Greb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Greb1
Entrez Gene ID	500633
Full Name	growth regulation by estrogen in breast cancer 1
Synonyms	RGD1564091
General protein information	Preferred Names growth regulation by estrogen in breast cancer 1 Names protein GREB1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764638.1	XP_008762860.1	protein GREB1 isoform X1
XM_008776223.2	XP_008774445.1	protein GREB1 isoform X7
XM_008776225.2	XP_008774447.1	protein GREB1 isoform X8
XM_003754164.2	XP_003754212.1	protein GREB1 isoform X2
XM_006225731.1	XP_006225793.1	protein GREB1 isoform X3
XM_003754165.2	XP_003754213.1	protein GREB1 isoform X4
XM_008776221.2	XP_008774443.1	protein GREB1 isoform X5
XM_006225732.2	XP_006225794.1	protein GREB1 isoform X6

Pathways from BioSystems

Homologies

	GREB1	XP_001089374.1
Pan troglodytes (chimpanzee)	GREB1	XP_515304.3
Bos taurus (cattle)	GREB1	NP_001192560.1
Danio rerio (zebrafish)	greb1	XP_005158874.1
Homo sapiens (human)	GREB1	NP_055483.2
Canis lupus familiaris (dog)	GREB1	XP_005630186.1
Mus musculus (house mouse)	Greb1	NP_056579.2
Rattus norvegicus (Norway rat)	Greb1	XP_006239984.1
Gallus gallus (chicken)	GREB1	XP_004935825.1
Xenopus tropicalis (tropical clawed frog)	greb1	XP_002942230.2

Gene Ontology

The following Greb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Greb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa49407	XM_008776223.2 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa49409	XM_008776225.2 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa17142	XM_003754164.2 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa17143	XM_006225731.1 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa17144	XM_003754165.2 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa17145	XM_006225732.2 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa49405	XM_008776221.2 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa49410	XM_008764638.1 Latest version!	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Greb1 ( XM_008776223.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774445.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa49407
Clone ID Related Accession (Same CDS sequence)	XM_008776223.2
Accession Version	XM_008776223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776223.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGCACTA TGCGGCCGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774445.1
CDS	96..2789
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKSTMRPP

Target ORF information:

RefSeq Version	XM_008776223.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776223.2

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGCACTA TGCGGCCGCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_008776225.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774447.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa49409
Clone ID Related Accession (Same CDS sequence)	XM_008776225.2
Accession Version	XM_008776225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG TGCTGTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774447.1
CDS	96..2357
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQCCS

Target ORF information:

RefSeq Version	XM_008776225.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776225.2

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG TGCTGTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_003754164.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003754212.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X1, mRNA.

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CloneID	ORa17142
Clone ID Related Accession (Same CDS sequence)	XM_003754164.2
Accession Version	XM_003754164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2013 this sequence version replaced XM_003754164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCACAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCAAAGC AGTGCCCCTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCT 
CCTCTGGCAG CTGTGGCCTA TGAGCGACTG GCCCATGTGC GGGCACGGTT GGCTCTAGAA 
GAACACTTTG AGATCATTCT GGGCCACCCC AGCTCCGGCA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACTTAC 
TTGGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCGCTGCCAC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAATTCTTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA ACTGGGTCTG GCTGCCTACT TTGTTAGCAA CGATATCCCC 
TTGGAGAAGG GGCCCAAGAA TGAGGCCTTG GAGAGTGACG GGGAGAAGCT GAGTAGCACA 
GACGAAGATG AGGAGGCAGG GACAGAAGGG TGTTTTGAAG CAGGCTCCAC CTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGTTCCCAC TCCCATGACT CCGCATCTTC ATCTCTCTCT 
TCCAAAGCGT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG TTCAGCCCTC AGGACTTCCA 
CAGGGAGAGT TTGCCCGGTC ATCTCCACCC TGTGGCCCTG CAGAGGAGGG CAGGGCCCCT 
GGGGAGATAC AGCGGCTCCG AGTGAGTCAG GGACCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGAC GGAGGAGCCT GCAGGTGCCA 
GCCGCCCCAT CCTCCCAGCT GTCCTCCTCC TCCTCGGGCG CATCATCCAC ATGCACGGTG 
TCCACTGCCA ATGTGCTAGT CCTTCAAGCT TCTCAGTGCT CCATGGCCAA GGCCTGCCGG 
CAGCCACCCA TTGTCTTCCT GCCTAAGCTC GTGTATGACA TGCTCCTGTC CACTGACAGC 
AGTGGCTTGC CCAAGTCTGC CTCCCTCCTG CCTTCCCCTT CGGTTATGTG GACCAGCTCC 
TTCCGCCCCC TCCTCAGCAA GATGATGACG TCAACTGAAC AGTCCCTCTA CTACCGGCAG 
TGGACAGTGC CCCGGCCCAG CCACATGGAC TACGGCAACA GAGCTGAAGG CCGTGTGGAC 
AGTTTCCACC CACGTAGGCT GCTGCTCAGT GGGCCACCTC AGATTGGGAA GACGGGTGCC 
TACCTGCAGT TCCTCAGTAT CTTGTCCAGG ATGCTGATTC GGCTGACAGA AGTGGATGTT 
TACGATGAAG AAGAGATCAA TACCGGCCTC CAAGAGGAAT CAGATTGGCA TTACCTCCAG 
CTCACCGACC CTTGGCCTGA CCTGGAGCTC TTCCAGAAGA TGCCTTTTGA TTACATCATT 
CATGACCCGA AGTATGAGGA TGCCAGTATG ATTTGTTCAC ACCACCAGAG CATCAAGAGT 
GAAGACAGAG GGATGTCCAG GAAGCCAGAG GACCTGTATG TGCGGCGTCA GACGGCACGT 
ATGAGACTGT CTAAGTATGC GGCATACAAC ACATACCACC ATTGCGAGCA GTGCCAGCAG 
TACATGGGCT TCCACCCCCA CTACCAGCTC TCTGAGTCCA CCCTGCACGT CTTTGCCTTC 
TCTTGCTCCA TGCTAGGAGA GGAGATCCAG CTCCACTTCA TCATCCCCAA GTCCAAGGAG 
TACCACTTTG TCTTCAGCCA ACCGGGAGGC CAGCTGGAGA GCATGCGCTT GCCCCTCGTC 
ACAGACAAGA GTTATGAGCA CATCAAAAGC CCGACCTTCA CCCCGACGAC AGGCCGGCAT 
GAGCATGGGC TCTTTAATTT GTACCATGCG ATGGATGGAG CCAACCATCT ACATGTGCTG 
GTGGTCAAAG AGTACGAAAT GGCCATTTAT AAGAAGTACT GGCCCAACCA CATCATGCTT 
GTCCTCCCAA GCATCTTTAA CAGTGCTGGA GTCGGGGCTG CCCACTTCCT GATCAAAGAG 
CTGTGCTACC ACAACCTGGA GCTGGAGAGG AACCGGCAGG AGGAGCTGGG GGTCAAGCCT 
CAGGACATCT GGCCCTTCAT CGTCATCTCC GATGACTCCT GTGTGATGTG GAATGTTGCA 
GACGTGGACT GTGCTGGAGA CCGGAGCAGG GAGTTTTCCT GGTCAGAGAG GAATGTCTCT 
CTGAAACACA TCATGCAGCA CATTGAGGCG TCCCCTAACA TCACACACTA CGCCCTGATC 
GGCATGCGGA AGTGGGCCAG CAAGACCCGG AGCCGTGAGG TGCAGGAGCC TTTTTCCCGA 
TGCCACGTGC ATGACTTCAT CATCCTCAAC GTAGACTTGA CCCAGAATGT ACAGTACAAC 
CAGAATCGGT TTACATGTGA CGACGTAGAC TTCAACCTTC GCGTGCACAG TGCTGGCCTC 
CTGCTCTGCC GGTTCAATCG CTTCAGCGTC ATGAAGAAGC AGATCGCCGT GGGTGGGCAC 
CGGTCCTTCC ACATCACATC CAAGGTGGCT GACAGCTCTG TGGCCATTGT GCCTTCCCAG 
TACATCTGTG CCCCGGACAG TAAGCACACA TTCCTGGCAG CTCCCGCCCA GCTCCTGCTG 
GAGAAGTTCC TCCAGTACCA CAGCCACCGC TTCTTCCCGC TGTCTCTGAA GAACCACAGC 
CACCCCGTGT TGTCTGTAGA CTGCTACCTT AACCTGGGGC CTCAGATTTC TGTTTGTTAT 
GTCAGCTCCA GGCCCCACTC CTTAAACGTC AGCTGCTCTG ACATGATGTT CAGCGGGCTC 
CTGCTGTACC TCTGCGACTC TTTTGTGGGG GCTAGCTTCT TGAAGAAGTT CCATTTTCTG 
AAAGGAGCCA CCTTGTGCGT GATCTGCCAA GACCGGAACT CGCTTCGACA GACGGTGGTC 
CGCCTGGAGC TAGAAGAGGA GTGGCAGTTC CGGCTGCGGG ATGAGTTCCA GACAGCCAAT 
GCCAAGGAAG ACAGGCCACT CTTTTTCCTC ACTGCAAGAC ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003754212.1
CDS	96..5963
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSKASGSVLYGESLVQPSGLPQGEFARSSPPCGPAEEGRAPGEIQRLRVSQGPTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSSGASSTCTVSTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTGLQEESDWHYLQLTDPWPDLELFQKMPFDYIIHDPKYEDASMICSHHQSIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEIQLHFIIPKSKEYHFVFSQPGGQLESMRLPLVTDKSYEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDIWPFIVISDDSCVMWNVADVDCAGDRSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWASKTRSREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVADSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNVSCSDMMFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_003754164.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003754164.2

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCACAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCAAAGC AGTGCCCCTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCT 
CCTCTGGCAG CTGTGGCCTA TGAGCGACTG GCCCATGTGC GGGCACGGTT GGCTCTAGAA 
GAACACTTTG AGATCATTCT GGGCCACCCC AGCTCCGGCA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACTTAC 
TTGGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCGCTGCCAC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAATTCTTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA ACTGGGTCTG GCTGCCTACT TTGTTAGCAA CGATATCCCC 
TTGGAGAAGG GGCCCAAGAA TGAGGCCTTG GAGAGTGACG GGGAGAAGCT GAGTAGCACA 
GACGAAGATG AGGAGGCAGG GACAGAAGGG TGTTTTGAAG CAGGCTCCAC CTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGTTCCCAC TCCCATGACT CCGCATCTTC ATCTCTCTCT 
TCCAAAGCGT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG TTCAGCCCTC AGGACTTCCA 
CAGGGAGAGT TTGCCCGGTC ATCTCCACCC TGTGGCCCTG CAGAGGAGGG CAGGGCCCCT 
GGGGAGATAC AGCGGCTCCG AGTGAGTCAG GGACCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGAC GGAGGAGCCT GCAGGTGCCA 
GCCGCCCCAT CCTCCCAGCT GTCCTCCTCC TCCTCGGGCG CATCATCCAC ATGCACGGTG 
TCCACTGCCA ATGTGCTAGT CCTTCAAGCT TCTCAGTGCT CCATGGCCAA GGCCTGCCGG 
CAGCCACCCA TTGTCTTCCT GCCTAAGCTC GTGTATGACA TGCTCCTGTC CACTGACAGC 
AGTGGCTTGC CCAAGTCTGC CTCCCTCCTG CCTTCCCCTT CGGTTATGTG GACCAGCTCC 
TTCCGCCCCC TCCTCAGCAA GATGATGACG TCAACTGAAC AGTCCCTCTA CTACCGGCAG 
TGGACAGTGC CCCGGCCCAG CCACATGGAC TACGGCAACA GAGCTGAAGG CCGTGTGGAC 
AGTTTCCACC CACGTAGGCT GCTGCTCAGT GGGCCACCTC AGATTGGGAA GACGGGTGCC 
TACCTGCAGT TCCTCAGTAT CTTGTCCAGG ATGCTGATTC GGCTGACAGA AGTGGATGTT 
TACGATGAAG AAGAGATCAA TACCGGCCTC CAAGAGGAAT CAGATTGGCA TTACCTCCAG 
CTCACCGACC CTTGGCCTGA CCTGGAGCTC TTCCAGAAGA TGCCTTTTGA TTACATCATT 
CATGACCCGA AGTATGAGGA TGCCAGTATG ATTTGTTCAC ACCACCAGAG CATCAAGAGT 
GAAGACAGAG GGATGTCCAG GAAGCCAGAG GACCTGTATG TGCGGCGTCA GACGGCACGT 
ATGAGACTGT CTAAGTATGC GGCATACAAC ACATACCACC ATTGCGAGCA GTGCCAGCAG 
TACATGGGCT TCCACCCCCA CTACCAGCTC TCTGAGTCCA CCCTGCACGT CTTTGCCTTC 
TCTTGCTCCA TGCTAGGAGA GGAGATCCAG CTCCACTTCA TCATCCCCAA GTCCAAGGAG 
TACCACTTTG TCTTCAGCCA ACCGGGAGGC CAGCTGGAGA GCATGCGCTT GCCCCTCGTC 
ACAGACAAGA GTTATGAGCA CATCAAAAGC CCGACCTTCA CCCCGACGAC AGGCCGGCAT 
GAGCATGGGC TCTTTAATTT GTACCATGCG ATGGATGGAG CCAACCATCT ACATGTGCTG 
GTGGTCAAAG AGTACGAAAT GGCCATTTAT AAGAAGTACT GGCCCAACCA CATCATGCTT 
GTCCTCCCAA GCATCTTTAA CAGTGCTGGA GTCGGGGCTG CCCACTTCCT GATCAAAGAG 
CTGTGCTACC ACAACCTGGA GCTGGAGAGG AACCGGCAGG AGGAGCTGGG GGTCAAGCCT 
CAGGACATCT GGCCCTTCAT CGTCATCTCC GATGACTCCT GTGTGATGTG GAATGTTGCA 
GACGTGGACT GTGCTGGAGA CCGGAGCAGG GAGTTTTCCT GGTCAGAGAG GAATGTCTCT 
CTGAAACACA TCATGCAGCA CATTGAGGCG TCCCCTAACA TCACACACTA CGCCCTGATC 
GGCATGCGGA AGTGGGCCAG CAAGACCCGG AGCCGTGAGG TGCAGGAGCC TTTTTCCCGA 
TGCCACGTGC ATGACTTCAT CATCCTCAAC GTAGACTTGA CCCAGAATGT ACAGTACAAC 
CAGAATCGGT TTACATGTGA CGACGTAGAC TTCAACCTTC GCGTGCACAG TGCTGGCCTC 
CTGCTCTGCC GGTTCAATCG CTTCAGCGTC ATGAAGAAGC AGATCGCCGT GGGTGGGCAC 
CGGTCCTTCC ACATCACATC CAAGGTGGCT GACAGCTCTG TGGCCATTGT GCCTTCCCAG 
TACATCTGTG CCCCGGACAG TAAGCACACA TTCCTGGCAG CTCCCGCCCA GCTCCTGCTG 
GAGAAGTTCC TCCAGTACCA CAGCCACCGC TTCTTCCCGC TGTCTCTGAA GAACCACAGC 
CACCCCGTGT TGTCTGTAGA CTGCTACCTT AACCTGGGGC CTCAGATTTC TGTTTGTTAT 
GTCAGCTCCA GGCCCCACTC CTTAAACGTC AGCTGCTCTG ACATGATGTT CAGCGGGCTC 
CTGCTGTACC TCTGCGACTC TTTTGTGGGG GCTAGCTTCT TGAAGAAGTT CCATTTTCTG 
AAAGGAGCCA CCTTGTGCGT GATCTGCCAA GACCGGAACT CGCTTCGACA GACGGTGGTC 
CGCCTGGAGC TAGAAGAGGA GTGGCAGTTC CGGCTGCGGG ATGAGTTCCA GACAGCCAAT 
GCCAAGGAAG ACAGGCCACT CTTTTTCCTC ACTGCAAGAC ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006225731.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006225793.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X2, mRNA.

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CloneID	ORa17143
Clone ID Related Accession (Same CDS sequence)	XM_006225731.1
Accession Version	XM_006225731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGTGCTGTTC ATAACATCTA TTCTCAAAGT GACCCATCTG TGGGATTAGT GGACAGACTG 
CTCAACTGCC GAGAGGTGAA AGAGGCGCCC AACATTGTGA CTCTCCATGT GACCTCCTTC 
CCGTATGCGC TGCAGACTCA GCACACGCTC ATCAGCCCCT ACAATGAGAT CCACTGGCCC 
ATCTCCTACA GCAATGGAGT GGACCTGTAT CATGAGAGTA AGAAGTACTT TGGGCTGTCT 
GAGTTTATTG ACTCCACCCT GTCAGGACAC AGTCTTCCTC TGCTCCGGTA TGACAGCTCC 
TTTGAGGCCA TGGTCACTGC ATTGGGGAAA AGGTTCCCCC GCCTGCACAG CGCGGTGATC 
AGGACCTTTG TTCTTGTGCA GCACTATGCG GCCGCCATGA TGGCCGTGAG TGGCCTCCCA 
CAGATGAAGA ACCATACATC AGTGGAGACC CTAGAGATCA CCCAGAACCT CCTCAACTCC 
CCAAAGCAGT GCCCCTGTGG CCACGGGCTC ATGGTCCTGC TGCGCGTGCC CTGTTCTCCT 
CTGGCAGCTG TGGCCTATGA GCGACTGGCC CATGTGCGGG CACGGTTGGC TCTAGAAGAA 
CACTTTGAGA TCATTCTGGG CCACCCCAGC TCCGGCATCA CTGTAGGCAA GCACTTTGTG 
AAGCAGCTCA AGATGTGGCA GAAAATTGAG GATGCAGAAT GGAGGCCTCA GACTTACTTG 
GAGTTGGAGG GCCTGCCCTG CATCCTGATC TTCAGTGGGA TGGACCCTCA TGGGGAGTCG 
CTGCCACGGT CTCTGAGGTA CTGTGACCTG CGTCTAATAA ATTCTTCCTG CCTGGTGAGG 
ACAGCCCTGG AGCAGGAACT GGGTCTGGCT GCCTACTTTG TTAGCAACGA TATCCCCTTG 
GAGAAGGGGC CCAAGAATGA GGCCTTGGAG AGTGACGGGG AGAAGCTGAG TAGCACAGAC 
GAAGATGAGG AGGCAGGGAC AGAAGGGTGT TTTGAAGCAG GCTCCACCTC AGAGCAGAGG 
GGCCCTGTGA AGAGGGAACG TTCCCACTCC CATGACTCCG CATCTTCATC TCTCTCTTCC 
AAAGCGTCTG GCTCGGTGCT CTATGGTGAG TCCCTGGTTC AGCCCTCAGG ACTTCCACAG 
GGAGAGTTTG CCCGGTCATC TCCACCCTGT GGCCCTGCAG AGGAGGGCAG GGCCCCTGGG 
GAGATACAGC GGCTCCGAGT GAGTCAGGGA CCTACAGTCA TCAGTAGGCA CAGCCCTGGC 
CTGGTCCCAC AGCCTGATTC TAGCCTCAGG ACTGGACGGA GGAGCCTGCA GGTGCCAGCC 
GCCCCATCCT CCCAGCTGTC CTCCTCCTCC TCGGGCGCAT CATCCACATG CACGGTGTCC 
ACTGCCAATG TGCTAGTCCT TCAAGCTTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATTG TCTTCCTGCC TAAGCTCGTG TATGACATGC TCCTGTCCAC TGACAGCAGT 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCGG TTATGTGGAC CAGCTCCTTC 
CGCCCCCTCC TCAGCAAGAT GATGACGTCA ACTGAACAGT CCCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAAGGCCG TGTGGACAGT 
TTCCACCCAC GTAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC GGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GATGAAGAAG AGATCAATAC CGGCCTCCAA GAGGAATCAG ATTGGCATTA CCTCCAGCTC 
ACCGACCCTT GGCCTGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGATGC CAGTATGATT TGTTCACACC ACCAGAGCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCAGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCTA AGTATGCGGC ATACAACACA TACCACCATT GCGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GATCCAGCTC CACTTCATCA TCCCCAAGTC CAAGGAGTAC 
CACTTTGTCT TCAGCCAACC GGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTT ATGAGCACAT CAAAAGCCCG ACCTTCACCC CGACGACAGG CCGGCATGAG 
CATGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCATCTACA TGTGCTGGTG 
GTCAAAGAGT ACGAAATGGC CATTTATAAG AAGTACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGAGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTG 
TGCTACCACA ACCTGGAGCT GGAGAGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACATCTGGC CCTTCATCGT CATCTCCGAT GACTCCTGTG TGATGTGGAA TGTTGCAGAC 
GTGGACTGTG CTGGAGACCG GAGCAGGGAG TTTTCCTGGT CAGAGAGGAA TGTCTCTCTG 
AAACACATCA TGCAGCACAT TGAGGCGTCC CCTAACATCA CACACTACGC CCTGATCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGAGC CGTGAGGTGC AGGAGCCTTT TTCCCGATGC 
CACGTGCATG ACTTCATCAT CCTCAACGTA GACTTGACCC AGAATGTACA GTACAACCAG 
AATCGGTTTA CATGTGACGA CGTAGACTTC AACCTTCGCG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGCGTCATG AAGAAGCAGA TCGCCGTGGG TGGGCACCGG 
TCCTTCCACA TCACATCCAA GGTGGCTGAC AGCTCTGTGG CCATTGTGCC TTCCCAGTAC 
ATCTGTGCCC CGGACAGTAA GCACACATTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAGTTCCTCC AGTACCACAG CCACCGCTTC TTCCCGCTGT CTCTGAAGAA CCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTTAAC CTGGGGCCTC AGATTTCTGT TTGTTATGTC 
AGCTCCAGGC CCCACTCCTT AAACGTCAGC TGCTCTGACA TGATGTTCAG CGGGCTCCTG 
CTGTACCTCT GCGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGCGTGAT CTGCCAAGAC CGGAACTCGC TTCGACAGAC GGTGGTCCGC 
CTGGAGCTAG AAGAGGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCACTCTT TTTCCTCACT GCAAGACACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006225793.1
CDS	96..5960
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSKASGSVLYGESLVQPSGLPQGEFARSSPPCGPAEEGRAPGEIQRLRVSQGPTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSSGASSTCTVSTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTGLQEESDWHYLQLTDPWPDLELFQKMPFDYIIHDPKYEDASMICSHHQSIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEIQLHFIIPKSKEYHFVFSQPGGQLESMRLPLVTDKSYEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDIWPFIVISDDSCVMWNVADVDCAGDRSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWASKTRSREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVADSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNVSCSDMMFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_006225731.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006225731.1

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGTGCTGTTC ATAACATCTA TTCTCAAAGT GACCCATCTG TGGGATTAGT GGACAGACTG 
CTCAACTGCC GAGAGGTGAA AGAGGCGCCC AACATTGTGA CTCTCCATGT GACCTCCTTC 
CCGTATGCGC TGCAGACTCA GCACACGCTC ATCAGCCCCT ACAATGAGAT CCACTGGCCC 
ATCTCCTACA GCAATGGAGT GGACCTGTAT CATGAGAGTA AGAAGTACTT TGGGCTGTCT 
GAGTTTATTG ACTCCACCCT GTCAGGACAC AGTCTTCCTC TGCTCCGGTA TGACAGCTCC 
TTTGAGGCCA TGGTCACTGC ATTGGGGAAA AGGTTCCCCC GCCTGCACAG CGCGGTGATC 
AGGACCTTTG TTCTTGTGCA GCACTATGCG GCCGCCATGA TGGCCGTGAG TGGCCTCCCA 
CAGATGAAGA ACCATACATC AGTGGAGACC CTAGAGATCA CCCAGAACCT CCTCAACTCC 
CCAAAGCAGT GCCCCTGTGG CCACGGGCTC ATGGTCCTGC TGCGCGTGCC CTGTTCTCCT 
CTGGCAGCTG TGGCCTATGA GCGACTGGCC CATGTGCGGG CACGGTTGGC TCTAGAAGAA 
CACTTTGAGA TCATTCTGGG CCACCCCAGC TCCGGCATCA CTGTAGGCAA GCACTTTGTG 
AAGCAGCTCA AGATGTGGCA GAAAATTGAG GATGCAGAAT GGAGGCCTCA GACTTACTTG 
GAGTTGGAGG GCCTGCCCTG CATCCTGATC TTCAGTGGGA TGGACCCTCA TGGGGAGTCG 
CTGCCACGGT CTCTGAGGTA CTGTGACCTG CGTCTAATAA ATTCTTCCTG CCTGGTGAGG 
ACAGCCCTGG AGCAGGAACT GGGTCTGGCT GCCTACTTTG TTAGCAACGA TATCCCCTTG 
GAGAAGGGGC CCAAGAATGA GGCCTTGGAG AGTGACGGGG AGAAGCTGAG TAGCACAGAC 
GAAGATGAGG AGGCAGGGAC AGAAGGGTGT TTTGAAGCAG GCTCCACCTC AGAGCAGAGG 
GGCCCTGTGA AGAGGGAACG TTCCCACTCC CATGACTCCG CATCTTCATC TCTCTCTTCC 
AAAGCGTCTG GCTCGGTGCT CTATGGTGAG TCCCTGGTTC AGCCCTCAGG ACTTCCACAG 
GGAGAGTTTG CCCGGTCATC TCCACCCTGT GGCCCTGCAG AGGAGGGCAG GGCCCCTGGG 
GAGATACAGC GGCTCCGAGT GAGTCAGGGA CCTACAGTCA TCAGTAGGCA CAGCCCTGGC 
CTGGTCCCAC AGCCTGATTC TAGCCTCAGG ACTGGACGGA GGAGCCTGCA GGTGCCAGCC 
GCCCCATCCT CCCAGCTGTC CTCCTCCTCC TCGGGCGCAT CATCCACATG CACGGTGTCC 
ACTGCCAATG TGCTAGTCCT TCAAGCTTCT CAGTGCTCCA TGGCCAAGGC CTGCCGGCAG 
CCACCCATTG TCTTCCTGCC TAAGCTCGTG TATGACATGC TCCTGTCCAC TGACAGCAGT 
GGCTTGCCCA AGTCTGCCTC CCTCCTGCCT TCCCCTTCGG TTATGTGGAC CAGCTCCTTC 
CGCCCCCTCC TCAGCAAGAT GATGACGTCA ACTGAACAGT CCCTCTACTA CCGGCAGTGG 
ACAGTGCCCC GGCCCAGCCA CATGGACTAC GGCAACAGAG CTGAAGGCCG TGTGGACAGT 
TTCCACCCAC GTAGGCTGCT GCTCAGTGGG CCACCTCAGA TTGGGAAGAC GGGTGCCTAC 
CTGCAGTTCC TCAGTATCTT GTCCAGGATG CTGATTCGGC TGACAGAAGT GGATGTTTAC 
GATGAAGAAG AGATCAATAC CGGCCTCCAA GAGGAATCAG ATTGGCATTA CCTCCAGCTC 
ACCGACCCTT GGCCTGACCT GGAGCTCTTC CAGAAGATGC CTTTTGATTA CATCATTCAT 
GACCCGAAGT ATGAGGATGC CAGTATGATT TGTTCACACC ACCAGAGCAT CAAGAGTGAA 
GACAGAGGGA TGTCCAGGAA GCCAGAGGAC CTGTATGTGC GGCGTCAGAC GGCACGTATG 
AGACTGTCTA AGTATGCGGC ATACAACACA TACCACCATT GCGAGCAGTG CCAGCAGTAC 
ATGGGCTTCC ACCCCCACTA CCAGCTCTCT GAGTCCACCC TGCACGTCTT TGCCTTCTCT 
TGCTCCATGC TAGGAGAGGA GATCCAGCTC CACTTCATCA TCCCCAAGTC CAAGGAGTAC 
CACTTTGTCT TCAGCCAACC GGGAGGCCAG CTGGAGAGCA TGCGCTTGCC CCTCGTCACA 
GACAAGAGTT ATGAGCACAT CAAAAGCCCG ACCTTCACCC CGACGACAGG CCGGCATGAG 
CATGGGCTCT TTAATTTGTA CCATGCGATG GATGGAGCCA ACCATCTACA TGTGCTGGTG 
GTCAAAGAGT ACGAAATGGC CATTTATAAG AAGTACTGGC CCAACCACAT CATGCTTGTC 
CTCCCAAGCA TCTTTAACAG TGCTGGAGTC GGGGCTGCCC ACTTCCTGAT CAAAGAGCTG 
TGCTACCACA ACCTGGAGCT GGAGAGGAAC CGGCAGGAGG AGCTGGGGGT CAAGCCTCAG 
GACATCTGGC CCTTCATCGT CATCTCCGAT GACTCCTGTG TGATGTGGAA TGTTGCAGAC 
GTGGACTGTG CTGGAGACCG GAGCAGGGAG TTTTCCTGGT CAGAGAGGAA TGTCTCTCTG 
AAACACATCA TGCAGCACAT TGAGGCGTCC CCTAACATCA CACACTACGC CCTGATCGGC 
ATGCGGAAGT GGGCCAGCAA GACCCGGAGC CGTGAGGTGC AGGAGCCTTT TTCCCGATGC 
CACGTGCATG ACTTCATCAT CCTCAACGTA GACTTGACCC AGAATGTACA GTACAACCAG 
AATCGGTTTA CATGTGACGA CGTAGACTTC AACCTTCGCG TGCACAGTGC TGGCCTCCTG 
CTCTGCCGGT TCAATCGCTT CAGCGTCATG AAGAAGCAGA TCGCCGTGGG TGGGCACCGG 
TCCTTCCACA TCACATCCAA GGTGGCTGAC AGCTCTGTGG CCATTGTGCC TTCCCAGTAC 
ATCTGTGCCC CGGACAGTAA GCACACATTC CTGGCAGCTC CCGCCCAGCT CCTGCTGGAG 
AAGTTCCTCC AGTACCACAG CCACCGCTTC TTCCCGCTGT CTCTGAAGAA CCACAGCCAC 
CCCGTGTTGT CTGTAGACTG CTACCTTAAC CTGGGGCCTC AGATTTCTGT TTGTTATGTC 
AGCTCCAGGC CCCACTCCTT AAACGTCAGC TGCTCTGACA TGATGTTCAG CGGGCTCCTG 
CTGTACCTCT GCGACTCTTT TGTGGGGGCT AGCTTCTTGA AGAAGTTCCA TTTTCTGAAA 
GGAGCCACCT TGTGCGTGAT CTGCCAAGAC CGGAACTCGC TTCGACAGAC GGTGGTCCGC 
CTGGAGCTAG AAGAGGAGTG GCAGTTCCGG CTGCGGGATG AGTTCCAGAC AGCCAATGCC 
AAGGAAGACA GGCCACTCTT TTTCCTCACT GCAAGACACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_003754165.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003754213.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X3, mRNA.

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CloneID	ORa17144
Clone ID Related Accession (Same CDS sequence)	XM_003754165.2
Accession Version	XM_003754165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2013 this sequence version replaced XM_003754165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACTTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCCCC ATCAGAGGTG 
ACCTACCAGC AGACTGTGTT CCATGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAAC CTGTTACCAA GCAGAGGGCG GAGCAACACG TCCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAT 
TCTCAAAGTG ACCCATCTGT GGGATTAGTG GACAGACTGC TCAACTGCCG AGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC TCTCCATGTG ACCTCCTTCC CGTATGCGCT GCAGACTCAG 
CACACGCTCA TCAGCCCCTA CAATGAGATC CACTGGCCCA TCTCCTACAG CAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTT GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGGTAT GACAGCTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTGATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCAC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CAAAGCAGTG CCCCTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCTCCTC TGGCAGCTGT GGCCTATGAG 
CGACTGGCCC ATGTGCGGGC ACGGTTGGCT CTAGAAGAAC ACTTTGAGAT CATTCTGGGC 
CACCCCAGCT CCGGCATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACTTACTTGG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCGC TGCCACGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA TTCTTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAACTG 
GGTCTGGCTG CCTACTTTGT TAGCAACGAT ATCCCCTTGG AGAAGGGGCC CAAGAATGAG 
GCCTTGGAGA GTGACGGGGA GAAGCTGAGT AGCACAGACG AAGATGAGGA GGCAGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCCACCTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGT 
TCCCACTCCC ATGACTCCGC ATCTTCATCT CTCTCTTCCA AAGCGTCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGTTCA GCCCTCAGGA CTTCCACAGG GAGAGTTTGC CCGGTCATCT 
CCACCCTGTG GCCCTGCAGA GGAGGGCAGG GCCCCTGGGG AGATACAGCG GCTCCGAGTG 
AGTCAGGGAC CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGACGGAG GAGCCTGCAG GTGCCAGCCG CCCCATCCTC CCAGCTGTCC 
TCCTCCTCCT CGGGCGCATC ATCCACATGC ACGGTGTCCA CTGCCAATGT GCTAGTCCTT 
CAAGCTTCTC AGTGCTCCAT GGCCAAGGCC TGCCGGCAGC CACCCATTGT CTTCCTGCCT 
AAGCTCGTGT ATGACATGCT CCTGTCCACT GACAGCAGTG GCTTGCCCAA GTCTGCCTCC 
CTCCTGCCTT CCCCTTCGGT TATGTGGACC AGCTCCTTCC GCCCCCTCCT CAGCAAGATG 
ATGACGTCAA CTGAACAGTC CCTCTACTAC CGGCAGTGGA CAGTGCCCCG GCCCAGCCAC 
ATGGACTACG GCAACAGAGC TGAAGGCCGT GTGGACAGTT TCCACCCACG TAGGCTGCTG 
CTCAGTGGGC CACCTCAGAT TGGGAAGACG GGTGCCTACC TGCAGTTCCT CAGTATCTTG 
TCCAGGATGC TGATTCGGCT GACAGAAGTG GATGTTTACG ATGAAGAAGA GATCAATACC 
GGCCTCCAAG AGGAATCAGA TTGGCATTAC CTCCAGCTCA CCGACCCTTG GCCTGACCTG 
GAGCTCTTCC AGAAGATGCC TTTTGATTAC ATCATTCATG ACCCGAAGTA TGAGGATGCC 
AGTATGATTT GTTCACACCA CCAGAGCATC AAGAGTGAAG ACAGAGGGAT GTCCAGGAAG 
CCAGAGGACC TGTATGTGCG GCGTCAGACG GCACGTATGA GACTGTCTAA GTATGCGGCA 
TACAACACAT ACCACCATTG CGAGCAGTGC CAGCAGTACA TGGGCTTCCA CCCCCACTAC 
CAGCTCTCTG AGTCCACCCT GCACGTCTTT GCCTTCTCTT GCTCCATGCT AGGAGAGGAG 
ATCCAGCTCC ACTTCATCAT CCCCAAGTCC AAGGAGTACC ACTTTGTCTT CAGCCAACCG 
GGAGGCCAGC TGGAGAGCAT GCGCTTGCCC CTCGTCACAG ACAAGAGTTA TGAGCACATC 
AAAAGCCCGA CCTTCACCCC GACGACAGGC CGGCATGAGC ATGGGCTCTT TAATTTGTAC 
CATGCGATGG ATGGAGCCAA CCATCTACAT GTGCTGGTGG TCAAAGAGTA CGAAATGGCC 
ATTTATAAGA AGTACTGGCC CAACCACATC ATGCTTGTCC TCCCAAGCAT CTTTAACAGT 
GCTGGAGTCG GGGCTGCCCA CTTCCTGATC AAAGAGCTGT GCTACCACAA CCTGGAGCTG 
GAGAGGAACC GGCAGGAGGA GCTGGGGGTC AAGCCTCAGG ACATCTGGCC CTTCATCGTC 
ATCTCCGATG ACTCCTGTGT GATGTGGAAT GTTGCAGACG TGGACTGTGC TGGAGACCGG 
AGCAGGGAGT TTTCCTGGTC AGAGAGGAAT GTCTCTCTGA AACACATCAT GCAGCACATT 
GAGGCGTCCC CTAACATCAC ACACTACGCC CTGATCGGCA TGCGGAAGTG GGCCAGCAAG 
ACCCGGAGCC GTGAGGTGCA GGAGCCTTTT TCCCGATGCC ACGTGCATGA CTTCATCATC 
CTCAACGTAG ACTTGACCCA GAATGTACAG TACAACCAGA ATCGGTTTAC ATGTGACGAC 
GTAGACTTCA ACCTTCGCGT GCACAGTGCT GGCCTCCTGC TCTGCCGGTT CAATCGCTTC 
AGCGTCATGA AGAAGCAGAT CGCCGTGGGT GGGCACCGGT CCTTCCACAT CACATCCAAG 
GTGGCTGACA GCTCTGTGGC CATTGTGCCT TCCCAGTACA TCTGTGCCCC GGACAGTAAG 
CACACATTCC TGGCAGCTCC CGCCCAGCTC CTGCTGGAGA AGTTCCTCCA GTACCACAGC 
CACCGCTTCT TCCCGCTGTC TCTGAAGAAC CACAGCCACC CCGTGTTGTC TGTAGACTGC 
TACCTTAACC TGGGGCCTCA GATTTCTGTT TGTTATGTCA GCTCCAGGCC CCACTCCTTA 
AACGTCAGCT GCTCTGACAT GATGTTCAGC GGGCTCCTGC TGTACCTCTG CGACTCTTTT 
GTGGGGGCTA GCTTCTTGAA GAAGTTCCAT TTTCTGAAAG GAGCCACCTT GTGCGTGATC 
TGCCAAGACC GGAACTCGCT TCGACAGACG GTGGTCCGCC TGGAGCTAGA AGAGGAGTGG 
CAGTTCCGGC TGCGGGATGA GTTCCAGACA GCCAATGCCA AGGAAGACAG GCCACTCTTT 
TTCCTCACTG CAAGACACAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003754213.1
CDS	96..5879
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTNSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSKASGSVLYGESLVQPSGLPQGEFARSSPPCGPAEEGRAPGEIQRLRVSQGPTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSSGASSTCTVSTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTGLQEESDWHYLQLTDPWPDLELFQKMPFDYIIHDPKYEDASMICSHHQSIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEIQLHFIIPKSKEYHFVFSQPGGQLESMRLPLVTDKSYEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDIWPFIVISDDSCVMWNVADVDCAGDRSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWASKTRSREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVADSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNVSCSDMMFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_003754165.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003754165.2

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAATTCCAG CACCCAGAAT 
CTGGACTTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCCCC ATCAGAGGTG 
ACCTACCAGC AGACTGTGTT CCATGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAAC CTGTTACCAA GCAGAGGGCG GAGCAACACG TCCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAT 
TCTCAAAGTG ACCCATCTGT GGGATTAGTG GACAGACTGC TCAACTGCCG AGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC TCTCCATGTG ACCTCCTTCC CGTATGCGCT GCAGACTCAG 
CACACGCTCA TCAGCCCCTA CAATGAGATC CACTGGCCCA TCTCCTACAG CAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTT GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGGTAT GACAGCTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTGATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCAC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CAAAGCAGTG CCCCTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCTCCTC TGGCAGCTGT GGCCTATGAG 
CGACTGGCCC ATGTGCGGGC ACGGTTGGCT CTAGAAGAAC ACTTTGAGAT CATTCTGGGC 
CACCCCAGCT CCGGCATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACTTACTTGG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCGC TGCCACGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA TTCTTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAACTG 
GGTCTGGCTG CCTACTTTGT TAGCAACGAT ATCCCCTTGG AGAAGGGGCC CAAGAATGAG 
GCCTTGGAGA GTGACGGGGA GAAGCTGAGT AGCACAGACG AAGATGAGGA GGCAGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCCACCTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGT 
TCCCACTCCC ATGACTCCGC ATCTTCATCT CTCTCTTCCA AAGCGTCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGTTCA GCCCTCAGGA CTTCCACAGG GAGAGTTTGC CCGGTCATCT 
CCACCCTGTG GCCCTGCAGA GGAGGGCAGG GCCCCTGGGG AGATACAGCG GCTCCGAGTG 
AGTCAGGGAC CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGACGGAG GAGCCTGCAG GTGCCAGCCG CCCCATCCTC CCAGCTGTCC 
TCCTCCTCCT CGGGCGCATC ATCCACATGC ACGGTGTCCA CTGCCAATGT GCTAGTCCTT 
CAAGCTTCTC AGTGCTCCAT GGCCAAGGCC TGCCGGCAGC CACCCATTGT CTTCCTGCCT 
AAGCTCGTGT ATGACATGCT CCTGTCCACT GACAGCAGTG GCTTGCCCAA GTCTGCCTCC 
CTCCTGCCTT CCCCTTCGGT TATGTGGACC AGCTCCTTCC GCCCCCTCCT CAGCAAGATG 
ATGACGTCAA CTGAACAGTC CCTCTACTAC CGGCAGTGGA CAGTGCCCCG GCCCAGCCAC 
ATGGACTACG GCAACAGAGC TGAAGGCCGT GTGGACAGTT TCCACCCACG TAGGCTGCTG 
CTCAGTGGGC CACCTCAGAT TGGGAAGACG GGTGCCTACC TGCAGTTCCT CAGTATCTTG 
TCCAGGATGC TGATTCGGCT GACAGAAGTG GATGTTTACG ATGAAGAAGA GATCAATACC 
GGCCTCCAAG AGGAATCAGA TTGGCATTAC CTCCAGCTCA CCGACCCTTG GCCTGACCTG 
GAGCTCTTCC AGAAGATGCC TTTTGATTAC ATCATTCATG ACCCGAAGTA TGAGGATGCC 
AGTATGATTT GTTCACACCA CCAGAGCATC AAGAGTGAAG ACAGAGGGAT GTCCAGGAAG 
CCAGAGGACC TGTATGTGCG GCGTCAGACG GCACGTATGA GACTGTCTAA GTATGCGGCA 
TACAACACAT ACCACCATTG CGAGCAGTGC CAGCAGTACA TGGGCTTCCA CCCCCACTAC 
CAGCTCTCTG AGTCCACCCT GCACGTCTTT GCCTTCTCTT GCTCCATGCT AGGAGAGGAG 
ATCCAGCTCC ACTTCATCAT CCCCAAGTCC AAGGAGTACC ACTTTGTCTT CAGCCAACCG 
GGAGGCCAGC TGGAGAGCAT GCGCTTGCCC CTCGTCACAG ACAAGAGTTA TGAGCACATC 
AAAAGCCCGA CCTTCACCCC GACGACAGGC CGGCATGAGC ATGGGCTCTT TAATTTGTAC 
CATGCGATGG ATGGAGCCAA CCATCTACAT GTGCTGGTGG TCAAAGAGTA CGAAATGGCC 
ATTTATAAGA AGTACTGGCC CAACCACATC ATGCTTGTCC TCCCAAGCAT CTTTAACAGT 
GCTGGAGTCG GGGCTGCCCA CTTCCTGATC AAAGAGCTGT GCTACCACAA CCTGGAGCTG 
GAGAGGAACC GGCAGGAGGA GCTGGGGGTC AAGCCTCAGG ACATCTGGCC CTTCATCGTC 
ATCTCCGATG ACTCCTGTGT GATGTGGAAT GTTGCAGACG TGGACTGTGC TGGAGACCGG 
AGCAGGGAGT TTTCCTGGTC AGAGAGGAAT GTCTCTCTGA AACACATCAT GCAGCACATT 
GAGGCGTCCC CTAACATCAC ACACTACGCC CTGATCGGCA TGCGGAAGTG GGCCAGCAAG 
ACCCGGAGCC GTGAGGTGCA GGAGCCTTTT TCCCGATGCC ACGTGCATGA CTTCATCATC 
CTCAACGTAG ACTTGACCCA GAATGTACAG TACAACCAGA ATCGGTTTAC ATGTGACGAC 
GTAGACTTCA ACCTTCGCGT GCACAGTGCT GGCCTCCTGC TCTGCCGGTT CAATCGCTTC 
AGCGTCATGA AGAAGCAGAT CGCCGTGGGT GGGCACCGGT CCTTCCACAT CACATCCAAG 
GTGGCTGACA GCTCTGTGGC CATTGTGCCT TCCCAGTACA TCTGTGCCCC GGACAGTAAG 
CACACATTCC TGGCAGCTCC CGCCCAGCTC CTGCTGGAGA AGTTCCTCCA GTACCACAGC 
CACCGCTTCT TCCCGCTGTC TCTGAAGAAC CACAGCCACC CCGTGTTGTC TGTAGACTGC 
TACCTTAACC TGGGGCCTCA GATTTCTGTT TGTTATGTCA GCTCCAGGCC CCACTCCTTA 
AACGTCAGCT GCTCTGACAT GATGTTCAGC GGGCTCCTGC TGTACCTCTG CGACTCTTTT 
GTGGGGGCTA GCTTCTTGAA GAAGTTCCAT TTTCTGAAAG GAGCCACCTT GTGCGTGATC 
TGCCAAGACC GGAACTCGCT TCGACAGACG GTGGTCCGCC TGGAGCTAGA AGAGGAGTGG 
CAGTTCCGGC TGCGGGATGA GTTCCAGACA GCCAATGCCA AGGAAGACAG GCCACTCTTT 
TTCCTCACTG CAAGACACAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_006225732.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006225794.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa17145
Clone ID Related Accession (Same CDS sequence)	XM_006225732.2
Accession Version	XM_006225732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006225732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCACAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCAAAGC AGTGCCCCTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCT 
CCTCTGGCAG CTGTGGCCTA TGAGCGACTG GCCCATGTGC GGGCACGGTT GGCTCTAGAA 
GAACACTTTG AGATCATTCT GGGCCACCCC AGCTCCGGCA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACTTAC 
TTGGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCGCTGCCAC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAATTCTTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA ACTGGGTCTG GCTGCCTACT TTGTTAGCAA CGATATCCCC 
TTGGAGAAGG GGCCCAAGAA TGAGGCCTTG GAGAGTGACG GGGAGAAGCT GAGTAGCACA 
GACGAAGATG AGGAGGCAGG GACAGAAGGG TGTTTTGAAG CAGGCTCCAC CTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGTTCCCAC TCCCATGACT CCGCATCTTC ATCTCTCTCT 
TCCAAAGCGT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG TTCAGCCCTC AGGACTTCCA 
CAGGGAGAGT TTGCCCGGTC ATCTCCACCC TGTGGCCCTG CAGAGGAGGG CAGGGCCCCT 
GGGGAGATAC AGCGGCTCCG AGTGAGTCAG GGACCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGAC GGAGGAGCCT GCAGGTGCCA 
GCCGCCCCAT CCTCCCAGCT GTCCTCCTCC TCCTCGGGCG CATCATCCAC ATGCACGGTG 
TCCACTGCCA ATGTGCTAGT CCTTCAAGCT TCTCAGTGCT CCATGGCCAA GGCCTGCCGG 
CAGCCACCCA TTGTCTTCCT GCCTAAGCTC GTGTATGACA TGCTCCTGTC CACTGACAGC 
AGTGGCTTGC CCAAGTCTGC CTCCCTCCTG CCTTCCCCTT CGGTTATGTG GACCAGCTCC 
TTCCGCCCCC TCCTCAGCAA GATGATGACG TCAACTGAAC AGTCCCTCTA CTACCGGCAG 
TGGACAGTGC CCCGGCCCAG CCACATGGAC TACGGCAACA GAGCTGAAGG CCGTGTGGAC 
AGTTTCCACC CACGTAGGCT GCTGCTCAGT GGGCCACCTC AGATTGGGAA GACGGGTGCC 
TACCTGCAGT TCCTCAGTAT CTTGTCCAGG ATGCTGATTC GGCTGACAGA AGTGGATGTT 
TACGATGAAG AAGAGATCAA TACCGGCCTC CAAGAGGAAT CAGATTGGCA TTACCTCCAG 
CTCACCGACC CTTGGCCTGA CCTGGAGCTC TTCCAGAAGA TGCCTTTTGA TTACATCATT 
CATGACCCGA AGTATGAGGA TGCCAGTATG ATTTGTTCAC ACCACCAGAG CATCAAGAGT 
GAAGACAGAG GGATGTCCAG GAAGCCAGAG GACCTGTATG TGCGGCGTCA GACGGCACGT 
ATGAGACTGT CTAAGTATGC GGCATACAAC ACATACCACC ATTGCGAGCA GTGCCAGCAG 
TACATGGGCT TCCACCCCCA CTACCAGGAG AGGAGATCCA GCTCCACTTC ATCATCCCCA 
AGTCCAAGGA GTACCACTTT GTCTTCAGCC AACCGGGAGG CCAGCTGGAG AGCATGCGCT 
TGCCCCTCGT CACAGACAAG AGTTATGAGC ACATCAAAAG CCCGACCTTC ACCCCGACGA 
CAGGCCGGCA TGAGCATGGG CTCTTTAATT TGTACCATGC GATGGATGGA GCCAACCATC 
TACATGTGCT GGTGGTCAAA GAGTACGAAA TGGCCATTTA TAAGAAGTAC TGGCCCAACC 
ACATCATGCT TGTCCTCCCA AGCATCTTTA ACAGTGCTGG AGTCGGGGCT GCCCACTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006225794.1
CDS	96..4898
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSKASGSVLYGESLVQPSGLPQGEFARSSPPCGPAEEGRAPGEIQRLRVSQGPTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSSGASSTCTVSTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTGLQEESDWHYLQLTDPWPDLELFQKMPFDYIIHDPKYEDASMICSHHQSIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQERRSSSTSSSPSPRSTTLSSANREASWRACACPSSQTRVMSTSKARPSPRRQAGMSMGSLICTMRWMEPTIYMCWWSKSTKWPFIRSTGPTTSCLSSQASLTVLESGLPTS

Target ORF information:

RefSeq Version	XM_006225732.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006225732.2

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCACAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCAAAGC AGTGCCCCTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCT 
CCTCTGGCAG CTGTGGCCTA TGAGCGACTG GCCCATGTGC GGGCACGGTT GGCTCTAGAA 
GAACACTTTG AGATCATTCT GGGCCACCCC AGCTCCGGCA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACTTAC 
TTGGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCGCTGCCAC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAATTCTTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA ACTGGGTCTG GCTGCCTACT TTGTTAGCAA CGATATCCCC 
TTGGAGAAGG GGCCCAAGAA TGAGGCCTTG GAGAGTGACG GGGAGAAGCT GAGTAGCACA 
GACGAAGATG AGGAGGCAGG GACAGAAGGG TGTTTTGAAG CAGGCTCCAC CTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGTTCCCAC TCCCATGACT CCGCATCTTC ATCTCTCTCT 
TCCAAAGCGT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG TTCAGCCCTC AGGACTTCCA 
CAGGGAGAGT TTGCCCGGTC ATCTCCACCC TGTGGCCCTG CAGAGGAGGG CAGGGCCCCT 
GGGGAGATAC AGCGGCTCCG AGTGAGTCAG GGACCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGAC GGAGGAGCCT GCAGGTGCCA 
GCCGCCCCAT CCTCCCAGCT GTCCTCCTCC TCCTCGGGCG CATCATCCAC ATGCACGGTG 
TCCACTGCCA ATGTGCTAGT CCTTCAAGCT TCTCAGTGCT CCATGGCCAA GGCCTGCCGG 
CAGCCACCCA TTGTCTTCCT GCCTAAGCTC GTGTATGACA TGCTCCTGTC CACTGACAGC 
AGTGGCTTGC CCAAGTCTGC CTCCCTCCTG CCTTCCCCTT CGGTTATGTG GACCAGCTCC 
TTCCGCCCCC TCCTCAGCAA GATGATGACG TCAACTGAAC AGTCCCTCTA CTACCGGCAG 
TGGACAGTGC CCCGGCCCAG CCACATGGAC TACGGCAACA GAGCTGAAGG CCGTGTGGAC 
AGTTTCCACC CACGTAGGCT GCTGCTCAGT GGGCCACCTC AGATTGGGAA GACGGGTGCC 
TACCTGCAGT TCCTCAGTAT CTTGTCCAGG ATGCTGATTC GGCTGACAGA AGTGGATGTT 
TACGATGAAG AAGAGATCAA TACCGGCCTC CAAGAGGAAT CAGATTGGCA TTACCTCCAG 
CTCACCGACC CTTGGCCTGA CCTGGAGCTC TTCCAGAAGA TGCCTTTTGA TTACATCATT 
CATGACCCGA AGTATGAGGA TGCCAGTATG ATTTGTTCAC ACCACCAGAG CATCAAGAGT 
GAAGACAGAG GGATGTCCAG GAAGCCAGAG GACCTGTATG TGCGGCGTCA GACGGCACGT 
ATGAGACTGT CTAAGTATGC GGCATACAAC ACATACCACC ATTGCGAGCA GTGCCAGCAG 
TACATGGGCT TCCACCCCCA CTACCAGGAG AGGAGATCCA GCTCCACTTC ATCATCCCCA 
AGTCCAAGGA GTACCACTTT GTCTTCAGCC AACCGGGAGG CCAGCTGGAG AGCATGCGCT 
TGCCCCTCGT CACAGACAAG AGTTATGAGC ACATCAAAAG CCCGACCTTC ACCCCGACGA 
CAGGCCGGCA TGAGCATGGG CTCTTTAATT TGTACCATGC GATGGATGGA GCCAACCATC 
TACATGTGCT GGTGGTCAAA GAGTACGAAA TGGCCATTTA TAAGAAGTAC TGGCCCAACC 
ACATCATGCT TGTCCTCCCA AGCATCTTTA ACAGTGCTGG AGTCGGGGCT GCCCACTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_008776221.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008774443.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X4, mRNA.

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CloneID	ORa49405
Clone ID Related Accession (Same CDS sequence)	XM_008776221.2
Accession Version	XM_008776221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008776221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCACAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCAAAGC AGTGCCCCTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCT 
CCTCTGGCAG CTGTGGCCTA TGAGCGACTG GCCCATGTGC GGGCACGGTT GGCTCTAGAA 
GAACACTTTG AGATCATTCT GGGCCACCCC AGCTCCGGCA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACTTAC 
TTGGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCGCTGCCAC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAATTCTTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA ACTGGGTCTG GCTGCCTACT TTGTTAGCAA CGATATCCCC 
TTGGAGAAGG GGCCCAAGAA TGAGGCCTTG GAGAGTGACG GGGAGAAGCT GAGTAGCACA 
GACGAAGATG AGGAGGCAGG GACAGAAGGG TGTTTTGAAG CAGGCTCCAC CTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGTTCCCAC TCCCATGACT CCGCATCTTC ATCTCTCTCT 
TCCAAAGCGT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG TTCAGCCCTC AGGACTTCCA 
CAGGGAGAGT TTGCCCGGTC ATCTCCACCC TGTGGCCCTG CAGAGGAGGG CAGGGCCCCT 
GGGGAGATAC AGCGGCTCCG AGTGAGTCAG GGACCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGAC GGAGGAGCCT GCAGGTGCCA 
GCCGCCCCAT CCTCCCAGCT GTCCTCCTCC TCCTCGGGCG CATCATCCAC ATGCACGGTG 
TCCACTGCCA ATGTGCTAGT CCTTCAAGCT TCTCAGTGCT CCATGGCCAA GGCCTGCCGG 
CAGCCACCCA TTGTCTTCCT GCCTAAGCTC GTGTATGACA TGCTCCTGTC CACTGACAGC 
AGTGGCTTGC CCAAGTCTGC CTCCCTCCTG CCTTCCCCTT CGGTTATGTG GACCAGCTCC 
TTCCGCCCCC TCCTCAGCAA GATGATGACG TCAACTGAAC AGTCCCTCTA CTACCGGCAG 
TGGACAGTGC CCCGGCCCAG CCACATGGAC TACGGCAACA GAGCTGAAGG CCGTGTGGAC 
AGTTTCCACC CACGTAGGCT GCTGCTCAGT GGGCCACCTC AGATTGGGAA GACGGGTGCC 
TACCTGCAGT TCCTCAGTAT CTTGTCCAGG ATGCTGATTC GGCTGACAGA AGTGGATGTT 
TACGATGAAG AAGAGATCAA TACCGGCCTC CAAGAGGAAT CAGATTGGCA TTACCTCCAG 
CTCACCGACC CTTGGCCTGA CCTGGAGCTC TTCCAGAAGA TGCCTTTTGA TTACATCATT 
CATGACCCGA AGTATGAGGA TGCCAGTATG ATTTGTTCAC ACCACCAGAG CATCAAGAGT 
GAAGACAGAG GGATGTCCAG GAAGCCAGAG GACCTGTATG TGCGGCGTCA GACGGCACGT 
ATGAGACTGT CTAAGTATGC GGCATACAAC ACATACCACC ATTGCGAGCA GTGCCAGCAG 
TACATGGGCT TCCACCCCCA CTACCAGCTC TCTGAGTCCA CCCTGCACGT CTTTGCCTTC 
TCTTGCTCCA TGCTAGGAGA GGAGATCCAG CTCCACTTCA TCATCCCCAA GTCCAAGGAG 
TACCACTTTG TCTTCAGCCA ACCGGGAGGC CAGCTGGAGA GCATGCGCTT GCCCCTCGTC 
ACAGACAAGA GTTATGAGCA CATCAAAAGC CCGACCTTCA CCCCGACGAC AGGCCGGCAT 
GAGCATGGGC TCTTTAATTT GTACCATGCG ATGGATGGAG CCAACCATCT ACATGTGCTG 
GTGGTCAAAG AGTACGAAAT GGCCATTTAT AAGAAGTACT GGCCCAACCA CATCATGCTT 
GTCCTCCCAA GCATCTTTAA CAGTGCTGGA GTCGGGGCTG CCCACTTCCT GATCAAAGAG 
CTGTGCTACC ACAACCTGGA GCTGGAGAGG AACCGGCAGG AGGAGCTGGG GGTCAAGCCT 
CAGGACATCT GGCCCTTCAT CGTCATCTCC GATGACTCCT GTGTGATGTG GAATGTTGCA 
GACGTGGACT GTGCTGGAGA CCGGAGCAGG GAGTTTTCCT GGTCAGAGAG GAATGTCTCT 
CTGAAACACA TCATGCAGCA CATTGAGGCG TCCCCTAACA TCACACACTA CGCCCTGATC 
GGCATGCGGA AGTGGGCCAG CAAGACCCGG AGCCGTGAGG TGCAGGAGCC TTTTTCCCGA 
TGCCACGTGC ATGACTTCAT CATCCTCAAC GTAGACTTGA CCCAGAATGT ACAGTACAAC 
CAGAATCGGT TTACATGTGA CGACGTAGAC TTCAACCTTC GCGTGCACAG TGCTGGCCTC 
CTGCTCTGCC GGTTCAATCG CTTCAGCGTC ATGAAGAAGC AGATCGCCGT GGGTGGGCAC 
CGGTCCTTCC ACATCACATC CAAGGTATGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008774443.1
CDS	96..5471
Translation	MGNSYAGQLKSTRFEEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSKASGSVLYGESLVQPSGLPQGEFARSSPPCGPAEEGRAPGEIQRLRVSQGPTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSSGASSTCTVSTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTGLQEESDWHYLQLTDPWPDLELFQKMPFDYIIHDPKYEDASMICSHHQSIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEIQLHFIIPKSKEYHFVFSQPGGQLESMRLPLVTDKSYEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDIWPFIVISDDSCVMWNVADVDCAGDRSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWASKTRSREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVCS

Target ORF information:

RefSeq Version	XM_008776221.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008776221.2

ORF Insert Sequence:

ATGGGCAATT CTTACGCTGG ACAGCTAAAG AGCACGCGGT TTGAAGAGGT CCTCCACAAC 
TCCATCGAGG CTTCCCTACG CTCCAACACC CTGGTGCCCC GGCCCATCTT CTCCCAGCTG 
TATCTGGAGG CCGAGCAGCA GTTGTCATCA TTGGAAGGCG GCAGCCGAGC GGACAATGAG 
GAAGAAGAAG AAGATGGGGA AGGAGGGCTA GATCCCAGCA GTCCCCCAAA TGCCTACCAG 
CTGCCCCCTC CTCCTGAAGG ATGTTGCACC ACAGATGGCT TCTGTCAGGC TGGGAAGGAC 
CTACGTCTTG TCTCCATTTC CAATGAGCCC ATTGAAGTGC CTGCAGGCTT CCTCCTGGTG 
GGTGCCAAGT CTCCCAGCCT GCCAGACCAC CTCCTGGTGT GTGCAGTGGA CAAGAGGTTC 
TTGCCCGATG ACAATGGCCA CAATGCCCTG CTCGGTTTCT CTGGGAACTG TGTTGGCTGT 
GGGAAAAAAG GCTTCTGCTA TTTCACCGAA TTCTCCAACC ACATCAACCT GAAGCTGACC 
ACGCAGCCCA AGAAACAGAA GCACCTCAAG TACTATCTGG TTCGAAATGC ACAAGGGGCA 
CTGACCAAAG GACCTTTGAT TTGCTGGAAA GGCTCTGAGT TCAGAAGTCG GCAGAACTCC 
ACCAACACCT GCTCCAGTTC TCTGTTCCCA CCACTGGAGA GTTCAGGGTC CCTGGCCGCC 
TTCCCCACTG AGCCTGCTCC TGGGACAAAC CCCAGTGTCC CAGTGGGAGC TCAACAGGCA 
GGACCAGCCT CTGATCACCC CTCAGTGGCC ACAGCAACAG GTCCTGCTGT CTTCAATGGC 
AAGGATTCCC CCAAGCATCC ACAGCTGGTG AAGAATAGCC TGTGTGCCGC GCCGCGCCCC 
ACAGCCTTAG GTCTCTTGCC AAACTCTGGG CCTCCCAAAA AACGGCACAA AGGGTGGTCC 
CCAGAGTCTA AATCAACCAC AGATGGCAGC TTCATCCAGG CTGTCGGCAA CAGAGCTAAA 
CATGAAGGTG CCAGCATGCC TTGTGTACCC CAGGCTGGCT TGGTGGGACC AACTTCAGTC 
ACCTTCCCGG TGGTGGCCTC CAGCGAACCA GTGTCAGTTC CGGATAACTT GCTGAAAATA 
TGCAAGGCCA AGCCAGTGAT ATTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGAAAC 
CTGAATGATG TGGTCGTCAG CCCCCTACTG TACACCTGCT ACCAGAACTC GCAGTCACTT 
TCCAGGTCCT ATGAGCAACA CGGTGCCTCC ACCATGCAGC CCATATCTGA AGAGACACAG 
CTGTTGCTGA CCGTCTACTA CCTCGTCCAG CTGGCTGCAG ACCAGGTGCC ACTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCCTGG CGAGAGTCAC ATCTGACGGA GATAAGGCAG 
TACCAGCAGG TGCCTCCACA GCCCTTCCCG CCAGCCACCA GCACAGCAGC GCCTGTGACT 
TCTGCACAGC TGCCCTGGCT GGCAGGCCTG GCCGCCAGCT CCTGCAATGA CAGCGTGCAT 
GTTATCGAAT GCGCTTACTC GCTGGCTGAG GGCCTCTCCG AGATGTTCCG GTTGCTCATT 
GAGGGTAAAC TCTCCAAGAC CAACTATGTG GTCATCATCT GTGCCTGCCG AAATGCCGCC 
ATTGACTCCT GCATCGCTGT CACCGGGAAA TACCAAGCCC GGATTCTCTC CGAGAGCCTC 
CTCAGTCCTG CAGAGTACCA GAGAGAAGTC CACTATGAGC TGGTCACTGG CAAAGTGGAC 
TCGCTGGGGG CCTTCTTTAG CAGCCTCTGT CCAGAGGGTG ACATTGACAT TTTGCTGGAC 
AAATTTCACC AGGAAAATCA AGGCCATGTC TCTTCCTCAT TCACTGCTTC CTCTACCAAG 
AAAACAGCCG TGCTGGATGC CAGTGGGGTT CCCATGTGCA CAAGTTACCA TCAGGAGCCA 
CGGGGCATCC GGCCCTTTCA GCTGGCGGTG GCGCAGAAGC TCCTTTCCCA CGTGTGCTCA 
ATCGCAGATT CCAGCACCCA GAATCTGGAC TTGGGGTCCT TCGAGAAGGT GGACTTTCTC 
ATTTGCATTC CCCCATCAGA GGTGACCTAC CAGCAGACTG TGTTCCATGT CTGGCATTCA 
GGGGTTCTGC TGGAGCTGGG CCTGGAGAAA GAACCTGTTA CCAAGCAGAG GGCGGAGCAA 
CACGTCCTGA AACTAGACAC GGAGGCCCAG GCAAGATTTA AGGCCTTTCT GCAAAACTCC 
TTCCAGAACC CACATACGCT CTTTGTCCTC ATCCACGACC ACGCCCACTG GGATCTAGTG 
AGCAGTGCTG TTCATAACAT CTATTCTCAA AGTGACCCAT CTGTGGGATT AGTGGACAGA 
CTGCTCAACT GCCGAGAGGT GAAAGAGGCG CCCAACATTG TGACTCTCCA TGTGACCTCC 
TTCCCGTATG CGCTGCAGAC TCAGCACACG CTCATCAGCC CCTACAATGA GATCCACTGG 
CCCATCTCCT ACAGCAATGG AGTGGACCTG TATCATGAGA GTAAGAAGTA CTTTGGGCTG 
TCTGAGTTTA TTGACTCCAC CCTGTCAGGA CACAGTCTTC CTCTGCTCCG GTATGACAGC 
TCCTTTGAGG CCATGGTCAC TGCATTGGGG AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTTGT GCAGCACTAT GCGGCCGCCA TGATGGCCGT GAGTGGCCTC 
CCACAGATGA AGAACCATAC ATCAGTGGAG ACCCTAGAGA TCACCCAGAA CCTCCTCAAC 
TCCCCAAAGC AGTGCCCCTG TGGCCACGGG CTCATGGTCC TGCTGCGCGT GCCCTGTTCT 
CCTCTGGCAG CTGTGGCCTA TGAGCGACTG GCCCATGTGC GGGCACGGTT GGCTCTAGAA 
GAACACTTTG AGATCATTCT GGGCCACCCC AGCTCCGGCA TCACTGTAGG CAAGCACTTT 
GTGAAGCAGC TCAAGATGTG GCAGAAAATT GAGGATGCAG AATGGAGGCC TCAGACTTAC 
TTGGAGTTGG AGGGCCTGCC CTGCATCCTG ATCTTCAGTG GGATGGACCC TCATGGGGAG 
TCGCTGCCAC GGTCTCTGAG GTACTGTGAC CTGCGTCTAA TAAATTCTTC CTGCCTGGTG 
AGGACAGCCC TGGAGCAGGA ACTGGGTCTG GCTGCCTACT TTGTTAGCAA CGATATCCCC 
TTGGAGAAGG GGCCCAAGAA TGAGGCCTTG GAGAGTGACG GGGAGAAGCT GAGTAGCACA 
GACGAAGATG AGGAGGCAGG GACAGAAGGG TGTTTTGAAG CAGGCTCCAC CTCAGAGCAG 
AGGGGCCCTG TGAAGAGGGA ACGTTCCCAC TCCCATGACT CCGCATCTTC ATCTCTCTCT 
TCCAAAGCGT CTGGCTCGGT GCTCTATGGT GAGTCCCTGG TTCAGCCCTC AGGACTTCCA 
CAGGGAGAGT TTGCCCGGTC ATCTCCACCC TGTGGCCCTG CAGAGGAGGG CAGGGCCCCT 
GGGGAGATAC AGCGGCTCCG AGTGAGTCAG GGACCTACAG TCATCAGTAG GCACAGCCCT 
GGCCTGGTCC CACAGCCTGA TTCTAGCCTC AGGACTGGAC GGAGGAGCCT GCAGGTGCCA 
GCCGCCCCAT CCTCCCAGCT GTCCTCCTCC TCCTCGGGCG CATCATCCAC ATGCACGGTG 
TCCACTGCCA ATGTGCTAGT CCTTCAAGCT TCTCAGTGCT CCATGGCCAA GGCCTGCCGG 
CAGCCACCCA TTGTCTTCCT GCCTAAGCTC GTGTATGACA TGCTCCTGTC CACTGACAGC 
AGTGGCTTGC CCAAGTCTGC CTCCCTCCTG CCTTCCCCTT CGGTTATGTG GACCAGCTCC 
TTCCGCCCCC TCCTCAGCAA GATGATGACG TCAACTGAAC AGTCCCTCTA CTACCGGCAG 
TGGACAGTGC CCCGGCCCAG CCACATGGAC TACGGCAACA GAGCTGAAGG CCGTGTGGAC 
AGTTTCCACC CACGTAGGCT GCTGCTCAGT GGGCCACCTC AGATTGGGAA GACGGGTGCC 
TACCTGCAGT TCCTCAGTAT CTTGTCCAGG ATGCTGATTC GGCTGACAGA AGTGGATGTT 
TACGATGAAG AAGAGATCAA TACCGGCCTC CAAGAGGAAT CAGATTGGCA TTACCTCCAG 
CTCACCGACC CTTGGCCTGA CCTGGAGCTC TTCCAGAAGA TGCCTTTTGA TTACATCATT 
CATGACCCGA AGTATGAGGA TGCCAGTATG ATTTGTTCAC ACCACCAGAG CATCAAGAGT 
GAAGACAGAG GGATGTCCAG GAAGCCAGAG GACCTGTATG TGCGGCGTCA GACGGCACGT 
ATGAGACTGT CTAAGTATGC GGCATACAAC ACATACCACC ATTGCGAGCA GTGCCAGCAG 
TACATGGGCT TCCACCCCCA CTACCAGCTC TCTGAGTCCA CCCTGCACGT CTTTGCCTTC 
TCTTGCTCCA TGCTAGGAGA GGAGATCCAG CTCCACTTCA TCATCCCCAA GTCCAAGGAG 
TACCACTTTG TCTTCAGCCA ACCGGGAGGC CAGCTGGAGA GCATGCGCTT GCCCCTCGTC 
ACAGACAAGA GTTATGAGCA CATCAAAAGC CCGACCTTCA CCCCGACGAC AGGCCGGCAT 
GAGCATGGGC TCTTTAATTT GTACCATGCG ATGGATGGAG CCAACCATCT ACATGTGCTG 
GTGGTCAAAG AGTACGAAAT GGCCATTTAT AAGAAGTACT GGCCCAACCA CATCATGCTT 
GTCCTCCCAA GCATCTTTAA CAGTGCTGGA GTCGGGGCTG CCCACTTCCT GATCAAAGAG 
CTGTGCTACC ACAACCTGGA GCTGGAGAGG AACCGGCAGG AGGAGCTGGG GGTCAAGCCT 
CAGGACATCT GGCCCTTCAT CGTCATCTCC GATGACTCCT GTGTGATGTG GAATGTTGCA 
GACGTGGACT GTGCTGGAGA CCGGAGCAGG GAGTTTTCCT GGTCAGAGAG GAATGTCTCT 
CTGAAACACA TCATGCAGCA CATTGAGGCG TCCCCTAACA TCACACACTA CGCCCTGATC 
GGCATGCGGA AGTGGGCCAG CAAGACCCGG AGCCGTGAGG TGCAGGAGCC TTTTTCCCGA 
TGCCACGTGC ATGACTTCAT CATCCTCAAC GTAGACTTGA CCCAGAATGT ACAGTACAAC 
CAGAATCGGT TTACATGTGA CGACGTAGAC TTCAACCTTC GCGTGCACAG TGCTGGCCTC 
CTGCTCTGCC GGTTCAATCG CTTCAGCGTC ATGAAGAAGC AGATCGCCGT GGGTGGGCAC 
CGGTCCTTCC ACATCACATC CAAGGTATGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Greb1 ( XM_008764638.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762860.1 Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X1, mRNA.

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CloneID	ORa49410
Clone ID Related Accession (Same CDS sequence)	XM_008764638.1
Accession Version	XM_008764638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCAGAACA ATCCCAGAGA AGAGGTCCTC CACAACTCCA TCGAGGCTTC CCTACGCTCC 
AACACCCTGG TGCCCCGGCC CATCTTCTCC CAGCTGTATC TGGAGGCCGA GCAGCAGTTG 
TCATCATTGG AAGGCGGCAG CCGAGCGGAC AATGAGGAAG AAGAAGAAGA TGGGGAAGGA 
GGGCTAGATC CCAGCAGTCC CCCAAATGCC TACCAGCTGC CCCCTCCTCC TGAAGGATGT 
TGCACCACAG ATGGCTTCTG TCAGGCTGGG AAGGACCTAC GTCTTGTCTC CATTTCCAAT 
GAGCCCATTG AAGTGCCTGC AGGCTTCCTC CTGGTGGGTG CCAAGTCTCC CAGCCTGCCA 
GACCACCTCC TGGTGTGTGC AGTGGACAAG AGGTTCTTGC CCGATGACAA TGGCCACAAT 
GCCCTGCTCG GTTTCTCTGG GAACTGTGTT GGCTGTGGGA AAAAAGGCTT CTGCTATTTC 
ACCGAATTCT CCAACCACAT CAACCTGAAG CTGACCACGC AGCCCAAGAA ACAGAAGCAC 
CTCAAGTACT ATCTGGTTCG AAATGCACAA GGGGCACTGA CCAAAGGACC TTTGATTTGC 
TGGAAAGGCT CTGAGTTCAG AAGTCGGCAG AACTCCACCA ACACCTGCTC CAGTTCTCTG 
TTCCCACCAC TGGAGAGTTC AGGGTCCCTG GCCGCCTTCC CCACTGAGCC TGCTCCTGGG 
ACAAACCCCA GTGTCCCAGT GGGAGCTCAA CAGGCAGGAC CAGCCTCTGA TCACCCCTCA 
GTGGCCACAG CAACAGGTCC TGCTGTCTTC AATGGCAAGG ATTCCCCCAA GCATCCACAG 
CTGGTGAAGA ATAGCCTGTG TGCCGCGCCG CGCCCCACAG CCTTAGGTCT CTTGCCAAAC 
TCTGGGCCTC CCAAAAAACG GCACAAAGGG TGGTCCCCAG AGTCTAAATC AACCACAGAT 
GGCAGCTTCA TCCAGGCTGT CGGCAACAGA GCTAAACATG AAGGTGCCAG CATGCCTTGT 
GTACCCCAGG CTGGCTTGGT GGGACCAACT TCAGTCACCT TCCCGGTGGT GGCCTCCAGC 
GAACCAGTGT CAGTTCCGGA TAACTTGCTG AAAATATGCA AGGCCAAGCC AGTGATATTT 
AAAGGCCACG GGAACTTCCC CTACCTCTGC GGAAACCTGA ATGATGTGGT CGTCAGCCCC 
CTACTGTACA CCTGCTACCA GAACTCGCAG TCACTTTCCA GGTCCTATGA GCAACACGGT 
GCCTCCACCA TGCAGCCCAT ATCTGAAGAG ACACAGCTGT TGCTGACCGT CTACTACCTC 
GTCCAGCTGG CTGCAGACCA GGTGCCACTG ATGGAGGACC TGGAGCAGAT CTTCCTGCGC 
TCCTGGCGAG AGTCACATCT GACGGAGATA AGGCAGTACC AGCAGGTGCC TCCACAGCCC 
TTCCCGCCAG CCACCAGCAC AGCAGCGCCT GTGACTTCTG CACAGCTGCC CTGGCTGGCA 
GGCCTGGCCG CCAGCTCCTG CAATGACAGC GTGCATGTTA TCGAATGCGC TTACTCGCTG 
GCTGAGGGCC TCTCCGAGAT GTTCCGGTTG CTCATTGAGG GTAAACTCTC CAAGACCAAC 
TATGTGGTCA TCATCTGTGC CTGCCGAAAT GCCGCCATTG ACTCCTGCAT CGCTGTCACC 
GGGAAATACC AAGCCCGGAT TCTCTCCGAG AGCCTCCTCA GTCCTGCAGA GTACCAGAGA 
GAAGTCCACT ATGAGCTGGT CACTGGCAAA GTGGACTCGC TGGGGGCCTT CTTTAGCAGC 
CTCTGTCCAG AGGGTGACAT TGACATTTTG CTGGACAAAT TTCACCAGGA AAATCAAGGC 
CATGTCTCTT CCTCATTCAC TGCTTCCTCT ACCAAGAAAA CAGCCGTGCT GGATGCCAGT 
GGGGTTCCCA TGTGCACAAG TTACCATCAG GAGCCACGGG GCATCCGGCC CTTTCAGCTG 
GCGGTGGCGC AGAAGCTCCT TTCCCACGTG TGCTCAATCG CAGATTCCAG CACCCAGAAT 
CTGGACTTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCCCC ATCAGAGGTG 
ACCTACCAGC AGACTGTGTT CCATGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAAC CTGTTACCAA GCAGAGGGCG GAGCAACACG TCCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAT 
TCTCAAAGTG ACCCATCTGT GGGATTAGTG GACAGACTGC TCAACTGCCG AGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC TCTCCATGTG ACCTCCTTCC CGTATGCGCT GCAGACTCAG 
CACACGCTCA TCAGCCCCTA CAATGAGATC CACTGGCCCA TCTCCTACAG CAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTT GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGGTAT GACAGCTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTGATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCAC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CAAAGCAGTG CCCCTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCTCCTC TGGCAGCTGT GGCCTATGAG 
CGACTGGCCC ATGTGCGGGC ACGGTTGGCT CTAGAAGAAC ACTTTGAGAT CATTCTGGGC 
CACCCCAGCT CCGGCATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACTTACTTGG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCGC TGCCACGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA TTCTTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAACTG 
GGTCTGGCTG CCTACTTTGT TAGCAACGAT ATCCCCTTGG AGAAGGGGCC CAAGAATGAG 
GCCTTGGAGA GTGACGGGGA GAAGCTGAGT AGCACAGACG AAGATGAGGA GGCAGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCCACCTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGT 
TCCCACTCCC ATGACTCCGC ATCTTCATCT CTCTCTTCCA AAGCGTCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGTTCA GCCCTCAGGA CTTCCACAGG GAGAGTTTGC CCGGTCATCT 
CCACCCTGTG GCCCTGCAGA GGAGGGCAGG GCCCCTGGGG AGATACAGCG GCTCCGAGTG 
AGTCAGGGAC CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGACGGAG GAGCCTGCAG GTGCCAGCCG CCCCATCCTC CCAGCTGTCC 
TCCTCCTCCT CGGGCGCATC ATCCACATGC ACGGTGTCCA CTGCCAATGT GCTAGTCCTT 
CAAGCTTCTC AGTGCTCCAT GGCCAAGGCC TGCCGGCAGC CACCCATTGT CTTCCTGCCT 
AAGCTCGTGT ATGACATGCT CCTGTCCACT GACAGCAGTG GCTTGCCCAA GTCTGCCTCC 
CTCCTGCCTT CCCCTTCGGT TATGTGGACC AGCTCCTTCC GCCCCCTCCT CAGCAAGATG 
ATGACGTCAA CTGAACAGTC CCTCTACTAC CGGCAGTGGA CAGTGCCCCG GCCCAGCCAC 
ATGGACTACG GCAACAGAGC TGAAGGCCGT GTGGACAGTT TCCACCCACG TAGGCTGCTG 
CTCAGTGGGC CACCTCAGAT TGGGAAGACG GGTGCCTACC TGCAGTTCCT CAGTATCTTG 
TCCAGGATGC TGATTCGGCT GACAGAAGTG GATGTTTACG ATGAAGAAGA GATCAATACC 
GGCCTCCAAG AGGAATCAGA TTGGCATTAC CTCCAGCTCA CCGACCCTTG GCCTGACCTG 
GAGCTCTTCC AGAAGATGCC TTTTGATTAC ATCATTCATG ACCCGAAGTA TGAGGATGCC 
AGTATGATTT GTTCACACCA CCAGAGCATC AAGAGTGAAG ACAGAGGGAT GTCCAGGAAG 
CCAGAGGACC TGTATGTGCG GCGTCAGACG GCACGTATGA GACTGTCTAA GTATGCGGCA 
TACAACACAT ACCACCATTG CGAGCAGTGC CAGCAGTACA TGGGCTTCCA CCCCCACTAC 
CAGCTCTCTG AGTCCACCCT GCACGTCTTT GCCTTCTCTT GCTCCATGCT AGGAGAGGAG 
ATCCAGCTCC ACTTCATCAT CCCCAAGTCC AAGGAGTACC ACTTTGTCTT CAGCCAACCG 
GGAGGCCAGC TGGAGAGCAT GCGCTTGCCC CTCGTCACAG ACAAGAGTTA TGAGCACATC 
AAAAGCCCGA CCTTCACCCC GACGACAGGC CGGCATGAGC ATGGGCTCTT TAATTTGTAC 
CATGCGATGG ATGGAGCCAA CCATCTACAT GTGCTGGTGG TCAAAGAGTA CGAAATGGCC 
ATTTATAAGA AGTACTGGCC CAACCACATC ATGCTTGTCC TCCCAAGCAT CTTTAACAGT 
GCTGGAGTCG GGGCTGCCCA CTTCCTGATC AAAGAGCTGT GCTACCACAA CCTGGAGCTG 
GAGAGGAACC GGCAGGAGGA GCTGGGGGTC AAGCCTCAGG ACATCTGGCC CTTCATCGTC 
ATCTCCGATG ACTCCTGTGT GATGTGGAAT GTTGCAGACG TGGACTGTGC TGGAGACCGG 
AGCAGGGAGT TTTCCTGGTC AGAGAGGAAT GTCTCTCTGA AACACATCAT GCAGCACATT 
GAGGCGTCCC CTAACATCAC ACACTACGCC CTGATCGGCA TGCGGAAGTG GGCCAGCAAG 
ACCCGGAGCC GTGAGGTGCA GGAGCCTTTT TCCCGATGCC ACGTGCATGA CTTCATCATC 
CTCAACGTAG ACTTGACCCA GAATGTACAG TACAACCAGA ATCGGTTTAC ATGTGACGAC 
GTAGACTTCA ACCTTCGCGT GCACAGTGCT GGCCTCCTGC TCTGCCGGTT CAATCGCTTC 
AGCGTCATGA AGAAGCAGAT CGCCGTGGGT GGGCACCGGT CCTTCCACAT CACATCCAAG 
GTGGCTGACA GCTCTGTGGC CATTGTGCCT TCCCAGTACA TCTGTGCCCC GGACAGTAAG 
CACACATTCC TGGCAGCTCC CGCCCAGCTC CTGCTGGAGA AGTTCCTCCA GTACCACAGC 
CACCGCTTCT TCCCGCTGTC TCTGAAGAAC CACAGCCACC CCGTGTTGTC TGTAGACTGC 
TACCTTAACC TGGGGCCTCA GATTTCTGTT TGTTATGTCA GCTCCAGGCC CCACTCCTTA 
AACGTCAGCT GCTCTGACAT GATGTTCAGC GGGCTCCTGC TGTACCTCTG CGACTCTTTT 
GTGGGGGCTA GCTTCTTGAA GAAGTTCCAT TTTCTGAAAG GAGCCACCTT GTGCGTGATC 
TGCCAAGACC GGAACTCGCT TCGACAGACG GTGGTCCGCC TGGAGCTAGA AGAGGAGTGG 
CAGTTCCGGC TGCGGGATGA GTTCCAGACA GCCAATGCCA AGGAAGACAG GCCACTCTTT 
TTCCTCACTG CAAGACACAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762860.1
CDS	1..5844
Translation	MQNNPREEVLHNSIEASLRSNTLVPRPIFSQLYLEAEQQLSSLEGGSRADNEEEEEDGEGGLDPSSPPNAYQLPPPPEGCCTTDGFCQAGKDLRLVSISNEPIEVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQNSTNTCSSSLFPPLESSGSLAAFPTEPAPGTNPSVPVGAQQAGPASDHPSVATATGPAVFNGKDSPKHPQLVKNSLCAAPRPTALGLLPNSGPPKKRHKGWSPESKSTTDGSFIQAVGNRAKHEGASMPCVPQAGLVGPTSVTFPVVASSEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSLSRSYEQHGASTMQPISEETQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQVPPQPFPPATSTAAPVTSAQLPWLAGLAASSCNDSVHVIECAYSLAEGLSEMFRLLIEGKLSKTNYVVIICACRNAAIDSCIAVTGKYQARILSESLLSPAEYQREVHYELVTGKVDSLGAFFSSLCPEGDIDILLDKFHQENQGHVSSSFTASSTKKTAVLDASGVPMCTSYHQEPRGIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTVFHVWHSGVLLELGLEKEPVTKQRAEQHVLKLDTEAQARFKAFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNIYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPISYSNGVDLYHESKKYFGLSEFIDSTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLPQMKNHTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGHPSSGITVGKHFVKQLKMWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNDIPLEKGPKNEALESDGEKLSSTDEDEEAGTEGCFEAGSTSEQRGPVKRERSHSHDSASSSLSSKASGSVLYGESLVQPSGLPQGEFARSSPPCGPAEEGRAPGEIQRLRVSQGPTVISRHSPGLVPQPDSSLRTGRRSLQVPAAPSSQLSSSSSGASSTCTVSTANVLVLQASQCSMAKACRQPPIVFLPKLVYDMLLSTDSSGLPKSASLLPSPSVMWTSSFRPLLSKMMTSTEQSLYYRQWTVPRPSHMDYGNRAEGRVDSFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINTGLQEESDWHYLQLTDPWPDLELFQKMPFDYIIHDPKYEDASMICSHHQSIKSEDRGMSRKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCQQYMGFHPHYQLSESTLHVFAFSCSMLGEEIQLHFIIPKSKEYHFVFSQPGGQLESMRLPLVTDKSYEHIKSPTFTPTTGRHEHGLFNLYHAMDGANHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELCYHNLELERNRQEELGVKPQDIWPFIVISDDSCVMWNVADVDCAGDRSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWASKTRSREVQEPFSRCHVHDFIILNVDLTQNVQYNQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSFHITSKVADSSVAIVPSQYICAPDSKHTFLAAPAQLLLEKFLQYHSHRFFPLSLKNHSHPVLSVDCYLNLGPQISVCYVSSRPHSLNVSCSDMMFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEEEWQFRLRDEFQTANAKEDRPLFFLTARHI

Target ORF information:

RefSeq Version	XM_008764638.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth regulation by estrogen in breast cancer 1 (Greb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764638.1

ORF Insert Sequence:

ATGCAGAACA ATCCCAGAGA AGAGGTCCTC CACAACTCCA TCGAGGCTTC CCTACGCTCC 
AACACCCTGG TGCCCCGGCC CATCTTCTCC CAGCTGTATC TGGAGGCCGA GCAGCAGTTG 
TCATCATTGG AAGGCGGCAG CCGAGCGGAC AATGAGGAAG AAGAAGAAGA TGGGGAAGGA 
GGGCTAGATC CCAGCAGTCC CCCAAATGCC TACCAGCTGC CCCCTCCTCC TGAAGGATGT 
TGCACCACAG ATGGCTTCTG TCAGGCTGGG AAGGACCTAC GTCTTGTCTC CATTTCCAAT 
GAGCCCATTG AAGTGCCTGC AGGCTTCCTC CTGGTGGGTG CCAAGTCTCC CAGCCTGCCA 
GACCACCTCC TGGTGTGTGC AGTGGACAAG AGGTTCTTGC CCGATGACAA TGGCCACAAT 
GCCCTGCTCG GTTTCTCTGG GAACTGTGTT GGCTGTGGGA AAAAAGGCTT CTGCTATTTC 
ACCGAATTCT CCAACCACAT CAACCTGAAG CTGACCACGC AGCCCAAGAA ACAGAAGCAC 
CTCAAGTACT ATCTGGTTCG AAATGCACAA GGGGCACTGA CCAAAGGACC TTTGATTTGC 
TGGAAAGGCT CTGAGTTCAG AAGTCGGCAG AACTCCACCA ACACCTGCTC CAGTTCTCTG 
TTCCCACCAC TGGAGAGTTC AGGGTCCCTG GCCGCCTTCC CCACTGAGCC TGCTCCTGGG 
ACAAACCCCA GTGTCCCAGT GGGAGCTCAA CAGGCAGGAC CAGCCTCTGA TCACCCCTCA 
GTGGCCACAG CAACAGGTCC TGCTGTCTTC AATGGCAAGG ATTCCCCCAA GCATCCACAG 
CTGGTGAAGA ATAGCCTGTG TGCCGCGCCG CGCCCCACAG CCTTAGGTCT CTTGCCAAAC 
TCTGGGCCTC CCAAAAAACG GCACAAAGGG TGGTCCCCAG AGTCTAAATC AACCACAGAT 
GGCAGCTTCA TCCAGGCTGT CGGCAACAGA GCTAAACATG AAGGTGCCAG CATGCCTTGT 
GTACCCCAGG CTGGCTTGGT GGGACCAACT TCAGTCACCT TCCCGGTGGT GGCCTCCAGC 
GAACCAGTGT CAGTTCCGGA TAACTTGCTG AAAATATGCA AGGCCAAGCC AGTGATATTT 
AAAGGCCACG GGAACTTCCC CTACCTCTGC GGAAACCTGA ATGATGTGGT CGTCAGCCCC 
CTACTGTACA CCTGCTACCA GAACTCGCAG TCACTTTCCA GGTCCTATGA GCAACACGGT 
GCCTCCACCA TGCAGCCCAT ATCTGAAGAG ACACAGCTGT TGCTGACCGT CTACTACCTC 
GTCCAGCTGG CTGCAGACCA GGTGCCACTG ATGGAGGACC TGGAGCAGAT CTTCCTGCGC 
TCCTGGCGAG AGTCACATCT GACGGAGATA AGGCAGTACC AGCAGGTGCC TCCACAGCCC 
TTCCCGCCAG CCACCAGCAC AGCAGCGCCT GTGACTTCTG CACAGCTGCC CTGGCTGGCA 
GGCCTGGCCG CCAGCTCCTG CAATGACAGC GTGCATGTTA TCGAATGCGC TTACTCGCTG 
GCTGAGGGCC TCTCCGAGAT GTTCCGGTTG CTCATTGAGG GTAAACTCTC CAAGACCAAC 
TATGTGGTCA TCATCTGTGC CTGCCGAAAT GCCGCCATTG ACTCCTGCAT CGCTGTCACC 
GGGAAATACC AAGCCCGGAT TCTCTCCGAG AGCCTCCTCA GTCCTGCAGA GTACCAGAGA 
GAAGTCCACT ATGAGCTGGT CACTGGCAAA GTGGACTCGC TGGGGGCCTT CTTTAGCAGC 
CTCTGTCCAG AGGGTGACAT TGACATTTTG CTGGACAAAT TTCACCAGGA AAATCAAGGC 
CATGTCTCTT CCTCATTCAC TGCTTCCTCT ACCAAGAAAA CAGCCGTGCT GGATGCCAGT 
GGGGTTCCCA TGTGCACAAG TTACCATCAG GAGCCACGGG GCATCCGGCC CTTTCAGCTG 
GCGGTGGCGC AGAAGCTCCT TTCCCACGTG TGCTCAATCG CAGATTCCAG CACCCAGAAT 
CTGGACTTGG GGTCCTTCGA GAAGGTGGAC TTTCTCATTT GCATTCCCCC ATCAGAGGTG 
ACCTACCAGC AGACTGTGTT CCATGTCTGG CATTCAGGGG TTCTGCTGGA GCTGGGCCTG 
GAGAAAGAAC CTGTTACCAA GCAGAGGGCG GAGCAACACG TCCTGAAACT AGACACGGAG 
GCCCAGGCAA GATTTAAGGC CTTTCTGCAA AACTCCTTCC AGAACCCACA TACGCTCTTT 
GTCCTCATCC ACGACCACGC CCACTGGGAT CTAGTGAGCA GTGCTGTTCA TAACATCTAT 
TCTCAAAGTG ACCCATCTGT GGGATTAGTG GACAGACTGC TCAACTGCCG AGAGGTGAAA 
GAGGCGCCCA ACATTGTGAC TCTCCATGTG ACCTCCTTCC CGTATGCGCT GCAGACTCAG 
CACACGCTCA TCAGCCCCTA CAATGAGATC CACTGGCCCA TCTCCTACAG CAATGGAGTG 
GACCTGTATC ATGAGAGTAA GAAGTACTTT GGGCTGTCTG AGTTTATTGA CTCCACCCTG 
TCAGGACACA GTCTTCCTCT GCTCCGGTAT GACAGCTCCT TTGAGGCCAT GGTCACTGCA 
TTGGGGAAAA GGTTCCCCCG CCTGCACAGC GCGGTGATCA GGACCTTTGT TCTTGTGCAG 
CACTATGCGG CCGCCATGAT GGCCGTGAGT GGCCTCCCAC AGATGAAGAA CCATACATCA 
GTGGAGACCC TAGAGATCAC CCAGAACCTC CTCAACTCCC CAAAGCAGTG CCCCTGTGGC 
CACGGGCTCA TGGTCCTGCT GCGCGTGCCC TGTTCTCCTC TGGCAGCTGT GGCCTATGAG 
CGACTGGCCC ATGTGCGGGC ACGGTTGGCT CTAGAAGAAC ACTTTGAGAT CATTCTGGGC 
CACCCCAGCT CCGGCATCAC TGTAGGCAAG CACTTTGTGA AGCAGCTCAA GATGTGGCAG 
AAAATTGAGG ATGCAGAATG GAGGCCTCAG ACTTACTTGG AGTTGGAGGG CCTGCCCTGC 
ATCCTGATCT TCAGTGGGAT GGACCCTCAT GGGGAGTCGC TGCCACGGTC TCTGAGGTAC 
TGTGACCTGC GTCTAATAAA TTCTTCCTGC CTGGTGAGGA CAGCCCTGGA GCAGGAACTG 
GGTCTGGCTG CCTACTTTGT TAGCAACGAT ATCCCCTTGG AGAAGGGGCC CAAGAATGAG 
GCCTTGGAGA GTGACGGGGA GAAGCTGAGT AGCACAGACG AAGATGAGGA GGCAGGGACA 
GAAGGGTGTT TTGAAGCAGG CTCCACCTCA GAGCAGAGGG GCCCTGTGAA GAGGGAACGT 
TCCCACTCCC ATGACTCCGC ATCTTCATCT CTCTCTTCCA AAGCGTCTGG CTCGGTGCTC 
TATGGTGAGT CCCTGGTTCA GCCCTCAGGA CTTCCACAGG GAGAGTTTGC CCGGTCATCT 
CCACCCTGTG GCCCTGCAGA GGAGGGCAGG GCCCCTGGGG AGATACAGCG GCTCCGAGTG 
AGTCAGGGAC CTACAGTCAT CAGTAGGCAC AGCCCTGGCC TGGTCCCACA GCCTGATTCT 
AGCCTCAGGA CTGGACGGAG GAGCCTGCAG GTGCCAGCCG CCCCATCCTC CCAGCTGTCC 
TCCTCCTCCT CGGGCGCATC ATCCACATGC ACGGTGTCCA CTGCCAATGT GCTAGTCCTT 
CAAGCTTCTC AGTGCTCCAT GGCCAAGGCC TGCCGGCAGC CACCCATTGT CTTCCTGCCT 
AAGCTCGTGT ATGACATGCT CCTGTCCACT GACAGCAGTG GCTTGCCCAA GTCTGCCTCC 
CTCCTGCCTT CCCCTTCGGT TATGTGGACC AGCTCCTTCC GCCCCCTCCT CAGCAAGATG 
ATGACGTCAA CTGAACAGTC CCTCTACTAC CGGCAGTGGA CAGTGCCCCG GCCCAGCCAC 
ATGGACTACG GCAACAGAGC TGAAGGCCGT GTGGACAGTT TCCACCCACG TAGGCTGCTG 
CTCAGTGGGC CACCTCAGAT TGGGAAGACG GGTGCCTACC TGCAGTTCCT CAGTATCTTG 
TCCAGGATGC TGATTCGGCT GACAGAAGTG GATGTTTACG ATGAAGAAGA GATCAATACC 
GGCCTCCAAG AGGAATCAGA TTGGCATTAC CTCCAGCTCA CCGACCCTTG GCCTGACCTG 
GAGCTCTTCC AGAAGATGCC TTTTGATTAC ATCATTCATG ACCCGAAGTA TGAGGATGCC 
AGTATGATTT GTTCACACCA CCAGAGCATC AAGAGTGAAG ACAGAGGGAT GTCCAGGAAG 
CCAGAGGACC TGTATGTGCG GCGTCAGACG GCACGTATGA GACTGTCTAA GTATGCGGCA 
TACAACACAT ACCACCATTG CGAGCAGTGC CAGCAGTACA TGGGCTTCCA CCCCCACTAC 
CAGCTCTCTG AGTCCACCCT GCACGTCTTT GCCTTCTCTT GCTCCATGCT AGGAGAGGAG 
ATCCAGCTCC ACTTCATCAT CCCCAAGTCC AAGGAGTACC ACTTTGTCTT CAGCCAACCG 
GGAGGCCAGC TGGAGAGCAT GCGCTTGCCC CTCGTCACAG ACAAGAGTTA TGAGCACATC 
AAAAGCCCGA CCTTCACCCC GACGACAGGC CGGCATGAGC ATGGGCTCTT TAATTTGTAC 
CATGCGATGG ATGGAGCCAA CCATCTACAT GTGCTGGTGG TCAAAGAGTA CGAAATGGCC 
ATTTATAAGA AGTACTGGCC CAACCACATC ATGCTTGTCC TCCCAAGCAT CTTTAACAGT 
GCTGGAGTCG GGGCTGCCCA CTTCCTGATC AAAGAGCTGT GCTACCACAA CCTGGAGCTG 
GAGAGGAACC GGCAGGAGGA GCTGGGGGTC AAGCCTCAGG ACATCTGGCC CTTCATCGTC 
ATCTCCGATG ACTCCTGTGT GATGTGGAAT GTTGCAGACG TGGACTGTGC TGGAGACCGG 
AGCAGGGAGT TTTCCTGGTC AGAGAGGAAT GTCTCTCTGA AACACATCAT GCAGCACATT 
GAGGCGTCCC CTAACATCAC ACACTACGCC CTGATCGGCA TGCGGAAGTG GGCCAGCAAG 
ACCCGGAGCC GTGAGGTGCA GGAGCCTTTT TCCCGATGCC ACGTGCATGA CTTCATCATC 
CTCAACGTAG ACTTGACCCA GAATGTACAG TACAACCAGA ATCGGTTTAC ATGTGACGAC 
GTAGACTTCA ACCTTCGCGT GCACAGTGCT GGCCTCCTGC TCTGCCGGTT CAATCGCTTC 
AGCGTCATGA AGAAGCAGAT CGCCGTGGGT GGGCACCGGT CCTTCCACAT CACATCCAAG 
GTGGCTGACA GCTCTGTGGC CATTGTGCCT TCCCAGTACA TCTGTGCCCC GGACAGTAAG 
CACACATTCC TGGCAGCTCC CGCCCAGCTC CTGCTGGAGA AGTTCCTCCA GTACCACAGC 
CACCGCTTCT TCCCGCTGTC TCTGAAGAAC CACAGCCACC CCGTGTTGTC TGTAGACTGC 
TACCTTAACC TGGGGCCTCA GATTTCTGTT TGTTATGTCA GCTCCAGGCC CCACTCCTTA 
AACGTCAGCT GCTCTGACAT GATGTTCAGC GGGCTCCTGC TGTACCTCTG CGACTCTTTT 
GTGGGGGCTA GCTTCTTGAA GAAGTTCCAT TTTCTGAAAG GAGCCACCTT GTGCGTGATC 
TGCCAAGACC GGAACTCGCT TCGACAGACG GTGGTCCGCC TGGAGCTAGA AGAGGAGTGG 
CAGTTCCGGC TGCGGGATGA GTTCCAGACA GCCAATGCCA AGGAAGACAG GCCACTCTTT 
TTCCTCACTG CAAGACACAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Greb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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