UBE2O cDNA ORF clone, Homo sapiens(human)

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17561 NM_022066.4
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu50576 XM_005257567.1
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu71127 XM_024450865.1
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu71128 XM_024450866.1
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu71129 XM_024450867.1
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu87027 XM_017024934.1
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu87028 XM_017024935.1
Latest version!
Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OHu17561 NM_022066.3 Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17561
    Clone ID Related Accession (Same CDS sequence) NM_022066.3 , NM_022066.4
    Accession Version NM_022066.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB051521.1, DA092358.1 and BC025977.1. On Jun 24, 2008 this sequence version replaced NM_022066.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051521.1, SRR1803617.9431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC
    TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC
    TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA
    TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC
    AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG
    CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA
    GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC
    ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC
    AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA
    GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG
    GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC
    AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC
    CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG
    TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG
    AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC
    CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG
    GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC
    ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT
    TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC
    TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC
    CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA
    GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG
    AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC
    CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG
    ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA
    CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA
    GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT
    GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC
    TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT
    TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG
    GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT
    AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC
    CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC
    TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT
    GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC
    CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC
    GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC
    AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC
    GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC
    CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC
    GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC
    GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC
    TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG
    GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC
    TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA
    CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG
    CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071349.3
    CDS66..3944
    Translation

    Target ORF information:

    RefSeq Version NM_022066.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022066.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC
    TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC
    TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA
    TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC
    AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG
    CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA
    GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC
    ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC
    AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA
    GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG
    GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC
    AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC
    CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG
    TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG
    AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC
    CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG
    GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC
    ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT
    TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC
    TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC
    CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA
    GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG
    AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC
    CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG
    ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA
    CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA
    GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT
    GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC
    TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT
    TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG
    GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT
    AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC
    CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC
    TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT
    GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC
    CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC
    GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC
    AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC
    GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC
    CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC
    GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC
    GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC
    TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG
    GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC
    TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA
    CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG
    CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17561
    Clone ID Related Accession (Same CDS sequence) NM_022066.3 , NM_022066.4
    Accession Version NM_022066.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB051521.1, DA092358.1, BC025977.1 and AC090699.10. On Nov 23, 2018 this sequence version replaced NM_022066.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.9431.1, SRR1660809.204905.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000319380.12/ ENSP00000323687.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC
    TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC
    TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA
    TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC
    AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG
    CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA
    GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC
    ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC
    AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA
    GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG
    GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC
    AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC
    CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG
    TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG
    AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC
    CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG
    GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC
    ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT
    TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC
    TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC
    CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA
    GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG
    AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC
    CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG
    ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA
    CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA
    GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT
    GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC
    TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT
    TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG
    GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT
    AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC
    CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC
    TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT
    GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC
    CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC
    GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC
    AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC
    GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC
    CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC
    GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC
    GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC
    TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG
    GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC
    TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA
    CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG
    CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_071349.3
    CDS12..3890
    Translation

    Target ORF information:

    RefSeq Version NM_022066.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022066.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC
    TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC
    TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA
    TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC
    AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG
    CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA
    GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC
    ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC
    AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA
    GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG
    GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC
    AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC
    CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG
    TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG
    AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC
    CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCCATC GGTGGGCCAG
    GTGGCCCGTG TGGACGTCAG CAGCAAGGTG GAGGTGGTGT GGGCTGACAA CTCAAAGACC
    ATCATCCTGC CCCAGCACTT GTACAACATA GAGTCTGAGA TTGAGGAGTC AGACTACGAT
    TCGGTAGAAG GCAGCACCAG CGGGGCATCC TCGGATGAAT GGGAAGATGA TAGTGACAGC
    TGGGAGACGG ACAATGGGCT GGTGGAGGAC GAGCACCCCA AGATAGAGGA GCCCCCCATC
    CCACCCCTGG AGCAGCCGGT GGCCCCTGAG GACAAGGGAG TGGTGATCAG TGAAGAGGCA
    GCCACAGCTG CCGTCCAGGG GGCTGTGGCC ATGGCTGCCC CCATGGCCGG GCTGATGGAG
    AAGGCTGGCA AGGACGGGCC ACCCAAGAGC TTCCGGGAGT TGAAAGAGGC CATCAAGATC
    CTGGAGAGCC TCAAGAACAT GACTGTGGAG CAGCTGCTGA CGGGCTCGCC CACCTCTCCG
    ACTGTGGAGC CTGAGAAGCC AACTCGGGAG AAGAAGTTTC TGGATGACAT CAAGAAGCTA
    CAGGAAAACC TCAAGAAGAC CCTGGACAAT GTGGCCATTG TAGAGGAGGA GAAGATGGAA
    GCAGTGCCCG ACGTAGAGCG CAAGGAGGAC AAGCCCGAGG GGCAGTCACC TGTGAAGGCT
    GAGTGGCCCA GCGAAACCCC GGTGCTGTGC CAGCAGTGTG GCGGCAAGCC TGGCGTCACC
    TTCACCAGCG CCAAGGGCGA GGTCTTCTCC GTACTGGAGT TTGCACCCTC AAATCATTCT
    TTTAAGAAAA TTGAGTTCCA GCCTCCAGAA GCCAAGAAGT TCTTCAGCAC AGTGCGGAAG
    GAGATGGCGC TGCTGGCTAC CTCACTGCCT GAGGGCATCA TGGTCAAGAC TTTTGAAGAT
    AGAATGGACC TCTTCTCAGC TCTCATCAAG GGCCCCACTC GAACCCCCTA CGAGGATGGC
    CTCTACTTGT TTGACATCCA GCTCCCCAAC ATCTACCCAG CCGTGCCCCC CCACTTCTGC
    TACCTCTCCC AATGCAGTGG CCGCCTGAAC CCCAACCTGT ATGACAATGG GAAGGTGTGT
    GTCAGCCTCC TGGGCACCTG GATTGGAAAG GGGACAGAGA GGTGGACAAG CAAGTCCAGC
    CTTCTCCAGG TGCTCATCTC CATCCAAGGT CTGATCCTGG TAAATGAACC ATACTACAAC
    GAAGCCGGCT TCGACAGTGA CCGAGGCCTG CAGGAAGGCT ATGAAAACAG TCGCTGTTAC
    AATGAGATGG CGCTGATCCG CGTGGTGCAG TCCATGACCC AGCTGGTGCG GCGGCCCCCC
    GAGGTCTTTG AGCAGGAGAT CAGGCAACAC TTTAGCACTG GTGGCTGGCG GCTGGTGAAC
    CGTATCGAGT CCTGGCTGGA AACCCATGCC CTGCTGGAGA AGGCCCAGGC ACTGCCCAAC
    GGGGTGCCCA AGGCCAGCAG CTCGCCAGAG CCCCCAGCTG TAGCCGAGCT GTCAGACTCC
    GGCCAACAAG AACCTGAGGA TGGAGGGCCA GCCCCAGGAG AGGCCTCCCA GGGCTCAGAC
    TCAGAGGGCG GTGCCCAGGG CCTGGCCTCA GCTAGCAGGG ACCACACAGA CCAGACTTCG
    GAGACCGCAC CAGACGCATC GGTGCCACCC AGTGTGAAAC CAAAGAAGCG GAGAAAGAGC
    TACCGGAGCT TCTTACCTGA GAAGAGTGGC TACCCTGACA TCGGCTTCCC CCTCTTCCCA
    CTTTCCAAGG GTTTCATCAA GAGCATCCGG GGTGTCCTGA CGCAGTTCCG GGCTGCCCTG
    CTAGAGGCAG GCATGCCGGA GTGCACAGAG GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50576
    Clone ID Related Accession (Same CDS sequence) XM_005257567.1
    Accession Version XM_005257567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC
    TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC
    TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA
    TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC
    AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG
    CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA
    GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC
    ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC
    AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA
    GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG
    GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC
    AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC
    CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG
    TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG
    AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC
    CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCAGCC ATCGGTGGGC
    CAGGTGGCCC GTGTGGACGT CAGCAGCAAG GTGGAGGTGG TGTGGGCTGA CAACTCAAAG
    ACCATCATCC TGCCCCAGCA CTTGTACAAC ATAGAGTCTG AGATTGAGGA GTCAGACTAC
    GATTCGGTAG AAGGCAGCAC CAGCGGGGCA TCCTCGGATG AATGGGAAGA TGATAGTGAC
    AGCTGGGAGA CGGACAATGG GCTGGTGGAG GACGAGCACC CCAAGATAGA GGAGCCCCCC
    ATCCCACCCC TGGAGCAGCC GGTGGCCCCT GAGGACAAGG GAGTGGTGAT CAGTGAAGAG
    GCAGCCACAG CTGCCGTCCA GGGGGCTGTG GCCATGGCTG CCCCCATGGC CGGGCTGATG
    GAGAAGGCTG GCAAGGACGG GCCACCCAAG AGCTTCCGGG AGTTGAAAGA GGCCATCAAG
    ATCCTGGAGA GCCTCAAGAA CATGACTGTG GAGCAGCTGC TGACGGGCTC GCCCACCTCT
    CCGACTGTGG AGCCTGAGAA GCCAACTCGG GAGAAGAAGT TTCTGGATGA CATCAAGAAG
    CTACAGGAAA ACCTCAAGAA GACCCTGGAC AATGTGGCCA TTGTAGAGGA GGAGAAGATG
    GAAGCAGTGC CCGACGTAGA GCGCAAGGAG GACAAGCCCG AGGGGCAGTC ACCTGTGAAG
    GCTGAGTGGC CCAGCGAAAC CCCGGTGCTG TGCCAGCAGT GTGGCGGCAA GCCTGGCGTC
    ACCTTCACCA GCGCCAAGGG CGAGGTCTTC TCCGTACTGG AGTTTGCACC CTCAAATCAT
    TCTTTTAAGA AAATTGAGTT CCAGCCTCCA GAAGCCAAGA AGTTCTTCAG CACAGTGCGG
    AAGGAGATGG CGCTGCTGGC TACCTCACTG CCTGAGGGCA TCATGGTCAA GACTTTTGAA
    GATAGAATGG ACCTCTTCTC AGCTCTCATC AAGGGCCCCA CTCGAACCCC CTACGAGGAT
    GGCCTCTACT TGTTTGACAT CCAGCTCCCC AACATCTACC CAGCCGTGCC CCCCCACTTC
    TGCTACCTCT CCCAATGCAG TGGCCGCCTG AACCCCAACC TGTATGACAA TGGGAAGGTG
    TGTGTCAGCC TCCTGGGCAC CTGGATTGGA AAGGGGACAG AGAGGTGGAC AAGCAAGTCC
    AGCCTTCTCC AGGTGCTCAT CTCCATCCAA GGTCTGATCC TGGTAAATGA ACCATACTAC
    AACGAAGCCG GCTTCGACAG TGACCGAGGC CTGCAGGAAG GCTATGAAAA CAGTCGCTGT
    TACAATGAGA TGGCGCTGAT CCGCGTGGTG CAGTCCATGA CCCAGCTGGT GCGGCGGCCC
    CCCGAGGTCT TTGAGCAGGA GATCAGGCAA CACTTTAGCA CTGGTGGCTG GCGGCTGGTG
    AACCGTATCG AGTCCTGGCT GGAAACCCAT GCCCTGCTGG AGAAGGCCCA GGCACTGCCC
    AACGGGGTGC CCAAGGCCAG CAGCTCGCCA GAGCCCCCAG CTGTAGCCGA GCTGTCAGAC
    TCCGGCCAAC AAGAACCTGA GGATGGAGGG CCAGCCCCAG GAGAGGCCTC CCAGGGCTCA
    GACTCAGAGG GCGGTGCCCA GGGCCTGGCC TCAGCTAGCA GGGACCACAC AGACCAGACT
    TCGGAGACCG CACCAGACGC ATCGGTGCCA CCCAGTGTGA AACCAAAGAA GCGGAGAAAG
    AGCTACCGGA GCTTCTTACC TGAGAAGAGT GGCTACCCTG ACATCGGCTT CCCCCTCTTC
    CCACTTTCCA AGGGTTTCAT CAAGAGCATC CGGGGTGTCC TGACGCAGTT CCGGGCTGCC
    CTGCTAGAGG CAGGCATGCC GGAGTGCACA GAGGACAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005257624.1
    CDS50..3931
    Translation

    Target ORF information:

    RefSeq Version XM_005257567.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTAGAGCCAG CCAAGATTGC CTGGGAATGT CCAGAAAAAA ACTGCGCCCA GGGGGAGGGC
    TCTATGGCCA AGAAGGTGAA GCGCCTGTTG AAGAAGCAGG TTGTGCGGAT CATGTCATGC
    TCCCCAGACA CCCAGTGTTC CCGGGACCAT TCCATGGAAG ACCCAGACAA GAAGGGGGAA
    TCCAAAACCA AGAGCGAAGC GGAGTCTGCC AGCCCTGAGG AGACGCCCGA TGGCTCTGCC
    AGTCCAGTGG AGATGCAGGA CGAGGGTGCA GAGGAGCCCC ACGAGGCAGG AGAGCAGCTG
    CCCCCATTCC TGCTAAAAGA AGGCAGAGAT GACAGGCTGC ACTCGGCAGA GCAGGACGCA
    GATGATGAGG CTGCTGATGA CACGGACGAC ACCAGTTCGG TGACCTCCTC TGCCAGCTCC
    ACCACTTCCT CCCAGAGCGG CAGCGGCACG AGTCGCAAAA AGAGCATCCC CTTGTCCATC
    AAGAACTTAA AGCGCAAACA CAAGAGGAAG AAGAATAAAA TCACTCGAGA CTTCAAGCCA
    GGGGACAGGG TGGCAGTGGA GGTGGTGACC ACGATGACCT CAGCCGACGT GATGTGGCAG
    GATGGCTCCG TGGAATGCAA CATCCGCTCC AACGACCTCT TCCCTGTGCA CCACCTGGAC
    AACAACGAGT TCTGCCCTGG AGACTTCGTG GTAGATAAGC GAGTCCAGAG CTGTCCAGAC
    CCTGCTGTCT ACGGTGTGGT ACAGTCTGGG GACCACATCG GCCGTACCTG CATGGTGAAG
    TGGTTCAAGC TGAGGCCGAG TGGGGACGAC GTGGAGCTGA TTGGAGAAGA GGAAGATGTG
    AGTGTTTACG ACATTGCTGA CCACCCTGAC TTTAGGTTCC GTACAACTGA CATCGTCATC
    CGCATCGGCA ATACTGAGGA TGGGGCTCCT CACAAGGAGG ATGAGCAGCC ATCGGTGGGC
    CAGGTGGCCC GTGTGGACGT CAGCAGCAAG GTGGAGGTGG TGTGGGCTGA CAACTCAAAG
    ACCATCATCC TGCCCCAGCA CTTGTACAAC ATAGAGTCTG AGATTGAGGA GTCAGACTAC
    GATTCGGTAG AAGGCAGCAC CAGCGGGGCA TCCTCGGATG AATGGGAAGA TGATAGTGAC
    AGCTGGGAGA CGGACAATGG GCTGGTGGAG GACGAGCACC CCAAGATAGA GGAGCCCCCC
    ATCCCACCCC TGGAGCAGCC GGTGGCCCCT GAGGACAAGG GAGTGGTGAT CAGTGAAGAG
    GCAGCCACAG CTGCCGTCCA GGGGGCTGTG GCCATGGCTG CCCCCATGGC CGGGCTGATG
    GAGAAGGCTG GCAAGGACGG GCCACCCAAG AGCTTCCGGG AGTTGAAAGA GGCCATCAAG
    ATCCTGGAGA GCCTCAAGAA CATGACTGTG GAGCAGCTGC TGACGGGCTC GCCCACCTCT
    CCGACTGTGG AGCCTGAGAA GCCAACTCGG GAGAAGAAGT TTCTGGATGA CATCAAGAAG
    CTACAGGAAA ACCTCAAGAA GACCCTGGAC AATGTGGCCA TTGTAGAGGA GGAGAAGATG
    GAAGCAGTGC CCGACGTAGA GCGCAAGGAG GACAAGCCCG AGGGGCAGTC ACCTGTGAAG
    GCTGAGTGGC CCAGCGAAAC CCCGGTGCTG TGCCAGCAGT GTGGCGGCAA GCCTGGCGTC
    ACCTTCACCA GCGCCAAGGG CGAGGTCTTC TCCGTACTGG AGTTTGCACC CTCAAATCAT
    TCTTTTAAGA AAATTGAGTT CCAGCCTCCA GAAGCCAAGA AGTTCTTCAG CACAGTGCGG
    AAGGAGATGG CGCTGCTGGC TACCTCACTG CCTGAGGGCA TCATGGTCAA GACTTTTGAA
    GATAGAATGG ACCTCTTCTC AGCTCTCATC AAGGGCCCCA CTCGAACCCC CTACGAGGAT
    GGCCTCTACT TGTTTGACAT CCAGCTCCCC AACATCTACC CAGCCGTGCC CCCCCACTTC
    TGCTACCTCT CCCAATGCAG TGGCCGCCTG AACCCCAACC TGTATGACAA TGGGAAGGTG
    TGTGTCAGCC TCCTGGGCAC CTGGATTGGA AAGGGGACAG AGAGGTGGAC AAGCAAGTCC
    AGCCTTCTCC AGGTGCTCAT CTCCATCCAA GGTCTGATCC TGGTAAATGA ACCATACTAC
    AACGAAGCCG GCTTCGACAG TGACCGAGGC CTGCAGGAAG GCTATGAAAA CAGTCGCTGT
    TACAATGAGA TGGCGCTGAT CCGCGTGGTG CAGTCCATGA CCCAGCTGGT GCGGCGGCCC
    CCCGAGGTCT TTGAGCAGGA GATCAGGCAA CACTTTAGCA CTGGTGGCTG GCGGCTGGTG
    AACCGTATCG AGTCCTGGCT GGAAACCCAT GCCCTGCTGG AGAAGGCCCA GGCACTGCCC
    AACGGGGTGC CCAAGGCCAG CAGCTCGCCA GAGCCCCCAG CTGTAGCCGA GCTGTCAGAC
    TCCGGCCAAC AAGAACCTGA GGATGGAGGG CCAGCCCCAG GAGAGGCCTC CCAGGGCTCA
    GACTCAGAGG GCGGTGCCCA GGGCCTGGCC TCAGCTAGCA GGGACCACAC AGACCAGACT
    TCGGAGACCG CACCAGACGC ATCGGTGCCA CCCAGTGTGA AACCAAAGAA GCGGAGAAAG
    AGCTACCGGA GCTTCTTACC TGAGAAGAGT GGCTACCCTG ACATCGGCTT CCCCCTCTTC
    CCACTTTCCA AGGGTTTCAT CAAGAGCATC CGGGGTGTCC TGACGCAGTT CCGGGCTGCC
    CTGCTAGAGG CAGGCATGCC GGAGTGCACA GAGGACAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71127
    Clone ID Related Accession (Same CDS sequence) XM_024450865.1
    Accession Version XM_024450865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA
    CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC
    AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC
    CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC
    ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC
    TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT
    GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA
    TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC
    CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG
    GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC
    GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG
    AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA
    CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT
    GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC
    AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG
    AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC
    CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG
    AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG
    ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG
    CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT
    GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC
    CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG
    CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG
    GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG
    GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC
    TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC
    GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG
    AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC
    ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT
    ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCAGCCAT CGGTGGGCCA GGTGGCCCGT
    GTGGACGTCA GCAGCAAGGT GGAGGTGGTG TGGGCTGACA ACTCAAAGAC CATCATCCTG
    CCCCAGCACT TGTACAACAT AGAGTCTGAG ATTGAGGAGT CAGACTACGA TTCGGTAGAA
    GGCAGCACCA GCGGGGCATC CTCGGATGAA TGGGAAGATG ATAGTGACAG CTGGGAGACG
    GACAATGGGC TGGTGGAGGA CGAGCACCCC AAGATAGAGG AGCCCCCCAT CCCACCCCTG
    GAGCAGCCGG TGGCCCCTGA GGACAAGGGA GTGGTGATCA GTGAAGAGGC AGCCACAGCT
    GCCGTCCAGG GGGCTGTGGC CATGGCTGCC CCCATGGCCG GGCTGATGGA GAAGGCTGGC
    AAGGACGGGC CACCCAAGAG CTTCCGGGAG TTGAAAGAGG CCATCAAGAT CCTGGAGAGC
    CTCAAGAACA TGACTGTGGA GCAGCTGCTG ACGGGCTCGC CCACCTCTCC GACTGTGGAG
    CCTGAGAAGC CAACTCGGGA GAAGAAGTTT CTGGATGACA TCAAGAAGCT ACAGGAAAAC
    CTCAAGAAGA CCCTGGACAA TGTGGCCATT GTAGAGGAGG AGAAGATGGA AGCAGTGCCC
    GACGTAGAGC GCAAGGAGGA CAAGCCCGAG GGGCAGTCAC CTGTGAAGGC TGAGTGGCCC
    AGCGAAACCC CGGTGCTGTG CCAGCAGTGT GGCGGCAAGC CTGGCGTCAC CTTCACCAGC
    GCCAAGGGCG AGGTCTTCTC CGTACTGGAG TTTGCACCCT CAAATCATTC TTTTAAGAAA
    ATTGAGTTCC AGCCTCCAGA AGCCAAGAAG TTCTTCAGCA CAGTGCGGAA GGAGATGGCG
    CTGCTGGCTA CCTCACTGCC TGAGGGCATC ATGGTCAAGA CTTTTGAAGA TAGAATGGAC
    CTCTTCTCAG CTCTCATCAA GGGCCCCACT CGAACCCCCT ACGAGGATGG CCTCTACTTG
    TTTGACATCC AGCTCCCCAA CATCTACCCA GCCGTGCCCC CCCACTTCTG CTACCTCTCC
    CAATGCAGTG GCCGCCTGAA CCCCAACCTG TATGACAATG GGAAGGTGTG TGTCAGCCTC
    CTGGGCACCT GGATTGGAAA GGGGACAGAG AGGTGGACAA GCAAGTCCAG CCTTCTCCAG
    GTGCTCATCT CCATCCAAGG TCTGATCCTG GTAAATGAAC CATACTACAA CGAAGCCGGC
    TTCGACAGTG ACCGAGGCCT GCAGGAAGGC TATGAAAACA GTCGCTGTTA CAATGAGATG
    GCGCTGATCC GCGTGGTGCA GTCCATGACC CAGCTGGTGC GGCGGCCCCC CGAGGTCTTT
    GAGCAGGAGA TCAGGCAACA CTTTAGCACT GGTGGCTGGC GGCTGGTGAA CCGTATCGAG
    TCCTGGCTGG AAACCCATGC CCTGCTGGAG AAGGCCCAGG CACTGCCCAA CGGGGTGCCC
    AAGGCCAGCA GCTCGCCAGA GCCCCCAGCT GTAGCCGAGC TGTCAGACTC CGGCCAACAA
    GAACCTGAGG ATGGAGGGCC AGCCCCAGGA GAGGCCTCCC AGGGCTCAGA CTCAGAGGGC
    GGTGCCCAGG GCCTGGCCTC AGCTAGCAGG GACCACACAG ACCAGACTTC GGAGACCGCA
    CCAGACGCAT CGGTGCCACC CAGTGTGAAA CCAAAGAAGC GGAGAAAGAG CTACCGGAGC
    TTCTTACCTG AGAAGAGTGG CTACCCTGAC ATCGGCTTCC CCCTCTTCCC ACTTTCCAAG
    GGTTTCATCA AGAGCATCCG GGGTGTCCTG ACGCAGTTCC GGGCTGCCCT GCTAGAGGCA
    GGCATGCCGG AGTGCACAGA GGACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306633.1
    CDS94..4083
    Translation

    Target ORF information:

    RefSeq Version XM_024450865.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA
    CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC
    AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC
    CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC
    ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC
    TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT
    GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA
    TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC
    CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG
    GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC
    GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG
    AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA
    CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT
    GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC
    AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG
    AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC
    CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG
    AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG
    ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG
    CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT
    GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC
    CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG
    CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG
    GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG
    GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC
    TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC
    GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG
    AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC
    ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT
    ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCAGCCAT CGGTGGGCCA GGTGGCCCGT
    GTGGACGTCA GCAGCAAGGT GGAGGTGGTG TGGGCTGACA ACTCAAAGAC CATCATCCTG
    CCCCAGCACT TGTACAACAT AGAGTCTGAG ATTGAGGAGT CAGACTACGA TTCGGTAGAA
    GGCAGCACCA GCGGGGCATC CTCGGATGAA TGGGAAGATG ATAGTGACAG CTGGGAGACG
    GACAATGGGC TGGTGGAGGA CGAGCACCCC AAGATAGAGG AGCCCCCCAT CCCACCCCTG
    GAGCAGCCGG TGGCCCCTGA GGACAAGGGA GTGGTGATCA GTGAAGAGGC AGCCACAGCT
    GCCGTCCAGG GGGCTGTGGC CATGGCTGCC CCCATGGCCG GGCTGATGGA GAAGGCTGGC
    AAGGACGGGC CACCCAAGAG CTTCCGGGAG TTGAAAGAGG CCATCAAGAT CCTGGAGAGC
    CTCAAGAACA TGACTGTGGA GCAGCTGCTG ACGGGCTCGC CCACCTCTCC GACTGTGGAG
    CCTGAGAAGC CAACTCGGGA GAAGAAGTTT CTGGATGACA TCAAGAAGCT ACAGGAAAAC
    CTCAAGAAGA CCCTGGACAA TGTGGCCATT GTAGAGGAGG AGAAGATGGA AGCAGTGCCC
    GACGTAGAGC GCAAGGAGGA CAAGCCCGAG GGGCAGTCAC CTGTGAAGGC TGAGTGGCCC
    AGCGAAACCC CGGTGCTGTG CCAGCAGTGT GGCGGCAAGC CTGGCGTCAC CTTCACCAGC
    GCCAAGGGCG AGGTCTTCTC CGTACTGGAG TTTGCACCCT CAAATCATTC TTTTAAGAAA
    ATTGAGTTCC AGCCTCCAGA AGCCAAGAAG TTCTTCAGCA CAGTGCGGAA GGAGATGGCG
    CTGCTGGCTA CCTCACTGCC TGAGGGCATC ATGGTCAAGA CTTTTGAAGA TAGAATGGAC
    CTCTTCTCAG CTCTCATCAA GGGCCCCACT CGAACCCCCT ACGAGGATGG CCTCTACTTG
    TTTGACATCC AGCTCCCCAA CATCTACCCA GCCGTGCCCC CCCACTTCTG CTACCTCTCC
    CAATGCAGTG GCCGCCTGAA CCCCAACCTG TATGACAATG GGAAGGTGTG TGTCAGCCTC
    CTGGGCACCT GGATTGGAAA GGGGACAGAG AGGTGGACAA GCAAGTCCAG CCTTCTCCAG
    GTGCTCATCT CCATCCAAGG TCTGATCCTG GTAAATGAAC CATACTACAA CGAAGCCGGC
    TTCGACAGTG ACCGAGGCCT GCAGGAAGGC TATGAAAACA GTCGCTGTTA CAATGAGATG
    GCGCTGATCC GCGTGGTGCA GTCCATGACC CAGCTGGTGC GGCGGCCCCC CGAGGTCTTT
    GAGCAGGAGA TCAGGCAACA CTTTAGCACT GGTGGCTGGC GGCTGGTGAA CCGTATCGAG
    TCCTGGCTGG AAACCCATGC CCTGCTGGAG AAGGCCCAGG CACTGCCCAA CGGGGTGCCC
    AAGGCCAGCA GCTCGCCAGA GCCCCCAGCT GTAGCCGAGC TGTCAGACTC CGGCCAACAA
    GAACCTGAGG ATGGAGGGCC AGCCCCAGGA GAGGCCTCCC AGGGCTCAGA CTCAGAGGGC
    GGTGCCCAGG GCCTGGCCTC AGCTAGCAGG GACCACACAG ACCAGACTTC GGAGACCGCA
    CCAGACGCAT CGGTGCCACC CAGTGTGAAA CCAAAGAAGC GGAGAAAGAG CTACCGGAGC
    TTCTTACCTG AGAAGAGTGG CTACCCTGAC ATCGGCTTCC CCCTCTTCCC ACTTTCCAAG
    GGTTTCATCA AGAGCATCCG GGGTGTCCTG ACGCAGTTCC GGGCTGCCCT GCTAGAGGCA
    GGCATGCCGG AGTGCACAGA GGACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71128
    Clone ID Related Accession (Same CDS sequence) XM_024450866.1
    Accession Version XM_024450866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA
    CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC
    AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC
    CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC
    ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC
    TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT
    GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA
    TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC
    CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG
    GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC
    GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG
    AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA
    CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT
    GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC
    AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG
    AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC
    CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG
    AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG
    ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG
    CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT
    GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC
    CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG
    CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG
    GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG
    GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC
    TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC
    GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG
    AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC
    ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT
    ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCCATCGG TGGGCCAGGT GGCCCGTGTG
    GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC
    CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC
    AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC
    AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG
    CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC
    GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG
    GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC
    AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT
    GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC
    AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC
    GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC
    GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC
    AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT
    GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG
    CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC
    TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT
    GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA
    TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG
    GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG
    CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC
    GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG
    CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG
    CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC
    TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG
    GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA
    CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT
    GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA
    GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC
    TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT
    TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC
    ATGCCGGAGT GCACAGAGGA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306634.1
    CDS94..4080
    Translation

    Target ORF information:

    RefSeq Version XM_024450866.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA
    CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC
    AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC
    CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC
    ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC
    TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT
    GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA
    TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC
    CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG
    GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC
    GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG
    AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA
    CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT
    GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC
    AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG
    AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC
    CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG
    AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG
    ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG
    CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT
    GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC
    CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG
    CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG
    GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG
    GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC
    TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC
    GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG
    AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC
    ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT
    ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCCATCGG TGGGCCAGGT GGCCCGTGTG
    GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC
    CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC
    AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC
    AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG
    CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC
    GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG
    GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC
    AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT
    GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC
    AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC
    GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC
    GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC
    AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT
    GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG
    CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC
    TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT
    GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA
    TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG
    GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG
    CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC
    GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG
    CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG
    CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC
    TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG
    GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA
    CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT
    GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA
    GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC
    TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT
    TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC
    ATGCCGGAGT GCACAGAGGA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71129
    Clone ID Related Accession (Same CDS sequence) XM_024450867.1
    Accession Version XM_024450867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA
    CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC
    AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC
    CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC
    ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC
    TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT
    GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA
    TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC
    CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG
    GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC
    GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG
    AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA
    CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT
    GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT GAAGCGCCTG
    TTGAAGAAGC AGGTTGTGCG GATCATGTCA TGCTCCCCAG ACACCCAGTG TTCCCGGGAC
    CATTCCATGG AAGACCCAGA CAAGAAGGGG GAATCCAAAA CCAAGAGCGA AGCGGAGTCT
    GCCAGCCCTG AGGAGACGCC CGATGGCTCT GCCAGTCCAG TGGAGATGCA GGACGAGGGT
    GCAGAGGAGC CCCACGAGGC AGGAGAGCAG CTGCCCCCAT TCCTGCTAAA AGAAGGCAGA
    GATGACAGGC TGCACTCGGC AGAGCAGGAC GCAGATGATG AGGCTGCTGA TGACACGGAC
    GACACCAGTT CGGTGACCTC CTCTGCCAGC TCCACCACTT CCTCCCAGAG CGGCAGCGGC
    ACGAGTCGCA AAAAGAGCAT CCCCTTGTCC ATCAAGAACT TAAAGCGCAA ACACAAGAGG
    AAGAAGAATA AAATCACTCG AGACTTCAAG CCAGGGGACA GGGTGGCAGT GGAGGTGGTG
    ACCACGATGA CCTCAGCCGA CGTGATGTGG CAGGATGGCT CCGTGGAATG CAACATCCGC
    TCCAACGACC TCTTCCCTGT GCACCACCTG GACAACAACG AGTTCTGCCC TGGAGACTTC
    GTGGTAGATA AGCGAGTCCA GAGCTGTCCA GACCCTGCTG TCTACGGTGT GGTACAGTCT
    GGGGACCACA TCGGCCGTAC CTGCATGGTG AAGTGGTTCA AGCTGAGGCC GAGTGGGGAC
    GACGTGGAGC TGATTGGAGA AGAGGAAGAT GTGAGTGTTT ACGACATTGC TGACCACCCT
    GACTTTAGGT TCCGTACAAC TGACATCGTC ATCCGCATCG GCAATACTGA GGATGGGGCT
    CCTCACAAGG AGGATGAGCA GCCATCGGTG GGCCAGGTGG CCCGTGTGGA CGTCAGCAGC
    AAGGTGGAGG TGGTGTGGGC TGACAACTCA AAGACCATCA TCCTGCCCCA GCACTTGTAC
    AACATAGAGT CTGAGATTGA GGAGTCAGAC TACGATTCGG TAGAAGGCAG CACCAGCGGG
    GCATCCTCGG ATGAATGGGA AGATGATAGT GACAGCTGGG AGACGGACAA TGGGCTGGTG
    GAGGACGAGC ACCCCAAGAT AGAGGAGCCC CCCATCCCAC CCCTGGAGCA GCCGGTGGCC
    CCTGAGGACA AGGGAGTGGT GATCAGTGAA GAGGCAGCCA CAGCTGCCGT CCAGGGGGCT
    GTGGCCATGG CTGCCCCCAT GGCCGGGCTG ATGGAGAAGG CTGGCAAGGA CGGGCCACCC
    AAGAGCTTCC GGGAGTTGAA AGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT
    GTGGAGCAGC TGCTGACGGG CTCGCCCACC TCTCCGACTG TGGAGCCTGA GAAGCCAACT
    CGGGAGAAGA AGTTTCTGGA TGACATCAAG AAGCTACAGG AAAACCTCAA GAAGACCCTG
    GACAATGTGG CCATTGTAGA GGAGGAGAAG ATGGAAGCAG TGCCCGACGT AGAGCGCAAG
    GAGGACAAGC CCGAGGGGCA GTCACCTGTG AAGGCTGAGT GGCCCAGCGA AACCCCGGTG
    CTGTGCCAGC AGTGTGGCGG CAAGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC
    TTCTCCGTAC TGGAGTTTGC ACCCTCAAAT CATTCTTTTA AGAAAATTGA GTTCCAGCCT
    CCAGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCGCTGCT GGCTACCTCA
    CTGCCTGAGG GCATCATGGT CAAGACTTTT GAAGATAGAA TGGACCTCTT CTCAGCTCTC
    ATCAAGGGCC CCACTCGAAC CCCCTACGAG GATGGCCTCT ACTTGTTTGA CATCCAGCTC
    CCCAACATCT ACCCAGCCGT GCCCCCCCAC TTCTGCTACC TCTCCCAATG CAGTGGCCGC
    CTGAACCCCA ACCTGTATGA CAATGGGAAG GTGTGTGTCA GCCTCCTGGG CACCTGGATT
    GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCCAGCCTTC TCCAGGTGCT CATCTCCATC
    CAAGGTCTGA TCCTGGTAAA TGAACCATAC TACAACGAAG CCGGCTTCGA CAGTGACCGA
    GGCCTGCAGG AAGGCTATGA AAACAGTCGC TGTTACAATG AGATGGCGCT GATCCGCGTG
    GTGCAGTCCA TGACCCAGCT GGTGCGGCGG CCCCCCGAGG TCTTTGAGCA GGAGATCAGG
    CAACACTTTA GCACTGGTGG CTGGCGGCTG GTGAACCGTA TCGAGTCCTG GCTGGAAACC
    CATGCCCTGC TGGAGAAGGC CCAGGCACTG CCCAACGGGG TGCCCAAGGC CAGCAGCTCG
    CCAGAGCCCC CAGCTGTAGC CGAGCTGTCA GACTCCGGCC AACAAGAACC TGAGGATGGA
    GGGCCAGCCC CAGGAGAGGC CTCCCAGGGC TCAGACTCAG AGGGCGGTGC CCAGGGCCTG
    GCCTCAGCTA GCAGGGACCA CACAGACCAG ACTTCGGAGA CCGCACCAGA CGCATCGGTG
    CCACCCAGTG TGAAACCAAA GAAGCGGAGA AAGAGCTACC GGAGCTTCTT ACCTGAGAAG
    AGTGGCTACC CTGACATCGG CTTCCCCCTC TTCCCACTTT CCAAGGGTTT CATCAAGAGC
    ATCCGGGGTG TCCTGACGCA GTTCCGGGCT GCCCTGCTAG AGGCAGGCAT GCCGGAGTGC
    ACAGAGGACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306635.1
    CDS94..4008
    Translation

    Target ORF information:

    RefSeq Version XM_024450867.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGGCA
    CAACAGCAGC AAGCAGCTCC TCTAAATGCA GTGAGCCAGG ACTCAAATAC CAGCGGAAAC
    AGCACCATCA TGGAGACTGG GAGGAGTTTC TGCCCAATTC TGCAGCTGAA ACTAGAGGAC
    CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC
    ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC
    TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT
    GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA
    TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC
    CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG
    GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC
    GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG
    AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA
    CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT
    GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT GAAGCGCCTG
    TTGAAGAAGC AGGTTGTGCG GATCATGTCA TGCTCCCCAG ACACCCAGTG TTCCCGGGAC
    CATTCCATGG AAGACCCAGA CAAGAAGGGG GAATCCAAAA CCAAGAGCGA AGCGGAGTCT
    GCCAGCCCTG AGGAGACGCC CGATGGCTCT GCCAGTCCAG TGGAGATGCA GGACGAGGGT
    GCAGAGGAGC CCCACGAGGC AGGAGAGCAG CTGCCCCCAT TCCTGCTAAA AGAAGGCAGA
    GATGACAGGC TGCACTCGGC AGAGCAGGAC GCAGATGATG AGGCTGCTGA TGACACGGAC
    GACACCAGTT CGGTGACCTC CTCTGCCAGC TCCACCACTT CCTCCCAGAG CGGCAGCGGC
    ACGAGTCGCA AAAAGAGCAT CCCCTTGTCC ATCAAGAACT TAAAGCGCAA ACACAAGAGG
    AAGAAGAATA AAATCACTCG AGACTTCAAG CCAGGGGACA GGGTGGCAGT GGAGGTGGTG
    ACCACGATGA CCTCAGCCGA CGTGATGTGG CAGGATGGCT CCGTGGAATG CAACATCCGC
    TCCAACGACC TCTTCCCTGT GCACCACCTG GACAACAACG AGTTCTGCCC TGGAGACTTC
    GTGGTAGATA AGCGAGTCCA GAGCTGTCCA GACCCTGCTG TCTACGGTGT GGTACAGTCT
    GGGGACCACA TCGGCCGTAC CTGCATGGTG AAGTGGTTCA AGCTGAGGCC GAGTGGGGAC
    GACGTGGAGC TGATTGGAGA AGAGGAAGAT GTGAGTGTTT ACGACATTGC TGACCACCCT
    GACTTTAGGT TCCGTACAAC TGACATCGTC ATCCGCATCG GCAATACTGA GGATGGGGCT
    CCTCACAAGG AGGATGAGCA GCCATCGGTG GGCCAGGTGG CCCGTGTGGA CGTCAGCAGC
    AAGGTGGAGG TGGTGTGGGC TGACAACTCA AAGACCATCA TCCTGCCCCA GCACTTGTAC
    AACATAGAGT CTGAGATTGA GGAGTCAGAC TACGATTCGG TAGAAGGCAG CACCAGCGGG
    GCATCCTCGG ATGAATGGGA AGATGATAGT GACAGCTGGG AGACGGACAA TGGGCTGGTG
    GAGGACGAGC ACCCCAAGAT AGAGGAGCCC CCCATCCCAC CCCTGGAGCA GCCGGTGGCC
    CCTGAGGACA AGGGAGTGGT GATCAGTGAA GAGGCAGCCA CAGCTGCCGT CCAGGGGGCT
    GTGGCCATGG CTGCCCCCAT GGCCGGGCTG ATGGAGAAGG CTGGCAAGGA CGGGCCACCC
    AAGAGCTTCC GGGAGTTGAA AGAGGCCATC AAGATCCTGG AGAGCCTCAA GAACATGACT
    GTGGAGCAGC TGCTGACGGG CTCGCCCACC TCTCCGACTG TGGAGCCTGA GAAGCCAACT
    CGGGAGAAGA AGTTTCTGGA TGACATCAAG AAGCTACAGG AAAACCTCAA GAAGACCCTG
    GACAATGTGG CCATTGTAGA GGAGGAGAAG ATGGAAGCAG TGCCCGACGT AGAGCGCAAG
    GAGGACAAGC CCGAGGGGCA GTCACCTGTG AAGGCTGAGT GGCCCAGCGA AACCCCGGTG
    CTGTGCCAGC AGTGTGGCGG CAAGCCTGGC GTCACCTTCA CCAGCGCCAA GGGCGAGGTC
    TTCTCCGTAC TGGAGTTTGC ACCCTCAAAT CATTCTTTTA AGAAAATTGA GTTCCAGCCT
    CCAGAAGCCA AGAAGTTCTT CAGCACAGTG CGGAAGGAGA TGGCGCTGCT GGCTACCTCA
    CTGCCTGAGG GCATCATGGT CAAGACTTTT GAAGATAGAA TGGACCTCTT CTCAGCTCTC
    ATCAAGGGCC CCACTCGAAC CCCCTACGAG GATGGCCTCT ACTTGTTTGA CATCCAGCTC
    CCCAACATCT ACCCAGCCGT GCCCCCCCAC TTCTGCTACC TCTCCCAATG CAGTGGCCGC
    CTGAACCCCA ACCTGTATGA CAATGGGAAG GTGTGTGTCA GCCTCCTGGG CACCTGGATT
    GGAAAGGGGA CAGAGAGGTG GACAAGCAAG TCCAGCCTTC TCCAGGTGCT CATCTCCATC
    CAAGGTCTGA TCCTGGTAAA TGAACCATAC TACAACGAAG CCGGCTTCGA CAGTGACCGA
    GGCCTGCAGG AAGGCTATGA AAACAGTCGC TGTTACAATG AGATGGCGCT GATCCGCGTG
    GTGCAGTCCA TGACCCAGCT GGTGCGGCGG CCCCCCGAGG TCTTTGAGCA GGAGATCAGG
    CAACACTTTA GCACTGGTGG CTGGCGGCTG GTGAACCGTA TCGAGTCCTG GCTGGAAACC
    CATGCCCTGC TGGAGAAGGC CCAGGCACTG CCCAACGGGG TGCCCAAGGC CAGCAGCTCG
    CCAGAGCCCC CAGCTGTAGC CGAGCTGTCA GACTCCGGCC AACAAGAACC TGAGGATGGA
    GGGCCAGCCC CAGGAGAGGC CTCCCAGGGC TCAGACTCAG AGGGCGGTGC CCAGGGCCTG
    GCCTCAGCTA GCAGGGACCA CACAGACCAG ACTTCGGAGA CCGCACCAGA CGCATCGGTG
    CCACCCAGTG TGAAACCAAA GAAGCGGAGA AAGAGCTACC GGAGCTTCTT ACCTGAGAAG
    AGTGGCTACC CTGACATCGG CTTCCCCCTC TTCCCACTTT CCAAGGGTTT CATCAAGAGC
    ATCCGGGGTG TCCTGACGCA GTTCCGGGCT GCCCTGCTAG AGGCAGGCAT GCCGGAGTGC
    ACAGAGGACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87027
    Clone ID Related Accession (Same CDS sequence) XM_017024934.1
    Accession Version XM_017024934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG
    TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC
    GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG
    CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA
    AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT
    GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG
    AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC
    AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA
    GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA
    TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC
    CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT
    GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG
    CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT
    GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT
    GAGGATGGGG CTCCTCACAA GGAGGATGAG CAGCCATCGG TGGGCCAGGT GGCCCGTGTG
    GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC
    CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC
    AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC
    AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG
    CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC
    GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG
    GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC
    AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT
    GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC
    AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC
    GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC
    GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC
    AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT
    GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG
    CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC
    TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT
    GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA
    TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG
    GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG
    CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC
    GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG
    CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG
    CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC
    TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG
    GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA
    CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT
    GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA
    GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC
    TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT
    TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC
    ATGCCGGAGT GCACAGAGGA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880423.1
    CDS50..3856
    Translation

    Target ORF information:

    RefSeq Version XM_017024934.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG
    TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC
    GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG
    CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA
    AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT
    GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG
    AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC
    AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA
    GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA
    TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC
    CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT
    GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG
    CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT
    GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT
    GAGGATGGGG CTCCTCACAA GGAGGATGAG CAGCCATCGG TGGGCCAGGT GGCCCGTGTG
    GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC
    CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC
    AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC
    AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG
    CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC
    GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG
    GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC
    AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT
    GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC
    AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC
    GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC
    GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC
    AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT
    GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG
    CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC
    TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT
    GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA
    TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG
    GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG
    CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC
    GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG
    CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG
    CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC
    TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG
    GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA
    CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT
    GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA
    GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC
    TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT
    TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC
    ATGCCGGAGT GCACAGAGGA CAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87028
    Clone ID Related Accession (Same CDS sequence) XM_017024935.1
    Accession Version XM_017024935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG
    TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC
    GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG
    CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA
    AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT
    GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG
    AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC
    AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA
    GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA
    TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC
    CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT
    GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG
    CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT
    GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT
    GAGGATGGGG CTCCTCACAA GGAGGATGAG CCATCGGTGG GCCAGGTGGC CCGTGTGGAC
    GTCAGCAGCA AGGTGGAGGT GGTGTGGGCT GACAACTCAA AGACCATCAT CCTGCCCCAG
    CACTTGTACA ACATAGAGTC TGAGATTGAG GAGTCAGACT ACGATTCGGT AGAAGGCAGC
    ACCAGCGGGG CATCCTCGGA TGAATGGGAA GATGATAGTG ACAGCTGGGA GACGGACAAT
    GGGCTGGTGG AGGACGAGCA CCCCAAGATA GAGGAGCCCC CCATCCCACC CCTGGAGCAG
    CCGGTGGCCC CTGAGGACAA GGGAGTGGTG ATCAGTGAAG AGGCAGCCAC AGCTGCCGTC
    CAGGGGGCTG TGGCCATGGC TGCCCCCATG GCCGGGCTGA TGGAGAAGGC TGGCAAGGAC
    GGGCCACCCA AGAGCTTCCG GGAGTTGAAA GAGGCCATCA AGATCCTGGA GAGCCTCAAG
    AACATGACTG TGGAGCAGCT GCTGACGGGC TCGCCCACCT CTCCGACTGT GGAGCCTGAG
    AAGCCAACTC GGGAGAAGAA GTTTCTGGAT GACATCAAGA AGCTACAGGA AAACCTCAAG
    AAGACCCTGG ACAATGTGGC CATTGTAGAG GAGGAGAAGA TGGAAGCAGT GCCCGACGTA
    GAGCGCAAGG AGGACAAGCC CGAGGGGCAG TCACCTGTGA AGGCTGAGTG GCCCAGCGAA
    ACCCCGGTGC TGTGCCAGCA GTGTGGCGGC AAGCCTGGCG TCACCTTCAC CAGCGCCAAG
    GGCGAGGTCT TCTCCGTACT GGAGTTTGCA CCCTCAAATC ATTCTTTTAA GAAAATTGAG
    TTCCAGCCTC CAGAAGCCAA GAAGTTCTTC AGCACAGTGC GGAAGGAGAT GGCGCTGCTG
    GCTACCTCAC TGCCTGAGGG CATCATGGTC AAGACTTTTG AAGATAGAAT GGACCTCTTC
    TCAGCTCTCA TCAAGGGCCC CACTCGAACC CCCTACGAGG ATGGCCTCTA CTTGTTTGAC
    ATCCAGCTCC CCAACATCTA CCCAGCCGTG CCCCCCCACT TCTGCTACCT CTCCCAATGC
    AGTGGCCGCC TGAACCCCAA CCTGTATGAC AATGGGAAGG TGTGTGTCAG CCTCCTGGGC
    ACCTGGATTG GAAAGGGGAC AGAGAGGTGG ACAAGCAAGT CCAGCCTTCT CCAGGTGCTC
    ATCTCCATCC AAGGTCTGAT CCTGGTAAAT GAACCATACT ACAACGAAGC CGGCTTCGAC
    AGTGACCGAG GCCTGCAGGA AGGCTATGAA AACAGTCGCT GTTACAATGA GATGGCGCTG
    ATCCGCGTGG TGCAGTCCAT GACCCAGCTG GTGCGGCGGC CCCCCGAGGT CTTTGAGCAG
    GAGATCAGGC AACACTTTAG CACTGGTGGC TGGCGGCTGG TGAACCGTAT CGAGTCCTGG
    CTGGAAACCC ATGCCCTGCT GGAGAAGGCC CAGGCACTGC CCAACGGGGT GCCCAAGGCC
    AGCAGCTCGC CAGAGCCCCC AGCTGTAGCC GAGCTGTCAG ACTCCGGCCA ACAAGAACCT
    GAGGATGGAG GGCCAGCCCC AGGAGAGGCC TCCCAGGGCT CAGACTCAGA GGGCGGTGCC
    CAGGGCCTGG CCTCAGCTAG CAGGGACCAC ACAGACCAGA CTTCGGAGAC CGCACCAGAC
    GCATCGGTGC CACCCAGTGT GAAACCAAAG AAGCGGAGAA AGAGCTACCG GAGCTTCTTA
    CCTGAGAAGA GTGGCTACCC TGACATCGGC TTCCCCCTCT TCCCACTTTC CAAGGGTTTC
    ATCAAGAGCA TCCGGGGTGT CCTGACGCAG TTCCGGGCTG CCCTGCTAGA GGCAGGCATG
    CCGGAGTGCA CAGAGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880424.1
    CDS50..3853
    Translation

    Target ORF information:

    RefSeq Version XM_017024935.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCCCC CACGCCCGCA GCTCCCGCTC CAGCCCAGGC CCCGGCTCCA 
    GCCCCGGAGG CAGTCCCGGC CCCAGCCGCA GCCCCCGTCC CGGCGCCGGC GCCCGCCTCG
    GACTCGGCCT CCGGGCCGTC CTCGGACTCC GGCCCAGAAG CCGGCTCGCA GCGCCTGCTG
    TTTTCTCACG ACCTGGTGTC GGGCCGTTAC CGTGGCTCCG TGCACTTCGG GCTGGTGCGC
    CTCATCCACG GCGAGGACTC GGACTCGGAG GGCGAGGAGG AGGGCCGCGG GAGCTCGGGG
    TGCTCCGAGG CCGGGGGCGC GGGCCACGAG GAGGGCCGGG CCAGCCCCCT GCGCCGCGGC
    TACGTGCGCG TCCAGTGGTA CCCGGAGGGC GTCAAGCAGC ATGTGAAGGA GACCAAGCTG
    AAACTAGAGG ACCGTTCTGT GGTGCCCCGA GATGTGGTCC GGCACATGCG ATCCACCGAC
    AGTCAGTGTG GCACGGTGAT CGACGTCAAC ATCGACTGTG CCGTCAAGCT CATCGGCACC
    AACTGCATCA TCTATCCCGT CAACAGCAAG GACCTGCAGC ACATCTGGCC CTTCATGTAT
    GGGGACTACA TTGCCTATGA CTGCTGGCTG GGGAAGGTCT ACGACTTGAA GAACCAGATC
    ATCCTGAAGC TATCCAACGG CGCCAGGTGC TCCATGAACA CGGAAGATGG CGCCAAGCTC
    TACGACGTCT GCCCGCACGT CAGCGACTCG GGTCTCTTCT TCGATGATTC CTATGGCTTC
    TACCCAGGCC AGGTGCTCAT TGGCCCTGCC AAGATCTTCT CCAGCGTCCA GTGGCTGTCA
    GGTGTCAAGC CCGTGCTCAG CACCAAGAGC AAGTTCCGAG TGGTGGTGGA AGAGGTGCAG
    GTTGTAGAGT TGAAAGTTAC ATGGATTACC AAGAGTTTCT GTCCAGGGGG CACGGACAGC
    GTCAGCCCCC CACCCTCTGT CATCACCCAG GAAAACCTAG GCAGGGTGAA GCGTCTCGGA
    TGCTTTGACC ATGCTCAGCG GCAGCTTGGG GAGCGCTGTC TGTATGTCTT CCCAGCCAAG
    GTGAAGCGCC TGTTGAAGAA GCAGGTTGTG CGGATCATGT CATGCTCCCC AGACACCCAG
    TGTTCCCGGG ACCATTCCAT GGAAGACCCA GACAAGAAGG GGGAATCCAA AACCAAGAGC
    GAAGCGGAGT CTGCCAGCCC TGAGGAGACG CCCGATGGCT CTGCCAGTCC AGTGGAGATG
    CAGGACGAGG GTGCAGAGGA GCCCCACGAG GCAGGAGAGC AGCTGCCCCC ATTCCTGCTA
    AAAGAAGGCA GAGATGACAG GCTGCACTCG GCAGAGCAGG ACGCAGATGA TGAGGCTGCT
    GATGACACGG ACGACACCAG TTCGGTGACC TCCTCTGCCA GCTCCACCAC TTCCTCCCAG
    AGCGGCAGCG GCACGAGTCG CAAAAAGAGC ATCCCCTTGT CCATCAAGAA CTTAAAGCGC
    AAACACAAGA GGAAGAAGAA TAAAATCACT CGAGACTTCA AGCCAGGGGA CAGGGTGGCA
    GTGGAGGTGG TGACCACGAT GACCTCAGCC GACGTGATGT GGCAGGATGG CTCCGTGGAA
    TGCAACATCC GCTCCAACGA CCTCTTCCCT GTGCACCACC TGGACAACAA CGAGTTCTGC
    CCTGGAGACT TCGTGGTAGA TAAGCGAGTC CAGAGCTGTC CAGACCCTGC TGTCTACGGT
    GTGGTACAGT CTGGGGACCA CATCGGCCGT ACCTGCATGG TGAAGTGGTT CAAGCTGAGG
    CCGAGTGGGG ACGACGTGGA GCTGATTGGA GAAGAGGAAG ATGTGAGTGT TTACGACATT
    GCTGACCACC CTGACTTTAG GTTCCGTACA ACTGACATCG TCATCCGCAT CGGCAATACT
    GAGGATGGGG CTCCTCACAA GGAGGATGAG CCATCGGTGG GCCAGGTGGC CCGTGTGGAC
    GTCAGCAGCA AGGTGGAGGT GGTGTGGGCT GACAACTCAA AGACCATCAT CCTGCCCCAG
    CACTTGTACA ACATAGAGTC TGAGATTGAG GAGTCAGACT ACGATTCGGT AGAAGGCAGC
    ACCAGCGGGG CATCCTCGGA TGAATGGGAA GATGATAGTG ACAGCTGGGA GACGGACAAT
    GGGCTGGTGG AGGACGAGCA CCCCAAGATA GAGGAGCCCC CCATCCCACC CCTGGAGCAG
    CCGGTGGCCC CTGAGGACAA GGGAGTGGTG ATCAGTGAAG AGGCAGCCAC AGCTGCCGTC
    CAGGGGGCTG TGGCCATGGC TGCCCCCATG GCCGGGCTGA TGGAGAAGGC TGGCAAGGAC
    GGGCCACCCA AGAGCTTCCG GGAGTTGAAA GAGGCCATCA AGATCCTGGA GAGCCTCAAG
    AACATGACTG TGGAGCAGCT GCTGACGGGC TCGCCCACCT CTCCGACTGT GGAGCCTGAG
    AAGCCAACTC GGGAGAAGAA GTTTCTGGAT GACATCAAGA AGCTACAGGA AAACCTCAAG
    AAGACCCTGG ACAATGTGGC CATTGTAGAG GAGGAGAAGA TGGAAGCAGT GCCCGACGTA
    GAGCGCAAGG AGGACAAGCC CGAGGGGCAG TCACCTGTGA AGGCTGAGTG GCCCAGCGAA
    ACCCCGGTGC TGTGCCAGCA GTGTGGCGGC AAGCCTGGCG TCACCTTCAC CAGCGCCAAG
    GGCGAGGTCT TCTCCGTACT GGAGTTTGCA CCCTCAAATC ATTCTTTTAA GAAAATTGAG
    TTCCAGCCTC CAGAAGCCAA GAAGTTCTTC AGCACAGTGC GGAAGGAGAT GGCGCTGCTG
    GCTACCTCAC TGCCTGAGGG CATCATGGTC AAGACTTTTG AAGATAGAAT GGACCTCTTC
    TCAGCTCTCA TCAAGGGCCC CACTCGAACC CCCTACGAGG ATGGCCTCTA CTTGTTTGAC
    ATCCAGCTCC CCAACATCTA CCCAGCCGTG CCCCCCCACT TCTGCTACCT CTCCCAATGC
    AGTGGCCGCC TGAACCCCAA CCTGTATGAC AATGGGAAGG TGTGTGTCAG CCTCCTGGGC
    ACCTGGATTG GAAAGGGGAC AGAGAGGTGG ACAAGCAAGT CCAGCCTTCT CCAGGTGCTC
    ATCTCCATCC AAGGTCTGAT CCTGGTAAAT GAACCATACT ACAACGAAGC CGGCTTCGAC
    AGTGACCGAG GCCTGCAGGA AGGCTATGAA AACAGTCGCT GTTACAATGA GATGGCGCTG
    ATCCGCGTGG TGCAGTCCAT GACCCAGCTG GTGCGGCGGC CCCCCGAGGT CTTTGAGCAG
    GAGATCAGGC AACACTTTAG CACTGGTGGC TGGCGGCTGG TGAACCGTAT CGAGTCCTGG
    CTGGAAACCC ATGCCCTGCT GGAGAAGGCC CAGGCACTGC CCAACGGGGT GCCCAAGGCC
    AGCAGCTCGC CAGAGCCCCC AGCTGTAGCC GAGCTGTCAG ACTCCGGCCA ACAAGAACCT
    GAGGATGGAG GGCCAGCCCC AGGAGAGGCC TCCCAGGGCT CAGACTCAGA GGGCGGTGCC
    CAGGGCCTGG CCTCAGCTAG CAGGGACCAC ACAGACCAGA CTTCGGAGAC CGCACCAGAC
    GCATCGGTGC CACCCAGTGT GAAACCAAAG AAGCGGAGAA AGAGCTACCG GAGCTTCTTA
    CCTGAGAAGA GTGGCTACCC TGACATCGGC TTCCCCCTCT TCCCACTTTC CAAGGGTTTC
    ATCAAGAGCA TCCGGGGTGT CCTGACGCAG TTCCGGGCTG CCCTGCTAGA GGCAGGCATG
    CCGGAGTGCA CAGAGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.