atrx cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	atrx
Entrez Gene ID	100380046
Full Name	alpha thalassemia/mental retardation syndrome X-linked
Synonyms	rad54
General protein information	Preferred Names alpha thalassemia/mental retardation syndrome X-linked Names transcriptional regulator ATRX alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931812.4	XP_002931858.1	transcriptional regulator ATRX isoform X1
XM_004915742.3	XP_004915799.1	transcriptional regulator ATRX isoform X3
XM_002931812.5	XP_002931858.1	transcriptional regulator ATRX isoform X1
XM_004915742.4	XP_004915799.1	transcriptional regulator ATRX isoform X2
XM_004915741.3	XP_004915798.1	transcriptional regulator ATRX isoform X2

Pathways from BioSystems

Homologies

	ATRX	XP_001099874.1
Bos taurus (cattle)	ATRX	XP_005228030.1
Mus musculus (house mouse)	Atrx	NP_033556.2
Gallus gallus (chicken)	ATRX	XP_420305.3
Homo sapiens (human)	ATRX	NP_000480.3
Canis lupus familiaris (dog)	ATRX	XP_538084.2
Danio rerio (zebrafish)	atrx	NP_956947.2
Xenopus tropicalis (tropical clawed frog)	atrx	XP_002931858.1
Pan troglodytes (chimpanzee)	ATRX	NP_001009018.1
Rattus norvegicus (Norway rat)	Atrx	XP_006257219.1

Gene Ontology

The following atrx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atrx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa65475	XM_002931812.5 Latest version!	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa65476	XM_004915742.4 Latest version!	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa12505	XM_002931812.4 Latest version!	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa12506	XM_004915741.3 Latest version!	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa12507	XM_004915742.3 Latest version!	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atrx ( XM_002931812.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931858.1 Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa65475
Clone ID Related Accession (Same CDS sequence)	XM_002931812.5
Accession Version	XM_002931812.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional regulator ATRX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931812.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCGG AGCCCATGAG TGAAAGCAAG TTAAATACAT TGGTGAAGAA ACTTCATGAT 
TTTCTAGCTC ACTCCTCAGA AGAATCTGAG GACACAACTT CTCCAACAAG ACAGTCTGTG 
AGCAGAAGTG CAGCTAAAGT CAAGTCTGTA CACCGACTGC ATCCAATGGA AAACAACATG 
GATGAGGGAA GTACTTCTGA AAAACTCAAG TCCTTGGGAT CATCTCGTTC AAAAAGGAAA 
CCCTCATTTG TCCAAAAATA TGTTGGGTCA GATGAAGAGA CTGTGCCAGA TGAAGTTGTC 
AATTTAGATG GGTCTAATGA GAATTCTGAG AATGAAGTTG ATATGGAAAG CTTGCCCATA 
GGTACAGTGG TTGTACAACC AGAACCTGTC TTAAACGAAG ATAAAGATGA TTTCAAAGGA 
CCAGAGTTTC GAAGCAGAAA CAAACCTAAA CCAGATATTA CTATACCTAA GAAACGCACA 
GAGGATGGAC TTCATGGTAT TGTTAACTGC ACAGCCTGTG GCCAACAAGT AAATCATTTT 
CAGAAGGATT CAATTTATCG ACATCCTGCT TTAAAAGTGC TGATATGTAA GGCATGCTAT 
AAATATTACA TGAGTGATGA CATAAACCGG GATGCAGACG GAATGGATGA ACAGTGCAGA 
TGGTGTGCTG AAGGAGGAAA CTTGATTTGC TGTGATTACT GTCATAATGC CTTTTGTAAG 
AAGTGCATTC TACGAAATCT AGGGAGAAAG GAACTGTCTG CTATTATGGA TGAAGAAATG 
AAGTGGGAGT GCTACATTTG CCGACCAGAA CCTTTACTGG ACTCTGTAAC TGCATGTGAC 
AGTGTGTTTG AAAATTTAGA GCAATTGTTA CAACAAAACA AGAAGAAAAT GAAATCAGAC 
AATGAAAAAA ACAAATCAAA TGAGCACTCC CAAAATGATT CTGATAAAAG GAATTCAAGC 
TACAATGGAC AGGGCAAAAA TGTAGGTGAT GCACATGCCG GTACTTTAAC GTATTCTTAT 
AGAGCCCTTA TGGTGCCAAA GGAGATGCTC AAAAAAGCGA AAAAGCTTAT TGAGACAACA 
ACTAGCCTGA ATGCAAGTTT TGTAAAGTTT ATGAAACAAG GAACTGCGAA CAATGAATTG 
AACCCTGCTG AAAAGTTAAG ACAGCTGAAA GCATTTAAAT CTGTCCTGGG TGACTTAAAG 
AAAGCCCATG AGGCTTTGGA GGAAACATTA ATGGAAGAGA TTCATTCAGT TGACGAGCAA 
AAGAAATCTA AGAAAAGCAA AGAGACTACG AGTGAGAATA AACATGCAAA GGAGAAGAAA 
CTTGGTGAAG AAAAGCAGAA AGAACCCAAG AAAATAGTTG CACGTGAAAA AGCAAAACCT 
TCAGATTCTG ACAATAAGCA AAGGGATTGT GACAAGAATG CCTCTAACGA TACAGAGCAG 
GCTGAAGAAG CGTTGATACA GAATGGGAAA AATGTAGATC TTACTGAAGA AGATACAATA 
AAGAGTGAAC CCTGTTCAGC TGCTGCTGAC GTTACAGAGG TTTTGGCAAC AGATGCAGAG 
GGTACAGATA TAAGTGTCGT CATGGAGGAG GCAGATGAAA ATGTTGATGG AAAAAATGAA 
AAAGCAACAG TTGAGAGTGA TGATAATGAA GGTAAGCAAA AATCCCAGAA AAAGAAGCAG 
AAGAGTATGC ATGACAAACC TAGGGAAAAC TCACCTGGTA ACAGAAAGAA GAGTGTAGAT 
TCACCTAAGA AAGAACGGGA GTCTGTGCTT CCTGAAAAGA CTAAAGAGAA GAAAAGGGCT 
GATCAGTGCA CTGAGTCTTC TAAATCTGGG AAGTCGCGCA CACCACAAAA GAAATCCAAA 
AATGCAGAGG CGACATTTGA GCCCAATTCC ACTGAAGCTC TTGATATGGA CATTGTTTCA 
GTCCCATCTT CTGTGCCAGA GGATATATTT GATAGCTTGG ATGCATCATT AGAGCAGCAA 
AATTCTTTGC AAGATTCTGT AACGAGTACT GCAGTATCTA CACCAAAGTG CAAGGAAGAA 
CTAAAGCCAC TGGTCAAGAA GCAGTTGATT GTGCAGCTGA CACCAGTGTC TCTATCCAAT 
TCCCCAGTGA AAGCAGACAG CAATAGTGCA GGCAAATCGC CAAAGGCTGA CATTGGTAGT 
GATGCTGAAA CGGAGGCGCA GAATCAAAAT AGTGCTTCAT GTGATGAAGT CCTTGAGAAC 
TCATCGGAGA ACGATCTAGA GGAATCTGCT CTCAGGAGAT CACCTCGCGT AAAAACTACT 
CCAAGGAGGC GACAAACTGA TGCAAATTCT GCAGCCTCAA ATACAGAAGA AGAGAGCAAT 
GACACAGACA AAGAGAAAAA AAGCAAAAGG GCAGCATGTT CTAAAGAAAA ACGGAATTCT 
ACAGCAAAGC ATAGCCCTGC AGAAAGTGGG GAGAGCCCCA AAGCAAAAAG ATCATTAGCA 
AAAAAGATGC AGGCACAAGT GAATAAGAGT ACTTCTGATT CAGATGAGGT TCCAGAAGTA 
CTTAAAAAGG CAGCCCTTAT GAGTAACAGC TCCTCAGAGC TTGATAGCAG TAATGAGGCA 
AACAGTGGTA AAAGGACTTC TAATCGTCAA GATAAAACAG CAAAAGGTTA TTCTAAGAGA 
AAAAGAAGTA GCTCAAGTTC TGGTTCTGAG CCAGCCAAAA GGACAACTAG AAACTCAACA 
GCTGCCAAAA AGAAACGTGG GGACCAATCG GACACTTCAA ATTCTGAGCT GGAGAAGGAA 
GTAAAAAAAC TCGGTAAAAA AGATTCTGTC AGGAAGGGCT TAAAGCAGAA ATCTAAGAAA 
AAGGAAGAAA ACAGTGAGTC CTCTGATGAA AAGAATCAAA ATAGATCCCA GAAAGCCAAA 
AAATCCAAAA GAGTGAGAAC ACCTGCCTCT AATAAGAAGG AATCCTCATC CGACCTTTCT 
GAAGAAGAGC AAGAAAATAG CCACAATTCA GATGAAGACA GCGATGAACA AAAGATCAAA 
CCATTAGTGG AAAATGAAGT TGTATCTGCC ACAGGATTTG TTCAATCATC AGGTGATGAA 
GCAGGTACAA AATGTGGGGC AGCTCCTACT GAGGAGGATG ATGACGATGA TGATGACCCT 
GAGAATAGGA TTGCCAAGAA AATGCTTCTG GAAGAAATCA AAGCCAATCT GTCTTCAGAT 
GATGTGGAAG AAGCATCCTC TGATGAAGAG CCAGAAGAAG GCAAAAAATC TGTGGCAAAG 
TCAACTACAA AAACTGAAGA TAAGGATGTT AATGAGGATG ATAAGTCCGA TTCAGATTCA 
GACTCTGACT CTGACTCTGA TTCAGAGTCT GAGAAAAAAC CAAGATATAG ACACCACCTT 
TTGCGCCGCA AGATAAGTTT AAGCGATGGA GATTCTGGAG AAGAAAAAGA GAAAAAAGAC 
AAAGCTAAGA ACTCAAAGAA CGGAAAGCGT CGAAACAGAC GGAAAGTGAG CAGTGAGGAA 
TCGGCCTCTG ACTTCCATAA ATCATCTGGG GTTAGCGAGG AAATTTCAGA GAGTGATGAT 
TCAGATGATC AGCGGCCCAG AACAAGAGCT GCAAAGAAAG AAGCCGAAGA GAACCAGCGT 
AGCTACAAGC AGAAAAAGAA GAGAAGGCGA ATTAAAGTCC AAGATGACTC CAGTGATAGC 
AAGAGTAATA AGGGTAATTC AGAGGATGGA GACCATGATG AGGACTCAGA CTCCAAATCA 
CCTGGTAAGG GCAGGAAGAA GATTAGGAAG ATTATTAAAG ATGACAAGTT GCGAACAGAA 
ACACAGAATG CCTTGAAAGA GGAAGAGGAG AGAAGGAAAA GAATAGCCGA GAGAGAGCGA 
GAACGAGACA AACTAAGAGA GTTACAGGTC GTTGATACTG AAGATGTCTC GCCTGTAAAG 
TGTCCAGTCA CTACTAAACT AGTGTTGGAT GAAAATGAAG AAACAAAAGA GGCTTTGGTG 
CAGGTTCACC AAAAGATGGT TACAAAGTTG AAACCGCATC AGGTAGACGG TGTTCAGTTC 
ATGTGGGACT GTTGCTGTGA GTCTATGAAA AAGACTAAAA AGGACCCTGG TTCTGGCTGT 
ATATTGGCTC ATTGTATGGG TCTTGGCAAG ACTTTACAGG TTGTGACTTT CCTGCACACT 
GTTCTTCTGT CTGAGAAGCT GGATTTCAGC ACTGCTCTTG TGGTGTGTCC TTTAAATACT 
GTTTTAAATT GGCTCAATGA ATTTGAGAAG TGGCAGGATG GCATGGACGA TGAAGAGAAA 
TTGGAGGTCT GTGAACTTGC TACAGTGAAG CGTCCTCAGG AAAGGTCATA CATGATGCAA 
CGCTGGCATC AAGAAGGAGG AATCTTAATA ATTGGATATG AAATGTACCG AAACCTTACT 
CAAGGGAGGA ATGTAAAAAG TAAAAAGTTG AAAGAGATTT TTCACAAGAC CCTTGTGGAT 
CCAGGACCTG ACTTTGTGAT ATGTGATGAA GGACACATCC TCAAAAATGA ATTGTCAGCC 
ATTTCTAAGG CCATGAATTC CATAAAGACA AGAAGAAGAA TCATACTTAC AGGAACCCCA 
CTGCAAAATA ATCTTGTAGA ATATCACTGC ATGGTCAACT TCATCAAGGA AAACCTACTT 
GGTTCCATTA AGGAATTCAG AAATCGTTTC ATCAATCCCA TCCAGAACGG ACAGTGCGCC 
GACTCTACCC TAGTTGATGT CAGAGTGATG AAAAAGAGAG CTCACATCTT GTATGAAATG 
TTAGCTGGCT GTGTTCAGCG AAGAGATTAT ACTGCACTAA CAAAGTTCTT GCCACCAAAG 
CATGAGTATG TATTGTCTGT CAGAATTTCC CCACTTCAGT GTAAGCTTTA CCAATACTAT 
CTGGACCACT TAACAGGAGC TGTTGGTGAA GGAGGTGTAA GAGGAAAATC TGGAACAAAA 
CTTTTCCAGG ATTTCCAGGT CTTGAGCAGA ATCTGGACTC ACCCTTGGTG TTTGCAGCTG 
GACTATATTA GTAAAGAAAA TAAGGGTTAC TTTGATGAAG ACGACAGCAT GGAAGAGTTC 
ATAGCTTCCG ACTCTGAAGA ATCCTCCATG AGTCTGAGTT CTGAGGAAGA AGTTGTTAAT 
AAAAAGAAGG CCAAGGGAAA AGGCAAACGG GAGAACAGCT CAAGTGAAGA AGAAAGTGAC 
AATGATGTTG AAGTTATTAA GGTGTGGAAT TCAAGATCTC GAGGCGGCAA AGGAAAAGAT 
CTGAATGATG AAGAGGCCTC AAACAGCCCT GTAGTTCCTA AAGCTGAAGA AACAAAAGCA 
ACATCCTCTT CCACACCTGG AAGCCCAGCA GCTGACTGGT ACAAGGATTT TGTTACTGAA 
GCAGATTCAT CTATCATGGA GCATTCTGGA AAAATAATGC TTTTGTTTGA GATTTTGCGC 
ATGGCTGAGG TGTTGGGTGA CAAAGTTTTA GTTTTCAGCC AGTCTCTCAT ATCTCTGGAT 
TTGATAGAAG ATTTTCTGGA ATTGGCAAGT ACAGAGAAAA ATGAAGAAAA TGCATCTGAA 
AAGCCAGTAT TTTACAAAGG GGATGGGAAG TGGTTTAGAA ATATCGATTA CTATCGTTTA 
GACGGCCAAA CCTCCCCTCA AACACGTAAA AAGTGGGCAG AGGAGTTCAA TGATTCGACC 
AATATAAGGG GCCGTCTGTT CCTTATTTCT ACAAAAGCTG GATCCCTGGG TATTAATCTT 
GTTGCTGCTA ATCGTGTGAT AATCTTTGAT GCATCATGGA ATCCATCCTA TGATATCCAG 
AGTATCTTCA GGGTGTATCG ATTTGGACAG ATAAAGCCTG TATTTGTCTA CAGGTTTTTA 
GCCCAGGGCA CCATGGAAGA AAAGATATAT GACAGGCAGA TCGCCAAACA GTCCCTGTCC 
TTTAGAGTTA TTGATCAACA ACAAATAGAA CGTCACTTTA CAATGAATGA ACTGACTGAA 
CTTTACACCT TTGAGCCTGA TCTGCTGGAT GACCCTAATT CCGAAAAGAA GAAAAAGCGA 
GAGACTCCCA TGCTGCCTAA GGATACTGTG CTAGCAGAGC TCTTACAAAT CTTGAAGGAG 
TACATAGTCA CTTATCATGA GCACGACTCT CTTCTGGATC ATAAGGAGGA AGAGGCCCTT 
ACAGAAGAGG ATCGGAAGGC AGCATGGGCA GAATATGAAG CTGAAAAGAA GGGTTTGACC 
AATCGTTTTA ACGTGGCAGC AGTAACTGGG GCCAACTACG CTCAAGTCAA TATAAACTCG 
CAATCTTCAT ACATGCCTTT AAACTTGGGA GCGCTGTCTT CTTTATCCAA TCAGCAACTC 
GAGGATTTAA TAAATCAAGG CAGAGAAAAA GTGGTTGAAG CTACAAATAA CATAGCAACT 
GCAAGGATTC AACCCCTTGA AGACATTATC TCGACAGTGT GGAAGGAAAA TCCGACTGCC 
ACGGAGGCCC AAGTTCAGTC CCTTGCCCTA AGCAGGCAGT CGAGCCAAGA ATTGGAAATA 
AAGAGACGTG AGGCCATTTA TAACGACGTA CTGACTAAGC AACAGATGTT GATCAGCTGC 
GTGCAGCGAA TTCTCATGAG TCGAAGGTTA CAGCACCAAT ACAACCAGCA ACAGCAGCAG 
CAACAAATTG TTTTTCAACA GCAAGCAGCT CTGAATCGCC TGATGATGCA GAAAAATCCA 
AATATTATAA TATCCCCTTC TAACTTTTCT CAAATTGACA TGAGAGGAAT GTACCAAGCT 
GGGATGCAAG GTCATCAGAT GCAGCGTAAT GCACCCGCCT CTATAAGAGC AAAAAATCCA 
GGACCTTCCC AGGGAAAAAA AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931858.1
CDS	462..7268
Translation	MTAEPMSESKLNTLVKKLHDFLAHSSEESEDTTSPTRQSVSRSAAKVKSVHRLHPMENNMDEGSTSEKLKSLGSSRSKRKPSFVQKYVGSDEETVPDEVVNLDGSNENSENEVDMESLPIGTVVVQPEPVLNEDKDDFKGPEFRSRNKPKPDITIPKKRTEDGLHGIVNCTACGQQVNHFQKDSIYRHPALKVLICKACYKYYMSDDINRDADGMDEQCRWCAEGGNLICCDYCHNAFCKKCILRNLGRKELSAIMDEEMKWECYICRPEPLLDSVTACDSVFENLEQLLQQNKKKMKSDNEKNKSNEHSQNDSDKRNSSYNGQGKNVGDAHAGTLTYSYRALMVPKEMLKKAKKLIETTTSLNASFVKFMKQGTANNELNPAEKLRQLKAFKSVLGDLKKAHEALEETLMEEIHSVDEQKKSKKSKETTSENKHAKEKKLGEEKQKEPKKIVAREKAKPSDSDNKQRDCDKNASNDTEQAEEALIQNGKNVDLTEEDTIKSEPCSAAADVTEVLATDAEGTDISVVMEEADENVDGKNEKATVESDDNEGKQKSQKKKQKSMHDKPRENSPGNRKKSVDSPKKERESVLPEKTKEKKRADQCTESSKSGKSRTPQKKSKNAEATFEPNSTEALDMDIVSVPSSVPEDIFDSLDASLEQQNSLQDSVTSTAVSTPKCKEELKPLVKKQLIVQLTPVSLSNSPVKADSNSAGKSPKADIGSDAETEAQNQNSASCDEVLENSSENDLEESALRRSPRVKTTPRRRQTDANSAASNTEEESNDTDKEKKSKRAACSKEKRNSTAKHSPAESGESPKAKRSLAKKMQAQVNKSTSDSDEVPEVLKKAALMSNSSSELDSSNEANSGKRTSNRQDKTAKGYSKRKRSSSSSGSEPAKRTTRNSTAAKKKRGDQSDTSNSELEKEVKKLGKKDSVRKGLKQKSKKKEENSESSDEKNQNRSQKAKKSKRVRTPASNKKESSSDLSEEEQENSHNSDEDSDEQKIKPLVENEVVSATGFVQSSGDEAGTKCGAAPTEEDDDDDDDPENRIAKKMLLEEIKANLSSDDVEEASSDEEPEEGKKSVAKSTTKTEDKDVNEDDKSDSDSDSDSDSDSESEKKPRYRHHLLRRKISLSDGDSGEEKEKKDKAKNSKNGKRRNRRKVSSEESASDFHKSSGVSEEISESDDSDDQRPRTRAAKKEAEENQRSYKQKKKRRRIKVQDDSSDSKSNKGNSEDGDHDEDSDSKSPGKGRKKIRKIIKDDKLRTETQNALKEEEERRKRIAERERERDKLRELQVVDTEDVSPVKCPVTTKLVLDENEETKEALVQVHQKMVTKLKPHQVDGVQFMWDCCCESMKKTKKDPGSGCILAHCMGLGKTLQVVTFLHTVLLSEKLDFSTALVVCPLNTVLNWLNEFEKWQDGMDDEEKLEVCELATVKRPQERSYMMQRWHQEGGILIIGYEMYRNLTQGRNVKSKKLKEIFHKTLVDPGPDFVICDEGHILKNELSAISKAMNSIKTRRRIILTGTPLQNNLVEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQCADSTLVDVRVMKKRAHILYEMLAGCVQRRDYTALTKFLPPKHEYVLSVRISPLQCKLYQYYLDHLTGAVGEGGVRGKSGTKLFQDFQVLSRIWTHPWCLQLDYISKENKGYFDEDDSMEEFIASDSEESSMSLSSEEEVVNKKKAKGKGKRENSSSEEESDNDVEVIKVWNSRSRGGKGKDLNDEEASNSPVVPKAEETKATSSSTPGSPAADWYKDFVTEADSSIMEHSGKIMLLFEILRMAEVLGDKVLVFSQSLISLDLIEDFLELASTEKNEENASEKPVFYKGDGKWFRNIDYYRLDGQTSPQTRKKWAEEFNDSTNIRGRLFLISTKAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQIKPVFVYRFLAQGTMEEKIYDRQIAKQSLSFRVIDQQQIERHFTMNELTELYTFEPDLLDDPNSEKKKKRETPMLPKDTVLAELLQILKEYIVTYHEHDSLLDHKEEEALTEEDRKAAWAEYEAEKKGLTNRFNVAAVTGANYAQVNINSQSSYMPLNLGALSSLSNQQLEDLINQGREKVVEATNNIATARIQPLEDIISTVWKENPTATEAQVQSLALSRQSSQELEIKRREAIYNDVLTKQQMLISCVQRILMSRRLQHQYNQQQQQQQIVFQQQAALNRLMMQKNPNIIISPSNFSQIDMRGMYQAGMQGHQMQRNAPASIRAKNPGPSQGKKM

Target ORF information:

RefSeq Version	XM_002931812.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931812.5

ORF Insert Sequence:

ATGACAGCGG AGCCCATGAG TGAAAGCAAG TTAAATACAT TGGTGAAGAA ACTTCATGAT 
TTTCTAGCTC ACTCCTCAGA AGAATCTGAG GACACAACTT CTCCAACAAG ACAGTCTGTG 
AGCAGAAGTG CAGCTAAAGT CAAGTCTGTA CACCGACTGC ATCCAATGGA AAACAACATG 
GATGAGGGAA GTACTTCTGA AAAACTCAAG TCCTTGGGAT CATCTCGTTC AAAAAGGAAA 
CCCTCATTTG TCCAAAAATA TGTTGGGTCA GATGAAGAGA CTGTGCCAGA TGAAGTTGTC 
AATTTAGATG GGTCTAATGA GAATTCTGAG AATGAAGTTG ATATGGAAAG CTTGCCCATA 
GGTACAGTGG TTGTACAACC AGAACCTGTC TTAAACGAAG ATAAAGATGA TTTCAAAGGA 
CCAGAGTTTC GAAGCAGAAA CAAACCTAAA CCAGATATTA CTATACCTAA GAAACGCACA 
GAGGATGGAC TTCATGGTAT TGTTAACTGC ACAGCCTGTG GCCAACAAGT AAATCATTTT 
CAGAAGGATT CAATTTATCG ACATCCTGCT TTAAAAGTGC TGATATGTAA GGCATGCTAT 
AAATATTACA TGAGTGATGA CATAAACCGG GATGCAGACG GAATGGATGA ACAGTGCAGA 
TGGTGTGCTG AAGGAGGAAA CTTGATTTGC TGTGATTACT GTCATAATGC CTTTTGTAAG 
AAGTGCATTC TACGAAATCT AGGGAGAAAG GAACTGTCTG CTATTATGGA TGAAGAAATG 
AAGTGGGAGT GCTACATTTG CCGACCAGAA CCTTTACTGG ACTCTGTAAC TGCATGTGAC 
AGTGTGTTTG AAAATTTAGA GCAATTGTTA CAACAAAACA AGAAGAAAAT GAAATCAGAC 
AATGAAAAAA ACAAATCAAA TGAGCACTCC CAAAATGATT CTGATAAAAG GAATTCAAGC 
TACAATGGAC AGGGCAAAAA TGTAGGTGAT GCACATGCCG GTACTTTAAC GTATTCTTAT 
AGAGCCCTTA TGGTGCCAAA GGAGATGCTC AAAAAAGCGA AAAAGCTTAT TGAGACAACA 
ACTAGCCTGA ATGCAAGTTT TGTAAAGTTT ATGAAACAAG GAACTGCGAA CAATGAATTG 
AACCCTGCTG AAAAGTTAAG ACAGCTGAAA GCATTTAAAT CTGTCCTGGG TGACTTAAAG 
AAAGCCCATG AGGCTTTGGA GGAAACATTA ATGGAAGAGA TTCATTCAGT TGACGAGCAA 
AAGAAATCTA AGAAAAGCAA AGAGACTACG AGTGAGAATA AACATGCAAA GGAGAAGAAA 
CTTGGTGAAG AAAAGCAGAA AGAACCCAAG AAAATAGTTG CACGTGAAAA AGCAAAACCT 
TCAGATTCTG ACAATAAGCA AAGGGATTGT GACAAGAATG CCTCTAACGA TACAGAGCAG 
GCTGAAGAAG CGTTGATACA GAATGGGAAA AATGTAGATC TTACTGAAGA AGATACAATA 
AAGAGTGAAC CCTGTTCAGC TGCTGCTGAC GTTACAGAGG TTTTGGCAAC AGATGCAGAG 
GGTACAGATA TAAGTGTCGT CATGGAGGAG GCAGATGAAA ATGTTGATGG AAAAAATGAA 
AAAGCAACAG TTGAGAGTGA TGATAATGAA GGTAAGCAAA AATCCCAGAA AAAGAAGCAG 
AAGAGTATGC ATGACAAACC TAGGGAAAAC TCACCTGGTA ACAGAAAGAA GAGTGTAGAT 
TCACCTAAGA AAGAACGGGA GTCTGTGCTT CCTGAAAAGA CTAAAGAGAA GAAAAGGGCT 
GATCAGTGCA CTGAGTCTTC TAAATCTGGG AAGTCGCGCA CACCACAAAA GAAATCCAAA 
AATGCAGAGG CGACATTTGA GCCCAATTCC ACTGAAGCTC TTGATATGGA CATTGTTTCA 
GTCCCATCTT CTGTGCCAGA GGATATATTT GATAGCTTGG ATGCATCATT AGAGCAGCAA 
AATTCTTTGC AAGATTCTGT AACGAGTACT GCAGTATCTA CACCAAAGTG CAAGGAAGAA 
CTAAAGCCAC TGGTCAAGAA GCAGTTGATT GTGCAGCTGA CACCAGTGTC TCTATCCAAT 
TCCCCAGTGA AAGCAGACAG CAATAGTGCA GGCAAATCGC CAAAGGCTGA CATTGGTAGT 
GATGCTGAAA CGGAGGCGCA GAATCAAAAT AGTGCTTCAT GTGATGAAGT CCTTGAGAAC 
TCATCGGAGA ACGATCTAGA GGAATCTGCT CTCAGGAGAT CACCTCGCGT AAAAACTACT 
CCAAGGAGGC GACAAACTGA TGCAAATTCT GCAGCCTCAA ATACAGAAGA AGAGAGCAAT 
GACACAGACA AAGAGAAAAA AAGCAAAAGG GCAGCATGTT CTAAAGAAAA ACGGAATTCT 
ACAGCAAAGC ATAGCCCTGC AGAAAGTGGG GAGAGCCCCA AAGCAAAAAG ATCATTAGCA 
AAAAAGATGC AGGCACAAGT GAATAAGAGT ACTTCTGATT CAGATGAGGT TCCAGAAGTA 
CTTAAAAAGG CAGCCCTTAT GAGTAACAGC TCCTCAGAGC TTGATAGCAG TAATGAGGCA 
AACAGTGGTA AAAGGACTTC TAATCGTCAA GATAAAACAG CAAAAGGTTA TTCTAAGAGA 
AAAAGAAGTA GCTCAAGTTC TGGTTCTGAG CCAGCCAAAA GGACAACTAG AAACTCAACA 
GCTGCCAAAA AGAAACGTGG GGACCAATCG GACACTTCAA ATTCTGAGCT GGAGAAGGAA 
GTAAAAAAAC TCGGTAAAAA AGATTCTGTC AGGAAGGGCT TAAAGCAGAA ATCTAAGAAA 
AAGGAAGAAA ACAGTGAGTC CTCTGATGAA AAGAATCAAA ATAGATCCCA GAAAGCCAAA 
AAATCCAAAA GAGTGAGAAC ACCTGCCTCT AATAAGAAGG AATCCTCATC CGACCTTTCT 
GAAGAAGAGC AAGAAAATAG CCACAATTCA GATGAAGACA GCGATGAACA AAAGATCAAA 
CCATTAGTGG AAAATGAAGT TGTATCTGCC ACAGGATTTG TTCAATCATC AGGTGATGAA 
GCAGGTACAA AATGTGGGGC AGCTCCTACT GAGGAGGATG ATGACGATGA TGATGACCCT 
GAGAATAGGA TTGCCAAGAA AATGCTTCTG GAAGAAATCA AAGCCAATCT GTCTTCAGAT 
GATGTGGAAG AAGCATCCTC TGATGAAGAG CCAGAAGAAG GCAAAAAATC TGTGGCAAAG 
TCAACTACAA AAACTGAAGA TAAGGATGTT AATGAGGATG ATAAGTCCGA TTCAGATTCA 
GACTCTGACT CTGACTCTGA TTCAGAGTCT GAGAAAAAAC CAAGATATAG ACACCACCTT 
TTGCGCCGCA AGATAAGTTT AAGCGATGGA GATTCTGGAG AAGAAAAAGA GAAAAAAGAC 
AAAGCTAAGA ACTCAAAGAA CGGAAAGCGT CGAAACAGAC GGAAAGTGAG CAGTGAGGAA 
TCGGCCTCTG ACTTCCATAA ATCATCTGGG GTTAGCGAGG AAATTTCAGA GAGTGATGAT 
TCAGATGATC AGCGGCCCAG AACAAGAGCT GCAAAGAAAG AAGCCGAAGA GAACCAGCGT 
AGCTACAAGC AGAAAAAGAA GAGAAGGCGA ATTAAAGTCC AAGATGACTC CAGTGATAGC 
AAGAGTAATA AGGGTAATTC AGAGGATGGA GACCATGATG AGGACTCAGA CTCCAAATCA 
CCTGGTAAGG GCAGGAAGAA GATTAGGAAG ATTATTAAAG ATGACAAGTT GCGAACAGAA 
ACACAGAATG CCTTGAAAGA GGAAGAGGAG AGAAGGAAAA GAATAGCCGA GAGAGAGCGA 
GAACGAGACA AACTAAGAGA GTTACAGGTC GTTGATACTG AAGATGTCTC GCCTGTAAAG 
TGTCCAGTCA CTACTAAACT AGTGTTGGAT GAAAATGAAG AAACAAAAGA GGCTTTGGTG 
CAGGTTCACC AAAAGATGGT TACAAAGTTG AAACCGCATC AGGTAGACGG TGTTCAGTTC 
ATGTGGGACT GTTGCTGTGA GTCTATGAAA AAGACTAAAA AGGACCCTGG TTCTGGCTGT 
ATATTGGCTC ATTGTATGGG TCTTGGCAAG ACTTTACAGG TTGTGACTTT CCTGCACACT 
GTTCTTCTGT CTGAGAAGCT GGATTTCAGC ACTGCTCTTG TGGTGTGTCC TTTAAATACT 
GTTTTAAATT GGCTCAATGA ATTTGAGAAG TGGCAGGATG GCATGGACGA TGAAGAGAAA 
TTGGAGGTCT GTGAACTTGC TACAGTGAAG CGTCCTCAGG AAAGGTCATA CATGATGCAA 
CGCTGGCATC AAGAAGGAGG AATCTTAATA ATTGGATATG AAATGTACCG AAACCTTACT 
CAAGGGAGGA ATGTAAAAAG TAAAAAGTTG AAAGAGATTT TTCACAAGAC CCTTGTGGAT 
CCAGGACCTG ACTTTGTGAT ATGTGATGAA GGACACATCC TCAAAAATGA ATTGTCAGCC 
ATTTCTAAGG CCATGAATTC CATAAAGACA AGAAGAAGAA TCATACTTAC AGGAACCCCA 
CTGCAAAATA ATCTTGTAGA ATATCACTGC ATGGTCAACT TCATCAAGGA AAACCTACTT 
GGTTCCATTA AGGAATTCAG AAATCGTTTC ATCAATCCCA TCCAGAACGG ACAGTGCGCC 
GACTCTACCC TAGTTGATGT CAGAGTGATG AAAAAGAGAG CTCACATCTT GTATGAAATG 
TTAGCTGGCT GTGTTCAGCG AAGAGATTAT ACTGCACTAA CAAAGTTCTT GCCACCAAAG 
CATGAGTATG TATTGTCTGT CAGAATTTCC CCACTTCAGT GTAAGCTTTA CCAATACTAT 
CTGGACCACT TAACAGGAGC TGTTGGTGAA GGAGGTGTAA GAGGAAAATC TGGAACAAAA 
CTTTTCCAGG ATTTCCAGGT CTTGAGCAGA ATCTGGACTC ACCCTTGGTG TTTGCAGCTG 
GACTATATTA GTAAAGAAAA TAAGGGTTAC TTTGATGAAG ACGACAGCAT GGAAGAGTTC 
ATAGCTTCCG ACTCTGAAGA ATCCTCCATG AGTCTGAGTT CTGAGGAAGA AGTTGTTAAT 
AAAAAGAAGG CCAAGGGAAA AGGCAAACGG GAGAACAGCT CAAGTGAAGA AGAAAGTGAC 
AATGATGTTG AAGTTATTAA GGTGTGGAAT TCAAGATCTC GAGGCGGCAA AGGAAAAGAT 
CTGAATGATG AAGAGGCCTC AAACAGCCCT GTAGTTCCTA AAGCTGAAGA AACAAAAGCA 
ACATCCTCTT CCACACCTGG AAGCCCAGCA GCTGACTGGT ACAAGGATTT TGTTACTGAA 
GCAGATTCAT CTATCATGGA GCATTCTGGA AAAATAATGC TTTTGTTTGA GATTTTGCGC 
ATGGCTGAGG TGTTGGGTGA CAAAGTTTTA GTTTTCAGCC AGTCTCTCAT ATCTCTGGAT 
TTGATAGAAG ATTTTCTGGA ATTGGCAAGT ACAGAGAAAA ATGAAGAAAA TGCATCTGAA 
AAGCCAGTAT TTTACAAAGG GGATGGGAAG TGGTTTAGAA ATATCGATTA CTATCGTTTA 
GACGGCCAAA CCTCCCCTCA AACACGTAAA AAGTGGGCAG AGGAGTTCAA TGATTCGACC 
AATATAAGGG GCCGTCTGTT CCTTATTTCT ACAAAAGCTG GATCCCTGGG TATTAATCTT 
GTTGCTGCTA ATCGTGTGAT AATCTTTGAT GCATCATGGA ATCCATCCTA TGATATCCAG 
AGTATCTTCA GGGTGTATCG ATTTGGACAG ATAAAGCCTG TATTTGTCTA CAGGTTTTTA 
GCCCAGGGCA CCATGGAAGA AAAGATATAT GACAGGCAGA TCGCCAAACA GTCCCTGTCC 
TTTAGAGTTA TTGATCAACA ACAAATAGAA CGTCACTTTA CAATGAATGA ACTGACTGAA 
CTTTACACCT TTGAGCCTGA TCTGCTGGAT GACCCTAATT CCGAAAAGAA GAAAAAGCGA 
GAGACTCCCA TGCTGCCTAA GGATACTGTG CTAGCAGAGC TCTTACAAAT CTTGAAGGAG 
TACATAGTCA CTTATCATGA GCACGACTCT CTTCTGGATC ATAAGGAGGA AGAGGCCCTT 
ACAGAAGAGG ATCGGAAGGC AGCATGGGCA GAATATGAAG CTGAAAAGAA GGGTTTGACC 
AATCGTTTTA ACGTGGCAGC AGTAACTGGG GCCAACTACG CTCAAGTCAA TATAAACTCG 
CAATCTTCAT ACATGCCTTT AAACTTGGGA GCGCTGTCTT CTTTATCCAA TCAGCAACTC 
GAGGATTTAA TAAATCAAGG CAGAGAAAAA GTGGTTGAAG CTACAAATAA CATAGCAACT 
GCAAGGATTC AACCCCTTGA AGACATTATC TCGACAGTGT GGAAGGAAAA TCCGACTGCC 
ACGGAGGCCC AAGTTCAGTC CCTTGCCCTA AGCAGGCAGT CGAGCCAAGA ATTGGAAATA 
AAGAGACGTG AGGCCATTTA TAACGACGTA CTGACTAAGC AACAGATGTT GATCAGCTGC 
GTGCAGCGAA TTCTCATGAG TCGAAGGTTA CAGCACCAAT ACAACCAGCA ACAGCAGCAG 
CAACAAATTG TTTTTCAACA GCAAGCAGCT CTGAATCGCC TGATGATGCA GAAAAATCCA 
AATATTATAA TATCCCCTTC TAACTTTTCT CAAATTGACA TGAGAGGAAT GTACCAAGCT 
GGGATGCAAG GTCATCAGAT GCAGCGTAAT GCACCCGCCT CTATAAGAGC AAAAAATCCA 
GGACCTTCCC AGGGAAAAAA AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atrx ( XM_004915742.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915799.1 Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X2, mRNA.

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CloneID	OXa65476
Clone ID Related Accession (Same CDS sequence)	XM_004915742.4
Accession Version	XM_004915742.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional regulator ATRX isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915742.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA ACATGGATGA GGGAAGTACT TCTGAAAAAC TCAAGTCCTT GGGATCATCT 
CGTTCAAAAA GGAAACCCTC ATTTGTCCAA AAATATGTTG GGTCAGATGA AGAGACTGTG 
CCAGATGAAG TTGTCAATTT AGATGGGTCT AATGAGAATT CTGAGAATGA AGTTGATATG 
GAAAGCTTGC CCATAGGTAC AGTGGTTGTA CAACCAGAAC CTGTCTTAAA CGAAGATAAA 
GATGATTTCA AAGGACCAGA GTTTCGAAGC AGAAACAAAC CTAAACCAGA TATTACTATA 
CCTAAGAAAC GCACAGAGGA TGGACTTCAT GGTATTGTTA ACTGCACAGC CTGTGGCCAA 
CAAGTAAATC ATTTTCAGAA GGATTCAATT TATCGACATC CTGCTTTAAA AGTGCTGATA 
TGTAAGGCAT GCTATAAATA TTACATGAGT GATGACATAA ACCGGGATGC AGACGGAATG 
GATGAACAGT GCAGATGGTG TGCTGAAGGA GGAAACTTGA TTTGCTGTGA TTACTGTCAT 
AATGCCTTTT GTAAGAAGTG CATTCTACGA AATCTAGGGA GAAAGGAACT GTCTGCTATT 
ATGGATGAAG AAATGAAGTG GGAGTGCTAC ATTTGCCGAC CAGAACCTTT ACTGGACTCT 
GTAACTGCAT GTGACAGTGT GTTTGAAAAT TTAGAGCAAT TGTTACAACA AAACAAGAAG 
AAAATGAAAT CAGACAATGA AAAAAACAAA TCAAATGAGC ACTCCCAAAA TGATTCTGAT 
AAAAGGAATT CAAGCTACAA TGGACAGGGC AAAAATGTAG GTGATGCACA TGCCGGTACT 
TTAACGTATT CTTATAGAGC CCTTATGGTG CCAAAGGAGA TGCTCAAAAA AGCGAAAAAG 
CTTATTGAGA CAACAACTAG CCTGAATGCA AGTTTTGTAA AGTTTATGAA ACAAGGAACT 
GCGAACAATG AATTGAACCC TGCTGAAAAG TTAAGACAGC TGAAAGCATT TAAATCTGTC 
CTGGGTGACT TAAAGAAAGC CCATGAGGCT TTGGAGGAAA CATTAATGGA AGAGATTCAT 
TCAGTTGACG AGCAAAAGAA ATCTAAGAAA AGCAAAGAGA CTACGAGTGA GAATAAACAT 
GCAAAGGAGA AGAAACTTGG TGAAGAAAAG CAGAAAGAAC CCAAGAAAAT AGTTGCACGT 
GAAAAAGCAA AACCTTCAGA TTCTGACAAT AAGCAAAGGG ATTGTGACAA GAATGCCTCT 
AACGATACAG AGCAGGCTGA AGAAGCGTTG ATACAGAATG GGAAAAATGT AGATCTTACT 
GAAGAAGATA CAATAAAGAG TGAACCCTGT TCAGCTGCTG CTGACGTTAC AGAGGTTTTG 
GCAACAGATG CAGAGGGTAC AGATATAAGT GTCGTCATGG AGGAGGCAGA TGAAAATGTT 
GATGGAAAAA ATGAAAAAGC AACAGTTGAG AGTGATGATA ATGAAGGTAA GCAAAAATCC 
CAGAAAAAGA AGCAGAAGAG TATGCATGAC AAACCTAGGG AAAACTCACC TGGTAACAGA 
AAGAAGAGTG TAGATTCACC TAAGAAAGAA CGGGAGTCTG TGCTTCCTGA AAAGACTAAA 
GAGAAGAAAA GGGCTGATCA GTGCACTGAG TCTTCTAAAT CTGGGAAGTC GCGCACACCA 
CAAAAGAAAT CCAAAAATGC AGAGGCGACA TTTGAGCCCA ATTCCACTGA AGCTCTTGAT 
ATGGACATTG TTTCAGTCCC ATCTTCTGTG CCAGAGGATA TATTTGATAG CTTGGATGCA 
TCATTAGAGC AGCAAAATTC TTTGCAAGAT TCTGTAACGA GTACTGCAGT ATCTACACCA 
AAGTGCAAGG AAGAACTAAA GCCACTGGTC AAGAAGCAGT TGATTGTGCA GCTGACACCA 
GTGTCTCTAT CCAATTCCCC AGTGAAAGCA GACAGCAATA GTGCAGGCAA ATCGCCAAAG 
GCTGACATTG GTAGTGATGC TGAAACGGAG GCGCAGAATC AAAATAGTGC TTCATGTGAT 
GAAGTCCTTG AGAACTCATC GGAGAACGAT CTAGAGGAAT CTGCTCTCAG GAGATCACCT 
CGCGTAAAAA CTACTCCAAG GAGGCGACAA ACTGATGCAA ATTCTGCAGC CTCAAATACA 
GAAGAAGAGA GCAATGACAC AGACAAAGAG AAAAAAAGCA AAAGGGCAGC ATGTTCTAAA 
GAAAAACGGA ATTCTACAGC AAAGCATAGC CCTGCAGAAA GTGGGGAGAG CCCCAAAGCA 
AAAAGATCAT TAGCAAAAAA GATGCAGGCA CAAGTGAATA AGAGTACTTC TGATTCAGAT 
GAGGTTCCAG AAGTACTTAA AAAGGCAGCC CTTATGAGTA ACAGCTCCTC AGAGCTTGAT 
AGCAGTAATG AGGCAAACAG TGGTAAAAGG ACTTCTAATC GTCAAGATAA AACAGCAAAA 
GGTTATTCTA AGAGAAAAAG AAGTAGCTCA AGTTCTGGTT CTGAGCCAGC CAAAAGGACA 
ACTAGAAACT CAACAGCTGC CAAAAAGAAA CGTGGGGACC AATCGGACAC TTCAAATTCT 
GAGCTGGAGA AGGAAGTAAA AAAACTCGGT AAAAAAGATT CTGTCAGGAA GGGCTTAAAG 
CAGAAATCTA AGAAAAAGGA AGAAAACAGT GAGTCCTCTG ATGAAAAGAA TCAAAATAGA 
TCCCAGAAAG CCAAAAAATC CAAAAGAGTG AGAACACCTG CCTCTAATAA GAAGGAATCC 
TCATCCGACC TTTCTGAAGA AGAGCAAGAA AATAGCCACA ATTCAGATGA AGACAGCGAT 
GAACAAAAGA TCAAACCATT AGTGGAAAAT GAAGTTGTAT CTGCCACAGG ATTTGTTCAA 
TCATCAGGTG ATGAAGCAGG TACAAAATGT GGGGCAGCTC CTACTGAGGA GGATGATGAC 
GATGATGATG ACCCTGAGAA TAGGATTGCC AAGAAAATGC TTCTGGAAGA AATCAAAGCC 
AATCTGTCTT CAGATGATGT GGAAGAAGCA TCCTCTGATG AAGAGCCAGA AGAAGGCAAA 
AAATCTGTGG CAAAGTCAAC TACAAAAACT GAAGATAAGG ATGTTAATGA GGATGATAAG 
TCCGATTCAG ATTCAGACTC TGACTCTGAC TCTGATTCAG AGTCTGAGAA AAAACCAAGA 
TATAGACACC ACCTTTTGCG CCGCAAGATA AGTTTAAGCG ATGGAGATTC TGGAGAAGAA 
AAAGAGAAAA AAGACAAAGC TAAGAACTCA AAGAACGGAA AGCGTCGAAA CAGACGGAAA 
GTGAGCAGTG AGGAATCGGC CTCTGACTTC CATAAATCAT CTGGGGTTAG CGAGGAAATT 
TCAGAGAGTG ATGATTCAGA TGATCAGCGG CCCAGAACAA GAGCTGCAAA GAAAGAAGCC 
GAAGAGAACC AGCGTAGCTA CAAGCAGAAA AAGAAGAGAA GGCGAATTAA AGTCCAAGAT 
GACTCCAGTG ATAGCAAGAG TAATAAGGGT AATTCAGAGG ATGGAGACCA TGATGAGGAC 
TCAGACTCCA AATCACCTGG TAAGGGCAGG AAGAAGATTA GGAAGATTAT TAAAGATGAC 
AAGTTGCGAA CAGAAACACA GAATGCCTTG AAAGAGGAAG AGGAGAGAAG GAAAAGAATA 
GCCGAGAGAG AGCGAGAACG AGACAAACTA AGAGAGTTAC AGGTCGTTGA TACTGAAGAT 
GTCTCGCCTG TAAAGTGTCC AGTCACTACT AAACTAGTGT TGGATGAAAA TGAAGAAACA 
AAAGAGGCTT TGGTGCAGGT TCACCAAAAG ATGGTTACAA AGTTGAAACC GCATCAGGTA 
GACGGTGTTC AGTTCATGTG GGACTGTTGC TGTGAGTCTA TGAAAAAGAC TAAAAAGGAC 
CCTGGTTCTG GCTGTATATT GGCTCATTGT ATGGGTCTTG GCAAGACTTT ACAGGTTGTG 
ACTTTCCTGC ACACTGTTCT TCTGTCTGAG AAGCTGGATT TCAGCACTGC TCTTGTGGTG 
TGTCCTTTAA ATACTGTTTT AAATTGGCTC AATGAATTTG AGAAGTGGCA GGATGGCATG 
GACGATGAAG AGAAATTGGA GGTCTGTGAA CTTGCTACAG TGAAGCGTCC TCAGGAAAGG 
TCATACATGA TGCAACGCTG GCATCAAGAA GGAGGAATCT TAATAATTGG ATATGAAATG 
TACCGAAACC TTACTCAAGG GAGGAATGTA AAAAGTAAAA AGTTGAAAGA GATTTTTCAC 
AAGACCCTTG TGGATCCAGG ACCTGACTTT GTGATATGTG ATGAAGGACA CATCCTCAAA 
AATGAATTGT CAGCCATTTC TAAGGCCATG AATTCCATAA AGACAAGAAG AAGAATCATA 
CTTACAGGAA CCCCACTGCA AAATAATCTT GTAGAATATC ACTGCATGGT CAACTTCATC 
AAGGAAAACC TACTTGGTTC CATTAAGGAA TTCAGAAATC GTTTCATCAA TCCCATCCAG 
AACGGACAGT GCGCCGACTC TACCCTAGTT GATGTCAGAG TGATGAAAAA GAGAGCTCAC 
ATCTTGTATG AAATGTTAGC TGGCTGTGTT CAGCGAAGAG ATTATACTGC ACTAACAAAG 
TTCTTGCCAC CAAAGCATGA GTATGTATTG TCTGTCAGAA TTTCCCCACT TCAGTGTAAG 
CTTTACCAAT ACTATCTGGA CCACTTAACA GGAGCTGTTG GTGAAGGAGG TGTAAGAGGA 
AAATCTGGAA CAAAACTTTT CCAGGATTTC CAGGTCTTGA GCAGAATCTG GACTCACCCT 
TGGTGTTTGC AGCTGGACTA TATTAGTAAA GAAAATAAGG GTTACTTTGA TGAAGACGAC 
AGCATGGAAG AGTTCATAGC TTCCGACTCT GAAGAATCCT CCATGAGTCT GAGTTCTGAG 
GAAGAAGTTG TTAATAAAAA GAAGGCCAAG GGAAAAGGCA AACGGGAGAA CAGCTCAAGT 
GAAGAAGAAA GTGACAATGA TGTTGAAGTT ATTAAGGTGT GGAATTCAAG ATCTCGAGGC 
GGCAAAGGAA AAGATCTGAA TGATGAAGAG GCCTCAAACA GCCCTGTAGT TCCTAAAGCT 
GAAGAAACAA AAGCAACATC CTCTTCCACA CCTGGAAGCC CAGCAGCTGA CTGGTACAAG 
GATTTTGTTA CTGAAGCAGA TTCATCTATC ATGGAGCATT CTGGAAAAAT AATGCTTTTG 
TTTGAGATTT TGCGCATGGC TGAGGTGTTG GGTGACAAAG TTTTAGTTTT CAGCCAGTCT 
CTCATATCTC TGGATTTGAT AGAAGATTTT CTGGAATTGG CAAGTACAGA GAAAAATGAA 
GAAAATGCAT CTGAAAAGCC AGTATTTTAC AAAGGGGATG GGAAGTGGTT TAGAAATATC 
GATTACTATC GTTTAGACGG CCAAACCTCC CCTCAAACAC GTAAAAAGTG GGCAGAGGAG 
TTCAATGATT CGACCAATAT AAGGGGCCGT CTGTTCCTTA TTTCTACAAA AGCTGGATCC 
CTGGGTATTA ATCTTGTTGC TGCTAATCGT GTGATAATCT TTGATGCATC ATGGAATCCA 
TCCTATGATA TCCAGAGTAT CTTCAGGGTG TATCGATTTG GACAGATAAA GCCTGTATTT 
GTCTACAGGT TTTTAGCCCA GGGCACCATG GAAGAAAAGA TATATGACAG GCAGATCGCC 
AAACAGTCCC TGTCCTTTAG AGTTATTGAT CAACAACAAA TAGAACGTCA CTTTACAATG 
AATGAACTGA CTGAACTTTA CACCTTTGAG CCTGATCTGC TGGATGACCC TAATTCCGAA 
AAGAAGAAAA AGCGAGAGAC TCCCATGCTG CCTAAGGATA CTGTGCTAGC AGAGCTCTTA 
CAAATCTTGA AGGAGTACAT AGTCACTTAT CATGAGCACG ACTCTCTTCT GGATCATAAG 
GAGGAAGAGG CCCTTACAGA AGAGGATCGG AAGGCAGCAT GGGCAGAATA TGAAGCTGAA 
AAGAAGGGTT TGACCAATCG TTTTAACGTG GCAGCAGTAA CTGGGGCCAA CTACGCTCAA 
GTCAATATAA ACTCGCAATC TTCATACATG CCTTTAAACT TGGGAGCGCT GTCTTCTTTA 
TCCAATCAGC AACTCGAGGA TTTAATAAAT CAAGGCAGAG AAAAAGTGGT TGAAGCTACA 
AATAACATAG CAACTGCAAG GATTCAACCC CTTGAAGACA TTATCTCGAC AGTGTGGAAG 
GAAAATCCGA CTGCCACGGA GGCCCAAGTT CAGTCCCTTG CCCTAAGCAG GCAGTCGAGC 
CAAGAATTGG AAATAAAGAG ACGTGAGGCC ATTTATAACG ACGTACTGAC TAAGCAACAG 
ATGTTGATCA GCTGCGTGCA GCGAATTCTC ATGAGTCGAA GGTTACAGCA CCAATACAAC 
CAGCAACAGC AGCAGCAACA AATTGTTTTT CAACAGCAAG CAGCTCTGAA TCGCCTGATG 
ATGCAGAAAA ATCCAAATAT TATAATATCC CCTTCTAACT TTTCTCAAAT TGACATGAGA 
GGAATGTACC AAGCTGGGAT GCAAGGTCAT CAGATGCAGC GTAATGCACC CGCCTCTATA 
AGAGCAAAAA ATCCAGGACC TTCCCAGGGA AAAAAAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915799.1
CDS	390..7031
Translation	MENNMDEGSTSEKLKSLGSSRSKRKPSFVQKYVGSDEETVPDEVVNLDGSNENSENEVDMESLPIGTVVVQPEPVLNEDKDDFKGPEFRSRNKPKPDITIPKKRTEDGLHGIVNCTACGQQVNHFQKDSIYRHPALKVLICKACYKYYMSDDINRDADGMDEQCRWCAEGGNLICCDYCHNAFCKKCILRNLGRKELSAIMDEEMKWECYICRPEPLLDSVTACDSVFENLEQLLQQNKKKMKSDNEKNKSNEHSQNDSDKRNSSYNGQGKNVGDAHAGTLTYSYRALMVPKEMLKKAKKLIETTTSLNASFVKFMKQGTANNELNPAEKLRQLKAFKSVLGDLKKAHEALEETLMEEIHSVDEQKKSKKSKETTSENKHAKEKKLGEEKQKEPKKIVAREKAKPSDSDNKQRDCDKNASNDTEQAEEALIQNGKNVDLTEEDTIKSEPCSAAADVTEVLATDAEGTDISVVMEEADENVDGKNEKATVESDDNEGKQKSQKKKQKSMHDKPRENSPGNRKKSVDSPKKERESVLPEKTKEKKRADQCTESSKSGKSRTPQKKSKNAEATFEPNSTEALDMDIVSVPSSVPEDIFDSLDASLEQQNSLQDSVTSTAVSTPKCKEELKPLVKKQLIVQLTPVSLSNSPVKADSNSAGKSPKADIGSDAETEAQNQNSASCDEVLENSSENDLEESALRRSPRVKTTPRRRQTDANSAASNTEEESNDTDKEKKSKRAACSKEKRNSTAKHSPAESGESPKAKRSLAKKMQAQVNKSTSDSDEVPEVLKKAALMSNSSSELDSSNEANSGKRTSNRQDKTAKGYSKRKRSSSSSGSEPAKRTTRNSTAAKKKRGDQSDTSNSELEKEVKKLGKKDSVRKGLKQKSKKKEENSESSDEKNQNRSQKAKKSKRVRTPASNKKESSSDLSEEEQENSHNSDEDSDEQKIKPLVENEVVSATGFVQSSGDEAGTKCGAAPTEEDDDDDDDPENRIAKKMLLEEIKANLSSDDVEEASSDEEPEEGKKSVAKSTTKTEDKDVNEDDKSDSDSDSDSDSDSESEKKPRYRHHLLRRKISLSDGDSGEEKEKKDKAKNSKNGKRRNRRKVSSEESASDFHKSSGVSEEISESDDSDDQRPRTRAAKKEAEENQRSYKQKKKRRRIKVQDDSSDSKSNKGNSEDGDHDEDSDSKSPGKGRKKIRKIIKDDKLRTETQNALKEEEERRKRIAERERERDKLRELQVVDTEDVSPVKCPVTTKLVLDENEETKEALVQVHQKMVTKLKPHQVDGVQFMWDCCCESMKKTKKDPGSGCILAHCMGLGKTLQVVTFLHTVLLSEKLDFSTALVVCPLNTVLNWLNEFEKWQDGMDDEEKLEVCELATVKRPQERSYMMQRWHQEGGILIIGYEMYRNLTQGRNVKSKKLKEIFHKTLVDPGPDFVICDEGHILKNELSAISKAMNSIKTRRRIILTGTPLQNNLVEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQCADSTLVDVRVMKKRAHILYEMLAGCVQRRDYTALTKFLPPKHEYVLSVRISPLQCKLYQYYLDHLTGAVGEGGVRGKSGTKLFQDFQVLSRIWTHPWCLQLDYISKENKGYFDEDDSMEEFIASDSEESSMSLSSEEEVVNKKKAKGKGKRENSSSEEESDNDVEVIKVWNSRSRGGKGKDLNDEEASNSPVVPKAEETKATSSSTPGSPAADWYKDFVTEADSSIMEHSGKIMLLFEILRMAEVLGDKVLVFSQSLISLDLIEDFLELASTEKNEENASEKPVFYKGDGKWFRNIDYYRLDGQTSPQTRKKWAEEFNDSTNIRGRLFLISTKAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQIKPVFVYRFLAQGTMEEKIYDRQIAKQSLSFRVIDQQQIERHFTMNELTELYTFEPDLLDDPNSEKKKKRETPMLPKDTVLAELLQILKEYIVTYHEHDSLLDHKEEEALTEEDRKAAWAEYEAEKKGLTNRFNVAAVTGANYAQVNINSQSSYMPLNLGALSSLSNQQLEDLINQGREKVVEATNNIATARIQPLEDIISTVWKENPTATEAQVQSLALSRQSSQELEIKRREAIYNDVLTKQQMLISCVQRILMSRRLQHQYNQQQQQQQIVFQQQAALNRLMMQKNPNIIISPSNFSQIDMRGMYQAGMQGHQMQRNAPASIRAKNPGPSQGKKM

Target ORF information:

RefSeq Version	XM_004915742.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915742.4

ORF Insert Sequence:

ATGGAAAACA ACATGGATGA GGGAAGTACT TCTGAAAAAC TCAAGTCCTT GGGATCATCT 
CGTTCAAAAA GGAAACCCTC ATTTGTCCAA AAATATGTTG GGTCAGATGA AGAGACTGTG 
CCAGATGAAG TTGTCAATTT AGATGGGTCT AATGAGAATT CTGAGAATGA AGTTGATATG 
GAAAGCTTGC CCATAGGTAC AGTGGTTGTA CAACCAGAAC CTGTCTTAAA CGAAGATAAA 
GATGATTTCA AAGGACCAGA GTTTCGAAGC AGAAACAAAC CTAAACCAGA TATTACTATA 
CCTAAGAAAC GCACAGAGGA TGGACTTCAT GGTATTGTTA ACTGCACAGC CTGTGGCCAA 
CAAGTAAATC ATTTTCAGAA GGATTCAATT TATCGACATC CTGCTTTAAA AGTGCTGATA 
TGTAAGGCAT GCTATAAATA TTACATGAGT GATGACATAA ACCGGGATGC AGACGGAATG 
GATGAACAGT GCAGATGGTG TGCTGAAGGA GGAAACTTGA TTTGCTGTGA TTACTGTCAT 
AATGCCTTTT GTAAGAAGTG CATTCTACGA AATCTAGGGA GAAAGGAACT GTCTGCTATT 
ATGGATGAAG AAATGAAGTG GGAGTGCTAC ATTTGCCGAC CAGAACCTTT ACTGGACTCT 
GTAACTGCAT GTGACAGTGT GTTTGAAAAT TTAGAGCAAT TGTTACAACA AAACAAGAAG 
AAAATGAAAT CAGACAATGA AAAAAACAAA TCAAATGAGC ACTCCCAAAA TGATTCTGAT 
AAAAGGAATT CAAGCTACAA TGGACAGGGC AAAAATGTAG GTGATGCACA TGCCGGTACT 
TTAACGTATT CTTATAGAGC CCTTATGGTG CCAAAGGAGA TGCTCAAAAA AGCGAAAAAG 
CTTATTGAGA CAACAACTAG CCTGAATGCA AGTTTTGTAA AGTTTATGAA ACAAGGAACT 
GCGAACAATG AATTGAACCC TGCTGAAAAG TTAAGACAGC TGAAAGCATT TAAATCTGTC 
CTGGGTGACT TAAAGAAAGC CCATGAGGCT TTGGAGGAAA CATTAATGGA AGAGATTCAT 
TCAGTTGACG AGCAAAAGAA ATCTAAGAAA AGCAAAGAGA CTACGAGTGA GAATAAACAT 
GCAAAGGAGA AGAAACTTGG TGAAGAAAAG CAGAAAGAAC CCAAGAAAAT AGTTGCACGT 
GAAAAAGCAA AACCTTCAGA TTCTGACAAT AAGCAAAGGG ATTGTGACAA GAATGCCTCT 
AACGATACAG AGCAGGCTGA AGAAGCGTTG ATACAGAATG GGAAAAATGT AGATCTTACT 
GAAGAAGATA CAATAAAGAG TGAACCCTGT TCAGCTGCTG CTGACGTTAC AGAGGTTTTG 
GCAACAGATG CAGAGGGTAC AGATATAAGT GTCGTCATGG AGGAGGCAGA TGAAAATGTT 
GATGGAAAAA ATGAAAAAGC AACAGTTGAG AGTGATGATA ATGAAGGTAA GCAAAAATCC 
CAGAAAAAGA AGCAGAAGAG TATGCATGAC AAACCTAGGG AAAACTCACC TGGTAACAGA 
AAGAAGAGTG TAGATTCACC TAAGAAAGAA CGGGAGTCTG TGCTTCCTGA AAAGACTAAA 
GAGAAGAAAA GGGCTGATCA GTGCACTGAG TCTTCTAAAT CTGGGAAGTC GCGCACACCA 
CAAAAGAAAT CCAAAAATGC AGAGGCGACA TTTGAGCCCA ATTCCACTGA AGCTCTTGAT 
ATGGACATTG TTTCAGTCCC ATCTTCTGTG CCAGAGGATA TATTTGATAG CTTGGATGCA 
TCATTAGAGC AGCAAAATTC TTTGCAAGAT TCTGTAACGA GTACTGCAGT ATCTACACCA 
AAGTGCAAGG AAGAACTAAA GCCACTGGTC AAGAAGCAGT TGATTGTGCA GCTGACACCA 
GTGTCTCTAT CCAATTCCCC AGTGAAAGCA GACAGCAATA GTGCAGGCAA ATCGCCAAAG 
GCTGACATTG GTAGTGATGC TGAAACGGAG GCGCAGAATC AAAATAGTGC TTCATGTGAT 
GAAGTCCTTG AGAACTCATC GGAGAACGAT CTAGAGGAAT CTGCTCTCAG GAGATCACCT 
CGCGTAAAAA CTACTCCAAG GAGGCGACAA ACTGATGCAA ATTCTGCAGC CTCAAATACA 
GAAGAAGAGA GCAATGACAC AGACAAAGAG AAAAAAAGCA AAAGGGCAGC ATGTTCTAAA 
GAAAAACGGA ATTCTACAGC AAAGCATAGC CCTGCAGAAA GTGGGGAGAG CCCCAAAGCA 
AAAAGATCAT TAGCAAAAAA GATGCAGGCA CAAGTGAATA AGAGTACTTC TGATTCAGAT 
GAGGTTCCAG AAGTACTTAA AAAGGCAGCC CTTATGAGTA ACAGCTCCTC AGAGCTTGAT 
AGCAGTAATG AGGCAAACAG TGGTAAAAGG ACTTCTAATC GTCAAGATAA AACAGCAAAA 
GGTTATTCTA AGAGAAAAAG AAGTAGCTCA AGTTCTGGTT CTGAGCCAGC CAAAAGGACA 
ACTAGAAACT CAACAGCTGC CAAAAAGAAA CGTGGGGACC AATCGGACAC TTCAAATTCT 
GAGCTGGAGA AGGAAGTAAA AAAACTCGGT AAAAAAGATT CTGTCAGGAA GGGCTTAAAG 
CAGAAATCTA AGAAAAAGGA AGAAAACAGT GAGTCCTCTG ATGAAAAGAA TCAAAATAGA 
TCCCAGAAAG CCAAAAAATC CAAAAGAGTG AGAACACCTG CCTCTAATAA GAAGGAATCC 
TCATCCGACC TTTCTGAAGA AGAGCAAGAA AATAGCCACA ATTCAGATGA AGACAGCGAT 
GAACAAAAGA TCAAACCATT AGTGGAAAAT GAAGTTGTAT CTGCCACAGG ATTTGTTCAA 
TCATCAGGTG ATGAAGCAGG TACAAAATGT GGGGCAGCTC CTACTGAGGA GGATGATGAC 
GATGATGATG ACCCTGAGAA TAGGATTGCC AAGAAAATGC TTCTGGAAGA AATCAAAGCC 
AATCTGTCTT CAGATGATGT GGAAGAAGCA TCCTCTGATG AAGAGCCAGA AGAAGGCAAA 
AAATCTGTGG CAAAGTCAAC TACAAAAACT GAAGATAAGG ATGTTAATGA GGATGATAAG 
TCCGATTCAG ATTCAGACTC TGACTCTGAC TCTGATTCAG AGTCTGAGAA AAAACCAAGA 
TATAGACACC ACCTTTTGCG CCGCAAGATA AGTTTAAGCG ATGGAGATTC TGGAGAAGAA 
AAAGAGAAAA AAGACAAAGC TAAGAACTCA AAGAACGGAA AGCGTCGAAA CAGACGGAAA 
GTGAGCAGTG AGGAATCGGC CTCTGACTTC CATAAATCAT CTGGGGTTAG CGAGGAAATT 
TCAGAGAGTG ATGATTCAGA TGATCAGCGG CCCAGAACAA GAGCTGCAAA GAAAGAAGCC 
GAAGAGAACC AGCGTAGCTA CAAGCAGAAA AAGAAGAGAA GGCGAATTAA AGTCCAAGAT 
GACTCCAGTG ATAGCAAGAG TAATAAGGGT AATTCAGAGG ATGGAGACCA TGATGAGGAC 
TCAGACTCCA AATCACCTGG TAAGGGCAGG AAGAAGATTA GGAAGATTAT TAAAGATGAC 
AAGTTGCGAA CAGAAACACA GAATGCCTTG AAAGAGGAAG AGGAGAGAAG GAAAAGAATA 
GCCGAGAGAG AGCGAGAACG AGACAAACTA AGAGAGTTAC AGGTCGTTGA TACTGAAGAT 
GTCTCGCCTG TAAAGTGTCC AGTCACTACT AAACTAGTGT TGGATGAAAA TGAAGAAACA 
AAAGAGGCTT TGGTGCAGGT TCACCAAAAG ATGGTTACAA AGTTGAAACC GCATCAGGTA 
GACGGTGTTC AGTTCATGTG GGACTGTTGC TGTGAGTCTA TGAAAAAGAC TAAAAAGGAC 
CCTGGTTCTG GCTGTATATT GGCTCATTGT ATGGGTCTTG GCAAGACTTT ACAGGTTGTG 
ACTTTCCTGC ACACTGTTCT TCTGTCTGAG AAGCTGGATT TCAGCACTGC TCTTGTGGTG 
TGTCCTTTAA ATACTGTTTT AAATTGGCTC AATGAATTTG AGAAGTGGCA GGATGGCATG 
GACGATGAAG AGAAATTGGA GGTCTGTGAA CTTGCTACAG TGAAGCGTCC TCAGGAAAGG 
TCATACATGA TGCAACGCTG GCATCAAGAA GGAGGAATCT TAATAATTGG ATATGAAATG 
TACCGAAACC TTACTCAAGG GAGGAATGTA AAAAGTAAAA AGTTGAAAGA GATTTTTCAC 
AAGACCCTTG TGGATCCAGG ACCTGACTTT GTGATATGTG ATGAAGGACA CATCCTCAAA 
AATGAATTGT CAGCCATTTC TAAGGCCATG AATTCCATAA AGACAAGAAG AAGAATCATA 
CTTACAGGAA CCCCACTGCA AAATAATCTT GTAGAATATC ACTGCATGGT CAACTTCATC 
AAGGAAAACC TACTTGGTTC CATTAAGGAA TTCAGAAATC GTTTCATCAA TCCCATCCAG 
AACGGACAGT GCGCCGACTC TACCCTAGTT GATGTCAGAG TGATGAAAAA GAGAGCTCAC 
ATCTTGTATG AAATGTTAGC TGGCTGTGTT CAGCGAAGAG ATTATACTGC ACTAACAAAG 
TTCTTGCCAC CAAAGCATGA GTATGTATTG TCTGTCAGAA TTTCCCCACT TCAGTGTAAG 
CTTTACCAAT ACTATCTGGA CCACTTAACA GGAGCTGTTG GTGAAGGAGG TGTAAGAGGA 
AAATCTGGAA CAAAACTTTT CCAGGATTTC CAGGTCTTGA GCAGAATCTG GACTCACCCT 
TGGTGTTTGC AGCTGGACTA TATTAGTAAA GAAAATAAGG GTTACTTTGA TGAAGACGAC 
AGCATGGAAG AGTTCATAGC TTCCGACTCT GAAGAATCCT CCATGAGTCT GAGTTCTGAG 
GAAGAAGTTG TTAATAAAAA GAAGGCCAAG GGAAAAGGCA AACGGGAGAA CAGCTCAAGT 
GAAGAAGAAA GTGACAATGA TGTTGAAGTT ATTAAGGTGT GGAATTCAAG ATCTCGAGGC 
GGCAAAGGAA AAGATCTGAA TGATGAAGAG GCCTCAAACA GCCCTGTAGT TCCTAAAGCT 
GAAGAAACAA AAGCAACATC CTCTTCCACA CCTGGAAGCC CAGCAGCTGA CTGGTACAAG 
GATTTTGTTA CTGAAGCAGA TTCATCTATC ATGGAGCATT CTGGAAAAAT AATGCTTTTG 
TTTGAGATTT TGCGCATGGC TGAGGTGTTG GGTGACAAAG TTTTAGTTTT CAGCCAGTCT 
CTCATATCTC TGGATTTGAT AGAAGATTTT CTGGAATTGG CAAGTACAGA GAAAAATGAA 
GAAAATGCAT CTGAAAAGCC AGTATTTTAC AAAGGGGATG GGAAGTGGTT TAGAAATATC 
GATTACTATC GTTTAGACGG CCAAACCTCC CCTCAAACAC GTAAAAAGTG GGCAGAGGAG 
TTCAATGATT CGACCAATAT AAGGGGCCGT CTGTTCCTTA TTTCTACAAA AGCTGGATCC 
CTGGGTATTA ATCTTGTTGC TGCTAATCGT GTGATAATCT TTGATGCATC ATGGAATCCA 
TCCTATGATA TCCAGAGTAT CTTCAGGGTG TATCGATTTG GACAGATAAA GCCTGTATTT 
GTCTACAGGT TTTTAGCCCA GGGCACCATG GAAGAAAAGA TATATGACAG GCAGATCGCC 
AAACAGTCCC TGTCCTTTAG AGTTATTGAT CAACAACAAA TAGAACGTCA CTTTACAATG 
AATGAACTGA CTGAACTTTA CACCTTTGAG CCTGATCTGC TGGATGACCC TAATTCCGAA 
AAGAAGAAAA AGCGAGAGAC TCCCATGCTG CCTAAGGATA CTGTGCTAGC AGAGCTCTTA 
CAAATCTTGA AGGAGTACAT AGTCACTTAT CATGAGCACG ACTCTCTTCT GGATCATAAG 
GAGGAAGAGG CCCTTACAGA AGAGGATCGG AAGGCAGCAT GGGCAGAATA TGAAGCTGAA 
AAGAAGGGTT TGACCAATCG TTTTAACGTG GCAGCAGTAA CTGGGGCCAA CTACGCTCAA 
GTCAATATAA ACTCGCAATC TTCATACATG CCTTTAAACT TGGGAGCGCT GTCTTCTTTA 
TCCAATCAGC AACTCGAGGA TTTAATAAAT CAAGGCAGAG AAAAAGTGGT TGAAGCTACA 
AATAACATAG CAACTGCAAG GATTCAACCC CTTGAAGACA TTATCTCGAC AGTGTGGAAG 
GAAAATCCGA CTGCCACGGA GGCCCAAGTT CAGTCCCTTG CCCTAAGCAG GCAGTCGAGC 
CAAGAATTGG AAATAAAGAG ACGTGAGGCC ATTTATAACG ACGTACTGAC TAAGCAACAG 
ATGTTGATCA GCTGCGTGCA GCGAATTCTC ATGAGTCGAA GGTTACAGCA CCAATACAAC 
CAGCAACAGC AGCAGCAACA AATTGTTTTT CAACAGCAAG CAGCTCTGAA TCGCCTGATG 
ATGCAGAAAA ATCCAAATAT TATAATATCC CCTTCTAACT TTTCTCAAAT TGACATGAGA 
GGAATGTACC AAGCTGGGAT GCAAGGTCAT CAGATGCAGC GTAATGCACC CGCCTCTATA 
AGAGCAAAAA ATCCAGGACC TTCCCAGGGA AAAAAAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atrx ( XM_002931812.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931858.1 Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X1, mRNA.

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CloneID	OXa12505
Clone ID Related Accession (Same CDS sequence)	XM_002931812.4
Accession Version	XM_002931812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional regulator ATRX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCGG AGCCCATGAG TGAAAGCAAG TTAAATACAT TGGTGAAGAA ACTTCATGAT 
TTTCTAGCTC ACTCCTCAGA AGAATCTGAG GACACAACTT CTCCAACAAG ACAGTCTGTG 
AGCAGAAGTG CAGCTAAAGT CAAGTCTGTA CACCGACTGC ATCCAATGGA AAACAACATG 
GATGAGGGAA GTACTTCTGA AAAACTCAAG TCCTTGGGAT CATCTCGTTC AAAAAGGAAA 
CCCTCATTTG TCCAAAAATA TGTTGGGTCA GATGAAGAGA CTGTGCCAGA TGAAGTTGTC 
AATTTAGATG GGTCTAATGA GAATTCTGAG AATGAAGTTG ATATGGAAAG CTTGCCCATA 
GGTACAGTGG TTGTACAACC AGAACCTGTC TTAAACGAAG ATAAAGATGA TTTCAAAGGA 
CCAGAGTTTC GAAGCAGAAA CAAACCTAAA CCAGATATTA CTATACCTAA GAAACGCACA 
GAGGATGGAC TTCATGGTAT TGTTAACTGC ACAGCCTGTG GCCAACAAGT AAATCATTTT 
CAGAAGGATT CAATTTATCG ACATCCTGCT TTAAAAGTGC TGATATGTAA GGCATGCTAT 
AAATATTACA TGAGTGATGA CATAAACCGG GATGCAGACG GAATGGATGA ACAGTGCAGA 
TGGTGTGCTG AAGGAGGAAA CTTGATTTGC TGTGATTACT GTCATAATGC CTTTTGTAAG 
AAGTGCATTC TACGAAATCT AGGGAGAAAG GAACTGTCTG CTATTATGGA TGAAGAAATG 
AAGTGGGAGT GCTACATTTG CCGACCAGAA CCTTTACTGG ACTCTGTAAC TGCATGTGAC 
AGTGTGTTTG AAAATTTAGA GCAATTGTTA CAACAAAACA AGAAGAAAAT GAAATCAGAC 
AATGAAAAAA ACAAATCAAA TGAGCACTCC CAAAATGATT CTGATAAAAG GAATTCAAGC 
TACAATGGAC AGGGCAAAAA TGTAGGTGAT GCACATGCCG GTACTTTAAC GTATTCTTAT 
AGAGCCCTTA TGGTGCCAAA GGAGATGCTC AAAAAAGCGA AAAAGCTTAT TGAGACAACA 
ACTAGCCTGA ATGCAAGTTT TGTAAAGTTT ATGAAACAAG GAACTGCGAA CAATGAATTG 
AACCCTGCTG AAAAGTTAAG ACAGCTGAAA GCATTTAAAT CTGTCCTGGG TGACTTAAAG 
AAAGCCCATG AGGCTTTGGA GGAAACATTA ATGGAAGAGA TTCATTCAGT TGACGAGCAA 
AAAAAATCTA AGAAAAGCAA AGAGACTACG AGTGAGAATA AACATGCAAA GGAGAAGAAA 
CTTGGTGAAG AAAAGCAGAA AGAACCCAAG AAAATAGTTG CACGTGAAAA AGCAAAACCT 
TCAGATTCTG ACAATAAGCA AAGGGATTGT GACAAGAATG CCTCTAACGA TACAGAGCAG 
GCTGAAGAAG CGTTGATACA GAATGGGAAA AATGTAGATC TTACTGAAGA AGATACAATA 
AAGAGTGAAC CCTGTTCAGC TGCTGCTGAC GTTACAGAGG TTTTGGCAAC AGATGCAGAG 
GGTACAGATA TAAGTGTCGT CATGGAGGAG GCAGATGAAA ATGTTGATGG AAAAAATGAA 
AAAGCAACAG TTGAGAGTGA TGATAATGAA GGTAAGCAAA AATCCCAGAA AAAGAAGCAG 
AAGAGTATGC ATGACAAACC TAGGGAAAAC TCACCTGGTA ACAGAAAGAA GAGTGTAGAT 
TCACCTAAGA AAGAACGGGA GTCTGTGCTT CCTGAAAAGA CTAAAGAGAA GAAAAGGGCT 
GATCAGTGCA CTGAGTCTTC TAAATCTGGG AAGTCGCGCA CACCACAAAA GAAATCCAAA 
AATGCAGAGG CGACATTTGA GCCCAATTCC ACTGAAGCTC TTGATATGGA CATTGTTTCA 
GTCCCATCTT CTGTGCCAGA GGATATATTT GATAGCTTGG ATGCATCATT AGAGCAGCAA 
AATTCTTTGC AAGATTCTGT AACGAGTACT GCAGTATCTA CACCAAAGTG CAAGGAAGAA 
CTAAAGCCAC TGGTCAAGAA GCAGTTGATT GTGCAGCTGA CACCAGTGTC TCTATCCAAT 
TCCCCAGTGA AAGCAGACAG CAATAGTGCA GGCAAATCGC CAAAGGCTGA CATTGGTAGT 
GATGCTGAAA CGGAGGCGCA GAATCAAAAT AGTGCTTCAT GTGATGAAGT CCTTGAGAAC 
TCATCGGAGA ACGATCTAGA GGAATCTGCT CTCAGGAGAT CACCTCGCGT AAAAACTACT 
CCAAGGAGGC GACAAACTGA TGCAAATTCT GCAGCCTCAA ATACAGAAGA AGAGAGCAAT 
GACACAGACA AAGAGAAAAA AAGCAAAAGG GCAGCATGTT CTAAAGAAAA ACGGAATTCT 
ACAGCAAAGC ATAGCCCTGC AGAAAGTGGG GAGAGCCCCA AAGCAAAAAG ATCATTAGCA 
AAAAAGATGC AGGCACAAGT GAATAAGAGT ACTTCTGATT CAGATGAGGT TCCAGAAGTA 
CTTAAAAAGG CAGCCCTTAT GAGTAACAGC TCCTCAGAGC TTGATAGCAG TAATGAGGCA 
AACAGTGGTA AAAGGACTTC TAATCGTCAA GATAAAACAG CAAAAGGTTA TTCTAAGAGA 
AAAAGAAGTA GCTCAAGTTC TGGTTCTGAG CCAGCCAAAA GGACAACTAG AAACTCAACA 
GCTGCCAAAA AGAAACGTGG GGACCAATCG GACACTTCAA ATTCTGAGCT GGAGAAGGAA 
GTAAAAAAAC TCGGTAAAAA AGATTCTGTC AGGAAGGGCT TAAAGCAGAA ATCTAAGAAA 
AAGGAAGAAA ACAGTGAGTC CTCTGATGAA AAGAATCAAA ATAGATCCCA GAAAGCCAAA 
AAATCCAAAA GAGTGAGAAC ACCTGCCTCT AATAAGAAGG AATCCTCATC CGACCTTTCT 
GAAGAAGAGC AAGAAAATAG CCACAATTCA GATGAAGACA GCGATGAACA AAAGATCAAA 
CCATTAGTGG AAAATGAAGT TGTATCTGCC ACAGGATTTG TTCAATCATC AGGTGATGAA 
GCAGGTACAA AATGTGGGGC AGCTCCTACT GAGGAGGATG ATGACGATGA TGATGACCCT 
GAGAATAGGA TTGCCAAGAA AATGCTTCTG GAAGAAATCA AAGCCAATCT GTCTTCAGAT 
GATGTGGAAG AAGCATCCTC TGATGAAGAG CCAGAAGAAG GCAAAAAATC TGTGGCAAAG 
TCAACTACAA AAACTGAAGA TAAGGATGTT AATGAGGATG ATAAGTCCGA TTCAGATTCA 
GACTCTGACT CTGACTCTGA TTCAGAGTCT GAGAAAAAAC CAAGATATAG ACACCACCTT 
TTGCGCCGCA AGATAAGTTT AAGCGATGGA GATTCTGGAG AAGAAAAAGA GAAAAAAGAC 
AAAGCTAAGA ACTCAAAGAA CGGAAAGCGT CGAAACAGAC GGAAAGTGAG CAGTGAGGAA 
TCGGCCTCTG ACTTCCATAA ATCATCTGGG GTTAGCGAGG AAATTTCAGA GAGTGATGAT 
TCAGATGATC AGCGGCCCAG AACAAGAGCT GCAAAGAAAG AAGCCGAAGA GAACCAGCGT 
AGCTACAAGC AGAAAAAGAA GAGAAGGCGA ATTAAAGTCC AAGATGACTC CAGTGATAGC 
AAGAGTAATA AGGGTAATTC AGAGGATGGA GACCATGATG AGGACTCAGA CTCCAAATCA 
CCTGGTAAGG GCAGGAAGAA GATTAGGAAG ATTATTAAAG ATGACAAGTT GCGAACAGAA 
ACACAGAATG CCTTGAAAGA GGAAGAGGAG AGAAGGAAAA GAATAGCCGA GAGAGAGCGA 
GAACGAGACA AACTAAGAGA GTTACAGGTC GTTGATACTG AAGATGTCTC GCCTGTAAAG 
TGTCCAGTCA CTACTAAACT AGTGTTGGAT GAAAATGAAG AAACAAAAGA GGCTTTGGTG 
CAGGTTCACC AAAAGATGGT TACAAAGTTG AAACCGCATC AGGTAGACGG TGTTCAGTTC 
ATGTGGGACT GTTGCTGTGA GTCTATGAAA AAGACTAAAA AGGACCCTGG TTCTGGCTGT 
ATATTGGCTC ATTGTATGGG TCTTGGCAAG ACTTTACAGG TTGTGACTTT CCTGCACACT 
GTTCTTCTGT CTGAGAAGCT GGATTTCAGC ACTGCTCTTG TGGTGTGTCC TTTAAATACT 
GTTTTAAATT GGCTCAATGA ATTTGAGAAG TGGCAGGATG GCATGGACGA TGAAGAGAAA 
TTGGAGGTCT GTGAACTTGC TACAGTGAAG CGTCCTCAGG AAAGGTCATA CATGATGCAA 
CGCTGGCATC AAGAAGGAGG AATCTTAATA ATTGGATATG AAATGTACCG AAACCTTACT 
CAAGGGAGGA ATGTAAAAAG TAAAAAGTTG AAAGAGATTT TTCACAAGAC CCTTGTGGAT 
CCAGGACCTG ACTTTGTGAT ATGTGATGAA GGACACATCC TCAAAAATGA ATTGTCAGCC 
ATTTCTAAGG CCATGAATTC CATAAAGACA AGAAGAAGAA TCATACTTAC AGGAACCCCA 
CTGCAAAATA ATCTTGTAGA ATATCACTGC ATGGTCAACT TCATCAAGGA AAACCTACTT 
GGTTCCATTA AGGAATTCAG AAATCGTTTC ATCAATCCCA TCCAGAACGG ACAGTGCGCC 
GACTCTACCC TAGTTGATGT CAGAGTGATG AAAAAGAGAG CTCACATCTT GTATGAAATG 
TTAGCTGGCT GTGTTCAGCG AAGAGATTAT ACTGCACTAA CAAAGTTCTT GCCACCAAAG 
CATGAGTATG TATTGTCTGT CAGAATTTCC CCACTTCAGT GTAAGCTTTA CCAATACTAT 
CTGGACCACT TAACAGGAGC TGTTGGTGAA GGAGGTGTAA GAGGAAAATC TGGAACAAAA 
CTTTTCCAGG ATTTCCAGGT CTTGAGCAGA ATCTGGACTC ACCCTTGGTG TTTGCAGCTG 
GACTATATTA GTAAAGAAAA TAAGGGTTAC TTTGATGAAG ACGACAGCAT GGAAGAGTTC 
ATAGCTTCCG ACTCTGAAGA ATCCTCCATG AGTCTGAGTT CTGAGGAAGA AGTTGTTAAT 
AAAAAGAAGG CCAAGGGAAA AGGCAAACGG GAGAACAGCT CAAGTGAAGA AGAAAGTGAC 
AATGATGTTG AAGTTATTAA GGTGTGGAAT TCAAGATCTC GAGGCGGCAA AGGAAAAGAT 
CTGAATGATG AAGAGGCCTC AAACAGCCCT GTAGTTCCTA AAGCTGAAGA AACAAAAGCA 
ACATCCTCTT CCACACCTGG AAGCCCAGCA GCTGACTGGT ACAAGGATTT TGTTACTGAA 
GCAGATTCAT CTATCATGGA GCATTCTGGA AAAATAATGC TTTTGTTTGA GATTTTGCGC 
ATGGCTGAGG TGTTGGGTGA CAAAGTTTTA GTTTTCAGCC AGTCTCTCAT ATCTCTGGAT 
TTGATAGAAG ATTTTCTGGA ATTGGCAAGT ACAGAGAAAA ATGAAGAAAA TGCATCTGAA 
AAGCCAGTAT TTTACAAAGG GGATGGGAAG TGGTTTAGAA ATATCGATTA CTATCGTTTA 
GACGGCCAAA CCTCCCCTCA AACACGTAAA AAGTGGGCAG AGGAGTTCAA TGATTCGACC 
AATATAAGGG GCCGTCTGTT CCTTATTTCT ACAAAAGCTG GATCCCTGGG TATTAATCTT 
GTTGCTGCTA ATCGTGTGAT AATCTTTGAT GCATCATGGA ATCCATCCTA TGATATCCAG 
AGTATCTTCA GGGTGTATCG ATTTGGACAG ATAAAGCCTG TATTTGTCTA CAGGTTTTTA 
GCCCAGGGCA CCATGGAAGA AAAGATATAT GACAGGCAGA TCGCCAAACA GTCCCTGTCC 
TTTAGAGTTA TTGATCAACA ACAAATAGAA CGTCACTTTA CAATGAATGA ACTGACTGAA 
CTTTACACCT TTGAGCCTGA TCTGCTGGAT GACCCTAATT CCGAAAAGAA GAAAAAGCGA 
GAGACTCCCA TGCTGCCTAA GGATACTGTG CTAGCAGAGC TCTTACAAAT CTTGAAGGAG 
TACATAGTCA CTTATCATGA GCACGACTCT CTTCTGGATC ATAAGGAGGA AGAGGCCCTT 
ACAGAAGAGG ATCGGAAGGC AGCATGGGCA GAATATGAAG CTGAAAAGAA GGGTTTGACC 
AATCGTTTTA ACGTGGCAGC AGTAACTGGG GCCAACTACG CTCAAGTCAA TATAAACTCG 
CAATCTTCAT ACATGCCTTT AAACTTGGGA GCGCTGTCTT CTTTATCCAA TCAGCAACTC 
GAGGATTTAA TAAATCAAGG CAGAGAAAAA GTGGTTGAAG CTACAAATAA CATAGCAACT 
GCAAGGATTC AACCCCTTGA AGACATTATC TCGACAGTGT GGAAGGAAAA TCCGACTGCC 
ACGGAGGCCC AAGTTCAGTC CCTTGCCCTA AGCAGGCAGT CGAGCCAAGA ATTGGAAATA 
AAGAGACGTG AGGCCATTTA TAACGACGTA CTGACTAAGC AACAGATGTT GATCAGCTGC 
GTGCAGCGAA TTCTCATGAG TCGAAGGTTA CAGCACCAAT ACAACCAGCA ACAGCAGCAG 
CAACAAATTG TTTTTCAACA GCAAGCAGCT CTGAATCGCC TGATGATGCA GAAAAATCCA 
AATATTATAA TATCCCCTTC TAACTTTTCT CAAATTGACA TGAGAGGAAT GTACCAAGCT 
GGGATGCAAG GTCATCAGAT GCAGCGTAAT GCACCCGCCT CTATAAGAGC AAAAAATCCA 
GGACCTTCCC AGGGAAAAAA AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931858.1
CDS	690..7496
Translation	MTAEPMSESKLNTLVKKLHDFLAHSSEESEDTTSPTRQSVSRSAAKVKSVHRLHPMENNMDEGSTSEKLKSLGSSRSKRKPSFVQKYVGSDEETVPDEVVNLDGSNENSENEVDMESLPIGTVVVQPEPVLNEDKDDFKGPEFRSRNKPKPDITIPKKRTEDGLHGIVNCTACGQQVNHFQKDSIYRHPALKVLICKACYKYYMSDDINRDADGMDEQCRWCAEGGNLICCDYCHNAFCKKCILRNLGRKELSAIMDEEMKWECYICRPEPLLDSVTACDSVFENLEQLLQQNKKKMKSDNEKNKSNEHSQNDSDKRNSSYNGQGKNVGDAHAGTLTYSYRALMVPKEMLKKAKKLIETTTSLNASFVKFMKQGTANNELNPAEKLRQLKAFKSVLGDLKKAHEALEETLMEEIHSVDEQKKSKKSKETTSENKHAKEKKLGEEKQKEPKKIVAREKAKPSDSDNKQRDCDKNASNDTEQAEEALIQNGKNVDLTEEDTIKSEPCSAAADVTEVLATDAEGTDISVVMEEADENVDGKNEKATVESDDNEGKQKSQKKKQKSMHDKPRENSPGNRKKSVDSPKKERESVLPEKTKEKKRADQCTESSKSGKSRTPQKKSKNAEATFEPNSTEALDMDIVSVPSSVPEDIFDSLDASLEQQNSLQDSVTSTAVSTPKCKEELKPLVKKQLIVQLTPVSLSNSPVKADSNSAGKSPKADIGSDAETEAQNQNSASCDEVLENSSENDLEESALRRSPRVKTTPRRRQTDANSAASNTEEESNDTDKEKKSKRAACSKEKRNSTAKHSPAESGESPKAKRSLAKKMQAQVNKSTSDSDEVPEVLKKAALMSNSSSELDSSNEANSGKRTSNRQDKTAKGYSKRKRSSSSSGSEPAKRTTRNSTAAKKKRGDQSDTSNSELEKEVKKLGKKDSVRKGLKQKSKKKEENSESSDEKNQNRSQKAKKSKRVRTPASNKKESSSDLSEEEQENSHNSDEDSDEQKIKPLVENEVVSATGFVQSSGDEAGTKCGAAPTEEDDDDDDDPENRIAKKMLLEEIKANLSSDDVEEASSDEEPEEGKKSVAKSTTKTEDKDVNEDDKSDSDSDSDSDSDSESEKKPRYRHHLLRRKISLSDGDSGEEKEKKDKAKNSKNGKRRNRRKVSSEESASDFHKSSGVSEEISESDDSDDQRPRTRAAKKEAEENQRSYKQKKKRRRIKVQDDSSDSKSNKGNSEDGDHDEDSDSKSPGKGRKKIRKIIKDDKLRTETQNALKEEEERRKRIAERERERDKLRELQVVDTEDVSPVKCPVTTKLVLDENEETKEALVQVHQKMVTKLKPHQVDGVQFMWDCCCESMKKTKKDPGSGCILAHCMGLGKTLQVVTFLHTVLLSEKLDFSTALVVCPLNTVLNWLNEFEKWQDGMDDEEKLEVCELATVKRPQERSYMMQRWHQEGGILIIGYEMYRNLTQGRNVKSKKLKEIFHKTLVDPGPDFVICDEGHILKNELSAISKAMNSIKTRRRIILTGTPLQNNLVEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQCADSTLVDVRVMKKRAHILYEMLAGCVQRRDYTALTKFLPPKHEYVLSVRISPLQCKLYQYYLDHLTGAVGEGGVRGKSGTKLFQDFQVLSRIWTHPWCLQLDYISKENKGYFDEDDSMEEFIASDSEESSMSLSSEEEVVNKKKAKGKGKRENSSSEEESDNDVEVIKVWNSRSRGGKGKDLNDEEASNSPVVPKAEETKATSSSTPGSPAADWYKDFVTEADSSIMEHSGKIMLLFEILRMAEVLGDKVLVFSQSLISLDLIEDFLELASTEKNEENASEKPVFYKGDGKWFRNIDYYRLDGQTSPQTRKKWAEEFNDSTNIRGRLFLISTKAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQIKPVFVYRFLAQGTMEEKIYDRQIAKQSLSFRVIDQQQIERHFTMNELTELYTFEPDLLDDPNSEKKKKRETPMLPKDTVLAELLQILKEYIVTYHEHDSLLDHKEEEALTEEDRKAAWAEYEAEKKGLTNRFNVAAVTGANYAQVNINSQSSYMPLNLGALSSLSNQQLEDLINQGREKVVEATNNIATARIQPLEDIISTVWKENPTATEAQVQSLALSRQSSQELEIKRREAIYNDVLTKQQMLISCVQRILMSRRLQHQYNQQQQQQQIVFQQQAALNRLMMQKNPNIIISPSNFSQIDMRGMYQAGMQGHQMQRNAPASIRAKNPGPSQGKKM

Target ORF information:

RefSeq Version	XM_002931812.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931812.4

ORF Insert Sequence:

ATGACAGCGG AGCCCATGAG TGAAAGCAAG TTAAATACAT TGGTGAAGAA ACTTCATGAT 
TTTCTAGCTC ACTCCTCAGA AGAATCTGAG GACACAACTT CTCCAACAAG ACAGTCTGTG 
AGCAGAAGTG CAGCTAAAGT CAAGTCTGTA CACCGACTGC ATCCAATGGA AAACAACATG 
GATGAGGGAA GTACTTCTGA AAAACTCAAG TCCTTGGGAT CATCTCGTTC AAAAAGGAAA 
CCCTCATTTG TCCAAAAATA TGTTGGGTCA GATGAAGAGA CTGTGCCAGA TGAAGTTGTC 
AATTTAGATG GGTCTAATGA GAATTCTGAG AATGAAGTTG ATATGGAAAG CTTGCCCATA 
GGTACAGTGG TTGTACAACC AGAACCTGTC TTAAACGAAG ATAAAGATGA TTTCAAAGGA 
CCAGAGTTTC GAAGCAGAAA CAAACCTAAA CCAGATATTA CTATACCTAA GAAACGCACA 
GAGGATGGAC TTCATGGTAT TGTTAACTGC ACAGCCTGTG GCCAACAAGT AAATCATTTT 
CAGAAGGATT CAATTTATCG ACATCCTGCT TTAAAAGTGC TGATATGTAA GGCATGCTAT 
AAATATTACA TGAGTGATGA CATAAACCGG GATGCAGACG GAATGGATGA ACAGTGCAGA 
TGGTGTGCTG AAGGAGGAAA CTTGATTTGC TGTGATTACT GTCATAATGC CTTTTGTAAG 
AAGTGCATTC TACGAAATCT AGGGAGAAAG GAACTGTCTG CTATTATGGA TGAAGAAATG 
AAGTGGGAGT GCTACATTTG CCGACCAGAA CCTTTACTGG ACTCTGTAAC TGCATGTGAC 
AGTGTGTTTG AAAATTTAGA GCAATTGTTA CAACAAAACA AGAAGAAAAT GAAATCAGAC 
AATGAAAAAA ACAAATCAAA TGAGCACTCC CAAAATGATT CTGATAAAAG GAATTCAAGC 
TACAATGGAC AGGGCAAAAA TGTAGGTGAT GCACATGCCG GTACTTTAAC GTATTCTTAT 
AGAGCCCTTA TGGTGCCAAA GGAGATGCTC AAAAAAGCGA AAAAGCTTAT TGAGACAACA 
ACTAGCCTGA ATGCAAGTTT TGTAAAGTTT ATGAAACAAG GAACTGCGAA CAATGAATTG 
AACCCTGCTG AAAAGTTAAG ACAGCTGAAA GCATTTAAAT CTGTCCTGGG TGACTTAAAG 
AAAGCCCATG AGGCTTTGGA GGAAACATTA ATGGAAGAGA TTCATTCAGT TGACGAGCAA 
AAAAAATCTA AGAAAAGCAA AGAGACTACG AGTGAGAATA AACATGCAAA GGAGAAGAAA 
CTTGGTGAAG AAAAGCAGAA AGAACCCAAG AAAATAGTTG CACGTGAAAA AGCAAAACCT 
TCAGATTCTG ACAATAAGCA AAGGGATTGT GACAAGAATG CCTCTAACGA TACAGAGCAG 
GCTGAAGAAG CGTTGATACA GAATGGGAAA AATGTAGATC TTACTGAAGA AGATACAATA 
AAGAGTGAAC CCTGTTCAGC TGCTGCTGAC GTTACAGAGG TTTTGGCAAC AGATGCAGAG 
GGTACAGATA TAAGTGTCGT CATGGAGGAG GCAGATGAAA ATGTTGATGG AAAAAATGAA 
AAAGCAACAG TTGAGAGTGA TGATAATGAA GGTAAGCAAA AATCCCAGAA AAAGAAGCAG 
AAGAGTATGC ATGACAAACC TAGGGAAAAC TCACCTGGTA ACAGAAAGAA GAGTGTAGAT 
TCACCTAAGA AAGAACGGGA GTCTGTGCTT CCTGAAAAGA CTAAAGAGAA GAAAAGGGCT 
GATCAGTGCA CTGAGTCTTC TAAATCTGGG AAGTCGCGCA CACCACAAAA GAAATCCAAA 
AATGCAGAGG CGACATTTGA GCCCAATTCC ACTGAAGCTC TTGATATGGA CATTGTTTCA 
GTCCCATCTT CTGTGCCAGA GGATATATTT GATAGCTTGG ATGCATCATT AGAGCAGCAA 
AATTCTTTGC AAGATTCTGT AACGAGTACT GCAGTATCTA CACCAAAGTG CAAGGAAGAA 
CTAAAGCCAC TGGTCAAGAA GCAGTTGATT GTGCAGCTGA CACCAGTGTC TCTATCCAAT 
TCCCCAGTGA AAGCAGACAG CAATAGTGCA GGCAAATCGC CAAAGGCTGA CATTGGTAGT 
GATGCTGAAA CGGAGGCGCA GAATCAAAAT AGTGCTTCAT GTGATGAAGT CCTTGAGAAC 
TCATCGGAGA ACGATCTAGA GGAATCTGCT CTCAGGAGAT CACCTCGCGT AAAAACTACT 
CCAAGGAGGC GACAAACTGA TGCAAATTCT GCAGCCTCAA ATACAGAAGA AGAGAGCAAT 
GACACAGACA AAGAGAAAAA AAGCAAAAGG GCAGCATGTT CTAAAGAAAA ACGGAATTCT 
ACAGCAAAGC ATAGCCCTGC AGAAAGTGGG GAGAGCCCCA AAGCAAAAAG ATCATTAGCA 
AAAAAGATGC AGGCACAAGT GAATAAGAGT ACTTCTGATT CAGATGAGGT TCCAGAAGTA 
CTTAAAAAGG CAGCCCTTAT GAGTAACAGC TCCTCAGAGC TTGATAGCAG TAATGAGGCA 
AACAGTGGTA AAAGGACTTC TAATCGTCAA GATAAAACAG CAAAAGGTTA TTCTAAGAGA 
AAAAGAAGTA GCTCAAGTTC TGGTTCTGAG CCAGCCAAAA GGACAACTAG AAACTCAACA 
GCTGCCAAAA AGAAACGTGG GGACCAATCG GACACTTCAA ATTCTGAGCT GGAGAAGGAA 
GTAAAAAAAC TCGGTAAAAA AGATTCTGTC AGGAAGGGCT TAAAGCAGAA ATCTAAGAAA 
AAGGAAGAAA ACAGTGAGTC CTCTGATGAA AAGAATCAAA ATAGATCCCA GAAAGCCAAA 
AAATCCAAAA GAGTGAGAAC ACCTGCCTCT AATAAGAAGG AATCCTCATC CGACCTTTCT 
GAAGAAGAGC AAGAAAATAG CCACAATTCA GATGAAGACA GCGATGAACA AAAGATCAAA 
CCATTAGTGG AAAATGAAGT TGTATCTGCC ACAGGATTTG TTCAATCATC AGGTGATGAA 
GCAGGTACAA AATGTGGGGC AGCTCCTACT GAGGAGGATG ATGACGATGA TGATGACCCT 
GAGAATAGGA TTGCCAAGAA AATGCTTCTG GAAGAAATCA AAGCCAATCT GTCTTCAGAT 
GATGTGGAAG AAGCATCCTC TGATGAAGAG CCAGAAGAAG GCAAAAAATC TGTGGCAAAG 
TCAACTACAA AAACTGAAGA TAAGGATGTT AATGAGGATG ATAAGTCCGA TTCAGATTCA 
GACTCTGACT CTGACTCTGA TTCAGAGTCT GAGAAAAAAC CAAGATATAG ACACCACCTT 
TTGCGCCGCA AGATAAGTTT AAGCGATGGA GATTCTGGAG AAGAAAAAGA GAAAAAAGAC 
AAAGCTAAGA ACTCAAAGAA CGGAAAGCGT CGAAACAGAC GGAAAGTGAG CAGTGAGGAA 
TCGGCCTCTG ACTTCCATAA ATCATCTGGG GTTAGCGAGG AAATTTCAGA GAGTGATGAT 
TCAGATGATC AGCGGCCCAG AACAAGAGCT GCAAAGAAAG AAGCCGAAGA GAACCAGCGT 
AGCTACAAGC AGAAAAAGAA GAGAAGGCGA ATTAAAGTCC AAGATGACTC CAGTGATAGC 
AAGAGTAATA AGGGTAATTC AGAGGATGGA GACCATGATG AGGACTCAGA CTCCAAATCA 
CCTGGTAAGG GCAGGAAGAA GATTAGGAAG ATTATTAAAG ATGACAAGTT GCGAACAGAA 
ACACAGAATG CCTTGAAAGA GGAAGAGGAG AGAAGGAAAA GAATAGCCGA GAGAGAGCGA 
GAACGAGACA AACTAAGAGA GTTACAGGTC GTTGATACTG AAGATGTCTC GCCTGTAAAG 
TGTCCAGTCA CTACTAAACT AGTGTTGGAT GAAAATGAAG AAACAAAAGA GGCTTTGGTG 
CAGGTTCACC AAAAGATGGT TACAAAGTTG AAACCGCATC AGGTAGACGG TGTTCAGTTC 
ATGTGGGACT GTTGCTGTGA GTCTATGAAA AAGACTAAAA AGGACCCTGG TTCTGGCTGT 
ATATTGGCTC ATTGTATGGG TCTTGGCAAG ACTTTACAGG TTGTGACTTT CCTGCACACT 
GTTCTTCTGT CTGAGAAGCT GGATTTCAGC ACTGCTCTTG TGGTGTGTCC TTTAAATACT 
GTTTTAAATT GGCTCAATGA ATTTGAGAAG TGGCAGGATG GCATGGACGA TGAAGAGAAA 
TTGGAGGTCT GTGAACTTGC TACAGTGAAG CGTCCTCAGG AAAGGTCATA CATGATGCAA 
CGCTGGCATC AAGAAGGAGG AATCTTAATA ATTGGATATG AAATGTACCG AAACCTTACT 
CAAGGGAGGA ATGTAAAAAG TAAAAAGTTG AAAGAGATTT TTCACAAGAC CCTTGTGGAT 
CCAGGACCTG ACTTTGTGAT ATGTGATGAA GGACACATCC TCAAAAATGA ATTGTCAGCC 
ATTTCTAAGG CCATGAATTC CATAAAGACA AGAAGAAGAA TCATACTTAC AGGAACCCCA 
CTGCAAAATA ATCTTGTAGA ATATCACTGC ATGGTCAACT TCATCAAGGA AAACCTACTT 
GGTTCCATTA AGGAATTCAG AAATCGTTTC ATCAATCCCA TCCAGAACGG ACAGTGCGCC 
GACTCTACCC TAGTTGATGT CAGAGTGATG AAAAAGAGAG CTCACATCTT GTATGAAATG 
TTAGCTGGCT GTGTTCAGCG AAGAGATTAT ACTGCACTAA CAAAGTTCTT GCCACCAAAG 
CATGAGTATG TATTGTCTGT CAGAATTTCC CCACTTCAGT GTAAGCTTTA CCAATACTAT 
CTGGACCACT TAACAGGAGC TGTTGGTGAA GGAGGTGTAA GAGGAAAATC TGGAACAAAA 
CTTTTCCAGG ATTTCCAGGT CTTGAGCAGA ATCTGGACTC ACCCTTGGTG TTTGCAGCTG 
GACTATATTA GTAAAGAAAA TAAGGGTTAC TTTGATGAAG ACGACAGCAT GGAAGAGTTC 
ATAGCTTCCG ACTCTGAAGA ATCCTCCATG AGTCTGAGTT CTGAGGAAGA AGTTGTTAAT 
AAAAAGAAGG CCAAGGGAAA AGGCAAACGG GAGAACAGCT CAAGTGAAGA AGAAAGTGAC 
AATGATGTTG AAGTTATTAA GGTGTGGAAT TCAAGATCTC GAGGCGGCAA AGGAAAAGAT 
CTGAATGATG AAGAGGCCTC AAACAGCCCT GTAGTTCCTA AAGCTGAAGA AACAAAAGCA 
ACATCCTCTT CCACACCTGG AAGCCCAGCA GCTGACTGGT ACAAGGATTT TGTTACTGAA 
GCAGATTCAT CTATCATGGA GCATTCTGGA AAAATAATGC TTTTGTTTGA GATTTTGCGC 
ATGGCTGAGG TGTTGGGTGA CAAAGTTTTA GTTTTCAGCC AGTCTCTCAT ATCTCTGGAT 
TTGATAGAAG ATTTTCTGGA ATTGGCAAGT ACAGAGAAAA ATGAAGAAAA TGCATCTGAA 
AAGCCAGTAT TTTACAAAGG GGATGGGAAG TGGTTTAGAA ATATCGATTA CTATCGTTTA 
GACGGCCAAA CCTCCCCTCA AACACGTAAA AAGTGGGCAG AGGAGTTCAA TGATTCGACC 
AATATAAGGG GCCGTCTGTT CCTTATTTCT ACAAAAGCTG GATCCCTGGG TATTAATCTT 
GTTGCTGCTA ATCGTGTGAT AATCTTTGAT GCATCATGGA ATCCATCCTA TGATATCCAG 
AGTATCTTCA GGGTGTATCG ATTTGGACAG ATAAAGCCTG TATTTGTCTA CAGGTTTTTA 
GCCCAGGGCA CCATGGAAGA AAAGATATAT GACAGGCAGA TCGCCAAACA GTCCCTGTCC 
TTTAGAGTTA TTGATCAACA ACAAATAGAA CGTCACTTTA CAATGAATGA ACTGACTGAA 
CTTTACACCT TTGAGCCTGA TCTGCTGGAT GACCCTAATT CCGAAAAGAA GAAAAAGCGA 
GAGACTCCCA TGCTGCCTAA GGATACTGTG CTAGCAGAGC TCTTACAAAT CTTGAAGGAG 
TACATAGTCA CTTATCATGA GCACGACTCT CTTCTGGATC ATAAGGAGGA AGAGGCCCTT 
ACAGAAGAGG ATCGGAAGGC AGCATGGGCA GAATATGAAG CTGAAAAGAA GGGTTTGACC 
AATCGTTTTA ACGTGGCAGC AGTAACTGGG GCCAACTACG CTCAAGTCAA TATAAACTCG 
CAATCTTCAT ACATGCCTTT AAACTTGGGA GCGCTGTCTT CTTTATCCAA TCAGCAACTC 
GAGGATTTAA TAAATCAAGG CAGAGAAAAA GTGGTTGAAG CTACAAATAA CATAGCAACT 
GCAAGGATTC AACCCCTTGA AGACATTATC TCGACAGTGT GGAAGGAAAA TCCGACTGCC 
ACGGAGGCCC AAGTTCAGTC CCTTGCCCTA AGCAGGCAGT CGAGCCAAGA ATTGGAAATA 
AAGAGACGTG AGGCCATTTA TAACGACGTA CTGACTAAGC AACAGATGTT GATCAGCTGC 
GTGCAGCGAA TTCTCATGAG TCGAAGGTTA CAGCACCAAT ACAACCAGCA ACAGCAGCAG 
CAACAAATTG TTTTTCAACA GCAAGCAGCT CTGAATCGCC TGATGATGCA GAAAAATCCA 
AATATTATAA TATCCCCTTC TAACTTTTCT CAAATTGACA TGAGAGGAAT GTACCAAGCT 
GGGATGCAAG GTCATCAGAT GCAGCGTAAT GCACCCGCCT CTATAAGAGC AAAAAATCCA 
GGACCTTCCC AGGGAAAAAA AATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atrx ( XM_004915741.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915798.1 Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X2, mRNA.

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CloneID	OXa12506
Clone ID Related Accession (Same CDS sequence)	XM_004915741.3
Accession Version	XM_004915741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional regulator ATRX isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915741.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCGG AGCCCATGAG TGAAAGCAAG TTAAATACAT TGGTGAAGAA ACTTCATGAT 
TTTCTAGCTC ACTCCTCAGA AGAATCTGAG GACACAACTT CTCCAACAAG ACAGTCTGTG 
AGCAGAAGTG CAGCTAAAGT CAAGTCTGTA CACCGACTGC ATCCAATGGA AAACAACATG 
GATGAGGGAA GTACTTCTGA AAAACTCAAG TCCTTGGGAT CATCTCGTTC AAAAAGGAAA 
CCCTCATTTG TCCAAAAATA TGTTGGGTCA GATGAAGAGA CTGTGCCAGA TGAAGTTGTC 
AATTTAGATG GGTCTAATGA GAATTCTGAG AATGAAGTTG ATATGGAAAG CTTGCCCATA 
GGTACAGTGG TTGTACAACC AGAACCTGTC TTAAACGAAG ATAAAGATGA TTTCAAAGGA 
CCAGAGTTTC GAAGCAGAAA CAAACCTAAA CCAGATATTA CTATACCTAA GAAACGCACA 
GAGGATGGAC TTCATGGTAT TGTTAACTGC ACAGCCTGTG GCCAACAAGT AAATCATTTT 
CAGAAGGATT CAATTTATCG ACATCCTGCT TTAAAAGTGC TGATATGTAA GGCATGCTAT 
AAATATTACA TGAGTGATGA CATAAACCGG GATGCAGACG GAATGGATGA ACAGTGCAGA 
TGGTGTGCTG AAGGAGGAAA CTTGATTTGC TGTGATTACT GTCATAATGC CTTTTGTAAG 
AAGTGCATTC TACGAAATCT AGGGAGAAAG GAACTGTCTG CTATTATGGA TGAAGAAATG 
AAGTGGGAGT GCTACATTTG CCGACCAGAA CCTTTACTGG ACTCTGTAAC TGCATGTGAC 
AGTGTGTTTG AAAATTTAGA GCAATTGTTA CAACAAAACA AGAAGAAAAT GAAATCAGAC 
AATGAAAAAA ACAAATCAAA TGAGCACTCC CAAAATGATT CTGATAAAAG GAATTCAAGC 
TACAATGGAC AGGGCAAAAA TGTAGGTGAT GCACATGCCG GTACTTTAAC GTATTCTTAT 
AGAGCCCTTA TGGTGCCAAA GGAGATGCTC AAAAAAGCGA AAAAGCTTAT TGAGACAACA 
ACTAGCCTGA ATGCAAGTTT TGTAAAGTTT ATGAAACAAG GAACTGCGAA CAATGAATTG 
AACCCTGCTG AAAAGTTAAG ACAGCTGAAA GCATTTAAAT CTGTCCTGGG TGACTTAAAG 
AAAGCCCATG AGGCTTTGGA GGAAACATTA ATGGAAGAGA TTCATTCAGT TGACGAGCAA 
AAAAAATCTA AGAAAAGCAA AGAGACTACG AGTGAGAATA AACATGCAAA GGAGAAGAAA 
CTTGGTGAAG AAAAGCAGAA AGAACCCAAG AAAATAGTTG CACGTGAAAA AGCAAAACCT 
TCAGATTCTG ACAATAAGCA AAGGGATTGT GACAAGAATG CCTCTAACGA TACAGAGCAG 
GCTGAAGAAG CGTTGATACA GAATGGGAAA AATGTAGATC TTACTGAAGA AGATACAATA 
AAGAGTGAAC CCTGTTCAGC TGCTGCTGAC GTTACAGAGG TTTTGGCAAC AGATGCAGAG 
GGTACAGATA TAAGTGTCGT CATGGAGGAG GCAGATGAAA ATGTTGATGG AAAAAATGAA 
AAAGCAACAG TTGAGAGTGA TGATAATGAA GGTAAGCAAA AATCCCAGAA AAAGAAGCAG 
AAGAGTATGC ATGACAAACC TAGGGAAAAC TCACCTGGTA ACAGAAAGAA GAGTGTAGAT 
TCACCTAAGA AAGAACGGGA GTCTGTGCTT CCTGAAAAGA CTAAAGAGAA GAAAAGGGCT 
GATCAGTGCA CTGAGTCTTC TAAATCTGGG AAGTCGCGCA CACCACAAAA GAAATCCAAA 
AATGCAGAGG CGACATTTGA GCCCAATTCC ACTGAAGCTC TTGATATGGA CATTGTTTCA 
GTCCCATCTT CTGTGCCAGA GGATATATTT GATAGCTTGG ATGCATCATT AGAGCAGCAA 
AATTCTTTGC AAGATTCTGT AACGAGTACT GCAGTATCTA CACCAAAGTG CAAGGAAGAA 
CTAAAGCCAC TGGTCAAGAA GCAGTTGATT GTGCAGCTGA CACCAGTGTC TCTATCCAAT 
TCCCCAGTGA AAGCAGACAG CAATAGTGCA GGCAAATCGC CAAAGGCTGA CATTGGTAGT 
GATGCTGAAA CGGAGGCGCA GAATCAAAAT AGTGCTTCAT GTGATGAAGT CCTTGAGAAC 
TCATCGGAGA ACGATCTAGA GGAATCTGCT CTCAGGAGAT CACCTCGCGT AAAAACTACT 
CCAAGGAGGC GACAAACTGA TGCAAATTCT GCAGCCTCAA ATACAGAAGA AGAGAGCAAT 
GACACAGACA AAGAGAAAAA AAGCAAAAGG GCAGCATGTT CTAAAGAAAA ACGGAATTCT 
ACAGCAAAGC ATAGCCCTGC AGAAAGTGGG GAGAGCCCCA AAGCAAAAAG ATCATTAGCA 
AAAAAGATGC AGGCACAAGT GAATAAGAGT ACTTCTGATT CAGATGAGGT TCCAGAAGTA 
CTTAAAAAGG CAGCCCTTAT GAGTAACAGC TCCTCAGAGC TTGATAGCAG TAATGAGGCA 
AACAGTGGTA AAAGGACTTC TAATCGTCAA GATAAAACAG CAAAAGGTTA TTCTAAGAGA 
AAAAGAAGTA GCTCAAGTTC TGGTTCTGAG CCAGCCAAAA GGACAACTAG AAACTCAACA 
GCTGCCAAAA AGAAACGTGG GGACCAATCG GACACTTCAA ATTCTGAGCT GGAGAAGGAA 
GTAAAAAAAC TCGGTAAAAA AGATTCTGTC AGGAAGGGCT TAAAGCAGAA ATCTAAGAAA 
AAGGAAGAAA ACAGTGAGTC CTCTGATGAA AAGAATCAAA ATAGATCCCA GAAAGCCAAA 
AAATCCAAAA GAGTGAGAAC ACCTGCCTCT AATAAGAAGG AATCCTCATC CGACCTTTCT 
GAAGAAGAGC AAGAAAATAG CCACAATTCA GATGAAGACA GCGATGAACA AAAGATCAAA 
CCATTAGTGG AAAATGAAGT TGTATCTGCC ACAGGATTTG TTCAATCATC AGGTGATGAA 
GCAGGTACAA AATGTGGGGC AGCTCCTACT GAGGAGGATG ATGACGATGA TGATGACCCT 
GAGAATAGGA TTGCCAAGAA AATGCTTCTG GAAGAAATCA AAGCCAATCT GTCTTCAGAT 
GATGTGGAAG AAGCATCCTC TGATGAAGAG CCAGAAGAAG GCAAAAAATC TGTGGCAAAG 
TCAACTACAA AAACTGAAGA TAAGGATGTT AATGAGGATG ATAAGTCCGA TTCAGATTCA 
GACTCTGACT CTGACTCTGA TTCAGAGTCT GAGAAAAAAC CAAGATATAG ACACCACCTT 
TTGCGCCGCA AGATAAGTTT AAGCGATGGA GATTCTGGAG AAGAAAAAGA GAAAAAAGAC 
AAAGCTAAGA ACTCAAAGAA CGGAAAGCGT CGAAACAGAC GGAAAGTGAG CAGTGAGGAA 
TCGGCCTCTG ACTTCCATAA ATCATCTGGG GTTAGCGAGG AAATTTCAGA GAGTGATGAT 
TCAGATGATC AGCGGCCCAG AACAAGAGCT GCAAAGAAAG AAGCCGAAGA GAACCAGCGT 
AGCTACAAGC AGAAAAAGAA GAGAAGGCGA ATTAAAGTCC AAGATGACTC CAGTGATAGC 
AAGAGTAATA AGGGTAATTC AGAGGATGGA GACCATGATG AGGACTCAGA CTCCAAATCA 
CCTGGTAAGG GCAGGAAGAA GATTAGGAAG ATTATTAAAG ATGACAAGTT GCGAACAGAA 
ACACAGAATG CCTTGAAAGA GGAAGAGGAG AGAAGGAAAA GAATAGCCGA GAGAGAGCGA 
GAACGAGACA AACTAAGAGA GGTCGTTGAT ACTGAAGATG TCTCGCCTGT AAAGTGTCCA 
GTCACTACTA AACTAGTGTT GGATGAAAAT GAAGAAACAA AAGAGGCTTT GGTGCAGGTT 
CACCAAAAGA TGGTTACAAA GTTGAAACCG CATCAGGTAG ACGGTGTTCA GTTCATGTGG 
GACTGTTGCT GTGAGTCTAT GAAAAAGACT AAAAAGGACC CTGGTTCTGG CTGTATATTG 
GCTCATTGTA TGGGTCTTGG CAAGACTTTA CAGGTTGTGA CTTTCCTGCA CACTGTTCTT 
CTGTCTGAGA AGCTGGATTT CAGCACTGCT CTTGTGGTGT GTCCTTTAAA TACTGTTTTA 
AATTGGCTCA ATGAATTTGA GAAGTGGCAG GATGGCATGG ACGATGAAGA GAAATTGGAG 
GTCTGTGAAC TTGCTACAGT GAAGCGTCCT CAGGAAAGGT CATACATGAT GCAACGCTGG 
CATCAAGAAG GAGGAATCTT AATAATTGGA TATGAAATGT ACCGAAACCT TACTCAAGGG 
AGGAATGTAA AAAGTAAAAA GTTGAAAGAG ATTTTTCACA AGACCCTTGT GGATCCAGGA 
CCTGACTTTG TGATATGTGA TGAAGGACAC ATCCTCAAAA ATGAATTGTC AGCCATTTCT 
AAGGCCATGA ATTCCATAAA GACAAGAAGA AGAATCATAC TTACAGGAAC CCCACTGCAA 
AATAATCTTG TAGAATATCA CTGCATGGTC AACTTCATCA AGGAAAACCT ACTTGGTTCC 
ATTAAGGAAT TCAGAAATCG TTTCATCAAT CCCATCCAGA ACGGACAGTG CGCCGACTCT 
ACCCTAGTTG ATGTCAGAGT GATGAAAAAG AGAGCTCACA TCTTGTATGA AATGTTAGCT 
GGCTGTGTTC AGCGAAGAGA TTATACTGCA CTAACAAAGT TCTTGCCACC AAAGCATGAG 
TATGTATTGT CTGTCAGAAT TTCCCCACTT CAGTGTAAGC TTTACCAATA CTATCTGGAC 
CACTTAACAG GAGCTGTTGG TGAAGGAGGT GTAAGAGGAA AATCTGGAAC AAAACTTTTC 
CAGGATTTCC AGGTCTTGAG CAGAATCTGG ACTCACCCTT GGTGTTTGCA GCTGGACTAT 
ATTAGTAAAG AAAATAAGGG TTACTTTGAT GAAGACGACA GCATGGAAGA GTTCATAGCT 
TCCGACTCTG AAGAATCCTC CATGAGTCTG AGTTCTGAGG AAGAAGTTGT TAATAAAAAG 
AAGGCCAAGG GAAAAGGCAA ACGGGAGAAC AGCTCAAGTG AAGAAGAAAG TGACAATGAT 
GTTGAAGTTA TTAAGGTGTG GAATTCAAGA TCTCGAGGCG GCAAAGGAAA AGATCTGAAT 
GATGAAGAGG CCTCAAACAG CCCTGTAGTT CCTAAAGCTG AAGAAACAAA AGCAACATCC 
TCTTCCACAC CTGGAAGCCC AGCAGCTGAC TGGTACAAGG ATTTTGTTAC TGAAGCAGAT 
TCATCTATCA TGGAGCATTC TGGAAAAATA ATGCTTTTGT TTGAGATTTT GCGCATGGCT 
GAGGTGTTGG GTGACAAAGT TTTAGTTTTC AGCCAGTCTC TCATATCTCT GGATTTGATA 
GAAGATTTTC TGGAATTGGC AAGTACAGAG AAAAATGAAG AAAATGCATC TGAAAAGCCA 
GTATTTTACA AAGGGGATGG GAAGTGGTTT AGAAATATCG ATTACTATCG TTTAGACGGC 
CAAACCTCCC CTCAAACACG TAAAAAGTGG GCAGAGGAGT TCAATGATTC GACCAATATA 
AGGGGCCGTC TGTTCCTTAT TTCTACAAAA GCTGGATCCC TGGGTATTAA TCTTGTTGCT 
GCTAATCGTG TGATAATCTT TGATGCATCA TGGAATCCAT CCTATGATAT CCAGAGTATC 
TTCAGGGTGT ATCGATTTGG ACAGATAAAG CCTGTATTTG TCTACAGGTT TTTAGCCCAG 
GGCACCATGG AAGAAAAGAT ATATGACAGG CAGATCGCCA AACAGTCCCT GTCCTTTAGA 
GTTATTGATC AACAACAAAT AGAACGTCAC TTTACAATGA ATGAACTGAC TGAACTTTAC 
ACCTTTGAGC CTGATCTGCT GGATGACCCT AATTCCGAAA AGAAGAAAAA GCGAGAGACT 
CCCATGCTGC CTAAGGATAC TGTGCTAGCA GAGCTCTTAC AAATCTTGAA GGAGTACATA 
GTCACTTATC ATGAGCACGA CTCTCTTCTG GATCATAAGG AGGAAGAGGC CCTTACAGAA 
GAGGATCGGA AGGCAGCATG GGCAGAATAT GAAGCTGAAA AGAAGGGTTT GACCAATCGT 
TTTAACGTGG CAGCAGTAAC TGGGGCCAAC TACGCTCAAG TCAATATAAA CTCGCAATCT 
TCATACATGC CTTTAAACTT GGGAGCGCTG TCTTCTTTAT CCAATCAGCA ACTCGAGGAT 
TTAATAAATC AAGGCAGAGA AAAAGTGGTT GAAGCTACAA ATAACATAGC AACTGCAAGG 
ATTCAACCCC TTGAAGACAT TATCTCGACA GTGTGGAAGG AAAATCCGAC TGCCACGGAG 
GCCCAAGTTC AGTCCCTTGC CCTAAGCAGG CAGTCGAGCC AAGAATTGGA AATAAAGAGA 
CGTGAGGCCA TTTATAACGA CGTACTGACT AAGCAACAGA TGTTGATCAG CTGCGTGCAG 
CGAATTCTCA TGAGTCGAAG GTTACAGCAC CAATACAACC AGCAACAGCA GCAGCAACAA 
ATTGTTTTTC AACAGCAAGC AGCTCTGAAT CGCCTGATGA TGCAGAAAAA TCCAAATATT 
ATAATATCCC CTTCTAACTT TTCTCAAATT GACATGAGAG GAATGTACCA AGCTGGGATG 
CAAGGTCATC AGATGCAGCG TAATGCACCC GCCTCTATAA GAGCAAAAAA TCCAGGACCT 
TCCCAGGGAA AAAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915798.1
CDS	386..7186
Translation	MTAEPMSESKLNTLVKKLHDFLAHSSEESEDTTSPTRQSVSRSAAKVKSVHRLHPMENNMDEGSTSEKLKSLGSSRSKRKPSFVQKYVGSDEETVPDEVVNLDGSNENSENEVDMESLPIGTVVVQPEPVLNEDKDDFKGPEFRSRNKPKPDITIPKKRTEDGLHGIVNCTACGQQVNHFQKDSIYRHPALKVLICKACYKYYMSDDINRDADGMDEQCRWCAEGGNLICCDYCHNAFCKKCILRNLGRKELSAIMDEEMKWECYICRPEPLLDSVTACDSVFENLEQLLQQNKKKMKSDNEKNKSNEHSQNDSDKRNSSYNGQGKNVGDAHAGTLTYSYRALMVPKEMLKKAKKLIETTTSLNASFVKFMKQGTANNELNPAEKLRQLKAFKSVLGDLKKAHEALEETLMEEIHSVDEQKKSKKSKETTSENKHAKEKKLGEEKQKEPKKIVAREKAKPSDSDNKQRDCDKNASNDTEQAEEALIQNGKNVDLTEEDTIKSEPCSAAADVTEVLATDAEGTDISVVMEEADENVDGKNEKATVESDDNEGKQKSQKKKQKSMHDKPRENSPGNRKKSVDSPKKERESVLPEKTKEKKRADQCTESSKSGKSRTPQKKSKNAEATFEPNSTEALDMDIVSVPSSVPEDIFDSLDASLEQQNSLQDSVTSTAVSTPKCKEELKPLVKKQLIVQLTPVSLSNSPVKADSNSAGKSPKADIGSDAETEAQNQNSASCDEVLENSSENDLEESALRRSPRVKTTPRRRQTDANSAASNTEEESNDTDKEKKSKRAACSKEKRNSTAKHSPAESGESPKAKRSLAKKMQAQVNKSTSDSDEVPEVLKKAALMSNSSSELDSSNEANSGKRTSNRQDKTAKGYSKRKRSSSSSGSEPAKRTTRNSTAAKKKRGDQSDTSNSELEKEVKKLGKKDSVRKGLKQKSKKKEENSESSDEKNQNRSQKAKKSKRVRTPASNKKESSSDLSEEEQENSHNSDEDSDEQKIKPLVENEVVSATGFVQSSGDEAGTKCGAAPTEEDDDDDDDPENRIAKKMLLEEIKANLSSDDVEEASSDEEPEEGKKSVAKSTTKTEDKDVNEDDKSDSDSDSDSDSDSESEKKPRYRHHLLRRKISLSDGDSGEEKEKKDKAKNSKNGKRRNRRKVSSEESASDFHKSSGVSEEISESDDSDDQRPRTRAAKKEAEENQRSYKQKKKRRRIKVQDDSSDSKSNKGNSEDGDHDEDSDSKSPGKGRKKIRKIIKDDKLRTETQNALKEEEERRKRIAERERERDKLREVVDTEDVSPVKCPVTTKLVLDENEETKEALVQVHQKMVTKLKPHQVDGVQFMWDCCCESMKKTKKDPGSGCILAHCMGLGKTLQVVTFLHTVLLSEKLDFSTALVVCPLNTVLNWLNEFEKWQDGMDDEEKLEVCELATVKRPQERSYMMQRWHQEGGILIIGYEMYRNLTQGRNVKSKKLKEIFHKTLVDPGPDFVICDEGHILKNELSAISKAMNSIKTRRRIILTGTPLQNNLVEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQCADSTLVDVRVMKKRAHILYEMLAGCVQRRDYTALTKFLPPKHEYVLSVRISPLQCKLYQYYLDHLTGAVGEGGVRGKSGTKLFQDFQVLSRIWTHPWCLQLDYISKENKGYFDEDDSMEEFIASDSEESSMSLSSEEEVVNKKKAKGKGKRENSSSEEESDNDVEVIKVWNSRSRGGKGKDLNDEEASNSPVVPKAEETKATSSSTPGSPAADWYKDFVTEADSSIMEHSGKIMLLFEILRMAEVLGDKVLVFSQSLISLDLIEDFLELASTEKNEENASEKPVFYKGDGKWFRNIDYYRLDGQTSPQTRKKWAEEFNDSTNIRGRLFLISTKAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQIKPVFVYRFLAQGTMEEKIYDRQIAKQSLSFRVIDQQQIERHFTMNELTELYTFEPDLLDDPNSEKKKKRETPMLPKDTVLAELLQILKEYIVTYHEHDSLLDHKEEEALTEEDRKAAWAEYEAEKKGLTNRFNVAAVTGANYAQVNINSQSSYMPLNLGALSSLSNQQLEDLINQGREKVVEATNNIATARIQPLEDIISTVWKENPTATEAQVQSLALSRQSSQELEIKRREAIYNDVLTKQQMLISCVQRILMSRRLQHQYNQQQQQQQIVFQQQAALNRLMMQKNPNIIISPSNFSQIDMRGMYQAGMQGHQMQRNAPASIRAKNPGPSQGKKM

Target ORF information:

RefSeq Version	XM_004915741.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915741.3

ORF Insert Sequence:

ATGACAGCGG AGCCCATGAG TGAAAGCAAG TTAAATACAT TGGTGAAGAA ACTTCATGAT 
TTTCTAGCTC ACTCCTCAGA AGAATCTGAG GACACAACTT CTCCAACAAG ACAGTCTGTG 
AGCAGAAGTG CAGCTAAAGT CAAGTCTGTA CACCGACTGC ATCCAATGGA AAACAACATG 
GATGAGGGAA GTACTTCTGA AAAACTCAAG TCCTTGGGAT CATCTCGTTC AAAAAGGAAA 
CCCTCATTTG TCCAAAAATA TGTTGGGTCA GATGAAGAGA CTGTGCCAGA TGAAGTTGTC 
AATTTAGATG GGTCTAATGA GAATTCTGAG AATGAAGTTG ATATGGAAAG CTTGCCCATA 
GGTACAGTGG TTGTACAACC AGAACCTGTC TTAAACGAAG ATAAAGATGA TTTCAAAGGA 
CCAGAGTTTC GAAGCAGAAA CAAACCTAAA CCAGATATTA CTATACCTAA GAAACGCACA 
GAGGATGGAC TTCATGGTAT TGTTAACTGC ACAGCCTGTG GCCAACAAGT AAATCATTTT 
CAGAAGGATT CAATTTATCG ACATCCTGCT TTAAAAGTGC TGATATGTAA GGCATGCTAT 
AAATATTACA TGAGTGATGA CATAAACCGG GATGCAGACG GAATGGATGA ACAGTGCAGA 
TGGTGTGCTG AAGGAGGAAA CTTGATTTGC TGTGATTACT GTCATAATGC CTTTTGTAAG 
AAGTGCATTC TACGAAATCT AGGGAGAAAG GAACTGTCTG CTATTATGGA TGAAGAAATG 
AAGTGGGAGT GCTACATTTG CCGACCAGAA CCTTTACTGG ACTCTGTAAC TGCATGTGAC 
AGTGTGTTTG AAAATTTAGA GCAATTGTTA CAACAAAACA AGAAGAAAAT GAAATCAGAC 
AATGAAAAAA ACAAATCAAA TGAGCACTCC CAAAATGATT CTGATAAAAG GAATTCAAGC 
TACAATGGAC AGGGCAAAAA TGTAGGTGAT GCACATGCCG GTACTTTAAC GTATTCTTAT 
AGAGCCCTTA TGGTGCCAAA GGAGATGCTC AAAAAAGCGA AAAAGCTTAT TGAGACAACA 
ACTAGCCTGA ATGCAAGTTT TGTAAAGTTT ATGAAACAAG GAACTGCGAA CAATGAATTG 
AACCCTGCTG AAAAGTTAAG ACAGCTGAAA GCATTTAAAT CTGTCCTGGG TGACTTAAAG 
AAAGCCCATG AGGCTTTGGA GGAAACATTA ATGGAAGAGA TTCATTCAGT TGACGAGCAA 
AAAAAATCTA AGAAAAGCAA AGAGACTACG AGTGAGAATA AACATGCAAA GGAGAAGAAA 
CTTGGTGAAG AAAAGCAGAA AGAACCCAAG AAAATAGTTG CACGTGAAAA AGCAAAACCT 
TCAGATTCTG ACAATAAGCA AAGGGATTGT GACAAGAATG CCTCTAACGA TACAGAGCAG 
GCTGAAGAAG CGTTGATACA GAATGGGAAA AATGTAGATC TTACTGAAGA AGATACAATA 
AAGAGTGAAC CCTGTTCAGC TGCTGCTGAC GTTACAGAGG TTTTGGCAAC AGATGCAGAG 
GGTACAGATA TAAGTGTCGT CATGGAGGAG GCAGATGAAA ATGTTGATGG AAAAAATGAA 
AAAGCAACAG TTGAGAGTGA TGATAATGAA GGTAAGCAAA AATCCCAGAA AAAGAAGCAG 
AAGAGTATGC ATGACAAACC TAGGGAAAAC TCACCTGGTA ACAGAAAGAA GAGTGTAGAT 
TCACCTAAGA AAGAACGGGA GTCTGTGCTT CCTGAAAAGA CTAAAGAGAA GAAAAGGGCT 
GATCAGTGCA CTGAGTCTTC TAAATCTGGG AAGTCGCGCA CACCACAAAA GAAATCCAAA 
AATGCAGAGG CGACATTTGA GCCCAATTCC ACTGAAGCTC TTGATATGGA CATTGTTTCA 
GTCCCATCTT CTGTGCCAGA GGATATATTT GATAGCTTGG ATGCATCATT AGAGCAGCAA 
AATTCTTTGC AAGATTCTGT AACGAGTACT GCAGTATCTA CACCAAAGTG CAAGGAAGAA 
CTAAAGCCAC TGGTCAAGAA GCAGTTGATT GTGCAGCTGA CACCAGTGTC TCTATCCAAT 
TCCCCAGTGA AAGCAGACAG CAATAGTGCA GGCAAATCGC CAAAGGCTGA CATTGGTAGT 
GATGCTGAAA CGGAGGCGCA GAATCAAAAT AGTGCTTCAT GTGATGAAGT CCTTGAGAAC 
TCATCGGAGA ACGATCTAGA GGAATCTGCT CTCAGGAGAT CACCTCGCGT AAAAACTACT 
CCAAGGAGGC GACAAACTGA TGCAAATTCT GCAGCCTCAA ATACAGAAGA AGAGAGCAAT 
GACACAGACA AAGAGAAAAA AAGCAAAAGG GCAGCATGTT CTAAAGAAAA ACGGAATTCT 
ACAGCAAAGC ATAGCCCTGC AGAAAGTGGG GAGAGCCCCA AAGCAAAAAG ATCATTAGCA 
AAAAAGATGC AGGCACAAGT GAATAAGAGT ACTTCTGATT CAGATGAGGT TCCAGAAGTA 
CTTAAAAAGG CAGCCCTTAT GAGTAACAGC TCCTCAGAGC TTGATAGCAG TAATGAGGCA 
AACAGTGGTA AAAGGACTTC TAATCGTCAA GATAAAACAG CAAAAGGTTA TTCTAAGAGA 
AAAAGAAGTA GCTCAAGTTC TGGTTCTGAG CCAGCCAAAA GGACAACTAG AAACTCAACA 
GCTGCCAAAA AGAAACGTGG GGACCAATCG GACACTTCAA ATTCTGAGCT GGAGAAGGAA 
GTAAAAAAAC TCGGTAAAAA AGATTCTGTC AGGAAGGGCT TAAAGCAGAA ATCTAAGAAA 
AAGGAAGAAA ACAGTGAGTC CTCTGATGAA AAGAATCAAA ATAGATCCCA GAAAGCCAAA 
AAATCCAAAA GAGTGAGAAC ACCTGCCTCT AATAAGAAGG AATCCTCATC CGACCTTTCT 
GAAGAAGAGC AAGAAAATAG CCACAATTCA GATGAAGACA GCGATGAACA AAAGATCAAA 
CCATTAGTGG AAAATGAAGT TGTATCTGCC ACAGGATTTG TTCAATCATC AGGTGATGAA 
GCAGGTACAA AATGTGGGGC AGCTCCTACT GAGGAGGATG ATGACGATGA TGATGACCCT 
GAGAATAGGA TTGCCAAGAA AATGCTTCTG GAAGAAATCA AAGCCAATCT GTCTTCAGAT 
GATGTGGAAG AAGCATCCTC TGATGAAGAG CCAGAAGAAG GCAAAAAATC TGTGGCAAAG 
TCAACTACAA AAACTGAAGA TAAGGATGTT AATGAGGATG ATAAGTCCGA TTCAGATTCA 
GACTCTGACT CTGACTCTGA TTCAGAGTCT GAGAAAAAAC CAAGATATAG ACACCACCTT 
TTGCGCCGCA AGATAAGTTT AAGCGATGGA GATTCTGGAG AAGAAAAAGA GAAAAAAGAC 
AAAGCTAAGA ACTCAAAGAA CGGAAAGCGT CGAAACAGAC GGAAAGTGAG CAGTGAGGAA 
TCGGCCTCTG ACTTCCATAA ATCATCTGGG GTTAGCGAGG AAATTTCAGA GAGTGATGAT 
TCAGATGATC AGCGGCCCAG AACAAGAGCT GCAAAGAAAG AAGCCGAAGA GAACCAGCGT 
AGCTACAAGC AGAAAAAGAA GAGAAGGCGA ATTAAAGTCC AAGATGACTC CAGTGATAGC 
AAGAGTAATA AGGGTAATTC AGAGGATGGA GACCATGATG AGGACTCAGA CTCCAAATCA 
CCTGGTAAGG GCAGGAAGAA GATTAGGAAG ATTATTAAAG ATGACAAGTT GCGAACAGAA 
ACACAGAATG CCTTGAAAGA GGAAGAGGAG AGAAGGAAAA GAATAGCCGA GAGAGAGCGA 
GAACGAGACA AACTAAGAGA GGTCGTTGAT ACTGAAGATG TCTCGCCTGT AAAGTGTCCA 
GTCACTACTA AACTAGTGTT GGATGAAAAT GAAGAAACAA AAGAGGCTTT GGTGCAGGTT 
CACCAAAAGA TGGTTACAAA GTTGAAACCG CATCAGGTAG ACGGTGTTCA GTTCATGTGG 
GACTGTTGCT GTGAGTCTAT GAAAAAGACT AAAAAGGACC CTGGTTCTGG CTGTATATTG 
GCTCATTGTA TGGGTCTTGG CAAGACTTTA CAGGTTGTGA CTTTCCTGCA CACTGTTCTT 
CTGTCTGAGA AGCTGGATTT CAGCACTGCT CTTGTGGTGT GTCCTTTAAA TACTGTTTTA 
AATTGGCTCA ATGAATTTGA GAAGTGGCAG GATGGCATGG ACGATGAAGA GAAATTGGAG 
GTCTGTGAAC TTGCTACAGT GAAGCGTCCT CAGGAAAGGT CATACATGAT GCAACGCTGG 
CATCAAGAAG GAGGAATCTT AATAATTGGA TATGAAATGT ACCGAAACCT TACTCAAGGG 
AGGAATGTAA AAAGTAAAAA GTTGAAAGAG ATTTTTCACA AGACCCTTGT GGATCCAGGA 
CCTGACTTTG TGATATGTGA TGAAGGACAC ATCCTCAAAA ATGAATTGTC AGCCATTTCT 
AAGGCCATGA ATTCCATAAA GACAAGAAGA AGAATCATAC TTACAGGAAC CCCACTGCAA 
AATAATCTTG TAGAATATCA CTGCATGGTC AACTTCATCA AGGAAAACCT ACTTGGTTCC 
ATTAAGGAAT TCAGAAATCG TTTCATCAAT CCCATCCAGA ACGGACAGTG CGCCGACTCT 
ACCCTAGTTG ATGTCAGAGT GATGAAAAAG AGAGCTCACA TCTTGTATGA AATGTTAGCT 
GGCTGTGTTC AGCGAAGAGA TTATACTGCA CTAACAAAGT TCTTGCCACC AAAGCATGAG 
TATGTATTGT CTGTCAGAAT TTCCCCACTT CAGTGTAAGC TTTACCAATA CTATCTGGAC 
CACTTAACAG GAGCTGTTGG TGAAGGAGGT GTAAGAGGAA AATCTGGAAC AAAACTTTTC 
CAGGATTTCC AGGTCTTGAG CAGAATCTGG ACTCACCCTT GGTGTTTGCA GCTGGACTAT 
ATTAGTAAAG AAAATAAGGG TTACTTTGAT GAAGACGACA GCATGGAAGA GTTCATAGCT 
TCCGACTCTG AAGAATCCTC CATGAGTCTG AGTTCTGAGG AAGAAGTTGT TAATAAAAAG 
AAGGCCAAGG GAAAAGGCAA ACGGGAGAAC AGCTCAAGTG AAGAAGAAAG TGACAATGAT 
GTTGAAGTTA TTAAGGTGTG GAATTCAAGA TCTCGAGGCG GCAAAGGAAA AGATCTGAAT 
GATGAAGAGG CCTCAAACAG CCCTGTAGTT CCTAAAGCTG AAGAAACAAA AGCAACATCC 
TCTTCCACAC CTGGAAGCCC AGCAGCTGAC TGGTACAAGG ATTTTGTTAC TGAAGCAGAT 
TCATCTATCA TGGAGCATTC TGGAAAAATA ATGCTTTTGT TTGAGATTTT GCGCATGGCT 
GAGGTGTTGG GTGACAAAGT TTTAGTTTTC AGCCAGTCTC TCATATCTCT GGATTTGATA 
GAAGATTTTC TGGAATTGGC AAGTACAGAG AAAAATGAAG AAAATGCATC TGAAAAGCCA 
GTATTTTACA AAGGGGATGG GAAGTGGTTT AGAAATATCG ATTACTATCG TTTAGACGGC 
CAAACCTCCC CTCAAACACG TAAAAAGTGG GCAGAGGAGT TCAATGATTC GACCAATATA 
AGGGGCCGTC TGTTCCTTAT TTCTACAAAA GCTGGATCCC TGGGTATTAA TCTTGTTGCT 
GCTAATCGTG TGATAATCTT TGATGCATCA TGGAATCCAT CCTATGATAT CCAGAGTATC 
TTCAGGGTGT ATCGATTTGG ACAGATAAAG CCTGTATTTG TCTACAGGTT TTTAGCCCAG 
GGCACCATGG AAGAAAAGAT ATATGACAGG CAGATCGCCA AACAGTCCCT GTCCTTTAGA 
GTTATTGATC AACAACAAAT AGAACGTCAC TTTACAATGA ATGAACTGAC TGAACTTTAC 
ACCTTTGAGC CTGATCTGCT GGATGACCCT AATTCCGAAA AGAAGAAAAA GCGAGAGACT 
CCCATGCTGC CTAAGGATAC TGTGCTAGCA GAGCTCTTAC AAATCTTGAA GGAGTACATA 
GTCACTTATC ATGAGCACGA CTCTCTTCTG GATCATAAGG AGGAAGAGGC CCTTACAGAA 
GAGGATCGGA AGGCAGCATG GGCAGAATAT GAAGCTGAAA AGAAGGGTTT GACCAATCGT 
TTTAACGTGG CAGCAGTAAC TGGGGCCAAC TACGCTCAAG TCAATATAAA CTCGCAATCT 
TCATACATGC CTTTAAACTT GGGAGCGCTG TCTTCTTTAT CCAATCAGCA ACTCGAGGAT 
TTAATAAATC AAGGCAGAGA AAAAGTGGTT GAAGCTACAA ATAACATAGC AACTGCAAGG 
ATTCAACCCC TTGAAGACAT TATCTCGACA GTGTGGAAGG AAAATCCGAC TGCCACGGAG 
GCCCAAGTTC AGTCCCTTGC CCTAAGCAGG CAGTCGAGCC AAGAATTGGA AATAAAGAGA 
CGTGAGGCCA TTTATAACGA CGTACTGACT AAGCAACAGA TGTTGATCAG CTGCGTGCAG 
CGAATTCTCA TGAGTCGAAG GTTACAGCAC CAATACAACC AGCAACAGCA GCAGCAACAA 
ATTGTTTTTC AACAGCAAGC AGCTCTGAAT CGCCTGATGA TGCAGAAAAA TCCAAATATT 
ATAATATCCC CTTCTAACTT TTCTCAAATT GACATGAGAG GAATGTACCA AGCTGGGATG 
CAAGGTCATC AGATGCAGCG TAATGCACCC GCCTCTATAA GAGCAAAAAA TCCAGGACCT 
TCCCAGGGAA AAAAAATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atrx ( XM_004915742.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915799.1 Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X3, mRNA.

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CloneID	OXa12507
Clone ID Related Accession (Same CDS sequence)	XM_004915742.3
Accession Version	XM_004915742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional regulator ATRX isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA ACATGGATGA GGGAAGTACT TCTGAAAAAC TCAAGTCCTT GGGATCATCT 
CGTTCAAAAA GGAAACCCTC ATTTGTCCAA AAATATGTTG GGTCAGATGA AGAGACTGTG 
CCAGATGAAG TTGTCAATTT AGATGGGTCT AATGAGAATT CTGAGAATGA AGTTGATATG 
GAAAGCTTGC CCATAGGTAC AGTGGTTGTA CAACCAGAAC CTGTCTTAAA CGAAGATAAA 
GATGATTTCA AAGGACCAGA GTTTCGAAGC AGAAACAAAC CTAAACCAGA TATTACTATA 
CCTAAGAAAC GCACAGAGGA TGGACTTCAT GGTATTGTTA ACTGCACAGC CTGTGGCCAA 
CAAGTAAATC ATTTTCAGAA GGATTCAATT TATCGACATC CTGCTTTAAA AGTGCTGATA 
TGTAAGGCAT GCTATAAATA TTACATGAGT GATGACATAA ACCGGGATGC AGACGGAATG 
GATGAACAGT GCAGATGGTG TGCTGAAGGA GGAAACTTGA TTTGCTGTGA TTACTGTCAT 
AATGCCTTTT GTAAGAAGTG CATTCTACGA AATCTAGGGA GAAAGGAACT GTCTGCTATT 
ATGGATGAAG AAATGAAGTG GGAGTGCTAC ATTTGCCGAC CAGAACCTTT ACTGGACTCT 
GTAACTGCAT GTGACAGTGT GTTTGAAAAT TTAGAGCAAT TGTTACAACA AAACAAGAAG 
AAAATGAAAT CAGACAATGA AAAAAACAAA TCAAATGAGC ACTCCCAAAA TGATTCTGAT 
AAAAGGAATT CAAGCTACAA TGGACAGGGC AAAAATGTAG GTGATGCACA TGCCGGTACT 
TTAACGTATT CTTATAGAGC CCTTATGGTG CCAAAGGAGA TGCTCAAAAA AGCGAAAAAG 
CTTATTGAGA CAACAACTAG CCTGAATGCA AGTTTTGTAA AGTTTATGAA ACAAGGAACT 
GCGAACAATG AATTGAACCC TGCTGAAAAG TTAAGACAGC TGAAAGCATT TAAATCTGTC 
CTGGGTGACT TAAAGAAAGC CCATGAGGCT TTGGAGGAAA CATTAATGGA AGAGATTCAT 
TCAGTTGACG AGCAAAAAAA ATCTAAGAAA AGCAAAGAGA CTACGAGTGA GAATAAACAT 
GCAAAGGAGA AGAAACTTGG TGAAGAAAAG CAGAAAGAAC CCAAGAAAAT AGTTGCACGT 
GAAAAAGCAA AACCTTCAGA TTCTGACAAT AAGCAAAGGG ATTGTGACAA GAATGCCTCT 
AACGATACAG AGCAGGCTGA AGAAGCGTTG ATACAGAATG GGAAAAATGT AGATCTTACT 
GAAGAAGATA CAATAAAGAG TGAACCCTGT TCAGCTGCTG CTGACGTTAC AGAGGTTTTG 
GCAACAGATG CAGAGGGTAC AGATATAAGT GTCGTCATGG AGGAGGCAGA TGAAAATGTT 
GATGGAAAAA ATGAAAAAGC AACAGTTGAG AGTGATGATA ATGAAGGTAA GCAAAAATCC 
CAGAAAAAGA AGCAGAAGAG TATGCATGAC AAACCTAGGG AAAACTCACC TGGTAACAGA 
AAGAAGAGTG TAGATTCACC TAAGAAAGAA CGGGAGTCTG TGCTTCCTGA AAAGACTAAA 
GAGAAGAAAA GGGCTGATCA GTGCACTGAG TCTTCTAAAT CTGGGAAGTC GCGCACACCA 
CAAAAGAAAT CCAAAAATGC AGAGGCGACA TTTGAGCCCA ATTCCACTGA AGCTCTTGAT 
ATGGACATTG TTTCAGTCCC ATCTTCTGTG CCAGAGGATA TATTTGATAG CTTGGATGCA 
TCATTAGAGC AGCAAAATTC TTTGCAAGAT TCTGTAACGA GTACTGCAGT ATCTACACCA 
AAGTGCAAGG AAGAACTAAA GCCACTGGTC AAGAAGCAGT TGATTGTGCA GCTGACACCA 
GTGTCTCTAT CCAATTCCCC AGTGAAAGCA GACAGCAATA GTGCAGGCAA ATCGCCAAAG 
GCTGACATTG GTAGTGATGC TGAAACGGAG GCGCAGAATC AAAATAGTGC TTCATGTGAT 
GAAGTCCTTG AGAACTCATC GGAGAACGAT CTAGAGGAAT CTGCTCTCAG GAGATCACCT 
CGCGTAAAAA CTACTCCAAG GAGGCGACAA ACTGATGCAA ATTCTGCAGC CTCAAATACA 
GAAGAAGAGA GCAATGACAC AGACAAAGAG AAAAAAAGCA AAAGGGCAGC ATGTTCTAAA 
GAAAAACGGA ATTCTACAGC AAAGCATAGC CCTGCAGAAA GTGGGGAGAG CCCCAAAGCA 
AAAAGATCAT TAGCAAAAAA GATGCAGGCA CAAGTGAATA AGAGTACTTC TGATTCAGAT 
GAGGTTCCAG AAGTACTTAA AAAGGCAGCC CTTATGAGTA ACAGCTCCTC AGAGCTTGAT 
AGCAGTAATG AGGCAAACAG TGGTAAAAGG ACTTCTAATC GTCAAGATAA AACAGCAAAA 
GGTTATTCTA AGAGAAAAAG AAGTAGCTCA AGTTCTGGTT CTGAGCCAGC CAAAAGGACA 
ACTAGAAACT CAACAGCTGC CAAAAAGAAA CGTGGGGACC AATCGGACAC TTCAAATTCT 
GAGCTGGAGA AGGAAGTAAA AAAACTCGGT AAAAAAGATT CTGTCAGGAA GGGCTTAAAG 
CAGAAATCTA AGAAAAAGGA AGAAAACAGT GAGTCCTCTG ATGAAAAGAA TCAAAATAGA 
TCCCAGAAAG CCAAAAAATC CAAAAGAGTG AGAACACCTG CCTCTAATAA GAAGGAATCC 
TCATCCGACC TTTCTGAAGA AGAGCAAGAA AATAGCCACA ATTCAGATGA AGACAGCGAT 
GAACAAAAGA TCAAACCATT AGTGGAAAAT GAAGTTGTAT CTGCCACAGG ATTTGTTCAA 
TCATCAGGTG ATGAAGCAGG TACAAAATGT GGGGCAGCTC CTACTGAGGA GGATGATGAC 
GATGATGATG ACCCTGAGAA TAGGATTGCC AAGAAAATGC TTCTGGAAGA AATCAAAGCC 
AATCTGTCTT CAGATGATGT GGAAGAAGCA TCCTCTGATG AAGAGCCAGA AGAAGGCAAA 
AAATCTGTGG CAAAGTCAAC TACAAAAACT GAAGATAAGG ATGTTAATGA GGATGATAAG 
TCCGATTCAG ATTCAGACTC TGACTCTGAC TCTGATTCAG AGTCTGAGAA AAAACCAAGA 
TATAGACACC ACCTTTTGCG CCGCAAGATA AGTTTAAGCG ATGGAGATTC TGGAGAAGAA 
AAAGAGAAAA AAGACAAAGC TAAGAACTCA AAGAACGGAA AGCGTCGAAA CAGACGGAAA 
GTGAGCAGTG AGGAATCGGC CTCTGACTTC CATAAATCAT CTGGGGTTAG CGAGGAAATT 
TCAGAGAGTG ATGATTCAGA TGATCAGCGG CCCAGAACAA GAGCTGCAAA GAAAGAAGCC 
GAAGAGAACC AGCGTAGCTA CAAGCAGAAA AAGAAGAGAA GGCGAATTAA AGTCCAAGAT 
GACTCCAGTG ATAGCAAGAG TAATAAGGGT AATTCAGAGG ATGGAGACCA TGATGAGGAC 
TCAGACTCCA AATCACCTGG TAAGGGCAGG AAGAAGATTA GGAAGATTAT TAAAGATGAC 
AAGTTGCGAA CAGAAACACA GAATGCCTTG AAAGAGGAAG AGGAGAGAAG GAAAAGAATA 
GCCGAGAGAG AGCGAGAACG AGACAAACTA AGAGAGTTAC AGGTCGTTGA TACTGAAGAT 
GTCTCGCCTG TAAAGTGTCC AGTCACTACT AAACTAGTGT TGGATGAAAA TGAAGAAACA 
AAAGAGGCTT TGGTGCAGGT TCACCAAAAG ATGGTTACAA AGTTGAAACC GCATCAGGTA 
GACGGTGTTC AGTTCATGTG GGACTGTTGC TGTGAGTCTA TGAAAAAGAC TAAAAAGGAC 
CCTGGTTCTG GCTGTATATT GGCTCATTGT ATGGGTCTTG GCAAGACTTT ACAGGTTGTG 
ACTTTCCTGC ACACTGTTCT TCTGTCTGAG AAGCTGGATT TCAGCACTGC TCTTGTGGTG 
TGTCCTTTAA ATACTGTTTT AAATTGGCTC AATGAATTTG AGAAGTGGCA GGATGGCATG 
GACGATGAAG AGAAATTGGA GGTCTGTGAA CTTGCTACAG TGAAGCGTCC TCAGGAAAGG 
TCATACATGA TGCAACGCTG GCATCAAGAA GGAGGAATCT TAATAATTGG ATATGAAATG 
TACCGAAACC TTACTCAAGG GAGGAATGTA AAAAGTAAAA AGTTGAAAGA GATTTTTCAC 
AAGACCCTTG TGGATCCAGG ACCTGACTTT GTGATATGTG ATGAAGGACA CATCCTCAAA 
AATGAATTGT CAGCCATTTC TAAGGCCATG AATTCCATAA AGACAAGAAG AAGAATCATA 
CTTACAGGAA CCCCACTGCA AAATAATCTT GTAGAATATC ACTGCATGGT CAACTTCATC 
AAGGAAAACC TACTTGGTTC CATTAAGGAA TTCAGAAATC GTTTCATCAA TCCCATCCAG 
AACGGACAGT GCGCCGACTC TACCCTAGTT GATGTCAGAG TGATGAAAAA GAGAGCTCAC 
ATCTTGTATG AAATGTTAGC TGGCTGTGTT CAGCGAAGAG ATTATACTGC ACTAACAAAG 
TTCTTGCCAC CAAAGCATGA GTATGTATTG TCTGTCAGAA TTTCCCCACT TCAGTGTAAG 
CTTTACCAAT ACTATCTGGA CCACTTAACA GGAGCTGTTG GTGAAGGAGG TGTAAGAGGA 
AAATCTGGAA CAAAACTTTT CCAGGATTTC CAGGTCTTGA GCAGAATCTG GACTCACCCT 
TGGTGTTTGC AGCTGGACTA TATTAGTAAA GAAAATAAGG GTTACTTTGA TGAAGACGAC 
AGCATGGAAG AGTTCATAGC TTCCGACTCT GAAGAATCCT CCATGAGTCT GAGTTCTGAG 
GAAGAAGTTG TTAATAAAAA GAAGGCCAAG GGAAAAGGCA AACGGGAGAA CAGCTCAAGT 
GAAGAAGAAA GTGACAATGA TGTTGAAGTT ATTAAGGTGT GGAATTCAAG ATCTCGAGGC 
GGCAAAGGAA AAGATCTGAA TGATGAAGAG GCCTCAAACA GCCCTGTAGT TCCTAAAGCT 
GAAGAAACAA AAGCAACATC CTCTTCCACA CCTGGAAGCC CAGCAGCTGA CTGGTACAAG 
GATTTTGTTA CTGAAGCAGA TTCATCTATC ATGGAGCATT CTGGAAAAAT AATGCTTTTG 
TTTGAGATTT TGCGCATGGC TGAGGTGTTG GGTGACAAAG TTTTAGTTTT CAGCCAGTCT 
CTCATATCTC TGGATTTGAT AGAAGATTTT CTGGAATTGG CAAGTACAGA GAAAAATGAA 
GAAAATGCAT CTGAAAAGCC AGTATTTTAC AAAGGGGATG GGAAGTGGTT TAGAAATATC 
GATTACTATC GTTTAGACGG CCAAACCTCC CCTCAAACAC GTAAAAAGTG GGCAGAGGAG 
TTCAATGATT CGACCAATAT AAGGGGCCGT CTGTTCCTTA TTTCTACAAA AGCTGGATCC 
CTGGGTATTA ATCTTGTTGC TGCTAATCGT GTGATAATCT TTGATGCATC ATGGAATCCA 
TCCTATGATA TCCAGAGTAT CTTCAGGGTG TATCGATTTG GACAGATAAA GCCTGTATTT 
GTCTACAGGT TTTTAGCCCA GGGCACCATG GAAGAAAAGA TATATGACAG GCAGATCGCC 
AAACAGTCCC TGTCCTTTAG AGTTATTGAT CAACAACAAA TAGAACGTCA CTTTACAATG 
AATGAACTGA CTGAACTTTA CACCTTTGAG CCTGATCTGC TGGATGACCC TAATTCCGAA 
AAGAAGAAAA AGCGAGAGAC TCCCATGCTG CCTAAGGATA CTGTGCTAGC AGAGCTCTTA 
CAAATCTTGA AGGAGTACAT AGTCACTTAT CATGAGCACG ACTCTCTTCT GGATCATAAG 
GAGGAAGAGG CCCTTACAGA AGAGGATCGG AAGGCAGCAT GGGCAGAATA TGAAGCTGAA 
AAGAAGGGTT TGACCAATCG TTTTAACGTG GCAGCAGTAA CTGGGGCCAA CTACGCTCAA 
GTCAATATAA ACTCGCAATC TTCATACATG CCTTTAAACT TGGGAGCGCT GTCTTCTTTA 
TCCAATCAGC AACTCGAGGA TTTAATAAAT CAAGGCAGAG AAAAAGTGGT TGAAGCTACA 
AATAACATAG CAACTGCAAG GATTCAACCC CTTGAAGACA TTATCTCGAC AGTGTGGAAG 
GAAAATCCGA CTGCCACGGA GGCCCAAGTT CAGTCCCTTG CCCTAAGCAG GCAGTCGAGC 
CAAGAATTGG AAATAAAGAG ACGTGAGGCC ATTTATAACG ACGTACTGAC TAAGCAACAG 
ATGTTGATCA GCTGCGTGCA GCGAATTCTC ATGAGTCGAA GGTTACAGCA CCAATACAAC 
CAGCAACAGC AGCAGCAACA AATTGTTTTT CAACAGCAAG CAGCTCTGAA TCGCCTGATG 
ATGCAGAAAA ATCCAAATAT TATAATATCC CCTTCTAACT TTTCTCAAAT TGACATGAGA 
GGAATGTACC AAGCTGGGAT GCAAGGTCAT CAGATGCAGC GTAATGCACC CGCCTCTATA 
AGAGCAAAAA ATCCAGGACC TTCCCAGGGA AAAAAAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915799.1
CDS	1010..7651
Translation	MENNMDEGSTSEKLKSLGSSRSKRKPSFVQKYVGSDEETVPDEVVNLDGSNENSENEVDMESLPIGTVVVQPEPVLNEDKDDFKGPEFRSRNKPKPDITIPKKRTEDGLHGIVNCTACGQQVNHFQKDSIYRHPALKVLICKACYKYYMSDDINRDADGMDEQCRWCAEGGNLICCDYCHNAFCKKCILRNLGRKELSAIMDEEMKWECYICRPEPLLDSVTACDSVFENLEQLLQQNKKKMKSDNEKNKSNEHSQNDSDKRNSSYNGQGKNVGDAHAGTLTYSYRALMVPKEMLKKAKKLIETTTSLNASFVKFMKQGTANNELNPAEKLRQLKAFKSVLGDLKKAHEALEETLMEEIHSVDEQKKSKKSKETTSENKHAKEKKLGEEKQKEPKKIVAREKAKPSDSDNKQRDCDKNASNDTEQAEEALIQNGKNVDLTEEDTIKSEPCSAAADVTEVLATDAEGTDISVVMEEADENVDGKNEKATVESDDNEGKQKSQKKKQKSMHDKPRENSPGNRKKSVDSPKKERESVLPEKTKEKKRADQCTESSKSGKSRTPQKKSKNAEATFEPNSTEALDMDIVSVPSSVPEDIFDSLDASLEQQNSLQDSVTSTAVSTPKCKEELKPLVKKQLIVQLTPVSLSNSPVKADSNSAGKSPKADIGSDAETEAQNQNSASCDEVLENSSENDLEESALRRSPRVKTTPRRRQTDANSAASNTEEESNDTDKEKKSKRAACSKEKRNSTAKHSPAESGESPKAKRSLAKKMQAQVNKSTSDSDEVPEVLKKAALMSNSSSELDSSNEANSGKRTSNRQDKTAKGYSKRKRSSSSSGSEPAKRTTRNSTAAKKKRGDQSDTSNSELEKEVKKLGKKDSVRKGLKQKSKKKEENSESSDEKNQNRSQKAKKSKRVRTPASNKKESSSDLSEEEQENSHNSDEDSDEQKIKPLVENEVVSATGFVQSSGDEAGTKCGAAPTEEDDDDDDDPENRIAKKMLLEEIKANLSSDDVEEASSDEEPEEGKKSVAKSTTKTEDKDVNEDDKSDSDSDSDSDSDSESEKKPRYRHHLLRRKISLSDGDSGEEKEKKDKAKNSKNGKRRNRRKVSSEESASDFHKSSGVSEEISESDDSDDQRPRTRAAKKEAEENQRSYKQKKKRRRIKVQDDSSDSKSNKGNSEDGDHDEDSDSKSPGKGRKKIRKIIKDDKLRTETQNALKEEEERRKRIAERERERDKLRELQVVDTEDVSPVKCPVTTKLVLDENEETKEALVQVHQKMVTKLKPHQVDGVQFMWDCCCESMKKTKKDPGSGCILAHCMGLGKTLQVVTFLHTVLLSEKLDFSTALVVCPLNTVLNWLNEFEKWQDGMDDEEKLEVCELATVKRPQERSYMMQRWHQEGGILIIGYEMYRNLTQGRNVKSKKLKEIFHKTLVDPGPDFVICDEGHILKNELSAISKAMNSIKTRRRIILTGTPLQNNLVEYHCMVNFIKENLLGSIKEFRNRFINPIQNGQCADSTLVDVRVMKKRAHILYEMLAGCVQRRDYTALTKFLPPKHEYVLSVRISPLQCKLYQYYLDHLTGAVGEGGVRGKSGTKLFQDFQVLSRIWTHPWCLQLDYISKENKGYFDEDDSMEEFIASDSEESSMSLSSEEEVVNKKKAKGKGKRENSSSEEESDNDVEVIKVWNSRSRGGKGKDLNDEEASNSPVVPKAEETKATSSSTPGSPAADWYKDFVTEADSSIMEHSGKIMLLFEILRMAEVLGDKVLVFSQSLISLDLIEDFLELASTEKNEENASEKPVFYKGDGKWFRNIDYYRLDGQTSPQTRKKWAEEFNDSTNIRGRLFLISTKAGSLGINLVAANRVIIFDASWNPSYDIQSIFRVYRFGQIKPVFVYRFLAQGTMEEKIYDRQIAKQSLSFRVIDQQQIERHFTMNELTELYTFEPDLLDDPNSEKKKKRETPMLPKDTVLAELLQILKEYIVTYHEHDSLLDHKEEEALTEEDRKAAWAEYEAEKKGLTNRFNVAAVTGANYAQVNINSQSSYMPLNLGALSSLSNQQLEDLINQGREKVVEATNNIATARIQPLEDIISTVWKENPTATEAQVQSLALSRQSSQELEIKRREAIYNDVLTKQQMLISCVQRILMSRRLQHQYNQQQQQQQIVFQQQAALNRLMMQKNPNIIISPSNFSQIDMRGMYQAGMQGHQMQRNAPASIRAKNPGPSQGKKM

Target ORF information:

RefSeq Version	XM_004915742.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alpha thalassemia/mental retardation syndrome X-linked (atrx), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915742.3

ORF Insert Sequence:

ATGGAAAACA ACATGGATGA GGGAAGTACT TCTGAAAAAC TCAAGTCCTT GGGATCATCT 
CGTTCAAAAA GGAAACCCTC ATTTGTCCAA AAATATGTTG GGTCAGATGA AGAGACTGTG 
CCAGATGAAG TTGTCAATTT AGATGGGTCT AATGAGAATT CTGAGAATGA AGTTGATATG 
GAAAGCTTGC CCATAGGTAC AGTGGTTGTA CAACCAGAAC CTGTCTTAAA CGAAGATAAA 
GATGATTTCA AAGGACCAGA GTTTCGAAGC AGAAACAAAC CTAAACCAGA TATTACTATA 
CCTAAGAAAC GCACAGAGGA TGGACTTCAT GGTATTGTTA ACTGCACAGC CTGTGGCCAA 
CAAGTAAATC ATTTTCAGAA GGATTCAATT TATCGACATC CTGCTTTAAA AGTGCTGATA 
TGTAAGGCAT GCTATAAATA TTACATGAGT GATGACATAA ACCGGGATGC AGACGGAATG 
GATGAACAGT GCAGATGGTG TGCTGAAGGA GGAAACTTGA TTTGCTGTGA TTACTGTCAT 
AATGCCTTTT GTAAGAAGTG CATTCTACGA AATCTAGGGA GAAAGGAACT GTCTGCTATT 
ATGGATGAAG AAATGAAGTG GGAGTGCTAC ATTTGCCGAC CAGAACCTTT ACTGGACTCT 
GTAACTGCAT GTGACAGTGT GTTTGAAAAT TTAGAGCAAT TGTTACAACA AAACAAGAAG 
AAAATGAAAT CAGACAATGA AAAAAACAAA TCAAATGAGC ACTCCCAAAA TGATTCTGAT 
AAAAGGAATT CAAGCTACAA TGGACAGGGC AAAAATGTAG GTGATGCACA TGCCGGTACT 
TTAACGTATT CTTATAGAGC CCTTATGGTG CCAAAGGAGA TGCTCAAAAA AGCGAAAAAG 
CTTATTGAGA CAACAACTAG CCTGAATGCA AGTTTTGTAA AGTTTATGAA ACAAGGAACT 
GCGAACAATG AATTGAACCC TGCTGAAAAG TTAAGACAGC TGAAAGCATT TAAATCTGTC 
CTGGGTGACT TAAAGAAAGC CCATGAGGCT TTGGAGGAAA CATTAATGGA AGAGATTCAT 
TCAGTTGACG AGCAAAAAAA ATCTAAGAAA AGCAAAGAGA CTACGAGTGA GAATAAACAT 
GCAAAGGAGA AGAAACTTGG TGAAGAAAAG CAGAAAGAAC CCAAGAAAAT AGTTGCACGT 
GAAAAAGCAA AACCTTCAGA TTCTGACAAT AAGCAAAGGG ATTGTGACAA GAATGCCTCT 
AACGATACAG AGCAGGCTGA AGAAGCGTTG ATACAGAATG GGAAAAATGT AGATCTTACT 
GAAGAAGATA CAATAAAGAG TGAACCCTGT TCAGCTGCTG CTGACGTTAC AGAGGTTTTG 
GCAACAGATG CAGAGGGTAC AGATATAAGT GTCGTCATGG AGGAGGCAGA TGAAAATGTT 
GATGGAAAAA ATGAAAAAGC AACAGTTGAG AGTGATGATA ATGAAGGTAA GCAAAAATCC 
CAGAAAAAGA AGCAGAAGAG TATGCATGAC AAACCTAGGG AAAACTCACC TGGTAACAGA 
AAGAAGAGTG TAGATTCACC TAAGAAAGAA CGGGAGTCTG TGCTTCCTGA AAAGACTAAA 
GAGAAGAAAA GGGCTGATCA GTGCACTGAG TCTTCTAAAT CTGGGAAGTC GCGCACACCA 
CAAAAGAAAT CCAAAAATGC AGAGGCGACA TTTGAGCCCA ATTCCACTGA AGCTCTTGAT 
ATGGACATTG TTTCAGTCCC ATCTTCTGTG CCAGAGGATA TATTTGATAG CTTGGATGCA 
TCATTAGAGC AGCAAAATTC TTTGCAAGAT TCTGTAACGA GTACTGCAGT ATCTACACCA 
AAGTGCAAGG AAGAACTAAA GCCACTGGTC AAGAAGCAGT TGATTGTGCA GCTGACACCA 
GTGTCTCTAT CCAATTCCCC AGTGAAAGCA GACAGCAATA GTGCAGGCAA ATCGCCAAAG 
GCTGACATTG GTAGTGATGC TGAAACGGAG GCGCAGAATC AAAATAGTGC TTCATGTGAT 
GAAGTCCTTG AGAACTCATC GGAGAACGAT CTAGAGGAAT CTGCTCTCAG GAGATCACCT 
CGCGTAAAAA CTACTCCAAG GAGGCGACAA ACTGATGCAA ATTCTGCAGC CTCAAATACA 
GAAGAAGAGA GCAATGACAC AGACAAAGAG AAAAAAAGCA AAAGGGCAGC ATGTTCTAAA 
GAAAAACGGA ATTCTACAGC AAAGCATAGC CCTGCAGAAA GTGGGGAGAG CCCCAAAGCA 
AAAAGATCAT TAGCAAAAAA GATGCAGGCA CAAGTGAATA AGAGTACTTC TGATTCAGAT 
GAGGTTCCAG AAGTACTTAA AAAGGCAGCC CTTATGAGTA ACAGCTCCTC AGAGCTTGAT 
AGCAGTAATG AGGCAAACAG TGGTAAAAGG ACTTCTAATC GTCAAGATAA AACAGCAAAA 
GGTTATTCTA AGAGAAAAAG AAGTAGCTCA AGTTCTGGTT CTGAGCCAGC CAAAAGGACA 
ACTAGAAACT CAACAGCTGC CAAAAAGAAA CGTGGGGACC AATCGGACAC TTCAAATTCT 
GAGCTGGAGA AGGAAGTAAA AAAACTCGGT AAAAAAGATT CTGTCAGGAA GGGCTTAAAG 
CAGAAATCTA AGAAAAAGGA AGAAAACAGT GAGTCCTCTG ATGAAAAGAA TCAAAATAGA 
TCCCAGAAAG CCAAAAAATC CAAAAGAGTG AGAACACCTG CCTCTAATAA GAAGGAATCC 
TCATCCGACC TTTCTGAAGA AGAGCAAGAA AATAGCCACA ATTCAGATGA AGACAGCGAT 
GAACAAAAGA TCAAACCATT AGTGGAAAAT GAAGTTGTAT CTGCCACAGG ATTTGTTCAA 
TCATCAGGTG ATGAAGCAGG TACAAAATGT GGGGCAGCTC CTACTGAGGA GGATGATGAC 
GATGATGATG ACCCTGAGAA TAGGATTGCC AAGAAAATGC TTCTGGAAGA AATCAAAGCC 
AATCTGTCTT CAGATGATGT GGAAGAAGCA TCCTCTGATG AAGAGCCAGA AGAAGGCAAA 
AAATCTGTGG CAAAGTCAAC TACAAAAACT GAAGATAAGG ATGTTAATGA GGATGATAAG 
TCCGATTCAG ATTCAGACTC TGACTCTGAC TCTGATTCAG AGTCTGAGAA AAAACCAAGA 
TATAGACACC ACCTTTTGCG CCGCAAGATA AGTTTAAGCG ATGGAGATTC TGGAGAAGAA 
AAAGAGAAAA AAGACAAAGC TAAGAACTCA AAGAACGGAA AGCGTCGAAA CAGACGGAAA 
GTGAGCAGTG AGGAATCGGC CTCTGACTTC CATAAATCAT CTGGGGTTAG CGAGGAAATT 
TCAGAGAGTG ATGATTCAGA TGATCAGCGG CCCAGAACAA GAGCTGCAAA GAAAGAAGCC 
GAAGAGAACC AGCGTAGCTA CAAGCAGAAA AAGAAGAGAA GGCGAATTAA AGTCCAAGAT 
GACTCCAGTG ATAGCAAGAG TAATAAGGGT AATTCAGAGG ATGGAGACCA TGATGAGGAC 
TCAGACTCCA AATCACCTGG TAAGGGCAGG AAGAAGATTA GGAAGATTAT TAAAGATGAC 
AAGTTGCGAA CAGAAACACA GAATGCCTTG AAAGAGGAAG AGGAGAGAAG GAAAAGAATA 
GCCGAGAGAG AGCGAGAACG AGACAAACTA AGAGAGTTAC AGGTCGTTGA TACTGAAGAT 
GTCTCGCCTG TAAAGTGTCC AGTCACTACT AAACTAGTGT TGGATGAAAA TGAAGAAACA 
AAAGAGGCTT TGGTGCAGGT TCACCAAAAG ATGGTTACAA AGTTGAAACC GCATCAGGTA 
GACGGTGTTC AGTTCATGTG GGACTGTTGC TGTGAGTCTA TGAAAAAGAC TAAAAAGGAC 
CCTGGTTCTG GCTGTATATT GGCTCATTGT ATGGGTCTTG GCAAGACTTT ACAGGTTGTG 
ACTTTCCTGC ACACTGTTCT TCTGTCTGAG AAGCTGGATT TCAGCACTGC TCTTGTGGTG 
TGTCCTTTAA ATACTGTTTT AAATTGGCTC AATGAATTTG AGAAGTGGCA GGATGGCATG 
GACGATGAAG AGAAATTGGA GGTCTGTGAA CTTGCTACAG TGAAGCGTCC TCAGGAAAGG 
TCATACATGA TGCAACGCTG GCATCAAGAA GGAGGAATCT TAATAATTGG ATATGAAATG 
TACCGAAACC TTACTCAAGG GAGGAATGTA AAAAGTAAAA AGTTGAAAGA GATTTTTCAC 
AAGACCCTTG TGGATCCAGG ACCTGACTTT GTGATATGTG ATGAAGGACA CATCCTCAAA 
AATGAATTGT CAGCCATTTC TAAGGCCATG AATTCCATAA AGACAAGAAG AAGAATCATA 
CTTACAGGAA CCCCACTGCA AAATAATCTT GTAGAATATC ACTGCATGGT CAACTTCATC 
AAGGAAAACC TACTTGGTTC CATTAAGGAA TTCAGAAATC GTTTCATCAA TCCCATCCAG 
AACGGACAGT GCGCCGACTC TACCCTAGTT GATGTCAGAG TGATGAAAAA GAGAGCTCAC 
ATCTTGTATG AAATGTTAGC TGGCTGTGTT CAGCGAAGAG ATTATACTGC ACTAACAAAG 
TTCTTGCCAC CAAAGCATGA GTATGTATTG TCTGTCAGAA TTTCCCCACT TCAGTGTAAG 
CTTTACCAAT ACTATCTGGA CCACTTAACA GGAGCTGTTG GTGAAGGAGG TGTAAGAGGA 
AAATCTGGAA CAAAACTTTT CCAGGATTTC CAGGTCTTGA GCAGAATCTG GACTCACCCT 
TGGTGTTTGC AGCTGGACTA TATTAGTAAA GAAAATAAGG GTTACTTTGA TGAAGACGAC 
AGCATGGAAG AGTTCATAGC TTCCGACTCT GAAGAATCCT CCATGAGTCT GAGTTCTGAG 
GAAGAAGTTG TTAATAAAAA GAAGGCCAAG GGAAAAGGCA AACGGGAGAA CAGCTCAAGT 
GAAGAAGAAA GTGACAATGA TGTTGAAGTT ATTAAGGTGT GGAATTCAAG ATCTCGAGGC 
GGCAAAGGAA AAGATCTGAA TGATGAAGAG GCCTCAAACA GCCCTGTAGT TCCTAAAGCT 
GAAGAAACAA AAGCAACATC CTCTTCCACA CCTGGAAGCC CAGCAGCTGA CTGGTACAAG 
GATTTTGTTA CTGAAGCAGA TTCATCTATC ATGGAGCATT CTGGAAAAAT AATGCTTTTG 
TTTGAGATTT TGCGCATGGC TGAGGTGTTG GGTGACAAAG TTTTAGTTTT CAGCCAGTCT 
CTCATATCTC TGGATTTGAT AGAAGATTTT CTGGAATTGG CAAGTACAGA GAAAAATGAA 
GAAAATGCAT CTGAAAAGCC AGTATTTTAC AAAGGGGATG GGAAGTGGTT TAGAAATATC 
GATTACTATC GTTTAGACGG CCAAACCTCC CCTCAAACAC GTAAAAAGTG GGCAGAGGAG 
TTCAATGATT CGACCAATAT AAGGGGCCGT CTGTTCCTTA TTTCTACAAA AGCTGGATCC 
CTGGGTATTA ATCTTGTTGC TGCTAATCGT GTGATAATCT TTGATGCATC ATGGAATCCA 
TCCTATGATA TCCAGAGTAT CTTCAGGGTG TATCGATTTG GACAGATAAA GCCTGTATTT 
GTCTACAGGT TTTTAGCCCA GGGCACCATG GAAGAAAAGA TATATGACAG GCAGATCGCC 
AAACAGTCCC TGTCCTTTAG AGTTATTGAT CAACAACAAA TAGAACGTCA CTTTACAATG 
AATGAACTGA CTGAACTTTA CACCTTTGAG CCTGATCTGC TGGATGACCC TAATTCCGAA 
AAGAAGAAAA AGCGAGAGAC TCCCATGCTG CCTAAGGATA CTGTGCTAGC AGAGCTCTTA 
CAAATCTTGA AGGAGTACAT AGTCACTTAT CATGAGCACG ACTCTCTTCT GGATCATAAG 
GAGGAAGAGG CCCTTACAGA AGAGGATCGG AAGGCAGCAT GGGCAGAATA TGAAGCTGAA 
AAGAAGGGTT TGACCAATCG TTTTAACGTG GCAGCAGTAA CTGGGGCCAA CTACGCTCAA 
GTCAATATAA ACTCGCAATC TTCATACATG CCTTTAAACT TGGGAGCGCT GTCTTCTTTA 
TCCAATCAGC AACTCGAGGA TTTAATAAAT CAAGGCAGAG AAAAAGTGGT TGAAGCTACA 
AATAACATAG CAACTGCAAG GATTCAACCC CTTGAAGACA TTATCTCGAC AGTGTGGAAG 
GAAAATCCGA CTGCCACGGA GGCCCAAGTT CAGTCCCTTG CCCTAAGCAG GCAGTCGAGC 
CAAGAATTGG AAATAAAGAG ACGTGAGGCC ATTTATAACG ACGTACTGAC TAAGCAACAG 
ATGTTGATCA GCTGCGTGCA GCGAATTCTC ATGAGTCGAA GGTTACAGCA CCAATACAAC 
CAGCAACAGC AGCAGCAACA AATTGTTTTT CAACAGCAAG CAGCTCTGAA TCGCCTGATG 
ATGCAGAAAA ATCCAAATAT TATAATATCC CCTTCTAACT TTTCTCAAAT TGACATGAGA 
GGAATGTACC AAGCTGGGAT GCAAGGTCAT CAGATGCAGC GTAATGCACC CGCCTCTATA 
AGAGCAAAAA ATCCAGGACC TTCCCAGGGA AAAAAAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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atrx cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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