UBE2O cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2O
Entrez Gene ID	789422
Full Name	ubiquitin conjugating enzyme E2 O
General protein information	Preferred Names ubiquitin conjugating enzyme E2 O Names (E3-independent) E2 ubiquitin-conjugating enzyme ubiquitin-conjugating enzyme E2O
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221305.4	XP_005221362.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
XM_024981049.1	XP_024836817.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
XM_015468274.1	XP_015323760.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
XM_005221305.3	XP_005221362.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
XM_002696167.4	XP_002696213.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
XM_015468273.1	XP_015323759.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
XM_024981050.1	XP_024836818.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
XM_002696167.5	XP_002696213.1	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2O	XP_001139963.2
Bos taurus (cattle)	UBE2O	XP_002696213.1
Gallus gallus (chicken)	UBE2O	XP_415619.4
Homo sapiens (human)	UBE2O	NP_071349.3
Rattus norvegicus (Norway rat)	Ube2o	XP_221132.5
	UBE2O	XP_001099166.2
Canis lupus familiaris (dog)	UBE2O	XP_540449.3
Mus musculus (house mouse)	Ube2o	NP_776116.2
Xenopus tropicalis (tropical clawed frog)	ube2o	XP_002936000.2

Gene Ontology

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227028	XM_002696167.5 Latest version!	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa227029	XM_005221305.4 Latest version!	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa227030	XM_024981049.1 Latest version!	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa227030	XM_024981050.1 Latest version!	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa30452	XM_015468273.1 Latest version!	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30452	XM_002696167.4 Latest version!	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30453	XM_015468274.1 Latest version!	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30453	XM_005221305.3 Latest version!	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2O ( XM_002696167.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696213.1 Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa227028
Clone ID Related Accession (Same CDS sequence)	XM_002696167.5
Accession Version	XM_002696167.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696167.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG 
CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG 
GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG 
GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA 
GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA 
GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT 
GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT 
TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC 
AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT 
GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC 
AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC 
TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC 
GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC 
CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC 
CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG 
GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG 
ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC 
GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG 
GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG 
GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG 
GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG 
TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA 
ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC 
TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG 
GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC 
GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG 
GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC 
ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGATTTCGGC 
CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC 
GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG 
CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC 
GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG 
CCCGAGAGCG CGGGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696213.1
CDS	79..3942
Translation	MADPAASAPAAPAPAQIPDVPPSPDAAPASAPAPASAADSGSGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSVAKKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_002696167.5
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696167.5

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG 
CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG 
GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG 
GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA 
GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA 
GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT 
GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT 
TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC 
AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT 
GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC 
AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC 
TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC 
GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC 
CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC 
CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG 
GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG 
ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC 
GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG 
GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG 
GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG 
GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG 
TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA 
ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC 
TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG 
GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC 
GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG 
GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC 
ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGATTTCGGC 
CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC 
GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG 
CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC 
GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG 
CCCGAGAGCG CGGGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_005221305.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221362.1 Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa227029
Clone ID Related Accession (Same CDS sequence)	XM_005221305.4
Accession Version	XM_005221305.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221305.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG 
CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC 
AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG 
GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC 
GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG 
GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC 
ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC 
AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC 
AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG 
GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC 
ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA 
GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG 
CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC 
GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT 
CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC 
GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC 
AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG 
TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT 
GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG 
GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA 
GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG 
GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG 
CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC 
ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA 
CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG 
ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG 
CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC 
CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC 
GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC 
CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC 
ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG 
GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC 
CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT 
GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC 
TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC 
TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC 
GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC 
CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG 
ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG 
GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC 
AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGATT TCGGCCGAGA GGAACCTGAG 
GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA 
GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC 
AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC 
TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG 
GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG 
GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221362.1
CDS	74..3862
Translation	MADPAASAPAAPAPAQIPDVPPSPDAAPASAPAPASAADSGSGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_005221305.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221305.4

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG 
CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC 
AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG 
GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC 
GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG 
GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC 
ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC 
AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC 
AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG 
GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC 
ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA 
GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG 
CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC 
GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT 
CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC 
GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC 
AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG 
TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT 
GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG 
GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA 
GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG 
GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG 
CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC 
ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA 
CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG 
ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG 
CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC 
CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC 
GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC 
CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC 
ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG 
GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC 
CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT 
GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC 
TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC 
TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC 
GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC 
CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG 
ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG 
GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC 
AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGATT TCGGCCGAGA GGAACCTGAG 
GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA 
GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC 
AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC 
TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG 
GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG 
GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_024981049.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836817.1 Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

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CloneID	OBa227030
Clone ID Related Accession (Same CDS sequence)	XM_024981049.1 , XM_024981050.1
Accession Version	XM_024981049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC 
TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC 
CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG 
GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT 
GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC 
GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG 
GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA 
GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG 
GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT 
GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC 
GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG 
AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA 
GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG 
CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG 
GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG 
GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC 
TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC 
ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA 
CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG 
GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT 
GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC 
CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC 
ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA 
GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA 
ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG 
CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT 
GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG 
GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA 
GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA 
GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG 
GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC 
GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG 
AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA 
GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA 
GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC 
GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG 
CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT 
GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC 
GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC 
TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG 
GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC 
ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC 
GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT 
GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG 
TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG 
AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC 
GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG 
CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT 
GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG 
GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG 
GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG 
GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG 
GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA 
AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC 
TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT 
GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836817.1
CDS	445..3849
Translation	MRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSVAKKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_024981049.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981049.1

ORF Insert Sequence:

ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC 
TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC 
CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG 
GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT 
GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC 
GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG 
GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA 
GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG 
GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT 
GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC 
GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG 
AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA 
GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG 
CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG 
GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG 
GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC 
TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC 
ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA 
CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG 
GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT 
GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC 
CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC 
ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA 
GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA 
ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG 
CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT 
GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG 
GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA 
GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA 
GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG 
GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC 
GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG 
AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA 
GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA 
GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC 
GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG 
CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT 
GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC 
GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC 
TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG 
GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC 
ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC 
GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT 
GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG 
TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG 
AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC 
GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG 
CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT 
GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG 
GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG 
GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG 
GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG 
GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA 
AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC 
TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT 
GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_024981050.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836818.1 Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa227030
Clone ID Related Accession (Same CDS sequence)	XM_024981049.1 , XM_024981050.1
Accession Version	XM_024981050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC 
TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC 
CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG 
GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT 
GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC 
GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG 
GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA 
GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG 
GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT 
GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC 
GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG 
AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA 
GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG 
CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG 
GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG 
GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC 
TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC 
ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA 
CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG 
GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT 
GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC 
CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC 
ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA 
GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA 
ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG 
CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT 
GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG 
GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA 
GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA 
GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG 
GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC 
GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG 
AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA 
GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA 
GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC 
GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG 
CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT 
GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC 
GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC 
TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG 
GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC 
ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC 
GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT 
GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG 
TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG 
AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC 
GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG 
CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT 
GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG 
GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG 
GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG 
GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG 
GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA 
AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC 
TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT 
GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836818.1
CDS	230..3634
Translation	MRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSVAKKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_024981050.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981050.1

ORF Insert Sequence:

ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC 
TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC 
CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG 
GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT 
GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC 
GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG 
GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA 
GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG 
GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT 
GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC 
GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG 
AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA 
GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG 
CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG 
GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG 
GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC 
TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC 
ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA 
CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG 
GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT 
GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC 
CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC 
ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA 
GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA 
ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG 
CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT 
GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG 
GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA 
GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA 
GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG 
GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC 
GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG 
AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA 
GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA 
GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC 
GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG 
CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT 
GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC 
GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC 
TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG 
GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC 
ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC 
GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT 
GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG 
TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG 
AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC 
GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG 
CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT 
GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG 
GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG 
GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG 
GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG 
GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA 
AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC 
TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT 
GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_002696167.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696213.1 Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30452
Clone ID Related Accession (Same CDS sequence)	XM_002696167.4 , XM_015468273.1
Accession Version	XM_002696167.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696167.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG 
CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG 
GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG 
GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA 
GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA 
GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT 
GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT 
TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC 
AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT 
GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC 
AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC 
TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC 
GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC 
CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC 
CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG 
GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG 
ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC 
GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG 
GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG 
GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG 
GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG 
TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA 
ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC 
TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG 
GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC 
GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG 
GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC 
ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC 
CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC 
GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG 
CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC 
GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG 
CCCGAGAGCG CGGGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696213.1
CDS	39..3902
Translation	MADPAASAPAAPAPAQIPDVPPSPDAAPASAPAPASAADSGSGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSVAKKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_002696167.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696167.4

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG 
CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG 
GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG 
GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA 
GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA 
GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT 
GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT 
TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC 
AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT 
GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC 
AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC 
TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC 
GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC 
CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC 
CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG 
GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG 
ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC 
GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG 
GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG 
GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG 
GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG 
TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA 
ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC 
TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG 
GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC 
GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG 
GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC 
ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC 
CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC 
GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG 
CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC 
GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG 
CCCGAGAGCG CGGGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_015468273.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323759.1 Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

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CloneID	OBa30452
Clone ID Related Accession (Same CDS sequence)	XM_002696167.4 , XM_015468273.1
Accession Version	XM_015468273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG 
CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG 
GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG 
GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA 
GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA 
GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT 
GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT 
TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC 
AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT 
GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC 
AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC 
TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC 
GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC 
CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC 
CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG 
GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG 
ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC 
GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG 
GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG 
GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG 
GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG 
TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA 
ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC 
TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG 
GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC 
GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG 
GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC 
ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC 
CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC 
GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG 
CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC 
GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG 
CCCGAGAGCG CGGGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323759.1
CDS	39..3902
Translation	MADPAASAPAAPAPAQIPDVPPSPDAAPASAPAPASAADSGSGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSVAKKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_015468273.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468273.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG 
CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG 
GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG 
GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA 
GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA 
GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC 
TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT 
GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC 
TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT 
TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC 
AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC 
TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC 
GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT 
GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC 
AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC 
TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC 
GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC 
CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC 
CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG 
GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG 
ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC 
GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC 
ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG 
GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG 
GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG 
GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG 
GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG 
GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG 
TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC 
ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG 
ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA 
ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC 
TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC 
CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC 
AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT 
CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG 
GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC 
GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG 
GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC 
ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG 
CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC 
CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG 
GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC 
GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG 
CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC 
GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG 
CCCGAGAGCG CGGGGGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_015468274.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323760.1 Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30453
Clone ID Related Accession (Same CDS sequence)	XM_015468274.1 , XM_005221305.3
Accession Version	XM_015468274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG 
CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC 
AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG 
GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC 
GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG 
GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC 
ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC 
AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC 
AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG 
GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC 
ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA 
GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG 
CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC 
GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT 
CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC 
GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC 
AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG 
TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT 
GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG 
GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA 
GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG 
GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG 
CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC 
ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA 
CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG 
ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG 
CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC 
CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC 
GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC 
CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC 
ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG 
GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC 
CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT 
GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC 
TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC 
TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC 
GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC 
CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG 
ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG 
GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC 
AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG 
GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA 
GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC 
AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC 
TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG 
GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG 
GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323760.1
CDS	39..3827
Translation	MADPAASAPAAPAPAQIPDVPPSPDAAPASAPAPASAADSGSGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_015468274.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468274.1

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG 
CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC 
AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG 
GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC 
GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG 
GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC 
ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC 
AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC 
AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG 
GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC 
ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA 
GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG 
CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC 
GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT 
CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC 
GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC 
AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG 
TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT 
GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG 
GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA 
GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG 
GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG 
CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC 
ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA 
CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG 
ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG 
CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC 
CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC 
GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC 
CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC 
ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG 
GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC 
CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT 
GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC 
TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC 
TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC 
GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC 
CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG 
ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG 
GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC 
AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG 
GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA 
GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC 
AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC 
TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG 
GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG 
GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_005221305.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221362.1 Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30453
Clone ID Related Accession (Same CDS sequence)	XM_015468274.1 , XM_005221305.3
Accession Version	XM_005221305.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG 
CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC 
AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG 
GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC 
GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG 
GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC 
ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC 
AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC 
AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG 
GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC 
ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA 
GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG 
CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC 
GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT 
CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC 
GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC 
AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG 
TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT 
GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG 
GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA 
GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG 
GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG 
CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC 
ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA 
CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG 
ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG 
CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC 
CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC 
GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC 
CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC 
ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG 
GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC 
CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT 
GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC 
TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC 
TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC 
GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC 
CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG 
ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG 
GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC 
AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG 
GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA 
GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC 
AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC 
TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG 
GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG 
GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221362.1
CDS	39..3827
Translation	MADPAASAPAAPAPAQIPDVPPSPDAAPASAPAPASAADSGSGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVKRLLKKQLVRITSCSPDVQCSRDHSMEDAGKKGAAKAKSETGSASPEETPDGSSSPVEMQDEGPEEAHEAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPTAEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTAEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPETERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGLPKDSSSPEPPAVAELSDFGREEPEDGGLAPGEASQGSDSEGPASAPRDRTEQASEAPPDASVPPSVKPKKRRKSYRSFLPEKSGYPDLGFPLFPLSKGFVKSIRGVLMQFRAALTEAGMPESAGDK

Target ORF information:

RefSeq Version	XM_005221305.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221305.3

ORF Insert Sequence:

ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG 
GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC 
CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC 
CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG 
GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG 
CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC 
GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG 
TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC 
ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC 
TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG 
AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC 
GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG 
GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC 
AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA 
GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC 
CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT 
GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG 
CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC 
AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG 
GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC 
GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG 
GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC 
ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC 
AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC 
AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG 
GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC 
ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA 
GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG 
CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC 
GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT 
CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC 
GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC 
AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG 
TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT 
GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG 
GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA 
GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG 
GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG 
CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC 
ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA 
CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG 
ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG 
CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC 
CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC 
GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC 
CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC 
ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG 
GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC 
CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT 
GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC 
TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC 
TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC 
GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC 
CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG 
ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG 
GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC 
AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG 
GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA 
GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC 
AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC 
TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG 
GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG 
GACAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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UBE2O cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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