30 $1759.00
OMu79033	NM_001358050.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79017	XM_017316451.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79023	XM_017316457.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79025	XM_017316459.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79033	XM_017316467.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79035	XM_017316470.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79037	XM_017316472.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79038	XM_017316473.1 Latest version!	Mus musculus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OMu79044	XM_017316479.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79014	XM_017316448.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79027	XM_017316461.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79028	XM_017316462.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu50979	XM_006520495.3	Mus musculus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79019	XM_017316453.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79029	XM_017316463.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79031	XM_017316465.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79036	XM_017316471.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79012	XM_017316446.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79024	XM_017316458.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79039	XM_017316474.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79040	XM_017316475.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79009	XM_017316443.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79020	XM_017316454.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79030	XM_017316464.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79032	XM_017316466.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79041	XM_017316476.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79013	XM_017316447.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79016	XM_017316450.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79042	XM_017316477.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79007	XM_017316441.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79008	XM_017316442.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79010	XM_017316444.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79011	XM_017316445.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu79015	XM_017316449.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79018	XM_017316452.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79021	XM_017316455.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79022	XM_017316456.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79026	XM_017316460.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79034	XM_017316468.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu79043	XM_017316478.1	Mus musculus kinesin family member 21A (Kif21a), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif21a ( NM_001109042.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001102512.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant 4, mRNA.

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CloneID	OMu04341
Clone ID Related Accession (Same CDS sequence)	NM_001109042.2
Accession Version	NM_001109042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK019525.1, BY714299.1, BC060698.1, AK053373.1, AK052821.1 and AC136003.10. On Oct 23, 2013 this sequence version replaced NM_001109042.1. Transcript Variant: This variant (4) lacks four alternate exons in the central coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102512.1
CDS	250..4953
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	NM_001109042.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109042.2

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_006520495.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520558.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu50979
Clone ID Related Accession (Same CDS sequence)	XM_006520495.3 , XM_006520495.4
Accession Version	XM_006520495.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520495.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACACTCA GATTCGGGCG CTTCCGAGAC TAGTCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACT 
GTGCCTCAGG GAACCCCGTC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CTCCTCTTTG 
TCCGAGGTGC ACAGCAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520558.1
CDS	292..5286
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_006520495.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520495.4

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACACTCA GATTCGGGCG CTTCCGAGAC TAGTCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACT 
GTGCCTCAGG GAACCCCGTC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CTCCTCTTTG 
TCCGAGGTGC ACAGCAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316441.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171930.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

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CloneID	OMu79007
Clone ID Related Accession (Same CDS sequence)	XM_017316441.1 , XM_017316441.2
Accession Version	XM_017316441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGCAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171930.1
CDS	9004..14061
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316441.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316441.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGCAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316442.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171931.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

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CloneID	OMu79008
Clone ID Related Accession (Same CDS sequence)	XM_017316442.1 , XM_017316442.2
Accession Version	XM_017316442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171931.1
CDS	9004..14058
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316442.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316442.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316443.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171932.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

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CloneID	OMu79009
Clone ID Related Accession (Same CDS sequence)	XM_017316443.1 , XM_017316443.2
Accession Version	XM_017316443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171932.1
CDS	9004..14046
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316443.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316443.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316444.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171933.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

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CloneID	OMu79010
Clone ID Related Accession (Same CDS sequence)	XM_017316444.1 , XM_017316444.2
Accession Version	XM_017316444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACACT CAGATTCGGG CGCTTCCGAG ACTAGTCTCT CACCTCCTTC CTCCCCACCA 
AGCCGGCCCC GTAACGAACT GAATGTGTTT AATCGCCTTA CTGTGCCTCA GGGAACCCCG 
TCAGTTCAGC AGGATAAGTC TGATGAAAGT GACTCCTCTT TGTCCGAGGT GCACAGCAGA 
TCCACCAGAA GAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171933.1
CDS	9004..14040
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316444.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316444.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACACT CAGATTCGGG CGCTTCCGAG ACTAGTCTCT CACCTCCTTC CTCCCCACCA 
AGCCGGCCCC GTAACGAACT GAATGTGTTT AATCGCCTTA CTGTGCCTCA GGGAACCCCG 
TCAGTTCAGC AGGATAAGTC TGATGAAAGT GACTCCTCTT TGTCCGAGGT GCACAGCAGA 
TCCACCAGAA GAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316445.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171934.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

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CloneID	OMu79011
Clone ID Related Accession (Same CDS sequence)	XM_017316445.1 , XM_017316445.2
Accession Version	XM_017316445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGGCTACAG 
AAACTTGAGG AAAGCGGTCG AGAAGAAAGA AGTGTTGCCG GGAAAGACGA TAATGCAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT GTTTCAGAGA AAGAAAACAA TGAGCTAGAC 
GTGGAAGAGA ATCAAGAAGT GAGTGACCAC GAGGATGAGG AAGAGGAGGA AGAGGACGAG 
GAGGAAGAGG ATGACATTGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCTGAT 
GAAAAAGCTA ACTATCAAGC CGACTTAGCA AATATCACCT GCGAGATTGC GATTAAGCAG 
AAGCTGATCG ACGAACTGGA GAACAGCCAG AAACGGCTGC AGACCCTGAA AAAGCAGTAC 
GAGGAGAAGC TGATGATGCT CCAACATAAG ATCCGGGACA CGCAGCTGGA GAGGGACCAG 
GTGCTCCAGA ACCTAGGCTC GGTGGAGTCA TACTCGGAAG AAAAGGCCAA GAAAGTGAAG 
TGCGAATATG AGAAGAAGCT CCACGCCATG AACAAAGAGC TGCAGCGACT GCAGACGGCC 
CAAAAGGAGC ACGCCAGGCT CCTCAAAAAC CAGTCTCAGT ATGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCATGGA AATGAAGAAA ACCAAGGTTC GTCTAATGAA GCAGATGAAA 
GAAGAGCAGG AGAAAGCCCG GCTGACAGAA TCTCGAAGGA ACCGGGAAAT CGCTCAGCTG 
AAGAAGGATC AGCGCAAGCG AGATCATCAA CTTAGACTTC TAGAGGCCCA GAAAAGAAAT 
CAAGAAGTAG TTCTGCGACG CAAAACGGAA GAGGTTACAG CTCTCCGCAG GCAAGTGAGG 
CCCATGTCTG ATAAAGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAAAGCCCC 
GCTCCGGACA CAGGTTCCAG TGCGGCTTCC GGGGAAGCAG ACACATCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATCCC CGTGGCAAGA GTCCAGGCAT TACCAACACC TACAACAAAT 
GGCACCAGGA AAAAATATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACA 
GCCCGCATGA AGTGGCAGCT ACTGGAGCGC CGGGTGACCG ACATCATCAT GCAGAAAATG 
ACCATCTCCA ACATGGAGGC GGACATGAAC AGACTCCTCA GGCAACGGGA AGAACTCACA 
AAAAGGCGAG AGAAACTTTC TAAAAGGAGA GAGAAGATAG TCAAGGAGAG CGGAGAGGGA 
GATAAAAGTG TGGCTAACAT CATCGAGGAG ATGGAGTCCC TGACAGCCAA CATAGATTAC 
ATCAATGATA GCATTGCCGA CTGTCAAGCC AACATCATGC AGATGGAGGA GGCAAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATTAATGCCT GTACACTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTGCAGGC TGCCCAGAAA 
GAGGCTCAAA TTAAAGTCCT CGAGGGTCGA CTCAAACAGA CCGAAATCAC CAGTGCAACC 
CAGAACCAAC TCTTATTCCA TATGCTGAAG GAGAAGGCAG AGCTAAACCC AGAGCTGGAT 
GCCTTGCTAG GCCACGCACT GCAAGATCTA GATGGCGCCC CACCAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATTTC CAGACAGTCA TCTCTATCAG AAAAAAAAGT GCCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAGGCAGATA AACCAAAGGC GAAGGAACAT 
AAACACTCAG ATTCGGGCGC TTCCGAGACT AGTCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACTG TGCCTCAGGG AACCCCGTCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC TCCTCTTTGT CCGAGGTGCA CAGCAGATCC 
ACCAGAAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171934.1
CDS	292..5325
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316445.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316445.2

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGGCTACAG 
AAACTTGAGG AAAGCGGTCG AGAAGAAAGA AGTGTTGCCG GGAAAGACGA TAATGCAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT GTTTCAGAGA AAGAAAACAA TGAGCTAGAC 
GTGGAAGAGA ATCAAGAAGT GAGTGACCAC GAGGATGAGG AAGAGGAGGA AGAGGACGAG 
GAGGAAGAGG ATGACATTGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCTGAT 
GAAAAAGCTA ACTATCAAGC CGACTTAGCA AATATCACCT GCGAGATTGC GATTAAGCAG 
AAGCTGATCG ACGAACTGGA GAACAGCCAG AAACGGCTGC AGACCCTGAA AAAGCAGTAC 
GAGGAGAAGC TGATGATGCT CCAACATAAG ATCCGGGACA CGCAGCTGGA GAGGGACCAG 
GTGCTCCAGA ACCTAGGCTC GGTGGAGTCA TACTCGGAAG AAAAGGCCAA GAAAGTGAAG 
TGCGAATATG AGAAGAAGCT CCACGCCATG AACAAAGAGC TGCAGCGACT GCAGACGGCC 
CAAAAGGAGC ACGCCAGGCT CCTCAAAAAC CAGTCTCAGT ATGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCATGGA AATGAAGAAA ACCAAGGTTC GTCTAATGAA GCAGATGAAA 
GAAGAGCAGG AGAAAGCCCG GCTGACAGAA TCTCGAAGGA ACCGGGAAAT CGCTCAGCTG 
AAGAAGGATC AGCGCAAGCG AGATCATCAA CTTAGACTTC TAGAGGCCCA GAAAAGAAAT 
CAAGAAGTAG TTCTGCGACG CAAAACGGAA GAGGTTACAG CTCTCCGCAG GCAAGTGAGG 
CCCATGTCTG ATAAAGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAAAGCCCC 
GCTCCGGACA CAGGTTCCAG TGCGGCTTCC GGGGAAGCAG ACACATCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATCCC CGTGGCAAGA GTCCAGGCAT TACCAACACC TACAACAAAT 
GGCACCAGGA AAAAATATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACA 
GCCCGCATGA AGTGGCAGCT ACTGGAGCGC CGGGTGACCG ACATCATCAT GCAGAAAATG 
ACCATCTCCA ACATGGAGGC GGACATGAAC AGACTCCTCA GGCAACGGGA AGAACTCACA 
AAAAGGCGAG AGAAACTTTC TAAAAGGAGA GAGAAGATAG TCAAGGAGAG CGGAGAGGGA 
GATAAAAGTG TGGCTAACAT CATCGAGGAG ATGGAGTCCC TGACAGCCAA CATAGATTAC 
ATCAATGATA GCATTGCCGA CTGTCAAGCC AACATCATGC AGATGGAGGA GGCAAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATTAATGCCT GTACACTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTGCAGGC TGCCCAGAAA 
GAGGCTCAAA TTAAAGTCCT CGAGGGTCGA CTCAAACAGA CCGAAATCAC CAGTGCAACC 
CAGAACCAAC TCTTATTCCA TATGCTGAAG GAGAAGGCAG AGCTAAACCC AGAGCTGGAT 
GCCTTGCTAG GCCACGCACT GCAAGATCTA GATGGCGCCC CACCAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATTTC CAGACAGTCA TCTCTATCAG AAAAAAAAGT GCCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAGGCAGATA AACCAAAGGC GAAGGAACAT 
AAACACTCAG ATTCGGGCGC TTCCGAGACT AGTCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACTG TGCCTCAGGG AACCCCGTCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC TCCTCTTTGT CCGAGGTGCA CAGCAGATCC 
ACCAGAAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316446.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171935.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu79012
Clone ID Related Accession (Same CDS sequence)	XM_017316446.2 , XM_017316446.1
Accession Version	XM_017316446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGCA GATCCACCAG AAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171935.1
CDS	9004..14022
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316446.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316446.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGCA GATCCACCAG AAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316447.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171936.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu79013
Clone ID Related Accession (Same CDS sequence)	XM_017316447.2 , XM_017316447.1
Accession Version	XM_017316447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGAT CCACCAGAAG AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171936.1
CDS	9004..14019
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316447.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316447.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGAT CCACCAGAAG AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316448.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171937.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.

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CloneID	OMu79014
Clone ID Related Accession (Same CDS sequence)	XM_017316448.1 , XM_017316448.2
Accession Version	XM_017316448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171937.1
CDS	9004..14007
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316448.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316448.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316449.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171938.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.

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CloneID	OMu79015
Clone ID Related Accession (Same CDS sequence)	XM_017316449.1 , XM_017316449.2
Accession Version	XM_017316449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171938.1
CDS	9004..13968
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316449.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316449.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316450.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171939.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.

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CloneID	OMu79016
Clone ID Related Accession (Same CDS sequence)	XM_017316450.2 , XM_017316450.1
Accession Version	XM_017316450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TCTCAGATTC GGGCGCTTCC 
GAGACTAGTC TCTCACCTCC TTCCTCCCCA CCAAGCCGGC CCCGTAACGA ACTGAATGTG 
TTTAATCGCC TTACTGTGCC TCAGGGAACC CCGTCAGTTC AGCAGGATAA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGC AGATCCACCA GAAGAGGCAT AATCAACCCA 
TTTCCTGCCT GCAAAGGAGT CAGAGCCTCT CCTCTTCAGT GCGTTCACAT AGCTGAAGGG 
CACACCAAGG CTGTGCTCTG TGTGGATTCT ACGGATGATC TTCTCTTCAC CGGATCAAAA 
GATCGCACTT GTAAAGTATG GAATCTCGTG ACTGGACAGG AAATTATGTC CCTGGGGGTT 
CACCCTAACA ACGTGGTGTC TGTAAAATAC TGTAATTATA CCAGTCTGGT CTTCACCGTG 
TCGACATCTT ATATTAAGGT GTGGGATATC AGAGAGTCAG CAAAATGCAT TCGAACATTA 
ACATCTTCAG GTCAAGTTAC CCTTGGAGAA GCGTGTTCTG CCAGCACCAG CCGGACAGTA 
GCTATTCCTT CCGGGGAGAG CCAGATCAAT CAAATTGCAC TAAACCCAAC TGGCACTTTC 
CTCTACGCGG CCTCTGGAAA TGCCGTCAGA ATGTGGGACC TTAAAAGGTT TCAGTCTACA 
GGAAAGCTAA CCGGACACCT GGGTCCTGTT ATGTGCCTTA CGGTAGACCA GATCTCCAAT 
GGACAGGACC TCATCATCAC TGGCTCAAAA GACCACTACA TCAAAATGTT CGATGTGACT 
GAAGGGGCTC TTGGAACTGT AAGTCCCACC CACAACTTCG AGCCTCCTCA TTATGATGGG 
ATAGAAGCAC TGGCCATTCA AGGCGATAAC CTATTCAGTG GGTCCAGAGA TAATGGAATC 
AAGAAATGGG ACTTAGCTCA GAAAGGTCTT CTTCAGCAAG TCCCAAATGC ACACAAAGAC 
TGGGTGTGTG CCCTGGGCCT GGTGCCAGGC CATCCGGTTT TGCTGAGTGG CTGCAGAGGC 
GGCATTCTGA AACTCTGGAA TGTGGACACT TTTGTGCCCG TTGGAGAGAT GAGAGGTCAT 
GACAGTCCCA TCAATGCCAT TTGTGTTAAC TCCACCCATG TCTTTACTGC TGCTGATGAT 
CGAACCGTGA GAATCTGGAA GGCCCACAAC TTGCAAGATG GTCAACTCTC TGACACCGGC 
GATCTGGGGG AGGATATTGC CAGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171939.1
CDS	9004..13953
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316450.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316450.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TCTCAGATTC GGGCGCTTCC 
GAGACTAGTC TCTCACCTCC TTCCTCCCCA CCAAGCCGGC CCCGTAACGA ACTGAATGTG 
TTTAATCGCC TTACTGTGCC TCAGGGAACC CCGTCAGTTC AGCAGGATAA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGC AGATCCACCA GAAGAGGCAT AATCAACCCA 
TTTCCTGCCT GCAAAGGAGT CAGAGCCTCT CCTCTTCAGT GCGTTCACAT AGCTGAAGGG 
CACACCAAGG CTGTGCTCTG TGTGGATTCT ACGGATGATC TTCTCTTCAC CGGATCAAAA 
GATCGCACTT GTAAAGTATG GAATCTCGTG ACTGGACAGG AAATTATGTC CCTGGGGGTT 
CACCCTAACA ACGTGGTGTC TGTAAAATAC TGTAATTATA CCAGTCTGGT CTTCACCGTG 
TCGACATCTT ATATTAAGGT GTGGGATATC AGAGAGTCAG CAAAATGCAT TCGAACATTA 
ACATCTTCAG GTCAAGTTAC CCTTGGAGAA GCGTGTTCTG CCAGCACCAG CCGGACAGTA 
GCTATTCCTT CCGGGGAGAG CCAGATCAAT CAAATTGCAC TAAACCCAAC TGGCACTTTC 
CTCTACGCGG CCTCTGGAAA TGCCGTCAGA ATGTGGGACC TTAAAAGGTT TCAGTCTACA 
GGAAAGCTAA CCGGACACCT GGGTCCTGTT ATGTGCCTTA CGGTAGACCA GATCTCCAAT 
GGACAGGACC TCATCATCAC TGGCTCAAAA GACCACTACA TCAAAATGTT CGATGTGACT 
GAAGGGGCTC TTGGAACTGT AAGTCCCACC CACAACTTCG AGCCTCCTCA TTATGATGGG 
ATAGAAGCAC TGGCCATTCA AGGCGATAAC CTATTCAGTG GGTCCAGAGA TAATGGAATC 
AAGAAATGGG ACTTAGCTCA GAAAGGTCTT CTTCAGCAAG TCCCAAATGC ACACAAAGAC 
TGGGTGTGTG CCCTGGGCCT GGTGCCAGGC CATCCGGTTT TGCTGAGTGG CTGCAGAGGC 
GGCATTCTGA AACTCTGGAA TGTGGACACT TTTGTGCCCG TTGGAGAGAT GAGAGGTCAT 
GACAGTCCCA TCAATGCCAT TTGTGTTAAC TCCACCCATG TCTTTACTGC TGCTGATGAT 
CGAACCGTGA GAATCTGGAA GGCCCACAAC TTGCAAGATG GTCAACTCTC TGACACCGGC 
GATCTGGGGG AGGATATTGC CAGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316452.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171941.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.

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CloneID	OMu79018
Clone ID Related Accession (Same CDS sequence)	XM_017316452.1 , XM_017316452.2
Accession Version	XM_017316452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171941.1
CDS	9004..13947
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316452.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316452.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316453.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171942.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.

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CloneID	OMu79019
Clone ID Related Accession (Same CDS sequence)	XM_017316453.1 , XM_017316453.2
Accession Version	XM_017316453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGCA GATCCACCAG AAGAGGCATA ATCAACCCAT TTCCTGCCTG CAAAGGAGTC 
AGAGCCTCTC CTCTTCAGTG CGTTCACATA GCTGAAGGGC ACACCAAGGC TGTGCTCTGT 
GTGGATTCTA CGGATGATCT TCTCTTCACC GGATCAAAAG ATCGCACTTG TAAAGTATGG 
AATCTCGTGA CTGGACAGGA AATTATGTCC CTGGGGGTTC ACCCTAACAA CGTGGTGTCT 
GTAAAATACT GTAATTATAC CAGTCTGGTC TTCACCGTGT CGACATCTTA TATTAAGGTG 
TGGGATATCA GAGAGTCAGC AAAATGCATT CGAACATTAA CATCTTCAGG TCAAGTTACC 
CTTGGAGAAG CGTGTTCTGC CAGCACCAGC CGGACAGTAG CTATTCCTTC CGGGGAGAGC 
CAGATCAATC AAATTGCACT AAACCCAACT GGCACTTTCC TCTACGCGGC CTCTGGAAAT 
GCCGTCAGAA TGTGGGACCT TAAAAGGTTT CAGTCTACAG GAAAGCTAAC CGGACACCTG 
GGTCCTGTTA TGTGCCTTAC GGTAGACCAG ATCTCCAATG GACAGGACCT CATCATCACT 
GGCTCAAAAG ACCACTACAT CAAAATGTTC GATGTGACTG AAGGGGCTCT TGGAACTGTA 
AGTCCCACCC ACAACTTCGA GCCTCCTCAT TATGATGGGA TAGAAGCACT GGCCATTCAA 
GGCGATAACC TATTCAGTGG GTCCAGAGAT AATGGAATCA AGAAATGGGA CTTAGCTCAG 
AAAGGTCTTC TTCAGCAAGT CCCAAATGCA CACAAAGACT GGGTGTGTGC CCTGGGCCTG 
GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC TGCAGAGGCG GCATTCTGAA ACTCTGGAAT 
GTGGACACTT TTGTGCCCGT TGGAGAGATG AGAGGTCATG ACAGTCCCAT CAATGCCATT 
TGTGTTAACT CCACCCATGT CTTTACTGCT GCTGATGATC GAACCGTGAG AATCTGGAAG 
GCCCACAACT TGCAAGATGG TCAACTCTCT GACACCGGCG ATCTGGGGGA GGATATTGCC 
AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171942.1
CDS	9004..13932
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316453.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316453.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGCA GATCCACCAG AAGAGGCATA ATCAACCCAT TTCCTGCCTG CAAAGGAGTC 
AGAGCCTCTC CTCTTCAGTG CGTTCACATA GCTGAAGGGC ACACCAAGGC TGTGCTCTGT 
GTGGATTCTA CGGATGATCT TCTCTTCACC GGATCAAAAG ATCGCACTTG TAAAGTATGG 
AATCTCGTGA CTGGACAGGA AATTATGTCC CTGGGGGTTC ACCCTAACAA CGTGGTGTCT 
GTAAAATACT GTAATTATAC CAGTCTGGTC TTCACCGTGT CGACATCTTA TATTAAGGTG 
TGGGATATCA GAGAGTCAGC AAAATGCATT CGAACATTAA CATCTTCAGG TCAAGTTACC 
CTTGGAGAAG CGTGTTCTGC CAGCACCAGC CGGACAGTAG CTATTCCTTC CGGGGAGAGC 
CAGATCAATC AAATTGCACT AAACCCAACT GGCACTTTCC TCTACGCGGC CTCTGGAAAT 
GCCGTCAGAA TGTGGGACCT TAAAAGGTTT CAGTCTACAG GAAAGCTAAC CGGACACCTG 
GGTCCTGTTA TGTGCCTTAC GGTAGACCAG ATCTCCAATG GACAGGACCT CATCATCACT 
GGCTCAAAAG ACCACTACAT CAAAATGTTC GATGTGACTG AAGGGGCTCT TGGAACTGTA 
AGTCCCACCC ACAACTTCGA GCCTCCTCAT TATGATGGGA TAGAAGCACT GGCCATTCAA 
GGCGATAACC TATTCAGTGG GTCCAGAGAT AATGGAATCA AGAAATGGGA CTTAGCTCAG 
AAAGGTCTTC TTCAGCAAGT CCCAAATGCA CACAAAGACT GGGTGTGTGC CCTGGGCCTG 
GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC TGCAGAGGCG GCATTCTGAA ACTCTGGAAT 
GTGGACACTT TTGTGCCCGT TGGAGAGATG AGAGGTCATG ACAGTCCCAT CAATGCCATT 
TGTGTTAACT CCACCCATGT CTTTACTGCT GCTGATGATC GAACCGTGAG AATCTGGAAG 
GCCCACAACT TGCAAGATGG TCAACTCTCT GACACCGGCG ATCTGGGGGA GGATATTGCC 
AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316454.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171943.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu79020
Clone ID Related Accession (Same CDS sequence)	XM_017316454.1 , XM_017316454.2
Accession Version	XM_017316454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAT CCACCAGAAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171943.1
CDS	9004..13929
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316454.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316454.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAT CCACCAGAAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316455.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171944.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.

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CloneID	OMu79021
Clone ID Related Accession (Same CDS sequence)	XM_017316455.1 , XM_017316455.2
Accession Version	XM_017316455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171944.1
CDS	9004..13917
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316455.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316455.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316456.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171945.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.

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CloneID	OMu79022
Clone ID Related Accession (Same CDS sequence)	XM_017316456.1 , XM_017316456.2
Accession Version	XM_017316456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACTCC TCTTTGTCCG AGGTGCACAG CAGATCCACC 
AGAAGAGGCA TAATCAACCC ATTTCCTGCC TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG 
TGCGTTCACA TAGCTGAAGG GCACACCAAG GCTGTGCTCT GTGTGGATTC TACGGATGAT 
CTTCTCTTCA CCGGATCAAA AGATCGCACT TGTAAAGTAT GGAATCTCGT GACTGGACAG 
GAAATTATGT CCCTGGGGGT TCACCCTAAC AACGTGGTGT CTGTAAAATA CTGTAATTAT 
ACCAGTCTGG TCTTCACCGT GTCGACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCAAAATGCA TTCGAACATT AACATCTTCA GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT 
GCCAGCACCA GCCGGACAGT AGCTATTCCT TCCGGGGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACTTT CCTCTACGCG GCCTCTGGAA ATGCCGTCAG AATGTGGGAC 
CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA ACCGGACACC TGGGTCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA TGGACAGGAC CTCATCATCA CTGGCTCAAA AGACCACTAC 
ATCAAAATGT TCGATGTGAC TGAAGGGGCT CTTGGAACTG TAAGTCCCAC CCACAACTTC 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGCGATAA CCTATTCAGT 
GGGTCCAGAG ATAATGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGTCT TCTTCAGCAA 
GTCCCAAATG CACACAAAGA CTGGGTGTGT GCCCTGGGCC TGGTGCCAGG CCATCCGGTT 
TTGCTGAGTG GCTGCAGAGG CGGCATTCTG AAACTCTGGA ATGTGGACAC TTTTGTGCCC 
GTTGGAGAGA TGAGAGGTCA TGACAGTCCC ATCAATGCCA TTTGTGTTAA CTCCACCCAT 
GTCTTTACTG CTGCTGATGA TCGAACCGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAACTCT CTGACACCGG CGATCTGGGG GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171945.1
CDS	9004..13914
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316456.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316456.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACTCC TCTTTGTCCG AGGTGCACAG CAGATCCACC 
AGAAGAGGCA TAATCAACCC ATTTCCTGCC TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG 
TGCGTTCACA TAGCTGAAGG GCACACCAAG GCTGTGCTCT GTGTGGATTC TACGGATGAT 
CTTCTCTTCA CCGGATCAAA AGATCGCACT TGTAAAGTAT GGAATCTCGT GACTGGACAG 
GAAATTATGT CCCTGGGGGT TCACCCTAAC AACGTGGTGT CTGTAAAATA CTGTAATTAT 
ACCAGTCTGG TCTTCACCGT GTCGACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCAAAATGCA TTCGAACATT AACATCTTCA GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT 
GCCAGCACCA GCCGGACAGT AGCTATTCCT TCCGGGGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACTTT CCTCTACGCG GCCTCTGGAA ATGCCGTCAG AATGTGGGAC 
CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA ACCGGACACC TGGGTCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA TGGACAGGAC CTCATCATCA CTGGCTCAAA AGACCACTAC 
ATCAAAATGT TCGATGTGAC TGAAGGGGCT CTTGGAACTG TAAGTCCCAC CCACAACTTC 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGCGATAA CCTATTCAGT 
GGGTCCAGAG ATAATGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGTCT TCTTCAGCAA 
GTCCCAAATG CACACAAAGA CTGGGTGTGT GCCCTGGGCC TGGTGCCAGG CCATCCGGTT 
TTGCTGAGTG GCTGCAGAGG CGGCATTCTG AAACTCTGGA ATGTGGACAC TTTTGTGCCC 
GTTGGAGAGA TGAGAGGTCA TGACAGTCCC ATCAATGCCA TTTGTGTTAA CTCCACCCAT 
GTCTTTACTG CTGCTGATGA TCGAACCGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAACTCT CTGACACCGG CGATCTGGGG GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316458.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171947.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.

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CloneID	OMu79024
Clone ID Related Accession (Same CDS sequence)	XM_017316458.2 , XM_017316458.1
Accession Version	XM_017316458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAGT CTGATGAAAG TGACTCCTCT TTGTCCGAGG TGCACAGAGG CATAATCAAC 
CCATTTCCTG CCTGCAAAGG AGTCAGAGCC TCTCCTCTTC AGTGCGTTCA CATAGCTGAA 
GGGCACACCA AGGCTGTGCT CTGTGTGGAT TCTACGGATG ATCTTCTCTT CACCGGATCA 
AAAGATCGCA CTTGTAAAGT ATGGAATCTC GTGACTGGAC AGGAAATTAT GTCCCTGGGG 
GTTCACCCTA ACAACGTGGT GTCTGTAAAA TACTGTAATT ATACCAGTCT GGTCTTCACC 
GTGTCGACAT CTTATATTAA GGTGTGGGAT ATCAGAGAGT CAGCAAAATG CATTCGAACA 
TTAACATCTT CAGGTCAAGT TACCCTTGGA GAAGCGTGTT CTGCCAGCAC CAGCCGGACA 
GTAGCTATTC CTTCCGGGGA GAGCCAGATC AATCAAATTG CACTAAACCC AACTGGCACT 
TTCCTCTACG CGGCCTCTGG AAATGCCGTC AGAATGTGGG ACCTTAAAAG GTTTCAGTCT 
ACAGGAAAGC TAACCGGACA CCTGGGTCCT GTTATGTGCC TTACGGTAGA CCAGATCTCC 
AATGGACAGG ACCTCATCAT CACTGGCTCA AAAGACCACT ACATCAAAAT GTTCGATGTG 
ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC ACCCACAACT TCGAGCCTCC TCATTATGAT 
GGGATAGAAG CACTGGCCAT TCAAGGCGAT AACCTATTCA GTGGGTCCAG AGATAATGGA 
ATCAAGAAAT GGGACTTAGC TCAGAAAGGT CTTCTTCAGC AAGTCCCAAA TGCACACAAA 
GACTGGGTGT GTGCCCTGGG CCTGGTGCCA GGCCATCCGG TTTTGCTGAG TGGCTGCAGA 
GGCGGCATTC TGAAACTCTG GAATGTGGAC ACTTTTGTGC CCGTTGGAGA GATGAGAGGT 
CATGACAGTC CCATCAATGC CATTTGTGTT AACTCCACCC ATGTCTTTAC TGCTGCTGAT 
GATCGAACCG TGAGAATCTG GAAGGCCCAC AACTTGCAAG ATGGTCAACT CTCTGACACC 
GGCGATCTGG GGGAGGATAT TGCCAGTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171947.1
CDS	9004..13896
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316458.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316458.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAGT CTGATGAAAG TGACTCCTCT TTGTCCGAGG TGCACAGAGG CATAATCAAC 
CCATTTCCTG CCTGCAAAGG AGTCAGAGCC TCTCCTCTTC AGTGCGTTCA CATAGCTGAA 
GGGCACACCA AGGCTGTGCT CTGTGTGGAT TCTACGGATG ATCTTCTCTT CACCGGATCA 
AAAGATCGCA CTTGTAAAGT ATGGAATCTC GTGACTGGAC AGGAAATTAT GTCCCTGGGG 
GTTCACCCTA ACAACGTGGT GTCTGTAAAA TACTGTAATT ATACCAGTCT GGTCTTCACC 
GTGTCGACAT CTTATATTAA GGTGTGGGAT ATCAGAGAGT CAGCAAAATG CATTCGAACA 
TTAACATCTT CAGGTCAAGT TACCCTTGGA GAAGCGTGTT CTGCCAGCAC CAGCCGGACA 
GTAGCTATTC CTTCCGGGGA GAGCCAGATC AATCAAATTG CACTAAACCC AACTGGCACT 
TTCCTCTACG CGGCCTCTGG AAATGCCGTC AGAATGTGGG ACCTTAAAAG GTTTCAGTCT 
ACAGGAAAGC TAACCGGACA CCTGGGTCCT GTTATGTGCC TTACGGTAGA CCAGATCTCC 
AATGGACAGG ACCTCATCAT CACTGGCTCA AAAGACCACT ACATCAAAAT GTTCGATGTG 
ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC ACCCACAACT TCGAGCCTCC TCATTATGAT 
GGGATAGAAG CACTGGCCAT TCAAGGCGAT AACCTATTCA GTGGGTCCAG AGATAATGGA 
ATCAAGAAAT GGGACTTAGC TCAGAAAGGT CTTCTTCAGC AAGTCCCAAA TGCACACAAA 
GACTGGGTGT GTGCCCTGGG CCTGGTGCCA GGCCATCCGG TTTTGCTGAG TGGCTGCAGA 
GGCGGCATTC TGAAACTCTG GAATGTGGAC ACTTTTGTGC CCGTTGGAGA GATGAGAGGT 
CATGACAGTC CCATCAATGC CATTTGTGTT AACTCCACCC ATGTCTTTAC TGCTGCTGAT 
GATCGAACCG TGAGAATCTG GAAGGCCCAC AACTTGCAAG ATGGTCAACT CTCTGACACC 
GGCGATCTGG GGGAGGATAT TGCCAGTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316460.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171949.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.

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CloneID	OMu79026
Clone ID Related Accession (Same CDS sequence)	XM_017316460.1 , XM_017316460.2
Accession Version	XM_017316460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171949.1
CDS	9004..13878
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316460.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316460.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316461.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171950.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu79027
Clone ID Related Accession (Same CDS sequence)	XM_017316461.1 , XM_017316461.2
Accession Version	XM_017316461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171950.1
CDS	9004..13860
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316461.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316461.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316462.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171951.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu79028
Clone ID Related Accession (Same CDS sequence)	XM_017316462.1 , XM_017316462.2
Accession Version	XM_017316462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171951.1
CDS	9004..13857
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316462.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316462.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316463.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171952.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.

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CloneID	OMu79029
Clone ID Related Accession (Same CDS sequence)	XM_017316463.1 , XM_017316463.2
Accession Version	XM_017316463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171952.1
CDS	9004..13839
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316463.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316463.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316464.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171953.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.

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CloneID	OMu79030
Clone ID Related Accession (Same CDS sequence)	XM_017316464.1 , XM_017316464.2
Accession Version	XM_017316464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG CAGATCCACC AGAAGAGGCA TAATCAACCC ATTTCCTGCC 
TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG TGCGTTCACA TAGCTGAAGG GCACACCAAG 
GCTGTGCTCT GTGTGGATTC TACGGATGAT CTTCTCTTCA CCGGATCAAA AGATCGCACT 
TGTAAAGTAT GGAATCTCGT GACTGGACAG GAAATTATGT CCCTGGGGGT TCACCCTAAC 
AACGTGGTGT CTGTAAAATA CTGTAATTAT ACCAGTCTGG TCTTCACCGT GTCGACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCAAAATGCA TTCGAACATT AACATCTTCA 
GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT GCCAGCACCA GCCGGACAGT AGCTATTCCT 
TCCGGGGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACTTT CCTCTACGCG 
GCCTCTGGAA ATGCCGTCAG AATGTGGGAC CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACCGGACACC TGGGTCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA TGGACAGGAC 
CTCATCATCA CTGGCTCAAA AGACCACTAC ATCAAAATGT TCGATGTGAC TGAAGGGGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTC GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGCGATAA CCTATTCAGT GGGTCCAGAG ATAATGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGTCT TCTTCAGCAA GTCCCAAATG CACACAAAGA CTGGGTGTGT 
GCCCTGGGCC TGGTGCCAGG CCATCCGGTT TTGCTGAGTG GCTGCAGAGG CGGCATTCTG 
AAACTCTGGA ATGTGGACAC TTTTGTGCCC GTTGGAGAGA TGAGAGGTCA TGACAGTCCC 
ATCAATGCCA TTTGTGTTAA CTCCACCCAT GTCTTTACTG CTGCTGATGA TCGAACCGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAACTCT CTGACACCGG CGATCTGGGG 
GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171953.1
CDS	9004..13824
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316464.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316464.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG CAGATCCACC AGAAGAGGCA TAATCAACCC ATTTCCTGCC 
TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG TGCGTTCACA TAGCTGAAGG GCACACCAAG 
GCTGTGCTCT GTGTGGATTC TACGGATGAT CTTCTCTTCA CCGGATCAAA AGATCGCACT 
TGTAAAGTAT GGAATCTCGT GACTGGACAG GAAATTATGT CCCTGGGGGT TCACCCTAAC 
AACGTGGTGT CTGTAAAATA CTGTAATTAT ACCAGTCTGG TCTTCACCGT GTCGACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCAAAATGCA TTCGAACATT AACATCTTCA 
GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT GCCAGCACCA GCCGGACAGT AGCTATTCCT 
TCCGGGGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACTTT CCTCTACGCG 
GCCTCTGGAA ATGCCGTCAG AATGTGGGAC CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACCGGACACC TGGGTCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA TGGACAGGAC 
CTCATCATCA CTGGCTCAAA AGACCACTAC ATCAAAATGT TCGATGTGAC TGAAGGGGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTC GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGCGATAA CCTATTCAGT GGGTCCAGAG ATAATGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGTCT TCTTCAGCAA GTCCCAAATG CACACAAAGA CTGGGTGTGT 
GCCCTGGGCC TGGTGCCAGG CCATCCGGTT TTGCTGAGTG GCTGCAGAGG CGGCATTCTG 
AAACTCTGGA ATGTGGACAC TTTTGTGCCC GTTGGAGAGA TGAGAGGTCA TGACAGTCCC 
ATCAATGCCA TTTGTGTTAA CTCCACCCAT GTCTTTACTG CTGCTGATGA TCGAACCGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAACTCT CTGACACCGG CGATCTGGGG 
GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316465.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171954.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.

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CloneID	OMu79031
Clone ID Related Accession (Same CDS sequence)	XM_017316465.1 , XM_017316465.2
Accession Version	XM_017316465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171954.1
CDS	9004..13821
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316465.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316465.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316466.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171955.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.

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CloneID	OMu79032
Clone ID Related Accession (Same CDS sequence)	XM_017316466.1 , XM_017316466.2
Accession Version	XM_017316466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171955.1
CDS	9004..13818
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316466.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316466.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316468.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171957.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OMu79034
Clone ID Related Accession (Same CDS sequence)	XM_017316468.1 , XM_017316468.2
Accession Version	XM_017316468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171957.1
CDS	9004..13809
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316468.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316468.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316471.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171960.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu79036
Clone ID Related Accession (Same CDS sequence)	XM_017316471.1 , XM_017316471.2
Accession Version	XM_017316471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT GTCTGATGAA AGTGACTCCT CTTTGTCCGA GGTGCACAGA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171960.1
CDS	9004..13788
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316471.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316471.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT GTCTGATGAA AGTGACTCCT CTTTGTCCGA GGTGCACAGA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316474.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171963.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.

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CloneID	OMu79039
Clone ID Related Accession (Same CDS sequence)	XM_017316474.2 , XM_017316474.1
Accession Version	XM_017316474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171963.1
CDS	9004..13770
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316474.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316474.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316475.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171964.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.

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CloneID	OMu79040
Clone ID Related Accession (Same CDS sequence)	XM_017316475.2 , XM_017316475.1
Accession Version	XM_017316475.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171964.1
CDS	9004..13770
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316475.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316475.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316476.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171965.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.

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CloneID	OMu79041
Clone ID Related Accession (Same CDS sequence)	XM_017316476.1 , XM_017316476.2
Accession Version	XM_017316476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171965.1
CDS	9004..13749
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316476.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316476.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316477.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171966.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OMu79042
Clone ID Related Accession (Same CDS sequence)	XM_017316477.2 , XM_017316477.1
Accession Version	XM_017316477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171966.1
CDS	9004..13749
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316477.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316477.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316478.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171967.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu79043
Clone ID Related Accession (Same CDS sequence)	XM_017316478.1 , XM_017316478.2
Accession Version	XM_017316478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACCG GATTTGACGT GAACATCATG GAGGAAGAGC AGGGCATCAT CTCTCGTGCT 
GTTAGACACC TGTTCAAGAG TATTGATGAG AAAAAGACCT CAGCGATTAA AAACGGGCTG 
CCCCCTCCTG AATTCAAAGT GAATGCCCAG TTCCTAGAGC TCTATAATGA AGAGGTCCTT 
GACTTGTTTG ATACCACTCG GGATATTGAT GCAAAAAATA AAAAATCAAA TATAAGAATT 
CATGAAGATT CAACTGGAGG AATTTATACT GTGGGCGTCA CAACACGCAC TGTGAATACA 
GAACCGGAGA TGATGCAGTG TCTGAAGCTG GGCGCTCTCT CGCGCACCAC GGCCAGCACC 
CAGATGAACG TACAGAGCTC TCGTTCACAC GCCATCTTTA CCATTCACGT GTGTCAAACC 
AGAGTGTGTC CCCAAACAGA TGCTGAGAAC GCAACTGATA ATAAGCTGAT CTCCGAATCG 
TCGCCAATGA ATGAGTTTGA GACGCTGACG GCGAAGTTTC ACTTTGTTGA TCTGGCGGGA 
TCGGAAAGAC TGAAGAGAAC CGGAGCTACA GGCGAGAGAG CAAAAGAGGG CATTTCCATC 
AACTGCGGGC TTTTGGCTCT TGGGAATGTA ATCAGCGCCT TGGGGGACAA GAGCAAGAGA 
GCCACCCATG TCCCTTACAG AGACTCTAAG CTGACAAGAC TCTTGCAGGA TTCCCTTGGG 
GGTAACAGCC AAACCATCAT GATAGCATGC GTCAGCCCTT CAGACAGGGA TTTCATGGAG 
ACGTTAAACA CTCTGAAGTA CGCCAATCGA GCGAGAAATA TCAAGAACAA GGTGATGGTC 
AATCAAGACA GAGCCAGTCA GCAAATCAAC GCGCTGCGGA GCGAGATCAC GCGCCTTCAG 
ATGGAGCTCA TGGAATACAA AACCGGTAAA AGAATAATTG ACGAGGAAGG CGTGGAAAGC 
ATCAATGACA TGTTTCATGA GAATGCTATG CTGCAGACGG AAAATAATAA TCTGCGTGTA 
AGAATAAAAG CCATGCAGGA GACCGTTGAT GCACTGAGGG CCAGAATCAC GCAGCTTGTC 
AGTGAGCAGG CCAACCAAGT TCTTGCCCGA GCAGGTGAAG GGAACGAAGA GATCAGTAAT 
ATGATTCATA GTTATATCAA AGAAATTGAA GACCTCAGGG CAAAATTATT AGAAAGTGAA 
GCAGTGAACG AGAACCTTCG GAAGAACTTG ACCAGAGCCA CGGCGAGATC TCCTTACTTC 
AGTGCCTCCT CAGCTTTCTC GCCTACTATA CTGTCTTCAG ACAAGGAGAC TATCGAAATT 
ATAGATCTAG CAAAGAAAGA CTTGGAGAAG CTAAAACGGA AAGAGAAGAA GAAGAAGAAA 
AGGCTACAGA AACTTGAGGA AAGCGGTCGA GAAGAAAGAA GTGTTGCCGG GAAAGACGAT 
AATGCAGACA CTGACCAGGA GAAGAAAGAA GAAAAGGGTG TTTCAGAGAA AGAAAACAAT 
GAGCTAGACG TGGAAGAGAA TCAAGAAGTG AGTGACCACG AGGATGAGGA AGAGGAGGAA 
GAGGACGAGG AGGAAGAGGA TGACATTGAA GGAGAAGAAA GCTCTGATGA ATCAGATTCC 
GAATCTGATG AAAAAGCTAA CTATCAAGCC GACTTAGCAA ATATCACCTG CGAGATTGCG 
ATTAAGCAGA AGCTGATCGA CGAACTGGAG AACAGCCAGA AACGGCTGCA GACCCTGAAA 
AAGCAGTACG AGGAGAAGCT GATGATGCTC CAACATAAGA TCCGGGACAC GCAGCTGGAG 
AGGGACCAGG TGCTCCAGAA CCTAGGCTCG GTGGAGTCAT ACTCGGAAGA AAAGGCCAAG 
AAAGTGAAGT GCGAATATGA GAAGAAGCTC CACGCCATGA ACAAAGAGCT GCAGCGACTG 
CAGACGGCCC AAAAGGAGCA CGCCAGGCTC CTCAAAAACC AGTCTCAGTA TGAAAAGCAG 
CTCAAGAAAC TGCAGCAAGA CGTCATGGAA ATGAAGAAAA CCAAGGTTCG TCTAATGAAG 
CAGATGAAAG AAGAGCAGGA GAAAGCCCGG CTGACAGAAT CTCGAAGGAA CCGGGAAATC 
GCTCAGCTGA AGAAGGATCA GCGCAAGCGA GATCATCAAC TTAGACTTCT AGAGGCCCAG 
AAAAGAAATC AAGAAGTAGT TCTGCGACGC AAAACGGAAG AGGTTACAGC TCTCCGCAGG 
CAAGTGAGGC CCATGTCTGA TAAAGTAGCG GGAAAAGTTA CTCGGAAGCT GAGCTCATCC 
GAAAGCCCCG CTCCGGACAC AGGTTCCAGT GCGGCTTCCG GGGAAGCAGA CACATCACGG 
CCAGGCACCC AGCAGAAAAT GAGGATCCCC GTGGCAAGAG TCCAGGCATT ACCAACACCT 
ACAACAAATG GCACCAGGAA AAAATATCAG AGGAAAGGAT TCACTGGCCG GGTGTTCACT 
TCCAAGACAG CCCGCATGAA GTGGCAGCTA CTGGAGCGCC GGGTGACCGA CATCATCATG 
CAGAAAATGA CCATCTCCAA CATGGAGGCG GACATGAACA GACTCCTCAG GCAACGGGAA 
GAACTCACAA AAAGGCGAGA GAAACTTTCT AAAAGGAGAG AGAAGATAGT CAAGGAGAGC 
GGAGAGGGAG ATAAAAGTGT GGCTAACATC ATCGAGGAGA TGGAGTCCCT GACAGCCAAC 
ATAGATTACA TCAATGATAG CATTGCCGAC TGTCAAGCCA ACATCATGCA GATGGAGGAG 
GCAAAGGAAG AAGGGGAGAC ATTGGATGTC ACCGCTGTCA TTAATGCCTG TACACTGACA 
GAAGCTCGGT ACCTGCTAGA TCACTTCCTG TCAATGGGCA TCAATAAGGG TCTGCAGGCT 
GCCCAGAAAG AGGCTCAAAT TAAAGTCCTC GAGGGTCGAC TCAAACAGAC CGAAATCACC 
AGTGCAACCC AGAACCAACT CTTATTCCAT ATGCTGAAGG AGAAGGCAGA GCTAAACCCA 
GAGCTGGATG CCTTGCTAGG CCACGCACTG CAAGATCTAG ATGGCGCCCC ACCAGAAAAC 
GAGGAGGACA GCAGCGAGGA AGATGGCCCT TTACACAGCC CGGGGTCGGA GGGAAGCACG 
TTATCTTCAG ACCTTATGAA GCTTTGTGGT GAAGTGAAAC CAAAGAACAA GGCTCGAAGG 
AGAACCACCA CTCAGATGGA GTTGCTGTAT GCAGATAGCA GTGAAGTAGC CTCAGACACT 
AGTGCAGGAG ATGCCTCCTT GTCTGGCCCT CTGGCACCTG TTGCAGAAGG GCAGGAGATT 
GGAATGAACA CAGAGACAAG TGGTACTTCT GCTAGGGACA AAGAGCTTCT TGCCCCATCT 
GGCTTACCTT CTAAGATAGG CAGCATTTCC AGACAGTCAT CTCTATCAGA AAAAAAAGTG 
CCAGAGCCTT CCCCTGTAAC AAGGAGAAAG GCATATGAGA AGGCAGATAA ACCAAAGGCG 
AAGGAACATA AACACTCAGA TTCGGGCGCT TCCGAGACTA GTCTCTCACC TCCTTCCTCC 
CCACCAAGCC GGCCCCGTAA CGAACTGAAT GTGTTTAATC GCCTTACTGT GCCTCAGGGA 
ACCCCGTCAG TTCAGCAGGA TAAGTCTGAT GAAAGTGACT CCTCTTTGTC CGAGGTGCAC 
AGCAGATCCA CCAGAAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171967.1
CDS	554..5296
Translation	MGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316478.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316478.2

ORF Insert Sequence:

ATGGGAACCG GATTTGACGT GAACATCATG GAGGAAGAGC AGGGCATCAT CTCTCGTGCT 
GTTAGACACC TGTTCAAGAG TATTGATGAG AAAAAGACCT CAGCGATTAA AAACGGGCTG 
CCCCCTCCTG AATTCAAAGT GAATGCCCAG TTCCTAGAGC TCTATAATGA AGAGGTCCTT 
GACTTGTTTG ATACCACTCG GGATATTGAT GCAAAAAATA AAAAATCAAA TATAAGAATT 
CATGAAGATT CAACTGGAGG AATTTATACT GTGGGCGTCA CAACACGCAC TGTGAATACA 
GAACCGGAGA TGATGCAGTG TCTGAAGCTG GGCGCTCTCT CGCGCACCAC GGCCAGCACC 
CAGATGAACG TACAGAGCTC TCGTTCACAC GCCATCTTTA CCATTCACGT GTGTCAAACC 
AGAGTGTGTC CCCAAACAGA TGCTGAGAAC GCAACTGATA ATAAGCTGAT CTCCGAATCG 
TCGCCAATGA ATGAGTTTGA GACGCTGACG GCGAAGTTTC ACTTTGTTGA TCTGGCGGGA 
TCGGAAAGAC TGAAGAGAAC CGGAGCTACA GGCGAGAGAG CAAAAGAGGG CATTTCCATC 
AACTGCGGGC TTTTGGCTCT TGGGAATGTA ATCAGCGCCT TGGGGGACAA GAGCAAGAGA 
GCCACCCATG TCCCTTACAG AGACTCTAAG CTGACAAGAC TCTTGCAGGA TTCCCTTGGG 
GGTAACAGCC AAACCATCAT GATAGCATGC GTCAGCCCTT CAGACAGGGA TTTCATGGAG 
ACGTTAAACA CTCTGAAGTA CGCCAATCGA GCGAGAAATA TCAAGAACAA GGTGATGGTC 
AATCAAGACA GAGCCAGTCA GCAAATCAAC GCGCTGCGGA GCGAGATCAC GCGCCTTCAG 
ATGGAGCTCA TGGAATACAA AACCGGTAAA AGAATAATTG ACGAGGAAGG CGTGGAAAGC 
ATCAATGACA TGTTTCATGA GAATGCTATG CTGCAGACGG AAAATAATAA TCTGCGTGTA 
AGAATAAAAG CCATGCAGGA GACCGTTGAT GCACTGAGGG CCAGAATCAC GCAGCTTGTC 
AGTGAGCAGG CCAACCAAGT TCTTGCCCGA GCAGGTGAAG GGAACGAAGA GATCAGTAAT 
ATGATTCATA GTTATATCAA AGAAATTGAA GACCTCAGGG CAAAATTATT AGAAAGTGAA 
GCAGTGAACG AGAACCTTCG GAAGAACTTG ACCAGAGCCA CGGCGAGATC TCCTTACTTC 
AGTGCCTCCT CAGCTTTCTC GCCTACTATA CTGTCTTCAG ACAAGGAGAC TATCGAAATT 
ATAGATCTAG CAAAGAAAGA CTTGGAGAAG CTAAAACGGA AAGAGAAGAA GAAGAAGAAA 
AGGCTACAGA AACTTGAGGA AAGCGGTCGA GAAGAAAGAA GTGTTGCCGG GAAAGACGAT 
AATGCAGACA CTGACCAGGA GAAGAAAGAA GAAAAGGGTG TTTCAGAGAA AGAAAACAAT 
GAGCTAGACG TGGAAGAGAA TCAAGAAGTG AGTGACCACG AGGATGAGGA AGAGGAGGAA 
GAGGACGAGG AGGAAGAGGA TGACATTGAA GGAGAAGAAA GCTCTGATGA ATCAGATTCC 
GAATCTGATG AAAAAGCTAA CTATCAAGCC GACTTAGCAA ATATCACCTG CGAGATTGCG 
ATTAAGCAGA AGCTGATCGA CGAACTGGAG AACAGCCAGA AACGGCTGCA GACCCTGAAA 
AAGCAGTACG AGGAGAAGCT GATGATGCTC CAACATAAGA TCCGGGACAC GCAGCTGGAG 
AGGGACCAGG TGCTCCAGAA CCTAGGCTCG GTGGAGTCAT ACTCGGAAGA AAAGGCCAAG 
AAAGTGAAGT GCGAATATGA GAAGAAGCTC CACGCCATGA ACAAAGAGCT GCAGCGACTG 
CAGACGGCCC AAAAGGAGCA CGCCAGGCTC CTCAAAAACC AGTCTCAGTA TGAAAAGCAG 
CTCAAGAAAC TGCAGCAAGA CGTCATGGAA ATGAAGAAAA CCAAGGTTCG TCTAATGAAG 
CAGATGAAAG AAGAGCAGGA GAAAGCCCGG CTGACAGAAT CTCGAAGGAA CCGGGAAATC 
GCTCAGCTGA AGAAGGATCA GCGCAAGCGA GATCATCAAC TTAGACTTCT AGAGGCCCAG 
AAAAGAAATC AAGAAGTAGT TCTGCGACGC AAAACGGAAG AGGTTACAGC TCTCCGCAGG 
CAAGTGAGGC CCATGTCTGA TAAAGTAGCG GGAAAAGTTA CTCGGAAGCT GAGCTCATCC 
GAAAGCCCCG CTCCGGACAC AGGTTCCAGT GCGGCTTCCG GGGAAGCAGA CACATCACGG 
CCAGGCACCC AGCAGAAAAT GAGGATCCCC GTGGCAAGAG TCCAGGCATT ACCAACACCT 
ACAACAAATG GCACCAGGAA AAAATATCAG AGGAAAGGAT TCACTGGCCG GGTGTTCACT 
TCCAAGACAG CCCGCATGAA GTGGCAGCTA CTGGAGCGCC GGGTGACCGA CATCATCATG 
CAGAAAATGA CCATCTCCAA CATGGAGGCG GACATGAACA GACTCCTCAG GCAACGGGAA 
GAACTCACAA AAAGGCGAGA GAAACTTTCT AAAAGGAGAG AGAAGATAGT CAAGGAGAGC 
GGAGAGGGAG ATAAAAGTGT GGCTAACATC ATCGAGGAGA TGGAGTCCCT GACAGCCAAC 
ATAGATTACA TCAATGATAG CATTGCCGAC TGTCAAGCCA ACATCATGCA GATGGAGGAG 
GCAAAGGAAG AAGGGGAGAC ATTGGATGTC ACCGCTGTCA TTAATGCCTG TACACTGACA 
GAAGCTCGGT ACCTGCTAGA TCACTTCCTG TCAATGGGCA TCAATAAGGG TCTGCAGGCT 
GCCCAGAAAG AGGCTCAAAT TAAAGTCCTC GAGGGTCGAC TCAAACAGAC CGAAATCACC 
AGTGCAACCC AGAACCAACT CTTATTCCAT ATGCTGAAGG AGAAGGCAGA GCTAAACCCA 
GAGCTGGATG CCTTGCTAGG CCACGCACTG CAAGATCTAG ATGGCGCCCC ACCAGAAAAC 
GAGGAGGACA GCAGCGAGGA AGATGGCCCT TTACACAGCC CGGGGTCGGA GGGAAGCACG 
TTATCTTCAG ACCTTATGAA GCTTTGTGGT GAAGTGAAAC CAAAGAACAA GGCTCGAAGG 
AGAACCACCA CTCAGATGGA GTTGCTGTAT GCAGATAGCA GTGAAGTAGC CTCAGACACT 
AGTGCAGGAG ATGCCTCCTT GTCTGGCCCT CTGGCACCTG TTGCAGAAGG GCAGGAGATT 
GGAATGAACA CAGAGACAAG TGGTACTTCT GCTAGGGACA AAGAGCTTCT TGCCCCATCT 
GGCTTACCTT CTAAGATAGG CAGCATTTCC AGACAGTCAT CTCTATCAGA AAAAAAAGTG 
CCAGAGCCTT CCCCTGTAAC AAGGAGAAAG GCATATGAGA AGGCAGATAA ACCAAAGGCG 
AAGGAACATA AACACTCAGA TTCGGGCGCT TCCGAGACTA GTCTCTCACC TCCTTCCTCC 
CCACCAAGCC GGCCCCGTAA CGAACTGAAT GTGTTTAATC GCCTTACTGT GCCTCAGGGA 
ACCCCGTCAG TTCAGCAGGA TAAGTCTGAT GAAAGTGACT CCTCTTTGTC CGAGGTGCAC 
AGCAGATCCA CCAGAAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316479.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171968.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.

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CloneID	OMu79044
Clone ID Related Accession (Same CDS sequence)	XM_017316479.1 , XM_017316479.2
Accession Version	XM_017316479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATAGGCATAA TCAACCCATT TCCTGCCTGC AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC 
GTTCACATAG CTGAAGGGCA CACCAAGGCT GTGCTCTGTG TGGATTCTAC GGATGATCTT 
CTCTTCACCG GATCAAAAGA TCGCACTTGT AAAGTATGGA ATCTCGTGAC TGGACAGGAA 
ATTATGTCCC TGGGGGTTCA CCCTAACAAC GTGGTGTCTG TAAAATACTG TAATTATACC 
AGTCTGGTCT TCACCGTGTC GACATCTTAT ATTAAGGTGT GGGATATCAG AGAGTCAGCA 
AAATGCATTC GAACATTAAC ATCTTCAGGT CAAGTTACCC TTGGAGAAGC GTGTTCTGCC 
AGCACCAGCC GGACAGTAGC TATTCCTTCC GGGGAGAGCC AGATCAATCA AATTGCACTA 
AACCCAACTG GCACTTTCCT CTACGCGGCC TCTGGAAATG CCGTCAGAAT GTGGGACCTT 
AAAAGGTTTC AGTCTACAGG AAAGCTAACC GGACACCTGG GTCCTGTTAT GTGCCTTACG 
GTAGACCAGA TCTCCAATGG ACAGGACCTC ATCATCACTG GCTCAAAAGA CCACTACATC 
AAAATGTTCG ATGTGACTGA AGGGGCTCTT GGAACTGTAA GTCCCACCCA CAACTTCGAG 
CCTCCTCATT ATGATGGGAT AGAAGCACTG GCCATTCAAG GCGATAACCT ATTCAGTGGG 
TCCAGAGATA ATGGAATCAA GAAATGGGAC TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC 
CCAAATGCAC ACAAAGACTG GGTGTGTGCC CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG 
CTGAGTGGCT GCAGAGGCGG CATTCTGAAA CTCTGGAATG TGGACACTTT TGTGCCCGTT 
GGAGAGATGA GAGGTCATGA CAGTCCCATC AATGCCATTT GTGTTAACTC CACCCATGTC 
TTTACTGCTG CTGATGATCG AACCGTGAGA ATCTGGAAGG CCCACAACTT GCAAGATGGT 
CAACTCTCTG ACACCGGCGA TCTGGGGGAG GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171968.1
CDS	9004..13731
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316479.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316479.2

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATAGGCATAA TCAACCCATT TCCTGCCTGC AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC 
GTTCACATAG CTGAAGGGCA CACCAAGGCT GTGCTCTGTG TGGATTCTAC GGATGATCTT 
CTCTTCACCG GATCAAAAGA TCGCACTTGT AAAGTATGGA ATCTCGTGAC TGGACAGGAA 
ATTATGTCCC TGGGGGTTCA CCCTAACAAC GTGGTGTCTG TAAAATACTG TAATTATACC 
AGTCTGGTCT TCACCGTGTC GACATCTTAT ATTAAGGTGT GGGATATCAG AGAGTCAGCA 
AAATGCATTC GAACATTAAC ATCTTCAGGT CAAGTTACCC TTGGAGAAGC GTGTTCTGCC 
AGCACCAGCC GGACAGTAGC TATTCCTTCC GGGGAGAGCC AGATCAATCA AATTGCACTA 
AACCCAACTG GCACTTTCCT CTACGCGGCC TCTGGAAATG CCGTCAGAAT GTGGGACCTT 
AAAAGGTTTC AGTCTACAGG AAAGCTAACC GGACACCTGG GTCCTGTTAT GTGCCTTACG 
GTAGACCAGA TCTCCAATGG ACAGGACCTC ATCATCACTG GCTCAAAAGA CCACTACATC 
AAAATGTTCG ATGTGACTGA AGGGGCTCTT GGAACTGTAA GTCCCACCCA CAACTTCGAG 
CCTCCTCATT ATGATGGGAT AGAAGCACTG GCCATTCAAG GCGATAACCT ATTCAGTGGG 
TCCAGAGATA ATGGAATCAA GAAATGGGAC TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC 
CCAAATGCAC ACAAAGACTG GGTGTGTGCC CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG 
CTGAGTGGCT GCAGAGGCGG CATTCTGAAA CTCTGGAATG TGGACACTTT TGTGCCCGTT 
GGAGAGATGA GAGGTCATGA CAGTCCCATC AATGCCATTT GTGTTAACTC CACCCATGTC 
TTTACTGCTG CTGATGATCG AACCGTGAGA ATCTGGAAGG CCCACAACTT GCAAGATGGT 
CAACTCTCTG ACACCGGCGA TCTGGGGGAG GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_030248345.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104205.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.

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CloneID	OMu86647
Clone ID Related Accession (Same CDS sequence)	XM_030248345.1
Accession Version	XM_030248345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104205.1
CDS	9004..13857
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_030248345.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248345.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( NM_001109041.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001102511.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant 2, mRNA.

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CloneID	OMu04339
Clone ID Related Accession (Same CDS sequence)	NM_001109041.2
Accession Version	NM_001109041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK019525.1, BY714299.1, BC060698.1, AK147436.1, AK053373.1, AK052821.1 and AC136003.10. On Oct 23, 2013 this sequence version replaced NM_001109041.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147436.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATCT CAGATTCGGG CGCTTCCGAG 
ACTAGTCTCT CACCTCCTTC CTCCCCACCA AGCCGGCCCC GTAACGAACT GAATGTGTTT 
AATCGCCTTA CTGTGCCTCA GGGAACCCCG TCAGTTCAGC AGGATAAGTC TGATGAAAGT 
GACTCCTCTT TGTCCGAGGT GCACAGCAGA TCCACCAGAA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102511.1
CDS	250..5136
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	NM_001109041.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109041.2

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATCT CAGATTCGGG CGCTTCCGAG 
ACTAGTCTCT CACCTCCTTC CTCCCCACCA AGCCGGCCCC GTAACGAACT GAATGTGTTT 
AATCGCCTTA CTGTGCCTCA GGGAACCCCG TCAGTTCAGC AGGATAAGTC TGATGAAAGT 
GACTCCTCTT TGTCCGAGGT GCACAGCAGA TCCACCAGAA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( NM_016705.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057914.2 Mus musculus kinesin family member 21A (Kif21a), transcript variant 3, mRNA.

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CloneID	OMu10411
Clone ID Related Accession (Same CDS sequence)	NM_016705.4
Accession Version	NM_016705.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK019525.1, BY714299.1, BC060698.1, AK147436.1, AK053373.1, AK052821.1 and AC136003.10. On Oct 23, 2013 this sequence version replaced NM_016705.3. Transcript Variant: This variant (3) lacks four alternate exons in the central coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF202892.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATGTC TGATGAAAGT GACTCCTCTT TGTCCGAGGT GCACAGAGGC 
ATAATCAACC CATTTCCTGC CTGCAAAGGA GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC 
ATAGCTGAAG GGCACACCAA GGCTGTGCTC TGTGTGGATT CTACGGATGA TCTTCTCTTC 
ACCGGATCAA AAGATCGCAC TTGTAAAGTA TGGAATCTCG TGACTGGACA GGAAATTATG 
TCCCTGGGGG TTCACCCTAA CAACGTGGTG TCTGTAAAAT ACTGTAATTA TACCAGTCTG 
GTCTTCACCG TGTCGACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCAAAATGC 
ATTCGAACAT TAACATCTTC AGGTCAAGTT ACCCTTGGAG AAGCGTGTTC TGCCAGCACC 
AGCCGGACAG TAGCTATTCC TTCCGGGGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACTT TCCTCTACGC GGCCTCTGGA AATGCCGTCA GAATGTGGGA CCTTAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACCGGACAC CTGGGTCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ATGGACAGGA CCTCATCATC ACTGGCTCAA AAGACCACTA CATCAAAATG 
TTCGATGTGA CTGAAGGGGC TCTTGGAACT GTAAGTCCCA CCCACAACTT CGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGCGATA ACCTATTCAG TGGGTCCAGA 
GATAATGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT 
GCACACAAAG ACTGGGTGTG TGCCCTGGGC CTGGTGCCAG GCCATCCGGT TTTGCTGAGT 
GGCTGCAGAG GCGGCATTCT GAAACTCTGG AATGTGGACA CTTTTGTGCC CGTTGGAGAG 
ATGAGAGGTC ATGACAGTCC CATCAATGCC ATTTGTGTTA ACTCCACCCA TGTCTTTACT 
GCTGCTGATG ATCGAACCGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAACTC 
TCTGACACCG GCGATCTGGG GGAGGATATT GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057914.2
CDS	250..4971
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	NM_016705.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016705.4

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATGTC TGATGAAAGT GACTCCTCTT TGTCCGAGGT GCACAGAGGC 
ATAATCAACC CATTTCCTGC CTGCAAAGGA GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC 
ATAGCTGAAG GGCACACCAA GGCTGTGCTC TGTGTGGATT CTACGGATGA TCTTCTCTTC 
ACCGGATCAA AAGATCGCAC TTGTAAAGTA TGGAATCTCG TGACTGGACA GGAAATTATG 
TCCCTGGGGG TTCACCCTAA CAACGTGGTG TCTGTAAAAT ACTGTAATTA TACCAGTCTG 
GTCTTCACCG TGTCGACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCAAAATGC 
ATTCGAACAT TAACATCTTC AGGTCAAGTT ACCCTTGGAG AAGCGTGTTC TGCCAGCACC 
AGCCGGACAG TAGCTATTCC TTCCGGGGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACTT TCCTCTACGC GGCCTCTGGA AATGCCGTCA GAATGTGGGA CCTTAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACCGGACAC CTGGGTCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ATGGACAGGA CCTCATCATC ACTGGCTCAA AAGACCACTA CATCAAAATG 
TTCGATGTGA CTGAAGGGGC TCTTGGAACT GTAAGTCCCA CCCACAACTT CGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGCGATA ACCTATTCAG TGGGTCCAGA 
GATAATGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT 
GCACACAAAG ACTGGGTGTG TGCCCTGGGC CTGGTGCCAG GCCATCCGGT TTTGCTGAGT 
GGCTGCAGAG GCGGCATTCT GAAACTCTGG AATGTGGACA CTTTTGTGCC CGTTGGAGAG 
ATGAGAGGTC ATGACAGTCC CATCAATGCC ATTTGTGTTA ACTCCACCCA TGTCTTTACT 
GCTGCTGATG ATCGAACCGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAACTC 
TCTGACACCG GCGATCTGGG GGAGGATATT GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( NM_001109040.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001102510.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14016
Clone ID Related Accession (Same CDS sequence)	NM_001109040.2
Accession Version	NM_001109040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK019525.1, BY714299.1, AK163536.1, BC062896.1, AK049303.1, AK147436.1, AK053373.1, AK052821.1 and AC136003.10. On Oct 23, 2013 this sequence version replaced NM_001109040.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGGCTACAG 
AAACTTGAGG AAAGCGGTCG AGAAGAAAGA AGTGTTGCCG GGAAAGACGA TAATGCAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT GTTTCAGAGA AAGAAAACAA TGAGCTAGAC 
GTGGAAGAGA ATCAAGAAGT GAGTGACCAC GAGGATGAGG AAGAGGAGGA AGAGGACGAG 
GAGGAAGAGG ATGACATTGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCTGAT 
GAAAAAGCTA ACTATCAAGC CGACTTAGCA AATATCACCT GCGAGATTGC GATTAAGCAG 
AAGCTGATCG ACGAACTGGA GAACAGCCAG AAACGGCTGC AGACCCTGAA AAAGCAGTAC 
GAGGAGAAGC TGATGATGCT CCAACATAAG ATCCGGGACA CGCAGCTGGA GAGGGACCAG 
GTGCTCCAGA ACCTAGGCTC GGTGGAGTCA TACTCGGAAG AAAAGGCCAA GAAAGTGAAG 
TGCGAATATG AGAAGAAGCT CCACGCCATG AACAAAGAGC TGCAGCGACT GCAGACGGCC 
CAAAAGGAGC ACGCCAGGCT CCTCAAAAAC CAGTCTCAGT ATGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCATGGA AATGAAGAAA ACCAAGGTTC GTCTAATGAA GCAGATGAAA 
GAAGAGCAGG AGAAAGCCCG GCTGACAGAA TCTCGAAGGA ACCGGGAAAT CGCTCAGCTG 
AAGAAGGATC AGCGCAAGCG AGATCATCAA CTTAGACTTC TAGAGGCCCA GAAAAGAAAT 
CAAGAAGTAG TTCTGCGACG CAAAACGGAA GAGGTTACAG CTCTCCGCAG GCAAGTGAGG 
CCCATGTCTG ATAAAGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAAAGCCCC 
GCTCCGGACA CAGGTTCCAG TGCGGCTTCC GGGGAAGCAG ACACATCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATCCC CGTGGCAAGA GTCCAGGCAT TACCAACACC TACAACAAAT 
GGCACCAGGA AAAAATATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACA 
GCCCGCATGA AGTGGCAGCT ACTGGAGCGC CGGGTGACCG ACATCATCAT GCAGAAAATG 
ACCATCTCCA ACATGGAGGC GGACATGAAC AGACTCCTCA GGCAACGGGA AGAACTCACA 
AAAAGGCGAG AGAAACTTTC TAAAAGGAGA GAGAAGATAG TCAAGGAGAG CGGAGAGGGA 
GATAAAAGTG TGGCTAACAT CATCGAGGAG ATGGAGTCCC TGACAGCCAA CATAGATTAC 
ATCAATGATA GCATTGCCGA CTGTCAAGCC AACATCATGC AGATGGAGGA GGCAAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATTAATGCCT GTACACTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTGCAGGC TGCCCAGAAA 
GAGGCTCAAA TTAAAGTCCT CGAGGGTCGA CTCAAACAGA CCGAAATCAC CAGTGCAACC 
CAGAACCAAC TCTTATTCCA TATGCTGAAG GAGAAGGCAG AGCTAAACCC AGAGCTGGAT 
GCCTTGCTAG GCCACGCACT GCAAGATCTA GATGGCGCCC CACCAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATTTC CAGACAGTCA TCTCTATCAG AAAAAAAAGT GCCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAGGCAGATA AACCAAAGGC GAAGGAACAT 
AAACACTCAG ATTCGGGCGC TTCCGAGACT AGTCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACTG TGCCTCAGGG AACCCCGTCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC TCCTCTTTGT CCGAGGTGCA CAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102510.1
CDS	250..5268
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	NM_001109040.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109040.2

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGGCTACAG 
AAACTTGAGG AAAGCGGTCG AGAAGAAAGA AGTGTTGCCG GGAAAGACGA TAATGCAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT GTTTCAGAGA AAGAAAACAA TGAGCTAGAC 
GTGGAAGAGA ATCAAGAAGT GAGTGACCAC GAGGATGAGG AAGAGGAGGA AGAGGACGAG 
GAGGAAGAGG ATGACATTGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCTGAT 
GAAAAAGCTA ACTATCAAGC CGACTTAGCA AATATCACCT GCGAGATTGC GATTAAGCAG 
AAGCTGATCG ACGAACTGGA GAACAGCCAG AAACGGCTGC AGACCCTGAA AAAGCAGTAC 
GAGGAGAAGC TGATGATGCT CCAACATAAG ATCCGGGACA CGCAGCTGGA GAGGGACCAG 
GTGCTCCAGA ACCTAGGCTC GGTGGAGTCA TACTCGGAAG AAAAGGCCAA GAAAGTGAAG 
TGCGAATATG AGAAGAAGCT CCACGCCATG AACAAAGAGC TGCAGCGACT GCAGACGGCC 
CAAAAGGAGC ACGCCAGGCT CCTCAAAAAC CAGTCTCAGT ATGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCATGGA AATGAAGAAA ACCAAGGTTC GTCTAATGAA GCAGATGAAA 
GAAGAGCAGG AGAAAGCCCG GCTGACAGAA TCTCGAAGGA ACCGGGAAAT CGCTCAGCTG 
AAGAAGGATC AGCGCAAGCG AGATCATCAA CTTAGACTTC TAGAGGCCCA GAAAAGAAAT 
CAAGAAGTAG TTCTGCGACG CAAAACGGAA GAGGTTACAG CTCTCCGCAG GCAAGTGAGG 
CCCATGTCTG ATAAAGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAAAGCCCC 
GCTCCGGACA CAGGTTCCAG TGCGGCTTCC GGGGAAGCAG ACACATCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATCCC CGTGGCAAGA GTCCAGGCAT TACCAACACC TACAACAAAT 
GGCACCAGGA AAAAATATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACA 
GCCCGCATGA AGTGGCAGCT ACTGGAGCGC CGGGTGACCG ACATCATCAT GCAGAAAATG 
ACCATCTCCA ACATGGAGGC GGACATGAAC AGACTCCTCA GGCAACGGGA AGAACTCACA 
AAAAGGCGAG AGAAACTTTC TAAAAGGAGA GAGAAGATAG TCAAGGAGAG CGGAGAGGGA 
GATAAAAGTG TGGCTAACAT CATCGAGGAG ATGGAGTCCC TGACAGCCAA CATAGATTAC 
ATCAATGATA GCATTGCCGA CTGTCAAGCC AACATCATGC AGATGGAGGA GGCAAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATTAATGCCT GTACACTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTGCAGGC TGCCCAGAAA 
GAGGCTCAAA TTAAAGTCCT CGAGGGTCGA CTCAAACAGA CCGAAATCAC CAGTGCAACC 
CAGAACCAAC TCTTATTCCA TATGCTGAAG GAGAAGGCAG AGCTAAACCC AGAGCTGGAT 
GCCTTGCTAG GCCACGCACT GCAAGATCTA GATGGCGCCC CACCAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATTTC CAGACAGTCA TCTCTATCAG AAAAAAAAGT GCCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAGGCAGATA AACCAAAGGC GAAGGAACAT 
AAACACTCAG ATTCGGGCGC TTCCGAGACT AGTCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACTG TGCCTCAGGG AACCCCGTCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC TCCTCTTTGT CCGAGGTGCA CAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( NM_001358050.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344979.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant 5, mRNA.

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CloneID	OMu79033
Clone ID Related Accession (Same CDS sequence)	XM_017316467.1 , NM_001358050.1
Accession Version	NM_001358050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113102.8 and AC136003.10. On Nov 16, 2017 this sequence version replaced XM_017316467.1. Transcript Variant: This variant (5) lacks alternate exons in the central coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (5) is shorter, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.83007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACAAGGC ATAATCAACC CATTTCCTGC CTGCAAAGGA 
GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC ATAGCTGAAG GGCACACCAA GGCTGTGCTC 
TGTGTGGATT CTACGGATGA TCTTCTCTTC ACCGGATCAA AAGATCGCAC TTGTAAAGTA 
TGGAATCTCG TGACTGGACA GGAAATTATG TCCCTGGGGG TTCACCCTAA CAACGTGGTG 
TCTGTAAAAT ACTGTAATTA TACCAGTCTG GTCTTCACCG TGTCGACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCAAAATGC ATTCGAACAT TAACATCTTC AGGTCAAGTT 
ACCCTTGGAG AAGCGTGTTC TGCCAGCACC AGCCGGACAG TAGCTATTCC TTCCGGGGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACTT TCCTCTACGC GGCCTCTGGA 
AATGCCGTCA GAATGTGGGA CCTTAAAAGG TTTCAGTCTA CAGGAAAGCT AACCGGACAC 
CTGGGTCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ATGGACAGGA CCTCATCATC 
ACTGGCTCAA AAGACCACTA CATCAAAATG TTCGATGTGA CTGAAGGGGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT CGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGCGATA ACCTATTCAG TGGGTCCAGA GATAATGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT GCACACAAAG ACTGGGTGTG TGCCCTGGGC 
CTGGTGCCAG GCCATCCGGT TTTGCTGAGT GGCTGCAGAG GCGGCATTCT GAAACTCTGG 
AATGTGGACA CTTTTGTGCC CGTTGGAGAG ATGAGAGGTC ATGACAGTCC CATCAATGCC 
ATTTGTGTTA ACTCCACCCA TGTCTTTACT GCTGCTGATG ATCGAACCGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAACTC TCTGACACCG GCGATCTGGG GGAGGATATT 
GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344979.1
CDS	250..5061
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	NM_001358050.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358050.1

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACAAGGC ATAATCAACC CATTTCCTGC CTGCAAAGGA 
GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC ATAGCTGAAG GGCACACCAA GGCTGTGCTC 
TGTGTGGATT CTACGGATGA TCTTCTCTTC ACCGGATCAA AAGATCGCAC TTGTAAAGTA 
TGGAATCTCG TGACTGGACA GGAAATTATG TCCCTGGGGG TTCACCCTAA CAACGTGGTG 
TCTGTAAAAT ACTGTAATTA TACCAGTCTG GTCTTCACCG TGTCGACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCAAAATGC ATTCGAACAT TAACATCTTC AGGTCAAGTT 
ACCCTTGGAG AAGCGTGTTC TGCCAGCACC AGCCGGACAG TAGCTATTCC TTCCGGGGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACTT TCCTCTACGC GGCCTCTGGA 
AATGCCGTCA GAATGTGGGA CCTTAAAAGG TTTCAGTCTA CAGGAAAGCT AACCGGACAC 
CTGGGTCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ATGGACAGGA CCTCATCATC 
ACTGGCTCAA AAGACCACTA CATCAAAATG TTCGATGTGA CTGAAGGGGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT CGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGCGATA ACCTATTCAG TGGGTCCAGA GATAATGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT GCACACAAAG ACTGGGTGTG TGCCCTGGGC 
CTGGTGCCAG GCCATCCGGT TTTGCTGAGT GGCTGCAGAG GCGGCATTCT GAAACTCTGG 
AATGTGGACA CTTTTGTGCC CGTTGGAGAG ATGAGAGGTC ATGACAGTCC CATCAATGCC 
ATTTGTGTTA ACTCCACCCA TGTCTTTACT GCTGCTGATG ATCGAACCGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAACTC TCTGACACCG GCGATCTGGG GGAGGATATT 
GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_006520495.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520558.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.

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CloneID	OMu50979
Clone ID Related Accession (Same CDS sequence)	XM_006520495.3 , XM_006520495.4
Accession Version	XM_006520495.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACACTCA GATTCGGGCG CTTCCGAGAC TAGTCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACT 
GTGCCTCAGG GAACCCCGTC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CTCCTCTTTG 
TCCGAGGTGC ACAGCAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520558.1
CDS	248..5242
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_006520495.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520495.3

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACACTCA GATTCGGGCG CTTCCGAGAC TAGTCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACT 
GTGCCTCAGG GAACCCCGTC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CTCCTCTTTG 
TCCGAGGTGC ACAGCAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316441.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171930.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

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CloneID	OMu79007
Clone ID Related Accession (Same CDS sequence)	XM_017316441.1 , XM_017316441.2
Accession Version	XM_017316441.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGCAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171930.1
CDS	2461..7518
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316441.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316441.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGCAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316442.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171931.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

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CloneID	OMu79008
Clone ID Related Accession (Same CDS sequence)	XM_017316442.1 , XM_017316442.2
Accession Version	XM_017316442.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171931.1
CDS	2461..7515
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316442.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316442.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGATC CACCAGAAGA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316443.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171932.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu79009
Clone ID Related Accession (Same CDS sequence)	XM_017316443.1 , XM_017316443.2
Accession Version	XM_017316443.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171932.1
CDS	2461..7503
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316443.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316443.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAGT CTGATGAAAG TGACTCCTCT 
TTGTCCGAGG TGCACAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171933.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu79010
Clone ID Related Accession (Same CDS sequence)	XM_017316444.1 , XM_017316444.2
Accession Version	XM_017316444.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACACT CAGATTCGGG CGCTTCCGAG ACTAGTCTCT CACCTCCTTC CTCCCCACCA 
AGCCGGCCCC GTAACGAACT GAATGTGTTT AATCGCCTTA CTGTGCCTCA GGGAACCCCG 
TCAGTTCAGC AGGATAAGTC TGATGAAAGT GACTCCTCTT TGTCCGAGGT GCACAGCAGA 
TCCACCAGAA GAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171933.1
CDS	2461..7497
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316444.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACACT CAGATTCGGG CGCTTCCGAG ACTAGTCTCT CACCTCCTTC CTCCCCACCA 
AGCCGGCCCC GTAACGAACT GAATGTGTTT AATCGCCTTA CTGTGCCTCA GGGAACCCCG 
TCAGTTCAGC AGGATAAGTC TGATGAAAGT GACTCCTCTT TGTCCGAGGT GCACAGCAGA 
TCCACCAGAA GAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316445.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171934.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

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CloneID	OMu79011
Clone ID Related Accession (Same CDS sequence)	XM_017316445.1 , XM_017316445.2
Accession Version	XM_017316445.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGGCTACAG 
AAACTTGAGG AAAGCGGTCG AGAAGAAAGA AGTGTTGCCG GGAAAGACGA TAATGCAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT GTTTCAGAGA AAGAAAACAA TGAGCTAGAC 
GTGGAAGAGA ATCAAGAAGT GAGTGACCAC GAGGATGAGG AAGAGGAGGA AGAGGACGAG 
GAGGAAGAGG ATGACATTGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCTGAT 
GAAAAAGCTA ACTATCAAGC CGACTTAGCA AATATCACCT GCGAGATTGC GATTAAGCAG 
AAGCTGATCG ACGAACTGGA GAACAGCCAG AAACGGCTGC AGACCCTGAA AAAGCAGTAC 
GAGGAGAAGC TGATGATGCT CCAACATAAG ATCCGGGACA CGCAGCTGGA GAGGGACCAG 
GTGCTCCAGA ACCTAGGCTC GGTGGAGTCA TACTCGGAAG AAAAGGCCAA GAAAGTGAAG 
TGCGAATATG AGAAGAAGCT CCACGCCATG AACAAAGAGC TGCAGCGACT GCAGACGGCC 
CAAAAGGAGC ACGCCAGGCT CCTCAAAAAC CAGTCTCAGT ATGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCATGGA AATGAAGAAA ACCAAGGTTC GTCTAATGAA GCAGATGAAA 
GAAGAGCAGG AGAAAGCCCG GCTGACAGAA TCTCGAAGGA ACCGGGAAAT CGCTCAGCTG 
AAGAAGGATC AGCGCAAGCG AGATCATCAA CTTAGACTTC TAGAGGCCCA GAAAAGAAAT 
CAAGAAGTAG TTCTGCGACG CAAAACGGAA GAGGTTACAG CTCTCCGCAG GCAAGTGAGG 
CCCATGTCTG ATAAAGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAAAGCCCC 
GCTCCGGACA CAGGTTCCAG TGCGGCTTCC GGGGAAGCAG ACACATCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATCCC CGTGGCAAGA GTCCAGGCAT TACCAACACC TACAACAAAT 
GGCACCAGGA AAAAATATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACA 
GCCCGCATGA AGTGGCAGCT ACTGGAGCGC CGGGTGACCG ACATCATCAT GCAGAAAATG 
ACCATCTCCA ACATGGAGGC GGACATGAAC AGACTCCTCA GGCAACGGGA AGAACTCACA 
AAAAGGCGAG AGAAACTTTC TAAAAGGAGA GAGAAGATAG TCAAGGAGAG CGGAGAGGGA 
GATAAAAGTG TGGCTAACAT CATCGAGGAG ATGGAGTCCC TGACAGCCAA CATAGATTAC 
ATCAATGATA GCATTGCCGA CTGTCAAGCC AACATCATGC AGATGGAGGA GGCAAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATTAATGCCT GTACACTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTGCAGGC TGCCCAGAAA 
GAGGCTCAAA TTAAAGTCCT CGAGGGTCGA CTCAAACAGA CCGAAATCAC CAGTGCAACC 
CAGAACCAAC TCTTATTCCA TATGCTGAAG GAGAAGGCAG AGCTAAACCC AGAGCTGGAT 
GCCTTGCTAG GCCACGCACT GCAAGATCTA GATGGCGCCC CACCAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATTTC CAGACAGTCA TCTCTATCAG AAAAAAAAGT GCCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAGGCAGATA AACCAAAGGC GAAGGAACAT 
AAACACTCAG ATTCGGGCGC TTCCGAGACT AGTCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACTG TGCCTCAGGG AACCCCGTCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC TCCTCTTTGT CCGAGGTGCA CAGCAGATCC 
ACCAGAAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171934.1
CDS	249..5282
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316445.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316445.1

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGGCTACAG 
AAACTTGAGG AAAGCGGTCG AGAAGAAAGA AGTGTTGCCG GGAAAGACGA TAATGCAGAC 
ACTGACCAGG AGAAGAAAGA AGAAAAGGGT GTTTCAGAGA AAGAAAACAA TGAGCTAGAC 
GTGGAAGAGA ATCAAGAAGT GAGTGACCAC GAGGATGAGG AAGAGGAGGA AGAGGACGAG 
GAGGAAGAGG ATGACATTGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCTGAT 
GAAAAAGCTA ACTATCAAGC CGACTTAGCA AATATCACCT GCGAGATTGC GATTAAGCAG 
AAGCTGATCG ACGAACTGGA GAACAGCCAG AAACGGCTGC AGACCCTGAA AAAGCAGTAC 
GAGGAGAAGC TGATGATGCT CCAACATAAG ATCCGGGACA CGCAGCTGGA GAGGGACCAG 
GTGCTCCAGA ACCTAGGCTC GGTGGAGTCA TACTCGGAAG AAAAGGCCAA GAAAGTGAAG 
TGCGAATATG AGAAGAAGCT CCACGCCATG AACAAAGAGC TGCAGCGACT GCAGACGGCC 
CAAAAGGAGC ACGCCAGGCT CCTCAAAAAC CAGTCTCAGT ATGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCATGGA AATGAAGAAA ACCAAGGTTC GTCTAATGAA GCAGATGAAA 
GAAGAGCAGG AGAAAGCCCG GCTGACAGAA TCTCGAAGGA ACCGGGAAAT CGCTCAGCTG 
AAGAAGGATC AGCGCAAGCG AGATCATCAA CTTAGACTTC TAGAGGCCCA GAAAAGAAAT 
CAAGAAGTAG TTCTGCGACG CAAAACGGAA GAGGTTACAG CTCTCCGCAG GCAAGTGAGG 
CCCATGTCTG ATAAAGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAAAGCCCC 
GCTCCGGACA CAGGTTCCAG TGCGGCTTCC GGGGAAGCAG ACACATCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATCCC CGTGGCAAGA GTCCAGGCAT TACCAACACC TACAACAAAT 
GGCACCAGGA AAAAATATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACA 
GCCCGCATGA AGTGGCAGCT ACTGGAGCGC CGGGTGACCG ACATCATCAT GCAGAAAATG 
ACCATCTCCA ACATGGAGGC GGACATGAAC AGACTCCTCA GGCAACGGGA AGAACTCACA 
AAAAGGCGAG AGAAACTTTC TAAAAGGAGA GAGAAGATAG TCAAGGAGAG CGGAGAGGGA 
GATAAAAGTG TGGCTAACAT CATCGAGGAG ATGGAGTCCC TGACAGCCAA CATAGATTAC 
ATCAATGATA GCATTGCCGA CTGTCAAGCC AACATCATGC AGATGGAGGA GGCAAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATTAATGCCT GTACACTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTGCAGGC TGCCCAGAAA 
GAGGCTCAAA TTAAAGTCCT CGAGGGTCGA CTCAAACAGA CCGAAATCAC CAGTGCAACC 
CAGAACCAAC TCTTATTCCA TATGCTGAAG GAGAAGGCAG AGCTAAACCC AGAGCTGGAT 
GCCTTGCTAG GCCACGCACT GCAAGATCTA GATGGCGCCC CACCAGAAAA CGAGGAGGAC 
AGCAGCGAGG AAGATGGCCC TTTACACAGC CCGGGGTCGG AGGGAAGCAC GTTATCTTCA 
GACCTTATGA AGCTTTGTGG TGAAGTGAAA CCAAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAGATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GATGCCTCCT TGTCTGGCCC TCTGGCACCT GTTGCAGAAG GGCAGGAGAT TGGAATGAAC 
ACAGAGACAA GTGGTACTTC TGCTAGGGAC AAAGAGCTTC TTGCCCCATC TGGCTTACCT 
TCTAAGATAG GCAGCATTTC CAGACAGTCA TCTCTATCAG AAAAAAAAGT GCCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAGGCAGATA AACCAAAGGC GAAGGAACAT 
AAACACTCAG ATTCGGGCGC TTCCGAGACT AGTCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACTG TGCCTCAGGG AACCCCGTCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC TCCTCTTTGT CCGAGGTGCA CAGCAGATCC 
ACCAGAAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316446.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171935.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.

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CloneID	OMu79012
Clone ID Related Accession (Same CDS sequence)	XM_017316446.2 , XM_017316446.1
Accession Version	XM_017316446.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGCA GATCCACCAG AAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171935.1
CDS	2461..7479
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316446.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316446.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGCA GATCCACCAG AAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316447.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171936.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.

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CloneID	OMu79013
Clone ID Related Accession (Same CDS sequence)	XM_017316447.2 , XM_017316447.1
Accession Version	XM_017316447.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGAT CCACCAGAAG AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171936.1
CDS	2461..7476
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316447.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316447.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAG 
TCTGATGAAA GTGACTCCTC TTTGTCCGAG GTGCACAGAT CCACCAGAAG AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316448.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171937.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.

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CloneID	OMu79014
Clone ID Related Accession (Same CDS sequence)	XM_017316448.1 , XM_017316448.2
Accession Version	XM_017316448.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171937.1
CDS	2461..7464
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316448.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316448.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316449.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171938.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.

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CloneID	OMu79015
Clone ID Related Accession (Same CDS sequence)	XM_017316449.1 , XM_017316449.2
Accession Version	XM_017316449.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171938.1
CDS	2461..7425
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316449.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316449.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA CTCAGATTCG 
GGCGCTTCCG AGACTAGTCT CTCACCTCCT TCCTCCCCAC CAAGCCGGCC CCGTAACGAA 
CTGAATGTGT TTAATCGCCT TACTGTGCCT CAGGGAACCC CGTCAGTTCA GCAGGATAAA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316450.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171939.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu79016
Clone ID Related Accession (Same CDS sequence)	XM_017316450.2 , XM_017316450.1
Accession Version	XM_017316450.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TCTCAGATTC GGGCGCTTCC 
GAGACTAGTC TCTCACCTCC TTCCTCCCCA CCAAGCCGGC CCCGTAACGA ACTGAATGTG 
TTTAATCGCC TTACTGTGCC TCAGGGAACC CCGTCAGTTC AGCAGGATAA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGC AGATCCACCA GAAGAGGCAT AATCAACCCA 
TTTCCTGCCT GCAAAGGAGT CAGAGCCTCT CCTCTTCAGT GCGTTCACAT AGCTGAAGGG 
CACACCAAGG CTGTGCTCTG TGTGGATTCT ACGGATGATC TTCTCTTCAC CGGATCAAAA 
GATCGCACTT GTAAAGTATG GAATCTCGTG ACTGGACAGG AAATTATGTC CCTGGGGGTT 
CACCCTAACA ACGTGGTGTC TGTAAAATAC TGTAATTATA CCAGTCTGGT CTTCACCGTG 
TCGACATCTT ATATTAAGGT GTGGGATATC AGAGAGTCAG CAAAATGCAT TCGAACATTA 
ACATCTTCAG GTCAAGTTAC CCTTGGAGAA GCGTGTTCTG CCAGCACCAG CCGGACAGTA 
GCTATTCCTT CCGGGGAGAG CCAGATCAAT CAAATTGCAC TAAACCCAAC TGGCACTTTC 
CTCTACGCGG CCTCTGGAAA TGCCGTCAGA ATGTGGGACC TTAAAAGGTT TCAGTCTACA 
GGAAAGCTAA CCGGACACCT GGGTCCTGTT ATGTGCCTTA CGGTAGACCA GATCTCCAAT 
GGACAGGACC TCATCATCAC TGGCTCAAAA GACCACTACA TCAAAATGTT CGATGTGACT 
GAAGGGGCTC TTGGAACTGT AAGTCCCACC CACAACTTCG AGCCTCCTCA TTATGATGGG 
ATAGAAGCAC TGGCCATTCA AGGCGATAAC CTATTCAGTG GGTCCAGAGA TAATGGAATC 
AAGAAATGGG ACTTAGCTCA GAAAGGTCTT CTTCAGCAAG TCCCAAATGC ACACAAAGAC 
TGGGTGTGTG CCCTGGGCCT GGTGCCAGGC CATCCGGTTT TGCTGAGTGG CTGCAGAGGC 
GGCATTCTGA AACTCTGGAA TGTGGACACT TTTGTGCCCG TTGGAGAGAT GAGAGGTCAT 
GACAGTCCCA TCAATGCCAT TTGTGTTAAC TCCACCCATG TCTTTACTGC TGCTGATGAT 
CGAACCGTGA GAATCTGGAA GGCCCACAAC TTGCAAGATG GTCAACTCTC TGACACCGGC 
GATCTGGGGG AGGATATTGC CAGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171939.1
CDS	2461..7410
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316450.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316450.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TCTCAGATTC GGGCGCTTCC 
GAGACTAGTC TCTCACCTCC TTCCTCCCCA CCAAGCCGGC CCCGTAACGA ACTGAATGTG 
TTTAATCGCC TTACTGTGCC TCAGGGAACC CCGTCAGTTC AGCAGGATAA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGC AGATCCACCA GAAGAGGCAT AATCAACCCA 
TTTCCTGCCT GCAAAGGAGT CAGAGCCTCT CCTCTTCAGT GCGTTCACAT AGCTGAAGGG 
CACACCAAGG CTGTGCTCTG TGTGGATTCT ACGGATGATC TTCTCTTCAC CGGATCAAAA 
GATCGCACTT GTAAAGTATG GAATCTCGTG ACTGGACAGG AAATTATGTC CCTGGGGGTT 
CACCCTAACA ACGTGGTGTC TGTAAAATAC TGTAATTATA CCAGTCTGGT CTTCACCGTG 
TCGACATCTT ATATTAAGGT GTGGGATATC AGAGAGTCAG CAAAATGCAT TCGAACATTA 
ACATCTTCAG GTCAAGTTAC CCTTGGAGAA GCGTGTTCTG CCAGCACCAG CCGGACAGTA 
GCTATTCCTT CCGGGGAGAG CCAGATCAAT CAAATTGCAC TAAACCCAAC TGGCACTTTC 
CTCTACGCGG CCTCTGGAAA TGCCGTCAGA ATGTGGGACC TTAAAAGGTT TCAGTCTACA 
GGAAAGCTAA CCGGACACCT GGGTCCTGTT ATGTGCCTTA CGGTAGACCA GATCTCCAAT 
GGACAGGACC TCATCATCAC TGGCTCAAAA GACCACTACA TCAAAATGTT CGATGTGACT 
GAAGGGGCTC TTGGAACTGT AAGTCCCACC CACAACTTCG AGCCTCCTCA TTATGATGGG 
ATAGAAGCAC TGGCCATTCA AGGCGATAAC CTATTCAGTG GGTCCAGAGA TAATGGAATC 
AAGAAATGGG ACTTAGCTCA GAAAGGTCTT CTTCAGCAAG TCCCAAATGC ACACAAAGAC 
TGGGTGTGTG CCCTGGGCCT GGTGCCAGGC CATCCGGTTT TGCTGAGTGG CTGCAGAGGC 
GGCATTCTGA AACTCTGGAA TGTGGACACT TTTGTGCCCG TTGGAGAGAT GAGAGGTCAT 
GACAGTCCCA TCAATGCCAT TTGTGTTAAC TCCACCCATG TCTTTACTGC TGCTGATGAT 
CGAACCGTGA GAATCTGGAA GGCCCACAAC TTGCAAGATG GTCAACTCTC TGACACCGGC 
GATCTGGGGG AGGATATTGC CAGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316451.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171940.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.

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CloneID	OMu79017
Clone ID Related Accession (Same CDS sequence)	XM_017316451.1
Accession Version	XM_017316451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TCTCAGATTC GGGCGCTTCC 
GAGACTAGTC TCTCACCTCC TTCCTCCCCA CCAAGCCGGC CCCGTAACGA ACTGAATGTG 
TTTAATCGCC TTACTGTGCC TCAGGGAACC CCGTCAGTTC AGCAGGATAA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGA TCCACCAGAA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171940.1
CDS	2461..7407
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316451.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316451.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TCTCAGATTC GGGCGCTTCC 
GAGACTAGTC TCTCACCTCC TTCCTCCCCA CCAAGCCGGC CCCGTAACGA ACTGAATGTG 
TTTAATCGCC TTACTGTGCC TCAGGGAACC CCGTCAGTTC AGCAGGATAA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGA TCCACCAGAA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171941.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.

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CloneID	OMu79018
Clone ID Related Accession (Same CDS sequence)	XM_017316452.1 , XM_017316452.2
Accession Version	XM_017316452.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171941.1
CDS	2461..7404
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316452.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAC TCAGATTCGG GCGCTTCCGA GACTAGTCTC 
TCACCTCCTT CCTCCCCACC AAGCCGGCCC CGTAACGAAC TGAATGTGTT TAATCGCCTT 
ACTGTGCCTC AGGGAACCCC GTCAGTTCAG CAGGATAAAG GCATAATCAA CCCATTTCCT 
GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT CAGTGCGTTC ACATAGCTGA AGGGCACACC 
AAGGCTGTGC TCTGTGTGGA TTCTACGGAT GATCTTCTCT TCACCGGATC AAAAGATCGC 
ACTTGTAAAG TATGGAATCT CGTGACTGGA CAGGAAATTA TGTCCCTGGG GGTTCACCCT 
AACAACGTGG TGTCTGTAAA ATACTGTAAT TATACCAGTC TGGTCTTCAC CGTGTCGACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCAAAAT GCATTCGAAC ATTAACATCT 
TCAGGTCAAG TTACCCTTGG AGAAGCGTGT TCTGCCAGCA CCAGCCGGAC AGTAGCTATT 
CCTTCCGGGG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC TTTCCTCTAC 
GCGGCCTCTG GAAATGCCGT CAGAATGTGG GACCTTAAAA GGTTTCAGTC TACAGGAAAG 
CTAACCGGAC ACCTGGGTCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAATGGACAG 
GACCTCATCA TCACTGGCTC AAAAGACCAC TACATCAAAA TGTTCGATGT GACTGAAGGG 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTCGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGCGA TAACCTATTC AGTGGGTCCA GAGATAATGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG TCTTCTTCAG CAAGTCCCAA ATGCACACAA AGACTGGGTG 
TGTGCCCTGG GCCTGGTGCC AGGCCATCCG GTTTTGCTGA GTGGCTGCAG AGGCGGCATT 
CTGAAACTCT GGAATGTGGA CACTTTTGTG CCCGTTGGAG AGATGAGAGG TCATGACAGT 
CCCATCAATG CCATTTGTGT TAACTCCACC CATGTCTTTA CTGCTGCTGA TGATCGAACC 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAC TCTCTGACAC CGGCGATCTG 
GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316453.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171942.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.

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CloneID	OMu79019
Clone ID Related Accession (Same CDS sequence)	XM_017316453.1 , XM_017316453.2
Accession Version	XM_017316453.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGCA GATCCACCAG AAGAGGCATA ATCAACCCAT TTCCTGCCTG CAAAGGAGTC 
AGAGCCTCTC CTCTTCAGTG CGTTCACATA GCTGAAGGGC ACACCAAGGC TGTGCTCTGT 
GTGGATTCTA CGGATGATCT TCTCTTCACC GGATCAAAAG ATCGCACTTG TAAAGTATGG 
AATCTCGTGA CTGGACAGGA AATTATGTCC CTGGGGGTTC ACCCTAACAA CGTGGTGTCT 
GTAAAATACT GTAATTATAC CAGTCTGGTC TTCACCGTGT CGACATCTTA TATTAAGGTG 
TGGGATATCA GAGAGTCAGC AAAATGCATT CGAACATTAA CATCTTCAGG TCAAGTTACC 
CTTGGAGAAG CGTGTTCTGC CAGCACCAGC CGGACAGTAG CTATTCCTTC CGGGGAGAGC 
CAGATCAATC AAATTGCACT AAACCCAACT GGCACTTTCC TCTACGCGGC CTCTGGAAAT 
GCCGTCAGAA TGTGGGACCT TAAAAGGTTT CAGTCTACAG GAAAGCTAAC CGGACACCTG 
GGTCCTGTTA TGTGCCTTAC GGTAGACCAG ATCTCCAATG GACAGGACCT CATCATCACT 
GGCTCAAAAG ACCACTACAT CAAAATGTTC GATGTGACTG AAGGGGCTCT TGGAACTGTA 
AGTCCCACCC ACAACTTCGA GCCTCCTCAT TATGATGGGA TAGAAGCACT GGCCATTCAA 
GGCGATAACC TATTCAGTGG GTCCAGAGAT AATGGAATCA AGAAATGGGA CTTAGCTCAG 
AAAGGTCTTC TTCAGCAAGT CCCAAATGCA CACAAAGACT GGGTGTGTGC CCTGGGCCTG 
GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC TGCAGAGGCG GCATTCTGAA ACTCTGGAAT 
GTGGACACTT TTGTGCCCGT TGGAGAGATG AGAGGTCATG ACAGTCCCAT CAATGCCATT 
TGTGTTAACT CCACCCATGT CTTTACTGCT GCTGATGATC GAACCGTGAG AATCTGGAAG 
GCCCACAACT TGCAAGATGG TCAACTCTCT GACACCGGCG ATCTGGGGGA GGATATTGCC 
AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171942.1
CDS	2461..7389
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316453.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316453.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGCA GATCCACCAG AAGAGGCATA ATCAACCCAT TTCCTGCCTG CAAAGGAGTC 
AGAGCCTCTC CTCTTCAGTG CGTTCACATA GCTGAAGGGC ACACCAAGGC TGTGCTCTGT 
GTGGATTCTA CGGATGATCT TCTCTTCACC GGATCAAAAG ATCGCACTTG TAAAGTATGG 
AATCTCGTGA CTGGACAGGA AATTATGTCC CTGGGGGTTC ACCCTAACAA CGTGGTGTCT 
GTAAAATACT GTAATTATAC CAGTCTGGTC TTCACCGTGT CGACATCTTA TATTAAGGTG 
TGGGATATCA GAGAGTCAGC AAAATGCATT CGAACATTAA CATCTTCAGG TCAAGTTACC 
CTTGGAGAAG CGTGTTCTGC CAGCACCAGC CGGACAGTAG CTATTCCTTC CGGGGAGAGC 
CAGATCAATC AAATTGCACT AAACCCAACT GGCACTTTCC TCTACGCGGC CTCTGGAAAT 
GCCGTCAGAA TGTGGGACCT TAAAAGGTTT CAGTCTACAG GAAAGCTAAC CGGACACCTG 
GGTCCTGTTA TGTGCCTTAC GGTAGACCAG ATCTCCAATG GACAGGACCT CATCATCACT 
GGCTCAAAAG ACCACTACAT CAAAATGTTC GATGTGACTG AAGGGGCTCT TGGAACTGTA 
AGTCCCACCC ACAACTTCGA GCCTCCTCAT TATGATGGGA TAGAAGCACT GGCCATTCAA 
GGCGATAACC TATTCAGTGG GTCCAGAGAT AATGGAATCA AGAAATGGGA CTTAGCTCAG 
AAAGGTCTTC TTCAGCAAGT CCCAAATGCA CACAAAGACT GGGTGTGTGC CCTGGGCCTG 
GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC TGCAGAGGCG GCATTCTGAA ACTCTGGAAT 
GTGGACACTT TTGTGCCCGT TGGAGAGATG AGAGGTCATG ACAGTCCCAT CAATGCCATT 
TGTGTTAACT CCACCCATGT CTTTACTGCT GCTGATGATC GAACCGTGAG AATCTGGAAG 
GCCCACAACT TGCAAGATGG TCAACTCTCT GACACCGGCG ATCTGGGGGA GGATATTGCC 
AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316454.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171943.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.

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CloneID	OMu79020
Clone ID Related Accession (Same CDS sequence)	XM_017316454.1 , XM_017316454.2
Accession Version	XM_017316454.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAT CCACCAGAAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171943.1
CDS	2461..7386
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316454.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316454.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAT CCACCAGAAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316455.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171944.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu79021
Clone ID Related Accession (Same CDS sequence)	XM_017316455.1 , XM_017316455.2
Accession Version	XM_017316455.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171944.1
CDS	2461..7374
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316455.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316455.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316456.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171945.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu79022
Clone ID Related Accession (Same CDS sequence)	XM_017316456.1 , XM_017316456.2
Accession Version	XM_017316456.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACTCC TCTTTGTCCG AGGTGCACAG CAGATCCACC 
AGAAGAGGCA TAATCAACCC ATTTCCTGCC TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG 
TGCGTTCACA TAGCTGAAGG GCACACCAAG GCTGTGCTCT GTGTGGATTC TACGGATGAT 
CTTCTCTTCA CCGGATCAAA AGATCGCACT TGTAAAGTAT GGAATCTCGT GACTGGACAG 
GAAATTATGT CCCTGGGGGT TCACCCTAAC AACGTGGTGT CTGTAAAATA CTGTAATTAT 
ACCAGTCTGG TCTTCACCGT GTCGACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCAAAATGCA TTCGAACATT AACATCTTCA GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT 
GCCAGCACCA GCCGGACAGT AGCTATTCCT TCCGGGGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACTTT CCTCTACGCG GCCTCTGGAA ATGCCGTCAG AATGTGGGAC 
CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA ACCGGACACC TGGGTCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA TGGACAGGAC CTCATCATCA CTGGCTCAAA AGACCACTAC 
ATCAAAATGT TCGATGTGAC TGAAGGGGCT CTTGGAACTG TAAGTCCCAC CCACAACTTC 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGCGATAA CCTATTCAGT 
GGGTCCAGAG ATAATGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGTCT TCTTCAGCAA 
GTCCCAAATG CACACAAAGA CTGGGTGTGT GCCCTGGGCC TGGTGCCAGG CCATCCGGTT 
TTGCTGAGTG GCTGCAGAGG CGGCATTCTG AAACTCTGGA ATGTGGACAC TTTTGTGCCC 
GTTGGAGAGA TGAGAGGTCA TGACAGTCCC ATCAATGCCA TTTGTGTTAA CTCCACCCAT 
GTCTTTACTG CTGCTGATGA TCGAACCGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAACTCT CTGACACCGG CGATCTGGGG GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171945.1
CDS	2461..7371
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316456.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316456.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACTCC TCTTTGTCCG AGGTGCACAG CAGATCCACC 
AGAAGAGGCA TAATCAACCC ATTTCCTGCC TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG 
TGCGTTCACA TAGCTGAAGG GCACACCAAG GCTGTGCTCT GTGTGGATTC TACGGATGAT 
CTTCTCTTCA CCGGATCAAA AGATCGCACT TGTAAAGTAT GGAATCTCGT GACTGGACAG 
GAAATTATGT CCCTGGGGGT TCACCCTAAC AACGTGGTGT CTGTAAAATA CTGTAATTAT 
ACCAGTCTGG TCTTCACCGT GTCGACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCAAAATGCA TTCGAACATT AACATCTTCA GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT 
GCCAGCACCA GCCGGACAGT AGCTATTCCT TCCGGGGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACTTT CCTCTACGCG GCCTCTGGAA ATGCCGTCAG AATGTGGGAC 
CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA ACCGGACACC TGGGTCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA TGGACAGGAC CTCATCATCA CTGGCTCAAA AGACCACTAC 
ATCAAAATGT TCGATGTGAC TGAAGGGGCT CTTGGAACTG TAAGTCCCAC CCACAACTTC 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGCGATAA CCTATTCAGT 
GGGTCCAGAG ATAATGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGTCT TCTTCAGCAA 
GTCCCAAATG CACACAAAGA CTGGGTGTGT GCCCTGGGCC TGGTGCCAGG CCATCCGGTT 
TTGCTGAGTG GCTGCAGAGG CGGCATTCTG AAACTCTGGA ATGTGGACAC TTTTGTGCCC 
GTTGGAGAGA TGAGAGGTCA TGACAGTCCC ATCAATGCCA TTTGTGTTAA CTCCACCCAT 
GTCTTTACTG CTGCTGATGA TCGAACCGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAACTCT CTGACACCGG CGATCTGGGG GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316457.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171946.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.

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CloneID	OMu79023
Clone ID Related Accession (Same CDS sequence)	XM_017316457.1
Accession Version	XM_017316457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAGT CTGATGAAAG TGACTCCTCT TTGTCCGAGG TGCACAGATC CACCAGAAGA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171946.1
CDS	2461..7365
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316457.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316457.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAGT CTGATGAAAG TGACTCCTCT TTGTCCGAGG TGCACAGATC CACCAGAAGA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316458.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171947.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.

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CloneID	OMu79024
Clone ID Related Accession (Same CDS sequence)	XM_017316458.2 , XM_017316458.1
Accession Version	XM_017316458.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAGT CTGATGAAAG TGACTCCTCT TTGTCCGAGG TGCACAGAGG CATAATCAAC 
CCATTTCCTG CCTGCAAAGG AGTCAGAGCC TCTCCTCTTC AGTGCGTTCA CATAGCTGAA 
GGGCACACCA AGGCTGTGCT CTGTGTGGAT TCTACGGATG ATCTTCTCTT CACCGGATCA 
AAAGATCGCA CTTGTAAAGT ATGGAATCTC GTGACTGGAC AGGAAATTAT GTCCCTGGGG 
GTTCACCCTA ACAACGTGGT GTCTGTAAAA TACTGTAATT ATACCAGTCT GGTCTTCACC 
GTGTCGACAT CTTATATTAA GGTGTGGGAT ATCAGAGAGT CAGCAAAATG CATTCGAACA 
TTAACATCTT CAGGTCAAGT TACCCTTGGA GAAGCGTGTT CTGCCAGCAC CAGCCGGACA 
GTAGCTATTC CTTCCGGGGA GAGCCAGATC AATCAAATTG CACTAAACCC AACTGGCACT 
TTCCTCTACG CGGCCTCTGG AAATGCCGTC AGAATGTGGG ACCTTAAAAG GTTTCAGTCT 
ACAGGAAAGC TAACCGGACA CCTGGGTCCT GTTATGTGCC TTACGGTAGA CCAGATCTCC 
AATGGACAGG ACCTCATCAT CACTGGCTCA AAAGACCACT ACATCAAAAT GTTCGATGTG 
ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC ACCCACAACT TCGAGCCTCC TCATTATGAT 
GGGATAGAAG CACTGGCCAT TCAAGGCGAT AACCTATTCA GTGGGTCCAG AGATAATGGA 
ATCAAGAAAT GGGACTTAGC TCAGAAAGGT CTTCTTCAGC AAGTCCCAAA TGCACACAAA 
GACTGGGTGT GTGCCCTGGG CCTGGTGCCA GGCCATCCGG TTTTGCTGAG TGGCTGCAGA 
GGCGGCATTC TGAAACTCTG GAATGTGGAC ACTTTTGTGC CCGTTGGAGA GATGAGAGGT 
CATGACAGTC CCATCAATGC CATTTGTGTT AACTCCACCC ATGTCTTTAC TGCTGCTGAT 
GATCGAACCG TGAGAATCTG GAAGGCCCAC AACTTGCAAG ATGGTCAACT CTCTGACACC 
GGCGATCTGG GGGAGGATAT TGCCAGTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171947.1
CDS	2461..7353
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316458.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316458.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT TTCCAGACAG TCATCTCTAT CAGAAAAAAA AGTGCCAGAG 
CCTTCCCCTG TAACAAGGAG AAAGGCATAT GAGAAGGCAG ATAAACCAAA GGCGAAGGAA 
CATAAACAGT CTGATGAAAG TGACTCCTCT TTGTCCGAGG TGCACAGAGG CATAATCAAC 
CCATTTCCTG CCTGCAAAGG AGTCAGAGCC TCTCCTCTTC AGTGCGTTCA CATAGCTGAA 
GGGCACACCA AGGCTGTGCT CTGTGTGGAT TCTACGGATG ATCTTCTCTT CACCGGATCA 
AAAGATCGCA CTTGTAAAGT ATGGAATCTC GTGACTGGAC AGGAAATTAT GTCCCTGGGG 
GTTCACCCTA ACAACGTGGT GTCTGTAAAA TACTGTAATT ATACCAGTCT GGTCTTCACC 
GTGTCGACAT CTTATATTAA GGTGTGGGAT ATCAGAGAGT CAGCAAAATG CATTCGAACA 
TTAACATCTT CAGGTCAAGT TACCCTTGGA GAAGCGTGTT CTGCCAGCAC CAGCCGGACA 
GTAGCTATTC CTTCCGGGGA GAGCCAGATC AATCAAATTG CACTAAACCC AACTGGCACT 
TTCCTCTACG CGGCCTCTGG AAATGCCGTC AGAATGTGGG ACCTTAAAAG GTTTCAGTCT 
ACAGGAAAGC TAACCGGACA CCTGGGTCCT GTTATGTGCC TTACGGTAGA CCAGATCTCC 
AATGGACAGG ACCTCATCAT CACTGGCTCA AAAGACCACT ACATCAAAAT GTTCGATGTG 
ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC ACCCACAACT TCGAGCCTCC TCATTATGAT 
GGGATAGAAG CACTGGCCAT TCAAGGCGAT AACCTATTCA GTGGGTCCAG AGATAATGGA 
ATCAAGAAAT GGGACTTAGC TCAGAAAGGT CTTCTTCAGC AAGTCCCAAA TGCACACAAA 
GACTGGGTGT GTGCCCTGGG CCTGGTGCCA GGCCATCCGG TTTTGCTGAG TGGCTGCAGA 
GGCGGCATTC TGAAACTCTG GAATGTGGAC ACTTTTGTGC CCGTTGGAGA GATGAGAGGT 
CATGACAGTC CCATCAATGC CATTTGTGTT AACTCCACCC ATGTCTTTAC TGCTGCTGAT 
GATCGAACCG TGAGAATCTG GAAGGCCCAC AACTTGCAAG ATGGTCAACT CTCTGACACC 
GGCGATCTGG GGGAGGATAT TGCCAGTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316459.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171948.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.

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CloneID	OMu79025
Clone ID Related Accession (Same CDS sequence)	XM_017316459.1
Accession Version	XM_017316459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGA TCCACCAGAA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171948.1
CDS	2461..7347
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316459.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316459.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA GTCTGATGAA 
AGTGACTCCT CTTTGTCCGA GGTGCACAGA TCCACCAGAA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316460.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171949.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.

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CloneID	OMu79026
Clone ID Related Accession (Same CDS sequence)	XM_017316460.1 , XM_017316460.2
Accession Version	XM_017316460.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171949.1
CDS	2461..7335
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316460.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316460.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316461.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171950.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu79027
Clone ID Related Accession (Same CDS sequence)	XM_017316461.1 , XM_017316461.2
Accession Version	XM_017316461.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171950.1
CDS	2461..7317
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316461.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316461.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATCTCAGATT CGGGCGCTTC CGAGACTAGT CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACTGTGC CTCAGGGAAC CCCGTCAGTT 
CAGCAGGATA AAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316462.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171951.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OMu79028
Clone ID Related Accession (Same CDS sequence)	XM_017316462.1 , XM_017316462.2
Accession Version	XM_017316462.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171951.1
CDS	2461..7314
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316462.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316462.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAG TCTGATGAAA GTGACTCCTC TTTGTCCGAG 
GTGCACAGAG GCATAATCAA CCCATTTCCT GCCTGCAAAG GAGTCAGAGC CTCTCCTCTT 
CAGTGCGTTC ACATAGCTGA AGGGCACACC AAGGCTGTGC TCTGTGTGGA TTCTACGGAT 
GATCTTCTCT TCACCGGATC AAAAGATCGC ACTTGTAAAG TATGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCCCTGGG GGTTCACCCT AACAACGTGG TGTCTGTAAA ATACTGTAAT 
TATACCAGTC TGGTCTTCAC CGTGTCGACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCAAAAT GCATTCGAAC ATTAACATCT TCAGGTCAAG TTACCCTTGG AGAAGCGTGT 
TCTGCCAGCA CCAGCCGGAC AGTAGCTATT CCTTCCGGGG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC TTTCCTCTAC GCGGCCTCTG GAAATGCCGT CAGAATGTGG 
GACCTTAAAA GGTTTCAGTC TACAGGAAAG CTAACCGGAC ACCTGGGTCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAATGGACAG GACCTCATCA TCACTGGCTC AAAAGACCAC 
TACATCAAAA TGTTCGATGT GACTGAAGGG GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTCGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGCGA TAACCTATTC 
AGTGGGTCCA GAGATAATGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG TCTTCTTCAG 
CAAGTCCCAA ATGCACACAA AGACTGGGTG TGTGCCCTGG GCCTGGTGCC AGGCCATCCG 
GTTTTGCTGA GTGGCTGCAG AGGCGGCATT CTGAAACTCT GGAATGTGGA CACTTTTGTG 
CCCGTTGGAG AGATGAGAGG TCATGACAGT CCCATCAATG CCATTTGTGT TAACTCCACC 
CATGTCTTTA CTGCTGCTGA TGATCGAACC GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAC TCTCTGACAC CGGCGATCTG GGGGAGGATA TTGCCAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316463.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171952.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.

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CloneID	OMu79029
Clone ID Related Accession (Same CDS sequence)	XM_017316463.1 , XM_017316463.2
Accession Version	XM_017316463.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171952.1
CDS	2461..7296
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316463.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316463.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATTTCCAGAC AGTCATCTCT ATCAGAAAAA AAAGTGCCAG AGCCTTCCCC TGTAACAAGG 
AGAAAGGCAT ATGAGAAGGC AGATAAACCA AAGGCGAAGG AACATAAACA AGGCATAATC 
AACCCATTTC CTGCCTGCAA AGGAGTCAGA GCCTCTCCTC TTCAGTGCGT TCACATAGCT 
GAAGGGCACA CCAAGGCTGT GCTCTGTGTG GATTCTACGG ATGATCTTCT CTTCACCGGA 
TCAAAAGATC GCACTTGTAA AGTATGGAAT CTCGTGACTG GACAGGAAAT TATGTCCCTG 
GGGGTTCACC CTAACAACGT GGTGTCTGTA AAATACTGTA ATTATACCAG TCTGGTCTTC 
ACCGTGTCGA CATCTTATAT TAAGGTGTGG GATATCAGAG AGTCAGCAAA ATGCATTCGA 
ACATTAACAT CTTCAGGTCA AGTTACCCTT GGAGAAGCGT GTTCTGCCAG CACCAGCCGG 
ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG ATCAATCAAA TTGCACTAAA CCCAACTGGC 
ACTTTCCTCT ACGCGGCCTC TGGAAATGCC GTCAGAATGT GGGACCTTAA AAGGTTTCAG 
TCTACAGGAA AGCTAACCGG ACACCTGGGT CCTGTTATGT GCCTTACGGT AGACCAGATC 
TCCAATGGAC AGGACCTCAT CATCACTGGC TCAAAAGACC ACTACATCAA AATGTTCGAT 
GTGACTGAAG GGGCTCTTGG AACTGTAAGT CCCACCCACA ACTTCGAGCC TCCTCATTAT 
GATGGGATAG AAGCACTGGC CATTCAAGGC GATAACCTAT TCAGTGGGTC CAGAGATAAT 
GGAATCAAGA AATGGGACTT AGCTCAGAAA GGTCTTCTTC AGCAAGTCCC AAATGCACAC 
AAAGACTGGG TGTGTGCCCT GGGCCTGGTG CCAGGCCATC CGGTTTTGCT GAGTGGCTGC 
AGAGGCGGCA TTCTGAAACT CTGGAATGTG GACACTTTTG TGCCCGTTGG AGAGATGAGA 
GGTCATGACA GTCCCATCAA TGCCATTTGT GTTAACTCCA CCCATGTCTT TACTGCTGCT 
GATGATCGAA CCGTGAGAAT CTGGAAGGCC CACAACTTGC AAGATGGTCA ACTCTCTGAC 
ACCGGCGATC TGGGGGAGGA TATTGCCAGT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316464.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171953.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.

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CloneID	OMu79030
Clone ID Related Accession (Same CDS sequence)	XM_017316464.1 , XM_017316464.2
Accession Version	XM_017316464.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG CAGATCCACC AGAAGAGGCA TAATCAACCC ATTTCCTGCC 
TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG TGCGTTCACA TAGCTGAAGG GCACACCAAG 
GCTGTGCTCT GTGTGGATTC TACGGATGAT CTTCTCTTCA CCGGATCAAA AGATCGCACT 
TGTAAAGTAT GGAATCTCGT GACTGGACAG GAAATTATGT CCCTGGGGGT TCACCCTAAC 
AACGTGGTGT CTGTAAAATA CTGTAATTAT ACCAGTCTGG TCTTCACCGT GTCGACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCAAAATGCA TTCGAACATT AACATCTTCA 
GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT GCCAGCACCA GCCGGACAGT AGCTATTCCT 
TCCGGGGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACTTT CCTCTACGCG 
GCCTCTGGAA ATGCCGTCAG AATGTGGGAC CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACCGGACACC TGGGTCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA TGGACAGGAC 
CTCATCATCA CTGGCTCAAA AGACCACTAC ATCAAAATGT TCGATGTGAC TGAAGGGGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTC GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGCGATAA CCTATTCAGT GGGTCCAGAG ATAATGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGTCT TCTTCAGCAA GTCCCAAATG CACACAAAGA CTGGGTGTGT 
GCCCTGGGCC TGGTGCCAGG CCATCCGGTT TTGCTGAGTG GCTGCAGAGG CGGCATTCTG 
AAACTCTGGA ATGTGGACAC TTTTGTGCCC GTTGGAGAGA TGAGAGGTCA TGACAGTCCC 
ATCAATGCCA TTTGTGTTAA CTCCACCCAT GTCTTTACTG CTGCTGATGA TCGAACCGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAACTCT CTGACACCGG CGATCTGGGG 
GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171953.1
CDS	2461..7281
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316464.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316464.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG CAGATCCACC AGAAGAGGCA TAATCAACCC ATTTCCTGCC 
TGCAAAGGAG TCAGAGCCTC TCCTCTTCAG TGCGTTCACA TAGCTGAAGG GCACACCAAG 
GCTGTGCTCT GTGTGGATTC TACGGATGAT CTTCTCTTCA CCGGATCAAA AGATCGCACT 
TGTAAAGTAT GGAATCTCGT GACTGGACAG GAAATTATGT CCCTGGGGGT TCACCCTAAC 
AACGTGGTGT CTGTAAAATA CTGTAATTAT ACCAGTCTGG TCTTCACCGT GTCGACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCAAAATGCA TTCGAACATT AACATCTTCA 
GGTCAAGTTA CCCTTGGAGA AGCGTGTTCT GCCAGCACCA GCCGGACAGT AGCTATTCCT 
TCCGGGGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACTTT CCTCTACGCG 
GCCTCTGGAA ATGCCGTCAG AATGTGGGAC CTTAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACCGGACACC TGGGTCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA TGGACAGGAC 
CTCATCATCA CTGGCTCAAA AGACCACTAC ATCAAAATGT TCGATGTGAC TGAAGGGGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTC GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGCGATAA CCTATTCAGT GGGTCCAGAG ATAATGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGTCT TCTTCAGCAA GTCCCAAATG CACACAAAGA CTGGGTGTGT 
GCCCTGGGCC TGGTGCCAGG CCATCCGGTT TTGCTGAGTG GCTGCAGAGG CGGCATTCTG 
AAACTCTGGA ATGTGGACAC TTTTGTGCCC GTTGGAGAGA TGAGAGGTCA TGACAGTCCC 
ATCAATGCCA TTTGTGTTAA CTCCACCCAT GTCTTTACTG CTGCTGATGA TCGAACCGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAACTCT CTGACACCGG CGATCTGGGG 
GAGGATATTG CCAGTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316465.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171954.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.

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CloneID	OMu79031
Clone ID Related Accession (Same CDS sequence)	XM_017316465.1 , XM_017316465.2
Accession Version	XM_017316465.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171954.1
CDS	2461..7278
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316465.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316465.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG ATCCACCAGA AGAGGCATAA TCAACCCATT TCCTGCCTGC 
AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC GTTCACATAG CTGAAGGGCA CACCAAGGCT 
GTGCTCTGTG TGGATTCTAC GGATGATCTT CTCTTCACCG GATCAAAAGA TCGCACTTGT 
AAAGTATGGA ATCTCGTGAC TGGACAGGAA ATTATGTCCC TGGGGGTTCA CCCTAACAAC 
GTGGTGTCTG TAAAATACTG TAATTATACC AGTCTGGTCT TCACCGTGTC GACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCA AAATGCATTC GAACATTAAC ATCTTCAGGT 
CAAGTTACCC TTGGAGAAGC GTGTTCTGCC AGCACCAGCC GGACAGTAGC TATTCCTTCC 
GGGGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACTTTCCT CTACGCGGCC 
TCTGGAAATG CCGTCAGAAT GTGGGACCTT AAAAGGTTTC AGTCTACAGG AAAGCTAACC 
GGACACCTGG GTCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAATGG ACAGGACCTC 
ATCATCACTG GCTCAAAAGA CCACTACATC AAAATGTTCG ATGTGACTGA AGGGGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTCGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GCGATAACCT ATTCAGTGGG TCCAGAGATA ATGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC CCAAATGCAC ACAAAGACTG GGTGTGTGCC 
CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG CTGAGTGGCT GCAGAGGCGG CATTCTGAAA 
CTCTGGAATG TGGACACTTT TGTGCCCGTT GGAGAGATGA GAGGTCATGA CAGTCCCATC 
AATGCCATTT GTGTTAACTC CACCCATGTC TTTACTGCTG CTGATGATCG AACCGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAACTCTCTG ACACCGGCGA TCTGGGGGAG 
GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316466.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171955.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu79032
Clone ID Related Accession (Same CDS sequence)	XM_017316466.1 , XM_017316466.2
Accession Version	XM_017316466.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171955.1
CDS	2461..7275
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316466.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316466.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TTTCCAGACA GTCATCTCTA 
TCAGAAAAAA AAGTGCCAGA GCCTTCCCCT GTAACAAGGA GAAAGGCATA TGAGAAGGCA 
GATAAACCAA AGGCGAAGGA ACATAAACAA GGCATAATCA ACCCATTTCC TGCCTGCAAA 
GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT CACATAGCTG AAGGGCACAC CAAGGCTGTG 
CTCTGTGTGG ATTCTACGGA TGATCTTCTC TTCACCGGAT CAAAAGATCG CACTTGTAAA 
GTATGGAATC TCGTGACTGG ACAGGAAATT ATGTCCCTGG GGGTTCACCC TAACAACGTG 
GTGTCTGTAA AATACTGTAA TTATACCAGT CTGGTCTTCA CCGTGTCGAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCAAAA TGCATTCGAA CATTAACATC TTCAGGTCAA 
GTTACCCTTG GAGAAGCGTG TTCTGCCAGC ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CTTTCCTCTA CGCGGCCTCT 
GGAAATGCCG TCAGAATGTG GGACCTTAAA AGGTTTCAGT CTACAGGAAA GCTAACCGGA 
CACCTGGGTC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAATGGACA GGACCTCATC 
ATCACTGGCT CAAAAGACCA CTACATCAAA ATGTTCGATG TGACTGAAGG GGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTCGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGCG ATAACCTATT CAGTGGGTCC AGAGATAATG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA AATGCACACA AAGACTGGGT GTGTGCCCTG 
GGCCTGGTGC CAGGCCATCC GGTTTTGCTG AGTGGCTGCA GAGGCGGCAT TCTGAAACTC 
TGGAATGTGG ACACTTTTGT GCCCGTTGGA GAGATGAGAG GTCATGACAG TCCCATCAAT 
GCCATTTGTG TTAACTCCAC CCATGTCTTT ACTGCTGCTG ATGATCGAAC CGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA CTCTCTGACA CCGGCGATCT GGGGGAGGAT 
ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316467.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171956.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu79033
Clone ID Related Accession (Same CDS sequence)	XM_017316467.1 , NM_001358050.1
Accession Version	XM_017316467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	kinesin-like protein KIF21A isoform X28
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACAAGGC ATAATCAACC CATTTCCTGC CTGCAAAGGA 
GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC ATAGCTGAAG GGCACACCAA GGCTGTGCTC 
TGTGTGGATT CTACGGATGA TCTTCTCTTC ACCGGATCAA AAGATCGCAC TTGTAAAGTA 
TGGAATCTCG TGACTGGACA GGAAATTATG TCCCTGGGGG TTCACCCTAA CAACGTGGTG 
TCTGTAAAAT ACTGTAATTA TACCAGTCTG GTCTTCACCG TGTCGACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCAAAATGC ATTCGAACAT TAACATCTTC AGGTCAAGTT 
ACCCTTGGAG AAGCGTGTTC TGCCAGCACC AGCCGGACAG TAGCTATTCC TTCCGGGGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACTT TCCTCTACGC GGCCTCTGGA 
AATGCCGTCA GAATGTGGGA CCTTAAAAGG TTTCAGTCTA CAGGAAAGCT AACCGGACAC 
CTGGGTCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ATGGACAGGA CCTCATCATC 
ACTGGCTCAA AAGACCACTA CATCAAAATG TTCGATGTGA CTGAAGGGGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT CGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGCGATA ACCTATTCAG TGGGTCCAGA GATAATGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT GCACACAAAG ACTGGGTGTG TGCCCTGGGC 
CTGGTGCCAG GCCATCCGGT TTTGCTGAGT GGCTGCAGAG GCGGCATTCT GAAACTCTGG 
AATGTGGACA CTTTTGTGCC CGTTGGAGAG ATGAGAGGTC ATGACAGTCC CATCAATGCC 
ATTTGTGTTA ACTCCACCCA TGTCTTTACT GCTGCTGATG ATCGAACCGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAACTC TCTGACACCG GCGATCTGGG GGAGGATATT 
GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171956.1
CDS	247..5058
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKQGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316467.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316467.1

ORF Insert Sequence:

ATGTTGGGCG CTGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATAAG ACCACAGCTT 
GCCAAAGAGA AGATCGAAGG TTGCCATATC TGCACGTCAG TCACACCGGG AGAGCCTCAG 
GTCTTCCTCG GGAAGGATAA GGCCTTTACT TTTGATTATG TGTTTGACAT TGACTCCCAG 
CAGGAGCAGA TCTACACCCA GTGCATCGAA AAGCTCATTG AAGGCTGTTT TGAAGGCTAC 
AATGCCACCG TGTTTGCCTA TGGACAAACC GGAGCTGGGA AAACCTACAC GATGGGAACC 
GGATTTGACG TGAACATCAT GGAGGAAGAG CAGGGCATCA TCTCTCGTGC TGTTAGACAC 
CTGTTCAAGA GTATTGATGA GAAAAAGACC TCAGCGATTA AAAACGGGCT GCCCCCTCCT 
GAATTCAAAG TGAATGCCCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GATACCACTC GGGATATTGA TGCAAAAAAT AAAAAATCAA ATATAAGAAT TCATGAAGAT 
TCAACTGGAG GAATTTATAC TGTGGGCGTC ACAACACGCA CTGTGAATAC AGAACCGGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCCAGCAC CCAGATGAAC 
GTACAGAGCT CTCGTTCACA CGCCATCTTT ACCATTCACG TGTGTCAAAC CAGAGTGTGT 
CCCCAAACAG ATGCTGAGAA CGCAACTGAT AATAAGCTGA TCTCCGAATC GTCGCCAATG 
AATGAGTTTG AGACGCTGAC GGCGAAGTTT CACTTTGTTG ATCTGGCGGG ATCGGAAAGA 
CTGAAGAGAA CCGGAGCTAC AGGCGAGAGA GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGGAATGT AATCAGCGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAT 
GTCCCTTACA GAGACTCTAA GCTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGTAACAGC 
CAAACCATCA TGATAGCATG CGTCAGCCCT TCAGACAGGG ATTTCATGGA GACGTTAAAC 
ACTCTGAAGT ACGCCAATCG AGCGAGAAAT ATCAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA CGCGCTGCGG AGCGAGATCA CGCGCCTTCA GATGGAGCTC 
ATGGAATACA AAACCGGTAA AAGAATAATT GACGAGGAAG GCGTGGAAAG CATCAATGAC 
ATGTTTCATG AGAATGCTAT GCTGCAGACG GAAAATAATA ATCTGCGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCACTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAACGAAG AGATCAGTAA TATGATTCAT 
AGTTATATCA AAGAAATTGA AGACCTCAGG GCAAAATTAT TAGAAAGTGA AGCAGTGAAC 
GAGAACCTTC GGAAGAACTT GACCAGAGCC ACGGCGAGAT CTCCTTACTT CAGTGCCTCC 
TCAGCTTTCT CGCCTACTAT ACTGTCTTCA GACAAGGAGA CTATCGAAAT TATAGATCTA 
GCAAAGAAAG ACTTGGAGAA GCTAAAACGG AAAGAGAAGA AGAAGAAGAA AAGTGTTGCC 
GGGAAAGACG ATAATGCAGA CACTGACCAG GAGAAGAAAG AAGAAAAGGG TGTTTCAGAG 
AAAGAAAACA ATGAGCTAGA CGTGGAAGAG AATCAAGAAG TGAGTGACCA CGAGGATGAG 
GAAGAGGAGG AAGAGGACGA GGAGGAAGAG GATGACATTG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCTGA TGAAAAAGCT AACTATCAAG CCGACTTAGC AAATATCACC 
TGCGAGATTG CGATTAAGCA GAAGCTGATC GACGAACTGG AGAACAGCCA GAAACGGCTG 
CAGACCCTGA AAAAGCAGTA CGAGGAGAAG CTGATGATGC TCCAACATAA GATCCGGGAC 
ACGCAGCTGG AGAGGGACCA GGTGCTCCAG AACCTAGGCT CGGTGGAGTC ATACTCGGAA 
GAAAAGGCCA AGAAAGTGAA GTGCGAATAT GAGAAGAAGC TCCACGCCAT GAACAAAGAG 
CTGCAGCGAC TGCAGACGGC CCAAAAGGAG CACGCCAGGC TCCTCAAAAA CCAGTCTCAG 
TATGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCATGG AAATGAAGAA AACCAAGGTT 
CGTCTAATGA AGCAGATGAA AGAAGAGCAG GAGAAAGCCC GGCTGACAGA ATCTCGAAGG 
AACCGGGAAA TCGCTCAGCT GAAGAAGGAT CAGCGCAAGC GAGATCATCA ACTTAGACTT 
CTAGAGGCCC AGAAAAGAAA TCAAGAAGTA GTTCTGCGAC GCAAAACGGA AGAGGTTACA 
GCTCTCCGCA GGCAAGTGAG GCCCATGTCT GATAAAGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAAAGCCC CGCTCCGGAC ACAGGTTCCA GTGCGGCTTC CGGGGAAGCA 
GACACATCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATCC CCGTGGCAAG AGTCCAGGCA 
TTACCAACAC CTACAACAAA TGGCACCAGG AAAAAATATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC AGCCCGCATG AAGTGGCAGC TACTGGAGCG CCGGGTGACC 
GACATCATCA TGCAGAAAAT GACCATCTCC AACATGGAGG CGGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAACTCAC AAAAAGGCGA GAGAAACTTT CTAAAAGGAG AGAGAAGATA 
GTCAAGGAGA GCGGAGAGGG AGATAAAAGT GTGGCTAACA TCATCGAGGA GATGGAGTCC 
CTGACAGCCA ACATAGATTA CATCAATGAT AGCATTGCCG ACTGTCAAGC CAACATCATG 
CAGATGGAGG AGGCAAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATTAATGCC 
TGTACACTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTGCAGG CTGCCCAGAA AGAGGCTCAA ATTAAAGTCC TCGAGGGTCG ACTCAAACAG 
ACCGAAATCA CCAGTGCAAC CCAGAACCAA CTCTTATTCC ATATGCTGAA GGAGAAGGCA 
GAGCTAAACC CAGAGCTGGA TGCCTTGCTA GGCCACGCAC TGCAAGATCT AGATGGCGCC 
CCACCAGAAA ACGAGGAGGA CAGCAGCGAG GAAGATGGCC CTTTACACAG CCCGGGGTCG 
GAGGGAAGCA CGTTATCTTC AGACCTTATG AAGCTTTGTG GTGAAGTGAA ACCAAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAGATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGATGCCTCC TTGTCTGGCC CTCTGGCACC TGTTGCAGAA 
GGGCAGGAGA TTGGAATGAA CACAGAGACA AGTGGTACTT CTGCTAGGGA CAAAGAGCTT 
CTTGCCCCAT CTGGCTTACC TTCTAAGATA GGCAGCATTT CCAGACAGTC ATCTCTATCA 
GAAAAAAAAG TGCCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAGGCAGAT 
AAACCAAAGG CGAAGGAACA TAAACAAGGC ATAATCAACC CATTTCCTGC CTGCAAAGGA 
GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC ATAGCTGAAG GGCACACCAA GGCTGTGCTC 
TGTGTGGATT CTACGGATGA TCTTCTCTTC ACCGGATCAA AAGATCGCAC TTGTAAAGTA 
TGGAATCTCG TGACTGGACA GGAAATTATG TCCCTGGGGG TTCACCCTAA CAACGTGGTG 
TCTGTAAAAT ACTGTAATTA TACCAGTCTG GTCTTCACCG TGTCGACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCAAAATGC ATTCGAACAT TAACATCTTC AGGTCAAGTT 
ACCCTTGGAG AAGCGTGTTC TGCCAGCACC AGCCGGACAG TAGCTATTCC TTCCGGGGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACTT TCCTCTACGC GGCCTCTGGA 
AATGCCGTCA GAATGTGGGA CCTTAAAAGG TTTCAGTCTA CAGGAAAGCT AACCGGACAC 
CTGGGTCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ATGGACAGGA CCTCATCATC 
ACTGGCTCAA AAGACCACTA CATCAAAATG TTCGATGTGA CTGAAGGGGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT CGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGCGATA ACCTATTCAG TGGGTCCAGA GATAATGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT GCACACAAAG ACTGGGTGTG TGCCCTGGGC 
CTGGTGCCAG GCCATCCGGT TTTGCTGAGT GGCTGCAGAG GCGGCATTCT GAAACTCTGG 
AATGTGGACA CTTTTGTGCC CGTTGGAGAG ATGAGAGGTC ATGACAGTCC CATCAATGCC 
ATTTGTGTTA ACTCCACCCA TGTCTTTACT GCTGCTGATG ATCGAACCGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAACTC TCTGACACCG GCGATCTGGG GGAGGATATT 
GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316468.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171957.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.

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CloneID	OMu79034
Clone ID Related Accession (Same CDS sequence)	XM_017316468.1 , XM_017316468.2
Accession Version	XM_017316468.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171957.1
CDS	2461..7266
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316468.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316468.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316470.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171959.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.

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CloneID	OMu79035
Clone ID Related Accession (Same CDS sequence)	XM_017316470.1
Accession Version	XM_017316470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT GTCTGATGAA AGTGACTCCT CTTTGTCCGA GGTGCACAGA 
TCCACCAGAA GAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171959.1
CDS	2461..7257
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316470.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316470.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT GTCTGATGAA AGTGACTCCT CTTTGTCCGA GGTGCACAGA 
TCCACCAGAA GAGGCATAAT CAACCCATTT CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT 
CTTCAGTGCG TTCACATAGC TGAAGGGCAC ACCAAGGCTG TGCTCTGTGT GGATTCTACG 
GATGATCTTC TCTTCACCGG ATCAAAAGAT CGCACTTGTA AAGTATGGAA TCTCGTGACT 
GGACAGGAAA TTATGTCCCT GGGGGTTCAC CCTAACAACG TGGTGTCTGT AAAATACTGT 
AATTATACCA GTCTGGTCTT CACCGTGTCG ACATCTTATA TTAAGGTGTG GGATATCAGA 
GAGTCAGCAA AATGCATTCG AACATTAACA TCTTCAGGTC AAGTTACCCT TGGAGAAGCG 
TGTTCTGCCA GCACCAGCCG GACAGTAGCT ATTCCTTCCG GGGAGAGCCA GATCAATCAA 
ATTGCACTAA ACCCAACTGG CACTTTCCTC TACGCGGCCT CTGGAAATGC CGTCAGAATG 
TGGGACCTTA AAAGGTTTCA GTCTACAGGA AAGCTAACCG GACACCTGGG TCCTGTTATG 
TGCCTTACGG TAGACCAGAT CTCCAATGGA CAGGACCTCA TCATCACTGG CTCAAAAGAC 
CACTACATCA AAATGTTCGA TGTGACTGAA GGGGCTCTTG GAACTGTAAG TCCCACCCAC 
AACTTCGAGC CTCCTCATTA TGATGGGATA GAAGCACTGG CCATTCAAGG CGATAACCTA 
TTCAGTGGGT CCAGAGATAA TGGAATCAAG AAATGGGACT TAGCTCAGAA AGGTCTTCTT 
CAGCAAGTCC CAAATGCACA CAAAGACTGG GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT 
CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC ATTCTGAAAC TCTGGAATGT GGACACTTTT 
GTGCCCGTTG GAGAGATGAG AGGTCATGAC AGTCCCATCA ATGCCATTTG TGTTAACTCC 
ACCCATGTCT TTACTGCTGC TGATGATCGA ACCGTGAGAA TCTGGAAGGC CCACAACTTG 
CAAGATGGTC AACTCTCTGA CACCGGCGAT CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316471.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171960.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.

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CloneID	OMu79036
Clone ID Related Accession (Same CDS sequence)	XM_017316471.1 , XM_017316471.2
Accession Version	XM_017316471.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT GTCTGATGAA AGTGACTCCT CTTTGTCCGA GGTGCACAGA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171960.1
CDS	2461..7245
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316471.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316471.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT GTCTGATGAA AGTGACTCCT CTTTGTCCGA GGTGCACAGA 
GGCATAATCA ACCCATTTCC TGCCTGCAAA GGAGTCAGAG CCTCTCCTCT TCAGTGCGTT 
CACATAGCTG AAGGGCACAC CAAGGCTGTG CTCTGTGTGG ATTCTACGGA TGATCTTCTC 
TTCACCGGAT CAAAAGATCG CACTTGTAAA GTATGGAATC TCGTGACTGG ACAGGAAATT 
ATGTCCCTGG GGGTTCACCC TAACAACGTG GTGTCTGTAA AATACTGTAA TTATACCAGT 
CTGGTCTTCA CCGTGTCGAC ATCTTATATT AAGGTGTGGG ATATCAGAGA GTCAGCAAAA 
TGCATTCGAA CATTAACATC TTCAGGTCAA GTTACCCTTG GAGAAGCGTG TTCTGCCAGC 
ACCAGCCGGA CAGTAGCTAT TCCTTCCGGG GAGAGCCAGA TCAATCAAAT TGCACTAAAC 
CCAACTGGCA CTTTCCTCTA CGCGGCCTCT GGAAATGCCG TCAGAATGTG GGACCTTAAA 
AGGTTTCAGT CTACAGGAAA GCTAACCGGA CACCTGGGTC CTGTTATGTG CCTTACGGTA 
GACCAGATCT CCAATGGACA GGACCTCATC ATCACTGGCT CAAAAGACCA CTACATCAAA 
ATGTTCGATG TGACTGAAGG GGCTCTTGGA ACTGTAAGTC CCACCCACAA CTTCGAGCCT 
CCTCATTATG ATGGGATAGA AGCACTGGCC ATTCAAGGCG ATAACCTATT CAGTGGGTCC 
AGAGATAATG GAATCAAGAA ATGGGACTTA GCTCAGAAAG GTCTTCTTCA GCAAGTCCCA 
AATGCACACA AAGACTGGGT GTGTGCCCTG GGCCTGGTGC CAGGCCATCC GGTTTTGCTG 
AGTGGCTGCA GAGGCGGCAT TCTGAAACTC TGGAATGTGG ACACTTTTGT GCCCGTTGGA 
GAGATGAGAG GTCATGACAG TCCCATCAAT GCCATTTGTG TTAACTCCAC CCATGTCTTT 
ACTGCTGCTG ATGATCGAAC CGTGAGAATC TGGAAGGCCC ACAACTTGCA AGATGGTCAA 
CTCTCTGACA CCGGCGATCT GGGGGAGGAT ATTGCCAGTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316472.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171961.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.

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CloneID	OMu79037
Clone ID Related Accession (Same CDS sequence)	XM_017316472.1
Accession Version	XM_017316472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GCAGATCCAC CAGAAGAGGC 
ATAATCAACC CATTTCCTGC CTGCAAAGGA GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC 
ATAGCTGAAG GGCACACCAA GGCTGTGCTC TGTGTGGATT CTACGGATGA TCTTCTCTTC 
ACCGGATCAA AAGATCGCAC TTGTAAAGTA TGGAATCTCG TGACTGGACA GGAAATTATG 
TCCCTGGGGG TTCACCCTAA CAACGTGGTG TCTGTAAAAT ACTGTAATTA TACCAGTCTG 
GTCTTCACCG TGTCGACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCAAAATGC 
ATTCGAACAT TAACATCTTC AGGTCAAGTT ACCCTTGGAG AAGCGTGTTC TGCCAGCACC 
AGCCGGACAG TAGCTATTCC TTCCGGGGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACTT TCCTCTACGC GGCCTCTGGA AATGCCGTCA GAATGTGGGA CCTTAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACCGGACAC CTGGGTCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ATGGACAGGA CCTCATCATC ACTGGCTCAA AAGACCACTA CATCAAAATG 
TTCGATGTGA CTGAAGGGGC TCTTGGAACT GTAAGTCCCA CCCACAACTT CGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGCGATA ACCTATTCAG TGGGTCCAGA 
GATAATGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT 
GCACACAAAG ACTGGGTGTG TGCCCTGGGC CTGGTGCCAG GCCATCCGGT TTTGCTGAGT 
GGCTGCAGAG GCGGCATTCT GAAACTCTGG AATGTGGACA CTTTTGTGCC CGTTGGAGAG 
ATGAGAGGTC ATGACAGTCC CATCAATGCC ATTTGTGTTA ACTCCACCCA TGTCTTTACT 
GCTGCTGATG ATCGAACCGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAACTC 
TCTGACACCG GCGATCTGGG GGAGGATATT GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171961.1
CDS	2461..7242
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316472.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316472.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GCAGATCCAC CAGAAGAGGC 
ATAATCAACC CATTTCCTGC CTGCAAAGGA GTCAGAGCCT CTCCTCTTCA GTGCGTTCAC 
ATAGCTGAAG GGCACACCAA GGCTGTGCTC TGTGTGGATT CTACGGATGA TCTTCTCTTC 
ACCGGATCAA AAGATCGCAC TTGTAAAGTA TGGAATCTCG TGACTGGACA GGAAATTATG 
TCCCTGGGGG TTCACCCTAA CAACGTGGTG TCTGTAAAAT ACTGTAATTA TACCAGTCTG 
GTCTTCACCG TGTCGACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCAAAATGC 
ATTCGAACAT TAACATCTTC AGGTCAAGTT ACCCTTGGAG AAGCGTGTTC TGCCAGCACC 
AGCCGGACAG TAGCTATTCC TTCCGGGGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACTT TCCTCTACGC GGCCTCTGGA AATGCCGTCA GAATGTGGGA CCTTAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACCGGACAC CTGGGTCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ATGGACAGGA CCTCATCATC ACTGGCTCAA AAGACCACTA CATCAAAATG 
TTCGATGTGA CTGAAGGGGC TCTTGGAACT GTAAGTCCCA CCCACAACTT CGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGCGATA ACCTATTCAG TGGGTCCAGA 
GATAATGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGTC TTCTTCAGCA AGTCCCAAAT 
GCACACAAAG ACTGGGTGTG TGCCCTGGGC CTGGTGCCAG GCCATCCGGT TTTGCTGAGT 
GGCTGCAGAG GCGGCATTCT GAAACTCTGG AATGTGGACA CTTTTGTGCC CGTTGGAGAG 
ATGAGAGGTC ATGACAGTCC CATCAATGCC ATTTGTGTTA ACTCCACCCA TGTCTTTACT 
GCTGCTGATG ATCGAACCGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAACTC 
TCTGACACCG GCGATCTGGG GGAGGATATT GCCAGTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316473.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171962.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu79038
Clone ID Related Accession (Same CDS sequence)	XM_017316473.1
Accession Version	XM_017316473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GATCCACCAG AAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171962.1
CDS	2461..7239
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316473.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316473.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GATCCACCAG AAGAGGCATA 
ATCAACCCAT TTCCTGCCTG CAAAGGAGTC AGAGCCTCTC CTCTTCAGTG CGTTCACATA 
GCTGAAGGGC ACACCAAGGC TGTGCTCTGT GTGGATTCTA CGGATGATCT TCTCTTCACC 
GGATCAAAAG ATCGCACTTG TAAAGTATGG AATCTCGTGA CTGGACAGGA AATTATGTCC 
CTGGGGGTTC ACCCTAACAA CGTGGTGTCT GTAAAATACT GTAATTATAC CAGTCTGGTC 
TTCACCGTGT CGACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC AAAATGCATT 
CGAACATTAA CATCTTCAGG TCAAGTTACC CTTGGAGAAG CGTGTTCTGC CAGCACCAGC 
CGGACAGTAG CTATTCCTTC CGGGGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACTTTCC TCTACGCGGC CTCTGGAAAT GCCGTCAGAA TGTGGGACCT TAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC CGGACACCTG GGTCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAATG GACAGGACCT CATCATCACT GGCTCAAAAG ACCACTACAT CAAAATGTTC 
GATGTGACTG AAGGGGCTCT TGGAACTGTA AGTCCCACCC ACAACTTCGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGCGATAACC TATTCAGTGG GTCCAGAGAT 
AATGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGTCTTC TTCAGCAAGT CCCAAATGCA 
CACAAAGACT GGGTGTGTGC CCTGGGCCTG GTGCCAGGCC ATCCGGTTTT GCTGAGTGGC 
TGCAGAGGCG GCATTCTGAA ACTCTGGAAT GTGGACACTT TTGTGCCCGT TGGAGAGATG 
AGAGGTCATG ACAGTCCCAT CAATGCCATT TGTGTTAACT CCACCCATGT CTTTACTGCT 
GCTGATGATC GAACCGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAACTCTCT 
GACACCGGCG ATCTGGGGGA GGATATTGCC AGTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316474.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171963.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OMu79039
Clone ID Related Accession (Same CDS sequence)	XM_017316474.2 , XM_017316474.1
Accession Version	XM_017316474.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171963.1
CDS	2461..7227
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316474.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316474.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATGTCTGATG AAAGTGACTC CTCTTTGTCC GAGGTGCACA GAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316475.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171964.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X35, mRNA.

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CloneID	OMu79040
Clone ID Related Accession (Same CDS sequence)	XM_017316475.2 , XM_017316475.1
Accession Version	XM_017316475.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171964.1
CDS	2461..7227
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316475.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316475.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA TCTAGATGGC 
GCCCCACCAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TAGGCATAAT CAACCCATTT 
CCTGCCTGCA AAGGAGTCAG AGCCTCTCCT CTTCAGTGCG TTCACATAGC TGAAGGGCAC 
ACCAAGGCTG TGCTCTGTGT GGATTCTACG GATGATCTTC TCTTCACCGG ATCAAAAGAT 
CGCACTTGTA AAGTATGGAA TCTCGTGACT GGACAGGAAA TTATGTCCCT GGGGGTTCAC 
CCTAACAACG TGGTGTCTGT AAAATACTGT AATTATACCA GTCTGGTCTT CACCGTGTCG 
ACATCTTATA TTAAGGTGTG GGATATCAGA GAGTCAGCAA AATGCATTCG AACATTAACA 
TCTTCAGGTC AAGTTACCCT TGGAGAAGCG TGTTCTGCCA GCACCAGCCG GACAGTAGCT 
ATTCCTTCCG GGGAGAGCCA GATCAATCAA ATTGCACTAA ACCCAACTGG CACTTTCCTC 
TACGCGGCCT CTGGAAATGC CGTCAGAATG TGGGACCTTA AAAGGTTTCA GTCTACAGGA 
AAGCTAACCG GACACCTGGG TCCTGTTATG TGCCTTACGG TAGACCAGAT CTCCAATGGA 
CAGGACCTCA TCATCACTGG CTCAAAAGAC CACTACATCA AAATGTTCGA TGTGACTGAA 
GGGGCTCTTG GAACTGTAAG TCCCACCCAC AACTTCGAGC CTCCTCATTA TGATGGGATA 
GAAGCACTGG CCATTCAAGG CGATAACCTA TTCAGTGGGT CCAGAGATAA TGGAATCAAG 
AAATGGGACT TAGCTCAGAA AGGTCTTCTT CAGCAAGTCC CAAATGCACA CAAAGACTGG 
GTGTGTGCCC TGGGCCTGGT GCCAGGCCAT CCGGTTTTGC TGAGTGGCTG CAGAGGCGGC 
ATTCTGAAAC TCTGGAATGT GGACACTTTT GTGCCCGTTG GAGAGATGAG AGGTCATGAC 
AGTCCCATCA ATGCCATTTG TGTTAACTCC ACCCATGTCT TTACTGCTGC TGATGATCGA 
ACCGTGAGAA TCTGGAAGGC CCACAACTTG CAAGATGGTC AACTCTCTGA CACCGGCGAT 
CTGGGGGAGG ATATTGCCAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316476.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171965.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X36, mRNA.

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CloneID	OMu79041
Clone ID Related Accession (Same CDS sequence)	XM_017316476.1 , XM_017316476.2
Accession Version	XM_017316476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171965.1
CDS	2461..7206
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSMSDESDSSLSEVHRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316476.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316476.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG AAAACGAGGA GGACAGCAGC GAGGAAGATG GCCCTTTACA CAGCCCGGGG 
TCGGAGGGAA GCACGTTATC TTCAGACCTT ATGAAGCTTT GTGGTGAAGT GAAACCAAAG 
AACAAGGCTC GAAGGAGAAC CACCACTCAG ATGGAGTTGC TGTATGCAGA TAGCAGTGAA 
GTAGCCTCAG ACACTAGTGC AGGAGATGCC TCCTTGTCTG GCCCTCTGGC ACCTGTTGCA 
GAAGGGCAGG AGATTGGAAT GAACACAGAG ACAAGTGGTA CTTCTGCTAG GGACAAAGAG 
CTTCTTGCCC CATCTGGCTT ACCTTCTAAG ATAGGCAGCA TGTCTGATGA AAGTGACTCC 
TCTTTGTCCG AGGTGCACAG AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316477.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171966.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X37, mRNA.

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CloneID	OMu79042
Clone ID Related Accession (Same CDS sequence)	XM_017316477.2 , XM_017316477.1
Accession Version	XM_017316477.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171966.1
CDS	2461..7206
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316477.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316477.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGGCT ACAGAAACTT GAGGAAAGCG GTCGAGAAGA AAGAAGTGTT 
GCCGGGAAAG ACGATAATGC AGACACTGAC CAGGAGAAGA AAGAAGAAAA GGGTGTTTCA 
GAGAAAGAAA ACAATGAGCT AGACGTGGAA GAGAATCAAG AAGTGAGTGA CCACGAGGAT 
GAGGAAGAGG AGGAAGAGGA CGAGGAGGAA GAGGATGACA TTGAAGGAGA AGAAAGCTCT 
GATGAATCAG ATTCCGAATC TGATGAAAAA GCTAACTATC AAGCCGACTT AGCAAATATC 
ACCTGCGAGA TTGCGATTAA GCAGAAGCTG ATCGACGAAC TGGAGAACAG CCAGAAACGG 
CTGCAGACCC TGAAAAAGCA GTACGAGGAG AAGCTGATGA TGCTCCAACA TAAGATCCGG 
GACACGCAGC TGGAGAGGGA CCAGGTGCTC CAGAACCTAG GCTCGGTGGA GTCATACTCG 
GAAGAAAAGG CCAAGAAAGT GAAGTGCGAA TATGAGAAGA AGCTCCACGC CATGAACAAA 
GAGCTGCAGC GACTGCAGAC GGCCCAAAAG GAGCACGCCA GGCTCCTCAA AAACCAGTCT 
CAGTATGAAA AGCAGCTCAA GAAACTGCAG CAAGACGTCA TGGAAATGAA GAAAACCAAG 
GTTCGTCTAA TGAAGCAGAT GAAAGAAGAG CAGGAGAAAG CCCGGCTGAC AGAATCTCGA 
AGGAACCGGG AAATCGCTCA GCTGAAGAAG GATCAGCGCA AGCGAGATCA TCAACTTAGA 
CTTCTAGAGG CCCAGAAAAG AAATCAAGAA GTAGTTCTGC GACGCAAAAC GGAAGAGGTT 
ACAGCTCTCC GCAGGCAAGT GAGGCCCATG TCTGATAAAG TAGCGGGAAA AGTTACTCGG 
AAGCTGAGCT CATCCGAAAG CCCCGCTCCG GACACAGGTT CCAGTGCGGC TTCCGGGGAA 
GCAGACACAT CACGGCCAGG CACCCAGCAG AAAATGAGGA TCCCCGTGGC AAGAGTCCAG 
GCATTACCAA CACCTACAAC AAATGGCACC AGGAAAAAAT ATCAGAGGAA AGGATTCACT 
GGCCGGGTGT TCACTTCCAA GACAGCCCGC ATGAAGTGGC AGCTACTGGA GCGCCGGGTG 
ACCGACATCA TCATGCAGAA AATGACCATC TCCAACATGG AGGCGGACAT GAACAGACTC 
CTCAGGCAAC GGGAAGAACT CACAAAAAGG CGAGAGAAAC TTTCTAAAAG GAGAGAGAAG 
ATAGTCAAGG AGAGCGGAGA GGGAGATAAA AGTGTGGCTA ACATCATCGA GGAGATGGAG 
TCCCTGACAG CCAACATAGA TTACATCAAT GATAGCATTG CCGACTGTCA AGCCAACATC 
ATGCAGATGG AGGAGGCAAA GGAAGAAGGG GAGACATTGG ATGTCACCGC TGTCATTAAT 
GCCTGTACAC TGACAGAAGC TCGGTACCTG CTAGATCACT TCCTGTCAAT GGGCATCAAT 
AAGGGTCTGC AGGCTGCCCA GAAAGAGGCT CAAATTAAAG TCCTCGAGGG TCGACTCAAA 
CAGACCGAAA TCACCAGTGC AACCCAGAAC CAACTCTTAT TCCATATGCT GAAGGAGAAG 
GCAGAGCTAA ACCCAGAGCT GGATGCCTTG CTAGGCCACG CACTGCAAGA AAACGAGGAG 
GACAGCAGCG AGGAAGATGG CCCTTTACAC AGCCCGGGGT CGGAGGGAAG CACGTTATCT 
TCAGACCTTA TGAAGCTTTG TGGTGAAGTG AAACCAAAGA ACAAGGCTCG AAGGAGAACC 
ACCACTCAGA TGGAGTTGCT GTATGCAGAT AGCAGTGAAG TAGCCTCAGA CACTAGTGCA 
GGAGATGCCT CCTTGTCTGG CCCTCTGGCA CCTGTTGCAG AAGGGCAGGA GATTGGAATG 
AACACAGAGA CAAGTGGTAC TTCTGCTAGG GACAAAGAGC TTCTTGCCCC ATCTGGCTTA 
CCTTCTAAGA TAGGCAGCAT AGGCATAATC AACCCATTTC CTGCCTGCAA AGGAGTCAGA 
GCCTCTCCTC TTCAGTGCGT TCACATAGCT GAAGGGCACA CCAAGGCTGT GCTCTGTGTG 
GATTCTACGG ATGATCTTCT CTTCACCGGA TCAAAAGATC GCACTTGTAA AGTATGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCCCTG GGGGTTCACC CTAACAACGT GGTGTCTGTA 
AAATACTGTA ATTATACCAG TCTGGTCTTC ACCGTGTCGA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCAAA ATGCATTCGA ACATTAACAT CTTCAGGTCA AGTTACCCTT 
GGAGAAGCGT GTTCTGCCAG CACCAGCCGG ACAGTAGCTA TTCCTTCCGG GGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACTTTCCTCT ACGCGGCCTC TGGAAATGCC 
GTCAGAATGT GGGACCTTAA AAGGTTTCAG TCTACAGGAA AGCTAACCGG ACACCTGGGT 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAATGGAC AGGACCTCAT CATCACTGGC 
TCAAAAGACC ACTACATCAA AATGTTCGAT GTGACTGAAG GGGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTCGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGC 
GATAACCTAT TCAGTGGGTC CAGAGATAAT GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGTCTTCTTC AGCAAGTCCC AAATGCACAC AAAGACTGGG TGTGTGCCCT GGGCCTGGTG 
CCAGGCCATC CGGTTTTGCT GAGTGGCTGC AGAGGCGGCA TTCTGAAACT CTGGAATGTG 
GACACTTTTG TGCCCGTTGG AGAGATGAGA GGTCATGACA GTCCCATCAA TGCCATTTGT 
GTTAACTCCA CCCATGTCTT TACTGCTGCT GATGATCGAA CCGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA ACTCTCTGAC ACCGGCGATC TGGGGGAGGA TATTGCCAGT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316478.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171967.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X38, mRNA.

Price & Availability↑

CloneID	OMu79043
Clone ID Related Accession (Same CDS sequence)	XM_017316478.1 , XM_017316478.2
Accession Version	XM_017316478.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACCG GATTTGACGT GAACATCATG GAGGAAGAGC AGGGCATCAT CTCTCGTGCT 
GTTAGACACC TGTTCAAGAG TATTGATGAG AAAAAGACCT CAGCGATTAA AAACGGGCTG 
CCCCCTCCTG AATTCAAAGT GAATGCCCAG TTCCTAGAGC TCTATAATGA AGAGGTCCTT 
GACTTGTTTG ATACCACTCG GGATATTGAT GCAAAAAATA AAAAATCAAA TATAAGAATT 
CATGAAGATT CAACTGGAGG AATTTATACT GTGGGCGTCA CAACACGCAC TGTGAATACA 
GAACCGGAGA TGATGCAGTG TCTGAAGCTG GGCGCTCTCT CGCGCACCAC GGCCAGCACC 
CAGATGAACG TACAGAGCTC TCGTTCACAC GCCATCTTTA CCATTCACGT GTGTCAAACC 
AGAGTGTGTC CCCAAACAGA TGCTGAGAAC GCAACTGATA ATAAGCTGAT CTCCGAATCG 
TCGCCAATGA ATGAGTTTGA GACGCTGACG GCGAAGTTTC ACTTTGTTGA TCTGGCGGGA 
TCGGAAAGAC TGAAGAGAAC CGGAGCTACA GGCGAGAGAG CAAAAGAGGG CATTTCCATC 
AACTGCGGGC TTTTGGCTCT TGGGAATGTA ATCAGCGCCT TGGGGGACAA GAGCAAGAGA 
GCCACCCATG TCCCTTACAG AGACTCTAAG CTGACAAGAC TCTTGCAGGA TTCCCTTGGG 
GGTAACAGCC AAACCATCAT GATAGCATGC GTCAGCCCTT CAGACAGGGA TTTCATGGAG 
ACGTTAAACA CTCTGAAGTA CGCCAATCGA GCGAGAAATA TCAAGAACAA GGTGATGGTC 
AATCAAGACA GAGCCAGTCA GCAAATCAAC GCGCTGCGGA GCGAGATCAC GCGCCTTCAG 
ATGGAGCTCA TGGAATACAA AACCGGTAAA AGAATAATTG ACGAGGAAGG CGTGGAAAGC 
ATCAATGACA TGTTTCATGA GAATGCTATG CTGCAGACGG AAAATAATAA TCTGCGTGTA 
AGAATAAAAG CCATGCAGGA GACCGTTGAT GCACTGAGGG CCAGAATCAC GCAGCTTGTC 
AGTGAGCAGG CCAACCAAGT TCTTGCCCGA GCAGGTGAAG GGAACGAAGA GATCAGTAAT 
ATGATTCATA GTTATATCAA AGAAATTGAA GACCTCAGGG CAAAATTATT AGAAAGTGAA 
GCAGTGAACG AGAACCTTCG GAAGAACTTG ACCAGAGCCA CGGCGAGATC TCCTTACTTC 
AGTGCCTCCT CAGCTTTCTC GCCTACTATA CTGTCTTCAG ACAAGGAGAC TATCGAAATT 
ATAGATCTAG CAAAGAAAGA CTTGGAGAAG CTAAAACGGA AAGAGAAGAA GAAGAAGAAA 
AGGCTACAGA AACTTGAGGA AAGCGGTCGA GAAGAAAGAA GTGTTGCCGG GAAAGACGAT 
AATGCAGACA CTGACCAGGA GAAGAAAGAA GAAAAGGGTG TTTCAGAGAA AGAAAACAAT 
GAGCTAGACG TGGAAGAGAA TCAAGAAGTG AGTGACCACG AGGATGAGGA AGAGGAGGAA 
GAGGACGAGG AGGAAGAGGA TGACATTGAA GGAGAAGAAA GCTCTGATGA ATCAGATTCC 
GAATCTGATG AAAAAGCTAA CTATCAAGCC GACTTAGCAA ATATCACCTG CGAGATTGCG 
ATTAAGCAGA AGCTGATCGA CGAACTGGAG AACAGCCAGA AACGGCTGCA GACCCTGAAA 
AAGCAGTACG AGGAGAAGCT GATGATGCTC CAACATAAGA TCCGGGACAC GCAGCTGGAG 
AGGGACCAGG TGCTCCAGAA CCTAGGCTCG GTGGAGTCAT ACTCGGAAGA AAAGGCCAAG 
AAAGTGAAGT GCGAATATGA GAAGAAGCTC CACGCCATGA ACAAAGAGCT GCAGCGACTG 
CAGACGGCCC AAAAGGAGCA CGCCAGGCTC CTCAAAAACC AGTCTCAGTA TGAAAAGCAG 
CTCAAGAAAC TGCAGCAAGA CGTCATGGAA ATGAAGAAAA CCAAGGTTCG TCTAATGAAG 
CAGATGAAAG AAGAGCAGGA GAAAGCCCGG CTGACAGAAT CTCGAAGGAA CCGGGAAATC 
GCTCAGCTGA AGAAGGATCA GCGCAAGCGA GATCATCAAC TTAGACTTCT AGAGGCCCAG 
AAAAGAAATC AAGAAGTAGT TCTGCGACGC AAAACGGAAG AGGTTACAGC TCTCCGCAGG 
CAAGTGAGGC CCATGTCTGA TAAAGTAGCG GGAAAAGTTA CTCGGAAGCT GAGCTCATCC 
GAAAGCCCCG CTCCGGACAC AGGTTCCAGT GCGGCTTCCG GGGAAGCAGA CACATCACGG 
CCAGGCACCC AGCAGAAAAT GAGGATCCCC GTGGCAAGAG TCCAGGCATT ACCAACACCT 
ACAACAAATG GCACCAGGAA AAAATATCAG AGGAAAGGAT TCACTGGCCG GGTGTTCACT 
TCCAAGACAG CCCGCATGAA GTGGCAGCTA CTGGAGCGCC GGGTGACCGA CATCATCATG 
CAGAAAATGA CCATCTCCAA CATGGAGGCG GACATGAACA GACTCCTCAG GCAACGGGAA 
GAACTCACAA AAAGGCGAGA GAAACTTTCT AAAAGGAGAG AGAAGATAGT CAAGGAGAGC 
GGAGAGGGAG ATAAAAGTGT GGCTAACATC ATCGAGGAGA TGGAGTCCCT GACAGCCAAC 
ATAGATTACA TCAATGATAG CATTGCCGAC TGTCAAGCCA ACATCATGCA GATGGAGGAG 
GCAAAGGAAG AAGGGGAGAC ATTGGATGTC ACCGCTGTCA TTAATGCCTG TACACTGACA 
GAAGCTCGGT ACCTGCTAGA TCACTTCCTG TCAATGGGCA TCAATAAGGG TCTGCAGGCT 
GCCCAGAAAG AGGCTCAAAT TAAAGTCCTC GAGGGTCGAC TCAAACAGAC CGAAATCACC 
AGTGCAACCC AGAACCAACT CTTATTCCAT ATGCTGAAGG AGAAGGCAGA GCTAAACCCA 
GAGCTGGATG CCTTGCTAGG CCACGCACTG CAAGATCTAG ATGGCGCCCC ACCAGAAAAC 
GAGGAGGACA GCAGCGAGGA AGATGGCCCT TTACACAGCC CGGGGTCGGA GGGAAGCACG 
TTATCTTCAG ACCTTATGAA GCTTTGTGGT GAAGTGAAAC CAAAGAACAA GGCTCGAAGG 
AGAACCACCA CTCAGATGGA GTTGCTGTAT GCAGATAGCA GTGAAGTAGC CTCAGACACT 
AGTGCAGGAG ATGCCTCCTT GTCTGGCCCT CTGGCACCTG TTGCAGAAGG GCAGGAGATT 
GGAATGAACA CAGAGACAAG TGGTACTTCT GCTAGGGACA AAGAGCTTCT TGCCCCATCT 
GGCTTACCTT CTAAGATAGG CAGCATTTCC AGACAGTCAT CTCTATCAGA AAAAAAAGTG 
CCAGAGCCTT CCCCTGTAAC AAGGAGAAAG GCATATGAGA AGGCAGATAA ACCAAAGGCG 
AAGGAACATA AACACTCAGA TTCGGGCGCT TCCGAGACTA GTCTCTCACC TCCTTCCTCC 
CCACCAAGCC GGCCCCGTAA CGAACTGAAT GTGTTTAATC GCCTTACTGT GCCTCAGGGA 
ACCCCGTCAG TTCAGCAGGA TAAGTCTGAT GAAAGTGACT CCTCTTTGTC CGAGGTGCAC 
AGCAGATCCA CCAGAAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171967.1
CDS	380..5122
Translation	MGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESGREERSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSISRQSSLSEKKVPEPSPVTRRKAYEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316478.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316478.1

ORF Insert Sequence:

ATGGGAACCG GATTTGACGT GAACATCATG GAGGAAGAGC AGGGCATCAT CTCTCGTGCT 
GTTAGACACC TGTTCAAGAG TATTGATGAG AAAAAGACCT CAGCGATTAA AAACGGGCTG 
CCCCCTCCTG AATTCAAAGT GAATGCCCAG TTCCTAGAGC TCTATAATGA AGAGGTCCTT 
GACTTGTTTG ATACCACTCG GGATATTGAT GCAAAAAATA AAAAATCAAA TATAAGAATT 
CATGAAGATT CAACTGGAGG AATTTATACT GTGGGCGTCA CAACACGCAC TGTGAATACA 
GAACCGGAGA TGATGCAGTG TCTGAAGCTG GGCGCTCTCT CGCGCACCAC GGCCAGCACC 
CAGATGAACG TACAGAGCTC TCGTTCACAC GCCATCTTTA CCATTCACGT GTGTCAAACC 
AGAGTGTGTC CCCAAACAGA TGCTGAGAAC GCAACTGATA ATAAGCTGAT CTCCGAATCG 
TCGCCAATGA ATGAGTTTGA GACGCTGACG GCGAAGTTTC ACTTTGTTGA TCTGGCGGGA 
TCGGAAAGAC TGAAGAGAAC CGGAGCTACA GGCGAGAGAG CAAAAGAGGG CATTTCCATC 
AACTGCGGGC TTTTGGCTCT TGGGAATGTA ATCAGCGCCT TGGGGGACAA GAGCAAGAGA 
GCCACCCATG TCCCTTACAG AGACTCTAAG CTGACAAGAC TCTTGCAGGA TTCCCTTGGG 
GGTAACAGCC AAACCATCAT GATAGCATGC GTCAGCCCTT CAGACAGGGA TTTCATGGAG 
ACGTTAAACA CTCTGAAGTA CGCCAATCGA GCGAGAAATA TCAAGAACAA GGTGATGGTC 
AATCAAGACA GAGCCAGTCA GCAAATCAAC GCGCTGCGGA GCGAGATCAC GCGCCTTCAG 
ATGGAGCTCA TGGAATACAA AACCGGTAAA AGAATAATTG ACGAGGAAGG CGTGGAAAGC 
ATCAATGACA TGTTTCATGA GAATGCTATG CTGCAGACGG AAAATAATAA TCTGCGTGTA 
AGAATAAAAG CCATGCAGGA GACCGTTGAT GCACTGAGGG CCAGAATCAC GCAGCTTGTC 
AGTGAGCAGG CCAACCAAGT TCTTGCCCGA GCAGGTGAAG GGAACGAAGA GATCAGTAAT 
ATGATTCATA GTTATATCAA AGAAATTGAA GACCTCAGGG CAAAATTATT AGAAAGTGAA 
GCAGTGAACG AGAACCTTCG GAAGAACTTG ACCAGAGCCA CGGCGAGATC TCCTTACTTC 
AGTGCCTCCT CAGCTTTCTC GCCTACTATA CTGTCTTCAG ACAAGGAGAC TATCGAAATT 
ATAGATCTAG CAAAGAAAGA CTTGGAGAAG CTAAAACGGA AAGAGAAGAA GAAGAAGAAA 
AGGCTACAGA AACTTGAGGA AAGCGGTCGA GAAGAAAGAA GTGTTGCCGG GAAAGACGAT 
AATGCAGACA CTGACCAGGA GAAGAAAGAA GAAAAGGGTG TTTCAGAGAA AGAAAACAAT 
GAGCTAGACG TGGAAGAGAA TCAAGAAGTG AGTGACCACG AGGATGAGGA AGAGGAGGAA 
GAGGACGAGG AGGAAGAGGA TGACATTGAA GGAGAAGAAA GCTCTGATGA ATCAGATTCC 
GAATCTGATG AAAAAGCTAA CTATCAAGCC GACTTAGCAA ATATCACCTG CGAGATTGCG 
ATTAAGCAGA AGCTGATCGA CGAACTGGAG AACAGCCAGA AACGGCTGCA GACCCTGAAA 
AAGCAGTACG AGGAGAAGCT GATGATGCTC CAACATAAGA TCCGGGACAC GCAGCTGGAG 
AGGGACCAGG TGCTCCAGAA CCTAGGCTCG GTGGAGTCAT ACTCGGAAGA AAAGGCCAAG 
AAAGTGAAGT GCGAATATGA GAAGAAGCTC CACGCCATGA ACAAAGAGCT GCAGCGACTG 
CAGACGGCCC AAAAGGAGCA CGCCAGGCTC CTCAAAAACC AGTCTCAGTA TGAAAAGCAG 
CTCAAGAAAC TGCAGCAAGA CGTCATGGAA ATGAAGAAAA CCAAGGTTCG TCTAATGAAG 
CAGATGAAAG AAGAGCAGGA GAAAGCCCGG CTGACAGAAT CTCGAAGGAA CCGGGAAATC 
GCTCAGCTGA AGAAGGATCA GCGCAAGCGA GATCATCAAC TTAGACTTCT AGAGGCCCAG 
AAAAGAAATC AAGAAGTAGT TCTGCGACGC AAAACGGAAG AGGTTACAGC TCTCCGCAGG 
CAAGTGAGGC CCATGTCTGA TAAAGTAGCG GGAAAAGTTA CTCGGAAGCT GAGCTCATCC 
GAAAGCCCCG CTCCGGACAC AGGTTCCAGT GCGGCTTCCG GGGAAGCAGA CACATCACGG 
CCAGGCACCC AGCAGAAAAT GAGGATCCCC GTGGCAAGAG TCCAGGCATT ACCAACACCT 
ACAACAAATG GCACCAGGAA AAAATATCAG AGGAAAGGAT TCACTGGCCG GGTGTTCACT 
TCCAAGACAG CCCGCATGAA GTGGCAGCTA CTGGAGCGCC GGGTGACCGA CATCATCATG 
CAGAAAATGA CCATCTCCAA CATGGAGGCG GACATGAACA GACTCCTCAG GCAACGGGAA 
GAACTCACAA AAAGGCGAGA GAAACTTTCT AAAAGGAGAG AGAAGATAGT CAAGGAGAGC 
GGAGAGGGAG ATAAAAGTGT GGCTAACATC ATCGAGGAGA TGGAGTCCCT GACAGCCAAC 
ATAGATTACA TCAATGATAG CATTGCCGAC TGTCAAGCCA ACATCATGCA GATGGAGGAG 
GCAAAGGAAG AAGGGGAGAC ATTGGATGTC ACCGCTGTCA TTAATGCCTG TACACTGACA 
GAAGCTCGGT ACCTGCTAGA TCACTTCCTG TCAATGGGCA TCAATAAGGG TCTGCAGGCT 
GCCCAGAAAG AGGCTCAAAT TAAAGTCCTC GAGGGTCGAC TCAAACAGAC CGAAATCACC 
AGTGCAACCC AGAACCAACT CTTATTCCAT ATGCTGAAGG AGAAGGCAGA GCTAAACCCA 
GAGCTGGATG CCTTGCTAGG CCACGCACTG CAAGATCTAG ATGGCGCCCC ACCAGAAAAC 
GAGGAGGACA GCAGCGAGGA AGATGGCCCT TTACACAGCC CGGGGTCGGA GGGAAGCACG 
TTATCTTCAG ACCTTATGAA GCTTTGTGGT GAAGTGAAAC CAAAGAACAA GGCTCGAAGG 
AGAACCACCA CTCAGATGGA GTTGCTGTAT GCAGATAGCA GTGAAGTAGC CTCAGACACT 
AGTGCAGGAG ATGCCTCCTT GTCTGGCCCT CTGGCACCTG TTGCAGAAGG GCAGGAGATT 
GGAATGAACA CAGAGACAAG TGGTACTTCT GCTAGGGACA AAGAGCTTCT TGCCCCATCT 
GGCTTACCTT CTAAGATAGG CAGCATTTCC AGACAGTCAT CTCTATCAGA AAAAAAAGTG 
CCAGAGCCTT CCCCTGTAAC AAGGAGAAAG GCATATGAGA AGGCAGATAA ACCAAAGGCG 
AAGGAACATA AACACTCAGA TTCGGGCGCT TCCGAGACTA GTCTCTCACC TCCTTCCTCC 
CCACCAAGCC GGCCCCGTAA CGAACTGAAT GTGTTTAATC GCCTTACTGT GCCTCAGGGA 
ACCCCGTCAG TTCAGCAGGA TAAGTCTGAT GAAAGTGACT CCTCTTTGTC CGAGGTGCAC 
AGCAGATCCA CCAGAAGAGG CATAATCAAC CCATTTCCTG CCTGCAAAGG AGTCAGAGCC 
TCTCCTCTTC AGTGCGTTCA CATAGCTGAA GGGCACACCA AGGCTGTGCT CTGTGTGGAT 
TCTACGGATG ATCTTCTCTT CACCGGATCA AAAGATCGCA CTTGTAAAGT ATGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCCCTGGGG GTTCACCCTA ACAACGTGGT GTCTGTAAAA 
TACTGTAATT ATACCAGTCT GGTCTTCACC GTGTCGACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCAAAATG CATTCGAACA TTAACATCTT CAGGTCAAGT TACCCTTGGA 
GAAGCGTGTT CTGCCAGCAC CAGCCGGACA GTAGCTATTC CTTCCGGGGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACT TTCCTCTACG CGGCCTCTGG AAATGCCGTC 
AGAATGTGGG ACCTTAAAAG GTTTCAGTCT ACAGGAAAGC TAACCGGACA CCTGGGTCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AATGGACAGG ACCTCATCAT CACTGGCTCA 
AAAGACCACT ACATCAAAAT GTTCGATGTG ACTGAAGGGG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TCGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGCGAT 
AACCTATTCA GTGGGTCCAG AGATAATGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGT 
CTTCTTCAGC AAGTCCCAAA TGCACACAAA GACTGGGTGT GTGCCCTGGG CCTGGTGCCA 
GGCCATCCGG TTTTGCTGAG TGGCTGCAGA GGCGGCATTC TGAAACTCTG GAATGTGGAC 
ACTTTTGTGC CCGTTGGAGA GATGAGAGGT CATGACAGTC CCATCAATGC CATTTGTGTT 
AACTCCACCC ATGTCTTTAC TGCTGCTGAT GATCGAACCG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAACT CTCTGACACC GGCGATCTGG GGGAGGATAT TGCCAGTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017316479.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171968.1 Mus musculus kinesin family member 21A (Kif21a), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OMu79044
Clone ID Related Accession (Same CDS sequence)	XM_017316479.1 , XM_017316479.2
Accession Version	XM_017316479.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kinesin-like protein KIF21A isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATAGGCATAA TCAACCCATT TCCTGCCTGC AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC 
GTTCACATAG CTGAAGGGCA CACCAAGGCT GTGCTCTGTG TGGATTCTAC GGATGATCTT 
CTCTTCACCG GATCAAAAGA TCGCACTTGT AAAGTATGGA ATCTCGTGAC TGGACAGGAA 
ATTATGTCCC TGGGGGTTCA CCCTAACAAC GTGGTGTCTG TAAAATACTG TAATTATACC 
AGTCTGGTCT TCACCGTGTC GACATCTTAT ATTAAGGTGT GGGATATCAG AGAGTCAGCA 
AAATGCATTC GAACATTAAC ATCTTCAGGT CAAGTTACCC TTGGAGAAGC GTGTTCTGCC 
AGCACCAGCC GGACAGTAGC TATTCCTTCC GGGGAGAGCC AGATCAATCA AATTGCACTA 
AACCCAACTG GCACTTTCCT CTACGCGGCC TCTGGAAATG CCGTCAGAAT GTGGGACCTT 
AAAAGGTTTC AGTCTACAGG AAAGCTAACC GGACACCTGG GTCCTGTTAT GTGCCTTACG 
GTAGACCAGA TCTCCAATGG ACAGGACCTC ATCATCACTG GCTCAAAAGA CCACTACATC 
AAAATGTTCG ATGTGACTGA AGGGGCTCTT GGAACTGTAA GTCCCACCCA CAACTTCGAG 
CCTCCTCATT ATGATGGGAT AGAAGCACTG GCCATTCAAG GCGATAACCT ATTCAGTGGG 
TCCAGAGATA ATGGAATCAA GAAATGGGAC TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC 
CCAAATGCAC ACAAAGACTG GGTGTGTGCC CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG 
CTGAGTGGCT GCAGAGGCGG CATTCTGAAA CTCTGGAATG TGGACACTTT TGTGCCCGTT 
GGAGAGATGA GAGGTCATGA CAGTCCCATC AATGCCATTT GTGTTAACTC CACCCATGTC 
TTTACTGCTG CTGATGATCG AACCGTGAGA ATCTGGAAGG CCCACAACTT GCAAGATGGT 
CAACTCTCTG ACACCGGCGA TCTGGGGGAG GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171968.1
CDS	2461..7188
Translation	MGPATWACVHGCWRVRSYDPWSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVAGKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTETSGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRTVRIWKAHNLQDGQLSDTGDLGEDIASN

Target ORF information:

RefSeq Version	XM_017316479.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus kinesin family member 21A (Kif21a), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316479.1

ORF Insert Sequence:

ATGGGTCCTG CTACTTGGGC ATGTGTGCAT GGCTGCTGGA GAGTCAGATC TTACGACCCA 
TGGAGTAGAA TAAGACCACA GCTTGCCAAA GAGAAGATCG AAGGTTGCCA TATCTGCACG 
TCAGTCACAC CGGGAGAGCC TCAGGTCTTC CTCGGGAAGG ATAAGGCCTT TACTTTTGAT 
TATGTGTTTG ACATTGACTC CCAGCAGGAG CAGATCTACA CCCAGTGCAT CGAAAAGCTC 
ATTGAAGGCT GTTTTGAAGG CTACAATGCC ACCGTGTTTG CCTATGGACA AACCGGAGCT 
GGGAAAACCT ACACGATGGG AACCGGATTT GACGTGAACA TCATGGAGGA AGAGCAGGGC 
ATCATCTCTC GTGCTGTTAG ACACCTGTTC AAGAGTATTG ATGAGAAAAA GACCTCAGCG 
ATTAAAAACG GGCTGCCCCC TCCTGAATTC AAAGTGAATG CCCAGTTCCT AGAGCTCTAT 
AATGAAGAGG TCCTTGACTT GTTTGATACC ACTCGGGATA TTGATGCAAA AAATAAAAAA 
TCAAATATAA GAATTCATGA AGATTCAACT GGAGGAATTT ATACTGTGGG CGTCACAACA 
CGCACTGTGA ATACAGAACC GGAGATGATG CAGTGTCTGA AGCTGGGCGC TCTCTCGCGC 
ACCACGGCCA GCACCCAGAT GAACGTACAG AGCTCTCGTT CACACGCCAT CTTTACCATT 
CACGTGTGTC AAACCAGAGT GTGTCCCCAA ACAGATGCTG AGAACGCAAC TGATAATAAG 
CTGATCTCCG AATCGTCGCC AATGAATGAG TTTGAGACGC TGACGGCGAA GTTTCACTTT 
GTTGATCTGG CGGGATCGGA AAGACTGAAG AGAACCGGAG CTACAGGCGA GAGAGCAAAA 
GAGGGCATTT CCATCAACTG CGGGCTTTTG GCTCTTGGGA ATGTAATCAG CGCCTTGGGG 
GACAAGAGCA AGAGAGCCAC CCATGTCCCT TACAGAGACT CTAAGCTGAC AAGACTCTTG 
CAGGATTCCC TTGGGGGTAA CAGCCAAACC ATCATGATAG CATGCGTCAG CCCTTCAGAC 
AGGGATTTCA TGGAGACGTT AAACACTCTG AAGTACGCCA ATCGAGCGAG AAATATCAAG 
AACAAGGTGA TGGTCAATCA AGACAGAGCC AGTCAGCAAA TCAACGCGCT GCGGAGCGAG 
ATCACGCGCC TTCAGATGGA GCTCATGGAA TACAAAACCG GTAAAAGAAT AATTGACGAG 
GAAGGCGTGG AAAGCATCAA TGACATGTTT CATGAGAATG CTATGCTGCA GACGGAAAAT 
AATAATCTGC GTGTAAGAAT AAAAGCCATG CAGGAGACCG TTGATGCACT GAGGGCCAGA 
ATCACGCAGC TTGTCAGTGA GCAGGCCAAC CAAGTTCTTG CCCGAGCAGG TGAAGGGAAC 
GAAGAGATCA GTAATATGAT TCATAGTTAT ATCAAAGAAA TTGAAGACCT CAGGGCAAAA 
TTATTAGAAA GTGAAGCAGT GAACGAGAAC CTTCGGAAGA ACTTGACCAG AGCCACGGCG 
AGATCTCCTT ACTTCAGTGC CTCCTCAGCT TTCTCGCCTA CTATACTGTC TTCAGACAAG 
GAGACTATCG AAATTATAGA TCTAGCAAAG AAAGACTTGG AGAAGCTAAA ACGGAAAGAG 
AAGAAGAAGA AGAAAAGTGT TGCCGGGAAA GACGATAATG CAGACACTGA CCAGGAGAAG 
AAAGAAGAAA AGGGTGTTTC AGAGAAAGAA AACAATGAGC TAGACGTGGA AGAGAATCAA 
GAAGTGAGTG ACCACGAGGA TGAGGAAGAG GAGGAAGAGG ACGAGGAGGA AGAGGATGAC 
ATTGAAGGAG AAGAAAGCTC TGATGAATCA GATTCCGAAT CTGATGAAAA AGCTAACTAT 
CAAGCCGACT TAGCAAATAT CACCTGCGAG ATTGCGATTA AGCAGAAGCT GATCGACGAA 
CTGGAGAACA GCCAGAAACG GCTGCAGACC CTGAAAAAGC AGTACGAGGA GAAGCTGATG 
ATGCTCCAAC ATAAGATCCG GGACACGCAG CTGGAGAGGG ACCAGGTGCT CCAGAACCTA 
GGCTCGGTGG AGTCATACTC GGAAGAAAAG GCCAAGAAAG TGAAGTGCGA ATATGAGAAG 
AAGCTCCACG CCATGAACAA AGAGCTGCAG CGACTGCAGA CGGCCCAAAA GGAGCACGCC 
AGGCTCCTCA AAAACCAGTC TCAGTATGAA AAGCAGCTCA AGAAACTGCA GCAAGACGTC 
ATGGAAATGA AGAAAACCAA GGTTCGTCTA ATGAAGCAGA TGAAAGAAGA GCAGGAGAAA 
GCCCGGCTGA CAGAATCTCG AAGGAACCGG GAAATCGCTC AGCTGAAGAA GGATCAGCGC 
AAGCGAGATC ATCAACTTAG ACTTCTAGAG GCCCAGAAAA GAAATCAAGA AGTAGTTCTG 
CGACGCAAAA CGGAAGAGGT TACAGCTCTC CGCAGGCAAG TGAGGCCCAT GTCTGATAAA 
GTAGCGGGAA AAGTTACTCG GAAGCTGAGC TCATCCGAAA GCCCCGCTCC GGACACAGGT 
TCCAGTGCGG CTTCCGGGGA AGCAGACACA TCACGGCCAG GCACCCAGCA GAAAATGAGG 
ATCCCCGTGG CAAGAGTCCA GGCATTACCA ACACCTACAA CAAATGGCAC CAGGAAAAAA 
TATCAGAGGA AAGGATTCAC TGGCCGGGTG TTCACTTCCA AGACAGCCCG CATGAAGTGG 
CAGCTACTGG AGCGCCGGGT GACCGACATC ATCATGCAGA AAATGACCAT CTCCAACATG 
GAGGCGGACA TGAACAGACT CCTCAGGCAA CGGGAAGAAC TCACAAAAAG GCGAGAGAAA 
CTTTCTAAAA GGAGAGAGAA GATAGTCAAG GAGAGCGGAG AGGGAGATAA AAGTGTGGCT 
AACATCATCG AGGAGATGGA GTCCCTGACA GCCAACATAG ATTACATCAA TGATAGCATT 
GCCGACTGTC AAGCCAACAT CATGCAGATG GAGGAGGCAA AGGAAGAAGG GGAGACATTG 
GATGTCACCG CTGTCATTAA TGCCTGTACA CTGACAGAAG CTCGGTACCT GCTAGATCAC 
TTCCTGTCAA TGGGCATCAA TAAGGGTCTG CAGGCTGCCC AGAAAGAGGC TCAAATTAAA 
GTCCTCGAGG GTCGACTCAA ACAGACCGAA ATCACCAGTG CAACCCAGAA CCAACTCTTA 
TTCCATATGC TGAAGGAGAA GGCAGAGCTA AACCCAGAGC TGGATGCCTT GCTAGGCCAC 
GCACTGCAAG ATCTAGATGG CGCCCCACCA GAAAACGAGG AGGACAGCAG CGAGGAAGAT 
GGCCCTTTAC ACAGCCCGGG GTCGGAGGGA AGCACGTTAT CTTCAGACCT TATGAAGCTT 
TGTGGTGAAG TGAAACCAAA GAACAAGGCT CGAAGGAGAA CCACCACTCA GATGGAGTTG 
CTGTATGCAG ATAGCAGTGA AGTAGCCTCA GACACTAGTG CAGGAGATGC CTCCTTGTCT 
GGCCCTCTGG CACCTGTTGC AGAAGGGCAG GAGATTGGAA TGAACACAGA GACAAGTGGT 
ACTTCTGCTA GGGACAAAGA GCTTCTTGCC CCATCTGGCT TACCTTCTAA GATAGGCAGC 
ATAGGCATAA TCAACCCATT TCCTGCCTGC AAAGGAGTCA GAGCCTCTCC TCTTCAGTGC 
GTTCACATAG CTGAAGGGCA CACCAAGGCT GTGCTCTGTG TGGATTCTAC GGATGATCTT 
CTCTTCACCG GATCAAAAGA TCGCACTTGT AAAGTATGGA ATCTCGTGAC TGGACAGGAA 
ATTATGTCCC TGGGGGTTCA CCCTAACAAC GTGGTGTCTG TAAAATACTG TAATTATACC 
AGTCTGGTCT TCACCGTGTC GACATCTTAT ATTAAGGTGT GGGATATCAG AGAGTCAGCA 
AAATGCATTC GAACATTAAC ATCTTCAGGT CAAGTTACCC TTGGAGAAGC GTGTTCTGCC 
AGCACCAGCC GGACAGTAGC TATTCCTTCC GGGGAGAGCC AGATCAATCA AATTGCACTA 
AACCCAACTG GCACTTTCCT CTACGCGGCC TCTGGAAATG CCGTCAGAAT GTGGGACCTT 
AAAAGGTTTC AGTCTACAGG AAAGCTAACC GGACACCTGG GTCCTGTTAT GTGCCTTACG 
GTAGACCAGA TCTCCAATGG ACAGGACCTC ATCATCACTG GCTCAAAAGA CCACTACATC 
AAAATGTTCG ATGTGACTGA AGGGGCTCTT GGAACTGTAA GTCCCACCCA CAACTTCGAG 
CCTCCTCATT ATGATGGGAT AGAAGCACTG GCCATTCAAG GCGATAACCT ATTCAGTGGG 
TCCAGAGATA ATGGAATCAA GAAATGGGAC TTAGCTCAGA AAGGTCTTCT TCAGCAAGTC 
CCAAATGCAC ACAAAGACTG GGTGTGTGCC CTGGGCCTGG TGCCAGGCCA TCCGGTTTTG 
CTGAGTGGCT GCAGAGGCGG CATTCTGAAA CTCTGGAATG TGGACACTTT TGTGCCCGTT 
GGAGAGATGA GAGGTCATGA CAGTCCCATC AATGCCATTT GTGTTAACTC CACCCATGTC 
TTTACTGCTG CTGATGATCG AACCGTGAGA ATCTGGAAGG CCCACAACTT GCAAGATGGT 
CAACTCTCTG ACACCGGCGA TCTGGGGGAG GATATTGCCA GTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural analyses of key features in the KANK1?KIF21A complex yield mechanistic insights into the cross-talk between microtubules and the cell cortex.
The Journal of biological chemistry293(1)215-225(2018 Jan)
Weng Z,Shang Y,Yao D,Zhu J,Zhang R

Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex.
Cell166(5)1147-1162.e15(2016 Aug)
Zhang X,Chen MH,Wu X,Kodani A,Fan J,Doan R,Ozawa M,Ma J,Yoshida N,Reiter JF,Black DL,Kharchenko PV,Sharp PA,Walsh CA

Human CFEOM1 mutations attenuate KIF21A autoinhibition and cause oculomotor axon stalling.
Neuron82(2)334-49(2014 Apr)
Cheng L,Desai J,Miranda CJ,Duncan JS,Qiu W,Nugent AA,Kolpak AL,Wu CC,Drokhlyansky E,Delisle MM,Chan WM,Wei Y,Propst F,Reck-Peterson SL,Fritzsch B,Engle EC

Spatiotemporal expression pattern of KIF21A during normal embryonic development and in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).
Gene expression patterns : GEP12(5-6)180-8(2012 May-Jun)
Desai J,Velo MP,Yamada K,Overman LM,Engle EC

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Kif21a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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