Kif21a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif21a
Entrez Gene ID	300158
Full Name	kinesin family member 21A
General protein information	Preferred Names kinesin family member 21A Names kinesin-like protein KIF21A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q35

mRNA and Protein(s)

mRNA	Protein	Name
XM_008776562.2	XP_008774784.1	kinesin-like protein KIF21A isoform X22
XM_017603484.1	XP_017458973.1	kinesin-like protein KIF21A isoform X23
XM_008776570.2	XP_008774792.1	kinesin-like protein KIF21A isoform X36
XM_008776571.2	XP_008774793.1	kinesin-like protein KIF21A isoform X37
XM_008776572.2	XP_008774794.1	kinesin-like protein KIF21A isoform X38
XM_017595289.1	XP_017450778.1	kinesin-like protein KIF21A isoform X1
XM_017595292.1	XP_017450781.1	kinesin-like protein KIF21A isoform X5
XM_017595297.1	XP_017450786.1	kinesin-like protein KIF21A isoform X12
XM_017595290.1	XP_017450779.1	kinesin-like protein KIF21A isoform X3
XM_017595295.1	XP_017450784.1	kinesin-like protein KIF21A isoform X8
XM_008765830.2	XP_008764052.1	kinesin-like protein KIF21A isoform X10
XM_008776563.2	XP_008774785.1	kinesin-like protein KIF21A isoform X25
XM_017603487.1	XP_017458976.1	kinesin-like protein KIF21A isoform X27
XM_008776564.2	XP_008774786.1	kinesin-like protein KIF21A isoform X28
XM_008776566.2	XP_008774788.1	kinesin-like protein KIF21A isoform X30
XM_008776561.2	XP_008774783.1	kinesin-like protein KIF21A isoform X21
XM_017595296.1	XP_017450785.1	kinesin-like protein KIF21A isoform X11
XM_017595299.1	XP_017450788.1	kinesin-like protein KIF21A isoform X16
XM_008765833.2	XP_008764055.1	kinesin-like protein KIF21A isoform X17
XM_017595294.1	XP_017450783.1	kinesin-like protein KIF21A isoform X7
XM_008765834.2	XP_008764056.1	kinesin-like protein KIF21A isoform X18
XM_017603483.1	XP_017458972.1	kinesin-like protein KIF21A isoform X20
XM_008776567.2	XP_008774789.1	kinesin-like protein KIF21A isoform X32
XM_008776568.2	XP_008774790.1	kinesin-like protein KIF21A isoform X33
XM_008776569.2	XP_008774791.1	kinesin-like protein KIF21A isoform X34
NM_001371538.1	NP_001358467.1	kinesin-like protein KIF21A
XM_017603485.1	XP_017458974.1	kinesin-like protein KIF21A isoform X24
XM_017603488.1	XP_017458977.1	kinesin-like protein KIF21A isoform X31
XM_017603489.1	XP_017458978.1	kinesin-like protein KIF21A isoform X35
XM_017595291.1	XP_017450780.1	kinesin-like protein KIF21A isoform X4
XM_017595293.1	XP_017450782.1	kinesin-like protein KIF21A isoform X6
XM_008765829.2	XP_008764051.1	kinesin-like protein KIF21A isoform X9
XM_008765832.2	XP_008764054.1	kinesin-like protein KIF21A isoform X14
XM_008765835.2	XP_008764057.1	kinesin-like protein KIF21A isoform X19
XM_008765828.2	XP_008764050.1	kinesin-like protein KIF21A isoform X2
XM_017603486.1	XP_017458975.1	kinesin-like protein KIF21A isoform X26
XM_008776565.2	XP_008774787.1	kinesin-like protein KIF21A isoform X29
XM_008765831.2	XP_008764053.1	kinesin-like protein KIF21A isoform X13
XM_017595298.1	XP_017450787.1	kinesin-like protein KIF21A isoform X15

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	kif21a	XP_001920258.4
Canis lupus familiaris (dog)	KIF21A	XP_003433563.1
Mus musculus (house mouse)	Kif21a	NP_001102511.1
Gallus gallus (chicken)	KIF21A	XP_004934816.1
Drosophila melanogaster (fruit fly)	Klp31E	NP_001162937.2
Rattus norvegicus (Norway rat)	Kif21a	XP_006242318.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007815	XP_317685.4
Caenorhabditis elegans (roundworm)	klp-12	NP_001023349.1
Homo sapiens (human)	KIF21A	NP_001166935.1
	KIF21A	XP_002798575.1
Bos taurus (cattle)	KIF21A	NP_001193847.1
Xenopus tropicalis (tropical clawed frog)	kif21a	NP_001182593.1
Pan troglodytes (chimpanzee)	KIF21A	XP_003313814.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).
Yamada K, Andrews C, Chan WM, McKeown CA, Magli A, de Berardinis T, Loewenstein A, Lazar M, O'Keefe M, Letson R, London A, Ruttum M, Matsumoto N, Saito N, Morris L, Del Monte M, Johnson RH, Uyama E, Houtman WA, de Vries B, Carlow TJ, Hart BL, Krawiecki N, Shoffner J, Vogel MC, Katowitz J, Goldstein SM, Levin AV, Sener EC, Ozturk BT, Akarsu AN, Brodsky MC, Hanisch F, Cruse RP, Zubcov AA, Robb RM, Roggenk?emper P, Gottlob I, Kowal L, Battu R, Traboulsi EI, Franceschini P, Newlin A, Demer JL, Engle EC
Nature genetics35(4)318-21(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Kif21a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif21a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa45679	NM_001371538.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa55794	XM_017595291.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55794	XM_017603484.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55795	XM_017595292.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55795	XM_017603485.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55796	XM_017595294.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55796	XM_017603486.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55797	XM_017595295.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55797	XM_017603487.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55798	XM_017595297.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55798	XM_017603488.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55799	XM_017595299.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa55799	XM_017603489.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ORa45680	XM_017595290.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45680	XM_008776562.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45681	XM_008776563.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45681	XM_017595293.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45682	XM_008776564.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45682	XM_008765829.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45683	XM_008765830.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45683	XM_008776565.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45684	XM_017595296.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45684	XM_008776566.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45685	XM_008765831.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45685	XM_008776567.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45686	XM_008765832.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45686	XM_008776568.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45687	XM_008776569.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45687	XM_017595298.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45688	XM_008765833.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45688	XM_008776570.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45689	XM_008765834.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45689	XM_008776571.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45690	XM_008776572.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45690	XM_008765835.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa45679	XM_008776561.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa45679	XM_008765828.2 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa55793	XM_017603483.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa55793	XM_017595289.1 Latest version!	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif21a ( NM_001371538.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001358467.1 Rattus norvegicus kinesin family member 21A (Kif21a), mRNA.

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CloneID	ORa45679
Clone ID Related Accession (Same CDS sequence)	XM_008776561.2 , NM_001371538.1 , XM_008765828.2
Accession Version	NM_001371538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-16
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07058693.1, AABR07058692.1, AABR07058690.1, AABR07058689.1 and FQ096185.1. On or before Jul 17, 2019 this sequence version replaced XM_008765828.2, XM_008776561.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGGGGCATT 
ATCAACCCAT TTCCTGCCTG CAGAGGAATC AGAGCGTCTT TCCTTCAGTG TGTTCACGTA 
GCTGAGGGGC ACACGAAAGC TGTGCTCTGC GTGGATTCGA CTGATGACCT TCTCTTCACT 
GGATCAAAAG ATCGTACTTG TAAAGTTTGG AATCTCGTGA CTGGACAGGA AATTATGTCA 
TTAGGGGTTC ACCCTAACAA CGTTGTGTCT GTAAAATACT GTAATTACAC CAGTCTAGTC 
TTCACAGTGT CAACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC GAAGTGCATT 
CGAACATTAA CGTCTTCAGG CCAAGTTACT CTGGGAGAAG CTTGTTCTGC AAGCACCAGC 
CGGACAGTGG CTATTCCTTC CGGAGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACATTCC TCTACGCGGC CTCTGGAAAT GCTGTCAGAA TGTGGGACCT CAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC GGGACACCTG GGCCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAACG GACAGGACCT AATCATCACG GGCTCAAAAG ACCACTACAT CAAAATGTTT 
GATGTGACTG AAGGAGCTCT TGGAACTGTA AGTCCCACCC ACAACTTTGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGTGATAACT TATTCAGTGG GTCCAGAGAC 
AACGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGCCTTC TTCAGCAAGT CCCAAATGCA 
CATAAGGACT GGGTCTGTGC CCTGGGCTTG GTGCCAGGCC ACCCGATTTT GCTTAGTGGC 
TGCAGAGGGG GCATTCTGAA ACTCTGGAAT GTGGATACTT TCGTGCCAGT CGGAGAGATG 
AGAGGCCATG ACAGCCCCAT CAATGCCATA AGTGTTAACT CCACCCATGT CTTCACTGCA 
GCCGATGATC GAACAGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAAATCTCT 
GACACAGGCG ATCCGGGGGA GGACAGTACC AGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358467.1
CDS	154..5172
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESSREERSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	NM_001371538.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371538.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGGGGCATT 
ATCAACCCAT TTCCTGCCTG CAGAGGAATC AGAGCGTCTT TCCTTCAGTG TGTTCACGTA 
GCTGAGGGGC ACACGAAAGC TGTGCTCTGC GTGGATTCGA CTGATGACCT TCTCTTCACT 
GGATCAAAAG ATCGTACTTG TAAAGTTTGG AATCTCGTGA CTGGACAGGA AATTATGTCA 
TTAGGGGTTC ACCCTAACAA CGTTGTGTCT GTAAAATACT GTAATTACAC CAGTCTAGTC 
TTCACAGTGT CAACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC GAAGTGCATT 
CGAACATTAA CGTCTTCAGG CCAAGTTACT CTGGGAGAAG CTTGTTCTGC AAGCACCAGC 
CGGACAGTGG CTATTCCTTC CGGAGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACATTCC TCTACGCGGC CTCTGGAAAT GCTGTCAGAA TGTGGGACCT CAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC GGGACACCTG GGCCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAACG GACAGGACCT AATCATCACG GGCTCAAAAG ACCACTACAT CAAAATGTTT 
GATGTGACTG AAGGAGCTCT TGGAACTGTA AGTCCCACCC ACAACTTTGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGTGATAACT TATTCAGTGG GTCCAGAGAC 
AACGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGCCTTC TTCAGCAAGT CCCAAATGCA 
CATAAGGACT GGGTCTGTGC CCTGGGCTTG GTGCCAGGCC ACCCGATTTT GCTTAGTGGC 
TGCAGAGGGG GCATTCTGAA ACTCTGGAAT GTGGATACTT TCGTGCCAGT CGGAGAGATG 
AGAGGCCATG ACAGCCCCAT CAATGCCATA AGTGTTAACT CCACCCATGT CTTCACTGCA 
GCCGATGATC GAACAGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAAATCTCT 
GACACAGGCG ATCCGGGGGA GGACAGTACC AGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603484.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458973.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.

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CloneID	ORa55794
Clone ID Related Accession (Same CDS sequence)	XM_017603484.1 , XM_017595291.1
Accession Version	XM_017603484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458973.1
CDS	190..5181
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603484.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603484.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595291.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450780.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

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CloneID	ORa55794
Clone ID Related Accession (Same CDS sequence)	XM_017603484.1 , XM_017595291.1
Accession Version	XM_017595291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450780.1
CDS	164..5155
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595291.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595291.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595292.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450781.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

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CloneID	ORa55795
Clone ID Related Accession (Same CDS sequence)	XM_017595292.1 , XM_017603485.1
Accession Version	XM_017595292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGATG AAGAGGACAG CAGTGAGGAA GATGGCCCTT TACACAGCCC GGGGTCAGAG 
GGAAGCACGT TGTCTTCGGA CCTGATGAAG CTTTGTGGCG AGGTGAAACC TAAGAACAAG 
GCTCGAAGGA GAACCACCAC TCAAATGGAG TTGCTGTATG CAGATAGCAG TGAAGTAGCC 
TCAGACACTA GTGCAGGAGA CGCCTCCTTG CCTGGCCCTC TGACGCCTGT TGCAGAAGGG 
CAAGAGACTG GAATGAATAC AGAGACAAGT GTCCCTTCTG CTAGGGACAA AGAGCTTCTC 
CCCCCATCTG GCTTACCTCC TAAGATAGGC AGCATTTCCA GGCAGCCATC TGTATCAGAA 
AAAAAAGTAC CAGAGCCTTC CCCTGTAACA AGGAGAAAGG CATATGAGAA AGCAGACAAA 
GCAAAGACCA AGGAGCAGAA ACACTCAGAT TCTGGCGCTT CCGAGACTAG CCTCTCACCT 
CCTTCCTCCC CACCAAGCCG GCCCCGTAAC GAACTGAATG TGTTTAATCG CCTTACCGTT 
TCTCAGGGAA GTGCGCCAGT TCAGCAGGAT AAGTCTGATG AAAGTGACCC CTCTTTGTCG 
GAGGTACACA GCAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450781.1
CDS	164..5155
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595292.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595292.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGATG AAGAGGACAG CAGTGAGGAA GATGGCCCTT TACACAGCCC GGGGTCAGAG 
GGAAGCACGT TGTCTTCGGA CCTGATGAAG CTTTGTGGCG AGGTGAAACC TAAGAACAAG 
GCTCGAAGGA GAACCACCAC TCAAATGGAG TTGCTGTATG CAGATAGCAG TGAAGTAGCC 
TCAGACACTA GTGCAGGAGA CGCCTCCTTG CCTGGCCCTC TGACGCCTGT TGCAGAAGGG 
CAAGAGACTG GAATGAATAC AGAGACAAGT GTCCCTTCTG CTAGGGACAA AGAGCTTCTC 
CCCCCATCTG GCTTACCTCC TAAGATAGGC AGCATTTCCA GGCAGCCATC TGTATCAGAA 
AAAAAAGTAC CAGAGCCTTC CCCTGTAACA AGGAGAAAGG CATATGAGAA AGCAGACAAA 
GCAAAGACCA AGGAGCAGAA ACACTCAGAT TCTGGCGCTT CCGAGACTAG CCTCTCACCT 
CCTTCCTCCC CACCAAGCCG GCCCCGTAAC GAACTGAATG TGTTTAATCG CCTTACCGTT 
TCTCAGGGAA GTGCGCCAGT TCAGCAGGAT AAGTCTGATG AAAGTGACCC CTCTTTGTCG 
GAGGTACACA GCAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603485.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458974.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.

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CloneID	ORa55795
Clone ID Related Accession (Same CDS sequence)	XM_017595292.1 , XM_017603485.1
Accession Version	XM_017603485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGATG AAGAGGACAG CAGTGAGGAA GATGGCCCTT TACACAGCCC GGGGTCAGAG 
GGAAGCACGT TGTCTTCGGA CCTGATGAAG CTTTGTGGCG AGGTGAAACC TAAGAACAAG 
GCTCGAAGGA GAACCACCAC TCAAATGGAG TTGCTGTATG CAGATAGCAG TGAAGTAGCC 
TCAGACACTA GTGCAGGAGA CGCCTCCTTG CCTGGCCCTC TGACGCCTGT TGCAGAAGGG 
CAAGAGACTG GAATGAATAC AGAGACAAGT GTCCCTTCTG CTAGGGACAA AGAGCTTCTC 
CCCCCATCTG GCTTACCTCC TAAGATAGGC AGCATTTCCA GGCAGCCATC TGTATCAGAA 
AAAAAAGTAC CAGAGCCTTC CCCTGTAACA AGGAGAAAGG CATATGAGAA AGCAGACAAA 
GCAAAGACCA AGGAGCAGAA ACACTCAGAT TCTGGCGCTT CCGAGACTAG CCTCTCACCT 
CCTTCCTCCC CACCAAGCCG GCCCCGTAAC GAACTGAATG TGTTTAATCG CCTTACCGTT 
TCTCAGGGAA GTGCGCCAGT TCAGCAGGAT AAGTCTGATG AAAGTGACCC CTCTTTGTCG 
GAGGTACACA GCAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458974.1
CDS	190..5181
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603485.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603485.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGATG AAGAGGACAG CAGTGAGGAA GATGGCCCTT TACACAGCCC GGGGTCAGAG 
GGAAGCACGT TGTCTTCGGA CCTGATGAAG CTTTGTGGCG AGGTGAAACC TAAGAACAAG 
GCTCGAAGGA GAACCACCAC TCAAATGGAG TTGCTGTATG CAGATAGCAG TGAAGTAGCC 
TCAGACACTA GTGCAGGAGA CGCCTCCTTG CCTGGCCCTC TGACGCCTGT TGCAGAAGGG 
CAAGAGACTG GAATGAATAC AGAGACAAGT GTCCCTTCTG CTAGGGACAA AGAGCTTCTC 
CCCCCATCTG GCTTACCTCC TAAGATAGGC AGCATTTCCA GGCAGCCATC TGTATCAGAA 
AAAAAAGTAC CAGAGCCTTC CCCTGTAACA AGGAGAAAGG CATATGAGAA AGCAGACAAA 
GCAAAGACCA AGGAGCAGAA ACACTCAGAT TCTGGCGCTT CCGAGACTAG CCTCTCACCT 
CCTTCCTCCC CACCAAGCCG GCCCCGTAAC GAACTGAATG TGTTTAATCG CCTTACCGTT 
TCTCAGGGAA GTGCGCCAGT TCAGCAGGAT AAGTCTGATG AAAGTGACCC CTCTTTGTCG 
GAGGTACACA GCAGATCCTC CAGAAGGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595294.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450783.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa55796
Clone ID Related Accession (Same CDS sequence)	XM_017595294.1 , XM_017603486.1
Accession Version	XM_017595294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450783.1
CDS	164..5134
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595294.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595294.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603486.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458975.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.

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CloneID	ORa55796
Clone ID Related Accession (Same CDS sequence)	XM_017595294.1 , XM_017603486.1
Accession Version	XM_017603486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458975.1
CDS	190..5160
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603486.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603486.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595295.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450784.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.

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CloneID	ORa55797
Clone ID Related Accession (Same CDS sequence)	XM_017595295.1 , XM_017603487.1
Accession Version	XM_017595295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATGA AGAGGACAGC 
AGTGAGGAAG ATGGCCCTTT ACACAGCCCG GGGTCAGAGG GAAGCACGTT GTCTTCGGAC 
CTGATGAAGC TTTGTGGCGA GGTGAAACCT AAGAACAAGG CTCGAAGGAG AACCACCACT 
CAAATGGAGT TGCTGTATGC AGATAGCAGT GAAGTAGCCT CAGACACTAG TGCAGGAGAC 
GCCTCCTTGC CTGGCCCTCT GACGCCTGTT GCAGAAGGGC AAGAGACTGG AATGAATACA 
GAGACAAGTG TCCCTTCTGC TAGGGACAAA GAGCTTCTCC CCCCATCTGG CTTACCTCCT 
AAGATAGGCA GCATTTCCAG GCAGCCATCT GTATCAGAAA AAAAAGTACC AGAGCCTTCC 
CCTGTAACAA GGAGAAAGGC ATATGAGAAA GCAGACAAAG CAAAGACCAA GGAGCAGAAA 
CACTCAGATT CTGGCGCTTC CGAGACTAGC CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACCGTTT CTCAGGGAAG TGCGCCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACCCC TCTTTGTCGG AGGTACACAG CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450784.1
CDS	164..5134
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595295.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595295.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATGA AGAGGACAGC 
AGTGAGGAAG ATGGCCCTTT ACACAGCCCG GGGTCAGAGG GAAGCACGTT GTCTTCGGAC 
CTGATGAAGC TTTGTGGCGA GGTGAAACCT AAGAACAAGG CTCGAAGGAG AACCACCACT 
CAAATGGAGT TGCTGTATGC AGATAGCAGT GAAGTAGCCT CAGACACTAG TGCAGGAGAC 
GCCTCCTTGC CTGGCCCTCT GACGCCTGTT GCAGAAGGGC AAGAGACTGG AATGAATACA 
GAGACAAGTG TCCCTTCTGC TAGGGACAAA GAGCTTCTCC CCCCATCTGG CTTACCTCCT 
AAGATAGGCA GCATTTCCAG GCAGCCATCT GTATCAGAAA AAAAAGTACC AGAGCCTTCC 
CCTGTAACAA GGAGAAAGGC ATATGAGAAA GCAGACAAAG CAAAGACCAA GGAGCAGAAA 
CACTCAGATT CTGGCGCTTC CGAGACTAGC CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACCGTTT CTCAGGGAAG TGCGCCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACCCC TCTTTGTCGG AGGTACACAG CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603487.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458976.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.

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CloneID	ORa55797
Clone ID Related Accession (Same CDS sequence)	XM_017595295.1 , XM_017603487.1
Accession Version	XM_017603487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATGA AGAGGACAGC 
AGTGAGGAAG ATGGCCCTTT ACACAGCCCG GGGTCAGAGG GAAGCACGTT GTCTTCGGAC 
CTGATGAAGC TTTGTGGCGA GGTGAAACCT AAGAACAAGG CTCGAAGGAG AACCACCACT 
CAAATGGAGT TGCTGTATGC AGATAGCAGT GAAGTAGCCT CAGACACTAG TGCAGGAGAC 
GCCTCCTTGC CTGGCCCTCT GACGCCTGTT GCAGAAGGGC AAGAGACTGG AATGAATACA 
GAGACAAGTG TCCCTTCTGC TAGGGACAAA GAGCTTCTCC CCCCATCTGG CTTACCTCCT 
AAGATAGGCA GCATTTCCAG GCAGCCATCT GTATCAGAAA AAAAAGTACC AGAGCCTTCC 
CCTGTAACAA GGAGAAAGGC ATATGAGAAA GCAGACAAAG CAAAGACCAA GGAGCAGAAA 
CACTCAGATT CTGGCGCTTC CGAGACTAGC CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACCGTTT CTCAGGGAAG TGCGCCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACCCC TCTTTGTCGG AGGTACACAG CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458976.1
CDS	190..5160
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603487.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603487.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATGA AGAGGACAGC 
AGTGAGGAAG ATGGCCCTTT ACACAGCCCG GGGTCAGAGG GAAGCACGTT GTCTTCGGAC 
CTGATGAAGC TTTGTGGCGA GGTGAAACCT AAGAACAAGG CTCGAAGGAG AACCACCACT 
CAAATGGAGT TGCTGTATGC AGATAGCAGT GAAGTAGCCT CAGACACTAG TGCAGGAGAC 
GCCTCCTTGC CTGGCCCTCT GACGCCTGTT GCAGAAGGGC AAGAGACTGG AATGAATACA 
GAGACAAGTG TCCCTTCTGC TAGGGACAAA GAGCTTCTCC CCCCATCTGG CTTACCTCCT 
AAGATAGGCA GCATTTCCAG GCAGCCATCT GTATCAGAAA AAAAAGTACC AGAGCCTTCC 
CCTGTAACAA GGAGAAAGGC ATATGAGAAA GCAGACAAAG CAAAGACCAA GGAGCAGAAA 
CACTCAGATT CTGGCGCTTC CGAGACTAGC CTCTCACCTC CTTCCTCCCC ACCAAGCCGG 
CCCCGTAACG AACTGAATGT GTTTAATCGC CTTACCGTTT CTCAGGGAAG TGCGCCAGTT 
CAGCAGGATA AGTCTGATGA AAGTGACCCC TCTTTGTCGG AGGTACACAG CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595297.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450786.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.

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CloneID	ORa55798
Clone ID Related Accession (Same CDS sequence)	XM_017595297.1 , XM_017603488.1
Accession Version	XM_017595297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGATCCT CCAGAAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450786.1
CDS	164..5026
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595297.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595297.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGATCCT CCAGAAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603488.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458977.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.

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CloneID	ORa55798
Clone ID Related Accession (Same CDS sequence)	XM_017595297.1 , XM_017603488.1
Accession Version	XM_017603488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGATCCT CCAGAAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458977.1
CDS	190..5052
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603488.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603488.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGATCCT CCAGAAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595299.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450788.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ORa55799
Clone ID Related Accession (Same CDS sequence)	XM_017595299.1 , XM_017603489.1
Accession Version	XM_017595299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450788.1
CDS	164..4918
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595299.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595299.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603489.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458978.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X35, mRNA.

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CloneID	ORa55799
Clone ID Related Accession (Same CDS sequence)	XM_017595299.1 , XM_017603489.1
Accession Version	XM_017603489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458978.1
CDS	190..4944
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603489.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603489.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776562.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774784.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.

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CloneID	ORa45680
Clone ID Related Accession (Same CDS sequence)	XM_008776562.2 , XM_017595290.1
Accession Version	XM_008776562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGCAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774784.1
CDS	190..5184
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776562.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776562.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGCAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595290.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450779.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

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CloneID	ORa45680
Clone ID Related Accession (Same CDS sequence)	XM_008776562.2 , XM_017595290.1
Accession Version	XM_017595290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGCAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450779.1
CDS	164..5158
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595290.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595290.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGTCTG ATGAAAGTGA CCCCTCTTTG 
TCGGAGGTAC ACAGCAGATC CTCCAGAAGG GGCATTATCA ACCCATTTCC TGCCTGCAGA 
GGAATCAGAG CGTCTTTCCT TCAGTGTGTT CACGTAGCTG AGGGGCACAC GAAAGCTGTG 
CTCTGCGTGG ATTCGACTGA TGACCTTCTC TTCACTGGAT CAAAAGATCG TACTTGTAAA 
GTTTGGAATC TCGTGACTGG ACAGGAAATT ATGTCATTAG GGGTTCACCC TAACAACGTT 
GTGTCTGTAA AATACTGTAA TTACACCAGT CTAGTCTTCA CAGTGTCAAC ATCTTATATT 
AAGGTGTGGG ATATCAGAGA GTCAGCGAAG TGCATTCGAA CATTAACGTC TTCAGGCCAA 
GTTACTCTGG GAGAAGCTTG TTCTGCAAGC ACCAGCCGGA CAGTGGCTAT TCCTTCCGGA 
GAGAGCCAGA TCAATCAAAT TGCACTAAAC CCAACTGGCA CATTCCTCTA CGCGGCCTCT 
GGAAATGCTG TCAGAATGTG GGACCTCAAA AGGTTTCAGT CTACAGGAAA GCTAACGGGA 
CACCTGGGCC CTGTTATGTG CCTTACGGTA GACCAGATCT CCAACGGACA GGACCTAATC 
ATCACGGGCT CAAAAGACCA CTACATCAAA ATGTTTGATG TGACTGAAGG AGCTCTTGGA 
ACTGTAAGTC CCACCCACAA CTTTGAGCCT CCTCATTATG ATGGGATAGA AGCACTGGCC 
ATTCAAGGTG ATAACTTATT CAGTGGGTCC AGAGACAACG GAATCAAGAA ATGGGACTTA 
GCTCAGAAAG GCCTTCTTCA GCAAGTCCCA AATGCACATA AGGACTGGGT CTGTGCCCTG 
GGCTTGGTGC CAGGCCACCC GATTTTGCTT AGTGGCTGCA GAGGGGGCAT TCTGAAACTC 
TGGAATGTGG ATACTTTCGT GCCAGTCGGA GAGATGAGAG GCCATGACAG CCCCATCAAT 
GCCATAAGTG TTAACTCCAC CCATGTCTTC ACTGCAGCCG ATGATCGAAC AGTGAGAATC 
TGGAAGGCCC ACAACTTGCA AGATGGTCAA ATCTCTGACA CAGGCGATCC GGGGGAGGAC 
AGTACCAGTA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776563.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774785.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.

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CloneID	ORa45681
Clone ID Related Accession (Same CDS sequence)	XM_008776563.2 , XM_017595293.1
Accession Version	XM_008776563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGCAGATCC 
TCCAGAAGGG GCATTATCAA CCCATTTCCT GCCTGCAGAG GAATCAGAGC GTCTTTCCTT 
CAGTGTGTTC ACGTAGCTGA GGGGCACACG AAAGCTGTGC TCTGCGTGGA TTCGACTGAT 
GACCTTCTCT TCACTGGATC AAAAGATCGT ACTTGTAAAG TTTGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCATTAGG GGTTCACCCT AACAACGTTG TGTCTGTAAA ATACTGTAAT 
TACACCAGTC TAGTCTTCAC AGTGTCAACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCGAAGT GCATTCGAAC ATTAACGTCT TCAGGCCAAG TTACTCTGGG AGAAGCTTGT 
TCTGCAAGCA CCAGCCGGAC AGTGGCTATT CCTTCCGGAG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC ATTCCTCTAC GCGGCCTCTG GAAATGCTGT CAGAATGTGG 
GACCTCAAAA GGTTTCAGTC TACAGGAAAG CTAACGGGAC ACCTGGGCCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAACGGACAG GACCTAATCA TCACGGGCTC AAAAGACCAC 
TACATCAAAA TGTTTGATGT GACTGAAGGA GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTTGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGTGA TAACTTATTC 
AGTGGGTCCA GAGACAACGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG CCTTCTTCAG 
CAAGTCCCAA ATGCACATAA GGACTGGGTC TGTGCCCTGG GCTTGGTGCC AGGCCACCCG 
ATTTTGCTTA GTGGCTGCAG AGGGGGCATT CTGAAACTCT GGAATGTGGA TACTTTCGTG 
CCAGTCGGAG AGATGAGAGG CCATGACAGC CCCATCAATG CCATAAGTGT TAACTCCACC 
CATGTCTTCA CTGCAGCCGA TGATCGAACA GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAA TCTCTGACAC AGGCGATCCG GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774785.1
CDS	190..5163
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776563.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776563.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGCAGATCC 
TCCAGAAGGG GCATTATCAA CCCATTTCCT GCCTGCAGAG GAATCAGAGC GTCTTTCCTT 
CAGTGTGTTC ACGTAGCTGA GGGGCACACG AAAGCTGTGC TCTGCGTGGA TTCGACTGAT 
GACCTTCTCT TCACTGGATC AAAAGATCGT ACTTGTAAAG TTTGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCATTAGG GGTTCACCCT AACAACGTTG TGTCTGTAAA ATACTGTAAT 
TACACCAGTC TAGTCTTCAC AGTGTCAACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCGAAGT GCATTCGAAC ATTAACGTCT TCAGGCCAAG TTACTCTGGG AGAAGCTTGT 
TCTGCAAGCA CCAGCCGGAC AGTGGCTATT CCTTCCGGAG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC ATTCCTCTAC GCGGCCTCTG GAAATGCTGT CAGAATGTGG 
GACCTCAAAA GGTTTCAGTC TACAGGAAAG CTAACGGGAC ACCTGGGCCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAACGGACAG GACCTAATCA TCACGGGCTC AAAAGACCAC 
TACATCAAAA TGTTTGATGT GACTGAAGGA GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTTGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGTGA TAACTTATTC 
AGTGGGTCCA GAGACAACGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG CCTTCTTCAG 
CAAGTCCCAA ATGCACATAA GGACTGGGTC TGTGCCCTGG GCTTGGTGCC AGGCCACCCG 
ATTTTGCTTA GTGGCTGCAG AGGGGGCATT CTGAAACTCT GGAATGTGGA TACTTTCGTG 
CCAGTCGGAG AGATGAGAGG CCATGACAGC CCCATCAATG CCATAAGTGT TAACTCCACC 
CATGTCTTCA CTGCAGCCGA TGATCGAACA GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAA TCTCTGACAC AGGCGATCCG GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595293.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450782.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.

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CloneID	ORa45681
Clone ID Related Accession (Same CDS sequence)	XM_008776563.2 , XM_017595293.1
Accession Version	XM_017595293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGCAGATCC 
TCCAGAAGGG GCATTATCAA CCCATTTCCT GCCTGCAGAG GAATCAGAGC GTCTTTCCTT 
CAGTGTGTTC ACGTAGCTGA GGGGCACACG AAAGCTGTGC TCTGCGTGGA TTCGACTGAT 
GACCTTCTCT TCACTGGATC AAAAGATCGT ACTTGTAAAG TTTGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCATTAGG GGTTCACCCT AACAACGTTG TGTCTGTAAA ATACTGTAAT 
TACACCAGTC TAGTCTTCAC AGTGTCAACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCGAAGT GCATTCGAAC ATTAACGTCT TCAGGCCAAG TTACTCTGGG AGAAGCTTGT 
TCTGCAAGCA CCAGCCGGAC AGTGGCTATT CCTTCCGGAG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC ATTCCTCTAC GCGGCCTCTG GAAATGCTGT CAGAATGTGG 
GACCTCAAAA GGTTTCAGTC TACAGGAAAG CTAACGGGAC ACCTGGGCCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAACGGACAG GACCTAATCA TCACGGGCTC AAAAGACCAC 
TACATCAAAA TGTTTGATGT GACTGAAGGA GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTTGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGTGA TAACTTATTC 
AGTGGGTCCA GAGACAACGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG CCTTCTTCAG 
CAAGTCCCAA ATGCACATAA GGACTGGGTC TGTGCCCTGG GCTTGGTGCC AGGCCACCCG 
ATTTTGCTTA GTGGCTGCAG AGGGGGCATT CTGAAACTCT GGAATGTGGA TACTTTCGTG 
CCAGTCGGAG AGATGAGAGG CCATGACAGC CCCATCAATG CCATAAGTGT TAACTCCACC 
CATGTCTTCA CTGCAGCCGA TGATCGAACA GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAA TCTCTGACAC AGGCGATCCG GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450782.1
CDS	164..5137
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595293.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595293.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGCAGATCC 
TCCAGAAGGG GCATTATCAA CCCATTTCCT GCCTGCAGAG GAATCAGAGC GTCTTTCCTT 
CAGTGTGTTC ACGTAGCTGA GGGGCACACG AAAGCTGTGC TCTGCGTGGA TTCGACTGAT 
GACCTTCTCT TCACTGGATC AAAAGATCGT ACTTGTAAAG TTTGGAATCT CGTGACTGGA 
CAGGAAATTA TGTCATTAGG GGTTCACCCT AACAACGTTG TGTCTGTAAA ATACTGTAAT 
TACACCAGTC TAGTCTTCAC AGTGTCAACA TCTTATATTA AGGTGTGGGA TATCAGAGAG 
TCAGCGAAGT GCATTCGAAC ATTAACGTCT TCAGGCCAAG TTACTCTGGG AGAAGCTTGT 
TCTGCAAGCA CCAGCCGGAC AGTGGCTATT CCTTCCGGAG AGAGCCAGAT CAATCAAATT 
GCACTAAACC CAACTGGCAC ATTCCTCTAC GCGGCCTCTG GAAATGCTGT CAGAATGTGG 
GACCTCAAAA GGTTTCAGTC TACAGGAAAG CTAACGGGAC ACCTGGGCCC TGTTATGTGC 
CTTACGGTAG ACCAGATCTC CAACGGACAG GACCTAATCA TCACGGGCTC AAAAGACCAC 
TACATCAAAA TGTTTGATGT GACTGAAGGA GCTCTTGGAA CTGTAAGTCC CACCCACAAC 
TTTGAGCCTC CTCATTATGA TGGGATAGAA GCACTGGCCA TTCAAGGTGA TAACTTATTC 
AGTGGGTCCA GAGACAACGG AATCAAGAAA TGGGACTTAG CTCAGAAAGG CCTTCTTCAG 
CAAGTCCCAA ATGCACATAA GGACTGGGTC TGTGCCCTGG GCTTGGTGCC AGGCCACCCG 
ATTTTGCTTA GTGGCTGCAG AGGGGGCATT CTGAAACTCT GGAATGTGGA TACTTTCGTG 
CCAGTCGGAG AGATGAGAGG CCATGACAGC CCCATCAATG CCATAAGTGT TAACTCCACC 
CATGTCTTCA CTGCAGCCGA TGATCGAACA GTGAGAATCT GGAAGGCCCA CAACTTGCAA 
GATGGTCAAA TCTCTGACAC AGGCGATCCG GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776564.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774786.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.

Price & Availability↑

CloneID	ORa45682
Clone ID Related Accession (Same CDS sequence)	XM_008776564.2 , XM_008765829.2
Accession Version	XM_008776564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGGGCA TTATCAACCC ATTTCCTGCC 
TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG TGTGTTCACG TAGCTGAGGG GCACACGAAA 
GCTGTGCTCT GCGTGGATTC GACTGATGAC CTTCTCTTCA CTGGATCAAA AGATCGTACT 
TGTAAAGTTT GGAATCTCGT GACTGGACAG GAAATTATGT CATTAGGGGT TCACCCTAAC 
AACGTTGTGT CTGTAAAATA CTGTAATTAC ACCAGTCTAG TCTTCACAGT GTCAACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCGAAGTGCA TTCGAACATT AACGTCTTCA 
GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT GCAAGCACCA GCCGGACAGT GGCTATTCCT 
TCCGGAGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACATT CCTCTACGCG 
GCCTCTGGAA ATGCTGTCAG AATGTGGGAC CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACGGGACACC TGGGCCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA CGGACAGGAC 
CTAATCATCA CGGGCTCAAA AGACCACTAC ATCAAAATGT TTGATGTGAC TGAAGGAGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTT GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGTGATAA CTTATTCAGT GGGTCCAGAG ACAACGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGCCT TCTTCAGCAA GTCCCAAATG CACATAAGGA CTGGGTCTGT 
GCCCTGGGCT TGGTGCCAGG CCACCCGATT TTGCTTAGTG GCTGCAGAGG GGGCATTCTG 
AAACTCTGGA ATGTGGATAC TTTCGTGCCA GTCGGAGAGA TGAGAGGCCA TGACAGCCCC 
ATCAATGCCA TAAGTGTTAA CTCCACCCAT GTCTTCACTG CAGCCGATGA TCGAACAGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAAATCT CTGACACAGG CGATCCGGGG 
GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774786.1
CDS	190..5130
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776564.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776564.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGGGCA TTATCAACCC ATTTCCTGCC 
TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG TGTGTTCACG TAGCTGAGGG GCACACGAAA 
GCTGTGCTCT GCGTGGATTC GACTGATGAC CTTCTCTTCA CTGGATCAAA AGATCGTACT 
TGTAAAGTTT GGAATCTCGT GACTGGACAG GAAATTATGT CATTAGGGGT TCACCCTAAC 
AACGTTGTGT CTGTAAAATA CTGTAATTAC ACCAGTCTAG TCTTCACAGT GTCAACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCGAAGTGCA TTCGAACATT AACGTCTTCA 
GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT GCAAGCACCA GCCGGACAGT GGCTATTCCT 
TCCGGAGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACATT CCTCTACGCG 
GCCTCTGGAA ATGCTGTCAG AATGTGGGAC CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACGGGACACC TGGGCCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA CGGACAGGAC 
CTAATCATCA CGGGCTCAAA AGACCACTAC ATCAAAATGT TTGATGTGAC TGAAGGAGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTT GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGTGATAA CTTATTCAGT GGGTCCAGAG ACAACGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGCCT TCTTCAGCAA GTCCCAAATG CACATAAGGA CTGGGTCTGT 
GCCCTGGGCT TGGTGCCAGG CCACCCGATT TTGCTTAGTG GCTGCAGAGG GGGCATTCTG 
AAACTCTGGA ATGTGGATAC TTTCGTGCCA GTCGGAGAGA TGAGAGGCCA TGACAGCCCC 
ATCAATGCCA TAAGTGTTAA CTCCACCCAT GTCTTCACTG CAGCCGATGA TCGAACAGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAAATCT CTGACACAGG CGATCCGGGG 
GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765829.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764051.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.

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CloneID	ORa45682
Clone ID Related Accession (Same CDS sequence)	XM_008776564.2 , XM_008765829.2
Accession Version	XM_008765829.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGGGCA TTATCAACCC ATTTCCTGCC 
TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG TGTGTTCACG TAGCTGAGGG GCACACGAAA 
GCTGTGCTCT GCGTGGATTC GACTGATGAC CTTCTCTTCA CTGGATCAAA AGATCGTACT 
TGTAAAGTTT GGAATCTCGT GACTGGACAG GAAATTATGT CATTAGGGGT TCACCCTAAC 
AACGTTGTGT CTGTAAAATA CTGTAATTAC ACCAGTCTAG TCTTCACAGT GTCAACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCGAAGTGCA TTCGAACATT AACGTCTTCA 
GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT GCAAGCACCA GCCGGACAGT GGCTATTCCT 
TCCGGAGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACATT CCTCTACGCG 
GCCTCTGGAA ATGCTGTCAG AATGTGGGAC CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACGGGACACC TGGGCCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA CGGACAGGAC 
CTAATCATCA CGGGCTCAAA AGACCACTAC ATCAAAATGT TTGATGTGAC TGAAGGAGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTT GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGTGATAA CTTATTCAGT GGGTCCAGAG ACAACGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGCCT TCTTCAGCAA GTCCCAAATG CACATAAGGA CTGGGTCTGT 
GCCCTGGGCT TGGTGCCAGG CCACCCGATT TTGCTTAGTG GCTGCAGAGG GGGCATTCTG 
AAACTCTGGA ATGTGGATAC TTTCGTGCCA GTCGGAGAGA TGAGAGGCCA TGACAGCCCC 
ATCAATGCCA TAAGTGTTAA CTCCACCCAT GTCTTCACTG CAGCCGATGA TCGAACAGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAAATCT CTGACACAGG CGATCCGGGG 
GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764051.1
CDS	164..5104
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765829.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765829.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACACTCA GATTCTGGCG CTTCCGAGAC TAGCCTCTCA 
CCTCCTTCCT CCCCACCAAG CCGGCCCCGT AACGAACTGA ATGTGTTTAA TCGCCTTACC 
GTTTCTCAGG GAAGTGCGCC AGTTCAGCAG GATAAGGGCA TTATCAACCC ATTTCCTGCC 
TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG TGTGTTCACG TAGCTGAGGG GCACACGAAA 
GCTGTGCTCT GCGTGGATTC GACTGATGAC CTTCTCTTCA CTGGATCAAA AGATCGTACT 
TGTAAAGTTT GGAATCTCGT GACTGGACAG GAAATTATGT CATTAGGGGT TCACCCTAAC 
AACGTTGTGT CTGTAAAATA CTGTAATTAC ACCAGTCTAG TCTTCACAGT GTCAACATCT 
TATATTAAGG TGTGGGATAT CAGAGAGTCA GCGAAGTGCA TTCGAACATT AACGTCTTCA 
GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT GCAAGCACCA GCCGGACAGT GGCTATTCCT 
TCCGGAGAGA GCCAGATCAA TCAAATTGCA CTAAACCCAA CTGGCACATT CCTCTACGCG 
GCCTCTGGAA ATGCTGTCAG AATGTGGGAC CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA 
ACGGGACACC TGGGCCCTGT TATGTGCCTT ACGGTAGACC AGATCTCCAA CGGACAGGAC 
CTAATCATCA CGGGCTCAAA AGACCACTAC ATCAAAATGT TTGATGTGAC TGAAGGAGCT 
CTTGGAACTG TAAGTCCCAC CCACAACTTT GAGCCTCCTC ATTATGATGG GATAGAAGCA 
CTGGCCATTC AAGGTGATAA CTTATTCAGT GGGTCCAGAG ACAACGGAAT CAAGAAATGG 
GACTTAGCTC AGAAAGGCCT TCTTCAGCAA GTCCCAAATG CACATAAGGA CTGGGTCTGT 
GCCCTGGGCT TGGTGCCAGG CCACCCGATT TTGCTTAGTG GCTGCAGAGG GGGCATTCTG 
AAACTCTGGA ATGTGGATAC TTTCGTGCCA GTCGGAGAGA TGAGAGGCCA TGACAGCCCC 
ATCAATGCCA TAAGTGTTAA CTCCACCCAT GTCTTCACTG CAGCCGATGA TCGAACAGTG 
AGAATCTGGA AGGCCCACAA CTTGCAAGAT GGTCAAATCT CTGACACAGG CGATCCGGGG 
GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765830.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764052.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.

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CloneID	ORa45683
Clone ID Related Accession (Same CDS sequence)	XM_008765830.2 , XM_008776565.2
Accession Version	XM_008765830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGGGCAT TATCAACCCA TTTCCTGCCT GCAGAGGAAT CAGAGCGTCT 
TTCCTTCAGT GTGTTCACGT AGCTGAGGGG CACACGAAAG CTGTGCTCTG CGTGGATTCG 
ACTGATGACC TTCTCTTCAC TGGATCAAAA GATCGTACTT GTAAAGTTTG GAATCTCGTG 
ACTGGACAGG AAATTATGTC ATTAGGGGTT CACCCTAACA ACGTTGTGTC TGTAAAATAC 
TGTAATTACA CCAGTCTAGT CTTCACAGTG TCAACATCTT ATATTAAGGT GTGGGATATC 
AGAGAGTCAG CGAAGTGCAT TCGAACATTA ACGTCTTCAG GCCAAGTTAC TCTGGGAGAA 
GCTTGTTCTG CAAGCACCAG CCGGACAGTG GCTATTCCTT CCGGAGAGAG CCAGATCAAT 
CAAATTGCAC TAAACCCAAC TGGCACATTC CTCTACGCGG CCTCTGGAAA TGCTGTCAGA 
ATGTGGGACC TCAAAAGGTT TCAGTCTACA GGAAAGCTAA CGGGACACCT GGGCCCTGTT 
ATGTGCCTTA CGGTAGACCA GATCTCCAAC GGACAGGACC TAATCATCAC GGGCTCAAAA 
GACCACTACA TCAAAATGTT TGATGTGACT GAAGGAGCTC TTGGAACTGT AAGTCCCACC 
CACAACTTTG AGCCTCCTCA TTATGATGGG ATAGAAGCAC TGGCCATTCA AGGTGATAAC 
TTATTCAGTG GGTCCAGAGA CAACGGAATC AAGAAATGGG ACTTAGCTCA GAAAGGCCTT 
CTTCAGCAAG TCCCAAATGC ACATAAGGAC TGGGTCTGTG CCCTGGGCTT GGTGCCAGGC 
CACCCGATTT TGCTTAGTGG CTGCAGAGGG GGCATTCTGA AACTCTGGAA TGTGGATACT 
TTCGTGCCAG TCGGAGAGAT GAGAGGCCAT GACAGCCCCA TCAATGCCAT AAGTGTTAAC 
TCCACCCATG TCTTCACTGC AGCCGATGAT CGAACAGTGA GAATCTGGAA GGCCCACAAC 
TTGCAAGATG GTCAAATCTC TGACACAGGC GATCCGGGGG AGGACAGTAC CAGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764052.1
CDS	164..5083
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765830.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765830.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGGGCAT TATCAACCCA TTTCCTGCCT GCAGAGGAAT CAGAGCGTCT 
TTCCTTCAGT GTGTTCACGT AGCTGAGGGG CACACGAAAG CTGTGCTCTG CGTGGATTCG 
ACTGATGACC TTCTCTTCAC TGGATCAAAA GATCGTACTT GTAAAGTTTG GAATCTCGTG 
ACTGGACAGG AAATTATGTC ATTAGGGGTT CACCCTAACA ACGTTGTGTC TGTAAAATAC 
TGTAATTACA CCAGTCTAGT CTTCACAGTG TCAACATCTT ATATTAAGGT GTGGGATATC 
AGAGAGTCAG CGAAGTGCAT TCGAACATTA ACGTCTTCAG GCCAAGTTAC TCTGGGAGAA 
GCTTGTTCTG CAAGCACCAG CCGGACAGTG GCTATTCCTT CCGGAGAGAG CCAGATCAAT 
CAAATTGCAC TAAACCCAAC TGGCACATTC CTCTACGCGG CCTCTGGAAA TGCTGTCAGA 
ATGTGGGACC TCAAAAGGTT TCAGTCTACA GGAAAGCTAA CGGGACACCT GGGCCCTGTT 
ATGTGCCTTA CGGTAGACCA GATCTCCAAC GGACAGGACC TAATCATCAC GGGCTCAAAA 
GACCACTACA TCAAAATGTT TGATGTGACT GAAGGAGCTC TTGGAACTGT AAGTCCCACC 
CACAACTTTG AGCCTCCTCA TTATGATGGG ATAGAAGCAC TGGCCATTCA AGGTGATAAC 
TTATTCAGTG GGTCCAGAGA CAACGGAATC AAGAAATGGG ACTTAGCTCA GAAAGGCCTT 
CTTCAGCAAG TCCCAAATGC ACATAAGGAC TGGGTCTGTG CCCTGGGCTT GGTGCCAGGC 
CACCCGATTT TGCTTAGTGG CTGCAGAGGG GGCATTCTGA AACTCTGGAA TGTGGATACT 
TTCGTGCCAG TCGGAGAGAT GAGAGGCCAT GACAGCCCCA TCAATGCCAT AAGTGTTAAC 
TCCACCCATG TCTTCACTGC AGCCGATGAT CGAACAGTGA GAATCTGGAA GGCCCACAAC 
TTGCAAGATG GTCAAATCTC TGACACAGGC GATCCGGGGG AGGACAGTAC CAGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776565.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774787.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.

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CloneID	ORa45683
Clone ID Related Accession (Same CDS sequence)	XM_008765830.2 , XM_008776565.2
Accession Version	XM_008776565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGGGCAT TATCAACCCA TTTCCTGCCT GCAGAGGAAT CAGAGCGTCT 
TTCCTTCAGT GTGTTCACGT AGCTGAGGGG CACACGAAAG CTGTGCTCTG CGTGGATTCG 
ACTGATGACC TTCTCTTCAC TGGATCAAAA GATCGTACTT GTAAAGTTTG GAATCTCGTG 
ACTGGACAGG AAATTATGTC ATTAGGGGTT CACCCTAACA ACGTTGTGTC TGTAAAATAC 
TGTAATTACA CCAGTCTAGT CTTCACAGTG TCAACATCTT ATATTAAGGT GTGGGATATC 
AGAGAGTCAG CGAAGTGCAT TCGAACATTA ACGTCTTCAG GCCAAGTTAC TCTGGGAGAA 
GCTTGTTCTG CAAGCACCAG CCGGACAGTG GCTATTCCTT CCGGAGAGAG CCAGATCAAT 
CAAATTGCAC TAAACCCAAC TGGCACATTC CTCTACGCGG CCTCTGGAAA TGCTGTCAGA 
ATGTGGGACC TCAAAAGGTT TCAGTCTACA GGAAAGCTAA CGGGACACCT GGGCCCTGTT 
ATGTGCCTTA CGGTAGACCA GATCTCCAAC GGACAGGACC TAATCATCAC GGGCTCAAAA 
GACCACTACA TCAAAATGTT TGATGTGACT GAAGGAGCTC TTGGAACTGT AAGTCCCACC 
CACAACTTTG AGCCTCCTCA TTATGATGGG ATAGAAGCAC TGGCCATTCA AGGTGATAAC 
TTATTCAGTG GGTCCAGAGA CAACGGAATC AAGAAATGGG ACTTAGCTCA GAAAGGCCTT 
CTTCAGCAAG TCCCAAATGC ACATAAGGAC TGGGTCTGTG CCCTGGGCTT GGTGCCAGGC 
CACCCGATTT TGCTTAGTGG CTGCAGAGGG GGCATTCTGA AACTCTGGAA TGTGGATACT 
TTCGTGCCAG TCGGAGAGAT GAGAGGCCAT GACAGCCCCA TCAATGCCAT AAGTGTTAAC 
TCCACCCATG TCTTCACTGC AGCCGATGAT CGAACAGTGA GAATCTGGAA GGCCCACAAC 
TTGCAAGATG GTCAAATCTC TGACACAGGC GATCCGGGGG AGGACAGTAC CAGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774787.1
CDS	190..5109
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776565.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776565.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGGGCAT TATCAACCCA TTTCCTGCCT GCAGAGGAAT CAGAGCGTCT 
TTCCTTCAGT GTGTTCACGT AGCTGAGGGG CACACGAAAG CTGTGCTCTG CGTGGATTCG 
ACTGATGACC TTCTCTTCAC TGGATCAAAA GATCGTACTT GTAAAGTTTG GAATCTCGTG 
ACTGGACAGG AAATTATGTC ATTAGGGGTT CACCCTAACA ACGTTGTGTC TGTAAAATAC 
TGTAATTACA CCAGTCTAGT CTTCACAGTG TCAACATCTT ATATTAAGGT GTGGGATATC 
AGAGAGTCAG CGAAGTGCAT TCGAACATTA ACGTCTTCAG GCCAAGTTAC TCTGGGAGAA 
GCTTGTTCTG CAAGCACCAG CCGGACAGTG GCTATTCCTT CCGGAGAGAG CCAGATCAAT 
CAAATTGCAC TAAACCCAAC TGGCACATTC CTCTACGCGG CCTCTGGAAA TGCTGTCAGA 
ATGTGGGACC TCAAAAGGTT TCAGTCTACA GGAAAGCTAA CGGGACACCT GGGCCCTGTT 
ATGTGCCTTA CGGTAGACCA GATCTCCAAC GGACAGGACC TAATCATCAC GGGCTCAAAA 
GACCACTACA TCAAAATGTT TGATGTGACT GAAGGAGCTC TTGGAACTGT AAGTCCCACC 
CACAACTTTG AGCCTCCTCA TTATGATGGG ATAGAAGCAC TGGCCATTCA AGGTGATAAC 
TTATTCAGTG GGTCCAGAGA CAACGGAATC AAGAAATGGG ACTTAGCTCA GAAAGGCCTT 
CTTCAGCAAG TCCCAAATGC ACATAAGGAC TGGGTCTGTG CCCTGGGCTT GGTGCCAGGC 
CACCCGATTT TGCTTAGTGG CTGCAGAGGG GGCATTCTGA AACTCTGGAA TGTGGATACT 
TTCGTGCCAG TCGGAGAGAT GAGAGGCCAT GACAGCCCCA TCAATGCCAT AAGTGTTAAC 
TCCACCCATG TCTTCACTGC AGCCGATGAT CGAACAGTGA GAATCTGGAA GGCCCACAAC 
TTGCAAGATG GTCAAATCTC TGACACAGGC GATCCGGGGG AGGACAGTAC CAGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776566.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774788.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.

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CloneID	ORa45684
Clone ID Related Accession (Same CDS sequence)	XM_008776566.2 , XM_017595296.1
Accession Version	XM_008776566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGCAGAT CCTCCAGAAG GGGCATTATC AACCCATTTC CTGCCTGCAG AGGAATCAGA 
GCGTCTTTCC TTCAGTGTGT TCACGTAGCT GAGGGGCACA CGAAAGCTGT GCTCTGCGTG 
GATTCGACTG ATGACCTTCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTTTGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCATTA GGGGTTCACC CTAACAACGT TGTGTCTGTA 
AAATACTGTA ATTACACCAG TCTAGTCTTC ACAGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCGAA GTGCATTCGA ACATTAACGT CTTCAGGCCA AGTTACTCTG 
GGAGAAGCTT GTTCTGCAAG CACCAGCCGG ACAGTGGCTA TTCCTTCCGG AGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACATTCCTCT ACGCGGCCTC TGGAAATGCT 
GTCAGAATGT GGGACCTCAA AAGGTTTCAG TCTACAGGAA AGCTAACGGG ACACCTGGGC 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAACGGAC AGGACCTAAT CATCACGGGC 
TCAAAAGACC ACTACATCAA AATGTTTGAT GTGACTGAAG GAGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTTGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGT 
GATAACTTAT TCAGTGGGTC CAGAGACAAC GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGCCTTCTTC AGCAAGTCCC AAATGCACAT AAGGACTGGG TCTGTGCCCT GGGCTTGGTG 
CCAGGCCACC CGATTTTGCT TAGTGGCTGC AGAGGGGGCA TTCTGAAACT CTGGAATGTG 
GATACTTTCG TGCCAGTCGG AGAGATGAGA GGCCATGACA GCCCCATCAA TGCCATAAGT 
GTTAACTCCA CCCATGTCTT CACTGCAGCC GATGATCGAA CAGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA AATCTCTGAC ACAGGCGATC CGGGGGAGGA CAGTACCAGT 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774788.1
CDS	190..5055
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776566.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776566.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGCAGAT CCTCCAGAAG GGGCATTATC AACCCATTTC CTGCCTGCAG AGGAATCAGA 
GCGTCTTTCC TTCAGTGTGT TCACGTAGCT GAGGGGCACA CGAAAGCTGT GCTCTGCGTG 
GATTCGACTG ATGACCTTCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTTTGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCATTA GGGGTTCACC CTAACAACGT TGTGTCTGTA 
AAATACTGTA ATTACACCAG TCTAGTCTTC ACAGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCGAA GTGCATTCGA ACATTAACGT CTTCAGGCCA AGTTACTCTG 
GGAGAAGCTT GTTCTGCAAG CACCAGCCGG ACAGTGGCTA TTCCTTCCGG AGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACATTCCTCT ACGCGGCCTC TGGAAATGCT 
GTCAGAATGT GGGACCTCAA AAGGTTTCAG TCTACAGGAA AGCTAACGGG ACACCTGGGC 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAACGGAC AGGACCTAAT CATCACGGGC 
TCAAAAGACC ACTACATCAA AATGTTTGAT GTGACTGAAG GAGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTTGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGT 
GATAACTTAT TCAGTGGGTC CAGAGACAAC GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGCCTTCTTC AGCAAGTCCC AAATGCACAT AAGGACTGGG TCTGTGCCCT GGGCTTGGTG 
CCAGGCCACC CGATTTTGCT TAGTGGCTGC AGAGGGGGCA TTCTGAAACT CTGGAATGTG 
GATACTTTCG TGCCAGTCGG AGAGATGAGA GGCCATGACA GCCCCATCAA TGCCATAAGT 
GTTAACTCCA CCCATGTCTT CACTGCAGCC GATGATCGAA CAGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA AATCTCTGAC ACAGGCGATC CGGGGGAGGA CAGTACCAGT 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595296.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450785.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.

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CloneID	ORa45684
Clone ID Related Accession (Same CDS sequence)	XM_008776566.2 , XM_017595296.1
Accession Version	XM_017595296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGCAGAT CCTCCAGAAG GGGCATTATC AACCCATTTC CTGCCTGCAG AGGAATCAGA 
GCGTCTTTCC TTCAGTGTGT TCACGTAGCT GAGGGGCACA CGAAAGCTGT GCTCTGCGTG 
GATTCGACTG ATGACCTTCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTTTGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCATTA GGGGTTCACC CTAACAACGT TGTGTCTGTA 
AAATACTGTA ATTACACCAG TCTAGTCTTC ACAGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCGAA GTGCATTCGA ACATTAACGT CTTCAGGCCA AGTTACTCTG 
GGAGAAGCTT GTTCTGCAAG CACCAGCCGG ACAGTGGCTA TTCCTTCCGG AGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACATTCCTCT ACGCGGCCTC TGGAAATGCT 
GTCAGAATGT GGGACCTCAA AAGGTTTCAG TCTACAGGAA AGCTAACGGG ACACCTGGGC 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAACGGAC AGGACCTAAT CATCACGGGC 
TCAAAAGACC ACTACATCAA AATGTTTGAT GTGACTGAAG GAGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTTGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGT 
GATAACTTAT TCAGTGGGTC CAGAGACAAC GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGCCTTCTTC AGCAAGTCCC AAATGCACAT AAGGACTGGG TCTGTGCCCT GGGCTTGGTG 
CCAGGCCACC CGATTTTGCT TAGTGGCTGC AGAGGGGGCA TTCTGAAACT CTGGAATGTG 
GATACTTTCG TGCCAGTCGG AGAGATGAGA GGCCATGACA GCCCCATCAA TGCCATAAGT 
GTTAACTCCA CCCATGTCTT CACTGCAGCC GATGATCGAA CAGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA AATCTCTGAC ACAGGCGATC CGGGGGAGGA CAGTACCAGT 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450785.1
CDS	164..5029
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595296.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595296.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGCAGAT CCTCCAGAAG GGGCATTATC AACCCATTTC CTGCCTGCAG AGGAATCAGA 
GCGTCTTTCC TTCAGTGTGT TCACGTAGCT GAGGGGCACA CGAAAGCTGT GCTCTGCGTG 
GATTCGACTG ATGACCTTCT CTTCACTGGA TCAAAAGATC GTACTTGTAA AGTTTGGAAT 
CTCGTGACTG GACAGGAAAT TATGTCATTA GGGGTTCACC CTAACAACGT TGTGTCTGTA 
AAATACTGTA ATTACACCAG TCTAGTCTTC ACAGTGTCAA CATCTTATAT TAAGGTGTGG 
GATATCAGAG AGTCAGCGAA GTGCATTCGA ACATTAACGT CTTCAGGCCA AGTTACTCTG 
GGAGAAGCTT GTTCTGCAAG CACCAGCCGG ACAGTGGCTA TTCCTTCCGG AGAGAGCCAG 
ATCAATCAAA TTGCACTAAA CCCAACTGGC ACATTCCTCT ACGCGGCCTC TGGAAATGCT 
GTCAGAATGT GGGACCTCAA AAGGTTTCAG TCTACAGGAA AGCTAACGGG ACACCTGGGC 
CCTGTTATGT GCCTTACGGT AGACCAGATC TCCAACGGAC AGGACCTAAT CATCACGGGC 
TCAAAAGACC ACTACATCAA AATGTTTGAT GTGACTGAAG GAGCTCTTGG AACTGTAAGT 
CCCACCCACA ACTTTGAGCC TCCTCATTAT GATGGGATAG AAGCACTGGC CATTCAAGGT 
GATAACTTAT TCAGTGGGTC CAGAGACAAC GGAATCAAGA AATGGGACTT AGCTCAGAAA 
GGCCTTCTTC AGCAAGTCCC AAATGCACAT AAGGACTGGG TCTGTGCCCT GGGCTTGGTG 
CCAGGCCACC CGATTTTGCT TAGTGGCTGC AGAGGGGGCA TTCTGAAACT CTGGAATGTG 
GATACTTTCG TGCCAGTCGG AGAGATGAGA GGCCATGACA GCCCCATCAA TGCCATAAGT 
GTTAACTCCA CCCATGTCTT CACTGCAGCC GATGATCGAA CAGTGAGAAT CTGGAAGGCC 
CACAACTTGC AAGATGGTCA AATCTCTGAC ACAGGCGATC CGGGGGAGGA CAGTACCAGT 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776567.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774789.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.

Price & Availability↑

CloneID	ORa45685
Clone ID Related Accession (Same CDS sequence)	XM_008776567.2 , XM_008765831.2
Accession Version	XM_008776567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774789.1
CDS	190..5040
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776567.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776567.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765831.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764053.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.

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CloneID	ORa45685
Clone ID Related Accession (Same CDS sequence)	XM_008776567.2 , XM_008765831.2
Accession Version	XM_008765831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764053.1
CDS	164..5014
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765831.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765831.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGTCT GATGAAAGTG ACCCCTCTTT GTCGGAGGTA 
CACAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776568.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774790.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.

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CloneID	ORa45686
Clone ID Related Accession (Same CDS sequence)	XM_008776568.2 , XM_008765832.2
Accession Version	XM_008776568.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774790.1
CDS	190..5001
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776568.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776568.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765832.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764054.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.

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CloneID	ORa45686
Clone ID Related Accession (Same CDS sequence)	XM_008776568.2 , XM_008765832.2
Accession Version	XM_008765832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764054.1
CDS	164..4975
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKQGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765832.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765832.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATTT CCAGGCAGCC ATCTGTATCA 
GAAAAAAAAG TACCAGAGCC TTCCCCTGTA ACAAGGAGAA AGGCATATGA GAAAGCAGAC 
AAAGCAAAGA CCAAGGAGCA GAAACAGGGC ATTATCAACC CATTTCCTGC CTGCAGAGGA 
ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC GTAGCTGAGG GGCACACGAA AGCTGTGCTC 
TGCGTGGATT CGACTGATGA CCTTCTCTTC ACTGGATCAA AAGATCGTAC TTGTAAAGTT 
TGGAATCTCG TGACTGGACA GGAAATTATG TCATTAGGGG TTCACCCTAA CAACGTTGTG 
TCTGTAAAAT ACTGTAATTA CACCAGTCTA GTCTTCACAG TGTCAACATC TTATATTAAG 
GTGTGGGATA TCAGAGAGTC AGCGAAGTGC ATTCGAACAT TAACGTCTTC AGGCCAAGTT 
ACTCTGGGAG AAGCTTGTTC TGCAAGCACC AGCCGGACAG TGGCTATTCC TTCCGGAGAG 
AGCCAGATCA ATCAAATTGC ACTAAACCCA ACTGGCACAT TCCTCTACGC GGCCTCTGGA 
AATGCTGTCA GAATGTGGGA CCTCAAAAGG TTTCAGTCTA CAGGAAAGCT AACGGGACAC 
CTGGGCCCTG TTATGTGCCT TACGGTAGAC CAGATCTCCA ACGGACAGGA CCTAATCATC 
ACGGGCTCAA AAGACCACTA CATCAAAATG TTTGATGTGA CTGAAGGAGC TCTTGGAACT 
GTAAGTCCCA CCCACAACTT TGAGCCTCCT CATTATGATG GGATAGAAGC ACTGGCCATT 
CAAGGTGATA ACTTATTCAG TGGGTCCAGA GACAACGGAA TCAAGAAATG GGACTTAGCT 
CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT GCACATAAGG ACTGGGTCTG TGCCCTGGGC 
TTGGTGCCAG GCCACCCGAT TTTGCTTAGT GGCTGCAGAG GGGGCATTCT GAAACTCTGG 
AATGTGGATA CTTTCGTGCC AGTCGGAGAG ATGAGAGGCC ATGACAGCCC CATCAATGCC 
ATAAGTGTTA ACTCCACCCA TGTCTTCACT GCAGCCGATG ATCGAACAGT GAGAATCTGG 
AAGGCCCACA ACTTGCAAGA TGGTCAAATC TCTGACACAG GCGATCCGGG GGAGGACAGT 
ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776569.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774791.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X34, mRNA.

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CloneID	ORa45687
Clone ID Related Accession (Same CDS sequence)	XM_008776569.2 , XM_017595298.1
Accession Version	XM_008776569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGCAG ATCCTCCAGA AGGGGCATTA TCAACCCATT TCCTGCCTGC 
AGAGGAATCA GAGCGTCTTT CCTTCAGTGT GTTCACGTAG CTGAGGGGCA CACGAAAGCT 
GTGCTCTGCG TGGATTCGAC TGATGACCTT CTCTTCACTG GATCAAAAGA TCGTACTTGT 
AAAGTTTGGA ATCTCGTGAC TGGACAGGAA ATTATGTCAT TAGGGGTTCA CCCTAACAAC 
GTTGTGTCTG TAAAATACTG TAATTACACC AGTCTAGTCT TCACAGTGTC AACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCG AAGTGCATTC GAACATTAAC GTCTTCAGGC 
CAAGTTACTC TGGGAGAAGC TTGTTCTGCA AGCACCAGCC GGACAGTGGC TATTCCTTCC 
GGAGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACATTCCT CTACGCGGCC 
TCTGGAAATG CTGTCAGAAT GTGGGACCTC AAAAGGTTTC AGTCTACAGG AAAGCTAACG 
GGACACCTGG GCCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAACGG ACAGGACCTA 
ATCATCACGG GCTCAAAAGA CCACTACATC AAAATGTTTG ATGTGACTGA AGGAGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTTGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GTGATAACTT ATTCAGTGGG TCCAGAGACA ACGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGCCTTCT TCAGCAAGTC CCAAATGCAC ATAAGGACTG GGTCTGTGCC 
CTGGGCTTGG TGCCAGGCCA CCCGATTTTG CTTAGTGGCT GCAGAGGGGG CATTCTGAAA 
CTCTGGAATG TGGATACTTT CGTGCCAGTC GGAGAGATGA GAGGCCATGA CAGCCCCATC 
AATGCCATAA GTGTTAACTC CACCCATGTC TTCACTGCAG CCGATGATCG AACAGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAAATCTCTG ACACAGGCGA TCCGGGGGAG 
GACAGTACCA GTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774791.1
CDS	190..4947
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776569.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776569.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGCAG ATCCTCCAGA AGGGGCATTA TCAACCCATT TCCTGCCTGC 
AGAGGAATCA GAGCGTCTTT CCTTCAGTGT GTTCACGTAG CTGAGGGGCA CACGAAAGCT 
GTGCTCTGCG TGGATTCGAC TGATGACCTT CTCTTCACTG GATCAAAAGA TCGTACTTGT 
AAAGTTTGGA ATCTCGTGAC TGGACAGGAA ATTATGTCAT TAGGGGTTCA CCCTAACAAC 
GTTGTGTCTG TAAAATACTG TAATTACACC AGTCTAGTCT TCACAGTGTC AACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCG AAGTGCATTC GAACATTAAC GTCTTCAGGC 
CAAGTTACTC TGGGAGAAGC TTGTTCTGCA AGCACCAGCC GGACAGTGGC TATTCCTTCC 
GGAGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACATTCCT CTACGCGGCC 
TCTGGAAATG CTGTCAGAAT GTGGGACCTC AAAAGGTTTC AGTCTACAGG AAAGCTAACG 
GGACACCTGG GCCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAACGG ACAGGACCTA 
ATCATCACGG GCTCAAAAGA CCACTACATC AAAATGTTTG ATGTGACTGA AGGAGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTTGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GTGATAACTT ATTCAGTGGG TCCAGAGACA ACGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGCCTTCT TCAGCAAGTC CCAAATGCAC ATAAGGACTG GGTCTGTGCC 
CTGGGCTTGG TGCCAGGCCA CCCGATTTTG CTTAGTGGCT GCAGAGGGGG CATTCTGAAA 
CTCTGGAATG TGGATACTTT CGTGCCAGTC GGAGAGATGA GAGGCCATGA CAGCCCCATC 
AATGCCATAA GTGTTAACTC CACCCATGTC TTCACTGCAG CCGATGATCG AACAGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAAATCTCTG ACACAGGCGA TCCGGGGGAG 
GACAGTACCA GTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595298.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450787.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ORa45687
Clone ID Related Accession (Same CDS sequence)	XM_008776569.2 , XM_017595298.1
Accession Version	XM_017595298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGCAG ATCCTCCAGA AGGGGCATTA TCAACCCATT TCCTGCCTGC 
AGAGGAATCA GAGCGTCTTT CCTTCAGTGT GTTCACGTAG CTGAGGGGCA CACGAAAGCT 
GTGCTCTGCG TGGATTCGAC TGATGACCTT CTCTTCACTG GATCAAAAGA TCGTACTTGT 
AAAGTTTGGA ATCTCGTGAC TGGACAGGAA ATTATGTCAT TAGGGGTTCA CCCTAACAAC 
GTTGTGTCTG TAAAATACTG TAATTACACC AGTCTAGTCT TCACAGTGTC AACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCG AAGTGCATTC GAACATTAAC GTCTTCAGGC 
CAAGTTACTC TGGGAGAAGC TTGTTCTGCA AGCACCAGCC GGACAGTGGC TATTCCTTCC 
GGAGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACATTCCT CTACGCGGCC 
TCTGGAAATG CTGTCAGAAT GTGGGACCTC AAAAGGTTTC AGTCTACAGG AAAGCTAACG 
GGACACCTGG GCCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAACGG ACAGGACCTA 
ATCATCACGG GCTCAAAAGA CCACTACATC AAAATGTTTG ATGTGACTGA AGGAGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTTGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GTGATAACTT ATTCAGTGGG TCCAGAGACA ACGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGCCTTCT TCAGCAAGTC CCAAATGCAC ATAAGGACTG GGTCTGTGCC 
CTGGGCTTGG TGCCAGGCCA CCCGATTTTG CTTAGTGGCT GCAGAGGGGG CATTCTGAAA 
CTCTGGAATG TGGATACTTT CGTGCCAGTC GGAGAGATGA GAGGCCATGA CAGCCCCATC 
AATGCCATAA GTGTTAACTC CACCCATGTC TTCACTGCAG CCGATGATCG AACAGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAAATCTCTG ACACAGGCGA TCCGGGGGAG 
GACAGTACCA GTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450787.1
CDS	164..4921
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHSRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595298.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595298.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGCAG ATCCTCCAGA AGGGGCATTA TCAACCCATT TCCTGCCTGC 
AGAGGAATCA GAGCGTCTTT CCTTCAGTGT GTTCACGTAG CTGAGGGGCA CACGAAAGCT 
GTGCTCTGCG TGGATTCGAC TGATGACCTT CTCTTCACTG GATCAAAAGA TCGTACTTGT 
AAAGTTTGGA ATCTCGTGAC TGGACAGGAA ATTATGTCAT TAGGGGTTCA CCCTAACAAC 
GTTGTGTCTG TAAAATACTG TAATTACACC AGTCTAGTCT TCACAGTGTC AACATCTTAT 
ATTAAGGTGT GGGATATCAG AGAGTCAGCG AAGTGCATTC GAACATTAAC GTCTTCAGGC 
CAAGTTACTC TGGGAGAAGC TTGTTCTGCA AGCACCAGCC GGACAGTGGC TATTCCTTCC 
GGAGAGAGCC AGATCAATCA AATTGCACTA AACCCAACTG GCACATTCCT CTACGCGGCC 
TCTGGAAATG CTGTCAGAAT GTGGGACCTC AAAAGGTTTC AGTCTACAGG AAAGCTAACG 
GGACACCTGG GCCCTGTTAT GTGCCTTACG GTAGACCAGA TCTCCAACGG ACAGGACCTA 
ATCATCACGG GCTCAAAAGA CCACTACATC AAAATGTTTG ATGTGACTGA AGGAGCTCTT 
GGAACTGTAA GTCCCACCCA CAACTTTGAG CCTCCTCATT ATGATGGGAT AGAAGCACTG 
GCCATTCAAG GTGATAACTT ATTCAGTGGG TCCAGAGACA ACGGAATCAA GAAATGGGAC 
TTAGCTCAGA AAGGCCTTCT TCAGCAAGTC CCAAATGCAC ATAAGGACTG GGTCTGTGCC 
CTGGGCTTGG TGCCAGGCCA CCCGATTTTG CTTAGTGGCT GCAGAGGGGG CATTCTGAAA 
CTCTGGAATG TGGATACTTT CGTGCCAGTC GGAGAGATGA GAGGCCATGA CAGCCCCATC 
AATGCCATAA GTGTTAACTC CACCCATGTC TTCACTGCAG CCGATGATCG AACAGTGAGA 
ATCTGGAAGG CCCACAACTT GCAAGATGGT CAAATCTCTG ACACAGGCGA TCCGGGGGAG 
GACAGTACCA GTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776570.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774792.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X36, mRNA.

Price & Availability↑

CloneID	ORa45688
Clone ID Related Accession (Same CDS sequence)	XM_008776570.2 , XM_008765833.2
Accession Version	XM_008776570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774792.1
CDS	190..4932
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776570.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776570.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765833.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764055.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.

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CloneID	ORa45688
Clone ID Related Accession (Same CDS sequence)	XM_008776570.2 , XM_008765833.2
Accession Version	XM_008765833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764055.1
CDS	164..4906
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765833.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765833.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGT CTGATGAAAG TGACCCCTCT 
TTGTCGGAGG TACACAGGGG CATTATCAAC CCATTTCCTG CCTGCAGAGG AATCAGAGCG 
TCTTTCCTTC AGTGTGTTCA CGTAGCTGAG GGGCACACGA AAGCTGTGCT CTGCGTGGAT 
TCGACTGATG ACCTTCTCTT CACTGGATCA AAAGATCGTA CTTGTAAAGT TTGGAATCTC 
GTGACTGGAC AGGAAATTAT GTCATTAGGG GTTCACCCTA ACAACGTTGT GTCTGTAAAA 
TACTGTAATT ACACCAGTCT AGTCTTCACA GTGTCAACAT CTTATATTAA GGTGTGGGAT 
ATCAGAGAGT CAGCGAAGTG CATTCGAACA TTAACGTCTT CAGGCCAAGT TACTCTGGGA 
GAAGCTTGTT CTGCAAGCAC CAGCCGGACA GTGGCTATTC CTTCCGGAGA GAGCCAGATC 
AATCAAATTG CACTAAACCC AACTGGCACA TTCCTCTACG CGGCCTCTGG AAATGCTGTC 
AGAATGTGGG ACCTCAAAAG GTTTCAGTCT ACAGGAAAGC TAACGGGACA CCTGGGCCCT 
GTTATGTGCC TTACGGTAGA CCAGATCTCC AACGGACAGG ACCTAATCAT CACGGGCTCA 
AAAGACCACT ACATCAAAAT GTTTGATGTG ACTGAAGGAG CTCTTGGAAC TGTAAGTCCC 
ACCCACAACT TTGAGCCTCC TCATTATGAT GGGATAGAAG CACTGGCCAT TCAAGGTGAT 
AACTTATTCA GTGGGTCCAG AGACAACGGA ATCAAGAAAT GGGACTTAGC TCAGAAAGGC 
CTTCTTCAGC AAGTCCCAAA TGCACATAAG GACTGGGTCT GTGCCCTGGG CTTGGTGCCA 
GGCCACCCGA TTTTGCTTAG TGGCTGCAGA GGGGGCATTC TGAAACTCTG GAATGTGGAT 
ACTTTCGTGC CAGTCGGAGA GATGAGAGGC CATGACAGCC CCATCAATGC CATAAGTGTT 
AACTCCACCC ATGTCTTCAC TGCAGCCGAT GATCGAACAG TGAGAATCTG GAAGGCCCAC 
AACTTGCAAG ATGGTCAAAT CTCTGACACA GGCGATCCGG GGGAGGACAG TACCAGTAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776571.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774793.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X37, mRNA.

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CloneID	ORa45689
Clone ID Related Accession (Same CDS sequence)	XM_008776571.2 , XM_008765834.2
Accession Version	XM_008776571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATGTC TGATGAAAGT GACCCCTCTT TGTCGGAGGT ACACAGGGGC 
ATTATCAACC CATTTCCTGC CTGCAGAGGA ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC 
GTAGCTGAGG GGCACACGAA AGCTGTGCTC TGCGTGGATT CGACTGATGA CCTTCTCTTC 
ACTGGATCAA AAGATCGTAC TTGTAAAGTT TGGAATCTCG TGACTGGACA GGAAATTATG 
TCATTAGGGG TTCACCCTAA CAACGTTGTG TCTGTAAAAT ACTGTAATTA CACCAGTCTA 
GTCTTCACAG TGTCAACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCGAAGTGC 
ATTCGAACAT TAACGTCTTC AGGCCAAGTT ACTCTGGGAG AAGCTTGTTC TGCAAGCACC 
AGCCGGACAG TGGCTATTCC TTCCGGAGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACAT TCCTCTACGC GGCCTCTGGA AATGCTGTCA GAATGTGGGA CCTCAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACGGGACAC CTGGGCCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ACGGACAGGA CCTAATCATC ACGGGCTCAA AAGACCACTA CATCAAAATG 
TTTGATGTGA CTGAAGGAGC TCTTGGAACT GTAAGTCCCA CCCACAACTT TGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGTGATA ACTTATTCAG TGGGTCCAGA 
GACAACGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT 
GCACATAAGG ACTGGGTCTG TGCCCTGGGC TTGGTGCCAG GCCACCCGAT TTTGCTTAGT 
GGCTGCAGAG GGGGCATTCT GAAACTCTGG AATGTGGATA CTTTCGTGCC AGTCGGAGAG 
ATGAGAGGCC ATGACAGCCC CATCAATGCC ATAAGTGTTA ACTCCACCCA TGTCTTCACT 
GCAGCCGATG ATCGAACAGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAAATC 
TCTGACACAG GCGATCCGGG GGAGGACAGT ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774793.1
CDS	190..4911
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776571.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776571.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATGTC TGATGAAAGT GACCCCTCTT TGTCGGAGGT ACACAGGGGC 
ATTATCAACC CATTTCCTGC CTGCAGAGGA ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC 
GTAGCTGAGG GGCACACGAA AGCTGTGCTC TGCGTGGATT CGACTGATGA CCTTCTCTTC 
ACTGGATCAA AAGATCGTAC TTGTAAAGTT TGGAATCTCG TGACTGGACA GGAAATTATG 
TCATTAGGGG TTCACCCTAA CAACGTTGTG TCTGTAAAAT ACTGTAATTA CACCAGTCTA 
GTCTTCACAG TGTCAACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCGAAGTGC 
ATTCGAACAT TAACGTCTTC AGGCCAAGTT ACTCTGGGAG AAGCTTGTTC TGCAAGCACC 
AGCCGGACAG TGGCTATTCC TTCCGGAGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACAT TCCTCTACGC GGCCTCTGGA AATGCTGTCA GAATGTGGGA CCTCAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACGGGACAC CTGGGCCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ACGGACAGGA CCTAATCATC ACGGGCTCAA AAGACCACTA CATCAAAATG 
TTTGATGTGA CTGAAGGAGC TCTTGGAACT GTAAGTCCCA CCCACAACTT TGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGTGATA ACTTATTCAG TGGGTCCAGA 
GACAACGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT 
GCACATAAGG ACTGGGTCTG TGCCCTGGGC TTGGTGCCAG GCCACCCGAT TTTGCTTAGT 
GGCTGCAGAG GGGGCATTCT GAAACTCTGG AATGTGGATA CTTTCGTGCC AGTCGGAGAG 
ATGAGAGGCC ATGACAGCCC CATCAATGCC ATAAGTGTTA ACTCCACCCA TGTCTTCACT 
GCAGCCGATG ATCGAACAGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAAATC 
TCTGACACAG GCGATCCGGG GGAGGACAGT ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765834.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764056.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.

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CloneID	ORa45689
Clone ID Related Accession (Same CDS sequence)	XM_008776571.2 , XM_008765834.2
Accession Version	XM_008765834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATGTC TGATGAAAGT GACCCCTCTT TGTCGGAGGT ACACAGGGGC 
ATTATCAACC CATTTCCTGC CTGCAGAGGA ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC 
GTAGCTGAGG GGCACACGAA AGCTGTGCTC TGCGTGGATT CGACTGATGA CCTTCTCTTC 
ACTGGATCAA AAGATCGTAC TTGTAAAGTT TGGAATCTCG TGACTGGACA GGAAATTATG 
TCATTAGGGG TTCACCCTAA CAACGTTGTG TCTGTAAAAT ACTGTAATTA CACCAGTCTA 
GTCTTCACAG TGTCAACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCGAAGTGC 
ATTCGAACAT TAACGTCTTC AGGCCAAGTT ACTCTGGGAG AAGCTTGTTC TGCAAGCACC 
AGCCGGACAG TGGCTATTCC TTCCGGAGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACAT TCCTCTACGC GGCCTCTGGA AATGCTGTCA GAATGTGGGA CCTCAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACGGGACAC CTGGGCCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ACGGACAGGA CCTAATCATC ACGGGCTCAA AAGACCACTA CATCAAAATG 
TTTGATGTGA CTGAAGGAGC TCTTGGAACT GTAAGTCCCA CCCACAACTT TGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGTGATA ACTTATTCAG TGGGTCCAGA 
GACAACGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT 
GCACATAAGG ACTGGGTCTG TGCCCTGGGC TTGGTGCCAG GCCACCCGAT TTTGCTTAGT 
GGCTGCAGAG GGGGCATTCT GAAACTCTGG AATGTGGATA CTTTCGTGCC AGTCGGAGAG 
ATGAGAGGCC ATGACAGCCC CATCAATGCC ATAAGTGTTA ACTCCACCCA TGTCTTCACT 
GCAGCCGATG ATCGAACAGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAAATC 
TCTGACACAG GCGATCCGGG GGAGGACAGT ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764056.1
CDS	164..4885
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765834.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765834.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATGTC TGATGAAAGT GACCCCTCTT TGTCGGAGGT ACACAGGGGC 
ATTATCAACC CATTTCCTGC CTGCAGAGGA ATCAGAGCGT CTTTCCTTCA GTGTGTTCAC 
GTAGCTGAGG GGCACACGAA AGCTGTGCTC TGCGTGGATT CGACTGATGA CCTTCTCTTC 
ACTGGATCAA AAGATCGTAC TTGTAAAGTT TGGAATCTCG TGACTGGACA GGAAATTATG 
TCATTAGGGG TTCACCCTAA CAACGTTGTG TCTGTAAAAT ACTGTAATTA CACCAGTCTA 
GTCTTCACAG TGTCAACATC TTATATTAAG GTGTGGGATA TCAGAGAGTC AGCGAAGTGC 
ATTCGAACAT TAACGTCTTC AGGCCAAGTT ACTCTGGGAG AAGCTTGTTC TGCAAGCACC 
AGCCGGACAG TGGCTATTCC TTCCGGAGAG AGCCAGATCA ATCAAATTGC ACTAAACCCA 
ACTGGCACAT TCCTCTACGC GGCCTCTGGA AATGCTGTCA GAATGTGGGA CCTCAAAAGG 
TTTCAGTCTA CAGGAAAGCT AACGGGACAC CTGGGCCCTG TTATGTGCCT TACGGTAGAC 
CAGATCTCCA ACGGACAGGA CCTAATCATC ACGGGCTCAA AAGACCACTA CATCAAAATG 
TTTGATGTGA CTGAAGGAGC TCTTGGAACT GTAAGTCCCA CCCACAACTT TGAGCCTCCT 
CATTATGATG GGATAGAAGC ACTGGCCATT CAAGGTGATA ACTTATTCAG TGGGTCCAGA 
GACAACGGAA TCAAGAAATG GGACTTAGCT CAGAAAGGCC TTCTTCAGCA AGTCCCAAAT 
GCACATAAGG ACTGGGTCTG TGCCCTGGGC TTGGTGCCAG GCCACCCGAT TTTGCTTAGT 
GGCTGCAGAG GGGGCATTCT GAAACTCTGG AATGTGGATA CTTTCGTGCC AGTCGGAGAG 
ATGAGAGGCC ATGACAGCCC CATCAATGCC ATAAGTGTTA ACTCCACCCA TGTCTTCACT 
GCAGCCGATG ATCGAACAGT GAGAATCTGG AAGGCCCACA ACTTGCAAGA TGGTCAAATC 
TCTGACACAG GCGATCCGGG GGAGGACAGT ACCAGTAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776572.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774794.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X38, mRNA.

Price & Availability↑

CloneID	ORa45690
Clone ID Related Accession (Same CDS sequence)	XM_008776572.2 , XM_008765835.2
Accession Version	XM_008776572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGG GCATTATCAA CCCATTTCCT 
GCCTGCAGAG GAATCAGAGC GTCTTTCCTT CAGTGTGTTC ACGTAGCTGA GGGGCACACG 
AAAGCTGTGC TCTGCGTGGA TTCGACTGAT GACCTTCTCT TCACTGGATC AAAAGATCGT 
ACTTGTAAAG TTTGGAATCT CGTGACTGGA CAGGAAATTA TGTCATTAGG GGTTCACCCT 
AACAACGTTG TGTCTGTAAA ATACTGTAAT TACACCAGTC TAGTCTTCAC AGTGTCAACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCGAAGT GCATTCGAAC ATTAACGTCT 
TCAGGCCAAG TTACTCTGGG AGAAGCTTGT TCTGCAAGCA CCAGCCGGAC AGTGGCTATT 
CCTTCCGGAG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC ATTCCTCTAC 
GCGGCCTCTG GAAATGCTGT CAGAATGTGG GACCTCAAAA GGTTTCAGTC TACAGGAAAG 
CTAACGGGAC ACCTGGGCCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAACGGACAG 
GACCTAATCA TCACGGGCTC AAAAGACCAC TACATCAAAA TGTTTGATGT GACTGAAGGA 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTTGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGTGA TAACTTATTC AGTGGGTCCA GAGACAACGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG CCTTCTTCAG CAAGTCCCAA ATGCACATAA GGACTGGGTC 
TGTGCCCTGG GCTTGGTGCC AGGCCACCCG ATTTTGCTTA GTGGCTGCAG AGGGGGCATT 
CTGAAACTCT GGAATGTGGA TACTTTCGTG CCAGTCGGAG AGATGAGAGG CCATGACAGC 
CCCATCAATG CCATAAGTGT TAACTCCACC CATGTCTTCA CTGCAGCCGA TGATCGAACA 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAA TCTCTGACAC AGGCGATCCG 
GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774794.1
CDS	190..4893
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776572.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776572.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGG GCATTATCAA CCCATTTCCT 
GCCTGCAGAG GAATCAGAGC GTCTTTCCTT CAGTGTGTTC ACGTAGCTGA GGGGCACACG 
AAAGCTGTGC TCTGCGTGGA TTCGACTGAT GACCTTCTCT TCACTGGATC AAAAGATCGT 
ACTTGTAAAG TTTGGAATCT CGTGACTGGA CAGGAAATTA TGTCATTAGG GGTTCACCCT 
AACAACGTTG TGTCTGTAAA ATACTGTAAT TACACCAGTC TAGTCTTCAC AGTGTCAACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCGAAGT GCATTCGAAC ATTAACGTCT 
TCAGGCCAAG TTACTCTGGG AGAAGCTTGT TCTGCAAGCA CCAGCCGGAC AGTGGCTATT 
CCTTCCGGAG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC ATTCCTCTAC 
GCGGCCTCTG GAAATGCTGT CAGAATGTGG GACCTCAAAA GGTTTCAGTC TACAGGAAAG 
CTAACGGGAC ACCTGGGCCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAACGGACAG 
GACCTAATCA TCACGGGCTC AAAAGACCAC TACATCAAAA TGTTTGATGT GACTGAAGGA 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTTGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGTGA TAACTTATTC AGTGGGTCCA GAGACAACGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG CCTTCTTCAG CAAGTCCCAA ATGCACATAA GGACTGGGTC 
TGTGCCCTGG GCTTGGTGCC AGGCCACCCG ATTTTGCTTA GTGGCTGCAG AGGGGGCATT 
CTGAAACTCT GGAATGTGGA TACTTTCGTG CCAGTCGGAG AGATGAGAGG CCATGACAGC 
CCCATCAATG CCATAAGTGT TAACTCCACC CATGTCTTCA CTGCAGCCGA TGATCGAACA 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAA TCTCTGACAC AGGCGATCCG 
GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765835.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764057.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ORa45690
Clone ID Related Accession (Same CDS sequence)	XM_008776572.2 , XM_008765835.2
Accession Version	XM_008765835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGG GCATTATCAA CCCATTTCCT 
GCCTGCAGAG GAATCAGAGC GTCTTTCCTT CAGTGTGTTC ACGTAGCTGA GGGGCACACG 
AAAGCTGTGC TCTGCGTGGA TTCGACTGAT GACCTTCTCT TCACTGGATC AAAAGATCGT 
ACTTGTAAAG TTTGGAATCT CGTGACTGGA CAGGAAATTA TGTCATTAGG GGTTCACCCT 
AACAACGTTG TGTCTGTAAA ATACTGTAAT TACACCAGTC TAGTCTTCAC AGTGTCAACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCGAAGT GCATTCGAAC ATTAACGTCT 
TCAGGCCAAG TTACTCTGGG AGAAGCTTGT TCTGCAAGCA CCAGCCGGAC AGTGGCTATT 
CCTTCCGGAG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC ATTCCTCTAC 
GCGGCCTCTG GAAATGCTGT CAGAATGTGG GACCTCAAAA GGTTTCAGTC TACAGGAAAG 
CTAACGGGAC ACCTGGGCCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAACGGACAG 
GACCTAATCA TCACGGGCTC AAAAGACCAC TACATCAAAA TGTTTGATGT GACTGAAGGA 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTTGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGTGA TAACTTATTC AGTGGGTCCA GAGACAACGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG CCTTCTTCAG CAAGTCCCAA ATGCACATAA GGACTGGGTC 
TGTGCCCTGG GCTTGGTGCC AGGCCACCCG ATTTTGCTTA GTGGCTGCAG AGGGGGCATT 
CTGAAACTCT GGAATGTGGA TACTTTCGTG CCAGTCGGAG AGATGAGAGG CCATGACAGC 
CCCATCAATG CCATAAGTGT TAACTCCACC CATGTCTTCA CTGCAGCCGA TGATCGAACA 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAA TCTCTGACAC AGGCGATCCG 
GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764057.1
CDS	164..4867
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSMGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765835.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765835.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGTGTCGCT 
GGGAAAGAGA ATAATGCAGA CCCTGACCAG GAGAAGAAAG AAGAAAAGGG CGTCTCAGAG 
AAAGAAAGCA GTGAGGTGGA CGTGGATGAG AATCAAGAAG CGAGTGACCA TGAGGATGAG 
GAAGATGAGG AAGAGGAAGA CGAGGAAGAA GATGACATCG AAGGAGAAGA AAGCTCTGAT 
GAATCAGATT CCGAATCAGA TGAAAAAGCT AACTATCAGG CTGACCTAGC AAACATCACG 
TGCGAAATTG CGATCAAGCA GAAGCTGATT GACGAACTAG AGAACAGCCA GAAGCGCCTG 
CAGACACTGA AGAAGCAGTA TGAGGAGAAG CTGATGATGC TTCAGCATAA GATCCGGGAC 
ACACAGCTGG AGAGGGACCA GGTTCTCCAG AACCTAGGCT CAGTGGAGTC CTACTCTGAG 
GAAAAAGCCA AAAAGGTGAA GTGCGAATAC GAGAAGAAGC TGCACGCTAT GAACAAAGAG 
CTTCAGCGAC TGCAGACAGC CCAGAAGGAG CACGCCAGGC TGCTCAAAAA CCAGTCTCAG 
TACGAAAAGC AGCTCAAGAA ACTGCAGCAA GACGTCACAG AAATGAAGAA AACCAAGGTT 
CGCCTAATGA AGCAGATGAA AGAAGAGCAA GAGAAGGCCA GGCTGACAGA ATCCAGGAGG 
AACCGGGAAA TCGCTCAGCT CAAAAAGGAT CAGCGCAAAC GAGATCATCA ACTTAGACTT 
CTAGAAGCCC AGAAAAGAAA TCAAGAAGTG GTTCTGCGGC GCAAAACTGA AGAGGTTACA 
GCTCTCCGTA GACAAGTGAG GCCCATGTCT GATAAGGTAG CGGGAAAAGT TACTCGGAAG 
CTGAGCTCAT CCGAGAGCCC CACCCCAGAC GCAGGTTCCA GCGTGACTTC AGGGGAAGCC 
GATGCCTCAC GGCCAGGCAC CCAGCAGAAA ATGAGGATTC CGGTGGCAAG AGTCCAGGCG 
TTACCAACCC CTACAACGAA TGGCACCAGG AAAAGGTATC AGAGGAAAGG ATTCACTGGC 
CGGGTGTTCA CTTCCAAGAC GGCCCGCATG AAGTGGCAGC TGCTGGAGCG CCGGGTGACT 
GAGATCATCA TGCAGAAGAT GACCATTTCC AACATGGAGG CAGACATGAA CAGACTCCTC 
AGGCAACGGG AAGAGCTCAC GAAAAGGCGA GAGAAACTTT CAAAAAGGAG AGAGAAGATA 
GCGAAGGAGA GTGGAGAGGG AGATAAAAAC CTGGTTAACA TCAGCGAGGA GATGGAGTCC 
CTGACCGCGA ACATAGATTA CATCAACGAC AGTATTGCTG ACTGTCAGGC CAACATCATG 
CAGATGGAGG AAGCGAAGGA AGAAGGGGAG ACATTGGATG TCACCGCTGT CATCAATGCC 
TGCACGCTGA CAGAAGCTCG GTACCTGCTA GATCACTTCC TGTCAATGGG CATCAATAAG 
GGTCTTCAGG CTGCCCAGAA AGAGGCTCAG ATTAAAGTCC TGGAAGGTCG ACTCAAGCAA 
ACAGAAATCA CGAGCGCAAC TCAGAACCAG CTCCTGTTCC ATATGCTGAA AGAGAAGGCG 
GAGCTGAACC CGGAGCTCGA TGCGTTGCTA GGCAACGCGC TGCAAGATCT AGATAGCGCC 
CCACTAGAAG ATGAAGAGGA CAGCAGTGAG GAAGATGGCC CTTTACACAG CCCGGGGTCA 
GAGGGAAGCA CGTTGTCTTC GGACCTGATG AAGCTTTGTG GCGAGGTGAA ACCTAAGAAC 
AAGGCTCGAA GGAGAACCAC CACTCAAATG GAGTTGCTGT ATGCAGATAG CAGTGAAGTA 
GCCTCAGACA CTAGTGCAGG AGACGCCTCC TTGCCTGGCC CTCTGACGCC TGTTGCAGAA 
GGGCAAGAGA CTGGAATGAA TACAGAGACA AGTGTCCCTT CTGCTAGGGA CAAAGAGCTT 
CTCCCCCCAT CTGGCTTACC TCCTAAGATA GGCAGCATGG GCATTATCAA CCCATTTCCT 
GCCTGCAGAG GAATCAGAGC GTCTTTCCTT CAGTGTGTTC ACGTAGCTGA GGGGCACACG 
AAAGCTGTGC TCTGCGTGGA TTCGACTGAT GACCTTCTCT TCACTGGATC AAAAGATCGT 
ACTTGTAAAG TTTGGAATCT CGTGACTGGA CAGGAAATTA TGTCATTAGG GGTTCACCCT 
AACAACGTTG TGTCTGTAAA ATACTGTAAT TACACCAGTC TAGTCTTCAC AGTGTCAACA 
TCTTATATTA AGGTGTGGGA TATCAGAGAG TCAGCGAAGT GCATTCGAAC ATTAACGTCT 
TCAGGCCAAG TTACTCTGGG AGAAGCTTGT TCTGCAAGCA CCAGCCGGAC AGTGGCTATT 
CCTTCCGGAG AGAGCCAGAT CAATCAAATT GCACTAAACC CAACTGGCAC ATTCCTCTAC 
GCGGCCTCTG GAAATGCTGT CAGAATGTGG GACCTCAAAA GGTTTCAGTC TACAGGAAAG 
CTAACGGGAC ACCTGGGCCC TGTTATGTGC CTTACGGTAG ACCAGATCTC CAACGGACAG 
GACCTAATCA TCACGGGCTC AAAAGACCAC TACATCAAAA TGTTTGATGT GACTGAAGGA 
GCTCTTGGAA CTGTAAGTCC CACCCACAAC TTTGAGCCTC CTCATTATGA TGGGATAGAA 
GCACTGGCCA TTCAAGGTGA TAACTTATTC AGTGGGTCCA GAGACAACGG AATCAAGAAA 
TGGGACTTAG CTCAGAAAGG CCTTCTTCAG CAAGTCCCAA ATGCACATAA GGACTGGGTC 
TGTGCCCTGG GCTTGGTGCC AGGCCACCCG ATTTTGCTTA GTGGCTGCAG AGGGGGCATT 
CTGAAACTCT GGAATGTGGA TACTTTCGTG CCAGTCGGAG AGATGAGAGG CCATGACAGC 
CCCATCAATG CCATAAGTGT TAACTCCACC CATGTCTTCA CTGCAGCCGA TGATCGAACA 
GTGAGAATCT GGAAGGCCCA CAACTTGCAA GATGGTCAAA TCTCTGACAC AGGCGATCCG 
GGGGAGGACA GTACCAGTAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008776561.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774783.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.

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CloneID	ORa45679
Clone ID Related Accession (Same CDS sequence)	XM_008776561.2 , NM_001371538.1 , XM_008765828.2
Accession Version	XM_008776561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGGGGCATT 
ATCAACCCAT TTCCTGCCTG CAGAGGAATC AGAGCGTCTT TCCTTCAGTG TGTTCACGTA 
GCTGAGGGGC ACACGAAAGC TGTGCTCTGC GTGGATTCGA CTGATGACCT TCTCTTCACT 
GGATCAAAAG ATCGTACTTG TAAAGTTTGG AATCTCGTGA CTGGACAGGA AATTATGTCA 
TTAGGGGTTC ACCCTAACAA CGTTGTGTCT GTAAAATACT GTAATTACAC CAGTCTAGTC 
TTCACAGTGT CAACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC GAAGTGCATT 
CGAACATTAA CGTCTTCAGG CCAAGTTACT CTGGGAGAAG CTTGTTCTGC AAGCACCAGC 
CGGACAGTGG CTATTCCTTC CGGAGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACATTCC TCTACGCGGC CTCTGGAAAT GCTGTCAGAA TGTGGGACCT CAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC GGGACACCTG GGCCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAACG GACAGGACCT AATCATCACG GGCTCAAAAG ACCACTACAT CAAAATGTTT 
GATGTGACTG AAGGAGCTCT TGGAACTGTA AGTCCCACCC ACAACTTTGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGTGATAACT TATTCAGTGG GTCCAGAGAC 
AACGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGCCTTC TTCAGCAAGT CCCAAATGCA 
CATAAGGACT GGGTCTGTGC CCTGGGCTTG GTGCCAGGCC ACCCGATTTT GCTTAGTGGC 
TGCAGAGGGG GCATTCTGAA ACTCTGGAAT GTGGATACTT TCGTGCCAGT CGGAGAGATG 
AGAGGCCATG ACAGCCCCAT CAATGCCATA AGTGTTAACT CCACCCATGT CTTCACTGCA 
GCCGATGATC GAACAGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAAATCTCT 
GACACAGGCG ATCCGGGGGA GGACAGTACC AGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774783.1
CDS	190..5208
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESSREERSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008776561.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776561.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGGGGCATT 
ATCAACCCAT TTCCTGCCTG CAGAGGAATC AGAGCGTCTT TCCTTCAGTG TGTTCACGTA 
GCTGAGGGGC ACACGAAAGC TGTGCTCTGC GTGGATTCGA CTGATGACCT TCTCTTCACT 
GGATCAAAAG ATCGTACTTG TAAAGTTTGG AATCTCGTGA CTGGACAGGA AATTATGTCA 
TTAGGGGTTC ACCCTAACAA CGTTGTGTCT GTAAAATACT GTAATTACAC CAGTCTAGTC 
TTCACAGTGT CAACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC GAAGTGCATT 
CGAACATTAA CGTCTTCAGG CCAAGTTACT CTGGGAGAAG CTTGTTCTGC AAGCACCAGC 
CGGACAGTGG CTATTCCTTC CGGAGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACATTCC TCTACGCGGC CTCTGGAAAT GCTGTCAGAA TGTGGGACCT CAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC GGGACACCTG GGCCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAACG GACAGGACCT AATCATCACG GGCTCAAAAG ACCACTACAT CAAAATGTTT 
GATGTGACTG AAGGAGCTCT TGGAACTGTA AGTCCCACCC ACAACTTTGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGTGATAACT TATTCAGTGG GTCCAGAGAC 
AACGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGCCTTC TTCAGCAAGT CCCAAATGCA 
CATAAGGACT GGGTCTGTGC CCTGGGCTTG GTGCCAGGCC ACCCGATTTT GCTTAGTGGC 
TGCAGAGGGG GCATTCTGAA ACTCTGGAAT GTGGATACTT TCGTGCCAGT CGGAGAGATG 
AGAGGCCATG ACAGCCCCAT CAATGCCATA AGTGTTAACT CCACCCATGT CTTCACTGCA 
GCCGATGATC GAACAGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAAATCTCT 
GACACAGGCG ATCCGGGGGA GGACAGTACC AGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_008765828.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764050.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

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CloneID	ORa45679
Clone ID Related Accession (Same CDS sequence)	XM_008776561.2 , NM_001371538.1 , XM_008765828.2
Accession Version	XM_008765828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGGGGCATT 
ATCAACCCAT TTCCTGCCTG CAGAGGAATC AGAGCGTCTT TCCTTCAGTG TGTTCACGTA 
GCTGAGGGGC ACACGAAAGC TGTGCTCTGC GTGGATTCGA CTGATGACCT TCTCTTCACT 
GGATCAAAAG ATCGTACTTG TAAAGTTTGG AATCTCGTGA CTGGACAGGA AATTATGTCA 
TTAGGGGTTC ACCCTAACAA CGTTGTGTCT GTAAAATACT GTAATTACAC CAGTCTAGTC 
TTCACAGTGT CAACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC GAAGTGCATT 
CGAACATTAA CGTCTTCAGG CCAAGTTACT CTGGGAGAAG CTTGTTCTGC AAGCACCAGC 
CGGACAGTGG CTATTCCTTC CGGAGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACATTCC TCTACGCGGC CTCTGGAAAT GCTGTCAGAA TGTGGGACCT CAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC GGGACACCTG GGCCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAACG GACAGGACCT AATCATCACG GGCTCAAAAG ACCACTACAT CAAAATGTTT 
GATGTGACTG AAGGAGCTCT TGGAACTGTA AGTCCCACCC ACAACTTTGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGTGATAACT TATTCAGTGG GTCCAGAGAC 
AACGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGCCTTC TTCAGCAAGT CCCAAATGCA 
CATAAGGACT GGGTCTGTGC CCTGGGCTTG GTGCCAGGCC ACCCGATTTT GCTTAGTGGC 
TGCAGAGGGG GCATTCTGAA ACTCTGGAAT GTGGATACTT TCGTGCCAGT CGGAGAGATG 
AGAGGCCATG ACAGCCCCAT CAATGCCATA AGTGTTAACT CCACCCATGT CTTCACTGCA 
GCCGATGATC GAACAGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAAATCTCT 
GACACAGGCG ATCCGGGGGA GGACAGTACC AGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764050.1
CDS	164..5182
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESSREERSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_008765828.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765828.2

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGGGGCATT 
ATCAACCCAT TTCCTGCCTG CAGAGGAATC AGAGCGTCTT TCCTTCAGTG TGTTCACGTA 
GCTGAGGGGC ACACGAAAGC TGTGCTCTGC GTGGATTCGA CTGATGACCT TCTCTTCACT 
GGATCAAAAG ATCGTACTTG TAAAGTTTGG AATCTCGTGA CTGGACAGGA AATTATGTCA 
TTAGGGGTTC ACCCTAACAA CGTTGTGTCT GTAAAATACT GTAATTACAC CAGTCTAGTC 
TTCACAGTGT CAACATCTTA TATTAAGGTG TGGGATATCA GAGAGTCAGC GAAGTGCATT 
CGAACATTAA CGTCTTCAGG CCAAGTTACT CTGGGAGAAG CTTGTTCTGC AAGCACCAGC 
CGGACAGTGG CTATTCCTTC CGGAGAGAGC CAGATCAATC AAATTGCACT AAACCCAACT 
GGCACATTCC TCTACGCGGC CTCTGGAAAT GCTGTCAGAA TGTGGGACCT CAAAAGGTTT 
CAGTCTACAG GAAAGCTAAC GGGACACCTG GGCCCTGTTA TGTGCCTTAC GGTAGACCAG 
ATCTCCAACG GACAGGACCT AATCATCACG GGCTCAAAAG ACCACTACAT CAAAATGTTT 
GATGTGACTG AAGGAGCTCT TGGAACTGTA AGTCCCACCC ACAACTTTGA GCCTCCTCAT 
TATGATGGGA TAGAAGCACT GGCCATTCAA GGTGATAACT TATTCAGTGG GTCCAGAGAC 
AACGGAATCA AGAAATGGGA CTTAGCTCAG AAAGGCCTTC TTCAGCAAGT CCCAAATGCA 
CATAAGGACT GGGTCTGTGC CCTGGGCTTG GTGCCAGGCC ACCCGATTTT GCTTAGTGGC 
TGCAGAGGGG GCATTCTGAA ACTCTGGAAT GTGGATACTT TCGTGCCAGT CGGAGAGATG 
AGAGGCCATG ACAGCCCCAT CAATGCCATA AGTGTTAACT CCACCCATGT CTTCACTGCA 
GCCGATGATC GAACAGTGAG AATCTGGAAG GCCCACAACT TGCAAGATGG TCAAATCTCT 
GACACAGGCG ATCCGGGGGA GGACAGTACC AGTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017595289.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450778.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

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CloneID	ORa55793
Clone ID Related Accession (Same CDS sequence)	XM_017595289.1 , XM_017603483.1
Accession Version	XM_017595289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450778.1
CDS	164..5194
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESSREERSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017595289.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595289.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21a ( XM_017603483.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017458972.1 Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.

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CloneID	ORa55793
Clone ID Related Accession (Same CDS sequence)	XM_017595289.1 , XM_017603483.1
Accession Version	XM_017603483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21A isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017458972.1
CDS	190..5220
Translation	MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVKHLFKSIEEKKTSAIKNGVPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENVTDNKMVSESPQMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKSLTRATARSPFFSASSAFSSTILSSDKETIEIIDLAKKDLEKLKRKEKRKKKRLQKLEESSREERSVAGKENNADPDQEKKEEKGVSEKESSEVDVDENQEASDHEDEEDEEEEDEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVTEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRKLSSSESPTPDAGSSVTSGEADASRPGTQQKMRIPVARVQALPTPTTNGTRKRYQRKGFTGRVFTSKTARMKWQLLERRVTEIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIAKESGEGDKNLVNISEEMESLTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGNALQDLDSAPLEDEEDSSEEDGPLHSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLPGPLTPVAEGQETGMNTETSVPSARDKELLPPSGLPPKIGSISRQPSVSEKKVPEPSPVTRRKAYEKADKAKTKEQKHSDSGASETSLSPPSSPPSRPRNELNVFNRLTVSQGSAPVQQDKSDESDPSLSEVHRSSRRGIINPFPACRGIRASFLQCVHVAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEACSASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQQVPNAHKDWVCALGLVPGHPILLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAISVNSTHVFTAADDRTVRIWKAHNLQDGQISDTGDPGEDSTSN

Target ORF information:

RefSeq Version	XM_017603483.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21A (Kif21a), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603483.1

ORF Insert Sequence:

ATGTTGGGCG CCGCGGACGA GAGCTCGGTG CGCGTGGCTG TCAGAATCAG ACCACAGCTT 
GCCAAAGAAA AGATTGAAGG CTGCCATATC TGCACGTCAG TCACACCAGG GGAGCCTCAG 
GTCTTCCTAG GGAAAGATAA GGCCTTTACT TTTGATTATG TGTTCGACAT CGATTCCCAG 
CAGGAGCAGA TCTACACTCA GTGCATTGAA AAACTCATCG AAGGCTGTTT CGAAGGATAC 
AATGCTACGG TGTTTGCGTA TGGACAAACC GGAGCAGGGA AGACGTACAC GATGGGAACC 
GGATTTGACG TCAACATCAT GGAGGAAGAG CAAGGCATCA TCTCTCGCGC TGTTAAACAC 
CTTTTCAAGA GTATTGAAGA GAAAAAGACC TCAGCGATTA AAAACGGGGT CCCCCCTCCT 
GAATTCAAAG TGAACGCTCA GTTCCTAGAG CTCTATAATG AAGAGGTCCT TGACTTGTTT 
GACACCACCC GGGATATTGA TGCAAAAAAT AAAAAGTCAA ATATAAGAAT TCACGAAGAT 
TCGACTGGCG GGATTTATAC TGTGGGAGTC ACAACACGCA CTGTGAATAC AGAACCAGAG 
ATGATGCAGT GTCTGAAGCT GGGCGCTCTC TCGCGCACCA CGGCGAGTAC CCAGATGAAT 
GTGCAGAGCT CGCGCTCCCA TGCCATTTTT ACCATTCACG TGTGTCAAAC CAGGGTGTGT 
CCCCAAACAG ACGCTGAGAA CGTAACCGAT AATAAGATGG TGTCCGAATC CCCGCAGATG 
AATGAGTTTG AAACGCTGAC CGCGAAGTTT CACTTCGTTG ACCTGGCAGG ATCGGAAAGG 
TTGAAGAGAA CCGGAGCTAC AGGCGAGAGG GCAAAAGAGG GCATTTCCAT CAACTGCGGG 
CTTTTGGCTC TTGGAAATGT CATCAGTGCC TTGGGGGACA AGAGCAAGAG AGCCACCCAC 
GTCCCTTACA GAGACTCTAA ACTGACAAGA CTCTTGCAGG ATTCCCTTGG GGGGAACAGC 
CAAACAATCA TGATAGCGTG TGTCAGCCCT TCAGACCGGG ATTTTATGGA GACACTAAAC 
ACTCTGAAAT ACGCCAACCG AGCTAGAAAT ATTAAGAACA AGGTGATGGT CAATCAAGAC 
AGAGCCAGTC AGCAAATCAA TGCTCTCCGA AGTGAGATCA CGCGGCTTCA GATGGAGCTC 
ATGGAATACA AAACAGGTAA AAGAATCATC GATGAGGAAG GCGTAGAAAG CATCAACGAC 
ATGTTTCATG AGAATGCTAT GCTCCAGACG GAAAATAATA ATCTACGTGT AAGAATAAAA 
GCCATGCAGG AGACCGTTGA TGCGCTGAGG GCCAGAATCA CGCAGCTTGT CAGTGAGCAG 
GCCAACCAAG TTCTTGCCCG AGCAGGTGAA GGGAATGAAG AGATTAGTAA TATGATTCAC 
AGTTACATCA AAGAAATCGA AGATCTCAGG GCAAAATTAT TAGAAAGCGA GGCAGTGAAC 
GAGAACCTTC GGAAGAGCTT GACCAGAGCC ACGGCGAGAT CTCCTTTCTT CAGCGCGTCG 
TCCGCTTTCT CGTCGACCAT ACTGTCTTCA GACAAAGAAA CCATCGAAAT TATAGATCTG 
GCGAAGAAAG ATTTGGAGAA GCTGAAGAGG AAGGAGAAGA GGAAGAAGAA GAGGCTACAG 
AAACTTGAGG AAAGCAGTCG AGAAGAAAGA AGTGTCGCTG GGAAAGAGAA TAATGCAGAC 
CCTGACCAGG AGAAGAAAGA AGAAAAGGGC GTCTCAGAGA AAGAAAGCAG TGAGGTGGAC 
GTGGATGAGA ATCAAGAAGC GAGTGACCAT GAGGATGAGG AAGATGAGGA AGAGGAAGAC 
GAGGAAGAAG ATGACATCGA AGGAGAAGAA AGCTCTGATG AATCAGATTC CGAATCAGAT 
GAAAAAGCTA ACTATCAGGC TGACCTAGCA AACATCACGT GCGAAATTGC GATCAAGCAG 
AAGCTGATTG ACGAACTAGA GAACAGCCAG AAGCGCCTGC AGACACTGAA GAAGCAGTAT 
GAGGAGAAGC TGATGATGCT TCAGCATAAG ATCCGGGACA CACAGCTGGA GAGGGACCAG 
GTTCTCCAGA ACCTAGGCTC AGTGGAGTCC TACTCTGAGG AAAAAGCCAA AAAGGTGAAG 
TGCGAATACG AGAAGAAGCT GCACGCTATG AACAAAGAGC TTCAGCGACT GCAGACAGCC 
CAGAAGGAGC ACGCCAGGCT GCTCAAAAAC CAGTCTCAGT ACGAAAAGCA GCTCAAGAAA 
CTGCAGCAAG ACGTCACAGA AATGAAGAAA ACCAAGGTTC GCCTAATGAA GCAGATGAAA 
GAAGAGCAAG AGAAGGCCAG GCTGACAGAA TCCAGGAGGA ACCGGGAAAT CGCTCAGCTC 
AAAAAGGATC AGCGCAAACG AGATCATCAA CTTAGACTTC TAGAAGCCCA GAAAAGAAAT 
CAAGAAGTGG TTCTGCGGCG CAAAACTGAA GAGGTTACAG CTCTCCGTAG ACAAGTGAGG 
CCCATGTCTG ATAAGGTAGC GGGAAAAGTT ACTCGGAAGC TGAGCTCATC CGAGAGCCCC 
ACCCCAGACG CAGGTTCCAG CGTGACTTCA GGGGAAGCCG ATGCCTCACG GCCAGGCACC 
CAGCAGAAAA TGAGGATTCC GGTGGCAAGA GTCCAGGCGT TACCAACCCC TACAACGAAT 
GGCACCAGGA AAAGGTATCA GAGGAAAGGA TTCACTGGCC GGGTGTTCAC TTCCAAGACG 
GCCCGCATGA AGTGGCAGCT GCTGGAGCGC CGGGTGACTG AGATCATCAT GCAGAAGATG 
ACCATTTCCA ACATGGAGGC AGACATGAAC AGACTCCTCA GGCAACGGGA AGAGCTCACG 
AAAAGGCGAG AGAAACTTTC AAAAAGGAGA GAGAAGATAG CGAAGGAGAG TGGAGAGGGA 
GATAAAAACC TGGTTAACAT CAGCGAGGAG ATGGAGTCCC TGACCGCGAA CATAGATTAC 
ATCAACGACA GTATTGCTGA CTGTCAGGCC AACATCATGC AGATGGAGGA AGCGAAGGAA 
GAAGGGGAGA CATTGGATGT CACCGCTGTC ATCAATGCCT GCACGCTGAC AGAAGCTCGG 
TACCTGCTAG ATCACTTCCT GTCAATGGGC ATCAATAAGG GTCTTCAGGC TGCCCAGAAA 
GAGGCTCAGA TTAAAGTCCT GGAAGGTCGA CTCAAGCAAA CAGAAATCAC GAGCGCAACT 
CAGAACCAGC TCCTGTTCCA TATGCTGAAA GAGAAGGCGG AGCTGAACCC GGAGCTCGAT 
GCGTTGCTAG GCAACGCGCT GCAAGATCTA GATAGCGCCC CACTAGAAGA TGAAGAGGAC 
AGCAGTGAGG AAGATGGCCC TTTACACAGC CCGGGGTCAG AGGGAAGCAC GTTGTCTTCG 
GACCTGATGA AGCTTTGTGG CGAGGTGAAA CCTAAGAACA AGGCTCGAAG GAGAACCACC 
ACTCAAATGG AGTTGCTGTA TGCAGATAGC AGTGAAGTAG CCTCAGACAC TAGTGCAGGA 
GACGCCTCCT TGCCTGGCCC TCTGACGCCT GTTGCAGAAG GGCAAGAGAC TGGAATGAAT 
ACAGAGACAA GTGTCCCTTC TGCTAGGGAC AAAGAGCTTC TCCCCCCATC TGGCTTACCT 
CCTAAGATAG GCAGCATTTC CAGGCAGCCA TCTGTATCAG AAAAAAAAGT ACCAGAGCCT 
TCCCCTGTAA CAAGGAGAAA GGCATATGAG AAAGCAGACA AAGCAAAGAC CAAGGAGCAG 
AAACACTCAG ATTCTGGCGC TTCCGAGACT AGCCTCTCAC CTCCTTCCTC CCCACCAAGC 
CGGCCCCGTA ACGAACTGAA TGTGTTTAAT CGCCTTACCG TTTCTCAGGG AAGTGCGCCA 
GTTCAGCAGG ATAAGTCTGA TGAAAGTGAC CCCTCTTTGT CGGAGGTACA CAGATCCTCC 
AGAAGGGGCA TTATCAACCC ATTTCCTGCC TGCAGAGGAA TCAGAGCGTC TTTCCTTCAG 
TGTGTTCACG TAGCTGAGGG GCACACGAAA GCTGTGCTCT GCGTGGATTC GACTGATGAC 
CTTCTCTTCA CTGGATCAAA AGATCGTACT TGTAAAGTTT GGAATCTCGT GACTGGACAG 
GAAATTATGT CATTAGGGGT TCACCCTAAC AACGTTGTGT CTGTAAAATA CTGTAATTAC 
ACCAGTCTAG TCTTCACAGT GTCAACATCT TATATTAAGG TGTGGGATAT CAGAGAGTCA 
GCGAAGTGCA TTCGAACATT AACGTCTTCA GGCCAAGTTA CTCTGGGAGA AGCTTGTTCT 
GCAAGCACCA GCCGGACAGT GGCTATTCCT TCCGGAGAGA GCCAGATCAA TCAAATTGCA 
CTAAACCCAA CTGGCACATT CCTCTACGCG GCCTCTGGAA ATGCTGTCAG AATGTGGGAC 
CTCAAAAGGT TTCAGTCTAC AGGAAAGCTA ACGGGACACC TGGGCCCTGT TATGTGCCTT 
ACGGTAGACC AGATCTCCAA CGGACAGGAC CTAATCATCA CGGGCTCAAA AGACCACTAC 
ATCAAAATGT TTGATGTGAC TGAAGGAGCT CTTGGAACTG TAAGTCCCAC CCACAACTTT 
GAGCCTCCTC ATTATGATGG GATAGAAGCA CTGGCCATTC AAGGTGATAA CTTATTCAGT 
GGGTCCAGAG ACAACGGAAT CAAGAAATGG GACTTAGCTC AGAAAGGCCT TCTTCAGCAA 
GTCCCAAATG CACATAAGGA CTGGGTCTGT GCCCTGGGCT TGGTGCCAGG CCACCCGATT 
TTGCTTAGTG GCTGCAGAGG GGGCATTCTG AAACTCTGGA ATGTGGATAC TTTCGTGCCA 
GTCGGAGAGA TGAGAGGCCA TGACAGCCCC ATCAATGCCA TAAGTGTTAA CTCCACCCAT 
GTCTTCACTG CAGCCGATGA TCGAACAGTG AGAATCTGGA AGGCCCACAA CTTGCAAGAT 
GGTCAAATCT CTGACACAGG CGATCCGGGG GAGGACAGTA CCAGTAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).
Nature genetics35(4)318-21(2003 Dec)
Yamada K,Andrews C,Chan WM,McKeown CA,Magli A,de Berardinis T,Loewenstein A,Lazar M,O'Keefe M,Letson R,London A,Ruttum M,Matsumoto N,Saito N,Morris L,Del Monte M,Johnson RH,Uyama E,Houtman WA,de Vries B,Carlow TJ,Hart BL,Krawiecki N,Shoffner J,Vogel MC,Katowitz J,Goldstein SM,Levin AV,Sener EC,Ozturk BT,Akarsu AN,Brodsky MC,Hanisch F,Cruse RP,Zubcov AA,Robb RM,Roggenk?emper P,Gottlob I,Kowal L,Battu R,Traboulsi EI,Franceschini P,Newlin A,Demer JL,Engle EC

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Kif21a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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