sfi1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012969720.2	XP_012825174.1	protein SFI1 homolog isoform X5
XM_012969720.3	XP_012825174.2	protein SFI1 homolog isoform X5
XM_004910593.3	XP_004910650.1	protein SFI1 homolog isoform X1
XM_012969717.2	XP_012825171.1	protein SFI1 homolog isoform X4
XM_004910593.4	XP_004910650.2	protein SFI1 homolog isoform X1
XM_012969717.3	XP_012825171.2	protein SFI1 homolog isoform X4
XM_002931922.4	XP_002931968.2	protein SFI1 homolog isoform X2
XM_004910594.4	XP_004910651.2	protein SFI1 homolog isoform X3
XM_004910594.3	XP_004910651.1	protein SFI1 homolog isoform X3
XM_018090116.1	XP_017945605.1	protein SFI1 homolog isoform X6
XM_002931922.5	XP_002931968.3	protein SFI1 homolog isoform X2
XM_018090116.2	XP_017945605.2	protein SFI1 homolog isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42154	XM_018090116.1 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa48881	XM_004910593.4 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa48882	XM_002931922.5 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa48883	XM_004910594.4 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa48884	XM_012969717.3 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa48885	XM_012969720.3 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa48886	XM_018090116.2 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa03646	XM_004910593.3 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03647	XM_002931922.4 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa03648	XM_004910594.3 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa33152	XM_012969717.2 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa33153	XM_012969720.2 Latest version!	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

sfi1 ( XM_018090116.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945605.1 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa42154
Clone ID Related Accession (Same CDS sequence)	XM_018090116.1
Accession Version	XM_018090116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGA AACTTAATAT CAGAGCTGAG TGCCACAACA GGTATGTCTT ATACAAAATG 
TGTTTCTATG CCTGGAGGAA ATATATTTCA TTGCAGCAGA GGAAAAAGTT CAAGGTTCAG 
GCTGCAGAGA TCCATGCACA GACTCATTTA AAGCATCAGG CACTTCACCA TTGGAAAAAT 
TATGTGAAAA TACGCAGAAC CAAGCAGCGT ATGCTGTTAA TTGCGCTGGA ATTCAGAGAA 
CAACAAGACC TTAGAAACTC CTGGTACATG TGGGTACTAC GGTTAGAGCA GCAACAGAGC 
TACCGTGATA TGGACATGCT GGCTTTAAAT CACTGGGCTG TGAGCCTGCA AAACAGGGCT 
TGGCTGCAGT GGAGAGAGAT TTATGTACAT AGAATATCTG AGAAGAAAAA GGTTTTGCAA 
GCAGTAAAGC ATAATCGAAA ATGCAAGTTA CAAGCGGCGC TGAAAGCCTG GCAGCTATAC 
TTATGTTATC GTCGAGAGAA ACGACGGCAT CAAATGATGG CTGTTCGTTT GCATCAGAAT 
TTTCTAACAA AGAGATACTT CTTATACTGG CACCACGCAT TGGAGAAAGT TTGGAGTATG 
CACAGAATAG AAAAACATAT TGAGGCTTTG GCTGTACGCT GCCTGCTGCG AAGGACTCTA 
ACACATTGGA AAAATTACAT GATGGTACAT TCCGAGAATA TTGGGCTGCA AAAAGTAGCA 
GAAGCGTATC ACAGAAACAA CATAATGAAA TTAGGATTCC ACGCCCTGAA AAATAATATA 
AACCAAGTTC GCATATATCA ACAACAAAGT CTTCAGGCTG TCCAGCAATA CCAAATTATG 
CTTATCCATA AGTACTGGAC TGTATGGAAG TTACGCTTGG AACAGAAAGA GGAAGAAAAA 
CTTTTGACAT TGACATTAAC AGCACATGCT CATTACAGGT CGGTGTTAAT ACAGAAGTCT 
TTCAGGGTGT GGTTACAGTA CAGACAGAAA AGGAAACTGA AGCAGGTGCT AAATAAAACA 
GCGTATAGTC ACTATGCAAA ATCCTTATTG CCTCACAGCT TTCAAGTGTG GAGGAAACAT 
CAAGAACATC AATGGCAGAA AAGACACATG GTAGAAAAAG CAACACAGTT TCACAGGTAT 
ACTGTACAGA GAAGAGTACT CTTCACATGG CATAAGGAGT TAAGCCAACA GAGGGAATAT 
CGCCTGAGTG AGAGAATGGC TATACTACAT TTTAACTGGA GGCTATTAGA ACAATGTTGG 
TACTCCTGGA AAAATCAGTT AGTTCTCCAC ATAGCAGAAA AAAAAGGCAA TTCTGAAGCT 
GCTGAACATC ACAGGAGACA TCAGTTGCTA AATGCCATGA CCATATGGAA GGAACACGTA 
CTGGAGATAA AGGCAGAGAG AGCTCGAGAG GAGAAAGCCA TACTTCACCA CCGTGTGCTT 
TGCATGCAGA ATGCCTGGAA CCACTGGAAA TGGTTTTTGT TTCAGAGACA TGAAAAGTGG 
AAAAAACAAG TCAGGGCTGA TCTACACTAC CAGCGCAACT TGCTTGCAAA TGTATTTCAG 
GCATGGAAAC ATTATCATAT TAGTGTACAG GACATACTGC ATCAGGTGAA GGAGAAAGAG 
AAGCAAAAAA ATGAATCTTT CTTGCGAGGT GCACTGTGTA GCTGGAGGAA TCAAGCATCT 
GCTCAGGTTC TGGAGAGAAG GCAGGAAAGA CTTGCAATTA TTCACTACAG GGCTACAACT 
CAAATAAAGG TTGTTCGGGC ATGGAGAGAT TCTGCAGCTG TGCTGGCACA TTGTAGGCAG 
AAAAAGGCAG AGGAAGTGAA AGAGGCAATG CTTTTTCTAC AGAAAAGAAA GCTTCAGTGT 
TTCTTTATGC ACTGGAGGCA ACTAAGTTAC ACAACAAGGG ACCTTCGTGT TAGAATGGAG 
GCAGCCGCAA CGCATCATGT AAAAAATTTG CTGAAGAATT GCATGACAAA ATGGAAAGCT 
TATCGTGCTC ACTGCTTACG GAAAATGCTA CTCCAGCGTC AAGGAGCCTG GTTTGCAGAA 
CAGAGACTTA GCCGAAATTG TCTTAGACAG TGGTGCCAAA TGCTGGTAGA AAAGCAACAG 
CAGGACAAGA AGACTGTGAA AGCTTTATGG AACTGGTCCC TTACACTGCA GGGAAAGGTG 
TTTGATGGCT GGCTATCTTA TGTCTGTGAG CGTCGGCGAA AAAAGGATCG CATTGTAGCA 
GCGGTAGAAC TGTATCGTGC AGATCTACTG CAAACTGGAG TGACCAGAAT CCTAAATTAT 
GCATCTGGCA TGAAACAATT CAGATCTCAT CTGAACACTC AGCACCAAGT AAAGGAAGTG 
CAGATGCAAA ATGCCGCAGC TCGTCGATGT GCCATAATCT GGAAGGAGAA GGTCTTCAGA 
AGGCTCCATC AAAAAACTTC TCAGAAGAGG GTGACTTTCA GTACTTCGAA AGCTGATTCA 
TTATCTACAG AAGAAGAGAC CTATTCAACA AAGACCTCAA GGGAAATAAT GTGTGCCGTA 
CCCTTGACTA GTGGGGAGCC CACGCTAAGT GTAATTAGCA AGTTGCGAGC AGAGAGAACA 
AAACCCCGCA GCCCTGAGTT CCTGCTGCAG TCACTGGAGA GAGAGGGACT ACTGAATGCT 
GTGGTCAGCA GCTGCAACAC AAGAAAAGAT TCAGACGAAG AGTCCTTGGC CTTGAAAATG 
CCTCTACAGA AAGTCCAAGC AACAAATCCT ACCAAACAAC TTCCTGGTCT GCCTCTGCAA 
CACACATGCA CTTCTTGTGT AACAAAGGAG TCCGCTGCTG CAATTCAGCT TCCTCCTACC 
CTAGGTTCTG CAGCATCATC AGAACCTCAT CAGTTGATCC CAAACCAGGC TTCATCTTGT 
TCAACACTAA TGCCTCCATC TTCTTTTGTT CCTCTGGTTC AAAGCAAGAT AGACATAGCA 
AAAAGTGCTC TTCCACACAT TAGAGAACAG CCTCAGATAA AGAACCTCTC CAGCACAGAA 
TTCTCCGATT TTTCATGTCA GCTACTGTCA CCAACTGATT TCCTTCAAGG CATTGGGCCC 
CAGGCCCAAA GTGGTACTAC TGCAATTCAA ATGGGAAAAA GTCCAGAGAA ACCAAAAGAG 
AGCAAACAGA CTGATACCCT TAAAAATGAA CTTCTAGAGA TTCAAAGAAT TATGCAGCAA 
TACCAAGACC AGAAAGAGGA GCTCAAGGAT TGGCGCAGAC ATGCAGGAGT GCTACGCAGG 
TGGCTGAAAA CCAGTGACCA TGTGCTAGAC CCTTATGAAC AATCACTCAG AAACGAAGTG 
CAGACTGAAC TGCAGCAGCT CGAAGTGAAG ATTGAAGAGC ACTCACAGCT GCTAATCTCA 
AACAAGAGAC AAATGCAGCT TTATATTAGT CGCCTTCAGG AGATCAGAGC ATCAATGGAC 
TTATTGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945605.1
CDS	446..3877
Translation	MEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIGLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_018090116.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090116.1

ORF Insert Sequence:

ATGGAGTGGA AACTTAATAT CAGAGCTGAG TGCCACAACA GGTATGTCTT ATACAAAATG 
TGTTTCTATG CCTGGAGGAA ATATATTTCA TTGCAGCAGA GGAAAAAGTT CAAGGTTCAG 
GCTGCAGAGA TCCATGCACA GACTCATTTA AAGCATCAGG CACTTCACCA TTGGAAAAAT 
TATGTGAAAA TACGCAGAAC CAAGCAGCGT ATGCTGTTAA TTGCGCTGGA ATTCAGAGAA 
CAACAAGACC TTAGAAACTC CTGGTACATG TGGGTACTAC GGTTAGAGCA GCAACAGAGC 
TACCGTGATA TGGACATGCT GGCTTTAAAT CACTGGGCTG TGAGCCTGCA AAACAGGGCT 
TGGCTGCAGT GGAGAGAGAT TTATGTACAT AGAATATCTG AGAAGAAAAA GGTTTTGCAA 
GCAGTAAAGC ATAATCGAAA ATGCAAGTTA CAAGCGGCGC TGAAAGCCTG GCAGCTATAC 
TTATGTTATC GTCGAGAGAA ACGACGGCAT CAAATGATGG CTGTTCGTTT GCATCAGAAT 
TTTCTAACAA AGAGATACTT CTTATACTGG CACCACGCAT TGGAGAAAGT TTGGAGTATG 
CACAGAATAG AAAAACATAT TGAGGCTTTG GCTGTACGCT GCCTGCTGCG AAGGACTCTA 
ACACATTGGA AAAATTACAT GATGGTACAT TCCGAGAATA TTGGGCTGCA AAAAGTAGCA 
GAAGCGTATC ACAGAAACAA CATAATGAAA TTAGGATTCC ACGCCCTGAA AAATAATATA 
AACCAAGTTC GCATATATCA ACAACAAAGT CTTCAGGCTG TCCAGCAATA CCAAATTATG 
CTTATCCATA AGTACTGGAC TGTATGGAAG TTACGCTTGG AACAGAAAGA GGAAGAAAAA 
CTTTTGACAT TGACATTAAC AGCACATGCT CATTACAGGT CGGTGTTAAT ACAGAAGTCT 
TTCAGGGTGT GGTTACAGTA CAGACAGAAA AGGAAACTGA AGCAGGTGCT AAATAAAACA 
GCGTATAGTC ACTATGCAAA ATCCTTATTG CCTCACAGCT TTCAAGTGTG GAGGAAACAT 
CAAGAACATC AATGGCAGAA AAGACACATG GTAGAAAAAG CAACACAGTT TCACAGGTAT 
ACTGTACAGA GAAGAGTACT CTTCACATGG CATAAGGAGT TAAGCCAACA GAGGGAATAT 
CGCCTGAGTG AGAGAATGGC TATACTACAT TTTAACTGGA GGCTATTAGA ACAATGTTGG 
TACTCCTGGA AAAATCAGTT AGTTCTCCAC ATAGCAGAAA AAAAAGGCAA TTCTGAAGCT 
GCTGAACATC ACAGGAGACA TCAGTTGCTA AATGCCATGA CCATATGGAA GGAACACGTA 
CTGGAGATAA AGGCAGAGAG AGCTCGAGAG GAGAAAGCCA TACTTCACCA CCGTGTGCTT 
TGCATGCAGA ATGCCTGGAA CCACTGGAAA TGGTTTTTGT TTCAGAGACA TGAAAAGTGG 
AAAAAACAAG TCAGGGCTGA TCTACACTAC CAGCGCAACT TGCTTGCAAA TGTATTTCAG 
GCATGGAAAC ATTATCATAT TAGTGTACAG GACATACTGC ATCAGGTGAA GGAGAAAGAG 
AAGCAAAAAA ATGAATCTTT CTTGCGAGGT GCACTGTGTA GCTGGAGGAA TCAAGCATCT 
GCTCAGGTTC TGGAGAGAAG GCAGGAAAGA CTTGCAATTA TTCACTACAG GGCTACAACT 
CAAATAAAGG TTGTTCGGGC ATGGAGAGAT TCTGCAGCTG TGCTGGCACA TTGTAGGCAG 
AAAAAGGCAG AGGAAGTGAA AGAGGCAATG CTTTTTCTAC AGAAAAGAAA GCTTCAGTGT 
TTCTTTATGC ACTGGAGGCA ACTAAGTTAC ACAACAAGGG ACCTTCGTGT TAGAATGGAG 
GCAGCCGCAA CGCATCATGT AAAAAATTTG CTGAAGAATT GCATGACAAA ATGGAAAGCT 
TATCGTGCTC ACTGCTTACG GAAAATGCTA CTCCAGCGTC AAGGAGCCTG GTTTGCAGAA 
CAGAGACTTA GCCGAAATTG TCTTAGACAG TGGTGCCAAA TGCTGGTAGA AAAGCAACAG 
CAGGACAAGA AGACTGTGAA AGCTTTATGG AACTGGTCCC TTACACTGCA GGGAAAGGTG 
TTTGATGGCT GGCTATCTTA TGTCTGTGAG CGTCGGCGAA AAAAGGATCG CATTGTAGCA 
GCGGTAGAAC TGTATCGTGC AGATCTACTG CAAACTGGAG TGACCAGAAT CCTAAATTAT 
GCATCTGGCA TGAAACAATT CAGATCTCAT CTGAACACTC AGCACCAAGT AAAGGAAGTG 
CAGATGCAAA ATGCCGCAGC TCGTCGATGT GCCATAATCT GGAAGGAGAA GGTCTTCAGA 
AGGCTCCATC AAAAAACTTC TCAGAAGAGG GTGACTTTCA GTACTTCGAA AGCTGATTCA 
TTATCTACAG AAGAAGAGAC CTATTCAACA AAGACCTCAA GGGAAATAAT GTGTGCCGTA 
CCCTTGACTA GTGGGGAGCC CACGCTAAGT GTAATTAGCA AGTTGCGAGC AGAGAGAACA 
AAACCCCGCA GCCCTGAGTT CCTGCTGCAG TCACTGGAGA GAGAGGGACT ACTGAATGCT 
GTGGTCAGCA GCTGCAACAC AAGAAAAGAT TCAGACGAAG AGTCCTTGGC CTTGAAAATG 
CCTCTACAGA AAGTCCAAGC AACAAATCCT ACCAAACAAC TTCCTGGTCT GCCTCTGCAA 
CACACATGCA CTTCTTGTGT AACAAAGGAG TCCGCTGCTG CAATTCAGCT TCCTCCTACC 
CTAGGTTCTG CAGCATCATC AGAACCTCAT CAGTTGATCC CAAACCAGGC TTCATCTTGT 
TCAACACTAA TGCCTCCATC TTCTTTTGTT CCTCTGGTTC AAAGCAAGAT AGACATAGCA 
AAAAGTGCTC TTCCACACAT TAGAGAACAG CCTCAGATAA AGAACCTCTC CAGCACAGAA 
TTCTCCGATT TTTCATGTCA GCTACTGTCA CCAACTGATT TCCTTCAAGG CATTGGGCCC 
CAGGCCCAAA GTGGTACTAC TGCAATTCAA ATGGGAAAAA GTCCAGAGAA ACCAAAAGAG 
AGCAAACAGA CTGATACCCT TAAAAATGAA CTTCTAGAGA TTCAAAGAAT TATGCAGCAA 
TACCAAGACC AGAAAGAGGA GCTCAAGGAT TGGCGCAGAC ATGCAGGAGT GCTACGCAGG 
TGGCTGAAAA CCAGTGACCA TGTGCTAGAC CCTTATGAAC AATCACTCAG AAACGAAGTG 
CAGACTGAAC TGCAGCAGCT CGAAGTGAAG ATTGAAGAGC ACTCACAGCT GCTAATCTCA 
AACAAGAGAC AAATGCAGCT TTATATTAGT CGCCTTCAGG AGATCAGAGC ATCAATGGAC 
TTATTGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_004910593.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910650.2 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa48881
Clone ID Related Accession (Same CDS sequence)	XM_004910593.4
Accession Version	XM_004910593.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910593.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC AGCTGCAACA CAAGAAAAGA TTCAGACGAA 
GAGTCCTTGG CCTTGAAAAT GCCTCTACAG AAAGTCCAAG CAACAAATCC TACCAAACAA 
CTTCCTGGTC TGCCTCTGCA ACACACATGC ACTTCTTGTG TAACAAAGGA GTCCGCTGCT 
GCAATTCAGC TTCCTCCTAC CCTAGGTTCT GCAGCATCAT CAGAACCTCA TCAGTTGATC 
CCAAACCAGG CTTCATCTTG TTCAACACTA ATGCCTCCAT CTTCTTTTGT TCCTCTGGTT 
CAAAGCAAGA TAGACATAGC AAAAAGTGCT CTTCCACACA TTAGAGAACA GCCTCAGATA 
AAGAACCTCT CCAGCACAGA ATTCTCCGAT TTTTCATGTC AGCTACTGTC ACCAACTGAT 
TTCCTTCAAG GCATTGGGCC CCAGGCCCAA AGTGGTACTA CTGCAATTCA AATGGGAAAA 
AGTCCAGAGA AACCAAAAGA GAGCAAACAG ACTGATACCC TTAAAAATGA ACTTCTAGAG 
ATTCAAAGAA TTATGCAGCA ATACCAAGAC CAGAAAGAGG AGCTCAAGGA TTGGCGCAGA 
CATGCAGGAG TGCTACGCAG GTGGCTGAAA ACCAGTGACC ATGTGCTAGA CCCTTATGAA 
CAATCACTCA GAAACGAAGT GCAGACTGAA CTGCAGCAGC TCGAAGTGAA GATTGAAGAG 
CACTCACAGC TGCTAATCTC AAACAAGAGA CAAATGCAGC TTTATATTAG TCGCCTTCAG 
GAGATCAGAG CATCAATGGA CTTATTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910650.2
CDS	105..3857
Translation	MDFPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIRLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_004910593.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910593.4

ORF Insert Sequence:

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC AGCTGCAACA CAAGAAAAGA TTCAGACGAA 
GAGTCCTTGG CCTTGAAAAT GCCTCTACAG AAAGTCCAAG CAACAAATCC TACCAAACAA 
CTTCCTGGTC TGCCTCTGCA ACACACATGC ACTTCTTGTG TAACAAAGGA GTCCGCTGCT 
GCAATTCAGC TTCCTCCTAC CCTAGGTTCT GCAGCATCAT CAGAACCTCA TCAGTTGATC 
CCAAACCAGG CTTCATCTTG TTCAACACTA ATGCCTCCAT CTTCTTTTGT TCCTCTGGTT 
CAAAGCAAGA TAGACATAGC AAAAAGTGCT CTTCCACACA TTAGAGAACA GCCTCAGATA 
AAGAACCTCT CCAGCACAGA ATTCTCCGAT TTTTCATGTC AGCTACTGTC ACCAACTGAT 
TTCCTTCAAG GCATTGGGCC CCAGGCCCAA AGTGGTACTA CTGCAATTCA AATGGGAAAA 
AGTCCAGAGA AACCAAAAGA GAGCAAACAG ACTGATACCC TTAAAAATGA ACTTCTAGAG 
ATTCAAAGAA TTATGCAGCA ATACCAAGAC CAGAAAGAGG AGCTCAAGGA TTGGCGCAGA 
CATGCAGGAG TGCTACGCAG GTGGCTGAAA ACCAGTGACC ATGTGCTAGA CCCTTATGAA 
CAATCACTCA GAAACGAAGT GCAGACTGAA CTGCAGCAGC TCGAAGTGAA GATTGAAGAG 
CACTCACAGC TGCTAATCTC AAACAAGAGA CAAATGCAGC TTTATATTAG TCGCCTTCAG 
GAGATCAGAG CATCAATGGA CTTATTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_002931922.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931968.3 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X2, mRNA.

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CloneID	OXa48882
Clone ID Related Accession (Same CDS sequence)	XM_002931922.5
Accession Version	XM_002931922.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931922.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATCAAG 
TTGCGAGCAG AGAGAACAAA ACCCCGCAGC CCTGAGTTCC TGCTGCAGTC ACTGGAGAGA 
GAGGGACTAC TGAATGCTGT GGTCAGCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931968.3
CDS	105..3854
Translation	MDFPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIRLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVIKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_002931922.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931922.5

ORF Insert Sequence:

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATCAAG 
TTGCGAGCAG AGAGAACAAA ACCCCGCAGC CCTGAGTTCC TGCTGCAGTC ACTGGAGAGA 
GAGGGACTAC TGAATGCTGT GGTCAGCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_004910594.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910651.2 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa48883
Clone ID Related Accession (Same CDS sequence)	XM_004910594.4
Accession Version	XM_004910594.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910594.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910651.2
CDS	105..3854
Translation	MDFPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIRLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_004910594.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910594.4

ORF Insert Sequence:

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_012969717.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825171.2 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa48884
Clone ID Related Accession (Same CDS sequence)	XM_012969717.3
Accession Version	XM_012969717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969717.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA ATACAGCAAA GTCATTCAAT TCAAAACGAC AGCGTAAAGT TGATCCTCCT 
GTCACATCTG GAAACCAGCG CCCCCAAAAT AGCCGTCTTA TACCATATCG TGTGACATAC 
ACATGGAACC GTGGAGGGAG GCTAAAAGAA TTGCGCATAA GGCATCTGGC CAGAAAATTC 
CTTTACTTGT GGGTGAAAAA AACATTTGGA AGAGTCCTTC CATCTACTGC CAGGCTCCAT 
TATTCTCGCC GCTTGCTACA CTTCTGTTTT CATCGGTGGC AGGAGGAATG GTGGCTCTTG 
TGCATGGAGT GGAAACTTAA TATCAGAGCT GAGTGCCACA ACAGGTATGT CTTATACAAA 
ATGTGTTTCT ATGCCTGGAG GAAATATATT TCATTGCAGC AGAGGAAAAA GTTCAAGGTT 
CAGGCTGCAG AGATCCATGC ACAGACTCAT TTAAAGCATC AGGCACTTCA CCATTGGAAA 
AATTATGTGA AAATACGCAG AACCAAGCAG CGTATGCTGT TAATTGCGCT GGAATTCAGA 
GAACAACAAG ACCTTAGAAA CTCCTGGTAC ATGTGGGTAC TACGGTTAGA GCAGCAACAG 
AGCTACCGTG ATATGGACAT GCTGGCTTTA AATCACTGGG CTGTGAGCCT GCAAAACAGG 
GCTTGGCTGC AGTGGAGAGA GATTTATGTA CATAGAATAT CTGAGAAGAA AAAGGTTTTG 
CAAGCAGTAA AGCATAATCG AAAATGCAAG TTACAAGCGG CGCTGAAAGC CTGGCAGCTA 
TACTTATGTT ATCGTCGAGA GAAACGACGG CATCAAATGA TGGCTGTTCG TTTGCATCAG 
AATTTTCTAA CAAAGAGATA CTTCTTATAC TGGCACCACG CATTGGAGAA AGTTTGGAGT 
ATGCACAGAA TAGAAAAACA TATTGAGGCT TTGGCTGTAC GCTGCCTGCT GCGAAGGACT 
CTAACACATT GGAAAAATTA CATGATGGTA CATTCCGAGA ATATTAGGCT GCAAAAAGTA 
GCAGAAGCGT ATCACAGAAA CAACATAATG AAATTAGGAT TCCACGCCCT GAAAAATAAT 
ATAAACCAAG TTCGCATATA TCAACAACAA AGTCTTCAGG CTGTCCAGCA ATACCAAATT 
ATGCTTATCC ATAAGTACTG GACTGTATGG AAGTTACGCT TGGAACAGAA AGAGGAAGAA 
AAACTTTTGA CATTGACATT AACAGCACAT GCTCATTACA GGTCGGTGTT AATACAGAAG 
TCTTTCAGGG TGTGGTTACA GTACAGACAG AAAAGGAAAC TGAAGCAGGT GCTAAATAAA 
ACAGCGTATA GTCACTATGC AAAATCCTTA TTGCCTCACA GCTTTCAAGT GTGGAGGAAA 
CATCAAGAAC ATCAATGGCA GAAAAGACAC ATGGTAGAAA AAGCAACACA GTTTCACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825171.2
CDS	82..3816
Translation	MEHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIRLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_012969717.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969717.3

ORF Insert Sequence:

ATGGAGCACA ATACAGCAAA GTCATTCAAT TCAAAACGAC AGCGTAAAGT TGATCCTCCT 
GTCACATCTG GAAACCAGCG CCCCCAAAAT AGCCGTCTTA TACCATATCG TGTGACATAC 
ACATGGAACC GTGGAGGGAG GCTAAAAGAA TTGCGCATAA GGCATCTGGC CAGAAAATTC 
CTTTACTTGT GGGTGAAAAA AACATTTGGA AGAGTCCTTC CATCTACTGC CAGGCTCCAT 
TATTCTCGCC GCTTGCTACA CTTCTGTTTT CATCGGTGGC AGGAGGAATG GTGGCTCTTG 
TGCATGGAGT GGAAACTTAA TATCAGAGCT GAGTGCCACA ACAGGTATGT CTTATACAAA 
ATGTGTTTCT ATGCCTGGAG GAAATATATT TCATTGCAGC AGAGGAAAAA GTTCAAGGTT 
CAGGCTGCAG AGATCCATGC ACAGACTCAT TTAAAGCATC AGGCACTTCA CCATTGGAAA 
AATTATGTGA AAATACGCAG AACCAAGCAG CGTATGCTGT TAATTGCGCT GGAATTCAGA 
GAACAACAAG ACCTTAGAAA CTCCTGGTAC ATGTGGGTAC TACGGTTAGA GCAGCAACAG 
AGCTACCGTG ATATGGACAT GCTGGCTTTA AATCACTGGG CTGTGAGCCT GCAAAACAGG 
GCTTGGCTGC AGTGGAGAGA GATTTATGTA CATAGAATAT CTGAGAAGAA AAAGGTTTTG 
CAAGCAGTAA AGCATAATCG AAAATGCAAG TTACAAGCGG CGCTGAAAGC CTGGCAGCTA 
TACTTATGTT ATCGTCGAGA GAAACGACGG CATCAAATGA TGGCTGTTCG TTTGCATCAG 
AATTTTCTAA CAAAGAGATA CTTCTTATAC TGGCACCACG CATTGGAGAA AGTTTGGAGT 
ATGCACAGAA TAGAAAAACA TATTGAGGCT TTGGCTGTAC GCTGCCTGCT GCGAAGGACT 
CTAACACATT GGAAAAATTA CATGATGGTA CATTCCGAGA ATATTAGGCT GCAAAAAGTA 
GCAGAAGCGT ATCACAGAAA CAACATAATG AAATTAGGAT TCCACGCCCT GAAAAATAAT 
ATAAACCAAG TTCGCATATA TCAACAACAA AGTCTTCAGG CTGTCCAGCA ATACCAAATT 
ATGCTTATCC ATAAGTACTG GACTGTATGG AAGTTACGCT TGGAACAGAA AGAGGAAGAA 
AAACTTTTGA CATTGACATT AACAGCACAT GCTCATTACA GGTCGGTGTT AATACAGAAG 
TCTTTCAGGG TGTGGTTACA GTACAGACAG AAAAGGAAAC TGAAGCAGGT GCTAAATAAA 
ACAGCGTATA GTCACTATGC AAAATCCTTA TTGCCTCACA GCTTTCAAGT GTGGAGGAAA 
CATCAAGAAC ATCAATGGCA GAAAAGACAC ATGGTAGAAA AAGCAACACA GTTTCACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_012969720.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825174.2 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X5, mRNA.

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CloneID	OXa48885
Clone ID Related Accession (Same CDS sequence)	XM_012969720.3
Accession Version	XM_012969720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825174.2
CDS	105..3659
Translation	MDFPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIRLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_012969720.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969720.3

ORF Insert Sequence:

ATGGATTTTC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTAGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_018090116.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017945605.2 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X6, mRNA.

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CloneID	OXa48886
Clone ID Related Accession (Same CDS sequence)	XM_018090116.2
Accession Version	XM_018090116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018090116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGA AACTTAATAT CAGAGCTGAG TGCCACAACA GGTATGTCTT ATACAAAATG 
TGTTTCTATG CCTGGAGGAA ATATATTTCA TTGCAGCAGA GGAAAAAGTT CAAGGTTCAG 
GCTGCAGAGA TCCATGCACA GACTCATTTA AAGCATCAGG CACTTCACCA TTGGAAAAAT 
TATGTGAAAA TACGCAGAAC CAAGCAGCGT ATGCTGTTAA TTGCGCTGGA ATTCAGAGAA 
CAACAAGACC TTAGAAACTC CTGGTACATG TGGGTACTAC GGTTAGAGCA GCAACAGAGC 
TACCGTGATA TGGACATGCT GGCTTTAAAT CACTGGGCTG TGAGCCTGCA AAACAGGGCT 
TGGCTGCAGT GGAGAGAGAT TTATGTACAT AGAATATCTG AGAAGAAAAA GGTTTTGCAA 
GCAGTAAAGC ATAATCGAAA ATGCAAGTTA CAAGCGGCGC TGAAAGCCTG GCAGCTATAC 
TTATGTTATC GTCGAGAGAA ACGACGGCAT CAAATGATGG CTGTTCGTTT GCATCAGAAT 
TTTCTAACAA AGAGATACTT CTTATACTGG CACCACGCAT TGGAGAAAGT TTGGAGTATG 
CACAGAATAG AAAAACATAT TGAGGCTTTG GCTGTACGCT GCCTGCTGCG AAGGACTCTA 
ACACATTGGA AAAATTACAT GATGGTACAT TCCGAGAATA TTAGGCTGCA AAAAGTAGCA 
GAAGCGTATC ACAGAAACAA CATAATGAAA TTAGGATTCC ACGCCCTGAA AAATAATATA 
AACCAAGTTC GCATATATCA ACAACAAAGT CTTCAGGCTG TCCAGCAATA CCAAATTATG 
CTTATCCATA AGTACTGGAC TGTATGGAAG TTACGCTTGG AACAGAAAGA GGAAGAAAAA 
CTTTTGACAT TGACATTAAC AGCACATGCT CATTACAGGT CGGTGTTAAT ACAGAAGTCT 
TTCAGGGTGT GGTTACAGTA CAGACAGAAA AGGAAACTGA AGCAGGTGCT AAATAAAACA 
GCGTATAGTC ACTATGCAAA ATCCTTATTG CCTCACAGCT TTCAAGTGTG GAGGAAACAT 
CAAGAACATC AATGGCAGAA AAGACACATG GTAGAAAAAG CAACACAGTT TCACAGGTAT 
ACTGTACAGA GAAGAGTACT CTTCACATGG CATAAGGAGT TAAGCCAACA GAGGGAATAT 
CGCCTGAGTG AGAGAATGGC TATACTACAT TTTAACTGGA GGCTATTAGA ACAATGTTGG 
TACTCCTGGA AAAATCAGTT AGTTCTCCAC ATAGCAGAAA AAAAAGGCAA TTCTGAAGCT 
GCTGAACATC ACAGGAGACA TCAGTTGCTA AATGCCATGA CCATATGGAA GGAACACGTA 
CTGGAGATAA AGGCAGAGAG AGCTCGAGAG GAGAAAGCCA TACTTCACCA CCGTGTGCTT 
TGCATGCAGA ATGCCTGGAA CCACTGGAAA TGGTTTTTGT TTCAGAGACA TGAAAAGTGG 
AAAAAACAAG TCAGGGCTGA TCTACACTAC CAGCGCAACT TGCTTGCAAA TGTATTTCAG 
GCATGGAAAC ATTATCATAT TAGTGTACAG GACATACTGC ATCAGGTGAA GGAGAAAGAG 
AAGCAAAAAA ATGAATCTTT CTTGCGAGGT GCACTGTGTA GCTGGAGGAA TCAAGCATCT 
GCTCAGGTTC TGGAGAGAAG GCAGGAAAGA CTTGCAATTA TTCACTACAG GGCTACAACT 
CAAATAAAGG TTGTTCGGGC ATGGAGAGAT TCTGCAGCTG TGCTGGCACA TTGTAGGCAG 
AAAAAGGCAG AGGAAGTGAA AGAGGCAATG CTTTTTCTAC AGAAAAGAAA GCTTCAGTGT 
TTCTTTATGC ACTGGAGGCA ACTAAGTTAC ACAACAAGGG ACCTTCGTGT TAGAATGGAG 
GCAGCCGCAA CGCATCATGT AAAAAATTTG CTGAAGAATT GCATGACAAA ATGGAAAGCT 
TATCGTGCTC ACTGCTTACG GAAAATGCTA CTCCAGCGTC AAGGAGCCTG GTTTGCAGAA 
CAGAGACTTA GCCGAAATTG TCTTAGACAG TGGTGCCAAA TGCTGGTAGA AAAGCAACAG 
CAGGACAAGA AGACTGTGAA AGCTTTATGG AACTGGTCCC TTACACTGCA GGGAAAGGTG 
TTTGATGGCT GGCTATCTTA TGTCTGTGAG CGTCGGCGAA AAAAGGATCG CATTGTAGCA 
GCGGTAGAAC TGTATCGTGC AGATCTACTG CAAACTGGAG TGACCAGAAT CCTAAATTAT 
GCATCTGGCA TGAAACAATT CAGATCTCAT CTGAACACTC AGCACCAAGT AAAGGAAGTG 
CAGATGCAAA ATGCCGCAGC TCGTCGATGT GCCATAATCT GGAAGGAGAA GGTCTTCAGA 
AGGCTCCATC AAAAAACTTC TCAGAAGAGG GTGACTTTCA GTACTTCGAA AGCTGATTCA 
TTATCTACAG AAGAAGAGAC CTATTCAACA AAGACCTCAA GGGAAATAAT GTGTGCCGTA 
CCCTTGACTA GTGGGGAGCC CACGCTAAGT GTAATTAGCA AGTTGCGAGC AGAGAGAACA 
AAACCCCGCA GCCCTGAGTT CCTGCTGCAG TCACTGGAGA GAGAGGGACT ACTGAATGCT 
GTGGTCAGCA GCTGCAACAC AAGAAAAGAT TCAGACGAAG AGTCCTTGGC CTTGAAAATG 
CCTCTACAGA AAGTCCAAGC AACAAATCCT ACCAAACAAC TTCCTGGTCT GCCTCTGCAA 
CACACATGCA CTTCTTGTGT AACAAAGGAG TCCGCTGCTG CAATTCAGCT TCCTCCTACC 
CTAGGTTCTG CAGCATCATC AGAACCTCAT CAGTTGATCC CAAACCAGGC TTCATCTTGT 
TCAACACTAA TGCCTCCATC TTCTTTTGTT CCTCTGGTTC AAAGCAAGAT AGACATAGCA 
AAAAGTGCTC TTCCACACAT TAGAGAACAG CCTCAGATAA AGAACCTCTC CAGCACAGAA 
TTCTCCGATT TTTCATGTCA GCTACTGTCA CCAACTGATT TCCTTCAAGG CATTGGGCCC 
CAGGCCCAAA GTGGTACTAC TGCAATTCAA ATGGGAAAAA GTCCAGAGAA ACCAAAAGAG 
AGCAAACAGA CTGATACCCT TAAAAATGAA CTTCTAGAGA TTCAAAGAAT TATGCAGCAA 
TACCAAGACC AGAAAGAGGA GCTCAAGGAT TGGCGCAGAC ATGCAGGAGT GCTACGCAGG 
TGGCTGAAAA CCAGTGACCA TGTGCTAGAC CCTTATGAAC AATCACTCAG AAACGAAGTG 
CAGACTGAAC TGCAGCAGCT CGAAGTGAAG ATTGAAGAGC ACTCACAGCT GCTAATCTCA 
AACAAGAGAC AAATGCAGCT TTATATTAGT CGCCTTCAGG AGATCAGAGC ATCAATGGAC 
TTATTGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017945605.2
CDS	446..3877
Translation	MEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIRLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_018090116.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090116.2

ORF Insert Sequence:

ATGGAGTGGA AACTTAATAT CAGAGCTGAG TGCCACAACA GGTATGTCTT ATACAAAATG 
TGTTTCTATG CCTGGAGGAA ATATATTTCA TTGCAGCAGA GGAAAAAGTT CAAGGTTCAG 
GCTGCAGAGA TCCATGCACA GACTCATTTA AAGCATCAGG CACTTCACCA TTGGAAAAAT 
TATGTGAAAA TACGCAGAAC CAAGCAGCGT ATGCTGTTAA TTGCGCTGGA ATTCAGAGAA 
CAACAAGACC TTAGAAACTC CTGGTACATG TGGGTACTAC GGTTAGAGCA GCAACAGAGC 
TACCGTGATA TGGACATGCT GGCTTTAAAT CACTGGGCTG TGAGCCTGCA AAACAGGGCT 
TGGCTGCAGT GGAGAGAGAT TTATGTACAT AGAATATCTG AGAAGAAAAA GGTTTTGCAA 
GCAGTAAAGC ATAATCGAAA ATGCAAGTTA CAAGCGGCGC TGAAAGCCTG GCAGCTATAC 
TTATGTTATC GTCGAGAGAA ACGACGGCAT CAAATGATGG CTGTTCGTTT GCATCAGAAT 
TTTCTAACAA AGAGATACTT CTTATACTGG CACCACGCAT TGGAGAAAGT TTGGAGTATG 
CACAGAATAG AAAAACATAT TGAGGCTTTG GCTGTACGCT GCCTGCTGCG AAGGACTCTA 
ACACATTGGA AAAATTACAT GATGGTACAT TCCGAGAATA TTAGGCTGCA AAAAGTAGCA 
GAAGCGTATC ACAGAAACAA CATAATGAAA TTAGGATTCC ACGCCCTGAA AAATAATATA 
AACCAAGTTC GCATATATCA ACAACAAAGT CTTCAGGCTG TCCAGCAATA CCAAATTATG 
CTTATCCATA AGTACTGGAC TGTATGGAAG TTACGCTTGG AACAGAAAGA GGAAGAAAAA 
CTTTTGACAT TGACATTAAC AGCACATGCT CATTACAGGT CGGTGTTAAT ACAGAAGTCT 
TTCAGGGTGT GGTTACAGTA CAGACAGAAA AGGAAACTGA AGCAGGTGCT AAATAAAACA 
GCGTATAGTC ACTATGCAAA ATCCTTATTG CCTCACAGCT TTCAAGTGTG GAGGAAACAT 
CAAGAACATC AATGGCAGAA AAGACACATG GTAGAAAAAG CAACACAGTT TCACAGGTAT 
ACTGTACAGA GAAGAGTACT CTTCACATGG CATAAGGAGT TAAGCCAACA GAGGGAATAT 
CGCCTGAGTG AGAGAATGGC TATACTACAT TTTAACTGGA GGCTATTAGA ACAATGTTGG 
TACTCCTGGA AAAATCAGTT AGTTCTCCAC ATAGCAGAAA AAAAAGGCAA TTCTGAAGCT 
GCTGAACATC ACAGGAGACA TCAGTTGCTA AATGCCATGA CCATATGGAA GGAACACGTA 
CTGGAGATAA AGGCAGAGAG AGCTCGAGAG GAGAAAGCCA TACTTCACCA CCGTGTGCTT 
TGCATGCAGA ATGCCTGGAA CCACTGGAAA TGGTTTTTGT TTCAGAGACA TGAAAAGTGG 
AAAAAACAAG TCAGGGCTGA TCTACACTAC CAGCGCAACT TGCTTGCAAA TGTATTTCAG 
GCATGGAAAC ATTATCATAT TAGTGTACAG GACATACTGC ATCAGGTGAA GGAGAAAGAG 
AAGCAAAAAA ATGAATCTTT CTTGCGAGGT GCACTGTGTA GCTGGAGGAA TCAAGCATCT 
GCTCAGGTTC TGGAGAGAAG GCAGGAAAGA CTTGCAATTA TTCACTACAG GGCTACAACT 
CAAATAAAGG TTGTTCGGGC ATGGAGAGAT TCTGCAGCTG TGCTGGCACA TTGTAGGCAG 
AAAAAGGCAG AGGAAGTGAA AGAGGCAATG CTTTTTCTAC AGAAAAGAAA GCTTCAGTGT 
TTCTTTATGC ACTGGAGGCA ACTAAGTTAC ACAACAAGGG ACCTTCGTGT TAGAATGGAG 
GCAGCCGCAA CGCATCATGT AAAAAATTTG CTGAAGAATT GCATGACAAA ATGGAAAGCT 
TATCGTGCTC ACTGCTTACG GAAAATGCTA CTCCAGCGTC AAGGAGCCTG GTTTGCAGAA 
CAGAGACTTA GCCGAAATTG TCTTAGACAG TGGTGCCAAA TGCTGGTAGA AAAGCAACAG 
CAGGACAAGA AGACTGTGAA AGCTTTATGG AACTGGTCCC TTACACTGCA GGGAAAGGTG 
TTTGATGGCT GGCTATCTTA TGTCTGTGAG CGTCGGCGAA AAAAGGATCG CATTGTAGCA 
GCGGTAGAAC TGTATCGTGC AGATCTACTG CAAACTGGAG TGACCAGAAT CCTAAATTAT 
GCATCTGGCA TGAAACAATT CAGATCTCAT CTGAACACTC AGCACCAAGT AAAGGAAGTG 
CAGATGCAAA ATGCCGCAGC TCGTCGATGT GCCATAATCT GGAAGGAGAA GGTCTTCAGA 
AGGCTCCATC AAAAAACTTC TCAGAAGAGG GTGACTTTCA GTACTTCGAA AGCTGATTCA 
TTATCTACAG AAGAAGAGAC CTATTCAACA AAGACCTCAA GGGAAATAAT GTGTGCCGTA 
CCCTTGACTA GTGGGGAGCC CACGCTAAGT GTAATTAGCA AGTTGCGAGC AGAGAGAACA 
AAACCCCGCA GCCCTGAGTT CCTGCTGCAG TCACTGGAGA GAGAGGGACT ACTGAATGCT 
GTGGTCAGCA GCTGCAACAC AAGAAAAGAT TCAGACGAAG AGTCCTTGGC CTTGAAAATG 
CCTCTACAGA AAGTCCAAGC AACAAATCCT ACCAAACAAC TTCCTGGTCT GCCTCTGCAA 
CACACATGCA CTTCTTGTGT AACAAAGGAG TCCGCTGCTG CAATTCAGCT TCCTCCTACC 
CTAGGTTCTG CAGCATCATC AGAACCTCAT CAGTTGATCC CAAACCAGGC TTCATCTTGT 
TCAACACTAA TGCCTCCATC TTCTTTTGTT CCTCTGGTTC AAAGCAAGAT AGACATAGCA 
AAAAGTGCTC TTCCACACAT TAGAGAACAG CCTCAGATAA AGAACCTCTC CAGCACAGAA 
TTCTCCGATT TTTCATGTCA GCTACTGTCA CCAACTGATT TCCTTCAAGG CATTGGGCCC 
CAGGCCCAAA GTGGTACTAC TGCAATTCAA ATGGGAAAAA GTCCAGAGAA ACCAAAAGAG 
AGCAAACAGA CTGATACCCT TAAAAATGAA CTTCTAGAGA TTCAAAGAAT TATGCAGCAA 
TACCAAGACC AGAAAGAGGA GCTCAAGGAT TGGCGCAGAC ATGCAGGAGT GCTACGCAGG 
TGGCTGAAAA CCAGTGACCA TGTGCTAGAC CCTTATGAAC AATCACTCAG AAACGAAGTG 
CAGACTGAAC TGCAGCAGCT CGAAGTGAAG ATTGAAGAGC ACTCACAGCT GCTAATCTCA 
AACAAGAGAC AAATGCAGCT TTATATTAGT CGCCTTCAGG AGATCAGAGC ATCAATGGAC 
TTATTGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_004910593.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910650.1 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X1, mRNA.

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CloneID	OXa03646
Clone ID Related Accession (Same CDS sequence)	XM_004910593.3
Accession Version	XM_004910593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC AGCTGCAACA CAAGAAAAGA TTCAGACGAA 
GAGTCCTTGG CCTTGAAAAT GCCTCTACAG AAAGTCCAAG CAACAAATCC TACCAAACAA 
CTTCCTGGTC TGCCTCTGCA ACACACATGC ACTTCTTGTG TAACAAAGGA GTCCGCTGCT 
GCAATTCAGC TTCCTCCTAC CCTAGGTTCT GCAGCATCAT CAGAACCTCA TCAGTTGATC 
CCAAACCAGG CTTCATCTTG TTCAACACTA ATGCCTCCAT CTTCTTTTGT TCCTCTGGTT 
CAAAGCAAGA TAGACATAGC AAAAAGTGCT CTTCCACACA TTAGAGAACA GCCTCAGATA 
AAGAACCTCT CCAGCACAGA ATTCTCCGAT TTTTCATGTC AGCTACTGTC ACCAACTGAT 
TTCCTTCAAG GCATTGGGCC CCAGGCCCAA AGTGGTACTA CTGCAATTCA AATGGGAAAA 
AGTCCAGAGA AACCAAAAGA GAGCAAACAG ACTGATACCC TTAAAAATGA ACTTCTAGAG 
ATTCAAAGAA TTATGCAGCA ATACCAAGAC CAGAAAGAGG AGCTCAAGGA TTGGCGCAGA 
CATGCAGGAG TGCTACGCAG GTGGCTGAAA ACCAGTGACC ATGTGCTAGA CCCTTATGAA 
CAATCACTCA GAAACGAAGT GCAGACTGAA CTGCAGCAGC TCGAAGTGAA GATTGAAGAG 
CACTCACAGC TGCTAATCTC AAACAAGAGA CAAATGCAGC TTTATATTAG TCGCCTTCAG 
GAGATCAGAG CATCAATGGA CTTATTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910650.1
CDS	105..3857
Translation	MDLPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIGLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_004910593.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910593.3

ORF Insert Sequence:

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC AGCTGCAACA CAAGAAAAGA TTCAGACGAA 
GAGTCCTTGG CCTTGAAAAT GCCTCTACAG AAAGTCCAAG CAACAAATCC TACCAAACAA 
CTTCCTGGTC TGCCTCTGCA ACACACATGC ACTTCTTGTG TAACAAAGGA GTCCGCTGCT 
GCAATTCAGC TTCCTCCTAC CCTAGGTTCT GCAGCATCAT CAGAACCTCA TCAGTTGATC 
CCAAACCAGG CTTCATCTTG TTCAACACTA ATGCCTCCAT CTTCTTTTGT TCCTCTGGTT 
CAAAGCAAGA TAGACATAGC AAAAAGTGCT CTTCCACACA TTAGAGAACA GCCTCAGATA 
AAGAACCTCT CCAGCACAGA ATTCTCCGAT TTTTCATGTC AGCTACTGTC ACCAACTGAT 
TTCCTTCAAG GCATTGGGCC CCAGGCCCAA AGTGGTACTA CTGCAATTCA AATGGGAAAA 
AGTCCAGAGA AACCAAAAGA GAGCAAACAG ACTGATACCC TTAAAAATGA ACTTCTAGAG 
ATTCAAAGAA TTATGCAGCA ATACCAAGAC CAGAAAGAGG AGCTCAAGGA TTGGCGCAGA 
CATGCAGGAG TGCTACGCAG GTGGCTGAAA ACCAGTGACC ATGTGCTAGA CCCTTATGAA 
CAATCACTCA GAAACGAAGT GCAGACTGAA CTGCAGCAGC TCGAAGTGAA GATTGAAGAG 
CACTCACAGC TGCTAATCTC AAACAAGAGA CAAATGCAGC TTTATATTAG TCGCCTTCAG 
GAGATCAGAG CATCAATGGA CTTATTGTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_002931922.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931968.2 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03647
Clone ID Related Accession (Same CDS sequence)	XM_002931922.4
Accession Version	XM_002931922.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931922.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATCAAG 
TTGCGAGCAG AGAGAACAAA ACCCCGCAGC CCTGAGTTCC TGCTGCAGTC ACTGGAGAGA 
GAGGGACTAC TGAATGCTGT GGTCAGCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931968.2
CDS	105..3854
Translation	MDLPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIGLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVIKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_002931922.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931922.4

ORF Insert Sequence:

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATCAAG 
TTGCGAGCAG AGAGAACAAA ACCCCGCAGC CCTGAGTTCC TGCTGCAGTC ACTGGAGAGA 
GAGGGACTAC TGAATGCTGT GGTCAGCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_004910594.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910651.1 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa03648
Clone ID Related Accession (Same CDS sequence)	XM_004910594.3
Accession Version	XM_004910594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910651.1
CDS	105..3854
Translation	MDLPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIGLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_004910594.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910594.3

ORF Insert Sequence:

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TCGGTGTTAA TACAGAAGTC TTTCAGGGTG TGGTTACAGT ACAGACAGAA AAGGAAACTG 
AAGCAGGTGC TAAATAAAAC AGCGTATAGT CACTATGCAA AATCCTTATT GCCTCACAGC 
TTTCAAGTGT GGAGGAAACA TCAAGAACAT CAATGGCAGA AAAGACACAT GGTAGAAAAA 
GCAACACAGT TTCACAGGTA TACTGTACAG AGAAGAGTAC TCTTCACATG GCATAAGGAG 
TTAAGCCAAC AGAGGGAATA TCGCCTGAGT GAGAGAATGG CTATACTACA TTTTAACTGG 
AGGCTATTAG AACAATGTTG GTACTCCTGG AAAAATCAGT TAGTTCTCCA CATAGCAGAA 
AAAAAAGGCA ATTCTGAAGC TGCTGAACAT CACAGGAGAC ATCAGTTGCT AAATGCCATG 
ACCATATGGA AGGAACACGT ACTGGAGATA AAGGCAGAGA GAGCTCGAGA GGAGAAAGCC 
ATACTTCACC ACCGTGTGCT TTGCATGCAG AATGCCTGGA ACCACTGGAA ATGGTTTTTG 
TTTCAGAGAC ATGAAAAGTG GAAAAAACAA GTCAGGGCTG ATCTACACTA CCAGCGCAAC 
TTGCTTGCAA ATGTATTTCA GGCATGGAAA CATTATCATA TTAGTGTACA GGACATACTG 
CATCAGGTGA AGGAGAAAGA GAAGCAAAAA AATGAATCTT TCTTGCGAGG TGCACTGTGT 
AGCTGGAGGA ATCAAGCATC TGCTCAGGTT CTGGAGAGAA GGCAGGAAAG ACTTGCAATT 
ATTCACTACA GGGCTACAAC TCAAATAAAG GTTGTTCGGG CATGGAGAGA TTCTGCAGCT 
GTGCTGGCAC ATTGTAGGCA GAAAAAGGCA GAGGAAGTGA AAGAGGCAAT GCTTTTTCTA 
CAGAAAAGAA AGCTTCAGTG TTTCTTTATG CACTGGAGGC AACTAAGTTA CACAACAAGG 
GACCTTCGTG TTAGAATGGA GGCAGCCGCA ACGCATCATG TAAAAAATTT GCTGAAGAAT 
TGCATGACAA AATGGAAAGC TTATCGTGCT CACTGCTTAC GGAAAATGCT ACTCCAGCGT 
CAAGGAGCCT GGTTTGCAGA ACAGAGACTT AGCCGAAATT GTCTTAGACA GTGGTGCCAA 
ATGCTGGTAG AAAAGCAACA GCAGGACAAG AAGACTGTGA AAGCTTTATG GAACTGGTCC 
CTTACACTGC AGGGAAAGGT GTTTGATGGC TGGCTATCTT ATGTCTGTGA GCGTCGGCGA 
AAAAAGGATC GCATTGTAGC AGCGGTAGAA CTGTATCGTG CAGATCTACT GCAAACTGGA 
GTGACCAGAA TCCTAAATTA TGCATCTGGC ATGAAACAAT TCAGATCTCA TCTGAACACT 
CAGCACCAAG TAAAGGAAGT GCAGATGCAA AATGCCGCAG CTCGTCGATG TGCCATAATC 
TGGAAGGAGA AGGTCTTCAG AAGGCTCCAT CAAAAAACTT CTCAGAAGAG GGTGACTTTC 
AGTACTTCGA AAGCTGATTC ATTATCTACA GAAGAAGAGA CCTATTCAAC AAAGACCTCA 
AGGGAAATAA TGTGTGCCGT ACCCTTGACT AGTGGGGAGC CCACGCTAAG TGTAATTAGC 
AAGTTGCGAG CAGAGAGAAC AAAACCCCGC AGCCCTGAGT TCCTGCTGCA GTCACTGGAG 
AGAGAGGGAC TACTGAATGC TGTGGTCAGC TGCAACACAA GAAAAGATTC AGACGAAGAG 
TCCTTGGCCT TGAAAATGCC TCTACAGAAA GTCCAAGCAA CAAATCCTAC CAAACAACTT 
CCTGGTCTGC CTCTGCAACA CACATGCACT TCTTGTGTAA CAAAGGAGTC CGCTGCTGCA 
ATTCAGCTTC CTCCTACCCT AGGTTCTGCA GCATCATCAG AACCTCATCA GTTGATCCCA 
AACCAGGCTT CATCTTGTTC AACACTAATG CCTCCATCTT CTTTTGTTCC TCTGGTTCAA 
AGCAAGATAG ACATAGCAAA AAGTGCTCTT CCACACATTA GAGAACAGCC TCAGATAAAG 
AACCTCTCCA GCACAGAATT CTCCGATTTT TCATGTCAGC TACTGTCACC AACTGATTTC 
CTTCAAGGCA TTGGGCCCCA GGCCCAAAGT GGTACTACTG CAATTCAAAT GGGAAAAAGT 
CCAGAGAAAC CAAAAGAGAG CAAACAGACT GATACCCTTA AAAATGAACT TCTAGAGATT 
CAAAGAATTA TGCAGCAATA CCAAGACCAG AAAGAGGAGC TCAAGGATTG GCGCAGACAT 
GCAGGAGTGC TACGCAGGTG GCTGAAAACC AGTGACCATG TGCTAGACCC TTATGAACAA 
TCACTCAGAA ACGAAGTGCA GACTGAACTG CAGCAGCTCG AAGTGAAGAT TGAAGAGCAC 
TCACAGCTGC TAATCTCAAA CAAGAGACAA ATGCAGCTTT ATATTAGTCG CCTTCAGGAG 
ATCAGAGCAT CAATGGACTT ATTGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_012969717.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825171.1 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X4, mRNA.

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CloneID	OXa33152
Clone ID Related Accession (Same CDS sequence)	XM_012969717.2
Accession Version	XM_012969717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA ATACAGCAAA GTCATTCAAT TCAAAACGAC AGCGTAAAGT TGATCCTCCT 
GTCACATCTG GAAACCAGCG CCCCCAAAAT AGCCGTCTTA TACCATATCG TGTGACATAC 
ACATGGAACC GTGGAGGGAG GCTAAAAGAA TTGCGCATAA GGCATCTGGC CAGAAAATTC 
CTTTACTTGT GGGTGAAAAA AACATTTGGA AGAGTCCTTC CATCTACTGC CAGGCTCCAT 
TATTCTCGCC GCTTGCTACA CTTCTGTTTT CATCGGTGGC AGGAGGAATG GTGGCTCTTG 
TGCATGGAGT GGAAACTTAA TATCAGAGCT GAGTGCCACA ACAGGTATGT CTTATACAAA 
ATGTGTTTCT ATGCCTGGAG GAAATATATT TCATTGCAGC AGAGGAAAAA GTTCAAGGTT 
CAGGCTGCAG AGATCCATGC ACAGACTCAT TTAAAGCATC AGGCACTTCA CCATTGGAAA 
AATTATGTGA AAATACGCAG AACCAAGCAG CGTATGCTGT TAATTGCGCT GGAATTCAGA 
GAACAACAAG ACCTTAGAAA CTCCTGGTAC ATGTGGGTAC TACGGTTAGA GCAGCAACAG 
AGCTACCGTG ATATGGACAT GCTGGCTTTA AATCACTGGG CTGTGAGCCT GCAAAACAGG 
GCTTGGCTGC AGTGGAGAGA GATTTATGTA CATAGAATAT CTGAGAAGAA AAAGGTTTTG 
CAAGCAGTAA AGCATAATCG AAAATGCAAG TTACAAGCGG CGCTGAAAGC CTGGCAGCTA 
TACTTATGTT ATCGTCGAGA GAAACGACGG CATCAAATGA TGGCTGTTCG TTTGCATCAG 
AATTTTCTAA CAAAGAGATA CTTCTTATAC TGGCACCACG CATTGGAGAA AGTTTGGAGT 
ATGCACAGAA TAGAAAAACA TATTGAGGCT TTGGCTGTAC GCTGCCTGCT GCGAAGGACT 
CTAACACATT GGAAAAATTA CATGATGGTA CATTCCGAGA ATATTGGGCT GCAAAAAGTA 
GCAGAAGCGT ATCACAGAAA CAACATAATG AAATTAGGAT TCCACGCCCT GAAAAATAAT 
ATAAACCAAG TTCGCATATA TCAACAACAA AGTCTTCAGG CTGTCCAGCA ATACCAAATT 
ATGCTTATCC ATAAGTACTG GACTGTATGG AAGTTACGCT TGGAACAGAA AGAGGAAGAA 
AAACTTTTGA CATTGACATT AACAGCACAT GCTCATTACA GGTCGGTGTT AATACAGAAG 
TCTTTCAGGG TGTGGTTACA GTACAGACAG AAAAGGAAAC TGAAGCAGGT GCTAAATAAA 
ACAGCGTATA GTCACTATGC AAAATCCTTA TTGCCTCACA GCTTTCAAGT GTGGAGGAAA 
CATCAAGAAC ATCAATGGCA GAAAAGACAC ATGGTAGAAA AAGCAACACA GTTTCACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825171.1
CDS	81..3815
Translation	MEHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIGLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRSVLIQKSFRVWLQYRQKRKLKQVLNKTAYSHYAKSLLPHSFQVWRKHQEHQWQKRHMVEKATQFHRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_012969717.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969717.2

ORF Insert Sequence:

ATGGAGCACA ATACAGCAAA GTCATTCAAT TCAAAACGAC AGCGTAAAGT TGATCCTCCT 
GTCACATCTG GAAACCAGCG CCCCCAAAAT AGCCGTCTTA TACCATATCG TGTGACATAC 
ACATGGAACC GTGGAGGGAG GCTAAAAGAA TTGCGCATAA GGCATCTGGC CAGAAAATTC 
CTTTACTTGT GGGTGAAAAA AACATTTGGA AGAGTCCTTC CATCTACTGC CAGGCTCCAT 
TATTCTCGCC GCTTGCTACA CTTCTGTTTT CATCGGTGGC AGGAGGAATG GTGGCTCTTG 
TGCATGGAGT GGAAACTTAA TATCAGAGCT GAGTGCCACA ACAGGTATGT CTTATACAAA 
ATGTGTTTCT ATGCCTGGAG GAAATATATT TCATTGCAGC AGAGGAAAAA GTTCAAGGTT 
CAGGCTGCAG AGATCCATGC ACAGACTCAT TTAAAGCATC AGGCACTTCA CCATTGGAAA 
AATTATGTGA AAATACGCAG AACCAAGCAG CGTATGCTGT TAATTGCGCT GGAATTCAGA 
GAACAACAAG ACCTTAGAAA CTCCTGGTAC ATGTGGGTAC TACGGTTAGA GCAGCAACAG 
AGCTACCGTG ATATGGACAT GCTGGCTTTA AATCACTGGG CTGTGAGCCT GCAAAACAGG 
GCTTGGCTGC AGTGGAGAGA GATTTATGTA CATAGAATAT CTGAGAAGAA AAAGGTTTTG 
CAAGCAGTAA AGCATAATCG AAAATGCAAG TTACAAGCGG CGCTGAAAGC CTGGCAGCTA 
TACTTATGTT ATCGTCGAGA GAAACGACGG CATCAAATGA TGGCTGTTCG TTTGCATCAG 
AATTTTCTAA CAAAGAGATA CTTCTTATAC TGGCACCACG CATTGGAGAA AGTTTGGAGT 
ATGCACAGAA TAGAAAAACA TATTGAGGCT TTGGCTGTAC GCTGCCTGCT GCGAAGGACT 
CTAACACATT GGAAAAATTA CATGATGGTA CATTCCGAGA ATATTGGGCT GCAAAAAGTA 
GCAGAAGCGT ATCACAGAAA CAACATAATG AAATTAGGAT TCCACGCCCT GAAAAATAAT 
ATAAACCAAG TTCGCATATA TCAACAACAA AGTCTTCAGG CTGTCCAGCA ATACCAAATT 
ATGCTTATCC ATAAGTACTG GACTGTATGG AAGTTACGCT TGGAACAGAA AGAGGAAGAA 
AAACTTTTGA CATTGACATT AACAGCACAT GCTCATTACA GGTCGGTGTT AATACAGAAG 
TCTTTCAGGG TGTGGTTACA GTACAGACAG AAAAGGAAAC TGAAGCAGGT GCTAAATAAA 
ACAGCGTATA GTCACTATGC AAAATCCTTA TTGCCTCACA GCTTTCAAGT GTGGAGGAAA 
CATCAAGAAC ATCAATGGCA GAAAAGACAC ATGGTAGAAA AAGCAACACA GTTTCACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfi1 ( XM_012969720.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825174.1 Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X5, mRNA.

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CloneID	OXa33153
Clone ID Related Accession (Same CDS sequence)	XM_012969720.2
Accession Version	XM_012969720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein SFI1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825174.1
CDS	105..3659
Translation	MDLPVTVRHNTAKSFNSKRQRKVDPPVTSGNQRPQNSRLIPYRVTYTWNRGGRLKELRIRHLARKFLYLWVKKTFGRVLPSTARLHYSRRLLHFCFHRWQEEWWLLCMEWKLNIRAECHNRYVLYKMCFYAWRKYISLQQRKKFKVQAAEIHAQTHLKHQALHHWKNYVKIRRTKQRMLLIALEFREQQDLRNSWYMWVLRLEQQQSYRDMDMLALNHWAVSLQNRAWLQWREIYVHRISEKKKVLQAVKHNRKCKLQAALKAWQLYLCYRREKRRHQMMAVRLHQNFLTKRYFLYWHHALEKVWSMHRIEKHIEALAVRCLLRRTLTHWKNYMMVHSENIGLQKVAEAYHRNNIMKLGFHALKNNINQVRIYQQQSLQAVQQYQIMLIHKYWTVWKLRLEQKEEEKLLTLTLTAHAHYRYTVQRRVLFTWHKELSQQREYRLSERMAILHFNWRLLEQCWYSWKNQLVLHIAEKKGNSEAAEHHRRHQLLNAMTIWKEHVLEIKAERAREEKAILHHRVLCMQNAWNHWKWFLFQRHEKWKKQVRADLHYQRNLLANVFQAWKHYHISVQDILHQVKEKEKQKNESFLRGALCSWRNQASAQVLERRQERLAIIHYRATTQIKVVRAWRDSAAVLAHCRQKKAEEVKEAMLFLQKRKLQCFFMHWRQLSYTTRDLRVRMEAAATHHVKNLLKNCMTKWKAYRAHCLRKMLLQRQGAWFAEQRLSRNCLRQWCQMLVEKQQQDKKTVKALWNWSLTLQGKVFDGWLSYVCERRRKKDRIVAAVELYRADLLQTGVTRILNYASGMKQFRSHLNTQHQVKEVQMQNAAARRCAIIWKEKVFRRLHQKTSQKRVTFSTSKADSLSTEEETYSTKTSREIMCAVPLTSGEPTLSVISKLRAERTKPRSPEFLLQSLEREGLLNAVVSSCNTRKDSDEESLALKMPLQKVQATNPTKQLPGLPLQHTCTSCVTKESAAAIQLPPTLGSAASSEPHQLIPNQASSCSTLMPPSSFVPLVQSKIDIAKSALPHIREQPQIKNLSSTEFSDFSCQLLSPTDFLQGIGPQAQSGTTAIQMGKSPEKPKESKQTDTLKNELLEIQRIMQQYQDQKEELKDWRRHAGVLRRWLKTSDHVLDPYEQSLRNEVQTELQQLEVKIEEHSQLLISNKRQMQLYISRLQEIRASMDLLL

Target ORF information:

RefSeq Version	XM_012969720.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SFI1 centrin binding protein (sfi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969720.2

ORF Insert Sequence:

ATGGATTTGC CAGTCACAGT CCGGCACAAT ACAGCAAAGT CATTCAATTC AAAACGACAG 
CGTAAAGTTG ATCCTCCTGT CACATCTGGA AACCAGCGCC CCCAAAATAG CCGTCTTATA 
CCATATCGTG TGACATACAC ATGGAACCGT GGAGGGAGGC TAAAAGAATT GCGCATAAGG 
CATCTGGCCA GAAAATTCCT TTACTTGTGG GTGAAAAAAA CATTTGGAAG AGTCCTTCCA 
TCTACTGCCA GGCTCCATTA TTCTCGCCGC TTGCTACACT TCTGTTTTCA TCGGTGGCAG 
GAGGAATGGT GGCTCTTGTG CATGGAGTGG AAACTTAATA TCAGAGCTGA GTGCCACAAC 
AGGTATGTCT TATACAAAAT GTGTTTCTAT GCCTGGAGGA AATATATTTC ATTGCAGCAG 
AGGAAAAAGT TCAAGGTTCA GGCTGCAGAG ATCCATGCAC AGACTCATTT AAAGCATCAG 
GCACTTCACC ATTGGAAAAA TTATGTGAAA ATACGCAGAA CCAAGCAGCG TATGCTGTTA 
ATTGCGCTGG AATTCAGAGA ACAACAAGAC CTTAGAAACT CCTGGTACAT GTGGGTACTA 
CGGTTAGAGC AGCAACAGAG CTACCGTGAT ATGGACATGC TGGCTTTAAA TCACTGGGCT 
GTGAGCCTGC AAAACAGGGC TTGGCTGCAG TGGAGAGAGA TTTATGTACA TAGAATATCT 
GAGAAGAAAA AGGTTTTGCA AGCAGTAAAG CATAATCGAA AATGCAAGTT ACAAGCGGCG 
CTGAAAGCCT GGCAGCTATA CTTATGTTAT CGTCGAGAGA AACGACGGCA TCAAATGATG 
GCTGTTCGTT TGCATCAGAA TTTTCTAACA AAGAGATACT TCTTATACTG GCACCACGCA 
TTGGAGAAAG TTTGGAGTAT GCACAGAATA GAAAAACATA TTGAGGCTTT GGCTGTACGC 
TGCCTGCTGC GAAGGACTCT AACACATTGG AAAAATTACA TGATGGTACA TTCCGAGAAT 
ATTGGGCTGC AAAAAGTAGC AGAAGCGTAT CACAGAAACA ACATAATGAA ATTAGGATTC 
CACGCCCTGA AAAATAATAT AAACCAAGTT CGCATATATC AACAACAAAG TCTTCAGGCT 
GTCCAGCAAT ACCAAATTAT GCTTATCCAT AAGTACTGGA CTGTATGGAA GTTACGCTTG 
GAACAGAAAG AGGAAGAAAA ACTTTTGACA TTGACATTAA CAGCACATGC TCATTACAGG 
TATACTGTAC AGAGAAGAGT ACTCTTCACA TGGCATAAGG AGTTAAGCCA ACAGAGGGAA 
TATCGCCTGA GTGAGAGAAT GGCTATACTA CATTTTAACT GGAGGCTATT AGAACAATGT 
TGGTACTCCT GGAAAAATCA GTTAGTTCTC CACATAGCAG AAAAAAAAGG CAATTCTGAA 
GCTGCTGAAC ATCACAGGAG ACATCAGTTG CTAAATGCCA TGACCATATG GAAGGAACAC 
GTACTGGAGA TAAAGGCAGA GAGAGCTCGA GAGGAGAAAG CCATACTTCA CCACCGTGTG 
CTTTGCATGC AGAATGCCTG GAACCACTGG AAATGGTTTT TGTTTCAGAG ACATGAAAAG 
TGGAAAAAAC AAGTCAGGGC TGATCTACAC TACCAGCGCA ACTTGCTTGC AAATGTATTT 
CAGGCATGGA AACATTATCA TATTAGTGTA CAGGACATAC TGCATCAGGT GAAGGAGAAA 
GAGAAGCAAA AAAATGAATC TTTCTTGCGA GGTGCACTGT GTAGCTGGAG GAATCAAGCA 
TCTGCTCAGG TTCTGGAGAG AAGGCAGGAA AGACTTGCAA TTATTCACTA CAGGGCTACA 
ACTCAAATAA AGGTTGTTCG GGCATGGAGA GATTCTGCAG CTGTGCTGGC ACATTGTAGG 
CAGAAAAAGG CAGAGGAAGT GAAAGAGGCA ATGCTTTTTC TACAGAAAAG AAAGCTTCAG 
TGTTTCTTTA TGCACTGGAG GCAACTAAGT TACACAACAA GGGACCTTCG TGTTAGAATG 
GAGGCAGCCG CAACGCATCA TGTAAAAAAT TTGCTGAAGA ATTGCATGAC AAAATGGAAA 
GCTTATCGTG CTCACTGCTT ACGGAAAATG CTACTCCAGC GTCAAGGAGC CTGGTTTGCA 
GAACAGAGAC TTAGCCGAAA TTGTCTTAGA CAGTGGTGCC AAATGCTGGT AGAAAAGCAA 
CAGCAGGACA AGAAGACTGT GAAAGCTTTA TGGAACTGGT CCCTTACACT GCAGGGAAAG 
GTGTTTGATG GCTGGCTATC TTATGTCTGT GAGCGTCGGC GAAAAAAGGA TCGCATTGTA 
GCAGCGGTAG AACTGTATCG TGCAGATCTA CTGCAAACTG GAGTGACCAG AATCCTAAAT 
TATGCATCTG GCATGAAACA ATTCAGATCT CATCTGAACA CTCAGCACCA AGTAAAGGAA 
GTGCAGATGC AAAATGCCGC AGCTCGTCGA TGTGCCATAA TCTGGAAGGA GAAGGTCTTC 
AGAAGGCTCC ATCAAAAAAC TTCTCAGAAG AGGGTGACTT TCAGTACTTC GAAAGCTGAT 
TCATTATCTA CAGAAGAAGA GACCTATTCA ACAAAGACCT CAAGGGAAAT AATGTGTGCC 
GTACCCTTGA CTAGTGGGGA GCCCACGCTA AGTGTAATTA GCAAGTTGCG AGCAGAGAGA 
ACAAAACCCC GCAGCCCTGA GTTCCTGCTG CAGTCACTGG AGAGAGAGGG ACTACTGAAT 
GCTGTGGTCA GCAGCTGCAA CACAAGAAAA GATTCAGACG AAGAGTCCTT GGCCTTGAAA 
ATGCCTCTAC AGAAAGTCCA AGCAACAAAT CCTACCAAAC AACTTCCTGG TCTGCCTCTG 
CAACACACAT GCACTTCTTG TGTAACAAAG GAGTCCGCTG CTGCAATTCA GCTTCCTCCT 
ACCCTAGGTT CTGCAGCATC ATCAGAACCT CATCAGTTGA TCCCAAACCA GGCTTCATCT 
TGTTCAACAC TAATGCCTCC ATCTTCTTTT GTTCCTCTGG TTCAAAGCAA GATAGACATA 
GCAAAAAGTG CTCTTCCACA CATTAGAGAA CAGCCTCAGA TAAAGAACCT CTCCAGCACA 
GAATTCTCCG ATTTTTCATG TCAGCTACTG TCACCAACTG ATTTCCTTCA AGGCATTGGG 
CCCCAGGCCC AAAGTGGTAC TACTGCAATT CAAATGGGAA AAAGTCCAGA GAAACCAAAA 
GAGAGCAAAC AGACTGATAC CCTTAAAAAT GAACTTCTAG AGATTCAAAG AATTATGCAG 
CAATACCAAG ACCAGAAAGA GGAGCTCAAG GATTGGCGCA GACATGCAGG AGTGCTACGC 
AGGTGGCTGA AAACCAGTGA CCATGTGCTA GACCCTTATG AACAATCACT CAGAAACGAA 
GTGCAGACTG AACTGCAGCA GCTCGAAGTG AAGATTGAAG AGCACTCACA GCTGCTAATC 
TCAAACAAGA GACAAATGCA GCTTTATATT AGTCGCCTTC AGGAGATCAG AGCATCAATG 
GACTTATTGT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	sfi1
Entrez Gene ID	100380177
Full Name	SFI1 centrin binding protein
General protein information	Preferred Names SFI1 centrin binding protein Names protein SFI1 homolog Sfi1 homolog, spindle assembly associated
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Mus musculus (house mouse)	Sfi1	NP_084483.2
Danio rerio (zebrafish)	sfi1	XP_688629.5
Pan troglodytes (chimpanzee)	SFI1	XP_001148452.1
	LOC716833	XP_001111056.1
Canis lupus familiaris (dog)	SFI1	XP_005636542.1
Bos taurus (cattle)	SFI1	XP_005218239.1
Rattus norvegicus (Norway rat)	Sfi1	NP_001178611.1
Homo sapiens (human)	SFI1	NP_001007468.1
Gallus gallus (chicken)	SFI1	XP_004934420.1
Xenopus tropicalis (tropical clawed frog)	sfi1	XP_004910651.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

sfi1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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