ube2o cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ube2o gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube2o cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa47114 XM_018097883.1
Latest version!
Xenopus tropicalis ubiquitin conjugating enzyme E2O (ube2o), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa50268 XM_031894222.1
Latest version!
Xenopus tropicalis ubiquitin conjugating enzyme E2 O (ube2o), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa50269 XM_031894223.1
Latest version!
Xenopus tropicalis ubiquitin conjugating enzyme E2 O (ube2o), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa50270 XM_031894224.1
Latest version!
Xenopus tropicalis ubiquitin conjugating enzyme E2 O (ube2o), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa47114
    Clone ID Related Accession (Same CDS sequence) XM_018097883.1
    Accession Version XM_018097883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCTCGGACTC GGCTCCATCC TCCGCCTCCC CCGGGCCCCC GGCCGGTGAC 
    GGCTCCCAGC GGCTCCTGTT CTCTCACGAC TTGGTGTCCG GCTGCTACCG GGGCCACACC
    CGCTTCGGCC TGGTGCGTCT CATCCACGGA GAGGACTCGG AGGAAGAGGA GGATGCGCTG
    CCGGGGGAGG AGGAGAGCGG CCCCGGGGGG AGGAAGAAAG GAGCCGGCGG CGCTGCGGGA
    GGAGGGAGTC CCGGGTCGGA GCCGCCGCTC AGCAGGTTCG GCCCATTAAA GCGCGGATAT
    GTGCGGGTCC AGTGGTACCC GGAGGGCATC AAGCAGGACG TCAGGGAGAA CAAGTTAAAG
    CTGGAGGACC GCTCGCTGGT GCCCAGGGAT GTTGTGCGTC GGATGGGTAC AAAGGACAGC
    CAGTGCGGCA CGGTGATCGA CGTGAACATA GACTGCGTGG TGAAAGTGGT GGGGACCAAC
    TGCTTCCTGC ATCCGGTCAA CAGTAAGGAG CTGCACCACA TCTGGCCTAT CATGTACGGG
    GATTACATTG CCTACGACTG CTGGCTGGGC AAAGTGTATG ATCTGAAGAA CCAGGTTGTC
    CTCAAGCTGT CCAACGGCGC CAGATGTTCC ATGAGCACCG AGGATGCGTC CAAACTGTAC
    GACGTCTGCC CCCACGCCAG CGACTCGGGG CTGTTCTTTG ATGACTCCTA TGGCTTTTAC
    CCTGGGCAAG TACTGATTGG CCCCTCCAAG GTGTTCTCCA GCGTGCAGTG GCTCTCTGGG
    GTCAAGCCGG TACTGAGTGC CAAGAGCAAG TTTCGCGTGG GGGTGGAAGA GGTGCAAGTC
    ACTGAATTAC GAGTTACTTG GATCACAAAA AGCTTTTGTC TCGGCGGCAC CGATTCCATG
    GACCCCCCAC CCGCTGTGAT TACTCAGGAG AACCTGTCTA GAGTGAAACG ACTGGGCTCC
    TTCGACCACA CGCAGAGGCA GCTGGGAGAG CGCTGTCTGT ACATCTTCCC CACTAAGGTG
    GAACCCACCA AAATTCTGTG TGAAAGTCTC GAGAAGAACT GCCTGCTGGG GGAGGTCACC
    GTCACCAGGA AGGTCCATAC ACAGCAGGTG GAAAAGCTAC GGGAGAAGCA GATAGTCAAG
    ATAATCTCAT GTACCCAGGA TCCCTCCAAC CCTACTGAAC TCCTCAAAAA AAACTCCACA
    GTGCCCCCGT CGTATGAAAT ACCCACAGCA AGGGCAACCG AGGAAGATCT TGCCTCTGCC
    AAGCTGGAAG GGGAACCCCC CGAGGAACCG AACGTTCCGG AGTGCCCAAC CAAAGAGCAG
    CCTTTGGGCA CCTTCCGGGA AATATTCCAG GGCCTCCAGC TGCATTCCTC GGAACAGGAC
    GCCGACGATG AAGCCGCAGG GGATACCGAC GAGACCAGCT CGGTGACTTC CTCGGCCAGT
    TCTACGGCTT CTTCCCAAAG TGGCAGCGGC GCTGGGCGCA AGAAGAGCAT CCCCCTGTCC
    ATCCGGAACC TCAAGAGGAA ACATAAGAAG AAGAAAAAGA GCAAAGTATA CAGAGAGTTC
    AAACATGGCG ACAGGGTGGC TGTCGAGGTT ATAACCACTG TGACCACGGC TGACGTTATG
    TGGCAAGATG GGAGCGTTGA GACCAACATC CGCTCTAATG AACTGGTACC GATCCAGCAT
    CTGGATAACA ACGAGTTCTG CCCCGGGGAC TTTGTAGTCG ACAAGAGAGC CCACGCTGCC
    CAGGACCCCA GTATATACGG AGTGGTGCAG TCTGGGGATC ACACGGGCCG TACTTGTGTG
    GTAAAATGGA TCAAGCTGAA TTTGAATGGG GACGATACGG AGCTTGTTGG GGAAGAGGAG
    GATGTCAGTG TGTATGACAT TGTTGACCAT CCAGATTTCC GATTCCGAGC CACAGACATA
    GTGATCCGCA TTGGCAACTC TGATCCAGAA GCCTTTCCTG ATGCCAGCCA GCCGTCGGTG
    GGGCAGGTGG CTCGGGTGGA CGTGAGCAGC AAAGTCGAAG TGATCTGGGC TGATAATTCC
    CGGACTATTG TCCTCCCACA GCATCTGTAT AACGTGGAAT CCGAACTGGA GGAATCCTTT
    TATGACTCCG TGGACAGCAG CTCCAGCGGC GCCTCGTCTG ACGAATGGGA GGATGAGAGC
    GACAGCTGGG AAACGGACAA CGGGCTGACG GAAGATGAAA TCCCTCGGGC GGCGGGGGGG
    GAGGAGGCGG CGGCTTCACA GAATGAGACC GCCCCCGCAG AGCCGATGCA GGAACCGGCG
    CAGGACGAGG CGGAACAGAG CGCTGCATTG GCTGCGGCCG CAGATCTGGG AGTTCTCGGC
    GTGAAAGTGA ATGAGAAAGA AGGGATTGTG AAGAGCTTTA AGGAGCTGAA GGAGGCCATT
    AAGATCCTGG AGAGCCTGAA GAACATGACG GTAGAACAGC TGCTCACCGG CTCCCCAACC
    TCCCCCACAG CCGAGCCGGA GAAGTTCACT CGGGAGAAGA AGTTCCTGGA TGATATAAAG
    AAGCTGCAGG AGAACCTCAA GAAAACCCTG GACAATGTGG CCATAGCTGA GGAGGAAAAG
    ATGGAGTCGG CGCCCGAGAC TGAGAAGGAG GAGAAAGCTG AAGCGCAGAC GCCCGTCCGG
    TCAGAATGGC CGAGTGACAC GCCCGTGTTG TGCCAGCAGA GCGGCGGCAA ACCCGGCGTC
    ATCTTCACCA GCGCCAAAGG GGAGGTCTTC TCTGTGCTTG AAACTGCCCC CGAGAGCCAA
    GCCTTTAAGA AGCTGGAGTT CCAAGCCCCG GAAGCCAAGA AGTTCTTCAG CACCGTGCGT
    AAAGAGATGG CTCTGCTGGC GACTTCCCTC CCGGACGGCA TCATGGTGAA GACCTTTGAA
    GACCGAATGG TAAATACCAA TTTGTTTGCN NNNNNNNNNN NNGGGCCGAC GCGCACCCCG
    TATGAGGATG GGCTCTTCCT GTTCGACATG CAGCTCCCCA ATATCTACCC GGCGGTGCCC
    CCATTGTTCC GGTACCTGTC TCAGTGCAGC GGCAAGCTAA ACCCCAACCT CTATGACAAT
    GGGAAAGTGT GCGTCAGCCT CCTGGGGACC TGGATAGGAA AGGGTACCGA GAGGTGGACC
    AGCAAGTCCA GTCTCCTGCA GGTGCTAATC TCTATACAAG GCCTTATTTT AGTGCATGAG
    CCGTACTACA ACGAAGCGGG TTTCCACAGT GACCCAGGCT TGCAGGAAGG CTACCAAAAC
    AGCCGATGTT ACAACGAGAT GGCGCTTATC CGCGTGGTCC AATCCATGAC CCAAATGCTG
    CGGAGGCCGG TGGAAATCTT CCAACAGGAG ATCCGCGAGC ACTTTCAGAG CAGCGGTTGG
    CGCCTGGCGC ATCGCATAGA GAACTGGTTA GAAACCAATT CCATGGTAGA AAGAGGAGGC
    GAGCACAGGA ACGGGCAGGA GCACCCGTGT ATAGTGCCGT ACAGCCCGAC GGAGCCGGAG
    TGCACCCCGG AGCCAACTCT TGATTCCAAG GACCTAGATG ATTCTGGATG CGGTGCCTCC
    ACCATGTCCC CGGGGGAGTC CCAGCAATTC TCTGACTTGG AAAGCACAAG CAAAGGCGCC
    ACGGGGGGCG CCAGTCTCTC AGATGGGCCA AGAGGGGCGG CCGGGGGCGG GCAGCCCAGC
    GTCAAACCAA AGAAGCGCAG AAAGAGCTAT CGTAGTTTCC TCCCTGACAA ACGAGGCTAC
    CCCGATATCG GCTTCCCCCT CTTCCCCTTG TCCAAGGGCT TCATCAAGAG CATTCAGGGA
    GTTCTGGCTC AGTTCAAAGC TTCCCTGTTG GAAGCCGGCA TCTCGGAGCC TCGCGGAGAC
    AGACTGACGC TACCATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017953372.1
    CDS129..3989
    Translation

    Target ORF information:

    RefSeq Version XM_018097883.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin conjugating enzyme E2O (ube2o), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018097883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCTCGGACTC GGCTCCATCC TCCGCCTCCC CCGGGCCCCC GGCCGGTGAC 
    GGCTCCCAGC GGCTCCTGTT CTCTCACGAC TTGGTGTCCG GCTGCTACCG GGGCCACACC
    CGCTTCGGCC TGGTGCGTCT CATCCACGGA GAGGACTCGG AGGAAGAGGA GGATGCGCTG
    CCGGGGGAGG AGGAGAGCGG CCCCGGGGGG AGGAAGAAAG GAGCCGGCGG CGCTGCGGGA
    GGAGGGAGTC CCGGGTCGGA GCCGCCGCTC AGCAGGTTCG GCCCATTAAA GCGCGGATAT
    GTGCGGGTCC AGTGGTACCC GGAGGGCATC AAGCAGGACG TCAGGGAGAA CAAGTTAAAG
    CTGGAGGACC GCTCGCTGGT GCCCAGGGAT GTTGTGCGTC GGATGGGTAC AAAGGACAGC
    CAGTGCGGCA CGGTGATCGA CGTGAACATA GACTGCGTGG TGAAAGTGGT GGGGACCAAC
    TGCTTCCTGC ATCCGGTCAA CAGTAAGGAG CTGCACCACA TCTGGCCTAT CATGTACGGG
    GATTACATTG CCTACGACTG CTGGCTGGGC AAAGTGTATG ATCTGAAGAA CCAGGTTGTC
    CTCAAGCTGT CCAACGGCGC CAGATGTTCC ATGAGCACCG AGGATGCGTC CAAACTGTAC
    GACGTCTGCC CCCACGCCAG CGACTCGGGG CTGTTCTTTG ATGACTCCTA TGGCTTTTAC
    CCTGGGCAAG TACTGATTGG CCCCTCCAAG GTGTTCTCCA GCGTGCAGTG GCTCTCTGGG
    GTCAAGCCGG TACTGAGTGC CAAGAGCAAG TTTCGCGTGG GGGTGGAAGA GGTGCAAGTC
    ACTGAATTAC GAGTTACTTG GATCACAAAA AGCTTTTGTC TCGGCGGCAC CGATTCCATG
    GACCCCCCAC CCGCTGTGAT TACTCAGGAG AACCTGTCTA GAGTGAAACG ACTGGGCTCC
    TTCGACCACA CGCAGAGGCA GCTGGGAGAG CGCTGTCTGT ACATCTTCCC CACTAAGGTG
    GAACCCACCA AAATTCTGTG TGAAAGTCTC GAGAAGAACT GCCTGCTGGG GGAGGTCACC
    GTCACCAGGA AGGTCCATAC ACAGCAGGTG GAAAAGCTAC GGGAGAAGCA GATAGTCAAG
    ATAATCTCAT GTACCCAGGA TCCCTCCAAC CCTACTGAAC TCCTCAAAAA AAACTCCACA
    GTGCCCCCGT CGTATGAAAT ACCCACAGCA AGGGCAACCG AGGAAGATCT TGCCTCTGCC
    AAGCTGGAAG GGGAACCCCC CGAGGAACCG AACGTTCCGG AGTGCCCAAC CAAAGAGCAG
    CCTTTGGGCA CCTTCCGGGA AATATTCCAG GGCCTCCAGC TGCATTCCTC GGAACAGGAC
    GCCGACGATG AAGCCGCAGG GGATACCGAC GAGACCAGCT CGGTGACTTC CTCGGCCAGT
    TCTACGGCTT CTTCCCAAAG TGGCAGCGGC GCTGGGCGCA AGAAGAGCAT CCCCCTGTCC
    ATCCGGAACC TCAAGAGGAA ACATAAGAAG AAGAAAAAGA GCAAAGTATA CAGAGAGTTC
    AAACATGGCG ACAGGGTGGC TGTCGAGGTT ATAACCACTG TGACCACGGC TGACGTTATG
    TGGCAAGATG GGAGCGTTGA GACCAACATC CGCTCTAATG AACTGGTACC GATCCAGCAT
    CTGGATAACA ACGAGTTCTG CCCCGGGGAC TTTGTAGTCG ACAAGAGAGC CCACGCTGCC
    CAGGACCCCA GTATATACGG AGTGGTGCAG TCTGGGGATC ACACGGGCCG TACTTGTGTG
    GTAAAATGGA TCAAGCTGAA TTTGAATGGG GACGATACGG AGCTTGTTGG GGAAGAGGAG
    GATGTCAGTG TGTATGACAT TGTTGACCAT CCAGATTTCC GATTCCGAGC CACAGACATA
    GTGATCCGCA TTGGCAACTC TGATCCAGAA GCCTTTCCTG ATGCCAGCCA GCCGTCGGTG
    GGGCAGGTGG CTCGGGTGGA CGTGAGCAGC AAAGTCGAAG TGATCTGGGC TGATAATTCC
    CGGACTATTG TCCTCCCACA GCATCTGTAT AACGTGGAAT CCGAACTGGA GGAATCCTTT
    TATGACTCCG TGGACAGCAG CTCCAGCGGC GCCTCGTCTG ACGAATGGGA GGATGAGAGC
    GACAGCTGGG AAACGGACAA CGGGCTGACG GAAGATGAAA TCCCTCGGGC GGCGGGGGGG
    GAGGAGGCGG CGGCTTCACA GAATGAGACC GCCCCCGCAG AGCCGATGCA GGAACCGGCG
    CAGGACGAGG CGGAACAGAG CGCTGCATTG GCTGCGGCCG CAGATCTGGG AGTTCTCGGC
    GTGAAAGTGA ATGAGAAAGA AGGGATTGTG AAGAGCTTTA AGGAGCTGAA GGAGGCCATT
    AAGATCCTGG AGAGCCTGAA GAACATGACG GTAGAACAGC TGCTCACCGG CTCCCCAACC
    TCCCCCACAG CCGAGCCGGA GAAGTTCACT CGGGAGAAGA AGTTCCTGGA TGATATAAAG
    AAGCTGCAGG AGAACCTCAA GAAAACCCTG GACAATGTGG CCATAGCTGA GGAGGAAAAG
    ATGGAGTCGG CGCCCGAGAC TGAGAAGGAG GAGAAAGCTG AAGCGCAGAC GCCCGTCCGG
    TCAGAATGGC CGAGTGACAC GCCCGTGTTG TGCCAGCAGA GCGGCGGCAA ACCCGGCGTC
    ATCTTCACCA GCGCCAAAGG GGAGGTCTTC TCTGTGCTTG AAACTGCCCC CGAGAGCCAA
    GCCTTTAAGA AGCTGGAGTT CCAAGCCCCG GAAGCCAAGA AGTTCTTCAG CACCGTGCGT
    AAAGAGATGG CTCTGCTGGC GACTTCCCTC CCGGACGGCA TCATGGTGAA GACCTTTGAA
    GACCGAATGG TAAATACCAA TTTGTTTGCN NNNNNNNNNN NNGGGCCGAC GCGCACCCCG
    TATGAGGATG GGCTCTTCCT GTTCGACATG CAGCTCCCCA ATATCTACCC GGCGGTGCCC
    CCATTGTTCC GGTACCTGTC TCAGTGCAGC GGCAAGCTAA ACCCCAACCT CTATGACAAT
    GGGAAAGTGT GCGTCAGCCT CCTGGGGACC TGGATAGGAA AGGGTACCGA GAGGTGGACC
    AGCAAGTCCA GTCTCCTGCA GGTGCTAATC TCTATACAAG GCCTTATTTT AGTGCATGAG
    CCGTACTACA ACGAAGCGGG TTTCCACAGT GACCCAGGCT TGCAGGAAGG CTACCAAAAC
    AGCCGATGTT ACAACGAGAT GGCGCTTATC CGCGTGGTCC AATCCATGAC CCAAATGCTG
    CGGAGGCCGG TGGAAATCTT CCAACAGGAG ATCCGCGAGC ACTTTCAGAG CAGCGGTTGG
    CGCCTGGCGC ATCGCATAGA GAACTGGTTA GAAACCAATT CCATGGTAGA AAGAGGAGGC
    GAGCACAGGA ACGGGCAGGA GCACCCGTGT ATAGTGCCGT ACAGCCCGAC GGAGCCGGAG
    TGCACCCCGG AGCCAACTCT TGATTCCAAG GACCTAGATG ATTCTGGATG CGGTGCCTCC
    ACCATGTCCC CGGGGGAGTC CCAGCAATTC TCTGACTTGG AAAGCACAAG CAAAGGCGCC
    ACGGGGGGCG CCAGTCTCTC AGATGGGCCA AGAGGGGCGG CCGGGGGCGG GCAGCCCAGC
    GTCAAACCAA AGAAGCGCAG AAAGAGCTAT CGTAGTTTCC TCCCTGACAA ACGAGGCTAC
    CCCGATATCG GCTTCCCCCT CTTCCCCTTG TCCAAGGGCT TCATCAAGAG CATTCAGGGA
    GTTCTGGCTC AGTTCAAAGC TTCCCTGTTG GAAGCCGGCA TCTCGGAGCC TCGCGGAGAC
    AGACTGACGC TACCATTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50268
    Clone ID Related Accession (Same CDS sequence) XM_031894222.1
    Accession Version XM_031894222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCTCGGACTC GGCTCCATCC TCCGCCTCCC CCGGGCCCCC GGCCGGTGAC 
    GGCTCCCAGC GGCTCCTGTT CTCTCACGAC TTGGTGTCCG GCTGCTACCG GGGCCACACC
    CGCTTCGGCC TGGTGCGTCT CATCCACGGA GAGGACTCGG AGGAAGAGGA GGATGCGCTG
    CCGGGGGAGG AGGAGAGCGG CCCCGGGGGG AGGAAGAAAG GAGCCGGCGG CGCTGCGGGA
    GGAGGGAGTC CCGGGTCGGA GCCGCCGCTC AGCAGGTTCG GCCCATTAAA GCGCGGATAT
    GTGCGGGTCC AGTGGTACCC GGAGGGCATC AAGCAGGACG TCAGGGAGAA CAAGTTAAAG
    CTGGAGGACC GCTCGCTGGT GCCCAGGGAT GTTGTGCGTC GGATGGGTAC AAAGGACAGC
    CAGTGCGGCA CGGTGATCGA CGTGAACATA GACTGCGTGG TGAAAGTGGT GGGGACCAAC
    TGCTTCCTGC ATCCGGTCAA CAGTAAGGAG CTGCACCACA TCTGGCCTAT CATGTACGGG
    GATTACATTG CCTACGACTG CTGGCTGGGC AAAGTGTATG ATCTGAAGAA CCAGGTTGTC
    CTCAAGCTGT CCAACGGCGC CAGATGTTCC ATGAGCACCG AGGATGCGTC CAAACTGTAC
    GACGTCTGCC CCCACGCCAG CGACTCGGGG CTGTTCTTTG ATGACTCCTA TGGCTTTTAC
    CCTGGGCAAG TACTGATCGG CCCCTCCAAG GTGTTCTCCA GCGTGCAGTG GCTCTCTGGG
    GTCAAGCCGG TACTGAGTGC CAAGAGCAAG TTTCGCGTGG GGGTGGAAGA GGTGCAAGTC
    ACTGAATTAC GAGTTACTTG GATCACAAAA AGCTTTTGTC TCGGCGGCAC CGATTCCATG
    GACCCCCCAC CCGCTGTGAT TACTCAGGAG AACCTGTCTA GAGTGAAACG ACTGGGCTCC
    TTCGACCACA CGCAGAGGCA GCTGGGAGAG CGCTGTCTGT ACATCTTCCC CACTAAGGTG
    GAACCCACCA AAATTCTGTG TGAAAGTCTC GAGAAGAACT GCCTGCTGGG GGAGGTCACC
    GTCACCAGGA AGCAGGTGGA AAAGCTACGG GAGAAGCAGA TAGTCAAGAT AATCTCATGT
    ACCCAGGATC CCTCCAACCC TACTGAACTC CTCAAAAAAA ACTCCACAGT GCCCCCGTCG
    TATGAAATAC CCACAGCAAG GGCAACCGAG GAAGATCTTG TCTCTGCCAA GCTGGAAGGG
    GAACCCCCCG AGGAACCGAA CGTTCCGGAG TGCCCAACCA AAGAGCAGCC TTTGGGCACC
    TTCCGGGAAA TATTCCAGGG CCTCCAGCTG CATTCCTCGG AACAGGACGC CGACGATGAA
    GCCGCAGGGG ATACCGACGA GACCAGCTCG GTGACTTCCT CGGCCAGTTC TACGGCTTCT
    TCCCAAAGTG GCAGCGGCGC TGGGCGCAAG AAGAGCATCC CCCTGTCCAT CCGGAACCTC
    AAGAGGAAAC ATAAGAAGAA GAAAAAGAGC AAAGTATACA GAGAGTTCAA ACATGGCGAC
    AGGGTGGCTG TCGAGGTTAT AACCACTGTG ACCACGGCTG ACGTTATGTG GCAAGATGGG
    AGCGTTGAGA CCAACATCCG CTCTAATGAA CTGGTACCGA TCCAGCATCT GGATAACAAC
    GAGTTCTGCC CCGGGGACTT TGTAGTCGAC AAGAGAGCCC ACGCTGCCCA GGACCCCAGT
    ATATACGGAG TGGTGCAGTC TGGGGATCAC ACGGGCCGTA CTTGTGTGGT AAAATGGATC
    AAGCTGAATT TGAATGGGGA CGATACGGAG CTTGTTGGGG AAGAGGAGGA TGTCAGTGTG
    TATGACATTG TTGACCATCC AGATTTCCGA TTCCGAGCCA CAGACATAGT GATCCGCATT
    GGCAACTCTG ATCCAGAAGC CTTTCCTGAT GCCAGCCAGC CGTCGGTGGG GCAGGTGGCT
    CGGGTGGACG TGAGCAGCAA AGTCGAAGTG ATCTGGGCTG ATAATTCCCG GACTATTGTC
    CTCCCACAGC ATCTGTATAA CGTGGAATCC GAACTGGAGG AATCCTTTTA TGACTCCGTG
    GACAGCAGCT CCAGCGGCGC CTCGTCTGAT GAATGGGAGG ATGAGAGCGA CAGCTGGGAA
    ACGGACAACG GGCTGACGGA AGATGAAATC CCTCGGGCGG CGGGGGGGGA GGAGGCGGCG
    GCTTCACAGA ATGAGACCGC CCCCGCAGAG CCGATGCAGG AACCGGCGCA AGACGAGGCG
    GAACAGAGCG CTGCATTGGC TGCGGCCGCG GATCTGGGAG TTCTCGGCGT GAAAGTGAAT
    GAGAAAGAAG GGATTGTGAA GAGCTTTAAG GAGCTGAAGG AGGCCATTAA GATCCTGGAG
    AGCCTGAAGA ACATGACGGT AGAACAGCTG CTCACCGGCT CCCCAACCTC CCCCACAGCC
    GAGCCGGAGA AGTTCACTCG GGAGAAGAAG TTCCTGGATG ATATAAAGAA GCTGCAGGAG
    AACCTCAAGA AAACCCTGGA CAATGTGGCC ATAGCTGAGG AGGAAAAGAT GGAGTCGGCG
    CCCGAGACTG AGAAGGAGGA GAAAGCTGAA GCGCAGACGC CCGTCCGGTC AGAATGGCCG
    AGTGACACGC CCGTGTTGTG CCAGCAGAGC GGCGGCAAAC CCGGCGTCAT CTTCACCAGC
    GCCAAAGGGG AGGTCTTCTC TGTGCTTGAA ACTGCCCCCG AGAGCCAAGC CTTTAAGAAG
    CTGGAGTTCC AAGCCCCGGA AGCCAAGAAG TTCTTCAGCA CCGTGCGTAA AGAGATGGCT
    CTGCTGGCGA CTTCCCTCCC GGACGGCATC ATGGTGAAGA CCTTTGAAGA CCGAATGGAT
    CTGTTCTCTG CACTTATTAA AGGGCCGACG CGCACCCCGT ATGAGGATGG GCTCTTCCTG
    TTCGACATGC AGCTCCCCAA TATCTACCCG GCGGTGCCCC CATTGTTCCG GTACCTGTCT
    CAGTGCAGCG GCAAGCTAAA CCCCAACCTC TATGACAATG GGAAAGTGTG CGTCAGCCTC
    CTGGGGACCT GGATAGGAAA GGGTACCGAG AGGTGGACCA GCAAGTCCAG TCTCCTGCAG
    GTGCTAATCT CTATACAAGG CCTTATTTTA GTGAATGAGC CGTACTACAA CGAAGCGGGT
    TTCGACAGTG ACCGAGGCTT GCAGGAAGGC TACGAGAACA GCCGATGTTA CAACGAGATG
    GCGCTTATCC GCGTGGTCCA GTCCATGACG CAGATGCTGC GGAGGCCGGT GGAAGTCTTC
    CAACAGGAGA TCCGCGAGCA CTTTCAGAGC AGCGGTTGGC GCCTGGCGCA TCGCATAGAG
    AACTGGTTAG AAACCAATTC CATGGTAGAA AGAGGAGGCG AGCACAGGAA CGGGCAGGAG
    CACCCGTGTA TAGTGCCGTA CAGCCCGACG GAGCCGGAGT GCACCCCGGA GCCAACTCTT
    GATTCCAAGG ACCTAGATGA TTCTGGATGC GGTGCCTCCA CCATGTCCCC GGGGGAGTCC
    CAGCAATTCT CTGACTTGGA AAGCACAAGC AAAGGCGCCA CGGGGGGCGC CAGTCTCTCA
    GATGAGCCAA GAGGGGCGGC TGGGGGCGGG CAGCCCAGCG TCAAACCAAA GAAGCGCAGA
    AAGAGCTATC GTAGTTTCCT CCCTGACAAA CGAGGCTACC CCGATATCGG CTTCCCCCTC
    TTCCCCTTGT CCAAGGGCTT CATCAAGAGC ATTCAGGGAG TTCTGGCTCA GTTCAAAGCT
    TCCCTGTTGG AAGCCGGCAT CTCGGAGCCT CGCGGAGACA GACTGACGCT ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750082.1
    CDS128..3967
    Translation

    Target ORF information:

    RefSeq Version XM_031894222.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin conjugating enzyme E2 O (ube2o), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCTCGGACTC GGCTCCATCC TCCGCCTCCC CCGGGCCCCC GGCCGGTGAC 
    GGCTCCCAGC GGCTCCTGTT CTCTCACGAC TTGGTGTCCG GCTGCTACCG GGGCCACACC
    CGCTTCGGCC TGGTGCGTCT CATCCACGGA GAGGACTCGG AGGAAGAGGA GGATGCGCTG
    CCGGGGGAGG AGGAGAGCGG CCCCGGGGGG AGGAAGAAAG GAGCCGGCGG CGCTGCGGGA
    GGAGGGAGTC CCGGGTCGGA GCCGCCGCTC AGCAGGTTCG GCCCATTAAA GCGCGGATAT
    GTGCGGGTCC AGTGGTACCC GGAGGGCATC AAGCAGGACG TCAGGGAGAA CAAGTTAAAG
    CTGGAGGACC GCTCGCTGGT GCCCAGGGAT GTTGTGCGTC GGATGGGTAC AAAGGACAGC
    CAGTGCGGCA CGGTGATCGA CGTGAACATA GACTGCGTGG TGAAAGTGGT GGGGACCAAC
    TGCTTCCTGC ATCCGGTCAA CAGTAAGGAG CTGCACCACA TCTGGCCTAT CATGTACGGG
    GATTACATTG CCTACGACTG CTGGCTGGGC AAAGTGTATG ATCTGAAGAA CCAGGTTGTC
    CTCAAGCTGT CCAACGGCGC CAGATGTTCC ATGAGCACCG AGGATGCGTC CAAACTGTAC
    GACGTCTGCC CCCACGCCAG CGACTCGGGG CTGTTCTTTG ATGACTCCTA TGGCTTTTAC
    CCTGGGCAAG TACTGATCGG CCCCTCCAAG GTGTTCTCCA GCGTGCAGTG GCTCTCTGGG
    GTCAAGCCGG TACTGAGTGC CAAGAGCAAG TTTCGCGTGG GGGTGGAAGA GGTGCAAGTC
    ACTGAATTAC GAGTTACTTG GATCACAAAA AGCTTTTGTC TCGGCGGCAC CGATTCCATG
    GACCCCCCAC CCGCTGTGAT TACTCAGGAG AACCTGTCTA GAGTGAAACG ACTGGGCTCC
    TTCGACCACA CGCAGAGGCA GCTGGGAGAG CGCTGTCTGT ACATCTTCCC CACTAAGGTG
    GAACCCACCA AAATTCTGTG TGAAAGTCTC GAGAAGAACT GCCTGCTGGG GGAGGTCACC
    GTCACCAGGA AGCAGGTGGA AAAGCTACGG GAGAAGCAGA TAGTCAAGAT AATCTCATGT
    ACCCAGGATC CCTCCAACCC TACTGAACTC CTCAAAAAAA ACTCCACAGT GCCCCCGTCG
    TATGAAATAC CCACAGCAAG GGCAACCGAG GAAGATCTTG TCTCTGCCAA GCTGGAAGGG
    GAACCCCCCG AGGAACCGAA CGTTCCGGAG TGCCCAACCA AAGAGCAGCC TTTGGGCACC
    TTCCGGGAAA TATTCCAGGG CCTCCAGCTG CATTCCTCGG AACAGGACGC CGACGATGAA
    GCCGCAGGGG ATACCGACGA GACCAGCTCG GTGACTTCCT CGGCCAGTTC TACGGCTTCT
    TCCCAAAGTG GCAGCGGCGC TGGGCGCAAG AAGAGCATCC CCCTGTCCAT CCGGAACCTC
    AAGAGGAAAC ATAAGAAGAA GAAAAAGAGC AAAGTATACA GAGAGTTCAA ACATGGCGAC
    AGGGTGGCTG TCGAGGTTAT AACCACTGTG ACCACGGCTG ACGTTATGTG GCAAGATGGG
    AGCGTTGAGA CCAACATCCG CTCTAATGAA CTGGTACCGA TCCAGCATCT GGATAACAAC
    GAGTTCTGCC CCGGGGACTT TGTAGTCGAC AAGAGAGCCC ACGCTGCCCA GGACCCCAGT
    ATATACGGAG TGGTGCAGTC TGGGGATCAC ACGGGCCGTA CTTGTGTGGT AAAATGGATC
    AAGCTGAATT TGAATGGGGA CGATACGGAG CTTGTTGGGG AAGAGGAGGA TGTCAGTGTG
    TATGACATTG TTGACCATCC AGATTTCCGA TTCCGAGCCA CAGACATAGT GATCCGCATT
    GGCAACTCTG ATCCAGAAGC CTTTCCTGAT GCCAGCCAGC CGTCGGTGGG GCAGGTGGCT
    CGGGTGGACG TGAGCAGCAA AGTCGAAGTG ATCTGGGCTG ATAATTCCCG GACTATTGTC
    CTCCCACAGC ATCTGTATAA CGTGGAATCC GAACTGGAGG AATCCTTTTA TGACTCCGTG
    GACAGCAGCT CCAGCGGCGC CTCGTCTGAT GAATGGGAGG ATGAGAGCGA CAGCTGGGAA
    ACGGACAACG GGCTGACGGA AGATGAAATC CCTCGGGCGG CGGGGGGGGA GGAGGCGGCG
    GCTTCACAGA ATGAGACCGC CCCCGCAGAG CCGATGCAGG AACCGGCGCA AGACGAGGCG
    GAACAGAGCG CTGCATTGGC TGCGGCCGCG GATCTGGGAG TTCTCGGCGT GAAAGTGAAT
    GAGAAAGAAG GGATTGTGAA GAGCTTTAAG GAGCTGAAGG AGGCCATTAA GATCCTGGAG
    AGCCTGAAGA ACATGACGGT AGAACAGCTG CTCACCGGCT CCCCAACCTC CCCCACAGCC
    GAGCCGGAGA AGTTCACTCG GGAGAAGAAG TTCCTGGATG ATATAAAGAA GCTGCAGGAG
    AACCTCAAGA AAACCCTGGA CAATGTGGCC ATAGCTGAGG AGGAAAAGAT GGAGTCGGCG
    CCCGAGACTG AGAAGGAGGA GAAAGCTGAA GCGCAGACGC CCGTCCGGTC AGAATGGCCG
    AGTGACACGC CCGTGTTGTG CCAGCAGAGC GGCGGCAAAC CCGGCGTCAT CTTCACCAGC
    GCCAAAGGGG AGGTCTTCTC TGTGCTTGAA ACTGCCCCCG AGAGCCAAGC CTTTAAGAAG
    CTGGAGTTCC AAGCCCCGGA AGCCAAGAAG TTCTTCAGCA CCGTGCGTAA AGAGATGGCT
    CTGCTGGCGA CTTCCCTCCC GGACGGCATC ATGGTGAAGA CCTTTGAAGA CCGAATGGAT
    CTGTTCTCTG CACTTATTAA AGGGCCGACG CGCACCCCGT ATGAGGATGG GCTCTTCCTG
    TTCGACATGC AGCTCCCCAA TATCTACCCG GCGGTGCCCC CATTGTTCCG GTACCTGTCT
    CAGTGCAGCG GCAAGCTAAA CCCCAACCTC TATGACAATG GGAAAGTGTG CGTCAGCCTC
    CTGGGGACCT GGATAGGAAA GGGTACCGAG AGGTGGACCA GCAAGTCCAG TCTCCTGCAG
    GTGCTAATCT CTATACAAGG CCTTATTTTA GTGAATGAGC CGTACTACAA CGAAGCGGGT
    TTCGACAGTG ACCGAGGCTT GCAGGAAGGC TACGAGAACA GCCGATGTTA CAACGAGATG
    GCGCTTATCC GCGTGGTCCA GTCCATGACG CAGATGCTGC GGAGGCCGGT GGAAGTCTTC
    CAACAGGAGA TCCGCGAGCA CTTTCAGAGC AGCGGTTGGC GCCTGGCGCA TCGCATAGAG
    AACTGGTTAG AAACCAATTC CATGGTAGAA AGAGGAGGCG AGCACAGGAA CGGGCAGGAG
    CACCCGTGTA TAGTGCCGTA CAGCCCGACG GAGCCGGAGT GCACCCCGGA GCCAACTCTT
    GATTCCAAGG ACCTAGATGA TTCTGGATGC GGTGCCTCCA CCATGTCCCC GGGGGAGTCC
    CAGCAATTCT CTGACTTGGA AAGCACAAGC AAAGGCGCCA CGGGGGGCGC CAGTCTCTCA
    GATGAGCCAA GAGGGGCGGC TGGGGGCGGG CAGCCCAGCG TCAAACCAAA GAAGCGCAGA
    AAGAGCTATC GTAGTTTCCT CCCTGACAAA CGAGGCTACC CCGATATCGG CTTCCCCCTC
    TTCCCCTTGT CCAAGGGCTT CATCAAGAGC ATTCAGGGAG TTCTGGCTCA GTTCAAAGCT
    TCCCTGTTGG AAGCCGGCAT CTCGGAGCCT CGCGGAGACA GACTGACGCT ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50269
    Clone ID Related Accession (Same CDS sequence) XM_031894223.1
    Accession Version XM_031894223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTGCTGG GCTGGATAAA CCTCCCGCAA GCACCCCCAG CAAGTTCCAT TGGCAGCTTC 
    CAACAGCCAC AATCACGACT GTATGTAACG ACAATAACAA CAACAGTAAT AATGGATCCC
    AGATCCATGA TGATGGACTT TTATTCTTTG ATCTATGATT GGATTTCAGA ACCCAGTTTG
    GATAGTTCTG AGTTAAAGCT GGAGGACCGC TCGCTGGTGC CCAGGGATGT TGTGCGTCGG
    ATGGGTACAA AGGACAGCCA GTGCGGCACG GTGATCGACG TGAACATAGA CTGCGTGGTG
    AAAGTGGTGG GGACCAACTG CTTCCTGCAT CCGGTCAACA GTAAGGAGCT GCACCACATC
    TGGCCTATCA TGTACGGGGA TTACATTGCC TACGACTGCT GGCTGGGCAA AGTGTATGAT
    CTGAAGAACC AGGTTGTCCT CAAGCTGTCC AACGGCGCCA GATGTTCCAT GAGCACCGAG
    GATGCGTCCA AACTGTACGA CGTCTGCCCC CACGCCAGCG ACTCGGGGCT GTTCTTTGAT
    GACTCCTATG GCTTTTACCC TGGGCAAGTA CTGATCGGCC CCTCCAAGGT GTTCTCCAGC
    GTGCAGTGGC TCTCTGGGGT CAAGCCGGTA CTGAGTGCCA AGAGCAAGTT TCGCGTGGGG
    GTGGAAGAGG TGCAAGTCAC TGAATTACGA GTTACTTGGA TCACAAAAAG CTTTTGTCTC
    GGCGGCACCG ATTCCATGGA CCCCCCACCC GCTGTGATTA CTCAGGAGAA CCTGTCTAGA
    GTGAAACGAC TGGGCTCCTT CGACCACACG CAGAGGCAGC TGGGAGAGCG CTGTCTGTAC
    ATCTTCCCCA CTAAGGTGGA ACCCACCAAA ATTCTGTGTG AAAGTCTCGA GAAGAACTGC
    CTGCTGGGGG AGGTCACCGT CACCAGGAAG GTCCATACAC AGCAGGTGGA AAAGCTACGG
    GAGAAGCAGA TAGTCAAGAT AATCTCATGT ACCCAGGATC CCTCCAACCC TACTGAACTC
    CTCAAAAAAA ACTCCACAGT GCCCCCGTCG TATGAAATAC CCACAGCAAG GGCAACCGAG
    GAAGATCTTG TCTCTGCCAA GCTGGAAGGG GAACCCCCCG AGGAACCGAA CGTTCCGGAG
    TGCCCAACCA AAGAGCAGCC TTTGGGCACC TTCCGGGAAA TATTCCAGGG CCTCCAGCTG
    CATTCCTCGG AACAGGACGC CGACGATGAA GCCGCAGGGG ATACCGACGA GACCAGCTCG
    GTGACTTCCT CGGCCAGTTC TACGGCTTCT TCCCAAAGTG GCAGCGGCGC TGGGCGCAAG
    AAGAGCATCC CCCTGTCCAT CCGGAACCTC AAGAGGAAAC ATAAGAAGAA GAAAAAGAGC
    AAAGTATACA GAGAGTTCAA ACATGGCGAC AGGGTGGCTG TCGAGGTTAT AACCACTGTG
    ACCACGGCTG ACGTTATGTG GCAAGATGGG AGCGTTGAGA CCAACATCCG CTCTAATGAA
    CTGGTACCGA TCCAGCATCT GGATAACAAC GAGTTCTGCC CCGGGGACTT TGTAGTCGAC
    AAGAGAGCCC ACGCTGCCCA GGACCCCAGT ATATACGGAG TGGTGCAGTC TGGGGATCAC
    ACGGGCCGTA CTTGTGTGGT AAAATGGATC AAGCTGAATT TGAATGGGGA CGATACGGAG
    CTTGTTGGGG AAGAGGAGGA TGTCAGTGTG TATGACATTG TTGACCATCC AGATTTCCGA
    TTCCGAGCCA CAGACATAGT GATCCGCATT GGCAACTCTG ATCCAGAAGC CTTTCCTGAT
    GCCAGCCAGC CGTCGGTGGG GCAGGTGGCT CGGGTGGACG TGAGCAGCAA AGTCGAAGTG
    ATCTGGGCTG ATAATTCCCG GACTATTGTC CTCCCACAGC ATCTGTATAA CGTGGAATCC
    GAACTGGAGG AATCCTTTTA TGACTCCGTG GACAGCAGCT CCAGCGGCGC CTCGTCTGAT
    GAATGGGAGG ATGAGAGCGA CAGCTGGGAA ACGGACAACG GGCTGACGGA AGATGAAATC
    CCTCGGGCGG CGGGGGGGGA GGAGGCGGCG GCTTCACAGA ATGAGACCGC CCCCGCAGAG
    CCGATGCAGG AACCGGCGCA AGACGAGGCG GAACAGAGCG CTGCATTGGC TGCGGCCGCG
    GATCTGGGAG TTCTCGGCGT GAAAGTGAAT GAGAAAGAAG GGATTGTGAA GAGCTTTAAG
    GAGCTGAAGG AGGCCATTAA GATCCTGGAG AGCCTGAAGA ACATGACGGT AGAACAGCTG
    CTCACCGGCT CCCCAACCTC CCCCACAGCC GAGCCGGAGA AGTTCACTCG GGAGAAGAAG
    TTCCTGGATG ATATAAAGAA GCTGCAGGAG AACCTCAAGA AAACCCTGGA CAATGTGGCC
    ATAGCTGAGG AGGAAAAGAT GGAGTCGGCG CCCGAGACTG AGAAGGAGGA GAAAGCTGAA
    GCGCAGACGC CCGTCCGGTC AGAATGGCCG AGTGACACGC CCGTGTTGTG CCAGCAGAGC
    GGCGGCAAAC CCGGCGTCAT CTTCACCAGC GCCAAAGGGG AGGTCTTCTC TGTGCTTGAA
    ACTGCCCCCG AGAGCCAAGC CTTTAAGAAG CTGGAGTTCC AAGCCCCGGA AGCCAAGAAG
    TTCTTCAGCA CCGTGCGTAA AGAGATGGCT CTGCTGGCGA CTTCCCTCCC GGACGGCATC
    ATGGTGAAGA CCTTTGAAGA CCGAATGGAT CTGTTCTCTG CACTTATTAA AGGGCCGACG
    CGCACCCCGT ATGAGGATGG GCTCTTCCTG TTCGACATGC AGCTCCCCAA TATCTACCCG
    GCGGTGCCCC CATTGTTCCG GTACCTGTCT CAGTGCAGCG GCAAGCTAAA CCCCAACCTC
    TATGACAATG GGAAAGTGTG CGTCAGCCTC CTGGGGACCT GGATAGGAAA GGGTACCGAG
    AGGTGGACCA GCAAGTCCAG TCTCCTGCAG GTGCTAATCT CTATACAAGG CCTTATTTTA
    GTGAATGAGC CGTACTACAA CGAAGCGGGT TTCGACAGTG ACCGAGGCTT GCAGGAAGGC
    TACGAGAACA GCCGATGTTA CAACGAGATG GCGCTTATCC GCGTGGTCCA GTCCATGACG
    CAGATGCTGC GGAGGCCGGT GGAAGTCTTC CAACAGGAGA TCCGCGAGCA CTTTCAGAGC
    AGCGGTTGGC GCCTGGCGCA TCGCATAGAG AACTGGTTAG AAACCAATTC CATGGTAGAA
    AGAGGAGGCG AGCACAGGAA CGGGCAGGAG CACCCGTGTA TAGTGCCGTA CAGCCCGACG
    GAGCCGGAGT GCACCCCGGA GCCAACTCTT GATTCCAAGG ACCTAGATGA TTCTGGATGC
    GGTGCCTCCA CCATGTCCCC GGGGGAGTCC CAGCAATTCT CTGACTTGGA AAGCACAAGC
    AAAGGCGCCA CGGGGGGCGC CAGTCTCTCA GATGAGCCAA GAGGGGCGGC TGGGGGCGGG
    CAGCCCAGCG TCAAACCAAA GAAGCGCAGA AAGAGCTATC GTAGTTTCCT CCCTGACAAA
    CGAGGCTACC CCGATATCGG CTTCCCCCTC TTCCCCTTGT CCAAGGGCTT CATCAAGAGC
    ATTCAGGGAG TTCTGGCTCA GTTCAAAGCT TCCCTGTTGG AAGCCGGCAT CTCGGAGCCT
    CGCGGAGACA GACTGACGCT ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750083.1
    CDS33..3722
    Translation

    Target ORF information:

    RefSeq Version XM_031894223.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin conjugating enzyme E2 O (ube2o), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTGCTGG GCTGGATAAA CCTCCCGCAA GCACCCCCAG CAAGTTCCAT TGGCAGCTTC 
    CAACAGCCAC AATCACGACT GTATGTAACG ACAATAACAA CAACAGTAAT AATGGATCCC
    AGATCCATGA TGATGGACTT TTATTCTTTG ATCTATGATT GGATTTCAGA ACCCAGTTTG
    GATAGTTCTG AGTTAAAGCT GGAGGACCGC TCGCTGGTGC CCAGGGATGT TGTGCGTCGG
    ATGGGTACAA AGGACAGCCA GTGCGGCACG GTGATCGACG TGAACATAGA CTGCGTGGTG
    AAAGTGGTGG GGACCAACTG CTTCCTGCAT CCGGTCAACA GTAAGGAGCT GCACCACATC
    TGGCCTATCA TGTACGGGGA TTACATTGCC TACGACTGCT GGCTGGGCAA AGTGTATGAT
    CTGAAGAACC AGGTTGTCCT CAAGCTGTCC AACGGCGCCA GATGTTCCAT GAGCACCGAG
    GATGCGTCCA AACTGTACGA CGTCTGCCCC CACGCCAGCG ACTCGGGGCT GTTCTTTGAT
    GACTCCTATG GCTTTTACCC TGGGCAAGTA CTGATCGGCC CCTCCAAGGT GTTCTCCAGC
    GTGCAGTGGC TCTCTGGGGT CAAGCCGGTA CTGAGTGCCA AGAGCAAGTT TCGCGTGGGG
    GTGGAAGAGG TGCAAGTCAC TGAATTACGA GTTACTTGGA TCACAAAAAG CTTTTGTCTC
    GGCGGCACCG ATTCCATGGA CCCCCCACCC GCTGTGATTA CTCAGGAGAA CCTGTCTAGA
    GTGAAACGAC TGGGCTCCTT CGACCACACG CAGAGGCAGC TGGGAGAGCG CTGTCTGTAC
    ATCTTCCCCA CTAAGGTGGA ACCCACCAAA ATTCTGTGTG AAAGTCTCGA GAAGAACTGC
    CTGCTGGGGG AGGTCACCGT CACCAGGAAG GTCCATACAC AGCAGGTGGA AAAGCTACGG
    GAGAAGCAGA TAGTCAAGAT AATCTCATGT ACCCAGGATC CCTCCAACCC TACTGAACTC
    CTCAAAAAAA ACTCCACAGT GCCCCCGTCG TATGAAATAC CCACAGCAAG GGCAACCGAG
    GAAGATCTTG TCTCTGCCAA GCTGGAAGGG GAACCCCCCG AGGAACCGAA CGTTCCGGAG
    TGCCCAACCA AAGAGCAGCC TTTGGGCACC TTCCGGGAAA TATTCCAGGG CCTCCAGCTG
    CATTCCTCGG AACAGGACGC CGACGATGAA GCCGCAGGGG ATACCGACGA GACCAGCTCG
    GTGACTTCCT CGGCCAGTTC TACGGCTTCT TCCCAAAGTG GCAGCGGCGC TGGGCGCAAG
    AAGAGCATCC CCCTGTCCAT CCGGAACCTC AAGAGGAAAC ATAAGAAGAA GAAAAAGAGC
    AAAGTATACA GAGAGTTCAA ACATGGCGAC AGGGTGGCTG TCGAGGTTAT AACCACTGTG
    ACCACGGCTG ACGTTATGTG GCAAGATGGG AGCGTTGAGA CCAACATCCG CTCTAATGAA
    CTGGTACCGA TCCAGCATCT GGATAACAAC GAGTTCTGCC CCGGGGACTT TGTAGTCGAC
    AAGAGAGCCC ACGCTGCCCA GGACCCCAGT ATATACGGAG TGGTGCAGTC TGGGGATCAC
    ACGGGCCGTA CTTGTGTGGT AAAATGGATC AAGCTGAATT TGAATGGGGA CGATACGGAG
    CTTGTTGGGG AAGAGGAGGA TGTCAGTGTG TATGACATTG TTGACCATCC AGATTTCCGA
    TTCCGAGCCA CAGACATAGT GATCCGCATT GGCAACTCTG ATCCAGAAGC CTTTCCTGAT
    GCCAGCCAGC CGTCGGTGGG GCAGGTGGCT CGGGTGGACG TGAGCAGCAA AGTCGAAGTG
    ATCTGGGCTG ATAATTCCCG GACTATTGTC CTCCCACAGC ATCTGTATAA CGTGGAATCC
    GAACTGGAGG AATCCTTTTA TGACTCCGTG GACAGCAGCT CCAGCGGCGC CTCGTCTGAT
    GAATGGGAGG ATGAGAGCGA CAGCTGGGAA ACGGACAACG GGCTGACGGA AGATGAAATC
    CCTCGGGCGG CGGGGGGGGA GGAGGCGGCG GCTTCACAGA ATGAGACCGC CCCCGCAGAG
    CCGATGCAGG AACCGGCGCA AGACGAGGCG GAACAGAGCG CTGCATTGGC TGCGGCCGCG
    GATCTGGGAG TTCTCGGCGT GAAAGTGAAT GAGAAAGAAG GGATTGTGAA GAGCTTTAAG
    GAGCTGAAGG AGGCCATTAA GATCCTGGAG AGCCTGAAGA ACATGACGGT AGAACAGCTG
    CTCACCGGCT CCCCAACCTC CCCCACAGCC GAGCCGGAGA AGTTCACTCG GGAGAAGAAG
    TTCCTGGATG ATATAAAGAA GCTGCAGGAG AACCTCAAGA AAACCCTGGA CAATGTGGCC
    ATAGCTGAGG AGGAAAAGAT GGAGTCGGCG CCCGAGACTG AGAAGGAGGA GAAAGCTGAA
    GCGCAGACGC CCGTCCGGTC AGAATGGCCG AGTGACACGC CCGTGTTGTG CCAGCAGAGC
    GGCGGCAAAC CCGGCGTCAT CTTCACCAGC GCCAAAGGGG AGGTCTTCTC TGTGCTTGAA
    ACTGCCCCCG AGAGCCAAGC CTTTAAGAAG CTGGAGTTCC AAGCCCCGGA AGCCAAGAAG
    TTCTTCAGCA CCGTGCGTAA AGAGATGGCT CTGCTGGCGA CTTCCCTCCC GGACGGCATC
    ATGGTGAAGA CCTTTGAAGA CCGAATGGAT CTGTTCTCTG CACTTATTAA AGGGCCGACG
    CGCACCCCGT ATGAGGATGG GCTCTTCCTG TTCGACATGC AGCTCCCCAA TATCTACCCG
    GCGGTGCCCC CATTGTTCCG GTACCTGTCT CAGTGCAGCG GCAAGCTAAA CCCCAACCTC
    TATGACAATG GGAAAGTGTG CGTCAGCCTC CTGGGGACCT GGATAGGAAA GGGTACCGAG
    AGGTGGACCA GCAAGTCCAG TCTCCTGCAG GTGCTAATCT CTATACAAGG CCTTATTTTA
    GTGAATGAGC CGTACTACAA CGAAGCGGGT TTCGACAGTG ACCGAGGCTT GCAGGAAGGC
    TACGAGAACA GCCGATGTTA CAACGAGATG GCGCTTATCC GCGTGGTCCA GTCCATGACG
    CAGATGCTGC GGAGGCCGGT GGAAGTCTTC CAACAGGAGA TCCGCGAGCA CTTTCAGAGC
    AGCGGTTGGC GCCTGGCGCA TCGCATAGAG AACTGGTTAG AAACCAATTC CATGGTAGAA
    AGAGGAGGCG AGCACAGGAA CGGGCAGGAG CACCCGTGTA TAGTGCCGTA CAGCCCGACG
    GAGCCGGAGT GCACCCCGGA GCCAACTCTT GATTCCAAGG ACCTAGATGA TTCTGGATGC
    GGTGCCTCCA CCATGTCCCC GGGGGAGTCC CAGCAATTCT CTGACTTGGA AAGCACAAGC
    AAAGGCGCCA CGGGGGGCGC CAGTCTCTCA GATGAGCCAA GAGGGGCGGC TGGGGGCGGG
    CAGCCCAGCG TCAAACCAAA GAAGCGCAGA AAGAGCTATC GTAGTTTCCT CCCTGACAAA
    CGAGGCTACC CCGATATCGG CTTCCCCCTC TTCCCCTTGT CCAAGGGCTT CATCAAGAGC
    ATTCAGGGAG TTCTGGCTCA GTTCAAAGCT TCCCTGTTGG AAGCCGGCAT CTCGGAGCCT
    CGCGGAGACA GACTGACGCT ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50270
    Clone ID Related Accession (Same CDS sequence) XM_031894224.1
    Accession Version XM_031894224.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCTCGGACTC GGCTCCATCC TCCGCCTCCC CCGGGCCCCC GGCCGGTGAC 
    GGCTCCCAGC GGCTCCTGTT CTCTCACGAC TTGGTGTCCG GCTGCTACCG GGGCCACACC
    CGCTTCGGCC TGGTGCGTCT CATCCACGGA GAGGACTCGG AGGAAGAGGA GGATGCGCTG
    CCGGGGGAGG AGGAGAGCGG CCCCGGGGGG AGGAAGAAAG GAGCCGGCGG CGCTGCGGGA
    GGAGGGAGTC CCGGGTCGGA GCCGCCGCTC AGCAGGTTCG GCCCATTAAA GCGCGGATAT
    GTGCGGGTCC AGTGGTACCC GGAGGGCATC AAGCAGGACG TCAGGGAGAA CAAGTTAAAG
    CTGGAGGACC GCTCGCTGGT GCCCAGGGAT GTTGTGCGTC GGATGGGTAC AAAGGACAGC
    CAGTGCGGCA CGGTGATCGA CGTGAACATA GACTGCGTGG TGAAAGTGGT GGGGACCAAC
    TGCTTCCTGC ATCCGGTCAA CAGTAAGGAG CTGCACCACA TCTGGCCTAT CATGTACGGG
    GATTACATTG CCTACGACTG CTGGCTGGGC AAAGTGTATG ATCTGAAGAA CCAGGTTGTC
    CTCAAGCTGT CCAACGGCGC CAGATGTTCC ATGAGCACCG AGGATGCGTC CAAACTGTAC
    GACGTCTGCC CCCACGCCAG CGACTCGGGG CTGTTCTTTG ATGACTCCTA TGGCTTTTAC
    CCTGGGCAAG TACTGATCGG CCCCTCCAAG GTGTTCTCCA GCGTGCAGTG GCTCTCTGGG
    GTCAAGCCGG TACTGAGTGC CAAGAGCAAG TTTCGCGTGG GGGTGGAAGA GGTGCAAGTC
    ACTGAATTAC GAGTTACTTG GATCACAAAA AGCTTTTGTC TCGGCGGCAC CGATTCCATG
    GACCCCCCAC CCGCTGTGAT TACTCAGGAG AACCTGTCTA GAGTGAAACG ACTGGGCTCC
    TTCGACCACA CGCAGAGGCA GCTGGGAGAG CGCTGTCTGT ACATCTTCCC CACTAAGGTG
    GAACCCACCA AAATTCTGTG TGAAAGTCTC GAGAAGAACT GCCTGCTGGG GGAGGTCACC
    GTCACCAGGA AGGTCCATAC ACAGCAGGTG GAAAAGCTAC GGGAGAAGCA GATAGTCAAG
    ATAATCTCAT GTACCCAGGA TCCCTCCAAC CCTACTGAAC TCCTCAAAAA AAACTCCACA
    GTGCCCCCGT CGTATGAAAT ACCCACAGCA AGGGCAACCG AGGAAGATCT TGTCTCTGCC
    AAGCTGGAAG GGGAACCCCC CGAGGAACCG AACGTTCCGG AGTGCCCAAC CAAAGAGCAG
    CCTTTGGGCA CCTTCCGGGA AATATTCCAG GGCCTCCAGC TGCATTCCTC GGAACAGGAC
    GCCGACGATG AAGCCGCAGG GGATACCGAC GAGACCAGCT CGGTGACTTC CTCGGCCAGT
    TCTACGGCTT CTTCCCAAAG TGGCAGCGGC GCTGGGCGCA AGAAGAGCAT CCCCCTGTCC
    ATCCGGAACC TCAAGAGGAA ACATAAGAAG AAGAAAAAGA GCAAAGTATA CAGAGAGTTC
    AAACATGGCG ACAGGGTGGC TGTCGAGGTT ATAACCACTG TGACCACGGC TGACGTTATG
    TGGCAAGATG GGAGCGTTGA GACCAACATC CGCTCTAATG AACTGGTACC GATCCAGCAT
    CTGGATAACA ACGAGTTCTG CCCCGGGGAC TTTGTAGTCG ACAAGAGAGC CCACGCTGCC
    CAGGACCCCA GTATATACGG AGTGGTGCAG TCTGGGGATC ACACGGGCCG TACTTGTGTG
    GTAAAATGGA TCAAGCTGAA TTTGAATGGG GACGATACGG AGCTTGTTGG GGAAGAGGAG
    GATGTCAGTG TGTATGACAT TGTTGACCAT CCAGATTTCC GATTCCGAGC CACAGACATA
    GTGATCCGCA TTGGCAACTC TGATCCAGAA GCCTTTCCTG ATGCCAGCCA GCCGTCGGTG
    GGGCAGGTGG CTCGGGTGGA CGTGAGCAGC AAAGTCGAAG TGATCTGGGC TGATAATTCC
    CGGACTATTG TCCTCCCACA GCATCTGTAT AACGTGGAAT CCGAACTGGA GGAATCCTTT
    TATGACTCCG TGGACAGCAG CTCCAGCGGC GCCTCGTCTG ATGAATGGGA GGATGAGAGC
    GACAGCTGGG AAACGGACAA CGGGCTGACG GAAGATGAAA TCCCTCGGGC GGCGGGGGGG
    GAGGAGGCGG CGGCTTCACA GAATGAGACC GCCCCCGCAG AGCCGATGCA GGAACCGGCG
    CAAGACGAGG CGGAACAGAG CGCTGCATTG GCTGCGGCCG CGGATCTGGG AGTTCTCGGC
    GTGAAAGTGA ATGAGAAAGA AGGGATTGTG AAGAGCTTTA AGGAGCTGAA GGAGGCCATT
    AAGATCCTGG AGAGCCTGAA GAACATGACG GTAGAACAGC TGCTCACCGG CTCCCCAACC
    TCCCCCACAG CCGAGCCGGA GAAGTTCACT CGGGAGAAGA AGTTCCTGGA TGATATAAAG
    AAGCTGCAGG AGAACCTCAA GAAAACCCTG GACAATGTGG CCATAGCTGA GGAGGAAAAG
    ATGGAGTCGG CGCCCGAGAC TGAGAAGGAG GAGAAAGCTG AAGCGCAGAC GCCCGTCCGG
    TCAGAATGGC CGAGTGACAC GCCCGTGTTG TGCCAGCAGA GCGGCGGCAA ACCCGGCGTC
    ATCTTCACCA GCGCCAAAGG GGAGGTCTTC TCTGTGCTTG AAACTGCCCC CGAGAGCCAA
    GCCTTTAAGA AGCTGGAGTT CCAAGCCCCG GAAGCCAAGA AGTTCTTCAG CACCGTGCGT
    AAAGAGATGG CTCTGCTGGC GACTTCCCTC CCGGACGGCA TCATGGTGAA GACCTTTGAA
    GACCGAATGG ATCTGTTCTC TGCACTTATT AAAGGGCCGA CGCGCACCCC GTATGAGGAT
    GGGCTCTTCC TGTTCGACAT GCAGCTCCCC AATATCTACC CGGCGGTGCC CCCATTGTTC
    CGGTACCTGT CTCAGTGCAG CGGCAAGCTA AACCCCAACC TCTATGACAA TGGGAAAGTG
    TGCGTCAGCC TCCTGGGGAC CTGGATAGGA AAGGGTACCG AGAGGTGGAC CAGCAAGTCC
    AGTCTCCTGC AGGTGCTAAT CTCTATACAA GGCCTTATTT TAGTGAATGA GCCGTACTAC
    AACGAAGCGG GTTTCGACAG TGACCGAGGC TTGCAGGAAG GCTACGAGAA CAGCCGATGT
    TACAACGAGA TGGCGCTTAT CCGCGTGGTC CAGTCCATGA CGCAGATGCT GCGGAGGCCG
    GTGGAAGTCT TCCAACAGGA GATCCGCGAG CACTTTCAGA GCAGCGGTTG GCGCCTGGCG
    CATCGCATAG AGAACTGGTT AGAAACCAAT TCCATGGTAG AAAGAGGAGG CGAGCACAGG
    AACGGGCAGG AGCACCCGTG TATAGTGCCG TACAGCCCGA CGGAGCCGGA GTGCACCCCG
    GAGCCAACTC TTGATTCCAA GGACCTAGAT GATTCTGGAT GCGGTGCCTC CACCATGTCC
    CCGGGGGAGT CCCAGCAATT CTCTGACTTG GAAAGCACAA GCAAAGGCGC CACGGGGGGC
    GCCAGTCTCT CAGATGAGCC AAGAGGGGCG GCTGGGGGCG GGCAGCCCAG CGTCAAACCA
    AAGAAGCGCA GAAAGAGCTA TCGTAGTTTC CTCCCTGACA AACGAGGCTA CCCCGATATC
    GGCTTCCCCC TCTTCCCCTT GTCCAAGGGC TTCATCAAGA GCATTCAGGG AGTTCTGGCT
    CAGTTCAAAG CTTCCCTGTT GGAAGCCGGC ATCTCGGAGC CTCGCGGAGA CAGACTGACG
    CTACCATTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750084.1
    CDS172..4023
    Translation

    Target ORF information:

    RefSeq Version XM_031894224.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ubiquitin conjugating enzyme E2 O (ube2o), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCTCGGACTC GGCTCCATCC TCCGCCTCCC CCGGGCCCCC GGCCGGTGAC 
    GGCTCCCAGC GGCTCCTGTT CTCTCACGAC TTGGTGTCCG GCTGCTACCG GGGCCACACC
    CGCTTCGGCC TGGTGCGTCT CATCCACGGA GAGGACTCGG AGGAAGAGGA GGATGCGCTG
    CCGGGGGAGG AGGAGAGCGG CCCCGGGGGG AGGAAGAAAG GAGCCGGCGG CGCTGCGGGA
    GGAGGGAGTC CCGGGTCGGA GCCGCCGCTC AGCAGGTTCG GCCCATTAAA GCGCGGATAT
    GTGCGGGTCC AGTGGTACCC GGAGGGCATC AAGCAGGACG TCAGGGAGAA CAAGTTAAAG
    CTGGAGGACC GCTCGCTGGT GCCCAGGGAT GTTGTGCGTC GGATGGGTAC AAAGGACAGC
    CAGTGCGGCA CGGTGATCGA CGTGAACATA GACTGCGTGG TGAAAGTGGT GGGGACCAAC
    TGCTTCCTGC ATCCGGTCAA CAGTAAGGAG CTGCACCACA TCTGGCCTAT CATGTACGGG
    GATTACATTG CCTACGACTG CTGGCTGGGC AAAGTGTATG ATCTGAAGAA CCAGGTTGTC
    CTCAAGCTGT CCAACGGCGC CAGATGTTCC ATGAGCACCG AGGATGCGTC CAAACTGTAC
    GACGTCTGCC CCCACGCCAG CGACTCGGGG CTGTTCTTTG ATGACTCCTA TGGCTTTTAC
    CCTGGGCAAG TACTGATCGG CCCCTCCAAG GTGTTCTCCA GCGTGCAGTG GCTCTCTGGG
    GTCAAGCCGG TACTGAGTGC CAAGAGCAAG TTTCGCGTGG GGGTGGAAGA GGTGCAAGTC
    ACTGAATTAC GAGTTACTTG GATCACAAAA AGCTTTTGTC TCGGCGGCAC CGATTCCATG
    GACCCCCCAC CCGCTGTGAT TACTCAGGAG AACCTGTCTA GAGTGAAACG ACTGGGCTCC
    TTCGACCACA CGCAGAGGCA GCTGGGAGAG CGCTGTCTGT ACATCTTCCC CACTAAGGTG
    GAACCCACCA AAATTCTGTG TGAAAGTCTC GAGAAGAACT GCCTGCTGGG GGAGGTCACC
    GTCACCAGGA AGGTCCATAC ACAGCAGGTG GAAAAGCTAC GGGAGAAGCA GATAGTCAAG
    ATAATCTCAT GTACCCAGGA TCCCTCCAAC CCTACTGAAC TCCTCAAAAA AAACTCCACA
    GTGCCCCCGT CGTATGAAAT ACCCACAGCA AGGGCAACCG AGGAAGATCT TGTCTCTGCC
    AAGCTGGAAG GGGAACCCCC CGAGGAACCG AACGTTCCGG AGTGCCCAAC CAAAGAGCAG
    CCTTTGGGCA CCTTCCGGGA AATATTCCAG GGCCTCCAGC TGCATTCCTC GGAACAGGAC
    GCCGACGATG AAGCCGCAGG GGATACCGAC GAGACCAGCT CGGTGACTTC CTCGGCCAGT
    TCTACGGCTT CTTCCCAAAG TGGCAGCGGC GCTGGGCGCA AGAAGAGCAT CCCCCTGTCC
    ATCCGGAACC TCAAGAGGAA ACATAAGAAG AAGAAAAAGA GCAAAGTATA CAGAGAGTTC
    AAACATGGCG ACAGGGTGGC TGTCGAGGTT ATAACCACTG TGACCACGGC TGACGTTATG
    TGGCAAGATG GGAGCGTTGA GACCAACATC CGCTCTAATG AACTGGTACC GATCCAGCAT
    CTGGATAACA ACGAGTTCTG CCCCGGGGAC TTTGTAGTCG ACAAGAGAGC CCACGCTGCC
    CAGGACCCCA GTATATACGG AGTGGTGCAG TCTGGGGATC ACACGGGCCG TACTTGTGTG
    GTAAAATGGA TCAAGCTGAA TTTGAATGGG GACGATACGG AGCTTGTTGG GGAAGAGGAG
    GATGTCAGTG TGTATGACAT TGTTGACCAT CCAGATTTCC GATTCCGAGC CACAGACATA
    GTGATCCGCA TTGGCAACTC TGATCCAGAA GCCTTTCCTG ATGCCAGCCA GCCGTCGGTG
    GGGCAGGTGG CTCGGGTGGA CGTGAGCAGC AAAGTCGAAG TGATCTGGGC TGATAATTCC
    CGGACTATTG TCCTCCCACA GCATCTGTAT AACGTGGAAT CCGAACTGGA GGAATCCTTT
    TATGACTCCG TGGACAGCAG CTCCAGCGGC GCCTCGTCTG ATGAATGGGA GGATGAGAGC
    GACAGCTGGG AAACGGACAA CGGGCTGACG GAAGATGAAA TCCCTCGGGC GGCGGGGGGG
    GAGGAGGCGG CGGCTTCACA GAATGAGACC GCCCCCGCAG AGCCGATGCA GGAACCGGCG
    CAAGACGAGG CGGAACAGAG CGCTGCATTG GCTGCGGCCG CGGATCTGGG AGTTCTCGGC
    GTGAAAGTGA ATGAGAAAGA AGGGATTGTG AAGAGCTTTA AGGAGCTGAA GGAGGCCATT
    AAGATCCTGG AGAGCCTGAA GAACATGACG GTAGAACAGC TGCTCACCGG CTCCCCAACC
    TCCCCCACAG CCGAGCCGGA GAAGTTCACT CGGGAGAAGA AGTTCCTGGA TGATATAAAG
    AAGCTGCAGG AGAACCTCAA GAAAACCCTG GACAATGTGG CCATAGCTGA GGAGGAAAAG
    ATGGAGTCGG CGCCCGAGAC TGAGAAGGAG GAGAAAGCTG AAGCGCAGAC GCCCGTCCGG
    TCAGAATGGC CGAGTGACAC GCCCGTGTTG TGCCAGCAGA GCGGCGGCAA ACCCGGCGTC
    ATCTTCACCA GCGCCAAAGG GGAGGTCTTC TCTGTGCTTG AAACTGCCCC CGAGAGCCAA
    GCCTTTAAGA AGCTGGAGTT CCAAGCCCCG GAAGCCAAGA AGTTCTTCAG CACCGTGCGT
    AAAGAGATGG CTCTGCTGGC GACTTCCCTC CCGGACGGCA TCATGGTGAA GACCTTTGAA
    GACCGAATGG ATCTGTTCTC TGCACTTATT AAAGGGCCGA CGCGCACCCC GTATGAGGAT
    GGGCTCTTCC TGTTCGACAT GCAGCTCCCC AATATCTACC CGGCGGTGCC CCCATTGTTC
    CGGTACCTGT CTCAGTGCAG CGGCAAGCTA AACCCCAACC TCTATGACAA TGGGAAAGTG
    TGCGTCAGCC TCCTGGGGAC CTGGATAGGA AAGGGTACCG AGAGGTGGAC CAGCAAGTCC
    AGTCTCCTGC AGGTGCTAAT CTCTATACAA GGCCTTATTT TAGTGAATGA GCCGTACTAC
    AACGAAGCGG GTTTCGACAG TGACCGAGGC TTGCAGGAAG GCTACGAGAA CAGCCGATGT
    TACAACGAGA TGGCGCTTAT CCGCGTGGTC CAGTCCATGA CGCAGATGCT GCGGAGGCCG
    GTGGAAGTCT TCCAACAGGA GATCCGCGAG CACTTTCAGA GCAGCGGTTG GCGCCTGGCG
    CATCGCATAG AGAACTGGTT AGAAACCAAT TCCATGGTAG AAAGAGGAGG CGAGCACAGG
    AACGGGCAGG AGCACCCGTG TATAGTGCCG TACAGCCCGA CGGAGCCGGA GTGCACCCCG
    GAGCCAACTC TTGATTCCAA GGACCTAGAT GATTCTGGAT GCGGTGCCTC CACCATGTCC
    CCGGGGGAGT CCCAGCAATT CTCTGACTTG GAAAGCACAA GCAAAGGCGC CACGGGGGGC
    GCCAGTCTCT CAGATGAGCC AAGAGGGGCG GCTGGGGGCG GGCAGCCCAG CGTCAAACCA
    AAGAAGCGCA GAAAGAGCTA TCGTAGTTTC CTCCCTGACA AACGAGGCTA CCCCGATATC
    GGCTTCCCCC TCTTCCCCTT GTCCAAGGGC TTCATCAAGA GCATTCAGGG AGTTCTGGCT
    CAGTTCAAAG CTTCCCTGTT GGAAGCCGGC ATCTCGGAGC CTCGCGGAGA CAGACTGACG
    CTACCATTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.