GREB1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010810181.2	XP_010808483.1	protein GREB1 isoform X1
XM_010810180.2	XP_010808482.1	protein GREB1 isoform X2
NM_001205631.1	NP_001192560.1	protein GREB1
XM_010810188.2	XP_010808490.1	protein GREB1 isoform X3
XM_024998441.1	XP_024854209.1	protein GREB1 isoform X2
XM_024998445.1	XP_024854213.1	protein GREB1 isoform X3
XM_024998446.1	XP_024854214.1	protein GREB1 isoform X3
XM_024998447.1	XP_024854215.1	protein GREB1 isoform X3
XM_024998449.1	XP_024854217.1	protein GREB1 isoform X5
XM_024998444.1	XP_024854212.1	protein GREB1 isoform X3
XM_010810182.2	XP_010808484.1	protein GREB1 isoform X1
XM_024998440.1	XP_024854208.1	protein GREB1 isoform X1
XM_024998443.1	XP_024854211.1	protein GREB1 isoform X3
XM_024998448.1	XP_024854216.1	protein GREB1 isoform X4
XM_005212947.3	XP_005213004.1	protein GREB1 isoform X1
XM_010810181.3	XP_010808483.1	protein GREB1 isoform X3
XM_005212947.4	XP_005213004.1	protein GREB1 isoform X3
XM_010810182.3	XP_010808484.1	protein GREB1 isoform X3
NM_001205631.2	NP_001192560.2	protein GREB1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220214	XM_024998441.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa220215	XM_024998448.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa220216	XM_024998449.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa47082	XM_024998440.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa01629	NM_001205631.1 Latest version!	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	NM_001205631.2 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_024998447.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_010810181.3 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_024998443.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_005212947.4 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_024998444.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_010810182.3 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_024998446.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_024998445.1 Latest version!	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa47083	XM_010810180.2 Latest version!	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa47084	XM_010810188.2 Latest version!	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OBa24163	XM_010810181.2	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_005212947.3	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa24163	XM_010810182.2	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

GREB1 ( XM_024998441.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854209.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220214
Clone ID Related Accession (Same CDS sequence)	XM_024998441.1
Accession Version	XM_024998441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGC GCTGTTCATA ACCTCTACTC CCAAAGTGAC 
CCGTCTGTGG GGCTGGTAGA CAGATTACTC AACTGCAAGG AGGTGAAGGA GGCCCCCAAC 
ATCGTGACAC TCCACGTGAC CTCCTTCCCT TACGCGCTGC AGACGCAGCA CACCCTCGTC 
AGCCCCTACA ACGAGATCCA CTGGCCGGCC TCCTACAGTG ACGGCGTGGA CTTGTATCCT 
GAGAACAAGA AGTACTTCGG GCTGTCTGAG TTCATCGAGT CTACACTCTC AGGACACAGC 
CTCCCATTGC TGAGATACGA CAGCTCCTTT GAGGCCATGG TCACCGCACT GGGAAAAAGG 
TTCCCCCGCC TGCACAGCGC GGTGATCAGG ACCTTTGTTC TCGTGCAGCA CTACGCGGCC 
GCCATGATGG CCGTGAGCGG CCTCTCGCAG ATGAAGAACT ACACGTCCGT GGAGACGCTG 
GAGATCACAC AGAACCTCAT CAACTCCCCG AAGCAGTGCC CCTGCGGCCA CGGGCTCATG 
GTCCTGCTGC GGGTGCCCTG CTCCCCACTG GCGGCCGTGG CCTATGAGCG GCTGGCCCAC 
GTGCGGGCAC GGCTGGCCCT GGAGGAGCAC TTTGAGATCA TCCTGGGGAA CCCCGCCTCG 
GGCATCACCA TCGGGAAGCA CTTCCTGAAG CAGCTCAAGG TGTGGCAGAA CATAGAGGAC 
GCGGAGTGGC GGCCTCAGAC GTACTTGGAG TTGGAGGGCC TGCCTTGCAT CCTGATCTTC 
AGTGGGATGG ACCCACACGG GGAGTCCTTA CCAAGGTCCC TGAGGTACTG TGACCTGCGG 
CTCATAAACT CCTCCTGCCT GGTGAGGACG GCCCTGGAGC AGGAGCTGGG CCTGGCCGCG 
TACTTCGTGA GCAGTGAGGC TCCCCTGGAG AAGGGCGCCA GGACCGAGGC CTTGGAGAGT 
GATGCAGAGA AGCTGAGCAG CACAGACAAC GAGGACGAGG AGCTGGTGAC AGAAGGCTCC 
ACGTCAGAGA AGCGGAGCCC CGTGAAGAGA GAACGATCCT GCTCCCATGA CTCCGCATCT 
TCATCCCTCT CCTCTAAGGC ATCCAGCTCG GCACCATGTG GCGAGTCCTC GGCCGGACCC 
TCGCAGGTGG AGCGGGCCCG GTCCCCACCA TCCTGTGGCC CCTCGGAGGA GGCCAGGGCC 
CCTGGAGAGA GACAGAGGCT CCGGGCAGCG GGGGGGCCAC CGTCGGTCAT CAGCAGGCAC 
AGCCCGGGGC TGACCCCACA GCCGGATCCC AGCCTCAAGA GCGGCCAGCA CGTCCAGGTG 
TCGGCCCCGT CGGCGTCCTC CTCGGGCTCG GCCTCCTCAC CGGCACTGCC CGCCGCCGGC 
GCACTCGTCC TGCAGGCCTC CCAGTGCTCC ATGACCAAGG CCTGCCGGCA GCCGCCCGTG 
GTCTTCCTGC CCAAGCTGGT GTACGACATG GTCACGTCCA CGGATGGCAG CGGCCTGCCC 
AAGGCCGCCT CGCTCCTGCC CTCGCACTCG GTCATGTGGG CCAGCTCCTT CCGGCCCCTG 
CTCAGCAAGA CCATGACGTC CACGGAGCAG TCACTCTACT ACCGCCAGTG GACGGTGCCC 
CGGCCCGGCC ACATGGACTA CGGCAACCGC GCCGAGAGCC GCGTGGACGG CTTCCACCCG 
CGCCGGCTCC TGCTCAGCGG GCCCCCCCAG ATCGGGAAGA CGGGTGCCTA CCTGCAGTTC 
CTCAGCATTT TGTCCAGAAT GCTCATTCGG CTGACGGAAG TGGATGTTTA CGATGAGGAA 
GAGATCAACA TCAACCTCAG AGAAGATTCG GATTGGCATT ACCTCCAGCT CAGTGACCCC 
TGGCCGGACC TGGAGCTGTT CAAGAAGTTG CCTTTTGACT ATATTATTCA TGACCCGAAG 
TATGAGGACG CCAGTCTGAT CTGCTCACAC CATCAGAGTG TAAAGAGTGA AGACAGAGGG 
ACGCACCGGA AGCCGGAGGA CCTGTACGTG CGACGCCAGA CGGCGAGGAT GAGGCTCTCC 
AAGTTCGCAG CGTACAACAC GTACCATCAC TGCGAGCAGT GCCACCAGTA CATGGGCTTC 
CACCCCCGCT ACCAGCTCTA TGAGTCCACC CTGCACGCCT TTGCCTTCTC TTACTCCATG 
CTAGGAGAGG AAATCCAGCT CCACTTCATC ATCCCCAAGT CCAAGGAGCA CCACTTTGTC 
TTCAGCCAAC CCGGAGGCCA GCTGGAGAGC ATGCGACTGC CCCTCGTCAC AGACAAGAGT 
CACGAATACA TAAAGAGCCC GACGTTCACC CCCACAACTG GCCGTCACGA GCATGGACTC 
TTTAACCTGT ACCACGCGAT GGATGGTGCC AGCCACCTGC ATGTGTTGGT TGTCAAGGAG 
TATGAGATGG CGATTTATAA GAAGTACTGG CCCAACCATA TCATGCTGGT CCTTCCCAGC 
ATCTTCAACA GCGCTGGAGT TGGTGCCGCC CACTTCCTGA TCAAGGAGCT GTCTTACCAC 
AACCTGGAGC TGGAGCGGAA CCGTCAGGAG GAGCTGGGAA TCAAGCCCCA GGACATCTGG 
CCTTTCATTG TGATTTCTGA TGACTCCTGC GTGATGTGGA ATGTGGTGGA CGTCGACAGT 
GGTGGGGAGA GAAGCAGAGA ATTTTCCTGG TCGGAGAGAA ACGTGTCCTT GAAGCACATC 
ATGCAGCACA TCGAGGCATC CCCCAACATC ACCCACTACG CCCTGATTGG CATGCGGAAG 
TGGTCCAGCA AGACTGGGAG CCACGAGGTG CGAGAGCCCT TCTCCCGCTG CCATGTGCAT 
GACTTCATCG TCTTGAACGT GGACTTGACC CAGAACGTGC AGTACAACCA GAACCGGTTC 
ATGTGTGACG ACGTGGACTT CAACCTGCGG GTGCACAGCG CCGGCCTCCT GCTCTGCCGG 
TTCAACCGCT TCAGCGTGAT GAAGAAGCAG ATCTCGGTGG GCGGGCACAG GTCCTTCCAC 
ATCACGTCCA AGGTGTCGGA CAGCCCCATG GCCGTCGTGC CCGCCCAGTA CATCTGCGCC 
CCCGACAGCA AGCACACGTT CCTTGCGGCC CCCGCGCAGC TCCTCCTGGA GAAGTTCCTC 
CAGCACCACA GCCACCGCTT CTTCCCGCTG TCCCTGAAAA ATCTCGGCCA CCCTGTGCTG 
TCCGTGGACT GCTACCTGAA CCTGGGGTCT CAGATCTCTG TGTGTTATGT GAGCTCCAGG 
CCCCACTCTT TAAACATCAG CTGCTCTGAC TTCGTGTTCA GTGGACTCCT GCTGTACCTC 
TGTGACTCTT TCGTGGGAGC TAGCTTTTTG AAAAAGTTTC ATTTTCTGAA AGGTGCCACC 
TTGTGTGTGA TCTGTCAGGA CCGCAACTCG CTCCGCCAGA CAGTCGTCCG CCTGGAGCTG 
GAAGACGAGT GGCAGTTTCG GCTGCGAGAT GAATTCCAGA CGGCCAACGC CAAGGAGGAC 
CGGCCACTCT TCTTCCTGAC CGGACGGCAC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854209.1
CDS	95..6010
Translation	MIQLSELWLSSPSSRTVSDWPKATVRVVGMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998441.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998441.1

ORF Insert Sequence:

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGC GCTGTTCATA ACCTCTACTC CCAAAGTGAC 
CCGTCTGTGG GGCTGGTAGA CAGATTACTC AACTGCAAGG AGGTGAAGGA GGCCCCCAAC 
ATCGTGACAC TCCACGTGAC CTCCTTCCCT TACGCGCTGC AGACGCAGCA CACCCTCGTC 
AGCCCCTACA ACGAGATCCA CTGGCCGGCC TCCTACAGTG ACGGCGTGGA CTTGTATCCT 
GAGAACAAGA AGTACTTCGG GCTGTCTGAG TTCATCGAGT CTACACTCTC AGGACACAGC 
CTCCCATTGC TGAGATACGA CAGCTCCTTT GAGGCCATGG TCACCGCACT GGGAAAAAGG 
TTCCCCCGCC TGCACAGCGC GGTGATCAGG ACCTTTGTTC TCGTGCAGCA CTACGCGGCC 
GCCATGATGG CCGTGAGCGG CCTCTCGCAG ATGAAGAACT ACACGTCCGT GGAGACGCTG 
GAGATCACAC AGAACCTCAT CAACTCCCCG AAGCAGTGCC CCTGCGGCCA CGGGCTCATG 
GTCCTGCTGC GGGTGCCCTG CTCCCCACTG GCGGCCGTGG CCTATGAGCG GCTGGCCCAC 
GTGCGGGCAC GGCTGGCCCT GGAGGAGCAC TTTGAGATCA TCCTGGGGAA CCCCGCCTCG 
GGCATCACCA TCGGGAAGCA CTTCCTGAAG CAGCTCAAGG TGTGGCAGAA CATAGAGGAC 
GCGGAGTGGC GGCCTCAGAC GTACTTGGAG TTGGAGGGCC TGCCTTGCAT CCTGATCTTC 
AGTGGGATGG ACCCACACGG GGAGTCCTTA CCAAGGTCCC TGAGGTACTG TGACCTGCGG 
CTCATAAACT CCTCCTGCCT GGTGAGGACG GCCCTGGAGC AGGAGCTGGG CCTGGCCGCG 
TACTTCGTGA GCAGTGAGGC TCCCCTGGAG AAGGGCGCCA GGACCGAGGC CTTGGAGAGT 
GATGCAGAGA AGCTGAGCAG CACAGACAAC GAGGACGAGG AGCTGGTGAC AGAAGGCTCC 
ACGTCAGAGA AGCGGAGCCC CGTGAAGAGA GAACGATCCT GCTCCCATGA CTCCGCATCT 
TCATCCCTCT CCTCTAAGGC ATCCAGCTCG GCACCATGTG GCGAGTCCTC GGCCGGACCC 
TCGCAGGTGG AGCGGGCCCG GTCCCCACCA TCCTGTGGCC CCTCGGAGGA GGCCAGGGCC 
CCTGGAGAGA GACAGAGGCT CCGGGCAGCG GGGGGGCCAC CGTCGGTCAT CAGCAGGCAC 
AGCCCGGGGC TGACCCCACA GCCGGATCCC AGCCTCAAGA GCGGCCAGCA CGTCCAGGTG 
TCGGCCCCGT CGGCGTCCTC CTCGGGCTCG GCCTCCTCAC CGGCACTGCC CGCCGCCGGC 
GCACTCGTCC TGCAGGCCTC CCAGTGCTCC ATGACCAAGG CCTGCCGGCA GCCGCCCGTG 
GTCTTCCTGC CCAAGCTGGT GTACGACATG GTCACGTCCA CGGATGGCAG CGGCCTGCCC 
AAGGCCGCCT CGCTCCTGCC CTCGCACTCG GTCATGTGGG CCAGCTCCTT CCGGCCCCTG 
CTCAGCAAGA CCATGACGTC CACGGAGCAG TCACTCTACT ACCGCCAGTG GACGGTGCCC 
CGGCCCGGCC ACATGGACTA CGGCAACCGC GCCGAGAGCC GCGTGGACGG CTTCCACCCG 
CGCCGGCTCC TGCTCAGCGG GCCCCCCCAG ATCGGGAAGA CGGGTGCCTA CCTGCAGTTC 
CTCAGCATTT TGTCCAGAAT GCTCATTCGG CTGACGGAAG TGGATGTTTA CGATGAGGAA 
GAGATCAACA TCAACCTCAG AGAAGATTCG GATTGGCATT ACCTCCAGCT CAGTGACCCC 
TGGCCGGACC TGGAGCTGTT CAAGAAGTTG CCTTTTGACT ATATTATTCA TGACCCGAAG 
TATGAGGACG CCAGTCTGAT CTGCTCACAC CATCAGAGTG TAAAGAGTGA AGACAGAGGG 
ACGCACCGGA AGCCGGAGGA CCTGTACGTG CGACGCCAGA CGGCGAGGAT GAGGCTCTCC 
AAGTTCGCAG CGTACAACAC GTACCATCAC TGCGAGCAGT GCCACCAGTA CATGGGCTTC 
CACCCCCGCT ACCAGCTCTA TGAGTCCACC CTGCACGCCT TTGCCTTCTC TTACTCCATG 
CTAGGAGAGG AAATCCAGCT CCACTTCATC ATCCCCAAGT CCAAGGAGCA CCACTTTGTC 
TTCAGCCAAC CCGGAGGCCA GCTGGAGAGC ATGCGACTGC CCCTCGTCAC AGACAAGAGT 
CACGAATACA TAAAGAGCCC GACGTTCACC CCCACAACTG GCCGTCACGA GCATGGACTC 
TTTAACCTGT ACCACGCGAT GGATGGTGCC AGCCACCTGC ATGTGTTGGT TGTCAAGGAG 
TATGAGATGG CGATTTATAA GAAGTACTGG CCCAACCATA TCATGCTGGT CCTTCCCAGC 
ATCTTCAACA GCGCTGGAGT TGGTGCCGCC CACTTCCTGA TCAAGGAGCT GTCTTACCAC 
AACCTGGAGC TGGAGCGGAA CCGTCAGGAG GAGCTGGGAA TCAAGCCCCA GGACATCTGG 
CCTTTCATTG TGATTTCTGA TGACTCCTGC GTGATGTGGA ATGTGGTGGA CGTCGACAGT 
GGTGGGGAGA GAAGCAGAGA ATTTTCCTGG TCGGAGAGAA ACGTGTCCTT GAAGCACATC 
ATGCAGCACA TCGAGGCATC CCCCAACATC ACCCACTACG CCCTGATTGG CATGCGGAAG 
TGGTCCAGCA AGACTGGGAG CCACGAGGTG CGAGAGCCCT TCTCCCGCTG CCATGTGCAT 
GACTTCATCG TCTTGAACGT GGACTTGACC CAGAACGTGC AGTACAACCA GAACCGGTTC 
ATGTGTGACG ACGTGGACTT CAACCTGCGG GTGCACAGCG CCGGCCTCCT GCTCTGCCGG 
TTCAACCGCT TCAGCGTGAT GAAGAAGCAG ATCTCGGTGG GCGGGCACAG GTCCTTCCAC 
ATCACGTCCA AGGTGTCGGA CAGCCCCATG GCCGTCGTGC CCGCCCAGTA CATCTGCGCC 
CCCGACAGCA AGCACACGTT CCTTGCGGCC CCCGCGCAGC TCCTCCTGGA GAAGTTCCTC 
CAGCACCACA GCCACCGCTT CTTCCCGCTG TCCCTGAAAA ATCTCGGCCA CCCTGTGCTG 
TCCGTGGACT GCTACCTGAA CCTGGGGTCT CAGATCTCTG TGTGTTATGT GAGCTCCAGG 
CCCCACTCTT TAAACATCAG CTGCTCTGAC TTCGTGTTCA GTGGACTCCT GCTGTACCTC 
TGTGACTCTT TCGTGGGAGC TAGCTTTTTG AAAAAGTTTC ATTTTCTGAA AGGTGCCACC 
TTGTGTGTGA TCTGTCAGGA CCGCAACTCG CTCCGCCAGA CAGTCGTCCG CCTGGAGCTG 
GAAGACGAGT GGCAGTTTCG GCTGCGAGAT GAATTCCAGA CGGCCAACGC CAAGGAGGAC 
CGGCCACTCT TCTTCCTGAC CGGACGGCAC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998448.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854216.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa220215
Clone ID Related Accession (Same CDS sequence)	XM_024998448.1
Accession Version	XM_024998448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGT AGCGCTGTTC ATAACCTCTA CTCCCAAAGT 
GACCCGTCTG TGGGGCTGGT AGACAGATTA CTCAACTGCA AGGAGGTGAA GGAGGCCCCC 
AACATCGTGA CACTCCACGT GACCTCCTTC CCTTACGCGC TGCAGACGCA GCACACCCTC 
GTCAGCCCCT ACAACGAGAT CCACTGGCCG GCCTCCTACA GTGACGGCGT GGACTTGTAT 
CCTGAGAACA AGAAGTACTT CGGGCTGTCT GAGTTCATCG AGTCTACACT CTCAGGACAC 
AGCCTCCCAT TGCTGAGATA CGACAGCTCC TTTGAGGCCA TGGTCACCGC ACTGGGAAAA 
AGGTTCCCCC GCCTGCACAG CGCGGTGATC AGGACCTTTG TTCTCGTGCA GCACTACGCG 
GCCGCCATGA TGGCCGTGAG CGGCCTCTCG CAGATGAAGA ACTACACGTC CGTGGAGACG 
CTGGAGATCA CACAGAACCT CATCAACTCC CCGAAGCAGT GCCCCTGCGG CCACGGGCTC 
ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA CTGGCGGCCG TGGCCTATGA GCGGCTGGCC 
CACGTGCGGG CACGGCTGGC CCTGGAGGAG CACTTTGAGA TCATCCTGGG GAACCCCGCC 
TCGGGCATCA CCATCGGGAA GCACTTCCTG AAGCAGCTCA AGGTGTGGCA GAACATAGAG 
GACGCGGAGT GGCGGCCTCA GACGTACTTG GAGTTGGAGG GCCTGCCTTG CATCCTGATC 
TTCAGTGGGA TGGACCCACA CGGGGAGTCC TTACCAAGGT CCCTGAGGTA CTGTGACCTG 
CGGCTCATAA ACTCCTCCTG CCTGGTGAGG ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC 
GCGTACTTCG TGAGCAGTGA GGCTCCCCTG GAGAAGGGCG CCAGGACCGA GGCCTTGGAG 
AGTGATGCAG AGAAGCTGAG CAGCACAGAC AACGAGGACG AGGAGCTGGT GACAGAAGGC 
TCCACGTCAG AGAAGCGGAG CCCCGTGAAG AGAGAACGAT CCTGCTCCCA TGACTCCGCA 
TCTTCATCCC TCTCCTCTAA GGCATCCAGC TCGGCACCAT GTGGCGAGTC CTCGGCCGGA 
CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA CCATCCTGTG GCCCCTCGGA GGAGGCCAGG 
GCCCCTGGAG AGAGACAGAG GCTCCGGGCA GCGGGGGGGC CACCGTCGGT CATCAGCAGG 
CACAGCCCGG GGCTGACCCC ACAGCCGGAT CCCAGCCTCA AGAGCGGCCA GCACGTCCAG 
GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC TCGGCCTCCT CACCGGCACT GCCCGCCGCC 
GGCGCACTCG TCCTGCAGGC CTCCCAGTGC TCCATGACCA AGGCCTGCCG GCAGCCGCCC 
GTGGTCTTCC TGCCCAAGCT GGTGTACGAC ATGGTCACGT CCACGGATGG CAGCGGCCTG 
CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC TCGGTCATGT GGGCCAGCTC CTTCCGGCCC 
CTGCTCAGCA AGACCATGAC GTCCACGGAG CAGTCACTCT ACTACCGCCA GTGGACGGTG 
CCCCGGCCCG GCCACATGGA CTACGGCAAC CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC 
CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC CAGATCGGGA AGACGGGTGC CTACCTGCAG 
TTCCTCAGCA TTTTGTCCAG AATGCTCATT CGGCTGACGG AAGTGGATGT TTACGATGAG 
GAAGAGATCA ACATCAACCT CAGAGAAGAT TCGGATTGGC ATTACCTCCA GCTCAGTGAC 
CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG TTGCCTTTTG ACTATATTAT TCATGACCCG 
AAGTATGAGG ACGCCAGTCT GATCTGCTCA CACCATCAGA GTGTAAAGAG TGAAGACAGA 
GGGACGCACC GGAAGCCGGA GGACCTGTAC GTGCGACGCC AGACGGCGAG GATGAGGCTC 
TCCAAGTTCG CAGCGTACAA CACGTACCAT CACTGCGAGC AGTGCCACCA GTACATGGGC 
TTCCACCCCC GCTACCAGGA GAGGAAATCC AGCTCCACTT CATCATCCCC AAGTCCAAGG 
AGCACCACTT TGTCTTCAGC CAACCCGGAG GCCAGCTGGA GAGCATGCGA CTGCCCCTCG 
TCACAGACAA GAGTCACGAA TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854216.1
CDS	109..4755
Translation	MIQLSELWLSSPSSRTVSDWPKATVRVVGMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQERKSSSTSSSPSPRSTTLSSANPEASWRACDCPSSQTRVTNT

Target ORF information:

RefSeq Version	XM_024998448.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998448.1

ORF Insert Sequence:

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGT AGCGCTGTTC ATAACCTCTA CTCCCAAAGT 
GACCCGTCTG TGGGGCTGGT AGACAGATTA CTCAACTGCA AGGAGGTGAA GGAGGCCCCC 
AACATCGTGA CACTCCACGT GACCTCCTTC CCTTACGCGC TGCAGACGCA GCACACCCTC 
GTCAGCCCCT ACAACGAGAT CCACTGGCCG GCCTCCTACA GTGACGGCGT GGACTTGTAT 
CCTGAGAACA AGAAGTACTT CGGGCTGTCT GAGTTCATCG AGTCTACACT CTCAGGACAC 
AGCCTCCCAT TGCTGAGATA CGACAGCTCC TTTGAGGCCA TGGTCACCGC ACTGGGAAAA 
AGGTTCCCCC GCCTGCACAG CGCGGTGATC AGGACCTTTG TTCTCGTGCA GCACTACGCG 
GCCGCCATGA TGGCCGTGAG CGGCCTCTCG CAGATGAAGA ACTACACGTC CGTGGAGACG 
CTGGAGATCA CACAGAACCT CATCAACTCC CCGAAGCAGT GCCCCTGCGG CCACGGGCTC 
ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA CTGGCGGCCG TGGCCTATGA GCGGCTGGCC 
CACGTGCGGG CACGGCTGGC CCTGGAGGAG CACTTTGAGA TCATCCTGGG GAACCCCGCC 
TCGGGCATCA CCATCGGGAA GCACTTCCTG AAGCAGCTCA AGGTGTGGCA GAACATAGAG 
GACGCGGAGT GGCGGCCTCA GACGTACTTG GAGTTGGAGG GCCTGCCTTG CATCCTGATC 
TTCAGTGGGA TGGACCCACA CGGGGAGTCC TTACCAAGGT CCCTGAGGTA CTGTGACCTG 
CGGCTCATAA ACTCCTCCTG CCTGGTGAGG ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC 
GCGTACTTCG TGAGCAGTGA GGCTCCCCTG GAGAAGGGCG CCAGGACCGA GGCCTTGGAG 
AGTGATGCAG AGAAGCTGAG CAGCACAGAC AACGAGGACG AGGAGCTGGT GACAGAAGGC 
TCCACGTCAG AGAAGCGGAG CCCCGTGAAG AGAGAACGAT CCTGCTCCCA TGACTCCGCA 
TCTTCATCCC TCTCCTCTAA GGCATCCAGC TCGGCACCAT GTGGCGAGTC CTCGGCCGGA 
CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA CCATCCTGTG GCCCCTCGGA GGAGGCCAGG 
GCCCCTGGAG AGAGACAGAG GCTCCGGGCA GCGGGGGGGC CACCGTCGGT CATCAGCAGG 
CACAGCCCGG GGCTGACCCC ACAGCCGGAT CCCAGCCTCA AGAGCGGCCA GCACGTCCAG 
GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC TCGGCCTCCT CACCGGCACT GCCCGCCGCC 
GGCGCACTCG TCCTGCAGGC CTCCCAGTGC TCCATGACCA AGGCCTGCCG GCAGCCGCCC 
GTGGTCTTCC TGCCCAAGCT GGTGTACGAC ATGGTCACGT CCACGGATGG CAGCGGCCTG 
CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC TCGGTCATGT GGGCCAGCTC CTTCCGGCCC 
CTGCTCAGCA AGACCATGAC GTCCACGGAG CAGTCACTCT ACTACCGCCA GTGGACGGTG 
CCCCGGCCCG GCCACATGGA CTACGGCAAC CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC 
CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC CAGATCGGGA AGACGGGTGC CTACCTGCAG 
TTCCTCAGCA TTTTGTCCAG AATGCTCATT CGGCTGACGG AAGTGGATGT TTACGATGAG 
GAAGAGATCA ACATCAACCT CAGAGAAGAT TCGGATTGGC ATTACCTCCA GCTCAGTGAC 
CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG TTGCCTTTTG ACTATATTAT TCATGACCCG 
AAGTATGAGG ACGCCAGTCT GATCTGCTCA CACCATCAGA GTGTAAAGAG TGAAGACAGA 
GGGACGCACC GGAAGCCGGA GGACCTGTAC GTGCGACGCC AGACGGCGAG GATGAGGCTC 
TCCAAGTTCG CAGCGTACAA CACGTACCAT CACTGCGAGC AGTGCCACCA GTACATGGGC 
TTCCACCCCC GCTACCAGGA GAGGAAATCC AGCTCCACTT CATCATCCCC AAGTCCAAGG 
AGCACCACTT TGTCTTCAGC CAACCCGGAG GCCAGCTGGA GAGCATGCGA CTGCCCCTCG 
TCACAGACAA GAGTCACGAA TACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998449.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854217.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa220216
Clone ID Related Accession (Same CDS sequence)	XM_024998449.1
Accession Version	XM_024998449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGT AGCGCTGTTC ATAACCTCTA CTCCCAAAGT 
GACCCGTCTG TGGGGCTGGT AGACAGATTA CTCAACTGCA AGGAGGTGAA GGAGGCCCCC 
AACATCGTGA CACTCCACGT GACCTCCTTC CCTTACGCGC TGCAGACGCA GCACACCCTC 
GTCAGCCCCT ACAACGAGAT CCACTGGCCG GCCTCCTACA GTGACGGCGT GGACTTGTAT 
CCTGAGAACA AGAAGTACTT CGGGCTGTCT GAGTTCATCG AGTCTACACT CTCAGGACAC 
AGCCTCCCAT TGCTGAGATA CGACAGCTCC TTTGAGGCCA TGGTCACCGC ACTGGGAAAA 
AGGTTCCCCC GCCTGCACAG CGCGGTGATC AGGACCTTTG TTCTCGTGCA GCACTACGCG 
GCCGCCATGA TGGCCGTGAG CGGCCTCTCG CAGATGAAGA ACTACACGTC CGTGGAGACG 
CTGGAGATCA CACAGAACCT CATCAACTCC CCGAAGCAGT GCCCCTGCGG CCACGGGCTC 
ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA CTGGCGGCCG TGGCCTATGA GCGGCTGGCC 
CACGTGCGGG CACGGCTGGC CCTGGAGGAG CACTTTGAGA TCATCCTGGG GAACCCCGCC 
TCGGGCATCA CCATCGGGAA GCACTTCCTG AAGCAGCTCA AGGTGTGGCA GAACATAGAG 
GACGCGGAGT GGCGGCCTCA GACGTACTTG GAGTTGGAGG GCCTGCCTTG CATCCTGATC 
TTCAGTGGGA TGGACCCACA CGGGGAGTCC TTACCAAGGT CCCTGAGGTA CTGTGACCTG 
CGGCTCATAA ACTCCTCCTG CCTGGTGAGG ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC 
GCGTACTTCG TGAGCAGTGA GGCTCCCCTG GAGAAGGGCG CCAGGACCGA GGCCTTGGAG 
AGTGATGCAG AGAAGCTGAG CAGCACAGAC AACGAGGACG AGGAGCTGGT GACAGAAGGC 
TCCACGTCAG AGAAGCGGAG CCCCGTGAAG AGAGAACGAT CCTGCTCCCA TGACTCCGCA 
TCTTCATCCC TCTCCTCTAA GGCATCCAGC TCGGCACCAT GTGGCGAGTC CTCGGCCGGA 
CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA CCATCCTGTG GCCCCTCGGA GGAGGCCAGG 
GCCCCTGGAG AGAGACAGAG GCTCCGGGCA GCGGGGGGGC CACCGTCGGT CATCAGCAGG 
CACAGCCCGG GGCTGACCCC ACAGCCGGAT CCCAGCCTCA AGAGCGGCCA GCACGTCCAG 
GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC TCGGCCTCCT CACCGGCACT GCCCGCCGCC 
GGCGCACTCG TCCTGCAGGC CTCCCAGTGC TCCATGACCA AGGCCTGCCG GCAGCCGCCC 
GTGGTCTTCC TGCCCAAGCT GGTGTACGAC ATGGTCACGT CCACGGATGG CAGCGGCCTG 
CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC TCGGTCATGT GGGCCAGCTC CTTCCGGCCC 
CTGCTCAGCA AGACCATGAC GTCCACGGAG CAGTCACTCT ACTACCGCCA GTGGACGGTG 
CCCCGGCCCG GCCACATGGA CTACGGCAAC CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC 
CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC CAGATCGGGA AGACGGGTGC CTACCTGCAG 
TTCCTCAGCA TTTTGTCCAG AATGCTCATT CGGCTGACGG AAGTGGATGT TTACGATGAG 
GAAGAGATCA ACATCAACCT CAGAGAAGAT TCGGATTGGC ATTACCTCCA GCTCAGTGAC 
CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG TTGCCTTTTG ACTATATTAT TCATGACCCG 
AAGTATGAGG ACGCCAGTCT GATCTGCTCA CACCATCAGA GTGTAAAGAG TGAAGACAGA 
GGGACGCACC GGAAGCCGGA GGACCTGTAC GTGCGACGCC AGACGGCGAG GATGAGGCTC 
TCCAAGTTCG CAGCGTACAA CACGTACCAT CACTGCGAGC AGTGCCACCA GTACATGGGC 
TTCCACCCCC GCTACCAGAT GCAGGCAAGG AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854217.1
CDS	109..4644
Translation	MIQLSELWLSSPSSRTVSDWPKATVRVVGMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQMQARK

Target ORF information:

RefSeq Version	XM_024998449.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998449.1

ORF Insert Sequence:

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGT AGCGCTGTTC ATAACCTCTA CTCCCAAAGT 
GACCCGTCTG TGGGGCTGGT AGACAGATTA CTCAACTGCA AGGAGGTGAA GGAGGCCCCC 
AACATCGTGA CACTCCACGT GACCTCCTTC CCTTACGCGC TGCAGACGCA GCACACCCTC 
GTCAGCCCCT ACAACGAGAT CCACTGGCCG GCCTCCTACA GTGACGGCGT GGACTTGTAT 
CCTGAGAACA AGAAGTACTT CGGGCTGTCT GAGTTCATCG AGTCTACACT CTCAGGACAC 
AGCCTCCCAT TGCTGAGATA CGACAGCTCC TTTGAGGCCA TGGTCACCGC ACTGGGAAAA 
AGGTTCCCCC GCCTGCACAG CGCGGTGATC AGGACCTTTG TTCTCGTGCA GCACTACGCG 
GCCGCCATGA TGGCCGTGAG CGGCCTCTCG CAGATGAAGA ACTACACGTC CGTGGAGACG 
CTGGAGATCA CACAGAACCT CATCAACTCC CCGAAGCAGT GCCCCTGCGG CCACGGGCTC 
ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA CTGGCGGCCG TGGCCTATGA GCGGCTGGCC 
CACGTGCGGG CACGGCTGGC CCTGGAGGAG CACTTTGAGA TCATCCTGGG GAACCCCGCC 
TCGGGCATCA CCATCGGGAA GCACTTCCTG AAGCAGCTCA AGGTGTGGCA GAACATAGAG 
GACGCGGAGT GGCGGCCTCA GACGTACTTG GAGTTGGAGG GCCTGCCTTG CATCCTGATC 
TTCAGTGGGA TGGACCCACA CGGGGAGTCC TTACCAAGGT CCCTGAGGTA CTGTGACCTG 
CGGCTCATAA ACTCCTCCTG CCTGGTGAGG ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC 
GCGTACTTCG TGAGCAGTGA GGCTCCCCTG GAGAAGGGCG CCAGGACCGA GGCCTTGGAG 
AGTGATGCAG AGAAGCTGAG CAGCACAGAC AACGAGGACG AGGAGCTGGT GACAGAAGGC 
TCCACGTCAG AGAAGCGGAG CCCCGTGAAG AGAGAACGAT CCTGCTCCCA TGACTCCGCA 
TCTTCATCCC TCTCCTCTAA GGCATCCAGC TCGGCACCAT GTGGCGAGTC CTCGGCCGGA 
CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA CCATCCTGTG GCCCCTCGGA GGAGGCCAGG 
GCCCCTGGAG AGAGACAGAG GCTCCGGGCA GCGGGGGGGC CACCGTCGGT CATCAGCAGG 
CACAGCCCGG GGCTGACCCC ACAGCCGGAT CCCAGCCTCA AGAGCGGCCA GCACGTCCAG 
GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC TCGGCCTCCT CACCGGCACT GCCCGCCGCC 
GGCGCACTCG TCCTGCAGGC CTCCCAGTGC TCCATGACCA AGGCCTGCCG GCAGCCGCCC 
GTGGTCTTCC TGCCCAAGCT GGTGTACGAC ATGGTCACGT CCACGGATGG CAGCGGCCTG 
CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC TCGGTCATGT GGGCCAGCTC CTTCCGGCCC 
CTGCTCAGCA AGACCATGAC GTCCACGGAG CAGTCACTCT ACTACCGCCA GTGGACGGTG 
CCCCGGCCCG GCCACATGGA CTACGGCAAC CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC 
CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC CAGATCGGGA AGACGGGTGC CTACCTGCAG 
TTCCTCAGCA TTTTGTCCAG AATGCTCATT CGGCTGACGG AAGTGGATGT TTACGATGAG 
GAAGAGATCA ACATCAACCT CAGAGAAGAT TCGGATTGGC ATTACCTCCA GCTCAGTGAC 
CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG TTGCCTTTTG ACTATATTAT TCATGACCCG 
AAGTATGAGG ACGCCAGTCT GATCTGCTCA CACCATCAGA GTGTAAAGAG TGAAGACAGA 
GGGACGCACC GGAAGCCGGA GGACCTGTAC GTGCGACGCC AGACGGCGAG GATGAGGCTC 
TCCAAGTTCG CAGCGTACAA CACGTACCAT CACTGCGAGC AGTGCCACCA GTACATGGGC 
TTCCACCCCC GCTACCAGAT GCAGGCAAGG AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998440.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854208.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X1, mRNA.

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CloneID	OBa47082
Clone ID Related Accession (Same CDS sequence)	XM_024998440.1
Accession Version	XM_024998440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGT AGCGCTGTTC ATAACCTCTA CTCCCAAAGT 
GACCCGTCTG TGGGGCTGGT AGACAGATTA CTCAACTGCA AGGAGGTGAA GGAGGCCCCC 
AACATCGTGA CACTCCACGT GACCTCCTTC CCTTACGCGC TGCAGACGCA GCACACCCTC 
GTCAGCCCCT ACAACGAGAT CCACTGGCCG GCCTCCTACA GTGACGGCGT GGACTTGTAT 
CCTGAGAACA AGAAGTACTT CGGGCTGTCT GAGTTCATCG AGTCTACACT CTCAGGACAC 
AGCCTCCCAT TGCTGAGATA CGACAGCTCC TTTGAGGCCA TGGTCACCGC ACTGGGAAAA 
AGGTTCCCCC GCCTGCACAG CGCGGTGATC AGGACCTTTG TTCTCGTGCA GCACTACGCG 
GCCGCCATGA TGGCCGTGAG CGGCCTCTCG CAGATGAAGA ACTACACGTC CGTGGAGACG 
CTGGAGATCA CACAGAACCT CATCAACTCC CCGAAGCAGT GCCCCTGCGG CCACGGGCTC 
ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA CTGGCGGCCG TGGCCTATGA GCGGCTGGCC 
CACGTGCGGG CACGGCTGGC CCTGGAGGAG CACTTTGAGA TCATCCTGGG GAACCCCGCC 
TCGGGCATCA CCATCGGGAA GCACTTCCTG AAGCAGCTCA AGGTGTGGCA GAACATAGAG 
GACGCGGAGT GGCGGCCTCA GACGTACTTG GAGTTGGAGG GCCTGCCTTG CATCCTGATC 
TTCAGTGGGA TGGACCCACA CGGGGAGTCC TTACCAAGGT CCCTGAGGTA CTGTGACCTG 
CGGCTCATAA ACTCCTCCTG CCTGGTGAGG ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC 
GCGTACTTCG TGAGCAGTGA GGCTCCCCTG GAGAAGGGCG CCAGGACCGA GGCCTTGGAG 
AGTGATGCAG AGAAGCTGAG CAGCACAGAC AACGAGGACG AGGAGCTGGT GACAGAAGGC 
TCCACGTCAG AGAAGCGGAG CCCCGTGAAG AGAGAACGAT CCTGCTCCCA TGACTCCGCA 
TCTTCATCCC TCTCCTCTAA GGCATCCAGC TCGGCACCAT GTGGCGAGTC CTCGGCCGGA 
CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA CCATCCTGTG GCCCCTCGGA GGAGGCCAGG 
GCCCCTGGAG AGAGACAGAG GCTCCGGGCA GCGGGGGGGC CACCGTCGGT CATCAGCAGG 
CACAGCCCGG GGCTGACCCC ACAGCCGGAT CCCAGCCTCA AGAGCGGCCA GCACGTCCAG 
GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC TCGGCCTCCT CACCGGCACT GCCCGCCGCC 
GGCGCACTCG TCCTGCAGGC CTCCCAGTGC TCCATGACCA AGGCCTGCCG GCAGCCGCCC 
GTGGTCTTCC TGCCCAAGCT GGTGTACGAC ATGGTCACGT CCACGGATGG CAGCGGCCTG 
CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC TCGGTCATGT GGGCCAGCTC CTTCCGGCCC 
CTGCTCAGCA AGACCATGAC GTCCACGGAG CAGTCACTCT ACTACCGCCA GTGGACGGTG 
CCCCGGCCCG GCCACATGGA CTACGGCAAC CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC 
CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC CAGATCGGGA AGACGGGTGC CTACCTGCAG 
TTCCTCAGCA TTTTGTCCAG AATGCTCATT CGGCTGACGG AAGTGGATGT TTACGATGAG 
GAAGAGATCA ACATCAACCT CAGAGAAGAT TCGGATTGGC ATTACCTCCA GCTCAGTGAC 
CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG TTGCCTTTTG ACTATATTAT TCATGACCCG 
AAGTATGAGG ACGCCAGTCT GATCTGCTCA CACCATCAGA GTGTAAAGAG TGAAGACAGA 
GGGACGCACC GGAAGCCGGA GGACCTGTAC GTGCGACGCC AGACGGCGAG GATGAGGCTC 
TCCAAGTTCG CAGCGTACAA CACGTACCAT CACTGCGAGC AGTGCCACCA GTACATGGGC 
TTCCACCCCC GCTACCAGCT CTATGAGTCC ACCCTGCACG CCTTTGCCTT CTCTTACTCC 
ATGCTAGGAG AGGAAATCCA GCTCCACTTC ATCATCCCCA AGTCCAAGGA GCACCACTTT 
GTCTTCAGCC AACCCGGAGG CCAGCTGGAG AGCATGCGAC TGCCCCTCGT CACAGACAAG 
AGTCACGAAT ACATAAAGAG CCCGACGTTC ACCCCCACAA CTGGCCGTCA CGAGCATGGA 
CTCTTTAACC TGTACCACGC GATGGATGGT GCCAGCCACC TGCATGTGTT GGTTGTCAAG 
GAGTATGAGA TGGCGATTTA TAAGAAGTAC TGGCCCAACC ATATCATGCT GGTCCTTCCC 
AGCATCTTCA ACAGCGCTGG AGTTGGTGCC GCCCACTTCC TGATCAAGGA GCTGTCTTAC 
CACAACCTGG AGCTGGAGCG GAACCGTCAG GAGGAGCTGG GAATCAAGCC CCAGGACATC 
TGGCCTTTCA TTGTGATTTC TGATGACTCC TGCGTGATGT GGAATGTGGT GGACGTCGAC 
AGTGGTGGGG AGAGAAGCAG AGAATTTTCC TGGTCGGAGA GAAACGTGTC CTTGAAGCAC 
ATCATGCAGC ACATCGAGGC ATCCCCCAAC ATCACCCACT ACGCCCTGAT TGGCATGCGG 
AAGTGGTCCA GCAAGACTGG GAGCCACGAG GTGCGAGAGC CCTTCTCCCG CTGCCATGTG 
CATGACTTCA TCGTCTTGAA CGTGGACTTG ACCCAGAACG TGCAGTACAA CCAGAACCGG 
TTCATGTGTG ACGACGTGGA CTTCAACCTG CGGGTGCACA GCGCCGGCCT CCTGCTCTGC 
CGGTTCAACC GCTTCAGCGT GATGAAGAAG CAGATCTCGG TGGGCGGGCA CAGGTCCTTC 
CACATCACGT CCAAGGTGTC GGACAGCCCC ATGGCCGTCG TGCCCGCCCA GTACATCTGC 
GCCCCCGACA GCAAGCACAC GTTCCTTGCG GCCCCCGCGC AGCTCCTCCT GGAGAAGTTC 
CTCCAGCACC ACAGCCACCG CTTCTTCCCG CTGTCCCTGA AAAATCTCGG CCACCCTGTG 
CTGTCCGTGG ACTGCTACCT GAACCTGGGG TCTCAGATCT CTGTGTGTTA TGTGAGCTCC 
AGGCCCCACT CTTTAAACAT CAGCTGCTCT GACTTCGTGT TCAGTGGACT CCTGCTGTAC 
CTCTGTGACT CTTTCGTGGG AGCTAGCTTT TTGAAAAAGT TTCATTTTCT GAAAGGTGCC 
ACCTTGTGTG TGATCTGTCA GGACCGCAAC TCGCTCCGCC AGACAGTCGT CCGCCTGGAG 
CTGGAAGACG AGTGGCAGTT TCGGCTGCGA GATGAATTCC AGACGGCCAA CGCCAAGGAG 
GACCGGCCAC TCTTCTTCCT GACCGGACGG CACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854208.1
CDS	93..6011
Translation	MIQLSELWLSSPSSRTVSDWPKATVRVVGMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998440.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998440.1

ORF Insert Sequence:

ATGATTCAGC TCAGTGAACT GTGGCTGTCT TCCCCATCCT CAAGGACAGT GTCAGACTGG 
CCCAAGGCCA CGGTGCGAGT CGTGGGGATG GGAAACTCGT ACGCTGGACA GCTGAAGACC 
ACGCGGTTCG AGGAGGTCCT GCACAACTCC ATCGAGGCCT CCCTGCGCTC CAACAGCCTG 
GTACCCAGGC CCATCTTCTC CCAGTTGTAC CTAGAAGCAG AGCAGCAGCT TTCCGCTCTA 
GAAGGTGGAA GCCGAGTGGA CAATGAAGAA GAGGAAGAGG AAGGGGACGG GGGGCTGGAA 
CAGAACTGTC CCCCAAACCC CTACCAGATG CACCCCCCGC CTGAAGGATG CTGCACCACG 
GACGGGTTCT GCCAGGCCGG GAAGGACCTG CGTCTGGTCT CCATCTCCAG TGAGCCCATC 
GACATCCCTG CGGGCTTCCT CCTGGTGGGT GCCAAGTCCC CCAGCCTGCC TGACCATCTC 
CTGGTGTGTG CCGTGGACAA GAGGTTCCTG CCGGATGACA ACGGACACAA CGCCCTTCTT 
GGTTTCTCTG GGAATTGTGT CGGCTGTGGC AAGAAGGGCT TCTGTTACTT CACGGAGTTC 
TCCAATCACA TCAATCTGAA GCTGACCACG CAGCCCAAGA AGCAGAAACA CTTGAAATAC 
TACCTGGTCC GCAACGCCCA GGGGGCTCTG ACCAAAGGGC CTCTCATTTG CTGGAAAGGC 
TCAGAGTTCA GAAGCCGGCA GGCATCCATG GGTGCATGCT CCAGTTCTCT GTTCCCGCCA 
CTGGACAGCT CTGGGCCGCC GGCCCCCTTC CCTGGTGAGC CCGGTCCGGG GACAGCCCCG 
AGCGCCCCGC TGGGAGCCCC GCAGGCAGGA CCAGCCTCCG AGCACCCCGC CCTAACCGCA 
GCCCTGGGTC TGGCTGTTTT CAACGGCAAA GATTCCCCGA AGCACCAGCC GCTGGTGAAG 
AACCGCCTGT CTGCAGTGCC GCGTCCCTCG GCCTTAGGTA TCTTATCAAA CTCTGGCCCT 
CCCAAAAAAC GCCACAAAGG CTGGTCTCCA GAATCTCCAT CAGCTTCGGA TGGTGGCTAC 
CCCCAGGGAG GGGGGCACAG AGCCAAGTAT GAAAGTGCAG GTGTGACCTG CATGCCCCAG 
GTCGGCTTGG TGGGACCGGC TTCAGTCACC TTTCCCGTTG TGGCTTCTGG AGAACCAGTG 
TCCGTTCCCG ACAACTTGTT GAAGATATGC AAGGCCAAGC CAGTGATCTT TAAAGGCCAC 
GGGAACTTCC CCTACCTCTG CGGCAACCTG AACGACGTGG TTGTGAGCCC CCTGCTGTAC 
ACGTGCTACC AGAACTCGCA GTCCATCTCC AGAGCCTACG AGCAGTACGG GGCCTCCACC 
ATTCAGCCCA TCTCGGAGGA GATGCAGCTC CTGCTGACCG TCTACTATCT CGTCCAGCTG 
GCGGCGGACC AGGTGCCCCT GATGGAGGAC CTGGAGCAGA TCTTCCTGCG GTCGTGGCGT 
GAGTCGCATC TAACGGAGAT CCGGCAGTAC CAGCAGGCGC CGCCCCAGCC CTTCCCACCT 
GTGCCGGGTG CTGTGGCGCC GGTGACCTCC GCGCAGCTGC CCTGGCTGGC CGGCCTGGCT 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCTCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTGATCGAG GGCAAGCTGG CCAAGACCAA CTACGTGGTG 
ATCATCTGCG CCTGCCGCAG CGCCGCCATC GACTCCTGCA TTGCTGTCAC CGGAAAATAC 
CAAGCCCGGA TTCTTTCTGA GAGCCTTCTC ACCCCAGCTG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACTGGGAA AGTGGACTCG CTAGGGGCCT TTTTTAGCAC CCTCTGTCCA 
GAAGGCGACA TTGACATTTT GCTGGACAAA TTCCATCAGG AAAATCAAGG CCTTGTTTCT 
TCCTCACTTA CTGCCTCCTC TGTCACCAAG TCAGCATCCC TGGATGTCAG CGGGGCACCT 
GCGTGCACAA GTTACCATCT GGAGCCGCAC CGCATCCGGC CCTTCCAGCT GGCTGTGGCC 
CAGAAGCTCC TCTCCCATGT GTGTTCAATC GCGGATTCCA GCACCCAGAA TCTGGACTTG 
GGATCCTTTG AGAAGGTGGA CTTTCTGATT TGTATTCCAC CCTCAGAAGT GACTTACCAG 
CAGACCCTCT TCCATGTCTG GCATTCAGGG GTTTTGCTGG AGCTGGGCTT GGAGAAGGAG 
CACGTGACCA GGCAGAGGGT GGAGCAATAT GTTCTGAAGC TTGATGCCGA AGCACAGACG 
AAGTTCAAGT CCTTCCTGCA AAACTCCTTT CAGAATCCGC ACACACTCTT CGTCCTCATC 
CACGACCATG CCCACTGGGA TCTTGTGAGT AGCGCTGTTC ATAACCTCTA CTCCCAAAGT 
GACCCGTCTG TGGGGCTGGT AGACAGATTA CTCAACTGCA AGGAGGTGAA GGAGGCCCCC 
AACATCGTGA CACTCCACGT GACCTCCTTC CCTTACGCGC TGCAGACGCA GCACACCCTC 
GTCAGCCCCT ACAACGAGAT CCACTGGCCG GCCTCCTACA GTGACGGCGT GGACTTGTAT 
CCTGAGAACA AGAAGTACTT CGGGCTGTCT GAGTTCATCG AGTCTACACT CTCAGGACAC 
AGCCTCCCAT TGCTGAGATA CGACAGCTCC TTTGAGGCCA TGGTCACCGC ACTGGGAAAA 
AGGTTCCCCC GCCTGCACAG CGCGGTGATC AGGACCTTTG TTCTCGTGCA GCACTACGCG 
GCCGCCATGA TGGCCGTGAG CGGCCTCTCG CAGATGAAGA ACTACACGTC CGTGGAGACG 
CTGGAGATCA CACAGAACCT CATCAACTCC CCGAAGCAGT GCCCCTGCGG CCACGGGCTC 
ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA CTGGCGGCCG TGGCCTATGA GCGGCTGGCC 
CACGTGCGGG CACGGCTGGC CCTGGAGGAG CACTTTGAGA TCATCCTGGG GAACCCCGCC 
TCGGGCATCA CCATCGGGAA GCACTTCCTG AAGCAGCTCA AGGTGTGGCA GAACATAGAG 
GACGCGGAGT GGCGGCCTCA GACGTACTTG GAGTTGGAGG GCCTGCCTTG CATCCTGATC 
TTCAGTGGGA TGGACCCACA CGGGGAGTCC TTACCAAGGT CCCTGAGGTA CTGTGACCTG 
CGGCTCATAA ACTCCTCCTG CCTGGTGAGG ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC 
GCGTACTTCG TGAGCAGTGA GGCTCCCCTG GAGAAGGGCG CCAGGACCGA GGCCTTGGAG 
AGTGATGCAG AGAAGCTGAG CAGCACAGAC AACGAGGACG AGGAGCTGGT GACAGAAGGC 
TCCACGTCAG AGAAGCGGAG CCCCGTGAAG AGAGAACGAT CCTGCTCCCA TGACTCCGCA 
TCTTCATCCC TCTCCTCTAA GGCATCCAGC TCGGCACCAT GTGGCGAGTC CTCGGCCGGA 
CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA CCATCCTGTG GCCCCTCGGA GGAGGCCAGG 
GCCCCTGGAG AGAGACAGAG GCTCCGGGCA GCGGGGGGGC CACCGTCGGT CATCAGCAGG 
CACAGCCCGG GGCTGACCCC ACAGCCGGAT CCCAGCCTCA AGAGCGGCCA GCACGTCCAG 
GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC TCGGCCTCCT CACCGGCACT GCCCGCCGCC 
GGCGCACTCG TCCTGCAGGC CTCCCAGTGC TCCATGACCA AGGCCTGCCG GCAGCCGCCC 
GTGGTCTTCC TGCCCAAGCT GGTGTACGAC ATGGTCACGT CCACGGATGG CAGCGGCCTG 
CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC TCGGTCATGT GGGCCAGCTC CTTCCGGCCC 
CTGCTCAGCA AGACCATGAC GTCCACGGAG CAGTCACTCT ACTACCGCCA GTGGACGGTG 
CCCCGGCCCG GCCACATGGA CTACGGCAAC CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC 
CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC CAGATCGGGA AGACGGGTGC CTACCTGCAG 
TTCCTCAGCA TTTTGTCCAG AATGCTCATT CGGCTGACGG AAGTGGATGT TTACGATGAG 
GAAGAGATCA ACATCAACCT CAGAGAAGAT TCGGATTGGC ATTACCTCCA GCTCAGTGAC 
CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG TTGCCTTTTG ACTATATTAT TCATGACCCG 
AAGTATGAGG ACGCCAGTCT GATCTGCTCA CACCATCAGA GTGTAAAGAG TGAAGACAGA 
GGGACGCACC GGAAGCCGGA GGACCTGTAC GTGCGACGCC AGACGGCGAG GATGAGGCTC 
TCCAAGTTCG CAGCGTACAA CACGTACCAT CACTGCGAGC AGTGCCACCA GTACATGGGC 
TTCCACCCCC GCTACCAGCT CTATGAGTCC ACCCTGCACG CCTTTGCCTT CTCTTACTCC 
ATGCTAGGAG AGGAAATCCA GCTCCACTTC ATCATCCCCA AGTCCAAGGA GCACCACTTT 
GTCTTCAGCC AACCCGGAGG CCAGCTGGAG AGCATGCGAC TGCCCCTCGT CACAGACAAG 
AGTCACGAAT ACATAAAGAG CCCGACGTTC ACCCCCACAA CTGGCCGTCA CGAGCATGGA 
CTCTTTAACC TGTACCACGC GATGGATGGT GCCAGCCACC TGCATGTGTT GGTTGTCAAG 
GAGTATGAGA TGGCGATTTA TAAGAAGTAC TGGCCCAACC ATATCATGCT GGTCCTTCCC 
AGCATCTTCA ACAGCGCTGG AGTTGGTGCC GCCCACTTCC TGATCAAGGA GCTGTCTTAC 
CACAACCTGG AGCTGGAGCG GAACCGTCAG GAGGAGCTGG GAATCAAGCC CCAGGACATC 
TGGCCTTTCA TTGTGATTTC TGATGACTCC TGCGTGATGT GGAATGTGGT GGACGTCGAC 
AGTGGTGGGG AGAGAAGCAG AGAATTTTCC TGGTCGGAGA GAAACGTGTC CTTGAAGCAC 
ATCATGCAGC ACATCGAGGC ATCCCCCAAC ATCACCCACT ACGCCCTGAT TGGCATGCGG 
AAGTGGTCCA GCAAGACTGG GAGCCACGAG GTGCGAGAGC CCTTCTCCCG CTGCCATGTG 
CATGACTTCA TCGTCTTGAA CGTGGACTTG ACCCAGAACG TGCAGTACAA CCAGAACCGG 
TTCATGTGTG ACGACGTGGA CTTCAACCTG CGGGTGCACA GCGCCGGCCT CCTGCTCTGC 
CGGTTCAACC GCTTCAGCGT GATGAAGAAG CAGATCTCGG TGGGCGGGCA CAGGTCCTTC 
CACATCACGT CCAAGGTGTC GGACAGCCCC ATGGCCGTCG TGCCCGCCCA GTACATCTGC 
GCCCCCGACA GCAAGCACAC GTTCCTTGCG GCCCCCGCGC AGCTCCTCCT GGAGAAGTTC 
CTCCAGCACC ACAGCCACCG CTTCTTCCCG CTGTCCCTGA AAAATCTCGG CCACCCTGTG 
CTGTCCGTGG ACTGCTACCT GAACCTGGGG TCTCAGATCT CTGTGTGTTA TGTGAGCTCC 
AGGCCCCACT CTTTAAACAT CAGCTGCTCT GACTTCGTGT TCAGTGGACT CCTGCTGTAC 
CTCTGTGACT CTTTCGTGGG AGCTAGCTTT TTGAAAAAGT TTCATTTTCT GAAAGGTGCC 
ACCTTGTGTG TGATCTGTCA GGACCGCAAC TCGCTCCGCC AGACAGTCGT CCGCCTGGAG 
CTGGAAGACG AGTGGCAGTT TCGGCTGCGA GATGAATTCC AGACGGCCAA CGCCAAGGAG 
GACCGGCCAC TCTTCTTCCT GACCGGACGG CACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( NM_001205631.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192560.1 Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), mRNA.

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CloneID	OBa01629
Clone ID Related Accession (Same CDS sequence)	NM_001205631.1
Accession Version	NM_001205631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-10-23
Organism	Bos taurus(Cattle)
Product	protein GREB1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03017002.1, AAFC03074117.1 and AAFC03074120.1. On Apr 20, 2011 this sequence version replaced XM_584947.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192560.1
CDS	1..5832
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAEGRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	NM_001205631.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205631.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( NM_001205631.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192560.2 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	NM_001205631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	protein GREB1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On Jun 30, 2017 this sequence version replaced NM_001205631.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192560.2
CDS	1..5832
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	NM_001205631.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205631.2

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_010810181.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808483.1 Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X3, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_010810181.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808483.1
CDS	779..6610
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_010810181.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810181.2

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998445.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854213.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X8, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_024998445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854213.1
CDS	118..5949
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998445.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998445.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998446.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854214.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X9, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_024998446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854214.1
CDS	568..6399
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998446.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998446.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998447.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854215.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X10, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_024998447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854215.1
CDS	3930..9761
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998447.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998447.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998444.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854212.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X7, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_024998444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854212.1
CDS	273..6104
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998444.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998444.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_010810182.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808484.1 Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X1, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_010810182.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808484.1
CDS	1255..7086
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_010810182.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810182.2

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_024998443.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854211.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X6, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_024998443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854211.1
CDS	496..6327
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_024998443.1
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998443.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_005212947.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213004.1 Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X2, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_005212947.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213004.1
CDS	209..6040
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_005212947.3
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212947.3

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_010810181.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808483.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X3, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_010810181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808483.1
CDS	187..6018
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_010810181.3
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810181.3

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_005212947.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213004.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X4, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_005212947.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212947.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213004.1
CDS	75..5906
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_005212947.4
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212947.4

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_010810182.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808484.1 Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X5, mRNA.

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CloneID	OBa24163
Clone ID Related Accession (Same CDS sequence)	XM_010810181.2 , XM_024998445.1 , XM_024998446.1 , XM_024998447.1 , XM_024998444.1 , XM_010810182.2 , XM_024998443.1 , XM_005212947.3 , XM_010810181.3 , XM_005212947.4 , XM_010810182.3 , NM_001205631.2
Accession Version	XM_010810182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808484.1
CDS	94..5925
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_010810182.3
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810182.3

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTCCAC 
TTCATCATCC CCAAGTCCAA GGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GACTGCCCCT CGTCACAGAC AAGAGTCACG AATACATAAA GAGCCCGACG 
TTCACCCCCA CAACTGGCCG TCACGAGCAT GGACTCTTTA ACCTGTACCA CGCGATGGAT 
GGTGCCAGCC ACCTGCATGT GTTGGTTGTC AAGGAGTATG AGATGGCGAT TTATAAGAAG 
TACTGGCCCA ACCATATCAT GCTGGTCCTT CCCAGCATCT TCAACAGCGC TGGAGTTGGT 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAACC TGGAGCTGGA GCGGAACCGT 
CAGGAGGAGC TGGGAATCAA GCCCCAGGAC ATCTGGCCTT TCATTGTGAT TTCTGATGAC 
TCCTGCGTGA TGTGGAATGT GGTGGACGTC GACAGTGGTG GGGAGAGAAG CAGAGAATTT 
TCCTGGTCGG AGAGAAACGT GTCCTTGAAG CACATCATGC AGCACATCGA GGCATCCCCC 
AACATCACCC ACTACGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC TGGGAGCCAC 
GAGGTGCGAG AGCCCTTCTC CCGCTGCCAT GTGCATGACT TCATCGTCTT GAACGTGGAC 
TTGACCCAGA ACGTGCAGTA CAACCAGAAC CGGTTCATGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ACAGCGCCGG CCTCCTGCTC TGCCGGTTCA ACCGCTTCAG CGTGATGAAG 
AAGCAGATCT CGGTGGGCGG GCACAGGTCC TTCCACATCA CGTCCAAGGT GTCGGACAGC 
CCCATGGCCG TCGTGCCCGC CCAGTACATC TGCGCCCCCG ACAGCAAGCA CACGTTCCTT 
GCGGCCCCCG CGCAGCTCCT CCTGGAGAAG TTCCTCCAGC ACCACAGCCA CCGCTTCTTC 
CCGCTGTCCC TGAAAAATCT CGGCCACCCT GTGCTGTCCG TGGACTGCTA CCTGAACCTG 
GGGTCTCAGA TCTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCTTTAAA CATCAGCTGC 
TCTGACTTCG TGTTCAGTGG ACTCCTGCTG TACCTCTGTG ACTCTTTCGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCTTGT GTGTGATCTG TCAGGACCGC 
AACTCGCTCC GCCAGACAGT CGTCCGCCTG GAGCTGGAAG ACGAGTGGCA GTTTCGGCTG 
CGAGATGAAT TCCAGACGGC CAACGCCAAG GAGGACCGGC CACTCTTCTT CCTGACCGGA 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_010810180.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808482.1 Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X4, mRNA.

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CloneID	OBa47083
Clone ID Related Accession (Same CDS sequence)	XM_010810180.2
Accession Version	XM_010810180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGCGCTGTTC ATAACCTCTA CTCCCAAAGT GACCCGTCTG TGGGGCTGGT AGACAGATTA 
CTCAACTGCA AGGAGGTGAA GGAGGCCCCC AACATCGTGA CACTCCACGT GACCTCCTTC 
CCTTACGCGC TGCAGACGCA GCACACCCTC GTCAGCCCCT ACAACGAGAT CCACTGGCCG 
GCCTCCTACA GTGACGGCGT GGACTTGTAT CCTGAGAACA AGAAGTACTT CGGGCTGTCT 
GAGTTCATCG AGTCTACACT CTCAGGACAC AGCCTCCCAT TGCTGAGATA CGACAGCTCC 
TTTGAGGCCA TGGTCACCGC ACTGGGAAAA AGGTTCCCCC GCCTGCACAG CGCGGTGATC 
AGGACCTTTG TTCTCGTGCA GCACTACGCG GCCGCCATGA TGGCCGTGAG CGGCCTCTCG 
CAGATGAAGA ACTACACGTC CGTGGAGACG CTGGAGATCA CACAGAACCT CATCAACTCC 
CCGAAGCAGT GCCCCTGCGG CCACGGGCTC ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA 
CTGGCGGCCG TGGCCTATGA GCGGCTGGCC CACGTGCGGG CACGGCTGGC CCTGGAGGAG 
CACTTTGAGA TCATCCTGGG GAACCCCGCC TCGGGCATCA CCATCGGGAA GCACTTCCTG 
AAGCAGCTCA AGGTGTGGCA GAACATAGAG GACGCGGAGT GGCGGCCTCA GACGTACTTG 
GAGTTGGAGG GCCTGCCTTG CATCCTGATC TTCAGTGGGA TGGACCCACA CGGGGAGTCC 
TTACCAAGGT CCCTGAGGTA CTGTGACCTG CGGCTCATAA ACTCCTCCTG CCTGGTGAGG 
ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC GCGTACTTCG TGAGCAGTGA GGCTCCCCTG 
GAGAAGGGCG CCAGGACCGA GGCCTTGGAG AGTGATGCAG AGAAGCTGAG CAGCACAGAC 
AACGAGGACG AGGAGCTGGT GACAGAAGGC TCCACGTCAG AGAAGCGGAG CCCCGTGAAG 
AGAGAACGAT CCTGCTCCCA TGACTCCGCA TCTTCATCCC TCTCCTCTAA GGCATCCAGC 
TCGGCACCAT GTGGCGAGTC CTCGGCCGGA CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA 
CCATCCTGTG GCCCCTCGGA GGAGGCCAGG GCCCCTGGAG AGAGACAGAG GCTCCGGGCA 
GCGGGGGGGC CACCGTCGGT CATCAGCAGG CACAGCCCGG GGCTGACCCC ACAGCCGGAT 
CCCAGCCTCA AGAGCGGCCA GCACGTCCAG GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC 
TCGGCCTCCT CACCGGCACT GCCCGCCGCC GGCGCACTCG TCCTGCAGGC CTCCCAGTGC 
TCCATGACCA AGGCCTGCCG GCAGCCGCCC GTGGTCTTCC TGCCCAAGCT GGTGTACGAC 
ATGGTCACGT CCACGGATGG CAGCGGCCTG CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC 
TCGGTCATGT GGGCCAGCTC CTTCCGGCCC CTGCTCAGCA AGACCATGAC GTCCACGGAG 
CAGTCACTCT ACTACCGCCA GTGGACGGTG CCCCGGCCCG GCCACATGGA CTACGGCAAC 
CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC 
CAGATCGGGA AGACGGGTGC CTACCTGCAG TTCCTCAGCA TTTTGTCCAG AATGCTCATT 
CGGCTGACGG AAGTGGATGT TTACGATGAG GAAGAGATCA ACATCAACCT CAGAGAAGAT 
TCGGATTGGC ATTACCTCCA GCTCAGTGAC CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG 
TTGCCTTTTG ACTATATTAT TCATGACCCG AAGTATGAGG ACGCCAGTCT GATCTGCTCA 
CACCATCAGA GTGTAAAGAG TGAAGACAGA GGGACGCACC GGAAGCCGGA GGACCTGTAC 
GTGCGACGCC AGACGGCGAG GATGAGGCTC TCCAAGTTCG CAGCGTACAA CACGTACCAT 
CACTGCGAGC AGTGCCACCA GTACATGGGC TTCCACCCCC GCTACCAGCT CTATGAGTCC 
ACCCTGCACG CCTTTGCCTT CTCTTACTCC ATGCTAGGAG AGGAAATCCA GCTCCACTTC 
ATCATCCCCA AGTCCAAGGA GCACCACTTT GTCTTCAGCC AACCCGGAGG CCAGCTGGAG 
AGCATGCGAC TGCCCCTCGT CACAGACAAG AGTCACGAAT ACATAAAGAG CCCGACGTTC 
ACCCCCACAA CTGGCCGTCA CGAGCATGGA CTCTTTAACC TGTACCACGC GATGGATGGT 
GCCAGCCACC TGCATGTGTT GGTTGTCAAG GAGTATGAGA TGGCGATTTA TAAGAAGTAC 
TGGCCCAACC ATATCATGCT GGTCCTTCCC AGCATCTTCA ACAGCGCTGG AGTTGGTGCC 
GCCCACTTCC TGATCAAGGA GCTGTCTTAC CACAACCTGG AGCTGGAGCG GAACCGTCAG 
GAGGAGCTGG GAATCAAGCC CCAGGACATC TGGCCTTTCA TTGTGATTTC TGATGACTCC 
TGCGTGATGT GGAATGTGGT GGACGTCGAC AGTGGTGGGG AGAGAAGCAG AGAATTTTCC 
TGGTCGGAGA GAAACGTGTC CTTGAAGCAC ATCATGCAGC ACATCGAGGC ATCCCCCAAC 
ATCACCCACT ACGCCCTGAT TGGCATGCGG AAGTGGTCCA GCAAGACTGG GAGCCACGAG 
GTGCGAGAGC CCTTCTCCCG CTGCCATGTG CATGACTTCA TCGTCTTGAA CGTGGACTTG 
ACCCAGAACG TGCAGTACAA CCAGAACCGG TTCATGTGTG ACGACGTGGA CTTCAACCTG 
CGGGTGCACA GCGCCGGCCT CCTGCTCTGC CGGTTCAACC GCTTCAGCGT GATGAAGAAG 
CAGATCTCGG TGGGCGGGCA CAGGTCCTTC CACATCACGT CCAAGGTGTC GGACAGCCCC 
ATGGCCGTCG TGCCCGCCCA GTACATCTGC GCCCCCGACA GCAAGCACAC GTTCCTTGCG 
GCCCCCGCGC AGCTCCTCCT GGAGAAGTTC CTCCAGCACC ACAGCCACCG CTTCTTCCCG 
CTGTCCCTGA AAAATCTCGG CCACCCTGTG CTGTCCGTGG ACTGCTACCT GAACCTGGGG 
TCTCAGATCT CTGTGTGTTA TGTGAGCTCC AGGCCCCACT CTTTAAACAT CAGCTGCTCT 
GACTTCGTGT TCAGTGGACT CCTGCTGTAC CTCTGTGACT CTTTCGTGGG AGCTAGCTTT 
TTGAAAAAGT TTCATTTTCT GAAAGGTGCC ACCTTGTGTG TGATCTGTCA GGACCGCAAC 
TCGCTCCGCC AGACAGTCGT CCGCCTGGAG CTGGAAGACG AGTGGCAGTT TCGGCTGCGA 
GATGAATTCC AGACGGCCAA CGCCAAGGAG GACCGGCCAC TCTTCTTCCT GACCGGACGG 
CACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808482.1
CDS	1255..7083
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDSGGERSREFSWSERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSHEVREPFSRCHVHDFIVLNVDLTQNVQYNQNRFMCDDVDFNLRVHSAGLLLCRFNRFSVMKKQISVGGHRSFHITSKVSDSPMAVVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLKNLGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_010810180.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810180.2

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGCGCTGTTC ATAACCTCTA CTCCCAAAGT GACCCGTCTG TGGGGCTGGT AGACAGATTA 
CTCAACTGCA AGGAGGTGAA GGAGGCCCCC AACATCGTGA CACTCCACGT GACCTCCTTC 
CCTTACGCGC TGCAGACGCA GCACACCCTC GTCAGCCCCT ACAACGAGAT CCACTGGCCG 
GCCTCCTACA GTGACGGCGT GGACTTGTAT CCTGAGAACA AGAAGTACTT CGGGCTGTCT 
GAGTTCATCG AGTCTACACT CTCAGGACAC AGCCTCCCAT TGCTGAGATA CGACAGCTCC 
TTTGAGGCCA TGGTCACCGC ACTGGGAAAA AGGTTCCCCC GCCTGCACAG CGCGGTGATC 
AGGACCTTTG TTCTCGTGCA GCACTACGCG GCCGCCATGA TGGCCGTGAG CGGCCTCTCG 
CAGATGAAGA ACTACACGTC CGTGGAGACG CTGGAGATCA CACAGAACCT CATCAACTCC 
CCGAAGCAGT GCCCCTGCGG CCACGGGCTC ATGGTCCTGC TGCGGGTGCC CTGCTCCCCA 
CTGGCGGCCG TGGCCTATGA GCGGCTGGCC CACGTGCGGG CACGGCTGGC CCTGGAGGAG 
CACTTTGAGA TCATCCTGGG GAACCCCGCC TCGGGCATCA CCATCGGGAA GCACTTCCTG 
AAGCAGCTCA AGGTGTGGCA GAACATAGAG GACGCGGAGT GGCGGCCTCA GACGTACTTG 
GAGTTGGAGG GCCTGCCTTG CATCCTGATC TTCAGTGGGA TGGACCCACA CGGGGAGTCC 
TTACCAAGGT CCCTGAGGTA CTGTGACCTG CGGCTCATAA ACTCCTCCTG CCTGGTGAGG 
ACGGCCCTGG AGCAGGAGCT GGGCCTGGCC GCGTACTTCG TGAGCAGTGA GGCTCCCCTG 
GAGAAGGGCG CCAGGACCGA GGCCTTGGAG AGTGATGCAG AGAAGCTGAG CAGCACAGAC 
AACGAGGACG AGGAGCTGGT GACAGAAGGC TCCACGTCAG AGAAGCGGAG CCCCGTGAAG 
AGAGAACGAT CCTGCTCCCA TGACTCCGCA TCTTCATCCC TCTCCTCTAA GGCATCCAGC 
TCGGCACCAT GTGGCGAGTC CTCGGCCGGA CCCTCGCAGG TGGAGCGGGC CCGGTCCCCA 
CCATCCTGTG GCCCCTCGGA GGAGGCCAGG GCCCCTGGAG AGAGACAGAG GCTCCGGGCA 
GCGGGGGGGC CACCGTCGGT CATCAGCAGG CACAGCCCGG GGCTGACCCC ACAGCCGGAT 
CCCAGCCTCA AGAGCGGCCA GCACGTCCAG GTGTCGGCCC CGTCGGCGTC CTCCTCGGGC 
TCGGCCTCCT CACCGGCACT GCCCGCCGCC GGCGCACTCG TCCTGCAGGC CTCCCAGTGC 
TCCATGACCA AGGCCTGCCG GCAGCCGCCC GTGGTCTTCC TGCCCAAGCT GGTGTACGAC 
ATGGTCACGT CCACGGATGG CAGCGGCCTG CCCAAGGCCG CCTCGCTCCT GCCCTCGCAC 
TCGGTCATGT GGGCCAGCTC CTTCCGGCCC CTGCTCAGCA AGACCATGAC GTCCACGGAG 
CAGTCACTCT ACTACCGCCA GTGGACGGTG CCCCGGCCCG GCCACATGGA CTACGGCAAC 
CGCGCCGAGA GCCGCGTGGA CGGCTTCCAC CCGCGCCGGC TCCTGCTCAG CGGGCCCCCC 
CAGATCGGGA AGACGGGTGC CTACCTGCAG TTCCTCAGCA TTTTGTCCAG AATGCTCATT 
CGGCTGACGG AAGTGGATGT TTACGATGAG GAAGAGATCA ACATCAACCT CAGAGAAGAT 
TCGGATTGGC ATTACCTCCA GCTCAGTGAC CCCTGGCCGG ACCTGGAGCT GTTCAAGAAG 
TTGCCTTTTG ACTATATTAT TCATGACCCG AAGTATGAGG ACGCCAGTCT GATCTGCTCA 
CACCATCAGA GTGTAAAGAG TGAAGACAGA GGGACGCACC GGAAGCCGGA GGACCTGTAC 
GTGCGACGCC AGACGGCGAG GATGAGGCTC TCCAAGTTCG CAGCGTACAA CACGTACCAT 
CACTGCGAGC AGTGCCACCA GTACATGGGC TTCCACCCCC GCTACCAGCT CTATGAGTCC 
ACCCTGCACG CCTTTGCCTT CTCTTACTCC ATGCTAGGAG AGGAAATCCA GCTCCACTTC 
ATCATCCCCA AGTCCAAGGA GCACCACTTT GTCTTCAGCC AACCCGGAGG CCAGCTGGAG 
AGCATGCGAC TGCCCCTCGT CACAGACAAG AGTCACGAAT ACATAAAGAG CCCGACGTTC 
ACCCCCACAA CTGGCCGTCA CGAGCATGGA CTCTTTAACC TGTACCACGC GATGGATGGT 
GCCAGCCACC TGCATGTGTT GGTTGTCAAG GAGTATGAGA TGGCGATTTA TAAGAAGTAC 
TGGCCCAACC ATATCATGCT GGTCCTTCCC AGCATCTTCA ACAGCGCTGG AGTTGGTGCC 
GCCCACTTCC TGATCAAGGA GCTGTCTTAC CACAACCTGG AGCTGGAGCG GAACCGTCAG 
GAGGAGCTGG GAATCAAGCC CCAGGACATC TGGCCTTTCA TTGTGATTTC TGATGACTCC 
TGCGTGATGT GGAATGTGGT GGACGTCGAC AGTGGTGGGG AGAGAAGCAG AGAATTTTCC 
TGGTCGGAGA GAAACGTGTC CTTGAAGCAC ATCATGCAGC ACATCGAGGC ATCCCCCAAC 
ATCACCCACT ACGCCCTGAT TGGCATGCGG AAGTGGTCCA GCAAGACTGG GAGCCACGAG 
GTGCGAGAGC CCTTCTCCCG CTGCCATGTG CATGACTTCA TCGTCTTGAA CGTGGACTTG 
ACCCAGAACG TGCAGTACAA CCAGAACCGG TTCATGTGTG ACGACGTGGA CTTCAACCTG 
CGGGTGCACA GCGCCGGCCT CCTGCTCTGC CGGTTCAACC GCTTCAGCGT GATGAAGAAG 
CAGATCTCGG TGGGCGGGCA CAGGTCCTTC CACATCACGT CCAAGGTGTC GGACAGCCCC 
ATGGCCGTCG TGCCCGCCCA GTACATCTGC GCCCCCGACA GCAAGCACAC GTTCCTTGCG 
GCCCCCGCGC AGCTCCTCCT GGAGAAGTTC CTCCAGCACC ACAGCCACCG CTTCTTCCCG 
CTGTCCCTGA AAAATCTCGG CCACCCTGTG CTGTCCGTGG ACTGCTACCT GAACCTGGGG 
TCTCAGATCT CTGTGTGTTA TGTGAGCTCC AGGCCCCACT CTTTAAACAT CAGCTGCTCT 
GACTTCGTGT TCAGTGGACT CCTGCTGTAC CTCTGTGACT CTTTCGTGGG AGCTAGCTTT 
TTGAAAAAGT TTCATTTTCT GAAAGGTGCC ACCTTGTGTG TGATCTGTCA GGACCGCAAC 
TCGCTCCGCC AGACAGTCGT CCGCCTGGAG CTGGAAGACG AGTGGCAGTT TCGGCTGCGA 
GATGAATTCC AGACGGCCAA CGCCAAGGAG GACCGGCCAC TCTTCTTCCT GACCGGACGG 
CACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_010810188.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808490.1 Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X5, mRNA.

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CloneID	OBa47084
Clone ID Related Accession (Same CDS sequence)	XM_010810188.2
Accession Version	XM_010810188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GGAGAGGAAA 
TCCAGCTCCA CTTCATCATC CCCAAGTCCA AGGAGCACCA CTTTGTCTTC AGCCAACCCG 
GAGGCCAGCT GGAGAGCATG CGACTGCCCC TCGTCACAGA CAAGAGTCAC GAATACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808490.1
CDS	1259..5818
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPIFSQLYLEAEQQLSALEGGSRVDNEEEEEEGDGGLEQNCPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDIPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGALTKGPLICWKGSEFRSRQASMGACSSSLFPPLDSSGPPAPFPGEPGPGTAPSAPLGAPQAGPASEHPALTAALGLAVFNGKDSPKHQPLVKNRLSAVPRPSALGILSNSGPPKKRHKGWSPESPSASDGGYPQGGGHRAKYESAGVTCMPQVGLVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSISRAYEQYGASTIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAPPQPFPPVPGAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLGAFFSTLCPEGDIDILLDKFHQENQGLVSSSLTASSVTKSASLDVSGAPACTSYHLEPHRIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLLELGLEKEHVTRQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPSVGLVDRLLNCKEVKEAPNIVTLHVTSFPYALQTQHTLVSPYNEIHWPASYSDGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPASGITIGKHFLKQLKVWQNIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSSEAPLEKGARTEALESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERSCSHDSASSSLSSKASSSAPCGESSAGPSQVERARSPPSCGPSEEARAPGERQRLRAAGGPPSVISRHSPGLTPQPDPSLKSGQHVQVSAPSASSSGSASSPALPAAGALVLQASQCSMTKACRQPPVVFLPKLVYDMVTSTDGSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPGHMDYGNRAESRVDGFHPRRLLLSGPPQIGKTGAYLQFLSILSRMLIRLTEVDVYDEEEININLREDSDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHHQSVKSEDRGTHRKPEDLYVRRQTARMRLSKFAAYNTYHHCEQCHQYMGFHPRYQERKSSSTSSSPSPRSTTLSSANPEASWRACDCPSSQTRVTNT

Target ORF information:

RefSeq Version	XM_010810188.2
Organism	Bos taurus(cattle)
Definition	Bos taurus growth regulation by estrogen in breast cancer 1 (GREB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810188.2

ORF Insert Sequence:

ATGGGAAACT CGTACGCTGG ACAGCTGAAG ACCACGCGGT TCGAGGAGGT CCTGCACAAC 
TCCATCGAGG CCTCCCTGCG CTCCAACAGC CTGGTACCCA GGCCCATCTT CTCCCAGTTG 
TACCTAGAAG CAGAGCAGCA GCTTTCCGCT CTAGAAGGTG GAAGCCGAGT GGACAATGAA 
GAAGAGGAAG AGGAAGGGGA CGGGGGGCTG GAACAGAACT GTCCCCCAAA CCCCTACCAG 
ATGCACCCCC CGCCTGAAGG ATGCTGCACC ACGGACGGGT TCTGCCAGGC CGGGAAGGAC 
CTGCGTCTGG TCTCCATCTC CAGTGAGCCC ATCGACATCC CTGCGGGCTT CCTCCTGGTG 
GGTGCCAAGT CCCCCAGCCT GCCTGACCAT CTCCTGGTGT GTGCCGTGGA CAAGAGGTTC 
CTGCCGGATG ACAACGGACA CAACGCCCTT CTTGGTTTCT CTGGGAATTG TGTCGGCTGT 
GGCAAGAAGG GCTTCTGTTA CTTCACGGAG TTCTCCAATC ACATCAATCT GAAGCTGACC 
ACGCAGCCCA AGAAGCAGAA ACACTTGAAA TACTACCTGG TCCGCAACGC CCAGGGGGCT 
CTGACCAAAG GGCCTCTCAT TTGCTGGAAA GGCTCAGAGT TCAGAAGCCG GCAGGCATCC 
ATGGGTGCAT GCTCCAGTTC TCTGTTCCCG CCACTGGACA GCTCTGGGCC GCCGGCCCCC 
TTCCCTGGTG AGCCCGGTCC GGGGACAGCC CCGAGCGCCC CGCTGGGAGC CCCGCAGGCA 
GGACCAGCCT CCGAGCACCC CGCCCTAACC GCAGCCCTGG GTCTGGCTGT TTTCAACGGC 
AAAGATTCCC CGAAGCACCA GCCGCTGGTG AAGAACCGCC TGTCTGCAGT GCCGCGTCCC 
TCGGCCTTAG GTATCTTATC AAACTCTGGC CCTCCCAAAA AACGCCACAA AGGCTGGTCT 
CCAGAATCTC CATCAGCTTC GGATGGTGGC TACCCCCAGG GAGGGGGGCA CAGAGCCAAG 
TATGAAAGTG CAGGTGTGAC CTGCATGCCC CAGGTCGGCT TGGTGGGACC GGCTTCAGTC 
ACCTTTCCCG TTGTGGCTTC TGGAGAACCA GTGTCCGTTC CCGACAACTT GTTGAAGATA 
TGCAAGGCCA AGCCAGTGAT CTTTAAAGGC CACGGGAACT TCCCCTACCT CTGCGGCAAC 
CTGAACGACG TGGTTGTGAG CCCCCTGCTG TACACGTGCT ACCAGAACTC GCAGTCCATC 
TCCAGAGCCT ACGAGCAGTA CGGGGCCTCC ACCATTCAGC CCATCTCGGA GGAGATGCAG 
CTCCTGCTGA CCGTCTACTA TCTCGTCCAG CTGGCGGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGGTCGTGG CGTGAGTCGC ATCTAACGGA GATCCGGCAG 
TACCAGCAGG CGCCGCCCCA GCCCTTCCCA CCTGTGCCGG GTGCTGTGGC GCCGGTGACC 
TCCGCGCAGC TGCCCTGGCT GGCCGGCCTG GCTGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC TCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTGATC 
GAGGGCAAGC TGGCCAAGAC CAACTACGTG GTGATCATCT GCGCCTGCCG CAGCGCCGCC 
ATCGACTCCT GCATTGCTGT CACCGGAAAA TACCAAGCCC GGATTCTTTC TGAGAGCCTT 
CTCACCCCAG CTGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACTGG GAAAGTGGAC 
TCGCTAGGGG CCTTTTTTAG CACCCTCTGT CCAGAAGGCG ACATTGACAT TTTGCTGGAC 
AAATTCCATC AGGAAAATCA AGGCCTTGTT TCTTCCTCAC TTACTGCCTC CTCTGTCACC 
AAGTCAGCAT CCCTGGATGT CAGCGGGGCA CCTGCGTGCA CAAGTTACCA TCTGGAGCCG 
CACCGCATCC GGCCCTTCCA GCTGGCTGTG GCCCAGAAGC TCCTCTCCCA TGTGTGTTCA 
ATCGCGGATT CCAGCACCCA GAATCTGGAC TTGGGATCCT TTGAGAAGGT GGACTTTCTG 
ATTTGTATTC CACCCTCAGA AGTGACTTAC CAGCAGACCC TCTTCCATGT CTGGCATTCA 
GGGGTTTTGC TGGAGCTGGG CTTGGAGAAG GAGCACGTGA CCAGGCAGAG GGTGGAGCAA 
TATGTTCTGA AGCTTGATGC CGAAGCACAG ACGAAGTTCA AGTCCTTCCT GCAAAACTCC 
TTTCAGAATC CGCACACACT CTTCGTCCTC ATCCACGACC ATGCCCACTG GGATCTTGTG 
AGTAGCGCTG TTCATAACCT CTACTCCCAA AGTGACCCGT CTGTGGGGCT GGTAGACAGA 
TTACTCAACT GCAAGGAGGT GAAGGAGGCC CCCAACATCG TGACACTCCA CGTGACCTCC 
TTCCCTTACG CGCTGCAGAC GCAGCACACC CTCGTCAGCC CCTACAACGA GATCCACTGG 
CCGGCCTCCT ACAGTGACGG CGTGGACTTG TATCCTGAGA ACAAGAAGTA CTTCGGGCTG 
TCTGAGTTCA TCGAGTCTAC ACTCTCAGGA CACAGCCTCC CATTGCTGAG ATACGACAGC 
TCCTTTGAGG CCATGGTCAC CGCACTGGGA AAAAGGTTCC CCCGCCTGCA CAGCGCGGTG 
ATCAGGACCT TTGTTCTCGT GCAGCACTAC GCGGCCGCCA TGATGGCCGT GAGCGGCCTC 
TCGCAGATGA AGAACTACAC GTCCGTGGAG ACGCTGGAGA TCACACAGAA CCTCATCAAC 
TCCCCGAAGC AGTGCCCCTG CGGCCACGGG CTCATGGTCC TGCTGCGGGT GCCCTGCTCC 
CCACTGGCGG CCGTGGCCTA TGAGCGGCTG GCCCACGTGC GGGCACGGCT GGCCCTGGAG 
GAGCACTTTG AGATCATCCT GGGGAACCCC GCCTCGGGCA TCACCATCGG GAAGCACTTC 
CTGAAGCAGC TCAAGGTGTG GCAGAACATA GAGGACGCGG AGTGGCGGCC TCAGACGTAC 
TTGGAGTTGG AGGGCCTGCC TTGCATCCTG ATCTTCAGTG GGATGGACCC ACACGGGGAG 
TCCTTACCAA GGTCCCTGAG GTACTGTGAC CTGCGGCTCA TAAACTCCTC CTGCCTGGTG 
AGGACGGCCC TGGAGCAGGA GCTGGGCCTG GCCGCGTACT TCGTGAGCAG TGAGGCTCCC 
CTGGAGAAGG GCGCCAGGAC CGAGGCCTTG GAGAGTGATG CAGAGAAGCT GAGCAGCACA 
GACAACGAGG ACGAGGAGCT GGTGACAGAA GGCTCCACGT CAGAGAAGCG GAGCCCCGTG 
AAGAGAGAAC GATCCTGCTC CCATGACTCC GCATCTTCAT CCCTCTCCTC TAAGGCATCC 
AGCTCGGCAC CATGTGGCGA GTCCTCGGCC GGACCCTCGC AGGTGGAGCG GGCCCGGTCC 
CCACCATCCT GTGGCCCCTC GGAGGAGGCC AGGGCCCCTG GAGAGAGACA GAGGCTCCGG 
GCAGCGGGGG GGCCACCGTC GGTCATCAGC AGGCACAGCC CGGGGCTGAC CCCACAGCCG 
GATCCCAGCC TCAAGAGCGG CCAGCACGTC CAGGTGTCGG CCCCGTCGGC GTCCTCCTCG 
GGCTCGGCCT CCTCACCGGC ACTGCCCGCC GCCGGCGCAC TCGTCCTGCA GGCCTCCCAG 
TGCTCCATGA CCAAGGCCTG CCGGCAGCCG CCCGTGGTCT TCCTGCCCAA GCTGGTGTAC 
GACATGGTCA CGTCCACGGA TGGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCG 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGG CCCCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCAC TCTACTACCG CCAGTGGACG GTGCCCCGGC CCGGCCACAT GGACTACGGC 
AACCGCGCCG AGAGCCGCGT GGACGGCTTC CACCCGCGCC GGCTCCTGCT CAGCGGGCCC 
CCCCAGATCG GGAAGACGGG TGCCTACCTG CAGTTCCTCA GCATTTTGTC CAGAATGCTC 
ATTCGGCTGA CGGAAGTGGA TGTTTACGAT GAGGAAGAGA TCAACATCAA CCTCAGAGAA 
GATTCGGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CGGACCTGGA GCTGTTCAAG 
AAGTTGCCTT TTGACTATAT TATTCATGAC CCGAAGTATG AGGACGCCAG TCTGATCTGC 
TCACACCATC AGAGTGTAAA GAGTGAAGAC AGAGGGACGC ACCGGAAGCC GGAGGACCTG 
TACGTGCGAC GCCAGACGGC GAGGATGAGG CTCTCCAAGT TCGCAGCGTA CAACACGTAC 
CATCACTGCG AGCAGTGCCA CCAGTACATG GGCTTCCACC CCCGCTACCA GGAGAGGAAA 
TCCAGCTCCA CTTCATCATC CCCAAGTCCA AGGAGCACCA CTTTGTCTTC AGCCAACCCG 
GAGGCCAGCT GGAGAGCATG CGACTGCCCC TCGTCACAGA CAAGAGTCAC GAATACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GREB1
Entrez Gene ID	508204
Full Name	growth regulation by estrogen in breast cancer 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	GREB1	XP_001089374.1
Pan troglodytes (chimpanzee)	GREB1	XP_515304.3
Bos taurus (cattle)	GREB1	NP_001192560.1
Danio rerio (zebrafish)	greb1	XP_005158874.1
Homo sapiens (human)	GREB1	NP_055483.2
Canis lupus familiaris (dog)	GREB1	XP_005630186.1
Mus musculus (house mouse)	Greb1	NP_056579.2
Rattus norvegicus (Norway rat)	Greb1	XP_006239984.1
Gallus gallus (chicken)	GREB1	XP_004935825.1
Xenopus tropicalis (tropical clawed frog)	greb1	XP_002942230.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GREB1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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