greb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	greb1
Entrez Gene ID	100379724
Full Name	growth regulation by estrogen in breast cancer 1
General protein information	Preferred Names growth regulation by estrogen in breast cancer 1 Names protein GREB1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012963459.2	XP_012818913.1	protein GREB1 isoform X2
XM_012963456.3	XP_012818910.1	protein GREB1 isoform X1
XM_018094481.1	XP_017949970.1	protein GREB1 isoform X3
XM_012963456.2	XP_012818910.1	protein GREB1 isoform X1
XM_012963457.2	XP_012818911.1	protein GREB1 isoform X1
XM_012963457.3	XP_012818911.1	protein GREB1 isoform X1
XM_012963459.3	XP_012818913.1	protein GREB1 isoform X2

Pathways from BioSystems

Homologies

	GREB1	XP_001089374.1
Pan troglodytes (chimpanzee)	GREB1	XP_515304.3
Bos taurus (cattle)	GREB1	NP_001192560.1
Danio rerio (zebrafish)	greb1	XP_005158874.1
Homo sapiens (human)	GREB1	NP_055483.2
Canis lupus familiaris (dog)	GREB1	XP_005630186.1
Mus musculus (house mouse)	Greb1	NP_056579.2
Rattus norvegicus (Norway rat)	Greb1	XP_006239984.1
Gallus gallus (chicken)	GREB1	XP_004935825.1
Xenopus tropicalis (tropical clawed frog)	greb1	XP_002942230.2

Gene Ontology

The following greb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the greb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32883	XM_012963456.3 Latest version!	Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa32883	XM_012963457.3 Latest version!	Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa32884	XM_012963459.3 Latest version!	Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa44820	XM_018094481.1 Latest version!	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
OXa32883	XM_012963456.2	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32883	XM_012963457.2	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32884	XM_012963459.2	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

greb1 ( XM_012963456.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818910.1 Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32883
Clone ID Related Accession (Same CDS sequence)	XM_012963456.3 , XM_012963456.2 , XM_012963457.2 , XM_012963457.3
Accession Version	XM_012963456.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963456.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818910.1
CDS	120..5816
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTEVRSQNLSANMCSSTVPAMQDGPGSMNKSSDPPTAPNSYLNSGGVLTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPDGDLDFLLDKFYQENQGAATLSPIKPSTETPRPICTSYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_012963456.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963456.3

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

greb1 ( XM_012963457.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818911.1 Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X2, mRNA.

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CloneID	OXa32883
Clone ID Related Accession (Same CDS sequence)	XM_012963456.3 , XM_012963456.2 , XM_012963457.2 , XM_012963457.3
Accession Version	XM_012963457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818911.1
CDS	115..5811
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTEVRSQNLSANMCSSTVPAMQDGPGSMNKSSDPPTAPNSYLNSGGVLTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPDGDLDFLLDKFYQENQGAATLSPIKPSTETPRPICTSYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_012963457.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963457.3

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

greb1 ( XM_012963459.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818913.1 Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X3, mRNA.

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CloneID	OXa32884
Clone ID Related Accession (Same CDS sequence)	XM_012963459.2 , XM_012963459.3
Accession Version	XM_012963459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGG TTACAACACT GAGTGGCACC ATGTCCGCCC GTCCCAGCTG 
GCCGTGGCAC AAAAGCTCTT ATCACATGTC TGCTCCATCG CCGACTCCAG CACTCAGAAC 
CTTGATTTGG GCTCATTTGA GAAAGTGGAT TTTCTTATCT GTGTTCCTCC ATCGGAGATA 
ACCTACCAGC AAACACTCTC TCATCTGTGG AGTTCAGGGG TGCTACTGGA GCTTGGTGTG 
GAGAAGGCCT TGCTCACAAA GCAGCTGCTG GAGCAGTACA TAATGAAACT GGATTCCGAC 
GCGCCCAATA AATTCAAGAC GTTCCTCCAC AGAGCCCTGC AGAACCCTCA CACCTTGTTT 
GTGCTCGTTC ATGACCACGC ACATTGGGAT CTCACCAGTT CTGTTTATAG CCCTTATGCT 
CAAAGTGAGT CCAACATGGG CCTTGTTGAT CGACTGCTAA ACTGCAGAGA AGTGAGAGAC 
GCGCCCAATA TCTTGGTTTT ACACGTGACT TCCTTTCCTT ATGCTCTGCA AACACAGAAC 
ACCCGGATCA GCCCATACAA TGAAATACAC TGGCCTGCTT CACACAGTCA TGGGATGGAA 
TTGTACCACG AGAGCAGGAA ATACTTCGGT CTGTCCGAGT TTATTGAATC AACCGTTTCT 
GGCCACAGCC TTCCTTTGCT CCGCCATGAC AGCTCCTTTG AAGCCATCGT ACTGGCACTA 
GGAAAGAGGT TCCCCAAGCT CCACAGTGCC GTTATCCGGA CGTTCCTTCT CATCCAGCAT 
TACTCTGCTG CTATGATGTC TGTCTGTGGT TTATCGCAAA TGAAGAATTA CACGTCTGTG 
GAAACACTGG AGATCATTCA GAACCTCCTG AGCTCCCCTC AGCAGTGTGC CAGTGCCCAC 
GGCCTCATGG TCCTTTTAAG GATCCCATGC ATCCCATTGG CCACTCTTGC CTACGAGCGC 
CTGTGTCACG TTCGAGAACG CCTCGGGCTG CAGCATCGAT TTGAAATTAT AATGGCTACA 
TCGAACTCAA CCATAAGTAT TAGCAAGCAC TTTGTGGACC AGCTACGGGC CTGGCAGAAG 
AATGAAGATG CAAACTGGCA GCCACATACA TATCTGGAAC TGGAAGGTCT GCCCTGTATC 
CTGATTTCTA CTGGAATTGA TCCCCTAGGA GAGTCTTTGC CAAGGTCATT GAGATACTGT 
GACCTCAGGT TAGTACATTT CTGCACCTGG GAAAGGACCA AGATGGAGCA AGAGCTGGGA 
TTAGCGTCAA GCTTCATTAA CACAGCTGTA TACACTGACA AAAGTTTGGG CAGGGACACG 
TTTGAGAGTG ACCCGGAGAA ACTCAGCAGC ACGGACAATG AAGATGAGGA AATGGTGATC 
GATGGCTCAA GGACCCCCTG TAAAATACAA AGCTCTCGCT CGCACAACTC AACGTCATCT 
GTGTGTTCCA AAGCTTCAGA GGAGACACTA AGGCCACCAT TGCAAGTGAG TGCTGCTAAT 
GTGGAGAAGA TGAAACACAC AGTGGATAAA GGCACCGGGA ATCCCATCTC TTGTGAACAA 
TCAGAGAGAA CTAACAGTCC TGATCCAGCT GTGAAGAACA CTCCAAGTTC AACATCTGCA 
CCGTCCTCTT CCTCACCTTC CTGTTTCCCC AATGGGATCG ACCTGCAGAC TTCACAATGT 
TCTATGTCCA AGGCATATAA GCAGCCGCCA ATAGTTTTTC TCCCCAAACT GGTTTATGAC 
ATTATTACCT CCACTGACAG CAGTGGCCTT TCTAAATCTT CATCTTTCCT GCCACATTGT 
GAAGTCTCAT GGGCCAGCTC TTTCCGCCCC CTTCTGAGTA AAATGATGAC TAGTACAGAG 
CAGTCTTTGT ACTACCGCCA ATGGACGGTG CCCAAACAGA GCCACATGGA CTACTGCAAT 
ATTAATGAAG GCAGAATGGA TACCTTCCAC CCCAGGAGGC TGCTACTCAG TGGACCACCT 
CAGATAGGCA AAACTGGAGC TTATCTGCAG TTCCTCAGCG TCTTGTCCAG AATGCTGATT 
AGACTGACAG AGGTGGATGT ATATGATGAA GAAGAAATCA ATATCAGCCC CAGAGACAAA 
TCCGACCAGC ATTTCCACCA GATTTTGGAT ACGTGGCCTA ATGTGGAAAC TATAAAGGAG 
ATGGCTTTTG ATTACACTTT ACATGACCCC AAGTATGAAG ATGCCAGTGC CATTTGCTCA 
GCTTTTCAGG ATTTCCACAC TGAAGGTAAA CCAGTCCGAA AGAAGAATGA AGATGTTTAT 
ATGAGGCGCC CCACGACCAG AATGAGGTTA TCAAAGTATG CCTCCTACAA CACGTACCAC 
CATTGTGAGC AGTGCCACCA ATACATGGGA TACAACCCCA GGTTCCAGAT GTACGAATCC 
ACCCTGCATG CTTTTGCATT CTCCTATTCA CTGTTGGGTG AAGAAATTCA GCTGCACTTC 
ATCATACCCA AGTCCAAAGA ACATCACTTT GTCTTTAGCC AGCCGGGGGG CCAGCTGGAA 
AGCATGAGGT TGCCATTGGT CGCAGATAAG AATCCTGACT GTGTGAAGAG TCCCATCTTT 
ACTCCAACCA CAGGCCGCCA TGAACACGGG CTCTTCAATC TCTATCATGC TATGGATGGC 
AGCAGCCATT TGCACATTTT GGTGGTCAAG GAATATGAGA TGGCGATATA TAAAAAATAC 
TGGCCAAACC ACATCATGTT GGTTCTCCCC AGTATCTTTA ACAGCGCCGG TGTAGGGGCA 
GCTCATTTCC TAATCAAAGA GCTTTCTTAT CATAACCTGG AGCTGGAGCG CAACAGACAA 
GGGGAGCTGG GCATCAGGCC ACAAGATGTC TGGCCTTTCG TCGTCATTGC TGACGATTCG 
TGTGTCATGT GGAACACTGT GGATGTCAGC GTAACGGGGG ATCAACCTAG GGATTCTTCA 
TGTTCAGAAA GAAACGTATC TTTGAAGTAC ATCCTTCAGC ACATGGAAGC CTCCCAGGAC 
ATTACCCAGT ATGCTCTGCT TGGAATGCGG AAGTGGACGA GTAAGAACAA TGAAAGGGAT 
ATGAAGGAGC CTTTCTCCCG CTGCCATGTC CATGATTTTG TCCTGCTCAA CGTAAACCTG 
ACGCAGGATG TACAGTACAA CCAAAACAGA TTTACCTGCG ATGATGTTGA TTTCAATCTT 
CGGGTGAACA GCTCTGGACT GTTAATAGGC CGATTCAACC GCTTCAGTGT TATGAAAAAG 
CAGATTGCAG TTGGTGGCCA TAGATCCCTA CATATCAAAT CAAAGGTTCT AGACCCTTCA 
GTGTCCGTTT CCCCTGCTCA GTACATCTGT GCCCCAGACA GCAAGCACAC AATTCTAGCT 
GCACCGGCTC ATCTGCTGCT CGAGAAATTC CTTCAGTTTC ACAGCCACAG ATTCTTTCCA 
ATATCACTCA ACAATAGCAG CCACCCTGTG CTTATAGTGG ATTGCTACCT TAACCTGGGG 
CCACAGATAA GAGTTTGCTA TGTGAGCTCT CGCCCTCACT CTACCAACAT TAGCTGCCCC 
GGAGTGCTCT TCAGCGGCCT CCTCCTGCAC CTTTCTGACT CCTTTGTGGT TGCTGGGTTT 
TTGAAAAGGT TTCAGTTTCT GAAAGGGGCG ACGTTGTGCG TGACATGTCA GGACCGCAAC 
TCTCTGCGCC AGACCGTCGT TCGGCTGGAA CTGGAAGAAG AATGGCAGTT CCGGCTACGA 
GATGAATTCC AAACCGCTAA TGCTAAAGAA GACAAGCCTC TGTTCATCCT TACTGGCCGC 
CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818913.1
CDS	121..5709
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTEVRSQNLSANMCSSTVPAMQDGPGSMNKSSDPPTAPNSYLNSGGVLTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPGYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_012963459.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulating estrogen receptor binding 1 (greb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963459.3

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGG TTACAACACT GAGTGGCACC ATGTCCGCCC GTCCCAGCTG 
GCCGTGGCAC AAAAGCTCTT ATCACATGTC TGCTCCATCG CCGACTCCAG CACTCAGAAC 
CTTGATTTGG GCTCATTTGA GAAAGTGGAT TTTCTTATCT GTGTTCCTCC ATCGGAGATA 
ACCTACCAGC AAACACTCTC TCATCTGTGG AGTTCAGGGG TGCTACTGGA GCTTGGTGTG 
GAGAAGGCCT TGCTCACAAA GCAGCTGCTG GAGCAGTACA TAATGAAACT GGATTCCGAC 
GCGCCCAATA AATTCAAGAC GTTCCTCCAC AGAGCCCTGC AGAACCCTCA CACCTTGTTT 
GTGCTCGTTC ATGACCACGC ACATTGGGAT CTCACCAGTT CTGTTTATAG CCCTTATGCT 
CAAAGTGAGT CCAACATGGG CCTTGTTGAT CGACTGCTAA ACTGCAGAGA AGTGAGAGAC 
GCGCCCAATA TCTTGGTTTT ACACGTGACT TCCTTTCCTT ATGCTCTGCA AACACAGAAC 
ACCCGGATCA GCCCATACAA TGAAATACAC TGGCCTGCTT CACACAGTCA TGGGATGGAA 
TTGTACCACG AGAGCAGGAA ATACTTCGGT CTGTCCGAGT TTATTGAATC AACCGTTTCT 
GGCCACAGCC TTCCTTTGCT CCGCCATGAC AGCTCCTTTG AAGCCATCGT ACTGGCACTA 
GGAAAGAGGT TCCCCAAGCT CCACAGTGCC GTTATCCGGA CGTTCCTTCT CATCCAGCAT 
TACTCTGCTG CTATGATGTC TGTCTGTGGT TTATCGCAAA TGAAGAATTA CACGTCTGTG 
GAAACACTGG AGATCATTCA GAACCTCCTG AGCTCCCCTC AGCAGTGTGC CAGTGCCCAC 
GGCCTCATGG TCCTTTTAAG GATCCCATGC ATCCCATTGG CCACTCTTGC CTACGAGCGC 
CTGTGTCACG TTCGAGAACG CCTCGGGCTG CAGCATCGAT TTGAAATTAT AATGGCTACA 
TCGAACTCAA CCATAAGTAT TAGCAAGCAC TTTGTGGACC AGCTACGGGC CTGGCAGAAG 
AATGAAGATG CAAACTGGCA GCCACATACA TATCTGGAAC TGGAAGGTCT GCCCTGTATC 
CTGATTTCTA CTGGAATTGA TCCCCTAGGA GAGTCTTTGC CAAGGTCATT GAGATACTGT 
GACCTCAGGT TAGTACATTT CTGCACCTGG GAAAGGACCA AGATGGAGCA AGAGCTGGGA 
TTAGCGTCAA GCTTCATTAA CACAGCTGTA TACACTGACA AAAGTTTGGG CAGGGACACG 
TTTGAGAGTG ACCCGGAGAA ACTCAGCAGC ACGGACAATG AAGATGAGGA AATGGTGATC 
GATGGCTCAA GGACCCCCTG TAAAATACAA AGCTCTCGCT CGCACAACTC AACGTCATCT 
GTGTGTTCCA AAGCTTCAGA GGAGACACTA AGGCCACCAT TGCAAGTGAG TGCTGCTAAT 
GTGGAGAAGA TGAAACACAC AGTGGATAAA GGCACCGGGA ATCCCATCTC TTGTGAACAA 
TCAGAGAGAA CTAACAGTCC TGATCCAGCT GTGAAGAACA CTCCAAGTTC AACATCTGCA 
CCGTCCTCTT CCTCACCTTC CTGTTTCCCC AATGGGATCG ACCTGCAGAC TTCACAATGT 
TCTATGTCCA AGGCATATAA GCAGCCGCCA ATAGTTTTTC TCCCCAAACT GGTTTATGAC 
ATTATTACCT CCACTGACAG CAGTGGCCTT TCTAAATCTT CATCTTTCCT GCCACATTGT 
GAAGTCTCAT GGGCCAGCTC TTTCCGCCCC CTTCTGAGTA AAATGATGAC TAGTACAGAG 
CAGTCTTTGT ACTACCGCCA ATGGACGGTG CCCAAACAGA GCCACATGGA CTACTGCAAT 
ATTAATGAAG GCAGAATGGA TACCTTCCAC CCCAGGAGGC TGCTACTCAG TGGACCACCT 
CAGATAGGCA AAACTGGAGC TTATCTGCAG TTCCTCAGCG TCTTGTCCAG AATGCTGATT 
AGACTGACAG AGGTGGATGT ATATGATGAA GAAGAAATCA ATATCAGCCC CAGAGACAAA 
TCCGACCAGC ATTTCCACCA GATTTTGGAT ACGTGGCCTA ATGTGGAAAC TATAAAGGAG 
ATGGCTTTTG ATTACACTTT ACATGACCCC AAGTATGAAG ATGCCAGTGC CATTTGCTCA 
GCTTTTCAGG ATTTCCACAC TGAAGGTAAA CCAGTCCGAA AGAAGAATGA AGATGTTTAT 
ATGAGGCGCC CCACGACCAG AATGAGGTTA TCAAAGTATG CCTCCTACAA CACGTACCAC 
CATTGTGAGC AGTGCCACCA ATACATGGGA TACAACCCCA GGTTCCAGAT GTACGAATCC 
ACCCTGCATG CTTTTGCATT CTCCTATTCA CTGTTGGGTG AAGAAATTCA GCTGCACTTC 
ATCATACCCA AGTCCAAAGA ACATCACTTT GTCTTTAGCC AGCCGGGGGG CCAGCTGGAA 
AGCATGAGGT TGCCATTGGT CGCAGATAAG AATCCTGACT GTGTGAAGAG TCCCATCTTT 
ACTCCAACCA CAGGCCGCCA TGAACACGGG CTCTTCAATC TCTATCATGC TATGGATGGC 
AGCAGCCATT TGCACATTTT GGTGGTCAAG GAATATGAGA TGGCGATATA TAAAAAATAC 
TGGCCAAACC ACATCATGTT GGTTCTCCCC AGTATCTTTA ACAGCGCCGG TGTAGGGGCA 
GCTCATTTCC TAATCAAAGA GCTTTCTTAT CATAACCTGG AGCTGGAGCG CAACAGACAA 
GGGGAGCTGG GCATCAGGCC ACAAGATGTC TGGCCTTTCG TCGTCATTGC TGACGATTCG 
TGTGTCATGT GGAACACTGT GGATGTCAGC GTAACGGGGG ATCAACCTAG GGATTCTTCA 
TGTTCAGAAA GAAACGTATC TTTGAAGTAC ATCCTTCAGC ACATGGAAGC CTCCCAGGAC 
ATTACCCAGT ATGCTCTGCT TGGAATGCGG AAGTGGACGA GTAAGAACAA TGAAAGGGAT 
ATGAAGGAGC CTTTCTCCCG CTGCCATGTC CATGATTTTG TCCTGCTCAA CGTAAACCTG 
ACGCAGGATG TACAGTACAA CCAAAACAGA TTTACCTGCG ATGATGTTGA TTTCAATCTT 
CGGGTGAACA GCTCTGGACT GTTAATAGGC CGATTCAACC GCTTCAGTGT TATGAAAAAG 
CAGATTGCAG TTGGTGGCCA TAGATCCCTA CATATCAAAT CAAAGGTTCT AGACCCTTCA 
GTGTCCGTTT CCCCTGCTCA GTACATCTGT GCCCCAGACA GCAAGCACAC AATTCTAGCT 
GCACCGGCTC ATCTGCTGCT CGAGAAATTC CTTCAGTTTC ACAGCCACAG ATTCTTTCCA 
ATATCACTCA ACAATAGCAG CCACCCTGTG CTTATAGTGG ATTGCTACCT TAACCTGGGG 
CCACAGATAA GAGTTTGCTA TGTGAGCTCT CGCCCTCACT CTACCAACAT TAGCTGCCCC 
GGAGTGCTCT TCAGCGGCCT CCTCCTGCAC CTTTCTGACT CCTTTGTGGT TGCTGGGTTT 
TTGAAAAGGT TTCAGTTTCT GAAAGGGGCG ACGTTGTGCG TGACATGTCA GGACCGCAAC 
TCTCTGCGCC AGACCGTCGT TCGGCTGGAA CTGGAAGAAG AATGGCAGTT CCGGCTACGA 
GATGAATTCC AAACCGCTAA TGCTAAAGAA GACAAGCCTC TGTTCATCCT TACTGGCCGC 
CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

greb1 ( XM_018094481.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949970.1 Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X4, mRNA.

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CloneID	OXa44820
Clone ID Related Accession (Same CDS sequence)	XM_018094481.1
Accession Version	XM_018094481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGGAGATA ATGGCTACTT ACAAGTCAGC 
TCCTCATACC AGAATATGAA TGGGTACCTG TGCCCCCAGC AGTCTGCAAA GGTGAATCCC 
TCAGCACCAG CAAGGGCCTC GGTTTTAGGA AATTTGACAA ATTCTGCCCC CCCAAAAAAA 
CGCCACAAAG GATGGTCTCC AGAGTCGCCA ACATCAACAG ATGCCAGCTT TGCACAGAGC 
AATTTTATTA ACTCAAGTAT CTGTGGAAAA GGTGATGCCA TGCCTCAGAT GTGTGCCACA 
CAATCCACCC CAGGAGGCCT TCTGTCTTCA TCCATGGTGG TGAGAGGAGA GTCTGTGTCT 
GTACCCGAGA ACCTGCTGAA AACCAGCATG ATTAAGCCTG TCATTTTCAA GGGCCACGGG 
AGCTTCCCCT ACCTCTGTGG AAATGTGAAT GATGTGATTG TGAGCCCTCT CCTGCACAAC 
TGCTACACAA ACTCTCAGTC TATGCCCAAA GCCTATGAGC AGTACGGCCC CACCACAATT 
CAGCCCATCT CAGAGGAAAT GCAGCTCCTG CTGACAGTCT ATTATCTGGT CCAGCTTGCT 
CCAGACCAGG TTCCCTTGAT AGAGGACTTA GAGCAGATAT TCATGCGTTC ATGGAGGGAA 
ACTCACCTGG CTGAAATCCG CCAGTATCAG CAGACCGTGT CGCAGGTTTT CCCGCACCTT 
CCCAGCCCCA TAACTCCTGT GACTGCTGCG CAGCTTCCCT GGCTCGCCGG CCTCGCCGCC 
AGCTCCTGTA ACGAGAGTGT GAAGATCATT GAGTGCAGCT ATTCCTTAGC GGAGGGGCTC 
TGTGAGATGT TCAAGCTGCT GGTGGAAGGG AAACTGTCAA AGACCAATTA TGTGGTAATC 
ATCTGCCCTT CCAGAAGCAA TGGTATAGAT TCCTGCATTG TAATAACAGG AAAATACCAG 
GCCCGGATTT TGGTAGAAAG CATGTTCTCT CCATCAGATT ACCTAAAAGA GGTGACGTAT 
GAATTAGCCA GCGGGAAGAT GGAGAATTTA GCAGCTTTTT TTCATGCATT GTGCCCAGAT 
GGTGATTTAG ATTTCCTGCT GGACAAGTTT TATCAGGAAA ATCAAGGCGC TGCCACGTTA 
TCACCAATTA AGCCAAGCAC GGAAACACCA CGTCCTATAT GCACTAGTTA CAACACTGAG 
TGGCACCATG TCCGCCCGTC CCAGCTGGCC GTGGCACAAA AGCTCTTATC ACATGTCTGC 
TCCATCGCCG ACTCCAGCAC TCAGAACCTT GATTTGGGCT CATTTGAGAA AGTGGATTTT 
CTTATCTGTG TTCCTCCATC GGAGATAACC TACCAGCAAA CACTCTCTCA TCTGTGGAGT 
TCAGGGGTGC TACTGGAGCT TGGTGTGGAG AAGGCCTTGC TCACAAAGCA GCTGCTGGAG 
CAGTACATAA TGAAACTGGA TTCCGACGCG CCCAATAAAT TCAAGACGTT CCTCCACAGA 
GCCCTGCAGA ACCCTCACAC CTTGTTTGTG CTCGTTCATG ACCACGCACA TTGGGATCTC 
ACCAGTTCTG TTTATAGCCC TTATGCTCAA AGTGAGTCCA ACATGGGCCT TGTTGATCGA 
CTGCTAAACT GCAGAGAAGT GAGAGACGCG CCCAATATCT TGGTTTTACA CGTGACTTCC 
TTTCCTTATG CTCTGCAAAC ACAGAACACC CGGATCAGCC CATACAATGA AATACACTGG 
CCTGCTTCAC ACAGTCATGG GATGGAATTG TACCACGAGA GCAGGAAATA CTTCGGTCTG 
TCCGAGTTTA TTGAATCAAC CGTTTCTGGC CACAGCCTTC CTTTGCTCCG CCATGACAGC 
TCCTTTGAAG CCATCGTACT GGCACTAGGA AAGAGGTTCC CCAAGCTCCA CAGTGCCGTT 
ATCCGGACGT TCCTTCTCAT CCAGCATTAC TCTGCTGCTA TGATGTCTGT CTGTGGTTTA 
TCGCAAATGA AGAATTACAC GTCTGTGGAA ACACTGGAGA TCATTCAGAA CCTCCTGAGC 
TCCCCTCAGC AGTGTGCCAG TGCCCACGGC CTCATGGTCC TTTTAAGGAT CCCATGCATC 
CCATTGGCCA CTCTTGCCTA CGAGCGCCTG TGTCACGTTC GAGAACGCCT CGGGCTGCAG 
CATCGATTTG AAATTATAAT GGCTACATCG AACTCAACCA TAAGTATTAG CAAGCACTTT 
GTGGACCAGC TACGGGCCTG GCAGAAGAAT GAAGATGCAA ACTGGCAGCC ACATACATAT 
CTGGAACTGG AAGGTCTGCC CTGTATCCTG ATTTCTACTG GAATTGATCC CCTAGGAGAG 
TCTTTGCCAA GGTCATTGAG ATACTGTGAC CTCAGGTTAG TACATTTCTG CACCTGGGAA 
AGGACCAAGA TGGAGCAAGA GCTGGGATTA GCGTCAAGCT TCATTAACAC AGCTGTATAC 
ACTGACAAAA GTTTGGGCAG GGACACGTTT GAGAGTGACC CGGAGAAACT CAGCAGCACG 
GACAATGAAG ATGAGGAAAT GGTGATCGAT GGCTCAAGGA CCCCCTGTAA AATACAAAGC 
TCTCGCTCGC ACAACTCAAC GTCATCTGTG TGTTCCAAAG CTTCAGAGGA GACACTAAGG 
CCACCATTGC AAGTGAGTGC TGCTAATGTG GAGAAGATGA AACACACAGT GGATAAAGGC 
ACCGGGAATC CCATCTCTTG TGAACAATCA GAGAGAACTA ACAGTCCTGA TCCAGCTGTG 
AAGAACACTC CAAGTTCAAC ATCTGCACCG TCCTCTTCCT CACCTTCCTG TTTCCCCAAT 
GGGATCGACC TGCAGACTTC ACAATGTTCT ATGTCCAAGG CATATAAGCA GCCGCCAATA 
GTTTTTCTCC CCAAACTGGT TTATGACATT ATTACCTCCA CTGACAGCAG TGGCCTTTCT 
AAATCTTCAT CTTTCCTGCC ACATTGTGAA GTCTCATGGG CCAGCTCTTT CCGCCCCCTT 
CTGAGTAAAA TGATGACTAG TACAGAGCAG TCTTTGTACT ACCGCCAATG GACGGTGCCC 
AAACAGAGCC ACATGGACTA CTGCAATATT AATGAAGGCA GAATGGATAC CTTCCACCCC 
AGGAGGCTGC TACTCAGTGG ACCACCTCAG ATAGGCAAAA CTGGAGCTTA TCTGCAGTTC 
CTCAGCGTCT TGTCCAGAAT GCTGATTAGA CTGACAGAGG TGGATGTATA TGATGAAGAA 
GAAATCAATA TCAGCCCCAG AGACAAATCC GACCAGCATT TCCACCAGAT TTTGGATACG 
TGGCCTAATG TGGAAACTAT AAAGGAGATG GCTTTTGATT ACACTTTACA TGACCCCAAG 
TATGAAGATG CCAGTGCCAT TTGCTCAGCT TTTCAGGATT TCCACACTGA AGGTAAACCA 
GTCCGAAAGA AGAATGAAGA TGTTTATATG AGGCGCCCCA CGACCAGAAT GAGGTTATCA 
AAGTATGCCT CCTACAACAC GTACCACCAT TGTGAGCAGT GCCACCAATA CATGGGATAC 
AACCCCAGGT TCCAGATGTA CGAATCCACC CTGCATGCTT TTGCATTCTC CTATTCACTG 
TTGGGTGAAG AAATTCAGCT GCACTTCATC ATACCCAAGT CCAAAGAACA TCACTTTGTC 
TTTAGCCAGC CGGGGGGCCA GCTGGAAAGC ATGAGGTTGC CATTGGTCGC AGATAAGAAT 
CCTGACTGTG TGAAGAGTCC CATCTTTACT CCAACCACAG GCCGCCATGA ACACGGGCTC 
TTCAATCTCT ATCATGCTAT GGATGGCAGC AGCCATTTGC ACATTTTGGT GGTCAAGGAA 
TATGAGATGG CGATATATAA AAAATACTGG CCAAACCACA TCATGTTGGT TCTCCCCAGT 
ATCTTTAACA GCGCCGGTGT AGGGGCAGCT CATTTCCTAA TCAAAGAGCT TTCTTATCAT 
AACCTGGAGC TGGAGCGCAA CAGACAAGGG GAGCTGGGCA TCAGGCCACA AGATGTCTGG 
CCTTTCGTCG TCATTGCTGA CGATTCGTGT GTCATGTGGA ACACTGTGGA TGTCAGCGTA 
ACGGGGGATC AACCTAGGGA TTCTTCATGT TCAGAAAGAA ACGTATCTTT GAAGTACATC 
CTTCAGCACA TGGAAGCCTC CCAGGACATT ACCCAGTATG CTCTGCTTGG AATGCGGAAG 
TGGACGAGTA AGAACAATGA AAGGGATATG AAGGAGCCTT TCTCCCGCTG CCATGTCCAT 
GATTTTGTCC TGCTCAACGT AAACCTGACG CAGGATGTAC AGTACAACCA AAACAGATTT 
ACCTGCGATG ATGTTGATTT CAATCTTCGG GTGAACAGCT CTGGACTGTT AATAGGCCGA 
TTCAACCGCT TCAGTGTTAT GAAAAAGCAG ATTGCAGTTG GTGGCCATAG ATCCCTACAT 
ATCAAATCAA AGGTTCTAGA CCCTTCAGTG TCCGTTTCCC CTGCTCAGTA CATCTGTGCC 
CCAGACAGCA AGCACACAAT TCTAGCTGCA CCGGCTCATC TGCTGCTCGA GAAATTCCTT 
CAGTTTCACA GCCACAGATT CTTTCCAATA TCACTCAACA ATAGCAGCCA CCCTGTGCTT 
ATAGTGGATT GCTACCTTAA CCTGGGGCCA CAGATAAGAG TTTGCTATGT GAGCTCTCGC 
CCTCACTCTA CCAACATTAG CTGCCCCGGA GTGCTCTTCA GCGGCCTCCT CCTGCACCTT 
TCTGACTCCT TTGTGGTTGC TGGGTTTTTG AAAAGGTTTC AGTTTCTGAA AGGGGCGACG 
TTGTGCGTGA CATGTCAGGA CCGCAACTCT CTGCGCCAGA CCGTCGTTCG GCTGGAACTG 
GAAGAAGAAT GGCAGTTCCG GCTACGAGAT GAATTCCAAA CCGCTAATGC TAAAGAAGAC 
AAGCCTCTGT TCATCCTTAC TGGCCGCCAT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949970.1
CDS	139..5694
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPDGDLDFLLDKFYQENQGAATLSPIKPSTETPRPICTSYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_018094481.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094481.1

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGGAGATA ATGGCTACTT ACAAGTCAGC 
TCCTCATACC AGAATATGAA TGGGTACCTG TGCCCCCAGC AGTCTGCAAA GGTGAATCCC 
TCAGCACCAG CAAGGGCCTC GGTTTTAGGA AATTTGACAA ATTCTGCCCC CCCAAAAAAA 
CGCCACAAAG GATGGTCTCC AGAGTCGCCA ACATCAACAG ATGCCAGCTT TGCACAGAGC 
AATTTTATTA ACTCAAGTAT CTGTGGAAAA GGTGATGCCA TGCCTCAGAT GTGTGCCACA 
CAATCCACCC CAGGAGGCCT TCTGTCTTCA TCCATGGTGG TGAGAGGAGA GTCTGTGTCT 
GTACCCGAGA ACCTGCTGAA AACCAGCATG ATTAAGCCTG TCATTTTCAA GGGCCACGGG 
AGCTTCCCCT ACCTCTGTGG AAATGTGAAT GATGTGATTG TGAGCCCTCT CCTGCACAAC 
TGCTACACAA ACTCTCAGTC TATGCCCAAA GCCTATGAGC AGTACGGCCC CACCACAATT 
CAGCCCATCT CAGAGGAAAT GCAGCTCCTG CTGACAGTCT ATTATCTGGT CCAGCTTGCT 
CCAGACCAGG TTCCCTTGAT AGAGGACTTA GAGCAGATAT TCATGCGTTC ATGGAGGGAA 
ACTCACCTGG CTGAAATCCG CCAGTATCAG CAGACCGTGT CGCAGGTTTT CCCGCACCTT 
CCCAGCCCCA TAACTCCTGT GACTGCTGCG CAGCTTCCCT GGCTCGCCGG CCTCGCCGCC 
AGCTCCTGTA ACGAGAGTGT GAAGATCATT GAGTGCAGCT ATTCCTTAGC GGAGGGGCTC 
TGTGAGATGT TCAAGCTGCT GGTGGAAGGG AAACTGTCAA AGACCAATTA TGTGGTAATC 
ATCTGCCCTT CCAGAAGCAA TGGTATAGAT TCCTGCATTG TAATAACAGG AAAATACCAG 
GCCCGGATTT TGGTAGAAAG CATGTTCTCT CCATCAGATT ACCTAAAAGA GGTGACGTAT 
GAATTAGCCA GCGGGAAGAT GGAGAATTTA GCAGCTTTTT TTCATGCATT GTGCCCAGAT 
GGTGATTTAG ATTTCCTGCT GGACAAGTTT TATCAGGAAA ATCAAGGCGC TGCCACGTTA 
TCACCAATTA AGCCAAGCAC GGAAACACCA CGTCCTATAT GCACTAGTTA CAACACTGAG 
TGGCACCATG TCCGCCCGTC CCAGCTGGCC GTGGCACAAA AGCTCTTATC ACATGTCTGC 
TCCATCGCCG ACTCCAGCAC TCAGAACCTT GATTTGGGCT CATTTGAGAA AGTGGATTTT 
CTTATCTGTG TTCCTCCATC GGAGATAACC TACCAGCAAA CACTCTCTCA TCTGTGGAGT 
TCAGGGGTGC TACTGGAGCT TGGTGTGGAG AAGGCCTTGC TCACAAAGCA GCTGCTGGAG 
CAGTACATAA TGAAACTGGA TTCCGACGCG CCCAATAAAT TCAAGACGTT CCTCCACAGA 
GCCCTGCAGA ACCCTCACAC CTTGTTTGTG CTCGTTCATG ACCACGCACA TTGGGATCTC 
ACCAGTTCTG TTTATAGCCC TTATGCTCAA AGTGAGTCCA ACATGGGCCT TGTTGATCGA 
CTGCTAAACT GCAGAGAAGT GAGAGACGCG CCCAATATCT TGGTTTTACA CGTGACTTCC 
TTTCCTTATG CTCTGCAAAC ACAGAACACC CGGATCAGCC CATACAATGA AATACACTGG 
CCTGCTTCAC ACAGTCATGG GATGGAATTG TACCACGAGA GCAGGAAATA CTTCGGTCTG 
TCCGAGTTTA TTGAATCAAC CGTTTCTGGC CACAGCCTTC CTTTGCTCCG CCATGACAGC 
TCCTTTGAAG CCATCGTACT GGCACTAGGA AAGAGGTTCC CCAAGCTCCA CAGTGCCGTT 
ATCCGGACGT TCCTTCTCAT CCAGCATTAC TCTGCTGCTA TGATGTCTGT CTGTGGTTTA 
TCGCAAATGA AGAATTACAC GTCTGTGGAA ACACTGGAGA TCATTCAGAA CCTCCTGAGC 
TCCCCTCAGC AGTGTGCCAG TGCCCACGGC CTCATGGTCC TTTTAAGGAT CCCATGCATC 
CCATTGGCCA CTCTTGCCTA CGAGCGCCTG TGTCACGTTC GAGAACGCCT CGGGCTGCAG 
CATCGATTTG AAATTATAAT GGCTACATCG AACTCAACCA TAAGTATTAG CAAGCACTTT 
GTGGACCAGC TACGGGCCTG GCAGAAGAAT GAAGATGCAA ACTGGCAGCC ACATACATAT 
CTGGAACTGG AAGGTCTGCC CTGTATCCTG ATTTCTACTG GAATTGATCC CCTAGGAGAG 
TCTTTGCCAA GGTCATTGAG ATACTGTGAC CTCAGGTTAG TACATTTCTG CACCTGGGAA 
AGGACCAAGA TGGAGCAAGA GCTGGGATTA GCGTCAAGCT TCATTAACAC AGCTGTATAC 
ACTGACAAAA GTTTGGGCAG GGACACGTTT GAGAGTGACC CGGAGAAACT CAGCAGCACG 
GACAATGAAG ATGAGGAAAT GGTGATCGAT GGCTCAAGGA CCCCCTGTAA AATACAAAGC 
TCTCGCTCGC ACAACTCAAC GTCATCTGTG TGTTCCAAAG CTTCAGAGGA GACACTAAGG 
CCACCATTGC AAGTGAGTGC TGCTAATGTG GAGAAGATGA AACACACAGT GGATAAAGGC 
ACCGGGAATC CCATCTCTTG TGAACAATCA GAGAGAACTA ACAGTCCTGA TCCAGCTGTG 
AAGAACACTC CAAGTTCAAC ATCTGCACCG TCCTCTTCCT CACCTTCCTG TTTCCCCAAT 
GGGATCGACC TGCAGACTTC ACAATGTTCT ATGTCCAAGG CATATAAGCA GCCGCCAATA 
GTTTTTCTCC CCAAACTGGT TTATGACATT ATTACCTCCA CTGACAGCAG TGGCCTTTCT 
AAATCTTCAT CTTTCCTGCC ACATTGTGAA GTCTCATGGG CCAGCTCTTT CCGCCCCCTT 
CTGAGTAAAA TGATGACTAG TACAGAGCAG TCTTTGTACT ACCGCCAATG GACGGTGCCC 
AAACAGAGCC ACATGGACTA CTGCAATATT AATGAAGGCA GAATGGATAC CTTCCACCCC 
AGGAGGCTGC TACTCAGTGG ACCACCTCAG ATAGGCAAAA CTGGAGCTTA TCTGCAGTTC 
CTCAGCGTCT TGTCCAGAAT GCTGATTAGA CTGACAGAGG TGGATGTATA TGATGAAGAA 
GAAATCAATA TCAGCCCCAG AGACAAATCC GACCAGCATT TCCACCAGAT TTTGGATACG 
TGGCCTAATG TGGAAACTAT AAAGGAGATG GCTTTTGATT ACACTTTACA TGACCCCAAG 
TATGAAGATG CCAGTGCCAT TTGCTCAGCT TTTCAGGATT TCCACACTGA AGGTAAACCA 
GTCCGAAAGA AGAATGAAGA TGTTTATATG AGGCGCCCCA CGACCAGAAT GAGGTTATCA 
AAGTATGCCT CCTACAACAC GTACCACCAT TGTGAGCAGT GCCACCAATA CATGGGATAC 
AACCCCAGGT TCCAGATGTA CGAATCCACC CTGCATGCTT TTGCATTCTC CTATTCACTG 
TTGGGTGAAG AAATTCAGCT GCACTTCATC ATACCCAAGT CCAAAGAACA TCACTTTGTC 
TTTAGCCAGC CGGGGGGCCA GCTGGAAAGC ATGAGGTTGC CATTGGTCGC AGATAAGAAT 
CCTGACTGTG TGAAGAGTCC CATCTTTACT CCAACCACAG GCCGCCATGA ACACGGGCTC 
TTCAATCTCT ATCATGCTAT GGATGGCAGC AGCCATTTGC ACATTTTGGT GGTCAAGGAA 
TATGAGATGG CGATATATAA AAAATACTGG CCAAACCACA TCATGTTGGT TCTCCCCAGT 
ATCTTTAACA GCGCCGGTGT AGGGGCAGCT CATTTCCTAA TCAAAGAGCT TTCTTATCAT 
AACCTGGAGC TGGAGCGCAA CAGACAAGGG GAGCTGGGCA TCAGGCCACA AGATGTCTGG 
CCTTTCGTCG TCATTGCTGA CGATTCGTGT GTCATGTGGA ACACTGTGGA TGTCAGCGTA 
ACGGGGGATC AACCTAGGGA TTCTTCATGT TCAGAAAGAA ACGTATCTTT GAAGTACATC 
CTTCAGCACA TGGAAGCCTC CCAGGACATT ACCCAGTATG CTCTGCTTGG AATGCGGAAG 
TGGACGAGTA AGAACAATGA AAGGGATATG AAGGAGCCTT TCTCCCGCTG CCATGTCCAT 
GATTTTGTCC TGCTCAACGT AAACCTGACG CAGGATGTAC AGTACAACCA AAACAGATTT 
ACCTGCGATG ATGTTGATTT CAATCTTCGG GTGAACAGCT CTGGACTGTT AATAGGCCGA 
TTCAACCGCT TCAGTGTTAT GAAAAAGCAG ATTGCAGTTG GTGGCCATAG ATCCCTACAT 
ATCAAATCAA AGGTTCTAGA CCCTTCAGTG TCCGTTTCCC CTGCTCAGTA CATCTGTGCC 
CCAGACAGCA AGCACACAAT TCTAGCTGCA CCGGCTCATC TGCTGCTCGA GAAATTCCTT 
CAGTTTCACA GCCACAGATT CTTTCCAATA TCACTCAACA ATAGCAGCCA CCCTGTGCTT 
ATAGTGGATT GCTACCTTAA CCTGGGGCCA CAGATAAGAG TTTGCTATGT GAGCTCTCGC 
CCTCACTCTA CCAACATTAG CTGCCCCGGA GTGCTCTTCA GCGGCCTCCT CCTGCACCTT 
TCTGACTCCT TTGTGGTTGC TGGGTTTTTG AAAAGGTTTC AGTTTCTGAA AGGGGCGACG 
TTGTGCGTGA CATGTCAGGA CCGCAACTCT CTGCGCCAGA CCGTCGTTCG GCTGGAACTG 
GAAGAAGAAT GGCAGTTCCG GCTACGAGAT GAATTCCAAA CCGCTAATGC TAAAGAAGAC 
AAGCCTCTGT TCATCCTTAC TGGCCGCCAT GTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

greb1 ( XM_012963456.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818910.1 Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X1, mRNA.

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CloneID	OXa32883
Clone ID Related Accession (Same CDS sequence)	XM_012963456.3 , XM_012963456.2 , XM_012963457.2 , XM_012963457.3
Accession Version	XM_012963456.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818910.1
CDS	139..5835
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTEVRSQNLSANMCSSTVPAMQDGPGSMNKSSDPPTAPNSYLNSGGVLTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPDGDLDFLLDKFYQENQGAATLSPIKPSTETPRPICTSYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_012963456.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963456.2

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

greb1 ( XM_012963457.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818911.1 Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X2, mRNA.

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CloneID	OXa32883
Clone ID Related Accession (Same CDS sequence)	XM_012963456.3 , XM_012963456.2 , XM_012963457.2 , XM_012963457.3
Accession Version	XM_012963457.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818911.1
CDS	134..5830
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTEVRSQNLSANMCSSTVPAMQDGPGSMNKSSDPPTAPNSYLNSGGVLTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPDGDLDFLLDKFYQENQGAATLSPIKPSTETPRPICTSYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_012963457.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963457.2

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGA TGGTGATTTA GATTTCCTGC TGGACAAGTT TTATCAGGAA 
AATCAAGGCG CTGCCACGTT ATCACCAATT AAGCCAAGCA CGGAAACACC ACGTCCTATA 
TGCACTAGTT ACAACACTGA GTGGCACCAT GTCCGCCCGT CCCAGCTGGC CGTGGCACAA 
AAGCTCTTAT CACATGTCTG CTCCATCGCC GACTCCAGCA CTCAGAACCT TGATTTGGGC 
TCATTTGAGA AAGTGGATTT TCTTATCTGT GTTCCTCCAT CGGAGATAAC CTACCAGCAA 
ACACTCTCTC ATCTGTGGAG TTCAGGGGTG CTACTGGAGC TTGGTGTGGA GAAGGCCTTG 
CTCACAAAGC AGCTGCTGGA GCAGTACATA ATGAAACTGG ATTCCGACGC GCCCAATAAA 
TTCAAGACGT TCCTCCACAG AGCCCTGCAG AACCCTCACA CCTTGTTTGT GCTCGTTCAT 
GACCACGCAC ATTGGGATCT CACCAGTTCT GTTTATAGCC CTTATGCTCA AAGTGAGTCC 
AACATGGGCC TTGTTGATCG ACTGCTAAAC TGCAGAGAAG TGAGAGACGC GCCCAATATC 
TTGGTTTTAC ACGTGACTTC CTTTCCTTAT GCTCTGCAAA CACAGAACAC CCGGATCAGC 
CCATACAATG AAATACACTG GCCTGCTTCA CACAGTCATG GGATGGAATT GTACCACGAG 
AGCAGGAAAT ACTTCGGTCT GTCCGAGTTT ATTGAATCAA CCGTTTCTGG CCACAGCCTT 
CCTTTGCTCC GCCATGACAG CTCCTTTGAA GCCATCGTAC TGGCACTAGG AAAGAGGTTC 
CCCAAGCTCC ACAGTGCCGT TATCCGGACG TTCCTTCTCA TCCAGCATTA CTCTGCTGCT 
ATGATGTCTG TCTGTGGTTT ATCGCAAATG AAGAATTACA CGTCTGTGGA AACACTGGAG 
ATCATTCAGA ACCTCCTGAG CTCCCCTCAG CAGTGTGCCA GTGCCCACGG CCTCATGGTC 
CTTTTAAGGA TCCCATGCAT CCCATTGGCC ACTCTTGCCT ACGAGCGCCT GTGTCACGTT 
CGAGAACGCC TCGGGCTGCA GCATCGATTT GAAATTATAA TGGCTACATC GAACTCAACC 
ATAAGTATTA GCAAGCACTT TGTGGACCAG CTACGGGCCT GGCAGAAGAA TGAAGATGCA 
AACTGGCAGC CACATACATA TCTGGAACTG GAAGGTCTGC CCTGTATCCT GATTTCTACT 
GGAATTGATC CCCTAGGAGA GTCTTTGCCA AGGTCATTGA GATACTGTGA CCTCAGGTTA 
GTACATTTCT GCACCTGGGA AAGGACCAAG ATGGAGCAAG AGCTGGGATT AGCGTCAAGC 
TTCATTAACA CAGCTGTATA CACTGACAAA AGTTTGGGCA GGGACACGTT TGAGAGTGAC 
CCGGAGAAAC TCAGCAGCAC GGACAATGAA GATGAGGAAA TGGTGATCGA TGGCTCAAGG 
ACCCCCTGTA AAATACAAAG CTCTCGCTCG CACAACTCAA CGTCATCTGT GTGTTCCAAA 
GCTTCAGAGG AGACACTAAG GCCACCATTG CAAGTGAGTG CTGCTAATGT GGAGAAGATG 
AAACACACAG TGGATAAAGG CACCGGGAAT CCCATCTCTT GTGAACAATC AGAGAGAACT 
AACAGTCCTG ATCCAGCTGT GAAGAACACT CCAAGTTCAA CATCTGCACC GTCCTCTTCC 
TCACCTTCCT GTTTCCCCAA TGGGATCGAC CTGCAGACTT CACAATGTTC TATGTCCAAG 
GCATATAAGC AGCCGCCAAT AGTTTTTCTC CCCAAACTGG TTTATGACAT TATTACCTCC 
ACTGACAGCA GTGGCCTTTC TAAATCTTCA TCTTTCCTGC CACATTGTGA AGTCTCATGG 
GCCAGCTCTT TCCGCCCCCT TCTGAGTAAA ATGATGACTA GTACAGAGCA GTCTTTGTAC 
TACCGCCAAT GGACGGTGCC CAAACAGAGC CACATGGACT ACTGCAATAT TAATGAAGGC 
AGAATGGATA CCTTCCACCC CAGGAGGCTG CTACTCAGTG GACCACCTCA GATAGGCAAA 
ACTGGAGCTT ATCTGCAGTT CCTCAGCGTC TTGTCCAGAA TGCTGATTAG ACTGACAGAG 
GTGGATGTAT ATGATGAAGA AGAAATCAAT ATCAGCCCCA GAGACAAATC CGACCAGCAT 
TTCCACCAGA TTTTGGATAC GTGGCCTAAT GTGGAAACTA TAAAGGAGAT GGCTTTTGAT 
TACACTTTAC ATGACCCCAA GTATGAAGAT GCCAGTGCCA TTTGCTCAGC TTTTCAGGAT 
TTCCACACTG AAGGTAAACC AGTCCGAAAG AAGAATGAAG ATGTTTATAT GAGGCGCCCC 
ACGACCAGAA TGAGGTTATC AAAGTATGCC TCCTACAACA CGTACCACCA TTGTGAGCAG 
TGCCACCAAT ACATGGGATA CAACCCCAGG TTCCAGATGT ACGAATCCAC CCTGCATGCT 
TTTGCATTCT CCTATTCACT GTTGGGTGAA GAAATTCAGC TGCACTTCAT CATACCCAAG 
TCCAAAGAAC ATCACTTTGT CTTTAGCCAG CCGGGGGGCC AGCTGGAAAG CATGAGGTTG 
CCATTGGTCG CAGATAAGAA TCCTGACTGT GTGAAGAGTC CCATCTTTAC TCCAACCACA 
GGCCGCCATG AACACGGGCT CTTCAATCTC TATCATGCTA TGGATGGCAG CAGCCATTTG 
CACATTTTGG TGGTCAAGGA ATATGAGATG GCGATATATA AAAAATACTG GCCAAACCAC 
ATCATGTTGG TTCTCCCCAG TATCTTTAAC AGCGCCGGTG TAGGGGCAGC TCATTTCCTA 
ATCAAAGAGC TTTCTTATCA TAACCTGGAG CTGGAGCGCA ACAGACAAGG GGAGCTGGGC 
ATCAGGCCAC AAGATGTCTG GCCTTTCGTC GTCATTGCTG ACGATTCGTG TGTCATGTGG 
AACACTGTGG ATGTCAGCGT AACGGGGGAT CAACCTAGGG ATTCTTCATG TTCAGAAAGA 
AACGTATCTT TGAAGTACAT CCTTCAGCAC ATGGAAGCCT CCCAGGACAT TACCCAGTAT 
GCTCTGCTTG GAATGCGGAA GTGGACGAGT AAGAACAATG AAAGGGATAT GAAGGAGCCT 
TTCTCCCGCT GCCATGTCCA TGATTTTGTC CTGCTCAACG TAAACCTGAC GCAGGATGTA 
CAGTACAACC AAAACAGATT TACCTGCGAT GATGTTGATT TCAATCTTCG GGTGAACAGC 
TCTGGACTGT TAATAGGCCG ATTCAACCGC TTCAGTGTTA TGAAAAAGCA GATTGCAGTT 
GGTGGCCATA GATCCCTACA TATCAAATCA AAGGTTCTAG ACCCTTCAGT GTCCGTTTCC 
CCTGCTCAGT ACATCTGTGC CCCAGACAGC AAGCACACAA TTCTAGCTGC ACCGGCTCAT 
CTGCTGCTCG AGAAATTCCT TCAGTTTCAC AGCCACAGAT TCTTTCCAAT ATCACTCAAC 
AATAGCAGCC ACCCTGTGCT TATAGTGGAT TGCTACCTTA ACCTGGGGCC ACAGATAAGA 
GTTTGCTATG TGAGCTCTCG CCCTCACTCT ACCAACATTA GCTGCCCCGG AGTGCTCTTC 
AGCGGCCTCC TCCTGCACCT TTCTGACTCC TTTGTGGTTG CTGGGTTTTT GAAAAGGTTT 
CAGTTTCTGA AAGGGGCGAC GTTGTGCGTG ACATGTCAGG ACCGCAACTC TCTGCGCCAG 
ACCGTCGTTC GGCTGGAACT GGAAGAAGAA TGGCAGTTCC GGCTACGAGA TGAATTCCAA 
ACCGCTAATG CTAAAGAAGA CAAGCCTCTG TTCATCCTTA CTGGCCGCCA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

greb1 ( XM_012963459.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818913.1 Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X3, mRNA.

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CloneID	OXa32884
Clone ID Related Accession (Same CDS sequence)	XM_012963459.2 , XM_012963459.3
Accession Version	XM_012963459.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGG TTACAACACT GAGTGGCACC ATGTCCGCCC GTCCCAGCTG 
GCCGTGGCAC AAAAGCTCTT ATCACATGTC TGCTCCATCG CCGACTCCAG CACTCAGAAC 
CTTGATTTGG GCTCATTTGA GAAAGTGGAT TTTCTTATCT GTGTTCCTCC ATCGGAGATA 
ACCTACCAGC AAACACTCTC TCATCTGTGG AGTTCAGGGG TGCTACTGGA GCTTGGTGTG 
GAGAAGGCCT TGCTCACAAA GCAGCTGCTG GAGCAGTACA TAATGAAACT GGATTCCGAC 
GCGCCCAATA AATTCAAGAC GTTCCTCCAC AGAGCCCTGC AGAACCCTCA CACCTTGTTT 
GTGCTCGTTC ATGACCACGC ACATTGGGAT CTCACCAGTT CTGTTTATAG CCCTTATGCT 
CAAAGTGAGT CCAACATGGG CCTTGTTGAT CGACTGCTAA ACTGCAGAGA AGTGAGAGAC 
GCGCCCAATA TCTTGGTTTT ACACGTGACT TCCTTTCCTT ATGCTCTGCA AACACAGAAC 
ACCCGGATCA GCCCATACAA TGAAATACAC TGGCCTGCTT CACACAGTCA TGGGATGGAA 
TTGTACCACG AGAGCAGGAA ATACTTCGGT CTGTCCGAGT TTATTGAATC AACCGTTTCT 
GGCCACAGCC TTCCTTTGCT CCGCCATGAC AGCTCCTTTG AAGCCATCGT ACTGGCACTA 
GGAAAGAGGT TCCCCAAGCT CCACAGTGCC GTTATCCGGA CGTTCCTTCT CATCCAGCAT 
TACTCTGCTG CTATGATGTC TGTCTGTGGT TTATCGCAAA TGAAGAATTA CACGTCTGTG 
GAAACACTGG AGATCATTCA GAACCTCCTG AGCTCCCCTC AGCAGTGTGC CAGTGCCCAC 
GGCCTCATGG TCCTTTTAAG GATCCCATGC ATCCCATTGG CCACTCTTGC CTACGAGCGC 
CTGTGTCACG TTCGAGAACG CCTCGGGCTG CAGCATCGAT TTGAAATTAT AATGGCTACA 
TCGAACTCAA CCATAAGTAT TAGCAAGCAC TTTGTGGACC AGCTACGGGC CTGGCAGAAG 
AATGAAGATG CAAACTGGCA GCCACATACA TATCTGGAAC TGGAAGGTCT GCCCTGTATC 
CTGATTTCTA CTGGAATTGA TCCCCTAGGA GAGTCTTTGC CAAGGTCATT GAGATACTGT 
GACCTCAGGT TAGTACATTT CTGCACCTGG GAAAGGACCA AGATGGAGCA AGAGCTGGGA 
TTAGCGTCAA GCTTCATTAA CACAGCTGTA TACACTGACA AAAGTTTGGG CAGGGACACG 
TTTGAGAGTG ACCCGGAGAA ACTCAGCAGC ACGGACAATG AAGATGAGGA AATGGTGATC 
GATGGCTCAA GGACCCCCTG TAAAATACAA AGCTCTCGCT CGCACAACTC AACGTCATCT 
GTGTGTTCCA AAGCTTCAGA GGAGACACTA AGGCCACCAT TGCAAGTGAG TGCTGCTAAT 
GTGGAGAAGA TGAAACACAC AGTGGATAAA GGCACCGGGA ATCCCATCTC TTGTGAACAA 
TCAGAGAGAA CTAACAGTCC TGATCCAGCT GTGAAGAACA CTCCAAGTTC AACATCTGCA 
CCGTCCTCTT CCTCACCTTC CTGTTTCCCC AATGGGATCG ACCTGCAGAC TTCACAATGT 
TCTATGTCCA AGGCATATAA GCAGCCGCCA ATAGTTTTTC TCCCCAAACT GGTTTATGAC 
ATTATTACCT CCACTGACAG CAGTGGCCTT TCTAAATCTT CATCTTTCCT GCCACATTGT 
GAAGTCTCAT GGGCCAGCTC TTTCCGCCCC CTTCTGAGTA AAATGATGAC TAGTACAGAG 
CAGTCTTTGT ACTACCGCCA ATGGACGGTG CCCAAACAGA GCCACATGGA CTACTGCAAT 
ATTAATGAAG GCAGAATGGA TACCTTCCAC CCCAGGAGGC TGCTACTCAG TGGACCACCT 
CAGATAGGCA AAACTGGAGC TTATCTGCAG TTCCTCAGCG TCTTGTCCAG AATGCTGATT 
AGACTGACAG AGGTGGATGT ATATGATGAA GAAGAAATCA ATATCAGCCC CAGAGACAAA 
TCCGACCAGC ATTTCCACCA GATTTTGGAT ACGTGGCCTA ATGTGGAAAC TATAAAGGAG 
ATGGCTTTTG ATTACACTTT ACATGACCCC AAGTATGAAG ATGCCAGTGC CATTTGCTCA 
GCTTTTCAGG ATTTCCACAC TGAAGGTAAA CCAGTCCGAA AGAAGAATGA AGATGTTTAT 
ATGAGGCGCC CCACGACCAG AATGAGGTTA TCAAAGTATG CCTCCTACAA CACGTACCAC 
CATTGTGAGC AGTGCCACCA ATACATGGGA TACAACCCCA GGTTCCAGAT GTACGAATCC 
ACCCTGCATG CTTTTGCATT CTCCTATTCA CTGTTGGGTG AAGAAATTCA GCTGCACTTC 
ATCATACCCA AGTCCAAAGA ACATCACTTT GTCTTTAGCC AGCCGGGGGG CCAGCTGGAA 
AGCATGAGGT TGCCATTGGT CGCAGATAAG AATCCTGACT GTGTGAAGAG TCCCATCTTT 
ACTCCAACCA CAGGCCGCCA TGAACACGGG CTCTTCAATC TCTATCATGC TATGGATGGC 
AGCAGCCATT TGCACATTTT GGTGGTCAAG GAATATGAGA TGGCGATATA TAAAAAATAC 
TGGCCAAACC ACATCATGTT GGTTCTCCCC AGTATCTTTA ACAGCGCCGG TGTAGGGGCA 
GCTCATTTCC TAATCAAAGA GCTTTCTTAT CATAACCTGG AGCTGGAGCG CAACAGACAA 
GGGGAGCTGG GCATCAGGCC ACAAGATGTC TGGCCTTTCG TCGTCATTGC TGACGATTCG 
TGTGTCATGT GGAACACTGT GGATGTCAGC GTAACGGGGG ATCAACCTAG GGATTCTTCA 
TGTTCAGAAA GAAACGTATC TTTGAAGTAC ATCCTTCAGC ACATGGAAGC CTCCCAGGAC 
ATTACCCAGT ATGCTCTGCT TGGAATGCGG AAGTGGACGA GTAAGAACAA TGAAAGGGAT 
ATGAAGGAGC CTTTCTCCCG CTGCCATGTC CATGATTTTG TCCTGCTCAA CGTAAACCTG 
ACGCAGGATG TACAGTACAA CCAAAACAGA TTTACCTGCG ATGATGTTGA TTTCAATCTT 
CGGGTGAACA GCTCTGGACT GTTAATAGGC CGATTCAACC GCTTCAGTGT TATGAAAAAG 
CAGATTGCAG TTGGTGGCCA TAGATCCCTA CATATCAAAT CAAAGGTTCT AGACCCTTCA 
GTGTCCGTTT CCCCTGCTCA GTACATCTGT GCCCCAGACA GCAAGCACAC AATTCTAGCT 
GCACCGGCTC ATCTGCTGCT CGAGAAATTC CTTCAGTTTC ACAGCCACAG ATTCTTTCCA 
ATATCACTCA ACAATAGCAG CCACCCTGTG CTTATAGTGG ATTGCTACCT TAACCTGGGG 
CCACAGATAA GAGTTTGCTA TGTGAGCTCT CGCCCTCACT CTACCAACAT TAGCTGCCCC 
GGAGTGCTCT TCAGCGGCCT CCTCCTGCAC CTTTCTGACT CCTTTGTGGT TGCTGGGTTT 
TTGAAAAGGT TTCAGTTTCT GAAAGGGGCG ACGTTGTGCG TGACATGTCA GGACCGCAAC 
TCTCTGCGCC AGACCGTCGT TCGGCTGGAA CTGGAAGAAG AATGGCAGTT CCGGCTACGA 
GATGAATTCC AAACCGCTAA TGCTAAAGAA GACAAGCCTC TGTTCATCCT TACTGGCCGC 
CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818913.1
CDS	140..5728
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNTLVPRPVFSQLYLEAEQQFSSLEGGRADNDDEEDDEDDGSGSSSPPLSYQSKPPPEGCCTTDGFCQAGKDLRLSSLSTDYFDVPPGFLLVGVKSPSLPDHILVCAVDKRFLPDDNGCNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLATQPKKQKHLKYYLIRNAQGNLTKGAIISWKGTEVRSQNLSANMCSSTVPAMQDGPGSMNKSSDPPTAPNSYLNSGGVLTGDNGYLQVSSSYQNMNGYLCPQQSAKVNPSAPARASVLGNLTNSAPPKKRHKGWSPESPTSTDASFAQSNFINSSICGKGDAMPQMCATQSTPGGLLSSSMVVRGESVSVPENLLKTSMIKPVIFKGHGSFPYLCGNVNDVIVSPLLHNCYTNSQSMPKAYEQYGPTTIQPISEEMQLLLTVYYLVQLAPDQVPLIEDLEQIFMRSWRETHLAEIRQYQQTVSQVFPHLPSPITPVTAAQLPWLAGLAASSCNESVKIIECSYSLAEGLCEMFKLLVEGKLSKTNYVVIICPSRSNGIDSCIVITGKYQARILVESMFSPSDYLKEVTYELASGKMENLAAFFHALCPGYNTEWHHVRPSQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICVPPSEITYQQTLSHLWSSGVLLELGVEKALLTKQLLEQYIMKLDSDAPNKFKTFLHRALQNPHTLFVLVHDHAHWDLTSSVYSPYAQSESNMGLVDRLLNCREVRDAPNILVLHVTSFPYALQTQNTRISPYNEIHWPASHSHGMELYHESRKYFGLSEFIESTVSGHSLPLLRHDSSFEAIVLALGKRFPKLHSAVIRTFLLIQHYSAAMMSVCGLSQMKNYTSVETLEIIQNLLSSPQQCASAHGLMVLLRIPCIPLATLAYERLCHVRERLGLQHRFEIIMATSNSTISISKHFVDQLRAWQKNEDANWQPHTYLELEGLPCILISTGIDPLGESLPRSLRYCDLRLVHFCTWERTKMEQELGLASSFINTAVYTDKSLGRDTFESDPEKLSSTDNEDEEMVIDGSRTPCKIQSSRSHNSTSSVCSKASEETLRPPLQVSAANVEKMKHTVDKGTGNPISCEQSERTNSPDPAVKNTPSSTSAPSSSSPSCFPNGIDLQTSQCSMSKAYKQPPIVFLPKLVYDIITSTDSSGLSKSSSFLPHCEVSWASSFRPLLSKMMTSTEQSLYYRQWTVPKQSHMDYCNINEGRMDTFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLIRLTEVDVYDEEEINISPRDKSDQHFHQILDTWPNVETIKEMAFDYTLHDPKYEDASAICSAFQDFHTEGKPVRKKNEDVYMRRPTTRMRLSKYASYNTYHHCEQCHQYMGYNPRFQMYESTLHAFAFSYSLLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVADKNPDCVKSPIFTPTTGRHEHGLFNLYHAMDGSSHLHILVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQGELGIRPQDVWPFVVIADDSCVMWNTVDVSVTGDQPRDSSCSERNVSLKYILQHMEASQDITQYALLGMRKWTSKNNERDMKEPFSRCHVHDFVLLNVNLTQDVQYNQNRFTCDDVDFNLRVNSSGLLIGRFNRFSVMKKQIAVGGHRSLHIKSKVLDPSVSVSPAQYICAPDSKHTILAAPAHLLLEKFLQFHSHRFFPISLNNSSHPVLIVDCYLNLGPQIRVCYVSSRPHSTNISCPGVLFSGLLLHLSDSFVVAGFLKRFQFLKGATLCVTCQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDKPLFILTGRHV

Target ORF information:

RefSeq Version	XM_012963459.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis growth regulation by estrogen in breast cancer 1 (greb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963459.2

ORF Insert Sequence:

ATGGGAAATT CGTATGCAGG GCAATTGAAG ACAACGAGGT TTGAAGAAGT TCTGCACAAT 
TCTATTGAGG CTTCCCTGAG ATCCAATACC CTGGTCCCCA GACCTGTGTT TTCCCAGCTG 
TATTTGGAAG CTGAACAGCA GTTTTCATCC CTGGAAGGTG GCCGAGCAGA CAATGATGAT 
GAAGAAGATG ATGAGGATGA TGGATCTGGC TCAAGCAGTC CACCCCTTTC TTACCAGAGT 
AAGCCACCCC CAGAAGGCTG TTGTACCACC GATGGATTTT GTCAGGCTGG GAAGGACCTA 
AGGCTCTCCT CACTATCTAC TGACTACTTT GATGTGCCAC CTGGATTTTT ATTGGTTGGC 
GTCAAGTCTC CCAGTCTACC TGATCACATA TTAGTGTGTG CAGTGGACAA GAGGTTTTTA 
CCAGATGATA ATGGATGCAA TGCACTACTA GGGTTTTCTG GCAACTGCGT TGGCTGTGGA 
GAAAAAGGCT TCCGATATTT CACAGAGTTT TCCAATCACA TCAACCTAAA GCTCGCGACT 
CAGCCCAAAA AGCAGAAACA CTTGAAGTAT TACCTCATCA GGAATGCGCA GGGGAACCTG 
ACCAAGGGAG CAATAATATC CTGGAAAGGC ACGGAGGTCA GAAGCCAGAA CCTTTCTGCT 
AACATGTGCT CAAGTACTGT ACCTGCTATG CAGGATGGAC CTGGATCCAT GAACAAGTCA 
AGTGACCCAC CTACTGCCCC CAATTCATAT CTGAACAGTG GGGGTGTCCT TACTGGAGAT 
AATGGCTACT TACAAGTCAG CTCCTCATAC CAGAATATGA ATGGGTACCT GTGCCCCCAG 
CAGTCTGCAA AGGTGAATCC CTCAGCACCA GCAAGGGCCT CGGTTTTAGG AAATTTGACA 
AATTCTGCCC CCCCAAAAAA ACGCCACAAA GGATGGTCTC CAGAGTCGCC AACATCAACA 
GATGCCAGCT TTGCACAGAG CAATTTTATT AACTCAAGTA TCTGTGGAAA AGGTGATGCC 
ATGCCTCAGA TGTGTGCCAC ACAATCCACC CCAGGAGGCC TTCTGTCTTC ATCCATGGTG 
GTGAGAGGAG AGTCTGTGTC TGTACCCGAG AACCTGCTGA AAACCAGCAT GATTAAGCCT 
GTCATTTTCA AGGGCCACGG GAGCTTCCCC TACCTCTGTG GAAATGTGAA TGATGTGATT 
GTGAGCCCTC TCCTGCACAA CTGCTACACA AACTCTCAGT CTATGCCCAA AGCCTATGAG 
CAGTACGGCC CCACCACAAT TCAGCCCATC TCAGAGGAAA TGCAGCTCCT GCTGACAGTC 
TATTATCTGG TCCAGCTTGC TCCAGACCAG GTTCCCTTGA TAGAGGACTT AGAGCAGATA 
TTCATGCGTT CATGGAGGGA AACTCACCTG GCTGAAATCC GCCAGTATCA GCAGACCGTG 
TCGCAGGTTT TCCCGCACCT TCCCAGCCCC ATAACTCCTG TGACTGCTGC GCAGCTTCCC 
TGGCTCGCCG GCCTCGCCGC CAGCTCCTGT AACGAGAGTG TGAAGATCAT TGAGTGCAGC 
TATTCCTTAG CGGAGGGGCT CTGTGAGATG TTCAAGCTGC TGGTGGAAGG GAAACTGTCA 
AAGACCAATT ATGTGGTAAT CATCTGCCCT TCCAGAAGCA ATGGTATAGA TTCCTGCATT 
GTAATAACAG GAAAATACCA GGCCCGGATT TTGGTAGAAA GCATGTTCTC TCCATCAGAT 
TACCTAAAAG AGGTGACGTA TGAATTAGCC AGCGGGAAGA TGGAGAATTT AGCAGCTTTT 
TTTCATGCAT TGTGCCCAGG TTACAACACT GAGTGGCACC ATGTCCGCCC GTCCCAGCTG 
GCCGTGGCAC AAAAGCTCTT ATCACATGTC TGCTCCATCG CCGACTCCAG CACTCAGAAC 
CTTGATTTGG GCTCATTTGA GAAAGTGGAT TTTCTTATCT GTGTTCCTCC ATCGGAGATA 
ACCTACCAGC AAACACTCTC TCATCTGTGG AGTTCAGGGG TGCTACTGGA GCTTGGTGTG 
GAGAAGGCCT TGCTCACAAA GCAGCTGCTG GAGCAGTACA TAATGAAACT GGATTCCGAC 
GCGCCCAATA AATTCAAGAC GTTCCTCCAC AGAGCCCTGC AGAACCCTCA CACCTTGTTT 
GTGCTCGTTC ATGACCACGC ACATTGGGAT CTCACCAGTT CTGTTTATAG CCCTTATGCT 
CAAAGTGAGT CCAACATGGG CCTTGTTGAT CGACTGCTAA ACTGCAGAGA AGTGAGAGAC 
GCGCCCAATA TCTTGGTTTT ACACGTGACT TCCTTTCCTT ATGCTCTGCA AACACAGAAC 
ACCCGGATCA GCCCATACAA TGAAATACAC TGGCCTGCTT CACACAGTCA TGGGATGGAA 
TTGTACCACG AGAGCAGGAA ATACTTCGGT CTGTCCGAGT TTATTGAATC AACCGTTTCT 
GGCCACAGCC TTCCTTTGCT CCGCCATGAC AGCTCCTTTG AAGCCATCGT ACTGGCACTA 
GGAAAGAGGT TCCCCAAGCT CCACAGTGCC GTTATCCGGA CGTTCCTTCT CATCCAGCAT 
TACTCTGCTG CTATGATGTC TGTCTGTGGT TTATCGCAAA TGAAGAATTA CACGTCTGTG 
GAAACACTGG AGATCATTCA GAACCTCCTG AGCTCCCCTC AGCAGTGTGC CAGTGCCCAC 
GGCCTCATGG TCCTTTTAAG GATCCCATGC ATCCCATTGG CCACTCTTGC CTACGAGCGC 
CTGTGTCACG TTCGAGAACG CCTCGGGCTG CAGCATCGAT TTGAAATTAT AATGGCTACA 
TCGAACTCAA CCATAAGTAT TAGCAAGCAC TTTGTGGACC AGCTACGGGC CTGGCAGAAG 
AATGAAGATG CAAACTGGCA GCCACATACA TATCTGGAAC TGGAAGGTCT GCCCTGTATC 
CTGATTTCTA CTGGAATTGA TCCCCTAGGA GAGTCTTTGC CAAGGTCATT GAGATACTGT 
GACCTCAGGT TAGTACATTT CTGCACCTGG GAAAGGACCA AGATGGAGCA AGAGCTGGGA 
TTAGCGTCAA GCTTCATTAA CACAGCTGTA TACACTGACA AAAGTTTGGG CAGGGACACG 
TTTGAGAGTG ACCCGGAGAA ACTCAGCAGC ACGGACAATG AAGATGAGGA AATGGTGATC 
GATGGCTCAA GGACCCCCTG TAAAATACAA AGCTCTCGCT CGCACAACTC AACGTCATCT 
GTGTGTTCCA AAGCTTCAGA GGAGACACTA AGGCCACCAT TGCAAGTGAG TGCTGCTAAT 
GTGGAGAAGA TGAAACACAC AGTGGATAAA GGCACCGGGA ATCCCATCTC TTGTGAACAA 
TCAGAGAGAA CTAACAGTCC TGATCCAGCT GTGAAGAACA CTCCAAGTTC AACATCTGCA 
CCGTCCTCTT CCTCACCTTC CTGTTTCCCC AATGGGATCG ACCTGCAGAC TTCACAATGT 
TCTATGTCCA AGGCATATAA GCAGCCGCCA ATAGTTTTTC TCCCCAAACT GGTTTATGAC 
ATTATTACCT CCACTGACAG CAGTGGCCTT TCTAAATCTT CATCTTTCCT GCCACATTGT 
GAAGTCTCAT GGGCCAGCTC TTTCCGCCCC CTTCTGAGTA AAATGATGAC TAGTACAGAG 
CAGTCTTTGT ACTACCGCCA ATGGACGGTG CCCAAACAGA GCCACATGGA CTACTGCAAT 
ATTAATGAAG GCAGAATGGA TACCTTCCAC CCCAGGAGGC TGCTACTCAG TGGACCACCT 
CAGATAGGCA AAACTGGAGC TTATCTGCAG TTCCTCAGCG TCTTGTCCAG AATGCTGATT 
AGACTGACAG AGGTGGATGT ATATGATGAA GAAGAAATCA ATATCAGCCC CAGAGACAAA 
TCCGACCAGC ATTTCCACCA GATTTTGGAT ACGTGGCCTA ATGTGGAAAC TATAAAGGAG 
ATGGCTTTTG ATTACACTTT ACATGACCCC AAGTATGAAG ATGCCAGTGC CATTTGCTCA 
GCTTTTCAGG ATTTCCACAC TGAAGGTAAA CCAGTCCGAA AGAAGAATGA AGATGTTTAT 
ATGAGGCGCC CCACGACCAG AATGAGGTTA TCAAAGTATG CCTCCTACAA CACGTACCAC 
CATTGTGAGC AGTGCCACCA ATACATGGGA TACAACCCCA GGTTCCAGAT GTACGAATCC 
ACCCTGCATG CTTTTGCATT CTCCTATTCA CTGTTGGGTG AAGAAATTCA GCTGCACTTC 
ATCATACCCA AGTCCAAAGA ACATCACTTT GTCTTTAGCC AGCCGGGGGG CCAGCTGGAA 
AGCATGAGGT TGCCATTGGT CGCAGATAAG AATCCTGACT GTGTGAAGAG TCCCATCTTT 
ACTCCAACCA CAGGCCGCCA TGAACACGGG CTCTTCAATC TCTATCATGC TATGGATGGC 
AGCAGCCATT TGCACATTTT GGTGGTCAAG GAATATGAGA TGGCGATATA TAAAAAATAC 
TGGCCAAACC ACATCATGTT GGTTCTCCCC AGTATCTTTA ACAGCGCCGG TGTAGGGGCA 
GCTCATTTCC TAATCAAAGA GCTTTCTTAT CATAACCTGG AGCTGGAGCG CAACAGACAA 
GGGGAGCTGG GCATCAGGCC ACAAGATGTC TGGCCTTTCG TCGTCATTGC TGACGATTCG 
TGTGTCATGT GGAACACTGT GGATGTCAGC GTAACGGGGG ATCAACCTAG GGATTCTTCA 
TGTTCAGAAA GAAACGTATC TTTGAAGTAC ATCCTTCAGC ACATGGAAGC CTCCCAGGAC 
ATTACCCAGT ATGCTCTGCT TGGAATGCGG AAGTGGACGA GTAAGAACAA TGAAAGGGAT 
ATGAAGGAGC CTTTCTCCCG CTGCCATGTC CATGATTTTG TCCTGCTCAA CGTAAACCTG 
ACGCAGGATG TACAGTACAA CCAAAACAGA TTTACCTGCG ATGATGTTGA TTTCAATCTT 
CGGGTGAACA GCTCTGGACT GTTAATAGGC CGATTCAACC GCTTCAGTGT TATGAAAAAG 
CAGATTGCAG TTGGTGGCCA TAGATCCCTA CATATCAAAT CAAAGGTTCT AGACCCTTCA 
GTGTCCGTTT CCCCTGCTCA GTACATCTGT GCCCCAGACA GCAAGCACAC AATTCTAGCT 
GCACCGGCTC ATCTGCTGCT CGAGAAATTC CTTCAGTTTC ACAGCCACAG ATTCTTTCCA 
ATATCACTCA ACAATAGCAG CCACCCTGTG CTTATAGTGG ATTGCTACCT TAACCTGGGG 
CCACAGATAA GAGTTTGCTA TGTGAGCTCT CGCCCTCACT CTACCAACAT TAGCTGCCCC 
GGAGTGCTCT TCAGCGGCCT CCTCCTGCAC CTTTCTGACT CCTTTGTGGT TGCTGGGTTT 
TTGAAAAGGT TTCAGTTTCT GAAAGGGGCG ACGTTGTGCG TGACATGTCA GGACCGCAAC 
TCTCTGCGCC AGACCGTCGT TCGGCTGGAA CTGGAAGAAG AATGGCAGTT CCGGCTACGA 
GATGAATTCC AAACCGCTAA TGCTAAAGAA GACAAGCCTC TGTTCATCCT TACTGGCCGC 
CATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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greb1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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