Sfi1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sfi1
Entrez Gene ID	78887
Full Name	Sfi1 homolog, spindle assembly associated (yeast)
General protein information	Preferred Names Sfi1 homolog, spindle assembly associated (yeast) Names protein SFI1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314845.1	XP_017170334.1	protein SFI1 homolog isoform X15
XM_006514894.3	XP_006514957.1	protein SFI1 homolog isoform X1
XM_006514896.3	XP_006514959.1	protein SFI1 homolog isoform X2
XM_006514898.3	XP_006514961.1	protein SFI1 homolog isoform X4
XM_006514900.3	XP_006514963.1	protein SFI1 homolog isoform X5
XM_006514902.4	XP_006514965.1	protein SFI1 homolog isoform X7
XM_030246433.1	XP_030102293.1	protein SFI1 homolog isoform X10
XM_006514908.3	XP_006514971.1	protein SFI1 homolog isoform X12
XM_017314842.1	XP_017170331.1	protein SFI1 homolog isoform X1
XM_006514902.3	XP_006514965.1	protein SFI1 homolog isoform X7
XM_011243794.2	XP_011242096.1	protein SFI1 homolog isoform X11
XM_017314842.2	XP_017170331.1	protein SFI1 homolog isoform X1
XM_006514901.3	XP_006514964.1	protein SFI1 homolog isoform X6
XM_030246434.1	XP_030102294.1	protein SFI1 homolog isoform X13
XM_017314844.1	XP_017170333.1	protein SFI1 homolog isoform X13
XM_011243798.1	XP_011242100.1	protein SFI1 homolog isoform X14
NM_030207.3	NP_084483.2	protein SFI1 homolog isoform 1
XM_017314846.1	XP_017170335.1	protein SFI1 homolog isoform X17
XM_006514903.3	XP_006514966.1	protein SFI1 homolog isoform X8
XM_006514904.3	XP_006514967.1	protein SFI1 homolog isoform X9
XM_011243797.2	XP_011242099.1	protein SFI1 homolog isoform X16
XM_006514903.4	XP_006514966.1	protein SFI1 homolog isoform X8
XM_011243792.2	XP_011242094.1	protein SFI1 homolog isoform X9
XM_011243797.3	XP_011242099.1	protein SFI1 homolog isoform X14
NM_001363223.1	NP_001350152.1	protein SFI1 homolog isoform 3
XM_017314843.1	XP_017170332.1	protein SFI1 homolog isoform X13
XM_017314847.1	XP_017170336.1	protein SFI1 homolog isoform X18
XM_017314841.1	XP_017170330.1	protein SFI1 homolog isoform X1
XM_006514897.3	XP_006514960.1	protein SFI1 homolog isoform X3
XM_030246435.1	XP_030102295.1	protein SFI1 homolog isoform X15
NM_001363222.1	NP_001350151.1	protein SFI1 homolog isoform 2
NM_030207.2	NP_084483.2	protein SFI1 homolog
NM_001363224.1	NP_001350153.1	protein SFI1 homolog isoform 4
XM_006514899.3	XP_006514962.1	protein SFI1 homolog isoform X5
XM_011243795.2	XP_011242097.1	protein SFI1 homolog isoform X11
XM_017314847.2	XP_017170336.1	protein SFI1 homolog isoform X16

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Sfi1	NP_084483.2
Danio rerio (zebrafish)	sfi1	XP_688629.5
Pan troglodytes (chimpanzee)	SFI1	XP_001148452.1
	LOC716833	XP_001111056.1
Canis lupus familiaris (dog)	SFI1	XP_005636542.1
Bos taurus (cattle)	SFI1	XP_005218239.1
Rattus norvegicus (Norway rat)	Sfi1	NP_001178611.1
Homo sapiens (human)	SFI1	NP_001007468.1
Gallus gallus (chicken)	SFI1	XP_004934420.1
Xenopus tropicalis (tropical clawed frog)	sfi1	XP_004910651.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread and dynamic translational control of red blood cell development.
Alvarez-Dominguez JR, Zhang X, Hu W
Blood129(5)619-629(2017 02)

Divergence patterns of genic copy number variation in natural populations of the house mouse (Mus musculus domesticus) reveal three conserved genes with major population-specific expansions.
Pezer ?, Harr B, Teschke M, Babiker H, Tautz D
Genome research25(8)1114-24(2015 Aug)

Global discovery of erythroid long noncoding RNAs reveals novel regulators of red cell maturation.
Alvarez-Dominguez JR, Hu W, Yuan B, Shi J, Park SS, Gromatzky AA, van Oudenaarden A, Lodish HF
Blood123(4)570-81(2014 Jan)

The Vertebrate Genome Annotation browser 10 years on.
Harrow JL, Steward CA, Frankish A, Gilbert JG, Gonzalez JM, Loveland JE, Mudge J, Sheppard D, Thomas M, Trevanion S, Wilming LG
Nucleic acids research42(Database issue)D771-9(2014 Jan)

Genome-wide analysis of H4K5 acetylation associated with fear memory in mice.
Park CS, Rehrauer H, Mansuy IM
BMC genomics14539(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study showed major differences in gene copy number in natural populations. CWC22, SFI1, and HJURP are highly conserved genes with the most copy-number variation. They all exhibit population-specific expansion patterns, perhaps for local adaptations.
Title: Divergence patterns of genic copy number variation in natural populations of the house mouse (Mus musculus domesticus) reveal three conserved genes with major population-specific expansions.

The following Sfi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sfi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu45329	NM_001363222.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu45325	XM_006514904.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu45329	XM_006514908.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu45329	XM_017314843.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71773	XM_011243792.2 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu71775	XM_011243794.2 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71776	XM_011243795.2 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71777	XM_017314844.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu71778	XM_011243798.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu78085	NM_001363223.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu78086	NM_001363224.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu71775	XM_030246433.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu71777	XM_030246434.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu71778	XM_011243797.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu78085	XM_017314845.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu78086	XM_017314846.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu78087	XM_017314847.2 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu85949	XM_030246435.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu45318	XM_017314842.2 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu45323	XM_006514902.4 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu45324	XM_006514903.4 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu13878	NM_030207.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu13878	XM_006514896.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu45318	XM_017314841.1 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu45318	XM_006514894.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu45319	XM_006514897.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu45320	XM_006514898.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu45321	XM_006514899.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu45321	XM_006514900.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu45322	XM_006514901.3 Latest version!	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu45323	XM_006514902.3	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu45318	XM_017314842.1	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu45324	XM_006514903.3	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu71778	XM_011243797.2	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu13878	NM_030207.2	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu78087	XM_017314847.1	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sfi1 ( NM_001363222.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350151.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu45329
Clone ID Related Accession (Same CDS sequence)	XM_006514908.3 , XM_017314843.1 , NM_001363222.1
Accession Version	NM_001363222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671968.17 and BX572640.9. On May 12, 2018 this sequence version replaced XM_017314843.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.80695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTA AGACCAGGTC AGCCCAGGGC CAGCCCCTGG 
CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT CCCTCTCGCC CATGTCCCCA GGTGGAACTA 
CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG GCCCAGCGTC AGCCTGTTGG TACATGCATT 
GCTCGTGTTC GGGCCCTGAG ACGGGCACTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350151.1
CDS	2233..4608
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	NM_001363222.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363222.1

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTA AGACCAGGTC AGCCCAGGGC CAGCCCCTGG 
CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT CCCTCTCGCC CATGTCCCCA GGTGGAACTA 
CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG GCCCAGCGTC AGCCTGTTGG TACATGCATT 
GCTCGTGTTC GGGCCCTGAG ACGGGCACTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514904.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514967.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu45325
Clone ID Related Accession (Same CDS sequence)	XM_006514904.3
Accession Version	XM_006514904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG CAATGCAGCC CGCTCATCAC GGAAACAGCC 
CCGACGTCCA TCCTTCCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514967.1
CDS	600..3323
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQQCSPLITETAPTSILPA

Target ORF information:

RefSeq Version	XM_006514904.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514904.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG CAATGCAGCC CGCTCATCAC GGAAACAGCC 
CCGACGTCCA TCCTTCCTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514908.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514971.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu45329
Clone ID Related Accession (Same CDS sequence)	XM_006514908.3 , XM_017314843.1 , NM_001363222.1
Accession Version	XM_006514908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTA AGACCAGGTC AGCCCAGGGC CAGCCCCTGG 
CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT CCCTCTCGCC CATGTCCCCA GGTGGAACTA 
CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG GCCCAGCGTC AGCCTGTTGG TACATGCATT 
GCTCGTGTTC GGGCCCTGAG ACGGGCACTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514971.1
CDS	1433..3808
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514908.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514908.3

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTA AGACCAGGTC AGCCCAGGGC CAGCCCCTGG 
CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT CCCTCTCGCC CATGTCCCCA GGTGGAACTA 
CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG GCCCAGCGTC AGCCTGTTGG TACATGCATT 
GCTCGTGTTC GGGCCCTGAG ACGGGCACTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314843.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170332.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu45329
Clone ID Related Accession (Same CDS sequence)	XM_006514908.3 , XM_017314843.1 , NM_001363222.1
Accession Version	XM_017314843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTA AGACCAGGTC AGCCCAGGGC CAGCCCCTGG 
CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT CCCTCTCGCC CATGTCCCCA GGTGGAACTA 
CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG GCCCAGCGTC AGCCTGTTGG TACATGCATT 
GCTCGTGTTC GGGCCCTGAG ACGGGCACTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170332.1
CDS	2054..4429
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314843.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314843.1

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTA AGACCAGGTC AGCCCAGGGC CAGCCCCTGG 
CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT CCCTCTCGCC CATGTCCCCA GGTGGAACTA 
CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG GCCCAGCGTC AGCCTGTTGG TACATGCATT 
GCTCGTGTTC GGGCCCTGAG ACGGGCACTG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_011243792.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242094.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X16, mRNA.

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CloneID	OMu71773
Clone ID Related Accession (Same CDS sequence)	XM_011243792.2
Accession Version	XM_011243792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTTC ACAGGTTCTG GAACCTCTGG CAGTCTCGAA TTGAGCAGAG GGAGGAGAGG 
GTACAGACCC CCTCATTGCA TGCAGCCCTG AGCCACTACA GGGTGACAGT CCTACACAAG 
TGTGTCAGGG TGTGGCTACG GTATGTGCAC AAGAGACAAT GGCAGCAGTT ATTGCGGGCC 
AGAGCAGATG GTCATTTCCA GCAGCGAGCA CTGCCTGCTG CCTTCTACAC GTGGTACAGA 
GGCTGGCTAT GGCACCAGCA GAGACGGATC CTGCACACAA AAGCAGTGCG TTTCCACAGA 
GGGACATTAG AGAAGCAAGT ATTTGCTCTC TGGAGGCAGA AGATGTCTCA GCATCGAGAA 
AACTGCTTGG CAGAGAGAAT GGCTATACTG CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC 
TGGTTCGTGT GGCACCAGCA GGCAGCAGTG TGTCAGCTGG AGCGACAGCA GCAAGCCATG 
GCCATTGCCC ACCACCACAG TGGACTGCTC AGGAGGGCTT TCTGCATCTG GAAGGAAAGC 
ACCCAAGGAT TTAGGATAGA GAGGATGGGC AGGGCACAGG CTGCGCACTT CCACTCTGCA 
CAGCTCTTGA GTCGGGCCTG GAGCATGTGG AGGGAGTGCC TGGCCCTGAG GCTGGAAGAG 
CAGCAGAAAC TGAAGTGTGC GGCCCTGCAC AGCCAGTGCA TCCTGCTTCG CAGGGCTCTG 
CAGAAGTGGC TGGTTTACCA GAATAGGGTG AGGAGTGTTC TGCGGGAGGT GGCGGCCAGG 
GAGAGACAGC ACAACAGGCA GCTGCTGTGG TGGGCGTTAC ATCTCTGGAG AGAAAACACC 
ATGGCCCGTT TGGATGGAGC CAAAAAAACC TCTCAAGCAA GAGTTCACTA CAGCAGGACT 
CTGTGCTCCA AGGTCCTCGT CCAGTGGCGA GAGGTTACTT CAGTGCAGAT ATATTACCGA 
CAAAAGGAGG CAGCTGCCCT CAGGGAGGCC CGGAAAGCAC TGGACAGGGG TCGTCTCCAG 
AACTGGTTTC AGCACTGGCG ATTCTGCAGC CAGAGGGCAG CCCAGCAGAG ATTCCAGCTG 
GGACAGGCGG CTCAGCATCA CCACTGGCAG CTGCTGATGG AAGCAATGGC CAGGTGGAAG 
GCACACCATC TGGGGTGTAT CAGAAAGAAG TTTCTGCAGA GGCAAGCTGC CCAACTCCTG 
GCACAGAGAC TCAGTCGGGC CTGCTTCTGC CAGTGGAGGA AGCAGCTGGC AGTCCGGAAG 
CAAGAACAGT GGGGCACAGC CCGGGCCCTG TGGCTCTGGG CCTTCTCACT ACAAGCAAAG 
GTGTGGACTG CATGGCTGGG CTTCGTACTT GAGAGGAGGA GGAAGAAGGC ACGGCTGGAA 
CGGGCTATGC AAGCCTACCA GCAGCAGCTC CTACAGGAGG GTGCCACACG GCTTCTGCGC 
TTCACAGCTG GTACGAAGGC CTTCCGGCAG CAGCTCCAGG CCCAGCAGCA GGTCCAGGCA 
GCCCACAGCC TCCACTGTGC AGTCCGCCAC TGTGCTGAGC TCTGGAAAAA GAAGGTGCTT 
GGTCCAGGCA AGACTTCTCA ACCACCAGCA CCCACCACAT TCAGCAAGAG AGTGACATTT 
AAGGATTCTT TCCTCTCTGG ACATGCTGCG GAGGCTGGAG ATGCTACCCA GGAGACAAAG 
AAGCTTCGAG CTCCTCCATC TCAGGGAGTC CTGGGTAGTT TGGCAGGAGC TGCTGGGGAG 
CCCTGCCACC TAGACCTCAA TGCAGCCCGC TCATCACGGA AACAGCCCCG ACGTCCATCC 
TTCCTGCTTG AGCGTTTGGG GAGTCAGAGG TCCCCAGAGT GGTACAGCCT TGGGGAGCAG 
CAGTTAGAGA AGCCCCCAGA GGAGGAAAGC ACGGCTCTGC TGGGAGGCTC TTCCCTGACA 
AGACCCTTCC TACCTGGGGT CCTGCCGAAT GTTCCTGGTC CGAAGCTGCC TCCTACGGCC 
AGTCCAGGCC TGGAGCTGCT GCCTCCCTCC TCCATCATGC CCCATGCGGC AGGAGGCACT 
GCTAGGGTGT CAGCGAAGCC CAGCATCCCT GGGCCTCAGC CTTGGGGGTG CCCGTCCTTA 
CCCAGGGACC TTGATCCCCA ACTCCTCCCT GGAGATTCCA TAAGCACCCG GACTGAGCCT 
GTTTATGGCT CAGAGGCTAC AGGCCACACA GAGCTTGAGG CTGAGTTGGA GGGGATCCAG 
CAGCAGTTAC AACACTACCA AACCACCAAG CAGAACCTTT GGTCATGCCA GCGACAAGCC 
AACAGCCTGC GTAGGTGGCT GGAGCTAAGC CAGGAGGAGC CGAAATCCGA GGACCTTCAC 
CTAGAAGAGC AGGTGAAAAC AGAACTGGAG GAGGTAAGAC CAGGTCAGCC CAGGGCCAGC 
CCCTGGCTGT CCTTCCTTTC AGCTTGCCTT GTCCCTCCCT CTCGCCCATG TCCCCAGGTG 
GAACTACAGG TCCAGCAGCT GGCTAAGGAG CTTGAGGCCC AGCGTCAGCC TGTTGGTACA 
TGCATTGCTC GTGTTCGGGC CCTGAGACGG GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242094.1
CDS	1235..3796
Translation	MLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_011243792.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243792.2

ORF Insert Sequence:

ATGTTGCTTC ACAGGTTCTG GAACCTCTGG CAGTCTCGAA TTGAGCAGAG GGAGGAGAGG 
GTACAGACCC CCTCATTGCA TGCAGCCCTG AGCCACTACA GGGTGACAGT CCTACACAAG 
TGTGTCAGGG TGTGGCTACG GTATGTGCAC AAGAGACAAT GGCAGCAGTT ATTGCGGGCC 
AGAGCAGATG GTCATTTCCA GCAGCGAGCA CTGCCTGCTG CCTTCTACAC GTGGTACAGA 
GGCTGGCTAT GGCACCAGCA GAGACGGATC CTGCACACAA AAGCAGTGCG TTTCCACAGA 
GGGACATTAG AGAAGCAAGT ATTTGCTCTC TGGAGGCAGA AGATGTCTCA GCATCGAGAA 
AACTGCTTGG CAGAGAGAAT GGCTATACTG CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC 
TGGTTCGTGT GGCACCAGCA GGCAGCAGTG TGTCAGCTGG AGCGACAGCA GCAAGCCATG 
GCCATTGCCC ACCACCACAG TGGACTGCTC AGGAGGGCTT TCTGCATCTG GAAGGAAAGC 
ACCCAAGGAT TTAGGATAGA GAGGATGGGC AGGGCACAGG CTGCGCACTT CCACTCTGCA 
CAGCTCTTGA GTCGGGCCTG GAGCATGTGG AGGGAGTGCC TGGCCCTGAG GCTGGAAGAG 
CAGCAGAAAC TGAAGTGTGC GGCCCTGCAC AGCCAGTGCA TCCTGCTTCG CAGGGCTCTG 
CAGAAGTGGC TGGTTTACCA GAATAGGGTG AGGAGTGTTC TGCGGGAGGT GGCGGCCAGG 
GAGAGACAGC ACAACAGGCA GCTGCTGTGG TGGGCGTTAC ATCTCTGGAG AGAAAACACC 
ATGGCCCGTT TGGATGGAGC CAAAAAAACC TCTCAAGCAA GAGTTCACTA CAGCAGGACT 
CTGTGCTCCA AGGTCCTCGT CCAGTGGCGA GAGGTTACTT CAGTGCAGAT ATATTACCGA 
CAAAAGGAGG CAGCTGCCCT CAGGGAGGCC CGGAAAGCAC TGGACAGGGG TCGTCTCCAG 
AACTGGTTTC AGCACTGGCG ATTCTGCAGC CAGAGGGCAG CCCAGCAGAG ATTCCAGCTG 
GGACAGGCGG CTCAGCATCA CCACTGGCAG CTGCTGATGG AAGCAATGGC CAGGTGGAAG 
GCACACCATC TGGGGTGTAT CAGAAAGAAG TTTCTGCAGA GGCAAGCTGC CCAACTCCTG 
GCACAGAGAC TCAGTCGGGC CTGCTTCTGC CAGTGGAGGA AGCAGCTGGC AGTCCGGAAG 
CAAGAACAGT GGGGCACAGC CCGGGCCCTG TGGCTCTGGG CCTTCTCACT ACAAGCAAAG 
GTGTGGACTG CATGGCTGGG CTTCGTACTT GAGAGGAGGA GGAAGAAGGC ACGGCTGGAA 
CGGGCTATGC AAGCCTACCA GCAGCAGCTC CTACAGGAGG GTGCCACACG GCTTCTGCGC 
TTCACAGCTG GTACGAAGGC CTTCCGGCAG CAGCTCCAGG CCCAGCAGCA GGTCCAGGCA 
GCCCACAGCC TCCACTGTGC AGTCCGCCAC TGTGCTGAGC TCTGGAAAAA GAAGGTGCTT 
GGTCCAGGCA AGACTTCTCA ACCACCAGCA CCCACCACAT TCAGCAAGAG AGTGACATTT 
AAGGATTCTT TCCTCTCTGG ACATGCTGCG GAGGCTGGAG ATGCTACCCA GGAGACAAAG 
AAGCTTCGAG CTCCTCCATC TCAGGGAGTC CTGGGTAGTT TGGCAGGAGC TGCTGGGGAG 
CCCTGCCACC TAGACCTCAA TGCAGCCCGC TCATCACGGA AACAGCCCCG ACGTCCATCC 
TTCCTGCTTG AGCGTTTGGG GAGTCAGAGG TCCCCAGAGT GGTACAGCCT TGGGGAGCAG 
CAGTTAGAGA AGCCCCCAGA GGAGGAAAGC ACGGCTCTGC TGGGAGGCTC TTCCCTGACA 
AGACCCTTCC TACCTGGGGT CCTGCCGAAT GTTCCTGGTC CGAAGCTGCC TCCTACGGCC 
AGTCCAGGCC TGGAGCTGCT GCCTCCCTCC TCCATCATGC CCCATGCGGC AGGAGGCACT 
GCTAGGGTGT CAGCGAAGCC CAGCATCCCT GGGCCTCAGC CTTGGGGGTG CCCGTCCTTA 
CCCAGGGACC TTGATCCCCA ACTCCTCCCT GGAGATTCCA TAAGCACCCG GACTGAGCCT 
GTTTATGGCT CAGAGGCTAC AGGCCACACA GAGCTTGAGG CTGAGTTGGA GGGGATCCAG 
CAGCAGTTAC AACACTACCA AACCACCAAG CAGAACCTTT GGTCATGCCA GCGACAAGCC 
AACAGCCTGC GTAGGTGGCT GGAGCTAAGC CAGGAGGAGC CGAAATCCGA GGACCTTCAC 
CTAGAAGAGC AGGTGAAAAC AGAACTGGAG GAGGTAAGAC CAGGTCAGCC CAGGGCCAGC 
CCCTGGCTGT CCTTCCTTTC AGCTTGCCTT GTCCCTCCCT CTCGCCCATG TCCCCAGGTG 
GAACTACAGG TCCAGCAGCT GGCTAAGGAG CTTGAGGCCC AGCGTCAGCC TGTTGGTACA 
TGCATTGCTC GTGTTCGGGC CCTGAGACGG GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_011243794.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242096.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu71775
Clone ID Related Accession (Same CDS sequence)	XM_030246433.1 , XM_011243794.2
Accession Version	XM_011243794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGTGCC TGGCCCTGAG GCTGGAAGAG CAGCAGAAAC TGAAGTGTGC GGCCCTGCAC 
AGCCAGTGCA TCCTGCTTCG CAGGGCTCTG CAGAAGTGGC TGGTTTACCA GAATAGGGTG 
AGGAGTGTTC TGCGGGAGGT GGCGGCCAGG GAGAGACAGC ACAACAGGCA GCTGCTGTGG 
TGGGCGTTAC ATCTCTGGAG AGAAAACACC ATGGCCCGTT TGGATGGAGC CAAAAAAACC 
TCTCAAGCAA GAGTTCACTA CAGCAGGACT CTGTGCTCCA AGGTCCTCGT CCAGTGGCGA 
GAGGTTACTT CAGTGCAGAT ATATTACCGA CAAAAGGAGG CAGCTGCCCT CAGGGAGGCC 
CGGAAAGCAC TGGACAGGGG TCGTCTCCAG AACTGGTTTC AGCACTGGCG ATTCTGCAGC 
CAGAGGGCAG CCCAGCAGAG ATTCCAGCTG GGACAGGCGG CTCAGCATCA CCACTGGCAG 
CTGCTGATGG AAGCAATGGC CAGGTGGAAG GCACACCATC TGGGGTGTAT CAGAAAGAAG 
TTTCTGCAGA GGCAAGCTGC CCAACTCCTG GCACAGAGAC TCAGTCGGGC CTGCTTCTGC 
CAGTGGAGGA AGCAGCTGGC AGTCCGGAAG CAAGAACAGT GGGGCACAGC CCGGGCCCTG 
TGGCTCTGGG CCTTCTCACT ACAAGCAAAG GTGTGGACTG CATGGCTGGG CTTCGTACTT 
GAGAGGAGGA GGAAGAAGGC ACGGCTGGAA CGGGCTATGC AAGCCTACCA GCAGCAGCTC 
CTACAGGAGG GTGCCACACG GCTTCTGCGC TTCACAGCTG GTACGAAGGC CTTCCGGCAG 
CAGCTCCAGG CCCAGCAGCA GGTCCAGGCA GCCCACAGCC TCCACTGTGC AGTCCGCCAC 
TGTGCTGAGC TCTGGAAAAA GAAGGTGCTT GGTCCAGGCA AGACTTCTCA ACCACCAGCA 
CCCACCACAT TCAGCAAGAG AGTGACATTT AAGGATTCTT TCCTCTCTGG ACATGCTGCG 
GAGGCTGGAG ATGCTACCCA GGAGACAAAG AAGCTTCGAG CTCCTCCATC TCAGGGAGTC 
CTGGGTAGTT TGGCAGGAGC TGCTGGGGAG CCCTGCCACC TAGACCTCAA TGCAGCCCGC 
TCATCACGGA AACAGCCCCG ACGTCCATCC TTCCTGCTTG AGCGTTTGGG GAGTCAGAGG 
TCCCCAGAGT GGTACAGCCT TGGGGAGCAG CAGTTAGAGA AGCCCCCAGA GGAGGAAAGC 
ACGGCTCTGC TGGGAGGCTC TTCCCTGACA AGACCCTTCC TACCTGGGGT CCTGCCGAAT 
GTTCCTGGTC CGAAGCTGCC TCCTACGGCC AGTCCAGGCC TGGAGCTGCT GCCTCCCTCC 
TCCATCATGC CCCATGCGGC AGGAGGCACT GCTAGGGTGT CAGCGAAGCC CAGCATCCCT 
GGGCCTCAGC CTTGGGGGTG CCCGTCCTTA CCCAGGGACC TTGATCCCCA ACTCCTCCCT 
GGAGATTCCA TAAGCACCCG GACTGAGCCT GTTTATGGCT CAGAGGCTAC AGGCCACACA 
GAGCTTGAGG CTGAGTTGGA GGGGATCCAG CAGCAGTTAC AACACTACCA AACCACCAAG 
CAGAACCTTT GGTCATGCCA GCGACAAGCC AACAGCCTGC GTAGGTGGCT GGAGCTAAGC 
CAGGAGGAGC CGAAATCCGA GGACCTTCAC CTAGAAGAGC AGGTGAAAAC AGAACTGGAG 
GAGGTAAGAC CAGGTCAGCC CAGGGCCAGC CCCTGGCTGT CCTTCCTTTC AGCTTGCCTT 
GTCCCTCCCT CTCGCCCATG TCCCCAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242096.1
CDS	1227..3698
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_011243794.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243794.2

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGTGCC TGGCCCTGAG GCTGGAAGAG CAGCAGAAAC TGAAGTGTGC GGCCCTGCAC 
AGCCAGTGCA TCCTGCTTCG CAGGGCTCTG CAGAAGTGGC TGGTTTACCA GAATAGGGTG 
AGGAGTGTTC TGCGGGAGGT GGCGGCCAGG GAGAGACAGC ACAACAGGCA GCTGCTGTGG 
TGGGCGTTAC ATCTCTGGAG AGAAAACACC ATGGCCCGTT TGGATGGAGC CAAAAAAACC 
TCTCAAGCAA GAGTTCACTA CAGCAGGACT CTGTGCTCCA AGGTCCTCGT CCAGTGGCGA 
GAGGTTACTT CAGTGCAGAT ATATTACCGA CAAAAGGAGG CAGCTGCCCT CAGGGAGGCC 
CGGAAAGCAC TGGACAGGGG TCGTCTCCAG AACTGGTTTC AGCACTGGCG ATTCTGCAGC 
CAGAGGGCAG CCCAGCAGAG ATTCCAGCTG GGACAGGCGG CTCAGCATCA CCACTGGCAG 
CTGCTGATGG AAGCAATGGC CAGGTGGAAG GCACACCATC TGGGGTGTAT CAGAAAGAAG 
TTTCTGCAGA GGCAAGCTGC CCAACTCCTG GCACAGAGAC TCAGTCGGGC CTGCTTCTGC 
CAGTGGAGGA AGCAGCTGGC AGTCCGGAAG CAAGAACAGT GGGGCACAGC CCGGGCCCTG 
TGGCTCTGGG CCTTCTCACT ACAAGCAAAG GTGTGGACTG CATGGCTGGG CTTCGTACTT 
GAGAGGAGGA GGAAGAAGGC ACGGCTGGAA CGGGCTATGC AAGCCTACCA GCAGCAGCTC 
CTACAGGAGG GTGCCACACG GCTTCTGCGC TTCACAGCTG GTACGAAGGC CTTCCGGCAG 
CAGCTCCAGG CCCAGCAGCA GGTCCAGGCA GCCCACAGCC TCCACTGTGC AGTCCGCCAC 
TGTGCTGAGC TCTGGAAAAA GAAGGTGCTT GGTCCAGGCA AGACTTCTCA ACCACCAGCA 
CCCACCACAT TCAGCAAGAG AGTGACATTT AAGGATTCTT TCCTCTCTGG ACATGCTGCG 
GAGGCTGGAG ATGCTACCCA GGAGACAAAG AAGCTTCGAG CTCCTCCATC TCAGGGAGTC 
CTGGGTAGTT TGGCAGGAGC TGCTGGGGAG CCCTGCCACC TAGACCTCAA TGCAGCCCGC 
TCATCACGGA AACAGCCCCG ACGTCCATCC TTCCTGCTTG AGCGTTTGGG GAGTCAGAGG 
TCCCCAGAGT GGTACAGCCT TGGGGAGCAG CAGTTAGAGA AGCCCCCAGA GGAGGAAAGC 
ACGGCTCTGC TGGGAGGCTC TTCCCTGACA AGACCCTTCC TACCTGGGGT CCTGCCGAAT 
GTTCCTGGTC CGAAGCTGCC TCCTACGGCC AGTCCAGGCC TGGAGCTGCT GCCTCCCTCC 
TCCATCATGC CCCATGCGGC AGGAGGCACT GCTAGGGTGT CAGCGAAGCC CAGCATCCCT 
GGGCCTCAGC CTTGGGGGTG CCCGTCCTTA CCCAGGGACC TTGATCCCCA ACTCCTCCCT 
GGAGATTCCA TAAGCACCCG GACTGAGCCT GTTTATGGCT CAGAGGCTAC AGGCCACACA 
GAGCTTGAGG CTGAGTTGGA GGGGATCCAG CAGCAGTTAC AACACTACCA AACCACCAAG 
CAGAACCTTT GGTCATGCCA GCGACAAGCC AACAGCCTGC GTAGGTGGCT GGAGCTAAGC 
CAGGAGGAGC CGAAATCCGA GGACCTTCAC CTAGAAGAGC AGGTGAAAAC AGAACTGGAG 
GAGGTAAGAC CAGGTCAGCC CAGGGCCAGC CCCTGGCTGT CCTTCCTTTC AGCTTGCCTT 
GTCCCTCCCT CTCGCCCATG TCCCCAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_011243795.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242097.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu71776
Clone ID Related Accession (Same CDS sequence)	XM_011243795.2
Accession Version	XM_011243795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GTGCCTGGCC 
CTGAGGCTGG AAGAGCAGCA GAAACTGAAG TGTGCGGCCC TGCACAGCCA GTGCATCCTG 
CTTCGCAGGG CTCTGCAGAA GTGGCTGGTT TACCAGAATA GGGTGAGGAG TGTTCTGCGG 
GAGGTGGCGG CCAGGGAGAG ACAGCACAAC AGGCAGCTGC TGTGGTGGGC GTTACATCTC 
TGGAGAGAAA ACACCATGGC CCGTTTGGAT GGAGCCAAAA AAACCTCTCA AGCAAGAGTT 
CACTACAGCA GGACTCTGTG CTCCAAGGTC CTCGTCCAGT GGCGAGAGGT TACTTCAGTG 
CAGATATATT ACCGACAAAA GGAGGCAGCT GCCCTCAGGG AGGCCCGGAA AGCACTGGAC 
AGGGGTCGTC TCCAGAACTG GTTTCAGCAC TGGCGATTCT GCAGCCAGAG GGCAGCCCAG 
CAGAGATTCC AGCTGGGACA GGCGGCTCAG CATCACCACT GGCAGCTGCT GATGGAAGCA 
ATGGCCAGGT GGAAGGCACA CCATCTGGGG TGTATCAGAA AGAAGTTTCT GCAGAGGCAA 
GCTGCCCAAC TCCTGGCACA GAGACTCAGT CGGGCCTGCT TCTGCCAGTG GAGGAAGCAG 
CTGGCAGTCC GGAAGCAAGA ACAGTGGGGC ACAGCCCGGG CCCTGTGGCT CTGGGCCTTC 
TCACTACAAG CAAAGGTGTG GACTGCATGG CTGGGCTTCG TACTTGAGAG GAGGAGGAAG 
AAGGCACGGC TGGAACGGGC TATGCAAGCC TACCAGCAGC AGCTCCTACA GGAGGGTGCC 
ACACGGCTTC TGCGCTTCAC AGCTGGTACG AAGGCCTTCC GGCAGCAGCT CCAGGCCCAG 
CAGCAGGTCC AGGCAGCCCA CAGCCTCCAC TGTGCAGTCC GCCACTGTGC TGAGCTCTGG 
AAAAAGAAGG TGCTTGGTCC AGGCAAGACT TCTCAACCAC CAGCACCCAC CACATTCAGC 
AAGAGAGTGA CATTTAAGGA TTCTTTCCTC TCTGGACATG CTGCGGAGGC TGGAGATGCT 
ACCCAGGAGA CAAAGAAGCT TCGAGCTCCT CCATCTCAGG GAGTCCTGGG TAGTTTGGCA 
GGAGCTGCTG GGGAGCCCTG CCACCTAGAC CTCAATGCAG CCCGCTCATC ACGGAAACAG 
CCCCGACGTC CATCCTTCCT GCTTGAGCGT TTGGGGAGTC AGAGGTCCCC AGAGTGGTAC 
AGCCTTGGGG AGCAGCAGTT AGAGAAGCCC CCAGAGGAGG AAAGCACGGC TCTGCTGGGA 
GGCTCTTCCC TGACAAGACC CTTCCTACCT GGGGTCCTGC CGAATGTTCC TGGTCCGAAG 
CTGCCTCCTA CGGCCAGTCC AGGCCTGGAG CTGCTGCCTC CCTCCTCCAT CATGCCCCAT 
GCGGCAGGAG GCACTGCTAG GGTGTCAGCG AAGCCCAGCA TCCCTGGGCC TCAGCCTTGG 
GGGTGCCCGT CCTTACCCAG GGACCTTGAT CCCCAACTCC TCCCTGGAGA TTCCATAAGC 
ACCCGGACTG AGCCTGTTTA TGGCTCAGAG GCTACAGGCC ACACAGAGCT TGAGGCTGAG 
TTGGAGGGGA TCCAGCAGCA GTTACAACAC TACCAAACCA CCAAGCAGAA CCTTTGGTCA 
TGCCAGCGAC AAGCCAACAG CCTGCGTAGG TGGCTGGAGC TAAGCCAGGA GGAGCCGAAA 
TCCGAGGACC TTCACCTAGA AGAGCAGGTG AAAACAGAAC TGGAGGAGGT AAGACCAGGT 
CAGCCCAGGG CCAGCCCCTG GCTGTCCTTC CTTTCAGCTT GCCTTGTCCC TCCCTCTCGC 
CCATGTCCCC AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242097.1
CDS	1235..3631
Translation	MLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_011243795.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243795.2

ORF Insert Sequence:

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GTGCCTGGCC 
CTGAGGCTGG AAGAGCAGCA GAAACTGAAG TGTGCGGCCC TGCACAGCCA GTGCATCCTG 
CTTCGCAGGG CTCTGCAGAA GTGGCTGGTT TACCAGAATA GGGTGAGGAG TGTTCTGCGG 
GAGGTGGCGG CCAGGGAGAG ACAGCACAAC AGGCAGCTGC TGTGGTGGGC GTTACATCTC 
TGGAGAGAAA ACACCATGGC CCGTTTGGAT GGAGCCAAAA AAACCTCTCA AGCAAGAGTT 
CACTACAGCA GGACTCTGTG CTCCAAGGTC CTCGTCCAGT GGCGAGAGGT TACTTCAGTG 
CAGATATATT ACCGACAAAA GGAGGCAGCT GCCCTCAGGG AGGCCCGGAA AGCACTGGAC 
AGGGGTCGTC TCCAGAACTG GTTTCAGCAC TGGCGATTCT GCAGCCAGAG GGCAGCCCAG 
CAGAGATTCC AGCTGGGACA GGCGGCTCAG CATCACCACT GGCAGCTGCT GATGGAAGCA 
ATGGCCAGGT GGAAGGCACA CCATCTGGGG TGTATCAGAA AGAAGTTTCT GCAGAGGCAA 
GCTGCCCAAC TCCTGGCACA GAGACTCAGT CGGGCCTGCT TCTGCCAGTG GAGGAAGCAG 
CTGGCAGTCC GGAAGCAAGA ACAGTGGGGC ACAGCCCGGG CCCTGTGGCT CTGGGCCTTC 
TCACTACAAG CAAAGGTGTG GACTGCATGG CTGGGCTTCG TACTTGAGAG GAGGAGGAAG 
AAGGCACGGC TGGAACGGGC TATGCAAGCC TACCAGCAGC AGCTCCTACA GGAGGGTGCC 
ACACGGCTTC TGCGCTTCAC AGCTGGTACG AAGGCCTTCC GGCAGCAGCT CCAGGCCCAG 
CAGCAGGTCC AGGCAGCCCA CAGCCTCCAC TGTGCAGTCC GCCACTGTGC TGAGCTCTGG 
AAAAAGAAGG TGCTTGGTCC AGGCAAGACT TCTCAACCAC CAGCACCCAC CACATTCAGC 
AAGAGAGTGA CATTTAAGGA TTCTTTCCTC TCTGGACATG CTGCGGAGGC TGGAGATGCT 
ACCCAGGAGA CAAAGAAGCT TCGAGCTCCT CCATCTCAGG GAGTCCTGGG TAGTTTGGCA 
GGAGCTGCTG GGGAGCCCTG CCACCTAGAC CTCAATGCAG CCCGCTCATC ACGGAAACAG 
CCCCGACGTC CATCCTTCCT GCTTGAGCGT TTGGGGAGTC AGAGGTCCCC AGAGTGGTAC 
AGCCTTGGGG AGCAGCAGTT AGAGAAGCCC CCAGAGGAGG AAAGCACGGC TCTGCTGGGA 
GGCTCTTCCC TGACAAGACC CTTCCTACCT GGGGTCCTGC CGAATGTTCC TGGTCCGAAG 
CTGCCTCCTA CGGCCAGTCC AGGCCTGGAG CTGCTGCCTC CCTCCTCCAT CATGCCCCAT 
GCGGCAGGAG GCACTGCTAG GGTGTCAGCG AAGCCCAGCA TCCCTGGGCC TCAGCCTTGG 
GGGTGCCCGT CCTTACCCAG GGACCTTGAT CCCCAACTCC TCCCTGGAGA TTCCATAAGC 
ACCCGGACTG AGCCTGTTTA TGGCTCAGAG GCTACAGGCC ACACAGAGCT TGAGGCTGAG 
TTGGAGGGGA TCCAGCAGCA GTTACAACAC TACCAAACCA CCAAGCAGAA CCTTTGGTCA 
TGCCAGCGAC AAGCCAACAG CCTGCGTAGG TGGCTGGAGC TAAGCCAGGA GGAGCCGAAA 
TCCGAGGACC TTCACCTAGA AGAGCAGGTG AAAACAGAAC TGGAGGAGGT AAGACCAGGT 
CAGCCCAGGG CCAGCCCCTG GCTGTCCTTC CTTTCAGCTT GCCTTGTCCC TCCCTCTCGC 
CCATGTCCCC AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314844.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170333.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OMu71777
Clone ID Related Accession (Same CDS sequence)	XM_030246434.1 , XM_017314844.1
Accession Version	XM_017314844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTAAGACC AGGTCAGCCC AGGGCCAGCC CCTGGCTGTC CTTCCTTTCA 
GCTTGCCTTG TCCCTCCCTC TCGCCCATGT CCCCAGGTGG AACTACAGGT CCAGCAGCTG 
GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT GTTGGTACAT GCATTGCTCG TGTTCGGGCC 
CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170333.1
CDS	1504..3804
Translation	MLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314844.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314844.1

ORF Insert Sequence:

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTAAGACC AGGTCAGCCC AGGGCCAGCC CCTGGCTGTC CTTCCTTTCA 
GCTTGCCTTG TCCCTCCCTC TCGCCCATGT CCCCAGGTGG AACTACAGGT CCAGCAGCTG 
GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT GTTGGTACAT GCATTGCTCG TGTTCGGGCC 
CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_011243798.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242100.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X24, mRNA.

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CloneID	OMu71778
Clone ID Related Accession (Same CDS sequence)	XM_011243798.1 , XM_011243797.2 , XM_011243797.3
Accession Version	XM_011243798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242100.1
CDS	128..2416
Translation	MCTRDNGSRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_011243798.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243798.1

ORF Insert Sequence:

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_011243797.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242099.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OMu71778
Clone ID Related Accession (Same CDS sequence)	XM_011243798.1 , XM_011243797.2 , XM_011243797.3
Accession Version	XM_011243797.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011243797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242099.1
CDS	2323..4611
Translation	MCTRDNGSRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_011243797.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243797.2

ORF Insert Sequence:

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( NM_001363223.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350152.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 3, mRNA.

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CloneID	OMu78085
Clone ID Related Accession (Same CDS sequence)	XM_017314845.1 , NM_001363223.1
Accession Version	NM_001363223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671968.17 and BX572640.9. On May 12, 2018 this sequence version replaced XM_017314845.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.154085.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350152.1
CDS	2170..4461
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	NM_001363223.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363223.1

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( NM_001363224.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350153.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 4, mRNA.

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CloneID	OMu78086
Clone ID Related Accession (Same CDS sequence)	XM_017314846.1 , NM_001363224.1
Accession Version	NM_001363224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL671968.17 and BX572640.9. On May 12, 2018 this sequence version replaced XM_017314846.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.120251.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350153.1
CDS	2178..4394
Translation	MLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	NM_001363224.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363224.1

ORF Insert Sequence:

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_030246433.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102293.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X17, mRNA.

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CloneID	OMu71775
Clone ID Related Accession (Same CDS sequence)	XM_030246433.1 , XM_011243794.2
Accession Version	XM_030246433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGTGCC TGGCCCTGAG GCTGGAAGAG CAGCAGAAAC TGAAGTGTGC GGCCCTGCAC 
AGCCAGTGCA TCCTGCTTCG CAGGGCTCTG CAGAAGTGGC TGGTTTACCA GAATAGGGTG 
AGGAGTGTTC TGCGGGAGGT GGCGGCCAGG GAGAGACAGC ACAACAGGCA GCTGCTGTGG 
TGGGCGTTAC ATCTCTGGAG AGAAAACACC ATGGCCCGTT TGGATGGAGC CAAAAAAACC 
TCTCAAGCAA GAGTTCACTA CAGCAGGACT CTGTGCTCCA AGGTCCTCGT CCAGTGGCGA 
GAGGTTACTT CAGTGCAGAT ATATTACCGA CAAAAGGAGG CAGCTGCCCT CAGGGAGGCC 
CGGAAAGCAC TGGACAGGGG TCGTCTCCAG AACTGGTTTC AGCACTGGCG ATTCTGCAGC 
CAGAGGGCAG CCCAGCAGAG ATTCCAGCTG GGACAGGCGG CTCAGCATCA CCACTGGCAG 
CTGCTGATGG AAGCAATGGC CAGGTGGAAG GCACACCATC TGGGGTGTAT CAGAAAGAAG 
TTTCTGCAGA GGCAAGCTGC CCAACTCCTG GCACAGAGAC TCAGTCGGGC CTGCTTCTGC 
CAGTGGAGGA AGCAGCTGGC AGTCCGGAAG CAAGAACAGT GGGGCACAGC CCGGGCCCTG 
TGGCTCTGGG CCTTCTCACT ACAAGCAAAG GTGTGGACTG CATGGCTGGG CTTCGTACTT 
GAGAGGAGGA GGAAGAAGGC ACGGCTGGAA CGGGCTATGC AAGCCTACCA GCAGCAGCTC 
CTACAGGAGG GTGCCACACG GCTTCTGCGC TTCACAGCTG GTACGAAGGC CTTCCGGCAG 
CAGCTCCAGG CCCAGCAGCA GGTCCAGGCA GCCCACAGCC TCCACTGTGC AGTCCGCCAC 
TGTGCTGAGC TCTGGAAAAA GAAGGTGCTT GGTCCAGGCA AGACTTCTCA ACCACCAGCA 
CCCACCACAT TCAGCAAGAG AGTGACATTT AAGGATTCTT TCCTCTCTGG ACATGCTGCG 
GAGGCTGGAG ATGCTACCCA GGAGACAAAG AAGCTTCGAG CTCCTCCATC TCAGGGAGTC 
CTGGGTAGTT TGGCAGGAGC TGCTGGGGAG CCCTGCCACC TAGACCTCAA TGCAGCCCGC 
TCATCACGGA AACAGCCCCG ACGTCCATCC TTCCTGCTTG AGCGTTTGGG GAGTCAGAGG 
TCCCCAGAGT GGTACAGCCT TGGGGAGCAG CAGTTAGAGA AGCCCCCAGA GGAGGAAAGC 
ACGGCTCTGC TGGGAGGCTC TTCCCTGACA AGACCCTTCC TACCTGGGGT CCTGCCGAAT 
GTTCCTGGTC CGAAGCTGCC TCCTACGGCC AGTCCAGGCC TGGAGCTGCT GCCTCCCTCC 
TCCATCATGC CCCATGCGGC AGGAGGCACT GCTAGGGTGT CAGCGAAGCC CAGCATCCCT 
GGGCCTCAGC CTTGGGGGTG CCCGTCCTTA CCCAGGGACC TTGATCCCCA ACTCCTCCCT 
GGAGATTCCA TAAGCACCCG GACTGAGCCT GTTTATGGCT CAGAGGCTAC AGGCCACACA 
GAGCTTGAGG CTGAGTTGGA GGGGATCCAG CAGCAGTTAC AACACTACCA AACCACCAAG 
CAGAACCTTT GGTCATGCCA GCGACAAGCC AACAGCCTGC GTAGGTGGCT GGAGCTAAGC 
CAGGAGGAGC CGAAATCCGA GGACCTTCAC CTAGAAGAGC AGGTGAAAAC AGAACTGGAG 
GAGGTAAGAC CAGGTCAGCC CAGGGCCAGC CCCTGGCTGT CCTTCCTTTC AGCTTGCCTT 
GTCCCTCCCT CTCGCCCATG TCCCCAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102293.1
CDS	1433..3904
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_030246433.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246433.1

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGTGCC TGGCCCTGAG GCTGGAAGAG CAGCAGAAAC TGAAGTGTGC GGCCCTGCAC 
AGCCAGTGCA TCCTGCTTCG CAGGGCTCTG CAGAAGTGGC TGGTTTACCA GAATAGGGTG 
AGGAGTGTTC TGCGGGAGGT GGCGGCCAGG GAGAGACAGC ACAACAGGCA GCTGCTGTGG 
TGGGCGTTAC ATCTCTGGAG AGAAAACACC ATGGCCCGTT TGGATGGAGC CAAAAAAACC 
TCTCAAGCAA GAGTTCACTA CAGCAGGACT CTGTGCTCCA AGGTCCTCGT CCAGTGGCGA 
GAGGTTACTT CAGTGCAGAT ATATTACCGA CAAAAGGAGG CAGCTGCCCT CAGGGAGGCC 
CGGAAAGCAC TGGACAGGGG TCGTCTCCAG AACTGGTTTC AGCACTGGCG ATTCTGCAGC 
CAGAGGGCAG CCCAGCAGAG ATTCCAGCTG GGACAGGCGG CTCAGCATCA CCACTGGCAG 
CTGCTGATGG AAGCAATGGC CAGGTGGAAG GCACACCATC TGGGGTGTAT CAGAAAGAAG 
TTTCTGCAGA GGCAAGCTGC CCAACTCCTG GCACAGAGAC TCAGTCGGGC CTGCTTCTGC 
CAGTGGAGGA AGCAGCTGGC AGTCCGGAAG CAAGAACAGT GGGGCACAGC CCGGGCCCTG 
TGGCTCTGGG CCTTCTCACT ACAAGCAAAG GTGTGGACTG CATGGCTGGG CTTCGTACTT 
GAGAGGAGGA GGAAGAAGGC ACGGCTGGAA CGGGCTATGC AAGCCTACCA GCAGCAGCTC 
CTACAGGAGG GTGCCACACG GCTTCTGCGC TTCACAGCTG GTACGAAGGC CTTCCGGCAG 
CAGCTCCAGG CCCAGCAGCA GGTCCAGGCA GCCCACAGCC TCCACTGTGC AGTCCGCCAC 
TGTGCTGAGC TCTGGAAAAA GAAGGTGCTT GGTCCAGGCA AGACTTCTCA ACCACCAGCA 
CCCACCACAT TCAGCAAGAG AGTGACATTT AAGGATTCTT TCCTCTCTGG ACATGCTGCG 
GAGGCTGGAG ATGCTACCCA GGAGACAAAG AAGCTTCGAG CTCCTCCATC TCAGGGAGTC 
CTGGGTAGTT TGGCAGGAGC TGCTGGGGAG CCCTGCCACC TAGACCTCAA TGCAGCCCGC 
TCATCACGGA AACAGCCCCG ACGTCCATCC TTCCTGCTTG AGCGTTTGGG GAGTCAGAGG 
TCCCCAGAGT GGTACAGCCT TGGGGAGCAG CAGTTAGAGA AGCCCCCAGA GGAGGAAAGC 
ACGGCTCTGC TGGGAGGCTC TTCCCTGACA AGACCCTTCC TACCTGGGGT CCTGCCGAAT 
GTTCCTGGTC CGAAGCTGCC TCCTACGGCC AGTCCAGGCC TGGAGCTGCT GCCTCCCTCC 
TCCATCATGC CCCATGCGGC AGGAGGCACT GCTAGGGTGT CAGCGAAGCC CAGCATCCCT 
GGGCCTCAGC CTTGGGGGTG CCCGTCCTTA CCCAGGGACC TTGATCCCCA ACTCCTCCCT 
GGAGATTCCA TAAGCACCCG GACTGAGCCT GTTTATGGCT CAGAGGCTAC AGGCCACACA 
GAGCTTGAGG CTGAGTTGGA GGGGATCCAG CAGCAGTTAC AACACTACCA AACCACCAAG 
CAGAACCTTT GGTCATGCCA GCGACAAGCC AACAGCCTGC GTAGGTGGCT GGAGCTAAGC 
CAGGAGGAGC CGAAATCCGA GGACCTTCAC CTAGAAGAGC AGGTGAAAAC AGAACTGGAG 
GAGGTAAGAC CAGGTCAGCC CAGGGCCAGC CCCTGGCTGT CCTTCCTTTC AGCTTGCCTT 
GTCCCTCCCT CTCGCCCATG TCCCCAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_030246434.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102294.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X21, mRNA.

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CloneID	OMu71777
Clone ID Related Accession (Same CDS sequence)	XM_030246434.1 , XM_017314844.1
Accession Version	XM_030246434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTAAGACC AGGTCAGCCC AGGGCCAGCC CCTGGCTGTC CTTCCTTTCA 
GCTTGCCTTG TCCCTCCCTC TCGCCCATGT CCCCAGGTGG AACTACAGGT CCAGCAGCTG 
GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT GTTGGTACAT GCATTGCTCG TGTTCGGGCC 
CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102294.1
CDS	1441..3741
Translation	MLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_030246434.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246434.1

ORF Insert Sequence:

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTAAGACC AGGTCAGCCC AGGGCCAGCC CCTGGCTGTC CTTCCTTTCA 
GCTTGCCTTG TCCCTCCCTC TCGCCCATGT CCCCAGGTGG AACTACAGGT CCAGCAGCTG 
GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT GTTGGTACAT GCATTGCTCG TGTTCGGGCC 
CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_011243797.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242099.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X23, mRNA.

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CloneID	OMu71778
Clone ID Related Accession (Same CDS sequence)	XM_011243798.1 , XM_011243797.2 , XM_011243797.3
Accession Version	XM_011243797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011243797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242099.1
CDS	2327..4615
Translation	MCTRDNGSRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_011243797.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011243797.3

ORF Insert Sequence:

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314845.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170334.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OMu78085
Clone ID Related Accession (Same CDS sequence)	XM_017314845.1 , NM_001363223.1
Accession Version	XM_017314845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170334.1
CDS	2170..4461
Translation	MAEWVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314845.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314845.1

ORF Insert Sequence:

ATGGCAGAAT GGGTGACAGT CCTACACAAG TGTGTCAGGG TGTGGCTACG GTATGTGCAC 
AAGAGACAAT GGCAGCAGTT ATTGCGGGCC AGAGCAGATG GTCATTTCCA GCAGCGAGCA 
CTGCCTGCTG CCTTCTACAC GTGGTACAGA GGCTGGCTAT GGCACCAGCA GAGACGGATC 
CTGCACACAA AAGCAGTGCG TTTCCACAGA GGGACATTAG AGAAGCAAGT ATTTGCTCTC 
TGGAGGCAGA AGATGTCTCA GCATCGAGAA AACTGCTTGG CAGAGAGAAT GGCTATACTG 
CAGGCAGAGC AGCAGCTTTT GCGTAGGTTC TGGTTCGTGT GGCACCAGCA GGCAGCAGTG 
TGTCAGCTGG AGCGACAGCA GCAAGCCATG GCCATTGCCC ACCACCACAG TGGACTGCTC 
AGGAGGGCTT TCTGCATCTG GAAGGAAAGC ACCCAAGGAT TTAGGATAGA GAGGATGGGC 
AGGGCACAGG CTGCGCACTT CCACTCTGCA CAGCTCTTGA GTCGGGCCTG GAGCATGTGG 
AGGGAGGTTT ACCAGAATAG GGTGAGGAGT GTTCTGCGGG AGGTGGCGGC CAGGGAGAGA 
CAGCACAACA GGCAGCTGCT GTGGTGGGCG TTACATCTCT GGAGAGAAAA CACCATGGCC 
CGTTTGGATG GAGCCAAAAA AACCTCTCAA GCAAGAGTTC ACTACAGCAG GACTCTGTGC 
TCCAAGGTCC TCGTCCAGTG GCGAGAGGTT ACTTCAGTGC AGATATATTA CCGACAAAAG 
GAGGCAGCTG CCCTCAGGGA GGCCCGGAAA GCACTGGACA GGGGTCGTCT CCAGAACTGG 
TTTCAGCACT GGCGATTCTG CAGCCAGAGG GCAGCCCAGC AGAGATTCCA GCTGGGACAG 
GCGGCTCAGC ATCACCACTG GCAGCTGCTG ATGGAAGCAA TGGCCAGGTG GAAGGCACAC 
CATCTGGGGT GTATCAGAAA GAAGTTTCTG CAGAGGCAAG CTGCCCAACT CCTGGCACAG 
AGACTCAGTC GGGCCTGCTT CTGCCAGTGG AGGAAGCAGC TGGCAGTCCG GAAGCAAGAA 
CAGTGGGGCA CAGCCCGGGC CCTGTGGCTC TGGGCCTTCT CACTACAAGC AAAGGTGTGG 
ACTGCATGGC TGGGCTTCGT ACTTGAGAGG AGGAGGAAGA AGGCACGGCT GGAACGGGCT 
ATGCAAGCCT ACCAGCAGCA GCTCCTACAG GAGGGTGCCA CACGGCTTCT GCGCTTCACA 
GCTGGTACGA AGGCCTTCCG GCAGCAGCTC CAGGCCCAGC AGCAGGTCCA GGCAGCCCAC 
AGCCTCCACT GTGCAGTCCG CCACTGTGCT GAGCTCTGGA AAAAGAAGGT GCTTGGTCCA 
GGCAAGACTT CTCAACCACC AGCACCCACC ACATTCAGCA AGAGAGTGAC ATTTAAGGAT 
TCTTTCCTCT CTGGACATGC TGCGGAGGCT GGAGATGCTA CCCAGGAGAC AAAGAAGCTT 
CGAGCTCCTC CATCTCAGGG AGTCCTGGGT AGTTTGGCAG GAGCTGCTGG GGAGCCCTGC 
CACCTAGACC TCAATGCAGC CCGCTCATCA CGGAAACAGC CCCGACGTCC ATCCTTCCTG 
CTTGAGCGTT TGGGGAGTCA GAGGTCCCCA GAGTGGTACA GCCTTGGGGA GCAGCAGTTA 
GAGAAGCCCC CAGAGGAGGA AAGCACGGCT CTGCTGGGAG GCTCTTCCCT GACAAGACCC 
TTCCTACCTG GGGTCCTGCC GAATGTTCCT GGTCCGAAGC TGCCTCCTAC GGCCAGTCCA 
GGCCTGGAGC TGCTGCCTCC CTCCTCCATC ATGCCCCATG CGGCAGGAGG CACTGCTAGG 
GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT CAGCCTTGGG GGTGCCCGTC CTTACCCAGG 
GACCTTGATC CCCAACTCCT CCCTGGAGAT TCCATAAGCA CCCGGACTGA GCCTGTTTAT 
GGCTCAGAGG CTACAGGCCA CACAGAGCTT GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG 
TTACAACACT ACCAAACCAC CAAGCAGAAC CTTTGGTCAT GCCAGCGACA AGCCAACAGC 
CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG GAGCCGAAAT CCGAGGACCT TCACCTAGAA 
GAGCAGGTGA AAACAGAACT GGAGGAGGTG GAACTACAGG TCCAGCAGCT GGCTAAGGAG 
CTTGAGGCCC AGCGTCAGCC TGTTGGTACA TGCATTGCTC GTGTTCGGGC CCTGAGACGG 
GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314846.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170335.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OMu78086
Clone ID Related Accession (Same CDS sequence)	XM_017314846.1 , NM_001363224.1
Accession Version	XM_017314846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170335.1
CDS	2274..4490
Translation	MLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314846.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314846.1

ORF Insert Sequence:

ATGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GGTTTACCAG 
AATAGGGTGA GGAGTGTTCT GCGGGAGGTG GCGGCCAGGG AGAGACAGCA CAACAGGCAG 
CTGCTGTGGT GGGCGTTACA TCTCTGGAGA GAAAACACCA TGGCCCGTTT GGATGGAGCC 
AAAAAAACCT CTCAAGCAAG AGTTCACTAC AGCAGGACTC TGTGCTCCAA GGTCCTCGTC 
CAGTGGCGAG AGGTTACTTC AGTGCAGATA TATTACCGAC AAAAGGAGGC AGCTGCCCTC 
AGGGAGGCCC GGAAAGCACT GGACAGGGGT CGTCTCCAGA ACTGGTTTCA GCACTGGCGA 
TTCTGCAGCC AGAGGGCAGC CCAGCAGAGA TTCCAGCTGG GACAGGCGGC TCAGCATCAC 
CACTGGCAGC TGCTGATGGA AGCAATGGCC AGGTGGAAGG CACACCATCT GGGGTGTATC 
AGAAAGAAGT TTCTGCAGAG GCAAGCTGCC CAACTCCTGG CACAGAGACT CAGTCGGGCC 
TGCTTCTGCC AGTGGAGGAA GCAGCTGGCA GTCCGGAAGC AAGAACAGTG GGGCACAGCC 
CGGGCCCTGT GGCTCTGGGC CTTCTCACTA CAAGCAAAGG TGTGGACTGC ATGGCTGGGC 
TTCGTACTTG AGAGGAGGAG GAAGAAGGCA CGGCTGGAAC GGGCTATGCA AGCCTACCAG 
CAGCAGCTCC TACAGGAGGG TGCCACACGG CTTCTGCGCT TCACAGCTGG TACGAAGGCC 
TTCCGGCAGC AGCTCCAGGC CCAGCAGCAG GTCCAGGCAG CCCACAGCCT CCACTGTGCA 
GTCCGCCACT GTGCTGAGCT CTGGAAAAAG AAGGTGCTTG GTCCAGGCAA GACTTCTCAA 
CCACCAGCAC CCACCACATT CAGCAAGAGA GTGACATTTA AGGATTCTTT CCTCTCTGGA 
CATGCTGCGG AGGCTGGAGA TGCTACCCAG GAGACAAAGA AGCTTCGAGC TCCTCCATCT 
CAGGGAGTCC TGGGTAGTTT GGCAGGAGCT GCTGGGGAGC CCTGCCACCT AGACCTCAAT 
GCAGCCCGCT CATCACGGAA ACAGCCCCGA CGTCCATCCT TCCTGCTTGA GCGTTTGGGG 
AGTCAGAGGT CCCCAGAGTG GTACAGCCTT GGGGAGCAGC AGTTAGAGAA GCCCCCAGAG 
GAGGAAAGCA CGGCTCTGCT GGGAGGCTCT TCCCTGACAA GACCCTTCCT ACCTGGGGTC 
CTGCCGAATG TTCCTGGTCC GAAGCTGCCT CCTACGGCCA GTCCAGGCCT GGAGCTGCTG 
CCTCCCTCCT CCATCATGCC CCATGCGGCA GGAGGCACTG CTAGGGTGTC AGCGAAGCCC 
AGCATCCCTG GGCCTCAGCC TTGGGGGTGC CCGTCCTTAC CCAGGGACCT TGATCCCCAA 
CTCCTCCCTG GAGATTCCAT AAGCACCCGG ACTGAGCCTG TTTATGGCTC AGAGGCTACA 
GGCCACACAG AGCTTGAGGC TGAGTTGGAG GGGATCCAGC AGCAGTTACA ACACTACCAA 
ACCACCAAGC AGAACCTTTG GTCATGCCAG CGACAAGCCA ACAGCCTGCG TAGGTGGCTG 
GAGCTAAGCC AGGAGGAGCC GAAATCCGAG GACCTTCACC TAGAAGAGCA GGTGAAAACA 
GAACTGGAGG AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314847.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170336.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OMu78087
Clone ID Related Accession (Same CDS sequence)	XM_017314847.1 , XM_017314847.2
Accession Version	XM_017314847.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGGTTTACC AGAATAGGGT GAGGAGTGTT CTGCGGGAGG TGGCGGCCAG GGAGAGACAG 
CACAACAGGC AGCTGCTGTG GTGGGCGTTA CATCTCTGGA GAGAAAACAC CATGGCCCGT 
TTGGATGGAG CCAAAAAAAC CTCTCAAGCA AGAGTTCACT ACAGCAGGAC TCTGTGCTCC 
AAGGTCCTCG TCCAGTGGCG AGAGGTTACT TCAGTGCAGA TATATTACCG ACAAAAGGAG 
GCAGCTGCCC TCAGGGAGGC CCGGAAAGCA CTGGACAGGG GTCGTCTCCA GAACTGGTTT 
CAGCACTGGC GATTCTGCAG CCAGAGGGCA GCCCAGCAGA GATTCCAGCT GGGACAGGCG 
GCTCAGCATC ACCACTGGCA GCTGCTGATG GAAGCAATGG CCAGGTGGAA GGCACACCAT 
CTGGGGTGTA TCAGAAAGAA GTTTCTGCAG AGGCAAGCTG CCCAACTCCT GGCACAGAGA 
CTCAGTCGGG CCTGCTTCTG CCAGTGGAGG AAGCAGCTGG CAGTCCGGAA GCAAGAACAG 
TGGGGCACAG CCCGGGCCCT GTGGCTCTGG GCCTTCTCAC TACAAGCAAA GGTGTGGACT 
GCATGGCTGG GCTTCGTACT TGAGAGGAGG AGGAAGAAGG CACGGCTGGA ACGGGCTATG 
CAAGCCTACC AGCAGCAGCT CCTACAGGAG GGTGCCACAC GGCTTCTGCG CTTCACAGCT 
GGTACGAAGG CCTTCCGGCA GCAGCTCCAG GCCCAGCAGC AGGTCCAGGC AGCCCACAGC 
CTCCACTGTG CAGTCCGCCA CTGTGCTGAG CTCTGGAAAA AGAAGGTGCT TGGTCCAGGC 
AAGACTTCTC AACCACCAGC ACCCACCACA TTCAGCAAGA GAGTGACATT TAAGGATTCT 
TTCCTCTCTG GACATGCTGC GGAGGCTGGA GATGCTACCC AGGAGACAAA GAAGCTTCGA 
GCTCCTCCAT CTCAGGGAGT CCTGGGTAGT TTGGCAGGAG CTGCTGGGGA GCCCTGCCAC 
CTAGACCTCA ATGCAGCCCG CTCATCACGG AAACAGCCCC GACGTCCATC CTTCCTGCTT 
GAGCGTTTGG GGAGTCAGAG GTCCCCAGAG TGGTACAGCC TTGGGGAGCA GCAGTTAGAG 
AAGCCCCCAG AGGAGGAAAG CACGGCTCTG CTGGGAGGCT CTTCCCTGAC AAGACCCTTC 
CTACCTGGGG TCCTGCCGAA TGTTCCTGGT CCGAAGCTGC CTCCTACGGC CAGTCCAGGC 
CTGGAGCTGC TGCCTCCCTC CTCCATCATG CCCCATGCGG CAGGAGGCAC TGCTAGGGTG 
TCAGCGAAGC CCAGCATCCC TGGGCCTCAG CCTTGGGGGT GCCCGTCCTT ACCCAGGGAC 
CTTGATCCCC AACTCCTCCC TGGAGATTCC ATAAGCACCC GGACTGAGCC TGTTTATGGC 
TCAGAGGCTA CAGGCCACAC AGAGCTTGAG GCTGAGTTGG AGGGGATCCA GCAGCAGTTA 
CAACACTACC AAACCACCAA GCAGAACCTT TGGTCATGCC AGCGACAAGC CAACAGCCTG 
CGTAGGTGGC TGGAGCTAAG CCAGGAGGAG CCGAAATCCG AGGACCTTCA CCTAGAAGAG 
CAGGTGAAAA CAGAACTGGA GGAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170336.1
CDS	2323..4431
Translation	MCTRDNGSRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314847.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314847.1

ORF Insert Sequence:

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGGTTTACC AGAATAGGGT GAGGAGTGTT CTGCGGGAGG TGGCGGCCAG GGAGAGACAG 
CACAACAGGC AGCTGCTGTG GTGGGCGTTA CATCTCTGGA GAGAAAACAC CATGGCCCGT 
TTGGATGGAG CCAAAAAAAC CTCTCAAGCA AGAGTTCACT ACAGCAGGAC TCTGTGCTCC 
AAGGTCCTCG TCCAGTGGCG AGAGGTTACT TCAGTGCAGA TATATTACCG ACAAAAGGAG 
GCAGCTGCCC TCAGGGAGGC CCGGAAAGCA CTGGACAGGG GTCGTCTCCA GAACTGGTTT 
CAGCACTGGC GATTCTGCAG CCAGAGGGCA GCCCAGCAGA GATTCCAGCT GGGACAGGCG 
GCTCAGCATC ACCACTGGCA GCTGCTGATG GAAGCAATGG CCAGGTGGAA GGCACACCAT 
CTGGGGTGTA TCAGAAAGAA GTTTCTGCAG AGGCAAGCTG CCCAACTCCT GGCACAGAGA 
CTCAGTCGGG CCTGCTTCTG CCAGTGGAGG AAGCAGCTGG CAGTCCGGAA GCAAGAACAG 
TGGGGCACAG CCCGGGCCCT GTGGCTCTGG GCCTTCTCAC TACAAGCAAA GGTGTGGACT 
GCATGGCTGG GCTTCGTACT TGAGAGGAGG AGGAAGAAGG CACGGCTGGA ACGGGCTATG 
CAAGCCTACC AGCAGCAGCT CCTACAGGAG GGTGCCACAC GGCTTCTGCG CTTCACAGCT 
GGTACGAAGG CCTTCCGGCA GCAGCTCCAG GCCCAGCAGC AGGTCCAGGC AGCCCACAGC 
CTCCACTGTG CAGTCCGCCA CTGTGCTGAG CTCTGGAAAA AGAAGGTGCT TGGTCCAGGC 
AAGACTTCTC AACCACCAGC ACCCACCACA TTCAGCAAGA GAGTGACATT TAAGGATTCT 
TTCCTCTCTG GACATGCTGC GGAGGCTGGA GATGCTACCC AGGAGACAAA GAAGCTTCGA 
GCTCCTCCAT CTCAGGGAGT CCTGGGTAGT TTGGCAGGAG CTGCTGGGGA GCCCTGCCAC 
CTAGACCTCA ATGCAGCCCG CTCATCACGG AAACAGCCCC GACGTCCATC CTTCCTGCTT 
GAGCGTTTGG GGAGTCAGAG GTCCCCAGAG TGGTACAGCC TTGGGGAGCA GCAGTTAGAG 
AAGCCCCCAG AGGAGGAAAG CACGGCTCTG CTGGGAGGCT CTTCCCTGAC AAGACCCTTC 
CTACCTGGGG TCCTGCCGAA TGTTCCTGGT CCGAAGCTGC CTCCTACGGC CAGTCCAGGC 
CTGGAGCTGC TGCCTCCCTC CTCCATCATG CCCCATGCGG CAGGAGGCAC TGCTAGGGTG 
TCAGCGAAGC CCAGCATCCC TGGGCCTCAG CCTTGGGGGT GCCCGTCCTT ACCCAGGGAC 
CTTGATCCCC AACTCCTCCC TGGAGATTCC ATAAGCACCC GGACTGAGCC TGTTTATGGC 
TCAGAGGCTA CAGGCCACAC AGAGCTTGAG GCTGAGTTGG AGGGGATCCA GCAGCAGTTA 
CAACACTACC AAACCACCAA GCAGAACCTT TGGTCATGCC AGCGACAAGC CAACAGCCTG 
CGTAGGTGGC TGGAGCTAAG CCAGGAGGAG CCGAAATCCG AGGACCTTCA CCTAGAAGAG 
CAGGTGAAAA CAGAACTGGA GGAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314847.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170336.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu78087
Clone ID Related Accession (Same CDS sequence)	XM_017314847.1 , XM_017314847.2
Accession Version	XM_017314847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGGTTTACC AGAATAGGGT GAGGAGTGTT CTGCGGGAGG TGGCGGCCAG GGAGAGACAG 
CACAACAGGC AGCTGCTGTG GTGGGCGTTA CATCTCTGGA GAGAAAACAC CATGGCCCGT 
TTGGATGGAG CCAAAAAAAC CTCTCAAGCA AGAGTTCACT ACAGCAGGAC TCTGTGCTCC 
AAGGTCCTCG TCCAGTGGCG AGAGGTTACT TCAGTGCAGA TATATTACCG ACAAAAGGAG 
GCAGCTGCCC TCAGGGAGGC CCGGAAAGCA CTGGACAGGG GTCGTCTCCA GAACTGGTTT 
CAGCACTGGC GATTCTGCAG CCAGAGGGCA GCCCAGCAGA GATTCCAGCT GGGACAGGCG 
GCTCAGCATC ACCACTGGCA GCTGCTGATG GAAGCAATGG CCAGGTGGAA GGCACACCAT 
CTGGGGTGTA TCAGAAAGAA GTTTCTGCAG AGGCAAGCTG CCCAACTCCT GGCACAGAGA 
CTCAGTCGGG CCTGCTTCTG CCAGTGGAGG AAGCAGCTGG CAGTCCGGAA GCAAGAACAG 
TGGGGCACAG CCCGGGCCCT GTGGCTCTGG GCCTTCTCAC TACAAGCAAA GGTGTGGACT 
GCATGGCTGG GCTTCGTACT TGAGAGGAGG AGGAAGAAGG CACGGCTGGA ACGGGCTATG 
CAAGCCTACC AGCAGCAGCT CCTACAGGAG GGTGCCACAC GGCTTCTGCG CTTCACAGCT 
GGTACGAAGG CCTTCCGGCA GCAGCTCCAG GCCCAGCAGC AGGTCCAGGC AGCCCACAGC 
CTCCACTGTG CAGTCCGCCA CTGTGCTGAG CTCTGGAAAA AGAAGGTGCT TGGTCCAGGC 
AAGACTTCTC AACCACCAGC ACCCACCACA TTCAGCAAGA GAGTGACATT TAAGGATTCT 
TTCCTCTCTG GACATGCTGC GGAGGCTGGA GATGCTACCC AGGAGACAAA GAAGCTTCGA 
GCTCCTCCAT CTCAGGGAGT CCTGGGTAGT TTGGCAGGAG CTGCTGGGGA GCCCTGCCAC 
CTAGACCTCA ATGCAGCCCG CTCATCACGG AAACAGCCCC GACGTCCATC CTTCCTGCTT 
GAGCGTTTGG GGAGTCAGAG GTCCCCAGAG TGGTACAGCC TTGGGGAGCA GCAGTTAGAG 
AAGCCCCCAG AGGAGGAAAG CACGGCTCTG CTGGGAGGCT CTTCCCTGAC AAGACCCTTC 
CTACCTGGGG TCCTGCCGAA TGTTCCTGGT CCGAAGCTGC CTCCTACGGC CAGTCCAGGC 
CTGGAGCTGC TGCCTCCCTC CTCCATCATG CCCCATGCGG CAGGAGGCAC TGCTAGGGTG 
TCAGCGAAGC CCAGCATCCC TGGGCCTCAG CCTTGGGGGT GCCCGTCCTT ACCCAGGGAC 
CTTGATCCCC AACTCCTCCC TGGAGATTCC ATAAGCACCC GGACTGAGCC TGTTTATGGC 
TCAGAGGCTA CAGGCCACAC AGAGCTTGAG GCTGAGTTGG AGGGGATCCA GCAGCAGTTA 
CAACACTACC AAACCACCAA GCAGAACCTT TGGTCATGCC AGCGACAAGC CAACAGCCTG 
CGTAGGTGGC TGGAGCTAAG CCAGGAGGAG CCGAAATCCG AGGACCTTCA CCTAGAAGAG 
CAGGTGAAAA CAGAACTGGA GGAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170336.1
CDS	2327..4435
Translation	MCTRDNGSRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314847.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314847.2

ORF Insert Sequence:

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGGTTTACC AGAATAGGGT GAGGAGTGTT CTGCGGGAGG TGGCGGCCAG GGAGAGACAG 
CACAACAGGC AGCTGCTGTG GTGGGCGTTA CATCTCTGGA GAGAAAACAC CATGGCCCGT 
TTGGATGGAG CCAAAAAAAC CTCTCAAGCA AGAGTTCACT ACAGCAGGAC TCTGTGCTCC 
AAGGTCCTCG TCCAGTGGCG AGAGGTTACT TCAGTGCAGA TATATTACCG ACAAAAGGAG 
GCAGCTGCCC TCAGGGAGGC CCGGAAAGCA CTGGACAGGG GTCGTCTCCA GAACTGGTTT 
CAGCACTGGC GATTCTGCAG CCAGAGGGCA GCCCAGCAGA GATTCCAGCT GGGACAGGCG 
GCTCAGCATC ACCACTGGCA GCTGCTGATG GAAGCAATGG CCAGGTGGAA GGCACACCAT 
CTGGGGTGTA TCAGAAAGAA GTTTCTGCAG AGGCAAGCTG CCCAACTCCT GGCACAGAGA 
CTCAGTCGGG CCTGCTTCTG CCAGTGGAGG AAGCAGCTGG CAGTCCGGAA GCAAGAACAG 
TGGGGCACAG CCCGGGCCCT GTGGCTCTGG GCCTTCTCAC TACAAGCAAA GGTGTGGACT 
GCATGGCTGG GCTTCGTACT TGAGAGGAGG AGGAAGAAGG CACGGCTGGA ACGGGCTATG 
CAAGCCTACC AGCAGCAGCT CCTACAGGAG GGTGCCACAC GGCTTCTGCG CTTCACAGCT 
GGTACGAAGG CCTTCCGGCA GCAGCTCCAG GCCCAGCAGC AGGTCCAGGC AGCCCACAGC 
CTCCACTGTG CAGTCCGCCA CTGTGCTGAG CTCTGGAAAA AGAAGGTGCT TGGTCCAGGC 
AAGACTTCTC AACCACCAGC ACCCACCACA TTCAGCAAGA GAGTGACATT TAAGGATTCT 
TTCCTCTCTG GACATGCTGC GGAGGCTGGA GATGCTACCC AGGAGACAAA GAAGCTTCGA 
GCTCCTCCAT CTCAGGGAGT CCTGGGTAGT TTGGCAGGAG CTGCTGGGGA GCCCTGCCAC 
CTAGACCTCA ATGCAGCCCG CTCATCACGG AAACAGCCCC GACGTCCATC CTTCCTGCTT 
GAGCGTTTGG GGAGTCAGAG GTCCCCAGAG TGGTACAGCC TTGGGGAGCA GCAGTTAGAG 
AAGCCCCCAG AGGAGGAAAG CACGGCTCTG CTGGGAGGCT CTTCCCTGAC AAGACCCTTC 
CTACCTGGGG TCCTGCCGAA TGTTCCTGGT CCGAAGCTGC CTCCTACGGC CAGTCCAGGC 
CTGGAGCTGC TGCCTCCCTC CTCCATCATG CCCCATGCGG CAGGAGGCAC TGCTAGGGTG 
TCAGCGAAGC CCAGCATCCC TGGGCCTCAG CCTTGGGGGT GCCCGTCCTT ACCCAGGGAC 
CTTGATCCCC AACTCCTCCC TGGAGATTCC ATAAGCACCC GGACTGAGCC TGTTTATGGC 
TCAGAGGCTA CAGGCCACAC AGAGCTTGAG GCTGAGTTGG AGGGGATCCA GCAGCAGTTA 
CAACACTACC AAACCACCAA GCAGAACCTT TGGTCATGCC AGCGACAAGC CAACAGCCTG 
CGTAGGTGGC TGGAGCTAAG CCAGGAGGAG CCGAAATCCG AGGACCTTCA CCTAGAAGAG 
CAGGTGAAAA CAGAACTGGA GGAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_030246435.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102295.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OMu85949
Clone ID Related Accession (Same CDS sequence)	XM_030246435.1
Accession Version	XM_030246435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGGTTTACC AGAATAGGGT GAGGAGTGTT CTGCGGGAGG TGGCGGCCAG GGAGAGACAG 
CACAACAGGC AGCTGCTGTG GTGGGCGTTA CATCTCTGGA GAGAAAACAC CATGGCCCGT 
TTGGATGGAG CCAAAAAAAC CTCTCAAGCA AGAGTTCACT ACAGCAGGAC TCTGTGCTCC 
AAGGTCCTCG TCCAGTGGCG AGAGGTTACT TCAGTGCAGA TATATTACCG ACAAAAGGAG 
GCAGCTGCCC TCAGGGAGGC CCGGAAAGCA CTGGACAGGG GTCGTCTCCA GAACTGGTTT 
CAGCACTGGC GATTCTGCAG CCAGAGGGCA GCCCAGCAGA GATTCCAGCT GGGACAGGCG 
GCTCAGCATC ACCACTGGCA GCTGCTGATG GAAGCAATGG CCAGGTGGAA GGCACACCAT 
CTGGGGTGTA TCAGAAAGAA GTTTCTGCAG AGGCAAGCTG CCCAACTCCT GGCACAGAGA 
CTCAGTCGGG CCTGCTTCTG CCAGTGGAGG AAGCAGCTGG CAGTCCGGAA GCAAGAACAG 
TGGGGCACAG CCCGGGCCCT GTGGCTCTGG GCCTTCTCAC TACAAGCAAA GGTGTGGACT 
GCATGGCTGG GCTTCGTACT TGAGAGGAGG AGGAAGAAGG CACGGCTGGA ACGGGCTATG 
CAAGCCTACC AGCAGCAGCT CCTACAGGAG GGTGCCACAC GGCTTCTGCG CTTCACAGCT 
GGTACGAAGG CCTTCCGGCA GCAGCTCCAG GCCCAGCAGC AGGTCCAGGC AGCCCACAGC 
CTCCACTGTG CAGTCCGCCA CTGTGCTGAG CTCTGGAAAA AGAAGGTGCT TGGTCCAGGC 
AAGACTTCTC AACCACCAGC ACCCACCACA TTCAGCAAGA GAGTGACATT TAAGGATTCT 
TTCCTCTCTG GACATGCTGC GGAGGCTGGA GATGCTACCC AGGAGACAAA GAAGCTTCGA 
GCTCCTCCAT CTCAGGGAGT CCTGGGTAGT TTGGCAGGAG CTGCTGGGGA GCCCTGCCAC 
CTAGACCTCA ATGCAGCCCG CTCATCACGG AAACAGCCCC GACGTCCATC CTTCCTGCTT 
GAGCGTTTGG GGAGTCAGAG GTCCCCAGAG TGGTACAGCC TTGGGGAGCA GCAGTTAGAG 
AAGCCCCCAG AGGAGGAAAG CACGGCTCTG CTGGGAGGCT CTTCCCTGAC AAGACCCTTC 
CTACCTGGGG TCCTGCCGAA TGTTCCTGGT CCGAAGCTGC CTCCTACGGC CAGTCCAGGC 
CTGGAGCTGC TGCCTCCCTC CTCCATCATG CCCCATGCGG CAGGAGGCAC TGCTAGGGTG 
TCAGCGAAGC CCAGCATCCC TGGGCCTCAG CCTTGGGGGT GCCCGTCCTT ACCCAGGGAC 
CTTGATCCCC AACTCCTCCC TGGAGATTCC ATAAGCACCC GGACTGAGCC TGTTTATGGC 
TCAGAGGCTA CAGGCCACAC AGAGCTTGAG GCTGAGTTGG AGGGGATCCA GCAGCAGTTA 
CAACACTACC AAACCACCAA GCAGAACCTT TGGTCATGCC AGCGACAAGC CAACAGCCTG 
CGTAGGTGGC TGGAGCTAAG CCAGGAGGAG CCGAAATCCG AGGACCTTCA CCTAGAAGAG 
CAGGTGAAAA CAGAACTGGA GGAGGTAAGA CCAGGTCAGC CCAGGGCCAG CCCCTGGCTG 
TCCTTCCTTT CAGCTTGCCT TGTCCCTCCC TCTCGCCCAT GTCCCCAGGT GGAACTACAG 
GTCCAGCAGC TGGCTAAGGA GCTTGAGGCC CAGCGTCAGC CTGTTGGTAC ATGCATTGCT 
CGTGTTCGGG CCCTGAGACG GGCACTGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102295.1
CDS	2794..4986
Translation	MCTRDNGSRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_030246435.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246435.1

ORF Insert Sequence:

ATGTGCACAA GAGACAATGG CAGCAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGGTTTACC AGAATAGGGT GAGGAGTGTT CTGCGGGAGG TGGCGGCCAG GGAGAGACAG 
CACAACAGGC AGCTGCTGTG GTGGGCGTTA CATCTCTGGA GAGAAAACAC CATGGCCCGT 
TTGGATGGAG CCAAAAAAAC CTCTCAAGCA AGAGTTCACT ACAGCAGGAC TCTGTGCTCC 
AAGGTCCTCG TCCAGTGGCG AGAGGTTACT TCAGTGCAGA TATATTACCG ACAAAAGGAG 
GCAGCTGCCC TCAGGGAGGC CCGGAAAGCA CTGGACAGGG GTCGTCTCCA GAACTGGTTT 
CAGCACTGGC GATTCTGCAG CCAGAGGGCA GCCCAGCAGA GATTCCAGCT GGGACAGGCG 
GCTCAGCATC ACCACTGGCA GCTGCTGATG GAAGCAATGG CCAGGTGGAA GGCACACCAT 
CTGGGGTGTA TCAGAAAGAA GTTTCTGCAG AGGCAAGCTG CCCAACTCCT GGCACAGAGA 
CTCAGTCGGG CCTGCTTCTG CCAGTGGAGG AAGCAGCTGG CAGTCCGGAA GCAAGAACAG 
TGGGGCACAG CCCGGGCCCT GTGGCTCTGG GCCTTCTCAC TACAAGCAAA GGTGTGGACT 
GCATGGCTGG GCTTCGTACT TGAGAGGAGG AGGAAGAAGG CACGGCTGGA ACGGGCTATG 
CAAGCCTACC AGCAGCAGCT CCTACAGGAG GGTGCCACAC GGCTTCTGCG CTTCACAGCT 
GGTACGAAGG CCTTCCGGCA GCAGCTCCAG GCCCAGCAGC AGGTCCAGGC AGCCCACAGC 
CTCCACTGTG CAGTCCGCCA CTGTGCTGAG CTCTGGAAAA AGAAGGTGCT TGGTCCAGGC 
AAGACTTCTC AACCACCAGC ACCCACCACA TTCAGCAAGA GAGTGACATT TAAGGATTCT 
TTCCTCTCTG GACATGCTGC GGAGGCTGGA GATGCTACCC AGGAGACAAA GAAGCTTCGA 
GCTCCTCCAT CTCAGGGAGT CCTGGGTAGT TTGGCAGGAG CTGCTGGGGA GCCCTGCCAC 
CTAGACCTCA ATGCAGCCCG CTCATCACGG AAACAGCCCC GACGTCCATC CTTCCTGCTT 
GAGCGTTTGG GGAGTCAGAG GTCCCCAGAG TGGTACAGCC TTGGGGAGCA GCAGTTAGAG 
AAGCCCCCAG AGGAGGAAAG CACGGCTCTG CTGGGAGGCT CTTCCCTGAC AAGACCCTTC 
CTACCTGGGG TCCTGCCGAA TGTTCCTGGT CCGAAGCTGC CTCCTACGGC CAGTCCAGGC 
CTGGAGCTGC TGCCTCCCTC CTCCATCATG CCCCATGCGG CAGGAGGCAC TGCTAGGGTG 
TCAGCGAAGC CCAGCATCCC TGGGCCTCAG CCTTGGGGGT GCCCGTCCTT ACCCAGGGAC 
CTTGATCCCC AACTCCTCCC TGGAGATTCC ATAAGCACCC GGACTGAGCC TGTTTATGGC 
TCAGAGGCTA CAGGCCACAC AGAGCTTGAG GCTGAGTTGG AGGGGATCCA GCAGCAGTTA 
CAACACTACC AAACCACCAA GCAGAACCTT TGGTCATGCC AGCGACAAGC CAACAGCCTG 
CGTAGGTGGC TGGAGCTAAG CCAGGAGGAG CCGAAATCCG AGGACCTTCA CCTAGAAGAG 
CAGGTGAAAA CAGAACTGGA GGAGGTAAGA CCAGGTCAGC CCAGGGCCAG CCCCTGGCTG 
TCCTTCCTTT CAGCTTGCCT TGTCCCTCCC TCTCGCCCAT GTCCCCAGGT GGAACTACAG 
GTCCAGCAGC TGGCTAAGGA GCTTGAGGCC CAGCGTCAGC CTGTTGGTAC ATGCATTGCT 
CGTGTTCGGG CCCTGAGACG GGCACTGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314842.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170331.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X3, mRNA.

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CloneID	OMu45318
Clone ID Related Accession (Same CDS sequence)	XM_006514894.3 , XM_017314842.1 , XM_017314842.2 , XM_017314841.1
Accession Version	XM_017314842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170331.1
CDS	2391..6125
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314842.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314842.2

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514902.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514965.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu45323
Clone ID Related Accession (Same CDS sequence)	XM_006514902.4 , XM_006514902.3
Accession Version	XM_006514902.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514902.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTA 
AGACCAGGTC AGCCCAGGGC CAGCCCCTGG CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT 
CCCTCTCGCC CATGTCCCCA GGTGGAACTA CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG 
GCCCAGCGTC AGCCTGTTGG TACATGCATT GCTCGTGTTC GGGCCCTGAG ACGGGCACTG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514965.1
CDS	598..3783
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514902.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514902.4

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTA 
AGACCAGGTC AGCCCAGGGC CAGCCCCTGG CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT 
CCCTCTCGCC CATGTCCCCA GGTGGAACTA CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG 
GCCCAGCGTC AGCCTGTTGG TACATGCATT GCTCGTGTTC GGGCCCTGAG ACGGGCACTG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514903.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514966.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X11, mRNA.

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CloneID	OMu45324
Clone ID Related Accession (Same CDS sequence)	XM_006514903.3 , XM_006514903.4
Accession Version	XM_006514903.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514903.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTG 
GAACTACAGG TCCAGCAGCT GGCTAAGGAG CTTGAGGCCC AGCGTCAGCC TGTTGGTACA 
TGCATTGCTC GTGTTCGGGC CCTGAGACGG GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514966.1
CDS	598..3699
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514903.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514903.4

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTG 
GAACTACAGG TCCAGCAGCT GGCTAAGGAG CTTGAGGCCC AGCGTCAGCC TGTTGGTACA 
TGCATTGCTC GTGTTCGGGC CCTGAGACGG GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( NM_030207.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084483.2 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 1, mRNA.

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CloneID	OMu13878
Clone ID Related Accession (Same CDS sequence)	XM_006514896.3 , NM_030207.3 , NM_030207.2
Accession Version	NM_030207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK639032.1, BY303229.1 and AK133558.1. On May 12, 2018 this sequence version replaced NM_030207.2. ##Evidence-Data-START## Transcript exon combination :: AK133558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTGG AACTACAGGT CCAGCAGCTG GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT 
GTTGGTACAT GCATTGCTCG TGTTCGGGCC CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084483.2
CDS	338..3988
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	NM_030207.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030207.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTGG AACTACAGGT CCAGCAGCTG GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT 
GTTGGTACAT GCATTGCTCG TGTTCGGGCC CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( NM_030207.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084483.2 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), mRNA.

Price & Availability↑

CloneID	OMu13878
Clone ID Related Accession (Same CDS sequence)	XM_006514896.3 , NM_030207.3 , NM_030207.2
Accession Version	NM_030207.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY303229.1 and AK133558.1. On or before Feb 2, 2007 this sequence version replaced XM_906423.2, XM_986858.1, NM_030207.1. ##Evidence-Data-START## Transcript exon combination :: AK133558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTGG AACTACAGGT CCAGCAGCTG GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT 
GTTGGTACAT GCATTGCTCG TGTTCGGGCC CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084483.2
CDS	198..3848
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	NM_030207.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030207.2

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTGG AACTACAGGT CCAGCAGCTG GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT 
GTTGGTACAT GCATTGCTCG TGTTCGGGCC CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514896.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514959.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X4, mRNA.

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CloneID	OMu13878
Clone ID Related Accession (Same CDS sequence)	XM_006514896.3 , NM_030207.3 , NM_030207.2
Accession Version	XM_006514896.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTGG AACTACAGGT CCAGCAGCTG GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT 
GTTGGTACAT GCATTGCTCG TGTTCGGGCC CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514959.1
CDS	594..4244
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514896.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514896.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTGG AACTACAGGT CCAGCAGCTG GCTAAGGAGC TTGAGGCCCA GCGTCAGCCT 
GTTGGTACAT GCATTGCTCG TGTTCGGGCC CTGAGACGGG CACTGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514894.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514957.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45318
Clone ID Related Accession (Same CDS sequence)	XM_006514894.3 , XM_017314842.1 , XM_017314842.2 , XM_017314841.1
Accession Version	XM_006514894.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514957.1
CDS	594..4328
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514894.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514894.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314842.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170331.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu45318
Clone ID Related Accession (Same CDS sequence)	XM_006514894.3 , XM_017314842.1 , XM_017314842.2 , XM_017314841.1
Accession Version	XM_017314842.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170331.1
CDS	969..4703
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314842.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314842.1

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_017314841.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170330.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X2, mRNA.

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CloneID	OMu45318
Clone ID Related Accession (Same CDS sequence)	XM_006514894.3 , XM_017314842.1 , XM_017314842.2 , XM_017314841.1
Accession Version	XM_017314841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170330.1
CDS	1265..4999
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_017314841.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314841.1

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GCAGCCCACA GCCTCCACTG TGCAGTCCGC 
CACTGTGCTG AGCTCTGGAA AAAGAAGGTG CTTGGTCCAG GCAAGACTTC TCAACCACCA 
GCACCCACCA CATTCAGCAA GAGAGTGACA TTTAAGGATT CTTTCCTCTC TGGACATGCT 
GCGGAGGCTG GAGATGCTAC CCAGGAGACA AAGAAGCTTC GAGCTCCTCC ATCTCAGGGA 
GTCCTGGGTA GTTTGGCAGG AGCTGCTGGG GAGCCCTGCC ACCTAGACCT CAATGCAGCC 
CGCTCATCAC GGAAACAGCC CCGACGTCCA TCCTTCCTGC TTGAGCGTTT GGGGAGTCAG 
AGGTCCCCAG AGTGGTACAG CCTTGGGGAG CAGCAGTTAG AGAAGCCCCC AGAGGAGGAA 
AGCACGGCTC TGCTGGGAGG CTCTTCCCTG ACAAGACCCT TCCTACCTGG GGTCCTGCCG 
AATGTTCCTG GTCCGAAGCT GCCTCCTACG GCCAGTCCAG GCCTGGAGCT GCTGCCTCCC 
TCCTCCATCA TGCCCCATGC GGCAGGAGGC ACTGCTAGGG TGTCAGCGAA GCCCAGCATC 
CCTGGGCCTC AGCCTTGGGG GTGCCCGTCC TTACCCAGGG ACCTTGATCC CCAACTCCTC 
CCTGGAGATT CCATAAGCAC CCGGACTGAG CCTGTTTATG GCTCAGAGGC TACAGGCCAC 
ACAGAGCTTG AGGCTGAGTT GGAGGGGATC CAGCAGCAGT TACAACACTA CCAAACCACC 
AAGCAGAACC TTTGGTCATG CCAGCGACAA GCCAACAGCC TGCGTAGGTG GCTGGAGCTA 
AGCCAGGAGG AGCCGAAATC CGAGGACCTT CACCTAGAAG AGCAGGTGAA AACAGAACTG 
GAGGAGGTAA GACCAGGTCA GCCCAGGGCC AGCCCCTGGC TGTCCTTCCT TTCAGCTTGC 
CTTGTCCCTC CCTCTCGCCC ATGTCCCCAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514897.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514960.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X5, mRNA.

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CloneID	OMu45319
Clone ID Related Accession (Same CDS sequence)	XM_006514897.3
Accession Version	XM_006514897.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGG TTTACCAGAA TAGGGTGAGG AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG 
AGACAGCACA ACAGGCAGCT GCTGTGGTGG GCGTTACATC TCTGGAGAGA AAACACCATG 
GCCCGTTTGG ATGGAGCCAA AAAAACCTCT CAAGCAAGAG TTCACTACAG CAGGACTCTG 
TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG GTTACTTCAG TGCAGATATA TTACCGACAA 
AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG AAAGCACTGG ACAGGGGTCG TCTCCAGAAC 
TGGTTTCAGC ACTGGCGATT CTGCAGCCAG AGGGCAGCCC AGCAGAGATT CCAGCTGGGA 
CAGGCGGCTC AGCATCACCA CTGGCAGCTG CTGATGGAAG CAATGGCCAG GTGGAAGGCA 
CACCATCTGG GGTGTATCAG AAAGAAGTTT CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA 
CAGAGACTCA GTCGGGCCTG CTTCTGCCAG TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA 
GAACAGTGGG GCACAGCCCG GGCCCTGTGG CTCTGGGCCT TCTCACTACA AGCAAAGGTG 
TGGACTGCAT GGCTGGGCTT CGTACTTGAG AGGAGGAGGA AGAAGGCACG GCTGGAACGG 
GCTATGCAAG CCTACCAGCA GCAGCTCCTA CAGGAGGGTG CCACACGGCT TCTGCGCTTC 
ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC 
CACAGCCTCC ACTGTGCAGT CCGCCACTGT GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT 
CCAGGCAAGA CTTCTCAACC ACCAGCACCC ACCACATTCA GCAAGAGAGT GACATTTAAG 
GATTCTTTCC TCTCTGGACA TGCTGCGGAG GCTGGAGATG CTACCCAGGA GACAAAGAAG 
CTTCGAGCTC CTCCATCTCA GGGAGTCCTG GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC 
TGCCACCTAG ACCTCAATGC AGCCCGCTCA TCACGGAAAC AGCCCCGACG TCCATCCTTC 
CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG 
TTAGAGAAGC CCCCAGAGGA GGAAAGCACG GCTCTGCTGG GAGGCTCTTC CCTGACAAGA 
CCCTTCCTAC CTGGGGTCCT GCCGAATGTT CCTGGTCCGA AGCTGCCTCC TACGGCCAGT 
CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC ATCATGCCCC ATGCGGCAGG AGGCACTGCT 
AGGGTGTCAG CGAAGCCCAG CATCCCTGGG CCTCAGCCTT GGGGGTGCCC GTCCTTACCC 
AGGGACCTTG ATCCCCAACT CCTCCCTGGA GATTCCATAA GCACCCGGAC TGAGCCTGTT 
TATGGCTCAG AGGCTACAGG CCACACAGAG CTTGAGGCTG AGTTGGAGGG GATCCAGCAG 
CAGTTACAAC ACTACCAAAC CACCAAGCAG AACCTTTGGT CATGCCAGCG ACAAGCCAAC 
AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG GAGGAGCCGA AATCCGAGGA CCTTCACCTA 
GAAGAGCAGG TGAAAACAGA ACTGGAGGAG GTAAGACCAG GTCAGCCCAG GGCCAGCCCC 
TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC CCTCCCTCTC GCCCATGTCC CCAGGTGGAA 
CTACAGGTCC AGCAGCTGGC TAAGGAGCTT GAGGCCCAGC GTCAGCCTGT TGGTACATGC 
ATTGCTCGTG TTCGGGCCCT GAGACGGGCA CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514960.1
CDS	594..4232
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514897.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514897.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGG TTTACCAGAA TAGGGTGAGG AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG 
AGACAGCACA ACAGGCAGCT GCTGTGGTGG GCGTTACATC TCTGGAGAGA AAACACCATG 
GCCCGTTTGG ATGGAGCCAA AAAAACCTCT CAAGCAAGAG TTCACTACAG CAGGACTCTG 
TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG GTTACTTCAG TGCAGATATA TTACCGACAA 
AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG AAAGCACTGG ACAGGGGTCG TCTCCAGAAC 
TGGTTTCAGC ACTGGCGATT CTGCAGCCAG AGGGCAGCCC AGCAGAGATT CCAGCTGGGA 
CAGGCGGCTC AGCATCACCA CTGGCAGCTG CTGATGGAAG CAATGGCCAG GTGGAAGGCA 
CACCATCTGG GGTGTATCAG AAAGAAGTTT CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA 
CAGAGACTCA GTCGGGCCTG CTTCTGCCAG TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA 
GAACAGTGGG GCACAGCCCG GGCCCTGTGG CTCTGGGCCT TCTCACTACA AGCAAAGGTG 
TGGACTGCAT GGCTGGGCTT CGTACTTGAG AGGAGGAGGA AGAAGGCACG GCTGGAACGG 
GCTATGCAAG CCTACCAGCA GCAGCTCCTA CAGGAGGGTG CCACACGGCT TCTGCGCTTC 
ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC 
CACAGCCTCC ACTGTGCAGT CCGCCACTGT GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT 
CCAGGCAAGA CTTCTCAACC ACCAGCACCC ACCACATTCA GCAAGAGAGT GACATTTAAG 
GATTCTTTCC TCTCTGGACA TGCTGCGGAG GCTGGAGATG CTACCCAGGA GACAAAGAAG 
CTTCGAGCTC CTCCATCTCA GGGAGTCCTG GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC 
TGCCACCTAG ACCTCAATGC AGCCCGCTCA TCACGGAAAC AGCCCCGACG TCCATCCTTC 
CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG 
TTAGAGAAGC CCCCAGAGGA GGAAAGCACG GCTCTGCTGG GAGGCTCTTC CCTGACAAGA 
CCCTTCCTAC CTGGGGTCCT GCCGAATGTT CCTGGTCCGA AGCTGCCTCC TACGGCCAGT 
CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC ATCATGCCCC ATGCGGCAGG AGGCACTGCT 
AGGGTGTCAG CGAAGCCCAG CATCCCTGGG CCTCAGCCTT GGGGGTGCCC GTCCTTACCC 
AGGGACCTTG ATCCCCAACT CCTCCCTGGA GATTCCATAA GCACCCGGAC TGAGCCTGTT 
TATGGCTCAG AGGCTACAGG CCACACAGAG CTTGAGGCTG AGTTGGAGGG GATCCAGCAG 
CAGTTACAAC ACTACCAAAC CACCAAGCAG AACCTTTGGT CATGCCAGCG ACAAGCCAAC 
AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG GAGGAGCCGA AATCCGAGGA CCTTCACCTA 
GAAGAGCAGG TGAAAACAGA ACTGGAGGAG GTAAGACCAG GTCAGCCCAG GGCCAGCCCC 
TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC CCTCCCTCTC GCCCATGTCC CCAGGTGGAA 
CTACAGGTCC AGCAGCTGGC TAAGGAGCTT GAGGCCCAGC GTCAGCCTGT TGGTACATGC 
ATTGCTCGTG TTCGGGCCCT GAGACGGGCA CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514898.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514961.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu45320
Clone ID Related Accession (Same CDS sequence)	XM_006514898.3
Accession Version	XM_006514898.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514898.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGG TTTACCAGAA TAGGGTGAGG AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG 
AGACAGCACA ACAGGCAGCT GCTGTGGTGG GCGTTACATC TCTGGAGAGA AAACACCATG 
GCCCGTTTGG ATGGAGCCAA AAAAACCTCT CAAGCAAGAG TTCACTACAG CAGGACTCTG 
TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG GTTACTTCAG TGCAGATATA TTACCGACAA 
AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG AAAGCACTGG ACAGGGGTCG TCTCCAGAAC 
TGGTTTCAGC ACTGGCGATT CTGCAGCCAG AGGGCAGCCC AGCAGAGATT CCAGCTGGGA 
CAGGCGGCTC AGCATCACCA CTGGCAGCTG CTGATGGAAG CAATGGCCAG GTGGAAGGCA 
CACCATCTGG GGTGTATCAG AAAGAAGTTT CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA 
CAGAGACTCA GTCGGGCCTG CTTCTGCCAG TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA 
GAACAGTGGG GCACAGCCCG GGCCCTGTGG CTCTGGGCCT TCTCACTACA AGCAAAGGTG 
TGGACTGCAT GGCTGGGCTT CGTACTTGAG AGGAGGAGGA AGAAGGCACG GCTGGAACGG 
GCTATGCAAG CCTACCAGCA GCAGCTCCTA CAGGAGGGTG CCACACGGCT TCTGCGCTTC 
ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC 
CACAGCCTCC ACTGTGCAGT CCGCCACTGT GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT 
CCAGGCAAGA CTTCTCAACC ACCAGCACCC ACCACATTCA GCAAGAGAGT GACATTTAAG 
GATTCTTTCC TCTCTGGACA TGCTGCGGAG GCTGGAGATG CTACCCAGGA GACAAAGAAG 
CTTCGAGCTC CTCCATCTCA GGGAGTCCTG GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC 
TGCCACCTAG ACCTCAATGC AGCCCGCTCA TCACGGAAAC AGCCCCGACG TCCATCCTTC 
CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG 
TTAGAGAAGC CCCCAGAGGA GGAAAGCACG GCTCTGCTGG GAGGCTCTTC CCTGACAAGA 
CCCTTCCTAC CTGGGGTCCT GCCGAATGTT CCTGGTCCGA AGCTGCCTCC TACGGCCAGT 
CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC ATCATGCCCC ATGCGGCAGG AGGCACTGCT 
AGGGTGTCAG CGAAGCCCAG CATCCCTGGG CCTCAGCCTT GGGGGTGCCC GTCCTTACCC 
AGGGACCTTG ATCCCCAACT CCTCCCTGGA GATTCCATAA GCACCCGGAC TGAGCCTGTT 
TATGGCTCAG AGGCTACAGG CCACACAGAG CTTGAGGCTG AGTTGGAGGG GATCCAGCAG 
CAGTTACAAC ACTACCAAAC CACCAAGCAG AACCTTTGGT CATGCCAGCG ACAAGCCAAC 
AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG GAGGAGCCGA AATCCGAGGA CCTTCACCTA 
GAAGAGCAGG TGAAAACAGA ACTGGAGGAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514961.1
CDS	594..4148
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514898.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514898.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGG TTTACCAGAA TAGGGTGAGG AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG 
AGACAGCACA ACAGGCAGCT GCTGTGGTGG GCGTTACATC TCTGGAGAGA AAACACCATG 
GCCCGTTTGG ATGGAGCCAA AAAAACCTCT CAAGCAAGAG TTCACTACAG CAGGACTCTG 
TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG GTTACTTCAG TGCAGATATA TTACCGACAA 
AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG AAAGCACTGG ACAGGGGTCG TCTCCAGAAC 
TGGTTTCAGC ACTGGCGATT CTGCAGCCAG AGGGCAGCCC AGCAGAGATT CCAGCTGGGA 
CAGGCGGCTC AGCATCACCA CTGGCAGCTG CTGATGGAAG CAATGGCCAG GTGGAAGGCA 
CACCATCTGG GGTGTATCAG AAAGAAGTTT CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA 
CAGAGACTCA GTCGGGCCTG CTTCTGCCAG TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA 
GAACAGTGGG GCACAGCCCG GGCCCTGTGG CTCTGGGCCT TCTCACTACA AGCAAAGGTG 
TGGACTGCAT GGCTGGGCTT CGTACTTGAG AGGAGGAGGA AGAAGGCACG GCTGGAACGG 
GCTATGCAAG CCTACCAGCA GCAGCTCCTA CAGGAGGGTG CCACACGGCT TCTGCGCTTC 
ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC 
CACAGCCTCC ACTGTGCAGT CCGCCACTGT GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT 
CCAGGCAAGA CTTCTCAACC ACCAGCACCC ACCACATTCA GCAAGAGAGT GACATTTAAG 
GATTCTTTCC TCTCTGGACA TGCTGCGGAG GCTGGAGATG CTACCCAGGA GACAAAGAAG 
CTTCGAGCTC CTCCATCTCA GGGAGTCCTG GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC 
TGCCACCTAG ACCTCAATGC AGCCCGCTCA TCACGGAAAC AGCCCCGACG TCCATCCTTC 
CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG 
TTAGAGAAGC CCCCAGAGGA GGAAAGCACG GCTCTGCTGG GAGGCTCTTC CCTGACAAGA 
CCCTTCCTAC CTGGGGTCCT GCCGAATGTT CCTGGTCCGA AGCTGCCTCC TACGGCCAGT 
CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC ATCATGCCCC ATGCGGCAGG AGGCACTGCT 
AGGGTGTCAG CGAAGCCCAG CATCCCTGGG CCTCAGCCTT GGGGGTGCCC GTCCTTACCC 
AGGGACCTTG ATCCCCAACT CCTCCCTGGA GATTCCATAA GCACCCGGAC TGAGCCTGTT 
TATGGCTCAG AGGCTACAGG CCACACAGAG CTTGAGGCTG AGTTGGAGGG GATCCAGCAG 
CAGTTACAAC ACTACCAAAC CACCAAGCAG AACCTTTGGT CATGCCAGCG ACAAGCCAAC 
AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG GAGGAGCCGA AATCCGAGGA CCTTCACCTA 
GAAGAGCAGG TGAAAACAGA ACTGGAGGAG GTGGAACTAC AGGTCCAGCA GCTGGCTAAG 
GAGCTTGAGG CCCAGCGTCA GCCTGTTGGT ACATGCATTG CTCGTGTTCG GGCCCTGAGA 
CGGGCACTGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514900.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514963.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu45321
Clone ID Related Accession (Same CDS sequence)	XM_006514900.3 , XM_006514899.3
Accession Version	XM_006514900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGA GGTTCCGAAT AGCTGAGCAT CATGATACGA AGCAGAAGAT GTGCCAAGCC 
TGGAAGTCCT GGTTGATATA CATGGTTTCT CGTAGGACCA AACTTCACAT GAAGACTACT 
GCTCTAGAGT TTAGGAGGCA GAGTGTCTTA TGTTTCTGGT GGAGCAAGTG GAGGTGGCGA 
CTAGGACAAG CCCATGCAGA GCACGCTCTC CATGCAGTGG CTGTGAAGCA CAGGGCCCTG 
AGCCTCCAGC TGCAGGGTTG GCTGCGATGG CAAGAACAGC TTCTAATTAG CCAGCGAGAT 
AGGAGGAAGG AGGCCACTGC TGTGCAACAC TATCAGCACT GGCAGAAGCA GAGATCTCTG 
AAGGCTTGGC TTAAATACCT CCAAATCTGC AGGGTGAAGA GATGGCAGAA TGAGATGGCT 
GTACAATTCC ACCGTGCTAC TGTGCTCCAG ATACATTTCT GTGACTGGCA GTGGGCCTGG 
GAGTGGAGGC AGAGCTTGTC TGCCCACCAG GCCCTGGTGG TGAAGCTGGC CGGGAGGATG 
GTCCTGCGGA GGGCCTTTAC ACACTGGAAG CACTACATGT TGCTACAGGC AGAAGAGGCT 
GCCCAGCGCG AGGCAGCAGC TGAACACCGC CAGCACTACC TGCTGTATTC TTGCTTTAGA 
GCCTTCAAAG ACAACGTGAC CCAGGCCCGA CTACAGCAAA CAAGAAAGAA GCTTGCTCAG 
CAGCTGCGTG ACACTACATT GCTTCACAGG TTCTGGAACC TCTGGCAGTC TCGAATTGAG 
CAGAGGGAGG AGAGGGTACA GACCCCCTCA TTGCATGCAG CCCTGAGCCA CTACAGGGTG 
ACAGTCCTAC ACAAGTGTGT CAGGGTGTGG CTACGGTATG TGCACAAGAG ACAATGGCAG 
CAGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GTGCCTGGCC 
CTGAGGCTGG AAGAGCAGCA GAAACTGAAG TGTGCGGCCC TGCACAGCCA GTGCATCCTG 
CTTCGCAGGG CTCTGCAGAA GTGGCTGGTT TACCAGAATA GGGTGAGGAG TGTTCTGCGG 
GAGGTGGCGG CCAGGGAGAG ACAGCACAAC AGGCAGCTGC TGTGGTGGGC GTTACATCTC 
TGGAGAGAAA ACACCATGGC CCGTTTGGAT GGAGCCAAAA AAACCTCTCA AGCAAGAGTT 
CACTACAGCA GGACTCTGTG CTCCAAGGTC CTCGTCCAGT GGCGAGAGGT TACTTCAGTG 
CAGATATATT ACCGACAAAA GGAGGCAGCT GCCCTCAGGG AGGCCCGGAA AGCACTGGAC 
AGGGGTCGTC TCCAGAACTG GTTTCAGCAC TGGCGATTCT GCAGCCAGAG GGCAGCCCAG 
CAGAGATTCC AGCTGGGACA GGCGGCTCAG CATCACCACT GGCAGCTGCT GATGGAAGCA 
ATGGCCAGGT GGAAGGCACA CCATCTGGGG TGTATCAGAA AGAAGTTTCT GCAGAGGCAA 
GCTGCCCAAC TCCTGGCACA GAGACTCAGT CGGGCCTGCT TCTGCCAGTG GAGGAAGCAG 
CTGGCAGTCC GGAAGCAAGA ACAGTGGGGC ACAGCCCGGG CCCTGTGGCT CTGGGCCTTC 
TCACTACAAG CAAAGGTGTG GACTGCATGG CTGGGCTTCG TACTTGAGAG GAGGAGGAAG 
AAGGCACGGC TGGAACGGGC TATGCAAGCC TACCAGCAGC AGCTCCTACA GGAGGGTGCC 
ACACGGCTTC TGCGCTTCAC AGCTGGTACG AAGGCCTTCC GGCAGCAGCT CCAGGCCCAG 
CAGCAGGTCC AGGCAGCCCA CAGCCTCCAC TGTGCAGTCC GCCACTGTGC TGAGCTCTGG 
AAAAAGAAGG TGCTTGGTCC AGGCAAGACT TCTCAACCAC CAGCACCCAC CACATTCAGC 
AAGAGAGTGA CATTTAAGGA TTCTTTCCTC TCTGGACATG CTGCGGAGGC TGGAGATGCT 
ACCCAGGAGA CAAAGAAGCT TCGAGCTCCT CCATCTCAGG GAGTCCTGGG TAGTTTGGCA 
GGAGCTGCTG GGGAGCCCTG CCACCTAGAC CTCAATGCAG CCCGCTCATC ACGGAAACAG 
CCCCGACGTC CATCCTTCCT GCTTGAGCGT TTGGGGAGTC AGAGGTCCCC AGAGTGGTAC 
AGCCTTGGGG AGCAGCAGTT AGAGAAGCCC CCAGAGGAGG AAAGCACGGC TCTGCTGGGA 
GGCTCTTCCC TGACAAGACC CTTCCTACCT GGGGTCCTGC CGAATGTTCC TGGTCCGAAG 
CTGCCTCCTA CGGCCAGTCC AGGCCTGGAG CTGCTGCCTC CCTCCTCCAT CATGCCCCAT 
GCGGCAGGAG GCACTGCTAG GGTGTCAGCG AAGCCCAGCA TCCCTGGGCC TCAGCCTTGG 
GGGTGCCCGT CCTTACCCAG GGACCTTGAT CCCCAACTCC TCCCTGGAGA TTCCATAAGC 
ACCCGGACTG AGCCTGTTTA TGGCTCAGAG GCTACAGGCC ACACAGAGCT TGAGGCTGAG 
TTGGAGGGGA TCCAGCAGCA GTTACAACAC TACCAAACCA CCAAGCAGAA CCTTTGGTCA 
TGCCAGCGAC AAGCCAACAG CCTGCGTAGG TGGCTGGAGC TAAGCCAGGA GGAGCCGAAA 
TCCGAGGACC TTCACCTAGA AGAGCAGGTG AAAACAGAAC TGGAGGAGGT AAGACCAGGT 
CAGCCCAGGG CCAGCCCCTG GCTGTCCTTC CTTTCAGCTT GCCTTGTCCC TCCCTCTCGC 
CCATGTCCCC AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514963.1
CDS	916..4212
Translation	MRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514900.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514900.3

ORF Insert Sequence:

ATGAGGAAGA GGTTCCGAAT AGCTGAGCAT CATGATACGA AGCAGAAGAT GTGCCAAGCC 
TGGAAGTCCT GGTTGATATA CATGGTTTCT CGTAGGACCA AACTTCACAT GAAGACTACT 
GCTCTAGAGT TTAGGAGGCA GAGTGTCTTA TGTTTCTGGT GGAGCAAGTG GAGGTGGCGA 
CTAGGACAAG CCCATGCAGA GCACGCTCTC CATGCAGTGG CTGTGAAGCA CAGGGCCCTG 
AGCCTCCAGC TGCAGGGTTG GCTGCGATGG CAAGAACAGC TTCTAATTAG CCAGCGAGAT 
AGGAGGAAGG AGGCCACTGC TGTGCAACAC TATCAGCACT GGCAGAAGCA GAGATCTCTG 
AAGGCTTGGC TTAAATACCT CCAAATCTGC AGGGTGAAGA GATGGCAGAA TGAGATGGCT 
GTACAATTCC ACCGTGCTAC TGTGCTCCAG ATACATTTCT GTGACTGGCA GTGGGCCTGG 
GAGTGGAGGC AGAGCTTGTC TGCCCACCAG GCCCTGGTGG TGAAGCTGGC CGGGAGGATG 
GTCCTGCGGA GGGCCTTTAC ACACTGGAAG CACTACATGT TGCTACAGGC AGAAGAGGCT 
GCCCAGCGCG AGGCAGCAGC TGAACACCGC CAGCACTACC TGCTGTATTC TTGCTTTAGA 
GCCTTCAAAG ACAACGTGAC CCAGGCCCGA CTACAGCAAA CAAGAAAGAA GCTTGCTCAG 
CAGCTGCGTG ACACTACATT GCTTCACAGG TTCTGGAACC TCTGGCAGTC TCGAATTGAG 
CAGAGGGAGG AGAGGGTACA GACCCCCTCA TTGCATGCAG CCCTGAGCCA CTACAGGGTG 
ACAGTCCTAC ACAAGTGTGT CAGGGTGTGG CTACGGTATG TGCACAAGAG ACAATGGCAG 
CAGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GTGCCTGGCC 
CTGAGGCTGG AAGAGCAGCA GAAACTGAAG TGTGCGGCCC TGCACAGCCA GTGCATCCTG 
CTTCGCAGGG CTCTGCAGAA GTGGCTGGTT TACCAGAATA GGGTGAGGAG TGTTCTGCGG 
GAGGTGGCGG CCAGGGAGAG ACAGCACAAC AGGCAGCTGC TGTGGTGGGC GTTACATCTC 
TGGAGAGAAA ACACCATGGC CCGTTTGGAT GGAGCCAAAA AAACCTCTCA AGCAAGAGTT 
CACTACAGCA GGACTCTGTG CTCCAAGGTC CTCGTCCAGT GGCGAGAGGT TACTTCAGTG 
CAGATATATT ACCGACAAAA GGAGGCAGCT GCCCTCAGGG AGGCCCGGAA AGCACTGGAC 
AGGGGTCGTC TCCAGAACTG GTTTCAGCAC TGGCGATTCT GCAGCCAGAG GGCAGCCCAG 
CAGAGATTCC AGCTGGGACA GGCGGCTCAG CATCACCACT GGCAGCTGCT GATGGAAGCA 
ATGGCCAGGT GGAAGGCACA CCATCTGGGG TGTATCAGAA AGAAGTTTCT GCAGAGGCAA 
GCTGCCCAAC TCCTGGCACA GAGACTCAGT CGGGCCTGCT TCTGCCAGTG GAGGAAGCAG 
CTGGCAGTCC GGAAGCAAGA ACAGTGGGGC ACAGCCCGGG CCCTGTGGCT CTGGGCCTTC 
TCACTACAAG CAAAGGTGTG GACTGCATGG CTGGGCTTCG TACTTGAGAG GAGGAGGAAG 
AAGGCACGGC TGGAACGGGC TATGCAAGCC TACCAGCAGC AGCTCCTACA GGAGGGTGCC 
ACACGGCTTC TGCGCTTCAC AGCTGGTACG AAGGCCTTCC GGCAGCAGCT CCAGGCCCAG 
CAGCAGGTCC AGGCAGCCCA CAGCCTCCAC TGTGCAGTCC GCCACTGTGC TGAGCTCTGG 
AAAAAGAAGG TGCTTGGTCC AGGCAAGACT TCTCAACCAC CAGCACCCAC CACATTCAGC 
AAGAGAGTGA CATTTAAGGA TTCTTTCCTC TCTGGACATG CTGCGGAGGC TGGAGATGCT 
ACCCAGGAGA CAAAGAAGCT TCGAGCTCCT CCATCTCAGG GAGTCCTGGG TAGTTTGGCA 
GGAGCTGCTG GGGAGCCCTG CCACCTAGAC CTCAATGCAG CCCGCTCATC ACGGAAACAG 
CCCCGACGTC CATCCTTCCT GCTTGAGCGT TTGGGGAGTC AGAGGTCCCC AGAGTGGTAC 
AGCCTTGGGG AGCAGCAGTT AGAGAAGCCC CCAGAGGAGG AAAGCACGGC TCTGCTGGGA 
GGCTCTTCCC TGACAAGACC CTTCCTACCT GGGGTCCTGC CGAATGTTCC TGGTCCGAAG 
CTGCCTCCTA CGGCCAGTCC AGGCCTGGAG CTGCTGCCTC CCTCCTCCAT CATGCCCCAT 
GCGGCAGGAG GCACTGCTAG GGTGTCAGCG AAGCCCAGCA TCCCTGGGCC TCAGCCTTGG 
GGGTGCCCGT CCTTACCCAG GGACCTTGAT CCCCAACTCC TCCCTGGAGA TTCCATAAGC 
ACCCGGACTG AGCCTGTTTA TGGCTCAGAG GCTACAGGCC ACACAGAGCT TGAGGCTGAG 
TTGGAGGGGA TCCAGCAGCA GTTACAACAC TACCAAACCA CCAAGCAGAA CCTTTGGTCA 
TGCCAGCGAC AAGCCAACAG CCTGCGTAGG TGGCTGGAGC TAAGCCAGGA GGAGCCGAAA 
TCCGAGGACC TTCACCTAGA AGAGCAGGTG AAAACAGAAC TGGAGGAGGT AAGACCAGGT 
CAGCCCAGGG CCAGCCCCTG GCTGTCCTTC CTTTCAGCTT GCCTTGTCCC TCCCTCTCGC 
CCATGTCCCC AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514899.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514962.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu45321
Clone ID Related Accession (Same CDS sequence)	XM_006514900.3 , XM_006514899.3
Accession Version	XM_006514899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGA GGTTCCGAAT AGCTGAGCAT CATGATACGA AGCAGAAGAT GTGCCAAGCC 
TGGAAGTCCT GGTTGATATA CATGGTTTCT CGTAGGACCA AACTTCACAT GAAGACTACT 
GCTCTAGAGT TTAGGAGGCA GAGTGTCTTA TGTTTCTGGT GGAGCAAGTG GAGGTGGCGA 
CTAGGACAAG CCCATGCAGA GCACGCTCTC CATGCAGTGG CTGTGAAGCA CAGGGCCCTG 
AGCCTCCAGC TGCAGGGTTG GCTGCGATGG CAAGAACAGC TTCTAATTAG CCAGCGAGAT 
AGGAGGAAGG AGGCCACTGC TGTGCAACAC TATCAGCACT GGCAGAAGCA GAGATCTCTG 
AAGGCTTGGC TTAAATACCT CCAAATCTGC AGGGTGAAGA GATGGCAGAA TGAGATGGCT 
GTACAATTCC ACCGTGCTAC TGTGCTCCAG ATACATTTCT GTGACTGGCA GTGGGCCTGG 
GAGTGGAGGC AGAGCTTGTC TGCCCACCAG GCCCTGGTGG TGAAGCTGGC CGGGAGGATG 
GTCCTGCGGA GGGCCTTTAC ACACTGGAAG CACTACATGT TGCTACAGGC AGAAGAGGCT 
GCCCAGCGCG AGGCAGCAGC TGAACACCGC CAGCACTACC TGCTGTATTC TTGCTTTAGA 
GCCTTCAAAG ACAACGTGAC CCAGGCCCGA CTACAGCAAA CAAGAAAGAA GCTTGCTCAG 
CAGCTGCGTG ACACTACATT GCTTCACAGG TTCTGGAACC TCTGGCAGTC TCGAATTGAG 
CAGAGGGAGG AGAGGGTACA GACCCCCTCA TTGCATGCAG CCCTGAGCCA CTACAGGGTG 
ACAGTCCTAC ACAAGTGTGT CAGGGTGTGG CTACGGTATG TGCACAAGAG ACAATGGCAG 
CAGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GTGCCTGGCC 
CTGAGGCTGG AAGAGCAGCA GAAACTGAAG TGTGCGGCCC TGCACAGCCA GTGCATCCTG 
CTTCGCAGGG CTCTGCAGAA GTGGCTGGTT TACCAGAATA GGGTGAGGAG TGTTCTGCGG 
GAGGTGGCGG CCAGGGAGAG ACAGCACAAC AGGCAGCTGC TGTGGTGGGC GTTACATCTC 
TGGAGAGAAA ACACCATGGC CCGTTTGGAT GGAGCCAAAA AAACCTCTCA AGCAAGAGTT 
CACTACAGCA GGACTCTGTG CTCCAAGGTC CTCGTCCAGT GGCGAGAGGT TACTTCAGTG 
CAGATATATT ACCGACAAAA GGAGGCAGCT GCCCTCAGGG AGGCCCGGAA AGCACTGGAC 
AGGGGTCGTC TCCAGAACTG GTTTCAGCAC TGGCGATTCT GCAGCCAGAG GGCAGCCCAG 
CAGAGATTCC AGCTGGGACA GGCGGCTCAG CATCACCACT GGCAGCTGCT GATGGAAGCA 
ATGGCCAGGT GGAAGGCACA CCATCTGGGG TGTATCAGAA AGAAGTTTCT GCAGAGGCAA 
GCTGCCCAAC TCCTGGCACA GAGACTCAGT CGGGCCTGCT TCTGCCAGTG GAGGAAGCAG 
CTGGCAGTCC GGAAGCAAGA ACAGTGGGGC ACAGCCCGGG CCCTGTGGCT CTGGGCCTTC 
TCACTACAAG CAAAGGTGTG GACTGCATGG CTGGGCTTCG TACTTGAGAG GAGGAGGAAG 
AAGGCACGGC TGGAACGGGC TATGCAAGCC TACCAGCAGC AGCTCCTACA GGAGGGTGCC 
ACACGGCTTC TGCGCTTCAC AGCTGGTACG AAGGCCTTCC GGCAGCAGCT CCAGGCCCAG 
CAGCAGGTCC AGGCAGCCCA CAGCCTCCAC TGTGCAGTCC GCCACTGTGC TGAGCTCTGG 
AAAAAGAAGG TGCTTGGTCC AGGCAAGACT TCTCAACCAC CAGCACCCAC CACATTCAGC 
AAGAGAGTGA CATTTAAGGA TTCTTTCCTC TCTGGACATG CTGCGGAGGC TGGAGATGCT 
ACCCAGGAGA CAAAGAAGCT TCGAGCTCCT CCATCTCAGG GAGTCCTGGG TAGTTTGGCA 
GGAGCTGCTG GGGAGCCCTG CCACCTAGAC CTCAATGCAG CCCGCTCATC ACGGAAACAG 
CCCCGACGTC CATCCTTCCT GCTTGAGCGT TTGGGGAGTC AGAGGTCCCC AGAGTGGTAC 
AGCCTTGGGG AGCAGCAGTT AGAGAAGCCC CCAGAGGAGG AAAGCACGGC TCTGCTGGGA 
GGCTCTTCCC TGACAAGACC CTTCCTACCT GGGGTCCTGC CGAATGTTCC TGGTCCGAAG 
CTGCCTCCTA CGGCCAGTCC AGGCCTGGAG CTGCTGCCTC CCTCCTCCAT CATGCCCCAT 
GCGGCAGGAG GCACTGCTAG GGTGTCAGCG AAGCCCAGCA TCCCTGGGCC TCAGCCTTGG 
GGGTGCCCGT CCTTACCCAG GGACCTTGAT CCCCAACTCC TCCCTGGAGA TTCCATAAGC 
ACCCGGACTG AGCCTGTTTA TGGCTCAGAG GCTACAGGCC ACACAGAGCT TGAGGCTGAG 
TTGGAGGGGA TCCAGCAGCA GTTACAACAC TACCAAACCA CCAAGCAGAA CCTTTGGTCA 
TGCCAGCGAC AAGCCAACAG CCTGCGTAGG TGGCTGGAGC TAAGCCAGGA GGAGCCGAAA 
TCCGAGGACC TTCACCTAGA AGAGCAGGTG AAAACAGAAC TGGAGGAGGT AAGACCAGGT 
CAGCCCAGGG CCAGCCCCTG GCTGTCCTTC CTTTCAGCTT GCCTTGTCCC TCCCTCTCGC 
CCATGTCCCC AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514962.1
CDS	1095..4391
Translation	MRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514899.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514899.3

ORF Insert Sequence:

ATGAGGAAGA GGTTCCGAAT AGCTGAGCAT CATGATACGA AGCAGAAGAT GTGCCAAGCC 
TGGAAGTCCT GGTTGATATA CATGGTTTCT CGTAGGACCA AACTTCACAT GAAGACTACT 
GCTCTAGAGT TTAGGAGGCA GAGTGTCTTA TGTTTCTGGT GGAGCAAGTG GAGGTGGCGA 
CTAGGACAAG CCCATGCAGA GCACGCTCTC CATGCAGTGG CTGTGAAGCA CAGGGCCCTG 
AGCCTCCAGC TGCAGGGTTG GCTGCGATGG CAAGAACAGC TTCTAATTAG CCAGCGAGAT 
AGGAGGAAGG AGGCCACTGC TGTGCAACAC TATCAGCACT GGCAGAAGCA GAGATCTCTG 
AAGGCTTGGC TTAAATACCT CCAAATCTGC AGGGTGAAGA GATGGCAGAA TGAGATGGCT 
GTACAATTCC ACCGTGCTAC TGTGCTCCAG ATACATTTCT GTGACTGGCA GTGGGCCTGG 
GAGTGGAGGC AGAGCTTGTC TGCCCACCAG GCCCTGGTGG TGAAGCTGGC CGGGAGGATG 
GTCCTGCGGA GGGCCTTTAC ACACTGGAAG CACTACATGT TGCTACAGGC AGAAGAGGCT 
GCCCAGCGCG AGGCAGCAGC TGAACACCGC CAGCACTACC TGCTGTATTC TTGCTTTAGA 
GCCTTCAAAG ACAACGTGAC CCAGGCCCGA CTACAGCAAA CAAGAAAGAA GCTTGCTCAG 
CAGCTGCGTG ACACTACATT GCTTCACAGG TTCTGGAACC TCTGGCAGTC TCGAATTGAG 
CAGAGGGAGG AGAGGGTACA GACCCCCTCA TTGCATGCAG CCCTGAGCCA CTACAGGGTG 
ACAGTCCTAC ACAAGTGTGT CAGGGTGTGG CTACGGTATG TGCACAAGAG ACAATGGCAG 
CAGTTATTGC GGGCCAGAGC AGATGGTCAT TTCCAGCAGC GAGCACTGCC TGCTGCCTTC 
TACACGTGGT ACAGAGGCTG GCTATGGCAC CAGCAGAGAC GGATCCTGCA CACAAAAGCA 
GTGCGTTTCC ACAGAGGGAC ATTAGAGAAG CAAGTATTTG CTCTCTGGAG GCAGAAGATG 
TCTCAGCATC GAGAAAACTG CTTGGCAGAG AGAATGGCTA TACTGCAGGC AGAGCAGCAG 
CTTTTGCGTA GGTTCTGGTT CGTGTGGCAC CAGCAGGCAG CAGTGTGTCA GCTGGAGCGA 
CAGCAGCAAG CCATGGCCAT TGCCCACCAC CACAGTGGAC TGCTCAGGAG GGCTTTCTGC 
ATCTGGAAGG AAAGCACCCA AGGATTTAGG ATAGAGAGGA TGGGCAGGGC ACAGGCTGCG 
CACTTCCACT CTGCACAGCT CTTGAGTCGG GCCTGGAGCA TGTGGAGGGA GTGCCTGGCC 
CTGAGGCTGG AAGAGCAGCA GAAACTGAAG TGTGCGGCCC TGCACAGCCA GTGCATCCTG 
CTTCGCAGGG CTCTGCAGAA GTGGCTGGTT TACCAGAATA GGGTGAGGAG TGTTCTGCGG 
GAGGTGGCGG CCAGGGAGAG ACAGCACAAC AGGCAGCTGC TGTGGTGGGC GTTACATCTC 
TGGAGAGAAA ACACCATGGC CCGTTTGGAT GGAGCCAAAA AAACCTCTCA AGCAAGAGTT 
CACTACAGCA GGACTCTGTG CTCCAAGGTC CTCGTCCAGT GGCGAGAGGT TACTTCAGTG 
CAGATATATT ACCGACAAAA GGAGGCAGCT GCCCTCAGGG AGGCCCGGAA AGCACTGGAC 
AGGGGTCGTC TCCAGAACTG GTTTCAGCAC TGGCGATTCT GCAGCCAGAG GGCAGCCCAG 
CAGAGATTCC AGCTGGGACA GGCGGCTCAG CATCACCACT GGCAGCTGCT GATGGAAGCA 
ATGGCCAGGT GGAAGGCACA CCATCTGGGG TGTATCAGAA AGAAGTTTCT GCAGAGGCAA 
GCTGCCCAAC TCCTGGCACA GAGACTCAGT CGGGCCTGCT TCTGCCAGTG GAGGAAGCAG 
CTGGCAGTCC GGAAGCAAGA ACAGTGGGGC ACAGCCCGGG CCCTGTGGCT CTGGGCCTTC 
TCACTACAAG CAAAGGTGTG GACTGCATGG CTGGGCTTCG TACTTGAGAG GAGGAGGAAG 
AAGGCACGGC TGGAACGGGC TATGCAAGCC TACCAGCAGC AGCTCCTACA GGAGGGTGCC 
ACACGGCTTC TGCGCTTCAC AGCTGGTACG AAGGCCTTCC GGCAGCAGCT CCAGGCCCAG 
CAGCAGGTCC AGGCAGCCCA CAGCCTCCAC TGTGCAGTCC GCCACTGTGC TGAGCTCTGG 
AAAAAGAAGG TGCTTGGTCC AGGCAAGACT TCTCAACCAC CAGCACCCAC CACATTCAGC 
AAGAGAGTGA CATTTAAGGA TTCTTTCCTC TCTGGACATG CTGCGGAGGC TGGAGATGCT 
ACCCAGGAGA CAAAGAAGCT TCGAGCTCCT CCATCTCAGG GAGTCCTGGG TAGTTTGGCA 
GGAGCTGCTG GGGAGCCCTG CCACCTAGAC CTCAATGCAG CCCGCTCATC ACGGAAACAG 
CCCCGACGTC CATCCTTCCT GCTTGAGCGT TTGGGGAGTC AGAGGTCCCC AGAGTGGTAC 
AGCCTTGGGG AGCAGCAGTT AGAGAAGCCC CCAGAGGAGG AAAGCACGGC TCTGCTGGGA 
GGCTCTTCCC TGACAAGACC CTTCCTACCT GGGGTCCTGC CGAATGTTCC TGGTCCGAAG 
CTGCCTCCTA CGGCCAGTCC AGGCCTGGAG CTGCTGCCTC CCTCCTCCAT CATGCCCCAT 
GCGGCAGGAG GCACTGCTAG GGTGTCAGCG AAGCCCAGCA TCCCTGGGCC TCAGCCTTGG 
GGGTGCCCGT CCTTACCCAG GGACCTTGAT CCCCAACTCC TCCCTGGAGA TTCCATAAGC 
ACCCGGACTG AGCCTGTTTA TGGCTCAGAG GCTACAGGCC ACACAGAGCT TGAGGCTGAG 
TTGGAGGGGA TCCAGCAGCA GTTACAACAC TACCAAACCA CCAAGCAGAA CCTTTGGTCA 
TGCCAGCGAC AAGCCAACAG CCTGCGTAGG TGGCTGGAGC TAAGCCAGGA GGAGCCGAAA 
TCCGAGGACC TTCACCTAGA AGAGCAGGTG AAAACAGAAC TGGAGGAGGT AAGACCAGGT 
CAGCCCAGGG CCAGCCCCTG GCTGTCCTTC CTTTCAGCTT GCCTTGTCCC TCCCTCTCGC 
CCATGTCCCC AGGTGGAACT ACAGGTCCAG CAGCTGGCTA AGGAGCTTGA GGCCCAGCGT 
CAGCCTGTTG GTACATGCAT TGCTCGTGTT CGGGCCCTGA GACGGGCACT GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514901.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514964.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X9, mRNA.

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CloneID	OMu45322
Clone ID Related Accession (Same CDS sequence)	XM_006514901.3
Accession Version	XM_006514901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCAAG CCTGGAAGTC CTGGTTGATA TACATGGTTT CTCGTAGGAC CAAACTTCAC 
ATGAAGACTA CTGCTCTAGA GTTTAGGAGG CAGAGTGTCT TATGTTTCTG GTGGAGCAAG 
TGGAGGTGGC GACTAGGACA AGCCCATGCA GAGCACGCTC TCCATGCAGT GGCTGTGAAG 
CACAGGGCCC TGAGCCTCCA GCTGCAGGGT TGGCTGCGAT GGCAAGAACA GCTTCTAATT 
AGCCAGCGAG ATAGGAGGAA GGAGGCCACT GCTGTGCAAC ACTATCAGCA CTGGCAGAAG 
CAGAGATCTC TGAAGGCTTG GCTTAAATAC CTCCAAATCT GCAGGGTGAA GAGATGGCAG 
AATGAGATGG CTGTACAATT CCACCGTGCT ACTGTGCTCC AGATACATTT CTGTGACTGG 
CAGTGGGCCT GGGAGTGGAG GCAGAGCTTG TCTGCCCACC AGGCCCTGGT GGTGAAGCTG 
GCCGGGAGGA TGGTCCTGCG GAGGGCCTTT ACACACTGGA AGCACTACAT GTTGCTACAG 
GCAGAAGAGG CTGCCCAGCG CGAGGCAGCA GCTGAACACC GCCAGCACTA CCTGCTGTAT 
TCTTGCTTTA GAGCCTTCAA AGACAACGTG ACCCAGGCCC GACTACAGCA AACAAGAAAG 
AAGCTTGCTC AGCAGCTGCG TGACACTACA TTGCTTCACA GGTTCTGGAA CCTCTGGCAG 
TCTCGAATTG AGCAGAGGGA GGAGAGGGTA CAGACCCCCT CATTGCATGC AGCCCTGAGC 
CACTACAGGG TGACAGTCCT ACACAAGTGT GTCAGGGTGT GGCTACGGTA TGTGCACAAG 
AGACAATGGC AGCAGTTATT GCGGGCCAGA GCAGATGGTC ATTTCCAGCA GCGAGCACTG 
CCTGCTGCCT TCTACACGTG GTACAGAGGC TGGCTATGGC ACCAGCAGAG ACGGATCCTG 
CACACAAAAG CAGTGCGTTT CCACAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514964.1
CDS	874..4122
Translation	MCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQAAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQGVLGSLAGAAGEPCHLDLNAARSSRKQPRRPSFLLERLGSQRSPEWYSLGEQQLEKPPEEESTALLGGSSLTRPFLPGVLPNVPGPKLPPTASPGLELLPPSSIMPHAAGGTARVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514901.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514901.3

ORF Insert Sequence:

ATGTGCCAAG CCTGGAAGTC CTGGTTGATA TACATGGTTT CTCGTAGGAC CAAACTTCAC 
ATGAAGACTA CTGCTCTAGA GTTTAGGAGG CAGAGTGTCT TATGTTTCTG GTGGAGCAAG 
TGGAGGTGGC GACTAGGACA AGCCCATGCA GAGCACGCTC TCCATGCAGT GGCTGTGAAG 
CACAGGGCCC TGAGCCTCCA GCTGCAGGGT TGGCTGCGAT GGCAAGAACA GCTTCTAATT 
AGCCAGCGAG ATAGGAGGAA GGAGGCCACT GCTGTGCAAC ACTATCAGCA CTGGCAGAAG 
CAGAGATCTC TGAAGGCTTG GCTTAAATAC CTCCAAATCT GCAGGGTGAA GAGATGGCAG 
AATGAGATGG CTGTACAATT CCACCGTGCT ACTGTGCTCC AGATACATTT CTGTGACTGG 
CAGTGGGCCT GGGAGTGGAG GCAGAGCTTG TCTGCCCACC AGGCCCTGGT GGTGAAGCTG 
GCCGGGAGGA TGGTCCTGCG GAGGGCCTTT ACACACTGGA AGCACTACAT GTTGCTACAG 
GCAGAAGAGG CTGCCCAGCG CGAGGCAGCA GCTGAACACC GCCAGCACTA CCTGCTGTAT 
TCTTGCTTTA GAGCCTTCAA AGACAACGTG ACCCAGGCCC GACTACAGCA AACAAGAAAG 
AAGCTTGCTC AGCAGCTGCG TGACACTACA TTGCTTCACA GGTTCTGGAA CCTCTGGCAG 
TCTCGAATTG AGCAGAGGGA GGAGAGGGTA CAGACCCCCT CATTGCATGC AGCCCTGAGC 
CACTACAGGG TGACAGTCCT ACACAAGTGT GTCAGGGTGT GGCTACGGTA TGTGCACAAG 
AGACAATGGC AGCAGTTATT GCGGGCCAGA GCAGATGGTC ATTTCCAGCA GCGAGCACTG 
CCTGCTGCCT TCTACACGTG GTACAGAGGC TGGCTATGGC ACCAGCAGAG ACGGATCCTG 
CACACAAAAG CAGTGCGTTT CCACAGAGGG ACATTAGAGA AGCAAGTATT TGCTCTCTGG 
AGGCAGAAGA TGTCTCAGCA TCGAGAAAAC TGCTTGGCAG AGAGAATGGC TATACTGCAG 
GCAGAGCAGC AGCTTTTGCG TAGGTTCTGG TTCGTGTGGC ACCAGCAGGC AGCAGTGTGT 
CAGCTGGAGC GACAGCAGCA AGCCATGGCC ATTGCCCACC ACCACAGTGG ACTGCTCAGG 
AGGGCTTTCT GCATCTGGAA GGAAAGCACC CAAGGATTTA GGATAGAGAG GATGGGCAGG 
GCACAGGCTG CGCACTTCCA CTCTGCACAG CTCTTGAGTC GGGCCTGGAG CATGTGGAGG 
GAGTGCCTGG CCCTGAGGCT GGAAGAGCAG CAGAAACTGA AGTGTGCGGC CCTGCACAGC 
CAGTGCATCC TGCTTCGCAG GGCTCTGCAG AAGTGGCTGG TTTACCAGAA TAGGGTGAGG 
AGTGTTCTGC GGGAGGTGGC GGCCAGGGAG AGACAGCACA ACAGGCAGCT GCTGTGGTGG 
GCGTTACATC TCTGGAGAGA AAACACCATG GCCCGTTTGG ATGGAGCCAA AAAAACCTCT 
CAAGCAAGAG TTCACTACAG CAGGACTCTG TGCTCCAAGG TCCTCGTCCA GTGGCGAGAG 
GTTACTTCAG TGCAGATATA TTACCGACAA AAGGAGGCAG CTGCCCTCAG GGAGGCCCGG 
AAAGCACTGG ACAGGGGTCG TCTCCAGAAC TGGTTTCAGC ACTGGCGATT CTGCAGCCAG 
AGGGCAGCCC AGCAGAGATT CCAGCTGGGA CAGGCGGCTC AGCATCACCA CTGGCAGCTG 
CTGATGGAAG CAATGGCCAG GTGGAAGGCA CACCATCTGG GGTGTATCAG AAAGAAGTTT 
CTGCAGAGGC AAGCTGCCCA ACTCCTGGCA CAGAGACTCA GTCGGGCCTG CTTCTGCCAG 
TGGAGGAAGC AGCTGGCAGT CCGGAAGCAA GAACAGTGGG GCACAGCCCG GGCCCTGTGG 
CTCTGGGCCT TCTCACTACA AGCAAAGGTG TGGACTGCAT GGCTGGGCTT CGTACTTGAG 
AGGAGGAGGA AGAAGGCACG GCTGGAACGG GCTATGCAAG CCTACCAGCA GCAGCTCCTA 
CAGGAGGGTG CCACACGGCT TCTGCGCTTC ACAGCTGGTA CGAAGGCCTT CCGGCAGCAG 
CTCCAGGCCC AGCAGCAGGT CCAGGCAGCC CACAGCCTCC ACTGTGCAGT CCGCCACTGT 
GCTGAGCTCT GGAAAAAGAA GGTGCTTGGT CCAGGCAAGA CTTCTCAACC ACCAGCACCC 
ACCACATTCA GCAAGAGAGT GACATTTAAG GATTCTTTCC TCTCTGGACA TGCTGCGGAG 
GCTGGAGATG CTACCCAGGA GACAAAGAAG CTTCGAGCTC CTCCATCTCA GGGAGTCCTG 
GGTAGTTTGG CAGGAGCTGC TGGGGAGCCC TGCCACCTAG ACCTCAATGC AGCCCGCTCA 
TCACGGAAAC AGCCCCGACG TCCATCCTTC CTGCTTGAGC GTTTGGGGAG TCAGAGGTCC 
CCAGAGTGGT ACAGCCTTGG GGAGCAGCAG TTAGAGAAGC CCCCAGAGGA GGAAAGCACG 
GCTCTGCTGG GAGGCTCTTC CCTGACAAGA CCCTTCCTAC CTGGGGTCCT GCCGAATGTT 
CCTGGTCCGA AGCTGCCTCC TACGGCCAGT CCAGGCCTGG AGCTGCTGCC TCCCTCCTCC 
ATCATGCCCC ATGCGGCAGG AGGCACTGCT AGGGTGTCAG CGAAGCCCAG CATCCCTGGG 
CCTCAGCCTT GGGGGTGCCC GTCCTTACCC AGGGACCTTG ATCCCCAACT CCTCCCTGGA 
GATTCCATAA GCACCCGGAC TGAGCCTGTT TATGGCTCAG AGGCTACAGG CCACACAGAG 
CTTGAGGCTG AGTTGGAGGG GATCCAGCAG CAGTTACAAC ACTACCAAAC CACCAAGCAG 
AACCTTTGGT CATGCCAGCG ACAAGCCAAC AGCCTGCGTA GGTGGCTGGA GCTAAGCCAG 
GAGGAGCCGA AATCCGAGGA CCTTCACCTA GAAGAGCAGG TGAAAACAGA ACTGGAGGAG 
GTAAGACCAG GTCAGCCCAG GGCCAGCCCC TGGCTGTCCT TCCTTTCAGC TTGCCTTGTC 
CCTCCCTCTC GCCCATGTCC CCAGGTGGAA CTACAGGTCC AGCAGCTGGC TAAGGAGCTT 
GAGGCCCAGC GTCAGCCTGT TGGTACATGC ATTGCTCGTG TTCGGGCCCT GAGACGGGCA 
CTGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514902.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514965.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu45323
Clone ID Related Accession (Same CDS sequence)	XM_006514902.4 , XM_006514902.3
Accession Version	XM_006514902.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTA 
AGACCAGGTC AGCCCAGGGC CAGCCCCTGG CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT 
CCCTCTCGCC CATGTCCCCA GGTGGAACTA CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG 
GCCCAGCGTC AGCCTGTTGG TACATGCATT GCTCGTGTTC GGGCCCTGAG ACGGGCACTG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514965.1
CDS	594..3779
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVRPGQPRASPWLSFLSACLVPPSRPCPQVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514902.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514902.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTA 
AGACCAGGTC AGCCCAGGGC CAGCCCCTGG CTGTCCTTCC TTTCAGCTTG CCTTGTCCCT 
CCCTCTCGCC CATGTCCCCA GGTGGAACTA CAGGTCCAGC AGCTGGCTAA GGAGCTTGAG 
GCCCAGCGTC AGCCTGTTGG TACATGCATT GCTCGTGTTC GGGCCCTGAG ACGGGCACTG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sfi1 ( XM_006514903.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006514966.1 Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu45324
Clone ID Related Accession (Same CDS sequence)	XM_006514903.3 , XM_006514903.4
Accession Version	XM_006514903.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein SFI1 homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTG 
GAACTACAGG TCCAGCAGCT GGCTAAGGAG CTTGAGGCCC AGCGTCAGCC TGTTGGTACA 
TGCATTGCTC GTGTTCGGGC CCTGAGACGG GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006514966.1
CDS	595..3696
Translation	MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARKFLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTFVHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLCFWWSKWRWRLGQAHAEHALHAVAVKHRALSLQLQGWLRWQEQLLISQRDRRKEATAVQHYQHWQKQRSLKAWLKYLQICRVKRWQNEMAVQFHRATVLQIHFCDWQWAWEWRQSLSAHQALVVKLAGRMVLRRAFTHWKHYMLLQAEEAAQREAAAEHRQHYLLYSCFRAFKDNVTQARLQQTRKKLAQQLRDTTLLHRFWNLWQSRIEQREERVQTPSLHAALSHYRVTVLHKCVRVWLRYVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQHRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIERMGRAQAAHFHSAQLLSRAWSMWRECLALRLEEQQKLKCAALHSQCILLRRALQKWLVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKKTSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQLGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARALWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQVSAKPSIPGPQPWGCPSLPRDLDPQLLPGDSISTRTEPVYGSEATGHTELEAELEGIQQQLQHYQTTKQNLWSCQRQANSLRRWLELSQEEPKSEDLHLEEQVKTELEEVELQVQQLAKELEAQRQPVGTCIARVRALRRALC

Target ORF information:

RefSeq Version	XM_006514903.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Sfi1 homolog, spindle assembly associated (yeast) (Sfi1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514903.3

ORF Insert Sequence:

ATGGAGAAGA AGATTGGTTC CAGGTCTTTT AGGGATGGTG TGGTTAAGAA ACCTTGTTCT 
CCAAAGACCC TGCCCCTCAA AAAGTCTTCT GCCTTCTCTG GGATCCAGAG GGAGCCATCT 
AGAAGCTGCC ACTCTATATA CTATCATGCC TCACAAAACT GGACTCGGTA CCGATTACAA 
GAACTTCGAA TCAGGTGTGT GGCCAGAAAG TTCCTGTATT TGTGGATTCG AGTGACTTTT 
GGAAGAGTGA CTCCCTCTAG AGCCAGGATT TTCCATGAGC AGAAAATATT ACAGAAGGTC 
TTCGGAGAAT GGAGGGAAGA ATGGTGGGTC TCCCAGAGGG AGTGGAAGCT CTGTGTCCGA 
GCTGACTGTC ACTACAGATA CTACTTGTAC AACCTGATTT TCCAGAACTG GAAGACCTTT 
GTGCACCAGC AACGGGAGAT GAGGAAGAGG TTCCGAATAG CTGAGCATCA TGATACGAAG 
CAGAAGATGT GCCAAGCCTG GAAGTCCTGG TTGATATACA TGGTTTCTCG TAGGACCAAA 
CTTCACATGA AGACTACTGC TCTAGAGTTT AGGAGGCAGA GTGTCTTATG TTTCTGGTGG 
AGCAAGTGGA GGTGGCGACT AGGACAAGCC CATGCAGAGC ACGCTCTCCA TGCAGTGGCT 
GTGAAGCACA GGGCCCTGAG CCTCCAGCTG CAGGGTTGGC TGCGATGGCA AGAACAGCTT 
CTAATTAGCC AGCGAGATAG GAGGAAGGAG GCCACTGCTG TGCAACACTA TCAGCACTGG 
CAGAAGCAGA GATCTCTGAA GGCTTGGCTT AAATACCTCC AAATCTGCAG GGTGAAGAGA 
TGGCAGAATG AGATGGCTGT ACAATTCCAC CGTGCTACTG TGCTCCAGAT ACATTTCTGT 
GACTGGCAGT GGGCCTGGGA GTGGAGGCAG AGCTTGTCTG CCCACCAGGC CCTGGTGGTG 
AAGCTGGCCG GGAGGATGGT CCTGCGGAGG GCCTTTACAC ACTGGAAGCA CTACATGTTG 
CTACAGGCAG AAGAGGCTGC CCAGCGCGAG GCAGCAGCTG AACACCGCCA GCACTACCTG 
CTGTATTCTT GCTTTAGAGC CTTCAAAGAC AACGTGACCC AGGCCCGACT ACAGCAAACA 
AGAAAGAAGC TTGCTCAGCA GCTGCGTGAC ACTACATTGC TTCACAGGTT CTGGAACCTC 
TGGCAGTCTC GAATTGAGCA GAGGGAGGAG AGGGTACAGA CCCCCTCATT GCATGCAGCC 
CTGAGCCACT ACAGGGTGAC AGTCCTACAC AAGTGTGTCA GGGTGTGGCT ACGGTATGTG 
CACAAGAGAC AATGGCAGCA GTTATTGCGG GCCAGAGCAG ATGGTCATTT CCAGCAGCGA 
GCACTGCCTG CTGCCTTCTA CACGTGGTAC AGAGGCTGGC TATGGCACCA GCAGAGACGG 
ATCCTGCACA CAAAAGCAGT GCGTTTCCAC AGAGGGACAT TAGAGAAGCA AGTATTTGCT 
CTCTGGAGGC AGAAGATGTC TCAGCATCGA GAAAACTGCT TGGCAGAGAG AATGGCTATA 
CTGCAGGCAG AGCAGCAGCT TTTGCGTAGG TTCTGGTTCG TGTGGCACCA GCAGGCAGCA 
GTGTGTCAGC TGGAGCGACA GCAGCAAGCC ATGGCCATTG CCCACCACCA CAGTGGACTG 
CTCAGGAGGG CTTTCTGCAT CTGGAAGGAA AGCACCCAAG GATTTAGGAT AGAGAGGATG 
GGCAGGGCAC AGGCTGCGCA CTTCCACTCT GCACAGCTCT TGAGTCGGGC CTGGAGCATG 
TGGAGGGAGT GCCTGGCCCT GAGGCTGGAA GAGCAGCAGA AACTGAAGTG TGCGGCCCTG 
CACAGCCAGT GCATCCTGCT TCGCAGGGCT CTGCAGAAGT GGCTGGTTTA CCAGAATAGG 
GTGAGGAGTG TTCTGCGGGA GGTGGCGGCC AGGGAGAGAC AGCACAACAG GCAGCTGCTG 
TGGTGGGCGT TACATCTCTG GAGAGAAAAC ACCATGGCCC GTTTGGATGG AGCCAAAAAA 
ACCTCTCAAG CAAGAGTTCA CTACAGCAGG ACTCTGTGCT CCAAGGTCCT CGTCCAGTGG 
CGAGAGGTTA CTTCAGTGCA GATATATTAC CGACAAAAGG AGGCAGCTGC CCTCAGGGAG 
GCCCGGAAAG CACTGGACAG GGGTCGTCTC CAGAACTGGT TTCAGCACTG GCGATTCTGC 
AGCCAGAGGG CAGCCCAGCA GAGATTCCAG CTGGGACAGG CGGCTCAGCA TCACCACTGG 
CAGCTGCTGA TGGAAGCAAT GGCCAGGTGG AAGGCACACC ATCTGGGGTG TATCAGAAAG 
AAGTTTCTGC AGAGGCAAGC TGCCCAACTC CTGGCACAGA GACTCAGTCG GGCCTGCTTC 
TGCCAGTGGA GGAAGCAGCT GGCAGTCCGG AAGCAAGAAC AGTGGGGCAC AGCCCGGGCC 
CTGTGGCTCT GGGCCTTCTC ACTACAAGCA AAGGTGTGGA CTGCATGGCT GGGCTTCGTA 
CTTGAGAGGA GGAGGAAGAA GGCACGGCTG GAACGGGCTA TGCAAGCCTA CCAGCAGCAG 
CTCCTACAGG AGGGTGCCAC ACGGCTTCTG CGCTTCACAG CTGGTACGAA GGCCTTCCGG 
CAGCAGCTCC AGGCCCAGCA GCAGGTCCAG GTGTCAGCGA AGCCCAGCAT CCCTGGGCCT 
CAGCCTTGGG GGTGCCCGTC CTTACCCAGG GACCTTGATC CCCAACTCCT CCCTGGAGAT 
TCCATAAGCA CCCGGACTGA GCCTGTTTAT GGCTCAGAGG CTACAGGCCA CACAGAGCTT 
GAGGCTGAGT TGGAGGGGAT CCAGCAGCAG TTACAACACT ACCAAACCAC CAAGCAGAAC 
CTTTGGTCAT GCCAGCGACA AGCCAACAGC CTGCGTAGGT GGCTGGAGCT AAGCCAGGAG 
GAGCCGAAAT CCGAGGACCT TCACCTAGAA GAGCAGGTGA AAACAGAACT GGAGGAGGTG 
GAACTACAGG TCCAGCAGCT GGCTAAGGAG CTTGAGGCCC AGCGTCAGCC TGTTGGTACA 
TGCATTGCTC GTGTTCGGGC CCTGAGACGG GCACTGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread and dynamic translational control of red blood cell development.
Blood129(5)619-629(2017 02)
Alvarez-Dominguez JR,Zhang X,Hu W

Divergence patterns of genic copy number variation in natural populations of the house mouse (Mus musculus domesticus) reveal three conserved genes with major population-specific expansions.
Genome research25(8)1114-24(2015 Aug)
Pezer ?,Harr B,Teschke M,Babiker H,Tautz D

Global discovery of erythroid long noncoding RNAs reveals novel regulators of red cell maturation.
Blood123(4)570-81(2014 Jan)
Alvarez-Dominguez JR,Hu W,Yuan B,Shi J,Park SS,Gromatzky AA,van Oudenaarden A,Lodish HF

The Vertebrate Genome Annotation browser 10 years on.
Nucleic acids research42(Database issue)D771-9(2014 Jan)
Harrow JL,Steward CA,Frankish A,Gilbert JG,Gonzalez JM,Loveland JE,Mudge J,Sheppard D,Thomas M,Trevanion S,Wilming LG

Genome-wide analysis of H4K5 acetylation associated with fear memory in mice.
BMC genomics14539(2013 Aug)
Park CS,Rehrauer H,Mansuy IM

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Sfi1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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