nup188 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup188
Entrez Gene ID	100135081
Full Name	nucleoporin 188kDa
General protein information	Preferred Names nucleoporin 188kDa Names nucleoporin NUP188 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967947.3	XP_012823401.1	nucleoporin NUP188 homolog isoform X1
XM_012967947.2	XP_012823401.1	nucleoporin NUP188 homolog isoform X1
NM_001113820.1	NP_001107292.1	nucleoporin NUP188 homolog
XM_018096135.1	XP_017951624.1	nucleoporin NUP188 homolog isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NUP188	NP_056169.1
Canis lupus familiaris (dog)	NUP188	XP_005625319.1
Mus musculus (house mouse)	Nup188	NP_938046.2
Rattus norvegicus (Norway rat)	Nup188	XP_006233967.1
	NUP188	XP_002800020.1
Bos taurus (cattle)	NUP188	NP_001095308.1
Danio rerio (zebrafish)	nup188	XP_001923018.1
Gallus gallus (chicken)	NUP188	NP_001025861.1
Pan troglodytes (chimpanzee)	NUP188	XP_520414.3
Xenopus tropicalis (tropical clawed frog)	nup188	NP_001107292.1

Gene Ontology
Bibliography

Related articles in PubMed

Rare copy number variations in congenital heart disease patients identify unique genes in left-right patterning.
Fakhro KA, Choi M, Ware SM, Belmont JW, Towbin JA, Lifton RP, Khokha MK, Brueckner M
Proceedings of the National Academy of Sciences of the United States of America108(7)2915-20(2011 Feb)

The genome of the Western clawed frog Xenopus tropicalis.
Hellsten U, Harland RM, Gilchrist MJ, Hendrix D, Jurka J, Kapitonov V, Ovcharenko I, Putnam NH, Shu S, Taher L, Blitz IL, Blumberg B, Dichmann DS, Dubchak I, Amaya E, Detter JC, Fletcher R, Gerhard DS, Goodstein D, Graves T, Grigoriev IV, Grimwood J, Kawashima T, Lindquist E, Lucas SM, Mead PE, Mitros T, Ogino H, Ohta Y, Poliakov AV, Pollet N, Robert J, Salamov A, Sater AK, Schmutz J, Terry A, Vize PD, Warren WC, Wells D, Wills A, Wilson RK, Zimmerman LB, Zorn AM, Grainger R, Grammer T, Khokha MK, Richardson PM, Rokhsar DS
Science (New York, N.Y.)328(5978)633-6(2010 Apr)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nup188 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup188 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa31153	XM_012967947.3 Latest version!	Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa45963	XM_018096135.1 Latest version!	Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa01174	NM_001113820.1 Latest version!	Xenopus tropicalis nucleoporin 188kDa (nup188), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OXa31153	XM_012967947.2	Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup188 ( XM_012967947.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823401.1 Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31153
Clone ID Related Accession (Same CDS sequence)	XM_012967947.3 , XM_012967947.2
Accession Version	XM_012967947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nucleoporin NUP188 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGGGTGTCT GTACCGGAGG ACCAGCCGAG AGTTATGGAC CATTCTGCTT 
GGACGCTCTT CCATGAAAGA TGGGCATCAG ATCAAAGCAG AGCTGGACAG ATATGGAGAC 
CGGCTCCTGC AGGGACTGGC ATACTACAGG CCCCCCAGCA CAAGCTCCAC AGGCAAAGTG 
AAAGCCAATA AGAATCTGTC CCCTGCCCTA CAGGAGCTTG GTTTGCGACT CAGCAAATTT 
CTGGGCCTGG ATGAAGAGCA GAGCGTGGAG CTGTTACAGA CTTACCTGCT GTATGATTAC 
AGAGGGACAC AGGAATCTGT AAAGGGTGTG CCACAAGATG AGAGGCAGAG TCAAGCACTG 
ATGCTGAAGA TGGTAGATTA CTACTATGAA GAGAGAATTT CCCTGCTGCG CTGTGTCCTG 
TACATTCTGA ATTATTTCCA GGATGACAAG CATCCCTATA GCGCAGAATT CTCCAAATGT 
GTGGAAATGA TGGAGCAGAA GGAATTATTC GGCAAGTACC TGAAGCAGTT TGAAAGCCTC 
TGCAGGGAGG AGGCCCCAAC ATGGGAAACA CATGGAAACT TCATGACGGA GCGCCAGGTG 
TCCCGGTGGT TTGTGCAGCG ATTGCGGGAG CAGGCCATGC TCTTGGAGAT CATATTCCTA 
TATTTTGCCT GTTTTGCTGC TTCCCCATCC GACCTGCTAG CACTGACCAA ACTGTTCAAG 
GAGCAAGGCT TCGGGTGCAG GCTGCAAAAC CGCCACCTTG TAGAACCATC AATGGACCCT 
CTGGTGGAGA GAATCGGGTA CTTCTCTGTT TTAATCTTTT TGGAAGCGCT AGACATTGAA 
ACTCTTATGA CCTGCTCTCT CAGTGATAAA ACAGAGCAGC ATCCATTTTC TTCTGAGGAG 
CAGGTCTGCA AGGAGATGGA CAGTGTTTTG GTTACGCTGG GAGACGCCCC ACACCATGGA 
CCTGTGCTGC TGGCCTGGGC CCTGCTGCGT TTTGCTCTGA ATGCTGACAA GGTTACCCCC 
ACTGTCCGTA AAATGGGCAG CACCGCCATC CAGCTCCATA TCTTTCAGTA CTTAACCCGA 
ATGCTTCAAT CTCTTAGAAG TGGGGAGAAT AATTGTACGA CCAGCACGGC TTGTTTGTGT 
GTCTACACCT TCCTGACTTT TGTGCTCACA AACCTAGAGG AACAAGTCTT ACAGAGCCAG 
CAGGATCTTG TAGACACAGC TTTCCAGGTG TTTGCTGCTC CGAATCTGCC TGATCTGTTC 
TGGAACATGG AACCCACAGC AGGCCTGGGG ATCCTTCTGG ACAGCGTGGT TGGGATGTTC 
CCCTTTCACC TCTCGCCTCT CCTGAAGCTT TTCACTGCGC TTGTCTCCAA GTCTTCTGCA 
AAGAAGGTGT ACAGTTTTCT AGACCGGATG TCCTCTTACA CAGAGCACTA CCGCCACAAG 
CCTCACGATA TTCTCTCGCA CGAGGATGAG ACACTATGGA AGAGACAGAC CCCTAAAGTG 
CTGTATGCAC TAGGACTGGG ACAAACAAAC TTGCGCATCC CTCAAGGGAC CATAGGGCAG 
GTGATGGCCG ATGAACATGG CTTCCTGGTC CGCTGGGAGT ATTCCTACAG CTGCTGGACT 
CTGTTTACCT GTGAGATCGA GATGCTGCTG CATGTTGTGT CCACAGCTGA TGTGATCCAC 
CAGTGCCAAA GGGTGAAACC CATCATTGAC CTAGTTCATA AGGTTATCAG CACAGACTTG 
TCCATAGCTG ACTGTCTTCT GCCCATCACA TCCCGGATTT ACATGCTCCT ACAGAGGTTA 
ACGACAGTAA TGAACCCGCC GATGGATTTC CTCTCTTCTT GTGTTGACTG CCTCGCTGCT 
TTAGCCACAC GTATGCCTGC AAAGGTTTGG ATAGACCTAC GACACACAGG GTTCTTGCCC 
TTTGCAGCAA ATCCTGTCTC TGGGCACATC ATTAGCACTG AAGGGATGAA TGCTGGCGGT 
TACGGCTCAC TTTTCGGCAT TGAGCAGTCA CAAGGGGAAT ACAGCATCAC GCTCTCTTTT 
CTCCGTCTGG TCACCACCTT AGTGAAGGGG CAGTTGGGAA GCACCCAAAG CCAGGGCCTT 
GTGCCCTGTA TCCTGTTTGT GCTGCGTGAA ATGCTTCCTA ACTACCACAG GTGGCGCTAT 
AATAGTCACG GCGTGAGAGA ACAACTTGGC TTCCAGATCC TGAGTTTGAT TCATGCAATC 
CTCAACTTAT CCCCTGAGGA AGAGGAGAGC ACCAGCACCC CAAACTTGCA GTCCCTGTGC 
ATCTTCAGCC TGACAAACAC TGAGGCAGGA CAGGCGGTCA TTAACATCAT GGGGGTCGGA 
GTGGACACGC TCAATGCGGT CATGCTGACT CAGGCTGGGA GCAGTGGCAC AGAGGGCCAA 
GGGCAGATGC TCATGCAAAC CATCAAACTG GCTTTTTCCA TCACCAATAA TGTGATCAGA 
CTGAAGCCCC TTTCTAGTGC CATCTCGCCA CTGGAGCATG CACTCACTCA GCACGGAGCT 
CATGGGAACA ATCTAATTGC TGTCCTGGCC AAATATATTT ATCACAAATA TGATCCGTCG 
CTGCCCCGGC TGGCCATCCA GCTGCTGAAA CGCCTGGCCA TGGTGGCACC TATGTCAGTG 
TACGCCTGTT TGGGCAGTGA TGCAGCCGCC ATTAGGGATG CCTTCCTGAG CCGCCTGCGG 
GATAACATTG AGGACATGCA GATCAAGATT ATGATATTGG AATTCCTTAC TGTTGCCGTG 
GAGACGCAGC CTGGACTCAT TGAGCTTTTC CTCAATTTAG AGGTGAAGGA CACAAACGAG 
GGCTCAAAGG AGTACAGCCT CGGGGAGTGG AGCTGTTTGC AGGTTGTGCT GAAGCTTATT 
GATTCCCAAG ATCCCGAGAG CTCCTGGGGA GCCCCTCTGC TCCACCGCTC GGCCATCGCT 
TTCCTCCACG CCCTTTGGCA GGATCGGCGG GACAGCGCCA TGACTGTGCT CAGAACCAAG 
CCCAACTTCT GGGAGAACCT GACAAGCCCC CTGTTTGGGA CACTGGCGTT TCCATCGGAA 
TCCTCAGAGC TGAGCATCCT GGAGACCTGC GCTTTCATAA TGAAAATTAT TTGCCTCGAG 
ATTTACTATG CAGTGAGGGG TTCTCTTGGT GACTCCCTGA AAAAGATCCT AAAGAAATTC 
TCTGAGGAAG AGCGATTCAC TTACTGGTCT AACTACGTAC ATTCCTTGGT GTGCCAGGTG 
GCTGAGAGTG AGGGGGCCTG TAACTCGCTC ACTGAGTATC AGCAGCTGCT GTCAGCTTGG 
CGGATGTTTC TCATGGTCGC CACGCACAAT GCAGATGTAA TGCACCTGAC CAATCCTGAG 
GTCCGACAGA AGCTATTTAA GGATGTCTTG GGCGGCACTC AGGCTCTGCT TCTTGTTCCC 
AGATCCATGA CTTGCCTGCA CCTCGGCTCC ATGCTTTGCA CTGTGATGAT CATCTTACTC 
AGGAGGTGGA AAAGTGACCT AGCAGCGCCT GAGGACATTC TCAGCTCTCT GACACAGATT 
CTGGAGGGGG TCCTGCAGAA AGATCAGCAG CTGGTGGAGA AGACCAAAGC CCGAGTCTTT 
GCCGCCCTCA TCTCTGCGCT AGAGATAAAG CCAATGAAAG CCAGTGAGAT CCCCCAGTAC 
CCCCAATTGG TGCTCAATGT GTGCGAGACC CTGCAGGAAG AAGTGGTGTT TCTTGTGGAT 
CACACACGCC AAGAGGTCCC CGCTAACGAC GCATCCGAGG ACAAGGACAG CATGGAGACC 
GAGGACACAG GCCGGATCCG GCAGAAGGAC CAGCGTGATG GAGTGTGCGT GCTGGGGCTG 
CATTTGGCCA AGGGACTGTG TGAGGCGGAC GAGGAGGGTG ACCAGTGGCA GCAGGTACTC 
AGGAAGCTTC CAGTTCTGCC CATGCTCTTC AGTGCCCTGG AGGTCAGCCT GCGCATCAAG 
CAGAATCTCC ACTTCTGTGA AGCCATTCTC CACTTCCTGT TCACTCTAGC AAAAACACAC 
CAGGGAGCCG CTGCCATGGC AGGTGCTGGC GTCACTCAGA CTGTGTGTTT GCCTCTTCTT 
AGCGTGTACC AACTGAGTTC CAATGGGGCC AGTACGGCTC AGCCAGCTCT GTCGCTCCGC 
AAATCCCTGG ATGCCCCTTC CTGGCCTGGG GTCTACCGAC TTACTGTGTC ACTCATGGAG 
CGCCTGCTGA AGACCTTGAG GTACAACTTC CTGACCGAGG CGCTGGACTT TGTAGGAGTT 
CATCAGGAAC GGATTCTACA GTGTCTGGGC GCTGTGCGCA CCGTACCGAG CCTGGCCTGT 
TTGGAAGAGG CAGATCATAC GGTGGGCTTT CTGCTGCAGC TTTCCAACTT CACCAAAGAG 
TGGCATTTCC ACCTCCCGCA GCTGATTAAG GATGTTCAGA TAAAGAATGG AGAAACCATG 
TCCTCCACTG CAACCCCTCG TGGGCAGCGC ACTCCCCAGA CGCCATCTAA GCAGCCGACA 
GCAGAGAGTG AGGCGCTGGA GCTGAGGCAA CTGCGTTCTG TTCAGCACAG CCTCCTGAAA 
ATTTTGGGCA AAACTCTGGC CACCCTGCGA GCATTTACCC CTGACCTCTG CCAAATCCTC 
CAGGTTCAGC CTCTGGACCT GGCCCAATAT AATCTCCTGT TTGCACTGAG TTTCACCACA 
CCAGCTTTTG ATGCAGACGT TACCCCTTCC TTTGGGACCC TTTTAGCTAC CGTGAATGTA 
ACGCTGAGCA TGCTGGGAGA GATGGATAAA AAGAAAGATC ACCCCCTCGG TCAAGTACTG 
GGAGAGACCA ACAGCACAGT AGACAACAAG AACATCAAGT CTTTGCTGCT CTTCATTATG 
GAGAACTGCT TCTACCTGTT GATATCACAA GCTGTGCGTT ACCTTCGGGA CCCTTCCGTT 
CACCCCCGGG ACAAGCAGCG CATGAAGCAA GAACTAAGCT CGGAGCTGAG TACCCTGCTG 
TCCAGCCTGT CTCGTTACTT CCGGAGAGGT GGCCCCTCGT CTCCTGCCGG CGGCCTCATG 
CCCTCCCCTC AGCCTAAGGG TGCATCGGCT GCAAAGGTGG TCCCTGAAGC CCAGGAGCCC 
CTCATCCAGC TGGTACAAGC CTTTGTGCGC CATGTACAGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823401.1
CDS	59..5203
Translation	MAAAGCLYRRTSRELWTILLGRSSMKDGHQIKAELDRYGDRLLQGLAYYRPPSTSSTGKVKANKNLSPALQELGLRLSKFLGLDEEQSVELLQTYLLYDYRGTQESVKGVPQDERQSQALMLKMVDYYYEERISLLRCVLYILNYFQDDKHPYSAEFSKCVEMMEQKELFGKYLKQFESLCREEAPTWETHGNFMTERQVSRWFVQRLREQAMLLEIIFLYFACFAASPSDLLALTKLFKEQGFGCRLQNRHLVEPSMDPLVERIGYFSVLIFLEALDIETLMTCSLSDKTEQHPFSSEEQVCKEMDSVLVTLGDAPHHGPVLLAWALLRFALNADKVTPTVRKMGSTAIQLHIFQYLTRMLQSLRSGENNCTTSTACLCVYTFLTFVLTNLEEQVLQSQQDLVDTAFQVFAAPNLPDLFWNMEPTAGLGILLDSVVGMFPFHLSPLLKLFTALVSKSSAKKVYSFLDRMSSYTEHYRHKPHDILSHEDETLWKRQTPKVLYALGLGQTNLRIPQGTIGQVMADEHGFLVRWEYSYSCWTLFTCEIEMLLHVVSTADVIHQCQRVKPIIDLVHKVISTDLSIADCLLPITSRIYMLLQRLTTVMNPPMDFLSSCVDCLAALATRMPAKVWIDLRHTGFLPFAANPVSGHIISTEGMNAGGYGSLFGIEQSQGEYSITLSFLRLVTTLVKGQLGSTQSQGLVPCILFVLREMLPNYHRWRYNSHGVREQLGFQILSLIHAILNLSPEEEESTSTPNLQSLCIFSLTNTEAGQAVINIMGVGVDTLNAVMLTQAGSSGTEGQGQMLMQTIKLAFSITNNVIRLKPLSSAISPLEHALTQHGAHGNNLIAVLAKYIYHKYDPSLPRLAIQLLKRLAMVAPMSVYACLGSDAAAIRDAFLSRLRDNIEDMQIKIMILEFLTVAVETQPGLIELFLNLEVKDTNEGSKEYSLGEWSCLQVVLKLIDSQDPESSWGAPLLHRSAIAFLHALWQDRRDSAMTVLRTKPNFWENLTSPLFGTLAFPSESSELSILETCAFIMKIICLEIYYAVRGSLGDSLKKILKKFSEEERFTYWSNYVHSLVCQVAESEGACNSLTEYQQLLSAWRMFLMVATHNADVMHLTNPEVRQKLFKDVLGGTQALLLVPRSMTCLHLGSMLCTVMIILLRRWKSDLAAPEDILSSLTQILEGVLQKDQQLVEKTKARVFAALISALEIKPMKASEIPQYPQLVLNVCETLQEEVVFLVDHTRQEVPANDASEDKDSMETEDTGRIRQKDQRDGVCVLGLHLAKGLCEADEEGDQWQQVLRKLPVLPMLFSALEVSLRIKQNLHFCEAILHFLFTLAKTHQGAAAMAGAGVTQTVCLPLLSVYQLSSNGASTAQPALSLRKSLDAPSWPGVYRLTVSLMERLLKTLRYNFLTEALDFVGVHQERILQCLGAVRTVPSLACLEEADHTVGFLLQLSNFTKEWHFHLPQLIKDVQIKNGETMSSTATPRGQRTPQTPSKQPTAESEALELRQLRSVQHSLLKILGKTLATLRAFTPDLCQILQVQPLDLAQYNLLFALSFTTPAFDADVTPSFGTLLATVNVTLSMLGEMDKKKDHPLGQVLGETNSTVDNKNIKSLLLFIMENCFYLLISQAVRYLRDPSVHPRDKQRMKQELSSELSTLLSSLSRYFRRGGPSSPAGGLMPSPQPKGASAAKVVPEAQEPLIQLVQAFVRHVQR

Target ORF information:

RefSeq Version	XM_012967947.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967947.3

ORF Insert Sequence:

ATGGCGGCGG CCGGGTGTCT GTACCGGAGG ACCAGCCGAG AGTTATGGAC CATTCTGCTT 
GGACGCTCTT CCATGAAAGA TGGGCATCAG ATCAAAGCAG AGCTGGACAG ATATGGAGAC 
CGGCTCCTGC AGGGACTGGC ATACTACAGG CCCCCCAGCA CAAGCTCCAC AGGCAAAGTG 
AAAGCCAATA AGAATCTGTC CCCTGCCCTA CAGGAGCTTG GTTTGCGACT CAGCAAATTT 
CTGGGCCTGG ATGAAGAGCA GAGCGTGGAG CTGTTACAGA CTTACCTGCT GTATGATTAC 
AGAGGGACAC AGGAATCTGT AAAGGGTGTG CCACAAGATG AGAGGCAGAG TCAAGCACTG 
ATGCTGAAGA TGGTAGATTA CTACTATGAA GAGAGAATTT CCCTGCTGCG CTGTGTCCTG 
TACATTCTGA ATTATTTCCA GGATGACAAG CATCCCTATA GCGCAGAATT CTCCAAATGT 
GTGGAAATGA TGGAGCAGAA GGAATTATTC GGCAAGTACC TGAAGCAGTT TGAAAGCCTC 
TGCAGGGAGG AGGCCCCAAC ATGGGAAACA CATGGAAACT TCATGACGGA GCGCCAGGTG 
TCCCGGTGGT TTGTGCAGCG ATTGCGGGAG CAGGCCATGC TCTTGGAGAT CATATTCCTA 
TATTTTGCCT GTTTTGCTGC TTCCCCATCC GACCTGCTAG CACTGACCAA ACTGTTCAAG 
GAGCAAGGCT TCGGGTGCAG GCTGCAAAAC CGCCACCTTG TAGAACCATC AATGGACCCT 
CTGGTGGAGA GAATCGGGTA CTTCTCTGTT TTAATCTTTT TGGAAGCGCT AGACATTGAA 
ACTCTTATGA CCTGCTCTCT CAGTGATAAA ACAGAGCAGC ATCCATTTTC TTCTGAGGAG 
CAGGTCTGCA AGGAGATGGA CAGTGTTTTG GTTACGCTGG GAGACGCCCC ACACCATGGA 
CCTGTGCTGC TGGCCTGGGC CCTGCTGCGT TTTGCTCTGA ATGCTGACAA GGTTACCCCC 
ACTGTCCGTA AAATGGGCAG CACCGCCATC CAGCTCCATA TCTTTCAGTA CTTAACCCGA 
ATGCTTCAAT CTCTTAGAAG TGGGGAGAAT AATTGTACGA CCAGCACGGC TTGTTTGTGT 
GTCTACACCT TCCTGACTTT TGTGCTCACA AACCTAGAGG AACAAGTCTT ACAGAGCCAG 
CAGGATCTTG TAGACACAGC TTTCCAGGTG TTTGCTGCTC CGAATCTGCC TGATCTGTTC 
TGGAACATGG AACCCACAGC AGGCCTGGGG ATCCTTCTGG ACAGCGTGGT TGGGATGTTC 
CCCTTTCACC TCTCGCCTCT CCTGAAGCTT TTCACTGCGC TTGTCTCCAA GTCTTCTGCA 
AAGAAGGTGT ACAGTTTTCT AGACCGGATG TCCTCTTACA CAGAGCACTA CCGCCACAAG 
CCTCACGATA TTCTCTCGCA CGAGGATGAG ACACTATGGA AGAGACAGAC CCCTAAAGTG 
CTGTATGCAC TAGGACTGGG ACAAACAAAC TTGCGCATCC CTCAAGGGAC CATAGGGCAG 
GTGATGGCCG ATGAACATGG CTTCCTGGTC CGCTGGGAGT ATTCCTACAG CTGCTGGACT 
CTGTTTACCT GTGAGATCGA GATGCTGCTG CATGTTGTGT CCACAGCTGA TGTGATCCAC 
CAGTGCCAAA GGGTGAAACC CATCATTGAC CTAGTTCATA AGGTTATCAG CACAGACTTG 
TCCATAGCTG ACTGTCTTCT GCCCATCACA TCCCGGATTT ACATGCTCCT ACAGAGGTTA 
ACGACAGTAA TGAACCCGCC GATGGATTTC CTCTCTTCTT GTGTTGACTG CCTCGCTGCT 
TTAGCCACAC GTATGCCTGC AAAGGTTTGG ATAGACCTAC GACACACAGG GTTCTTGCCC 
TTTGCAGCAA ATCCTGTCTC TGGGCACATC ATTAGCACTG AAGGGATGAA TGCTGGCGGT 
TACGGCTCAC TTTTCGGCAT TGAGCAGTCA CAAGGGGAAT ACAGCATCAC GCTCTCTTTT 
CTCCGTCTGG TCACCACCTT AGTGAAGGGG CAGTTGGGAA GCACCCAAAG CCAGGGCCTT 
GTGCCCTGTA TCCTGTTTGT GCTGCGTGAA ATGCTTCCTA ACTACCACAG GTGGCGCTAT 
AATAGTCACG GCGTGAGAGA ACAACTTGGC TTCCAGATCC TGAGTTTGAT TCATGCAATC 
CTCAACTTAT CCCCTGAGGA AGAGGAGAGC ACCAGCACCC CAAACTTGCA GTCCCTGTGC 
ATCTTCAGCC TGACAAACAC TGAGGCAGGA CAGGCGGTCA TTAACATCAT GGGGGTCGGA 
GTGGACACGC TCAATGCGGT CATGCTGACT CAGGCTGGGA GCAGTGGCAC AGAGGGCCAA 
GGGCAGATGC TCATGCAAAC CATCAAACTG GCTTTTTCCA TCACCAATAA TGTGATCAGA 
CTGAAGCCCC TTTCTAGTGC CATCTCGCCA CTGGAGCATG CACTCACTCA GCACGGAGCT 
CATGGGAACA ATCTAATTGC TGTCCTGGCC AAATATATTT ATCACAAATA TGATCCGTCG 
CTGCCCCGGC TGGCCATCCA GCTGCTGAAA CGCCTGGCCA TGGTGGCACC TATGTCAGTG 
TACGCCTGTT TGGGCAGTGA TGCAGCCGCC ATTAGGGATG CCTTCCTGAG CCGCCTGCGG 
GATAACATTG AGGACATGCA GATCAAGATT ATGATATTGG AATTCCTTAC TGTTGCCGTG 
GAGACGCAGC CTGGACTCAT TGAGCTTTTC CTCAATTTAG AGGTGAAGGA CACAAACGAG 
GGCTCAAAGG AGTACAGCCT CGGGGAGTGG AGCTGTTTGC AGGTTGTGCT GAAGCTTATT 
GATTCCCAAG ATCCCGAGAG CTCCTGGGGA GCCCCTCTGC TCCACCGCTC GGCCATCGCT 
TTCCTCCACG CCCTTTGGCA GGATCGGCGG GACAGCGCCA TGACTGTGCT CAGAACCAAG 
CCCAACTTCT GGGAGAACCT GACAAGCCCC CTGTTTGGGA CACTGGCGTT TCCATCGGAA 
TCCTCAGAGC TGAGCATCCT GGAGACCTGC GCTTTCATAA TGAAAATTAT TTGCCTCGAG 
ATTTACTATG CAGTGAGGGG TTCTCTTGGT GACTCCCTGA AAAAGATCCT AAAGAAATTC 
TCTGAGGAAG AGCGATTCAC TTACTGGTCT AACTACGTAC ATTCCTTGGT GTGCCAGGTG 
GCTGAGAGTG AGGGGGCCTG TAACTCGCTC ACTGAGTATC AGCAGCTGCT GTCAGCTTGG 
CGGATGTTTC TCATGGTCGC CACGCACAAT GCAGATGTAA TGCACCTGAC CAATCCTGAG 
GTCCGACAGA AGCTATTTAA GGATGTCTTG GGCGGCACTC AGGCTCTGCT TCTTGTTCCC 
AGATCCATGA CTTGCCTGCA CCTCGGCTCC ATGCTTTGCA CTGTGATGAT CATCTTACTC 
AGGAGGTGGA AAAGTGACCT AGCAGCGCCT GAGGACATTC TCAGCTCTCT GACACAGATT 
CTGGAGGGGG TCCTGCAGAA AGATCAGCAG CTGGTGGAGA AGACCAAAGC CCGAGTCTTT 
GCCGCCCTCA TCTCTGCGCT AGAGATAAAG CCAATGAAAG CCAGTGAGAT CCCCCAGTAC 
CCCCAATTGG TGCTCAATGT GTGCGAGACC CTGCAGGAAG AAGTGGTGTT TCTTGTGGAT 
CACACACGCC AAGAGGTCCC CGCTAACGAC GCATCCGAGG ACAAGGACAG CATGGAGACC 
GAGGACACAG GCCGGATCCG GCAGAAGGAC CAGCGTGATG GAGTGTGCGT GCTGGGGCTG 
CATTTGGCCA AGGGACTGTG TGAGGCGGAC GAGGAGGGTG ACCAGTGGCA GCAGGTACTC 
AGGAAGCTTC CAGTTCTGCC CATGCTCTTC AGTGCCCTGG AGGTCAGCCT GCGCATCAAG 
CAGAATCTCC ACTTCTGTGA AGCCATTCTC CACTTCCTGT TCACTCTAGC AAAAACACAC 
CAGGGAGCCG CTGCCATGGC AGGTGCTGGC GTCACTCAGA CTGTGTGTTT GCCTCTTCTT 
AGCGTGTACC AACTGAGTTC CAATGGGGCC AGTACGGCTC AGCCAGCTCT GTCGCTCCGC 
AAATCCCTGG ATGCCCCTTC CTGGCCTGGG GTCTACCGAC TTACTGTGTC ACTCATGGAG 
CGCCTGCTGA AGACCTTGAG GTACAACTTC CTGACCGAGG CGCTGGACTT TGTAGGAGTT 
CATCAGGAAC GGATTCTACA GTGTCTGGGC GCTGTGCGCA CCGTACCGAG CCTGGCCTGT 
TTGGAAGAGG CAGATCATAC GGTGGGCTTT CTGCTGCAGC TTTCCAACTT CACCAAAGAG 
TGGCATTTCC ACCTCCCGCA GCTGATTAAG GATGTTCAGA TAAAGAATGG AGAAACCATG 
TCCTCCACTG CAACCCCTCG TGGGCAGCGC ACTCCCCAGA CGCCATCTAA GCAGCCGACA 
GCAGAGAGTG AGGCGCTGGA GCTGAGGCAA CTGCGTTCTG TTCAGCACAG CCTCCTGAAA 
ATTTTGGGCA AAACTCTGGC CACCCTGCGA GCATTTACCC CTGACCTCTG CCAAATCCTC 
CAGGTTCAGC CTCTGGACCT GGCCCAATAT AATCTCCTGT TTGCACTGAG TTTCACCACA 
CCAGCTTTTG ATGCAGACGT TACCCCTTCC TTTGGGACCC TTTTAGCTAC CGTGAATGTA 
ACGCTGAGCA TGCTGGGAGA GATGGATAAA AAGAAAGATC ACCCCCTCGG TCAAGTACTG 
GGAGAGACCA ACAGCACAGT AGACAACAAG AACATCAAGT CTTTGCTGCT CTTCATTATG 
GAGAACTGCT TCTACCTGTT GATATCACAA GCTGTGCGTT ACCTTCGGGA CCCTTCCGTT 
CACCCCCGGG ACAAGCAGCG CATGAAGCAA GAACTAAGCT CGGAGCTGAG TACCCTGCTG 
TCCAGCCTGT CTCGTTACTT CCGGAGAGGT GGCCCCTCGT CTCCTGCCGG CGGCCTCATG 
CCCTCCCCTC AGCCTAAGGG TGCATCGGCT GCAAAGGTGG TCCCTGAAGC CCAGGAGCCC 
CTCATCCAGC TGGTACAAGC CTTTGTGCGC CATGTACAGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup188 ( XM_018096135.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951624.1 Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X2, mRNA.

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CloneID	OXa45963
Clone ID Related Accession (Same CDS sequence)	XM_018096135.1
Accession Version	XM_018096135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nucleoporin NUP188 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT CTGCTTGGAC GCTCTTCCAT GAAAGATGGG CATCAGATCA AAGCAGAGCT 
GGACAGATAT GGAGACCGGC TCCTGCAGGG ACTGGCATAC TACAGGCCCC CCAGGGCCTG 
GATGAAGAGC AGAGCGTGGA GCTGTTACAG ACTTACCTGC TGTATGATTA CAGAGGGACA 
CAGGAATCTG TAAAGGGTGT GCCACAAGAT GAGAGGCAGA GTCAAGCACT GATGCTGAAG 
ATGGTAGATT ACTACTATGA AGAGAGAATT TCCCTGCTGC GCTGTGTCCT GTACATTCTG 
AATTATTTCC AGGATGACAA GCATCCCTAT AGCGCAGAAT TCTCCAAATG TGTGGAAATG 
ATGGAGCAGA AGGAATTATT CGGCAAGTAC CTGAAGCAGT TTGAAAGCCT CTGCAGGGAG 
GAGGCCCCAA CATGGGAAAC ACATGGAAAC TTCATGACGG AGCGCCAGGT GTCCCGGTGG 
TTTGTGCAGC GATTGCGGGA GCAGGCCATG CTCTTGGAGA TCATATTCCT ATATTTTGCC 
TGTTTTGCTG CTTCCCCATC CGACCTGCTA GCACTGACCA AACTGTTCAA GGAGCAAGGC 
TTCGGGTGCA GGCTGCAAAA CCGCCACCTT GTAGAACCAT CAATGGACCC TCTGGTGGAG 
AGAATCGGGT ACTTCTCTGT TTTAATCTTT TTGGAAGCGC TAGACATTGA AACTCTTATG 
ACCTGCTCTC TCAGTGATAA AACAGAGCAG CATCCATTTT CTTCTGAGGA GCAGGTCTGC 
AAGGAGATGG ACAGTGTTTT GGTTACGCTG GGAGACGCCC CACACCATGG ACCTGTGCTG 
CTGGCCTGGG CCCTGCTGCG TTTTGCTCTG AATGCTGACA AGGTTACCCC CACTGTCCGT 
AAAATGGGCA GCACCGCCAT CCAGCTCCAT ATCTTTCAGT ACTTAACCCG AATGCTTCAA 
TCTCTTAGAA GTGGGGAGAA TAATTGTACG ACCAGCACGG CTTGTTTGTG TGTCTACACC 
TTCCTGACTT TTGTGCTCAC AAACCTAGAG GAACAAGTCT TACAGAGCCA GCAGGATCTT 
GTAGACACAG CTTTCCAGGT GTTTGCTGCT CCGAATCTGC CTGATCTGTT CTGGAACATG 
GAACCCACAG CAGGCCTGGG GATCCTTCTG GACAGCGTGG TTGGGATGTT CCCCTTTCAC 
CTCTCGCCTC TCCTGAAGCT TTTCACTGCG CTTGTCTCCA AGTCTTCTGC AAAGAAGGTG 
TACAGTTTTC TAGACCGGAT GTCCTCTTAC ACAGAGCACT ACCGCCACAA GCCTCACGAT 
ATTCTCTCGC ACGAGGATGA GACACTATGG AAGAGACAGA CCCCTAAAGT GCTGTATGCA 
CTAGGACTGG GACAAACAAA CTTGCGCATC CCTCAAGGGA CCATAGGGCA GGTGATGGCC 
GATGAACATG GCTTCCTGGT CCGCTGGGAG TATTCCTACA GCTGCTGGAC TCTGTTTACC 
TGTGAGATCG AGATGCTGCT GCATGTTGTG TCCACAGCTG ATGTGATCCA CCAGTGCCAA 
AGGGTGAAAC CCATCATTGA CCTAGTTCAT AAGGTTATCA GCACAGACTT GTCCATAGCT 
GACTGTCTTC TGCCCATCAC ATCCCGGATT TACATGCTCC TACAGAGGTT AACGACAGTA 
ATGAACCCGC CGATGGATTT CCTCTCTTCT TGTGTTGACT GCCTCGCTGC TTTAGCCACA 
CGTATGCCTG CAAAGGTTTG GATAGACCTA CGACACACAG GGTTCTTGCC CTTTGCAGCA 
AATCCTGTCT CTGGGCACAT CATTAGCACT GAAGGGATGA ATGCTGGCGG TTACGGCTCA 
CTTTTCGGCA TTGAGCAGTC ACAAGGGGAA TACAGCATCA CGCTCTCTTT TCTCCGTCTG 
GTCACCACCT TAGTGAAGGG GCAGTTGGGA AGCACCCAAA GCCAGGGCCT TGTGCCCTGT 
ATCCTGTTTG TGCTGCGTGA AATGCTTCCT AACTACCACA GGTGGCGCTA TAATAGTCAC 
GGCGTGAGAG AACAACTTGG CTTCCAGATC CTGAGTTTGA TTCATGCAAT CCTCAACTTA 
TCCCCTGAGG AAGAGGAGAG CACCAGCACC CCAAACTTGC AGTCCCTGTG CATCTTCAGC 
CTGACAAACA CTGAGGCAGG ACAGGCGGTC ATTAACATCA TGGGGGTCGG AGTGGACACG 
CTCAATGCGG TCATGCTGAC TCAGGCTGGG AGCAGTGGCA CAGAGGGCCA AGGGCAGATG 
CTCATGCAAA CCATCAAACT GGCTTTTTCC ATCACCAATA ATGTGATCAG ACTGAAGCCC 
CTTTCTAGTG CCATCTCGCC ACTGGAGCAT GCACTCACTC AGCACGGAGC TCATGGGAAC 
AATCTAATTG CTGTCCTGGC CAAATATATT TATCACAAAT ATGATCCGTC GCTGCCCCGG 
CTGGCCATCC AGCTGCTGAA ACGCCTGGCC ATGGTGGCAC CTATGTCAGT GTACGCCTGT 
TTGGGCAGTG ATGCAGCCGC CATTAGGGAT GCCTTCCTGA GCCGCCTGCG GGATAACATT 
GAGGACATGC AGATCAAGAT TATGATATTG GAATTCCTTA CTGTTGCCGT GGAGACGCAG 
CCTGGACTCA TTGAGCTTTT CCTCAATTTA GAGGTGAAGG ACACAAACGA GGGCTCAAAG 
GAGTACAGCC TCGGGGAGTG GAGCTGTTTG CAGGTTGTGC TGAAGCTTAT TGATTCCCAA 
GATCCCGAGA GCTCCTGGGG AGCCCCTCTG CTCCACCGCT CGGCCATCGC TTTCCTCCAC 
GCCCTTTGGC AGGATCGGCG GGACAGCGCC ATGACTGTGC TCAGAACCAA GCCCAACTTC 
TGGGAGAACC TGACAAGCCC CCTGTTTGGG ACACTGGCGT TTCCATCGGA ATCCTCAGAG 
CTGAGCATCC TGGAGACCTG CGCTTTCATA ATGAAAATTA TTTGCCTCGA GATTTACTAT 
GCAGTGAGGG GTTCTCTTGG TGACTCCCTG AAAAAGATCC TAAAGAAATT CTCTGAGGAA 
GAGCGATTCA CTTACTGGTC TAACTACGTA CATTCCTTGG TGTGCCAGGT GGCTGAGAGT 
GAGGGGGCCT GTAACTCGCT CACTGAGTAT CAGCAGCTGC TGTCAGCTTG GCGGATGTTT 
CTCATGGTCG CCACGCACAA TGCAGATGTA ATGCACCTGA CCAATCCTGA GGTCCGACAG 
AAGCTATTTA AGGATGTCTT GGGCGGCACT CAGGCTCTGC TTCTTGTTCC CAGATCCATG 
ACTTGCCTGC ACCTCGGCTC CATGCTTTGC ACTGTGATGA TCATCTTACT CAGGAGGTGG 
AAAAGTGACC TAGCAGCGCC TGAGGACATT CTCAGCTCTC TGACACAGAT TCTGGAGGGG 
GTCCTGCAGA AAGATCAGCA GCTGGTGGAG AAGACCAAAG CCCGAGTCTT TGCCGCCCTC 
ATCTCTGCGC TAGAGATAAA GCCAATGAAA GCCAGTGAGA TCCCCCAGTA CCCCCAATTG 
GTGCTCAATG TGTGCGAGAC CCTGCAGGAA GAAGTGGTGT TTCTTGTGGA TCACACACGC 
CAAGAGGTCC CCGCTAACGA CGCATCCGAG GACAAGGACA GCATGGAGAC CGAGGACACA 
GGCCGGATCC GGCAGAAGGA CCAGCGTGAT GGAGTGTGCG TGCTGGGGCT GCATTTGGCC 
AAGGGACTGT GTGAGGCGGA CGAGGAGGGT GACCAGTGGC AGCAGGTACT CAGGAAGCTT 
CCAGTTCTGC CCATGCTCTT CAGTGCCCTG GAGGTCAGCC TGCGCATCAA GCAGAATCTC 
CACTTCTGTG AAGCCATTCT CCACTTCCTG TTCACTCTAG CAAAAACACA CCAGGGAGCC 
GCTGCCATGG CAGGTGCTGG CGTCACTCAG ACTGTGTGTT TGCCTCTTCT TAGCGTGTAC 
CAACTGAGTT CCAATGGGGC CAGTACGGCT CAGCCAGCTC TGTCGCTCCG CAAATCCCTG 
GATGCCCCTT CCTGGCCTGG GGTCTACCGA CTTACTGTGT CACTCATGGA GCGCCTGCTG 
AAGACCTTGA GGTACAACTT CCTGACCGAG GCGCTGGACT TTGTAGGAGT TCATCAGGAA 
CGGATTCTAC AGTGTCTGGG CGCTGTGCGC ACCGTACCGA GCCTGGCCTG TTTGGAAGAG 
GCAGATCATA CGGTGGGCTT TCTGCTGCAG CTTTCCAACT TCACCAAAGA GTGGCATTTC 
CACCTCCCGC AGCTGATTAA GGATGTTCAG GTGAATCTCT GTTACCTGTG CCAGGCCTGC 
ACCTCTCTCC TGCACAGCCG CAAGATGCTG CAGCACTACC TACAGATAAA GAATGGAGAA 
ACCATGTCCT CCACTGCAAC CCCTCGTGGG CAGCGCACTC CCCAGACGCC ATCTAAGCAG 
CCGACAGCAG AGAGTGAGGC GCTGGAGCTG AGGCAACTGC GTTCTGTTCA GCACAGCCTC 
CTGAAAATTT TGGGCAAAAC TCTGGCCACC CTGCGAGCAT TTACCCCTGA CCTCTGCCAA 
ATCCTCCAGG TTCAGCCTCT GGACCTGGCC CAATATAATC TCCTGTTTGC ACTGAGTTTC 
ACCACACCAG CTTTTGATGC AGACGTTACC CCTTCCTTTG GGACCCTTTT AGCTACCGTG 
AATGTAACGC TGAGCATGCT GGGAGAGATG GATAAAAAGA AAGATCACCC CCTCGGTCAA 
GTACTGGGAG AGACCAACAG CACAGTAGAC AACAAGAACA TCAAGTCTTT GCTGCTCTTC 
ATTATGGAGA ACTGCTTCTA CCTGTTGATA TCACAAGCTG TGCGTTACCT TCGGGACCCT 
TCCGTTCACC CCCGGGACAA GCAGCGCATG AAGCAAGAAC TAAGCTCGGA GCTGAGTACC 
CTGCTGTCCA GCCTGTCTCG TTACTTCCGG AGAGGTGGCC CCTCGTCTCC TGCCGGCGGC 
CTCATGCCCT CCCCTCAGCC TAAGGGTGCA TCGGCTGCAA AGGTGGTCCC TGAAGCCCAG 
GAGCCCCTCA TCCAGCTGGT ACAAGCCTTT GTGCGCCATG TACAGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951624.1
CDS	97..5187
Translation	MDHSAWTLFHERWASDQSRAGQIWRPAPAGTGILQAPQGLDEEQSVELLQTYLLYDYRGTQESVKGVPQDERQSQALMLKMVDYYYEERISLLRCVLYILNYFQDDKHPYSAEFSKCVEMMEQKELFGKYLKQFESLCREEAPTWETHGNFMTERQVSRWFVQRLREQAMLLEIIFLYFACFAASPSDLLALTKLFKEQGFGCRLQNRHLVEPSMDPLVERIGYFSVLIFLEALDIETLMTCSLSDKTEQHPFSSEEQVCKEMDSVLVTLGDAPHHGPVLLAWALLRFALNADKVTPTVRKMGSTAIQLHIFQYLTRMLQSLRSGENNCTTSTACLCVYTFLTFVLTNLEEQVLQSQQDLVDTAFQVFAAPNLPDLFWNMEPTAGLGILLDSVVGMFPFHLSPLLKLFTALVSKSSAKKVYSFLDRMSSYTEHYRHKPHDILSHEDETLWKRQTPKVLYALGLGQTNLRIPQGTIGQVMADEHGFLVRWEYSYSCWTLFTCEIEMLLHVVSTADVIHQCQRVKPIIDLVHKVISTDLSIADCLLPITSRIYMLLQRLTTVMNPPMDFLSSCVDCLAALATRMPAKVWIDLRHTGFLPFAANPVSGHIISTEGMNAGGYGSLFGIEQSQGEYSITLSFLRLVTTLVKGQLGSTQSQGLVPCILFVLREMLPNYHRWRYNSHGVREQLGFQILSLIHAILNLSPEEEESTSTPNLQSLCIFSLTNTEAGQAVINIMGVGVDTLNAVMLTQAGSSGTEGQGQMLMQTIKLAFSITNNVIRLKPLSSAISPLEHALTQHGAHGNNLIAVLAKYIYHKYDPSLPRLAIQLLKRLAMVAPMSVYACLGSDAAAIRDAFLSRLRDNIEDMQIKIMILEFLTVAVETQPGLIELFLNLEVKDTNEGSKEYSLGEWSCLQVVLKLIDSQDPESSWGAPLLHRSAIAFLHALWQDRRDSAMTVLRTKPNFWENLTSPLFGTLAFPSESSELSILETCAFIMKIICLEIYYAVRGSLGDSLKKILKKFSEEERFTYWSNYVHSLVCQVAESEGACNSLTEYQQLLSAWRMFLMVATHNADVMHLTNPEVRQKLFKDVLGGTQALLLVPRSMTCLHLGSMLCTVMIILLRRWKSDLAAPEDILSSLTQILEGVLQKDQQLVEKTKARVFAALISALEIKPMKASEIPQYPQLVLNVCETLQEEVVFLVDHTRQEVPANDASEDKDSMETEDTGRIRQKDQRDGVCVLGLHLAKGLCEADEEGDQWQQVLRKLPVLPMLFSALEVSLRIKQNLHFCEAILHFLFTLAKTHQGAAAMAGAGVTQTVCLPLLSVYQLSSNGASTAQPALSLRKSLDAPSWPGVYRLTVSLMERLLKTLRYNFLTEALDFVGVHQERILQCLGAVRTVPSLACLEEADHTVGFLLQLSNFTKEWHFHLPQLIKDVQVNLCYLCQACTSLLHSRKMLQHYLQIKNGETMSSTATPRGQRTPQTPSKQPTAESEALELRQLRSVQHSLLKILGKTLATLRAFTPDLCQILQVQPLDLAQYNLLFALSFTTPAFDADVTPSFGTLLATVNVTLSMLGEMDKKKDHPLGQVLGETNSTVDNKNIKSLLLFIMENCFYLLISQAVRYLRDPSVHPRDKQRMKQELSSELSTLLSSLSRYFRRGGPSSPAGGLMPSPQPKGASAAKVVPEAQEPLIQLVQAFVRHVQR

Target ORF information:

RefSeq Version	XM_018096135.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096135.1

ORF Insert Sequence:

ATGGACCATT CTGCTTGGAC GCTCTTCCAT GAAAGATGGG CATCAGATCA AAGCAGAGCT 
GGACAGATAT GGAGACCGGC TCCTGCAGGG ACTGGCATAC TACAGGCCCC CCAGGGCCTG 
GATGAAGAGC AGAGCGTGGA GCTGTTACAG ACTTACCTGC TGTATGATTA CAGAGGGACA 
CAGGAATCTG TAAAGGGTGT GCCACAAGAT GAGAGGCAGA GTCAAGCACT GATGCTGAAG 
ATGGTAGATT ACTACTATGA AGAGAGAATT TCCCTGCTGC GCTGTGTCCT GTACATTCTG 
AATTATTTCC AGGATGACAA GCATCCCTAT AGCGCAGAAT TCTCCAAATG TGTGGAAATG 
ATGGAGCAGA AGGAATTATT CGGCAAGTAC CTGAAGCAGT TTGAAAGCCT CTGCAGGGAG 
GAGGCCCCAA CATGGGAAAC ACATGGAAAC TTCATGACGG AGCGCCAGGT GTCCCGGTGG 
TTTGTGCAGC GATTGCGGGA GCAGGCCATG CTCTTGGAGA TCATATTCCT ATATTTTGCC 
TGTTTTGCTG CTTCCCCATC CGACCTGCTA GCACTGACCA AACTGTTCAA GGAGCAAGGC 
TTCGGGTGCA GGCTGCAAAA CCGCCACCTT GTAGAACCAT CAATGGACCC TCTGGTGGAG 
AGAATCGGGT ACTTCTCTGT TTTAATCTTT TTGGAAGCGC TAGACATTGA AACTCTTATG 
ACCTGCTCTC TCAGTGATAA AACAGAGCAG CATCCATTTT CTTCTGAGGA GCAGGTCTGC 
AAGGAGATGG ACAGTGTTTT GGTTACGCTG GGAGACGCCC CACACCATGG ACCTGTGCTG 
CTGGCCTGGG CCCTGCTGCG TTTTGCTCTG AATGCTGACA AGGTTACCCC CACTGTCCGT 
AAAATGGGCA GCACCGCCAT CCAGCTCCAT ATCTTTCAGT ACTTAACCCG AATGCTTCAA 
TCTCTTAGAA GTGGGGAGAA TAATTGTACG ACCAGCACGG CTTGTTTGTG TGTCTACACC 
TTCCTGACTT TTGTGCTCAC AAACCTAGAG GAACAAGTCT TACAGAGCCA GCAGGATCTT 
GTAGACACAG CTTTCCAGGT GTTTGCTGCT CCGAATCTGC CTGATCTGTT CTGGAACATG 
GAACCCACAG CAGGCCTGGG GATCCTTCTG GACAGCGTGG TTGGGATGTT CCCCTTTCAC 
CTCTCGCCTC TCCTGAAGCT TTTCACTGCG CTTGTCTCCA AGTCTTCTGC AAAGAAGGTG 
TACAGTTTTC TAGACCGGAT GTCCTCTTAC ACAGAGCACT ACCGCCACAA GCCTCACGAT 
ATTCTCTCGC ACGAGGATGA GACACTATGG AAGAGACAGA CCCCTAAAGT GCTGTATGCA 
CTAGGACTGG GACAAACAAA CTTGCGCATC CCTCAAGGGA CCATAGGGCA GGTGATGGCC 
GATGAACATG GCTTCCTGGT CCGCTGGGAG TATTCCTACA GCTGCTGGAC TCTGTTTACC 
TGTGAGATCG AGATGCTGCT GCATGTTGTG TCCACAGCTG ATGTGATCCA CCAGTGCCAA 
AGGGTGAAAC CCATCATTGA CCTAGTTCAT AAGGTTATCA GCACAGACTT GTCCATAGCT 
GACTGTCTTC TGCCCATCAC ATCCCGGATT TACATGCTCC TACAGAGGTT AACGACAGTA 
ATGAACCCGC CGATGGATTT CCTCTCTTCT TGTGTTGACT GCCTCGCTGC TTTAGCCACA 
CGTATGCCTG CAAAGGTTTG GATAGACCTA CGACACACAG GGTTCTTGCC CTTTGCAGCA 
AATCCTGTCT CTGGGCACAT CATTAGCACT GAAGGGATGA ATGCTGGCGG TTACGGCTCA 
CTTTTCGGCA TTGAGCAGTC ACAAGGGGAA TACAGCATCA CGCTCTCTTT TCTCCGTCTG 
GTCACCACCT TAGTGAAGGG GCAGTTGGGA AGCACCCAAA GCCAGGGCCT TGTGCCCTGT 
ATCCTGTTTG TGCTGCGTGA AATGCTTCCT AACTACCACA GGTGGCGCTA TAATAGTCAC 
GGCGTGAGAG AACAACTTGG CTTCCAGATC CTGAGTTTGA TTCATGCAAT CCTCAACTTA 
TCCCCTGAGG AAGAGGAGAG CACCAGCACC CCAAACTTGC AGTCCCTGTG CATCTTCAGC 
CTGACAAACA CTGAGGCAGG ACAGGCGGTC ATTAACATCA TGGGGGTCGG AGTGGACACG 
CTCAATGCGG TCATGCTGAC TCAGGCTGGG AGCAGTGGCA CAGAGGGCCA AGGGCAGATG 
CTCATGCAAA CCATCAAACT GGCTTTTTCC ATCACCAATA ATGTGATCAG ACTGAAGCCC 
CTTTCTAGTG CCATCTCGCC ACTGGAGCAT GCACTCACTC AGCACGGAGC TCATGGGAAC 
AATCTAATTG CTGTCCTGGC CAAATATATT TATCACAAAT ATGATCCGTC GCTGCCCCGG 
CTGGCCATCC AGCTGCTGAA ACGCCTGGCC ATGGTGGCAC CTATGTCAGT GTACGCCTGT 
TTGGGCAGTG ATGCAGCCGC CATTAGGGAT GCCTTCCTGA GCCGCCTGCG GGATAACATT 
GAGGACATGC AGATCAAGAT TATGATATTG GAATTCCTTA CTGTTGCCGT GGAGACGCAG 
CCTGGACTCA TTGAGCTTTT CCTCAATTTA GAGGTGAAGG ACACAAACGA GGGCTCAAAG 
GAGTACAGCC TCGGGGAGTG GAGCTGTTTG CAGGTTGTGC TGAAGCTTAT TGATTCCCAA 
GATCCCGAGA GCTCCTGGGG AGCCCCTCTG CTCCACCGCT CGGCCATCGC TTTCCTCCAC 
GCCCTTTGGC AGGATCGGCG GGACAGCGCC ATGACTGTGC TCAGAACCAA GCCCAACTTC 
TGGGAGAACC TGACAAGCCC CCTGTTTGGG ACACTGGCGT TTCCATCGGA ATCCTCAGAG 
CTGAGCATCC TGGAGACCTG CGCTTTCATA ATGAAAATTA TTTGCCTCGA GATTTACTAT 
GCAGTGAGGG GTTCTCTTGG TGACTCCCTG AAAAAGATCC TAAAGAAATT CTCTGAGGAA 
GAGCGATTCA CTTACTGGTC TAACTACGTA CATTCCTTGG TGTGCCAGGT GGCTGAGAGT 
GAGGGGGCCT GTAACTCGCT CACTGAGTAT CAGCAGCTGC TGTCAGCTTG GCGGATGTTT 
CTCATGGTCG CCACGCACAA TGCAGATGTA ATGCACCTGA CCAATCCTGA GGTCCGACAG 
AAGCTATTTA AGGATGTCTT GGGCGGCACT CAGGCTCTGC TTCTTGTTCC CAGATCCATG 
ACTTGCCTGC ACCTCGGCTC CATGCTTTGC ACTGTGATGA TCATCTTACT CAGGAGGTGG 
AAAAGTGACC TAGCAGCGCC TGAGGACATT CTCAGCTCTC TGACACAGAT TCTGGAGGGG 
GTCCTGCAGA AAGATCAGCA GCTGGTGGAG AAGACCAAAG CCCGAGTCTT TGCCGCCCTC 
ATCTCTGCGC TAGAGATAAA GCCAATGAAA GCCAGTGAGA TCCCCCAGTA CCCCCAATTG 
GTGCTCAATG TGTGCGAGAC CCTGCAGGAA GAAGTGGTGT TTCTTGTGGA TCACACACGC 
CAAGAGGTCC CCGCTAACGA CGCATCCGAG GACAAGGACA GCATGGAGAC CGAGGACACA 
GGCCGGATCC GGCAGAAGGA CCAGCGTGAT GGAGTGTGCG TGCTGGGGCT GCATTTGGCC 
AAGGGACTGT GTGAGGCGGA CGAGGAGGGT GACCAGTGGC AGCAGGTACT CAGGAAGCTT 
CCAGTTCTGC CCATGCTCTT CAGTGCCCTG GAGGTCAGCC TGCGCATCAA GCAGAATCTC 
CACTTCTGTG AAGCCATTCT CCACTTCCTG TTCACTCTAG CAAAAACACA CCAGGGAGCC 
GCTGCCATGG CAGGTGCTGG CGTCACTCAG ACTGTGTGTT TGCCTCTTCT TAGCGTGTAC 
CAACTGAGTT CCAATGGGGC CAGTACGGCT CAGCCAGCTC TGTCGCTCCG CAAATCCCTG 
GATGCCCCTT CCTGGCCTGG GGTCTACCGA CTTACTGTGT CACTCATGGA GCGCCTGCTG 
AAGACCTTGA GGTACAACTT CCTGACCGAG GCGCTGGACT TTGTAGGAGT TCATCAGGAA 
CGGATTCTAC AGTGTCTGGG CGCTGTGCGC ACCGTACCGA GCCTGGCCTG TTTGGAAGAG 
GCAGATCATA CGGTGGGCTT TCTGCTGCAG CTTTCCAACT TCACCAAAGA GTGGCATTTC 
CACCTCCCGC AGCTGATTAA GGATGTTCAG GTGAATCTCT GTTACCTGTG CCAGGCCTGC 
ACCTCTCTCC TGCACAGCCG CAAGATGCTG CAGCACTACC TACAGATAAA GAATGGAGAA 
ACCATGTCCT CCACTGCAAC CCCTCGTGGG CAGCGCACTC CCCAGACGCC ATCTAAGCAG 
CCGACAGCAG AGAGTGAGGC GCTGGAGCTG AGGCAACTGC GTTCTGTTCA GCACAGCCTC 
CTGAAAATTT TGGGCAAAAC TCTGGCCACC CTGCGAGCAT TTACCCCTGA CCTCTGCCAA 
ATCCTCCAGG TTCAGCCTCT GGACCTGGCC CAATATAATC TCCTGTTTGC ACTGAGTTTC 
ACCACACCAG CTTTTGATGC AGACGTTACC CCTTCCTTTG GGACCCTTTT AGCTACCGTG 
AATGTAACGC TGAGCATGCT GGGAGAGATG GATAAAAAGA AAGATCACCC CCTCGGTCAA 
GTACTGGGAG AGACCAACAG CACAGTAGAC AACAAGAACA TCAAGTCTTT GCTGCTCTTC 
ATTATGGAGA ACTGCTTCTA CCTGTTGATA TCACAAGCTG TGCGTTACCT TCGGGACCCT 
TCCGTTCACC CCCGGGACAA GCAGCGCATG AAGCAAGAAC TAAGCTCGGA GCTGAGTACC 
CTGCTGTCCA GCCTGTCTCG TTACTTCCGG AGAGGTGGCC CCTCGTCTCC TGCCGGCGGC 
CTCATGCCCT CCCCTCAGCC TAAGGGTGCA TCGGCTGCAA AGGTGGTCCC TGAAGCCCAG 
GAGCCCCTCA TCCAGCTGGT ACAAGCCTTT GTGCGCCATG TACAGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup188 ( NM_001113820.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001107292.1 Xenopus tropicalis nucleoporin 188kDa (nup188), mRNA.

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CloneID	OXa01174
Clone ID Related Accession (Same CDS sequence)	NM_001113820.1
Accession Version	NM_001113820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nucleoporin NUP188 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155686.1. ##Evidence-Data-START## Transcript exon combination :: BC155686.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG CCGGGTGTCT GTACCGGAGG ACCAGCCGAG AGTTATGGAC CATTCTGCTT 
GGACGCTCTT CCATGAAAGA TGGGCATCAG ATCAAAGCAG AGCTGGACAG ATATGGAGAC 
CGGCTCCTGC AGGGACTGGC ATACTACAGG CCCCCCAGCA CAAGCTCCAC AGGCAAAGTG 
AAAGCCAATA AGAATCTGTC CCCTGCCCTA CAGGAGCTTG GTTTGCGACT CAGCAAATTT 
CTGGGCCTGG ATGAAGAGCA GAGCGTGGAG CTGTTACAGA CTTACCTGCT GTATGATTAC 
AGAGGGACAC AGGAATCTGT AAAGGGTGTG CCACAAGATG AGAGGCAGAG TCAAGCACTG 
ATGCTGAAGA TGGTAGATTA CTACTATGAA GAGAGAATTT CCCTGCTGCG CTGTGTCCTG 
TACATTCTGA ATTATTTCCA GGATGACAAG CATCCCTATA GCGCAGAATT CTCCAAATGT 
GTGGAAATGA TGGAGCAGAA GGAATTATTC GGCAAGTACC TGAAGCAGTT TGAAAGCCTC 
TGCAGGGAGG AGGCCCCAAC ATGGGAAACA CATGGAAACT TCATGACGGA GCGCCAGGTG 
TCCCGGTGGT TTGTGCAGCG ATTGCGGGAG CAGGCCATGC TCTTGGAGAT CATATTCCTA 
TATTTTGCCT GTTTTGCTGC TTCCCCATCC GACCTGCTAG CACTGACCAA ACTGTTCAAG 
GAGCAAGGCT TCGGGTGCAG GCTGCAAAAC CGCCACCTTG TAGAACCATC AATGGACCCT 
CTGGTGGAGA GAATCGGGTA CTTCTCTGTT TTAATCTTTT TGGAAGCGCT AGACATTGAA 
ACTCTTATGA CCTGCTCTCT CAGTGATAAA ACAGAGCAGC ATCCATTTTC TTCTGAGGAG 
CAGGTCTGCA AGGAGATGGA CAGTGTTTTG GTTACGCTGG GAGACGCCCC ACACCATGGA 
CCTGTGCTGC TGGCCTGGGC CCTGCTGCGT TTTGCTCTGA ATGCTGACAA GGTTACCCCC 
ACTGTCCGTA AAATGGGCAG CACCGCCATC CAGCTCCATA TCTTTCAGTA CTTAACCCGA 
ATGCTTCAAT CTCTTAGAAG TGGGGAGAAT AATTGTACGA CCAGCACGGC TTGTTTGTGT 
GTCTACACCT TCCTGACTTT TGTGCTCACA AACCTAGAGG AACAAGTCTT ACAGAGCCAG 
CAGGATCTTG TAGACACAGC TTTCCAGGTG TTTGCTGCTC CGAATCTGCC TGATCTGTTC 
TGGAACATGG AACCCACAGC AGGCCTGGGG ATCCTTCTGG ACAGCGTGGT TGGGATGTTC 
CCCTTTCACC TCTCGCCTCT CCTGAAGCTT TTCACTGCGC TTGTCTCCAA GTCTTCTGCA 
AAGAAGGTGT ACAGTTTTCT AGACCGGATG TCCTCTTACA CAGAGCACTA CCGCCACAAG 
CCTCACGATA TTCTCTCGCA CGAGGATGAG ACACTATGGA AGAGACAGAC CCCTAAAGTG 
CTGTATGCAC TAGGACTGGG ACAAACAAAC TTGCGCATCC CTCAAGGGAC CATAGGGCAG 
GTGATGGCCG ATGAACATGG CTTCCTGGTC CGCTGGGAGT ATTCCTACAG CTGCTGGACT 
CTGTTTACCT GTGAGATCGA GATGCTGCTG CATGTTGTGT CCACAGCTGA TGTGATCCAC 
CAGTGCCAAA GGGTGAAACC CATCATTGAC CTAGTTCATA AGGTTATCAG CACAGACTTG 
TCCATAGCTG ACTGTCTTCT GCCCATCACA TCCCGGATTT ACATGCTCCT ACAGAGGTTA 
ACGACAGTAA TGAACCCGCC GATGGATTTC CTCTCTTCTT GTGTTGACTG CCTCGCTGCT 
TTAGCCACAC GTATGCCTGC AAAGGTTTGG ATAGACCTAC GACACACAGG GTTCTTGCCC 
TTTGCAGCAA ATCCTGTCTC TGGGCACATC ATTAGCACTG AAGGGATGAA TGCTGGCGGT 
TACGGCTCAC TTTTCGGCAT TGAGCAGTCA CAAGGGGAAT ACAGCATCAC GCTCTCTTTT 
CTCCGTCTGG TCACCACCTT AGTGAAGGGG CAGTTGGGAA GCACCCAAAG CCAGGGCCTT 
GTGCCCTGTA TCCTGTTTGT GCTGCGTGAA ATGCTTCCTA ACTACCACAG GTGGCGCTAT 
AATAGTCACG GCGTGAGAGA ACAACTTGGC TTCCAGATCC TGAGTTTGAT TCATGCAATC 
CTCAACTTAT CCCCTGAGGA AGAGGAGAGC ACCAGCACCC CAAACTTGCA GTCCCTGTGC 
ATCTTCAGCC TGACAAACAC TGAGGCAGGA CAGGCGGTCA TTAACATCAT GGGGGTCGGA 
GTGGACACGC TCAATGCGGT CATGCTGACT CAGGCTGGGA GCAGTGGCAC AGAGGGCCAA 
GGGCAGATGC TCATGCAAAC CATCAAACTG GCTTTTTCCA TCACCAATAA TGTGATCAGA 
CTGAAGCCCC TTTCTAGTGC CATCTCGCCA CTGGAGCATG CACTCACTCA GCACGGAGCT 
CATGGGAACA ATCTAATTGC TGTCCTGGCC AAATATATTT ATCACAAATA TGATCCGTCG 
CTGCCCCGGC TGGCCATCCA GCTGCTGAAA CGCCTGGCCA TGGTGGCACC TATGTCAGTG 
TACGCCTGTT TGGGCAGTGA TGCAGCCGCC ATTAGGGATG CCTTCCTGAG CCGCCTGCGG 
GATAACATTG AGGACATGCA GATCAAGATT ATGATATTGG AATTCCTTAC TGTTGCCGTG 
GAGACGCAGC CTGGACTCAT TGAGCTTTTC CTCAATTTAG AGGTGAAGGA CACAAACGAG 
GGCTCAAAGG AGTACAGCCT CGGGGAGTGG AGCTGTTTGC AGGTTGTGCT GAAGCTTATT 
GATTCCCAAG ATCCCGAGAG CTCCTGGGGA GCCCCTCTGC TCCACCGCTC GGCCATCGCT 
TTCCTCCACG CCCTTTGGCA GGATCGGCGG GACAGCGCCA TGACTGTGCT CAGAACCAAG 
CCCAACTTCT GGGAGAACCT GACAAGCCCC CTGTTTGGGA CACTGGCGTT TCCATCGGAA 
TCCTCAGAGC TGAGCATCCT GGAGACCTGC GCTTTCATAA TGAAAATTAT TTGCCTCGAG 
ATTTACTATG CAGTGAGGGG TTCTCTTGGT GACTCCCTGA AAAAGATCCT AAAGAAATTC 
TCTGAGGAAG AGCGATTCAC TTACTGGTCT AACTACGTAC ATTCCTTGGT GTGCCAGGTG 
GCTGAGAGTG AGGGGGCCTG TAACTCGCTC ACTGAGTATC AGCAGCTGCT GTCAGCTTGG 
CGGATGTTTC TCATGGTCGC CACGCACAAT GCAGATGTAA TGCACCTGAC CAATCCTGAG 
GTCCGACAGA AGCTATTTAA GGATGTCTTG GGCGGCACTC AGGCTCTGCT TCTTGTTCCC 
AGATCCATGA CTTGCCTGCA CCTCGGCTCC ATGCTTTGCA CTGTGATGAT CATCTTACTC 
AGGAGGTGGA AAAGTGACCT AGCAGCGCCT GAGGACATTC TCAGCTCTCT GACACAGATT 
CTGGAGGGGG TCCTGCAGAA AGATCAGCAG CTGGTGGAGA AGACCAAAGC CCGAGTCTTT 
GCCGCCCTCA TCTCTGCGCT AGAGATAAAG CCAATGAAAG CCAGTGAGAT CCCCCAGTAC 
CCCCAATTGG TGCTCAATGT GTGCGAGACC CTGCAGGAAG AAGTGGTGTT TCTTGTGGAT 
CACACACGCC AAGAGGTCCC CGCTAACGAC GCATCCGAGG ACAAGGACAG CATGGAGACC 
GAGGACACAG GCCGGATCCG GCAGAAGGAC CAGCGTGATG GAGTGTGCGT GCTGGGGCTG 
CATTTGGCCA AGGGACTGTG TGAGGCGGAC GAGGAGGGTG ACCAGTGGCA GCAGGTACTC 
AGGAAGCTTC CAGTTCTGCC CATGCTCTTC AGTGCCCTGG AGGTCAGCCT GCGCATCAAG 
CAGAATCTCC ACTTCTGTGA AGCCATTCTC CACTTCCTGT TCACTCTAGC AAAAACACAC 
CAGGGAGCCG CTGCCATGGC AGGTGCTGGC GTCACTCAGA CTGTGTGTTT GCCTCTTCTT 
AGCGTGTACC AACTGAGTTC CAATGGGGCC AGTACGGCTC AGCCAGCTCT GTCGCTCCGC 
AAATCCCTGG ATGCCCCTTC CTGGCCTGGG GTCTACCGAC TTACTGTGTC ACTCATGGAG 
CGCCTGCTGA AGACCTTGAG GTACAACTTC CTGACCGAGG CGCTGGACTT TGTAGGAGTT 
CATCAGGAAC GGATTCTACA GTGTCTGGGC GCTGTGCGCA CCGTACCGAG CCTGGCCTGT 
TTGGAAGAGG CAGATCATAC GGTGGGCTTT CTGCTGCAGC TTTCCAACTT CACCAAAGAG 
TGGCATTTCC ACCTCCCGCA GCTGATTAAG GATGTTCAGG TGAATCTCTG TTACCTGTGC 
CAGGCCTGCA CCTCTCTCCT GCACAGCCGC AAGATGCTGC AGCACTACCT ACAGATAAAG 
AATGGAGAAA CCATGTCCTC CACTGCAACC CCTCGTGGGC AGCGCACTCC CCAGACGCCA 
TCTAAGCAGC CGACAGCAGA GAGTGAGGCG CTGGAGCTGA GGCAACTGCG TTCTGTTCAG 
CACAGCCTCC TGAAAATTTT GGGCAAAACT CTGGCCACCC TGCGAGCATT TACCCCTGAC 
CTCTGCCAAA TCCTCCAGGT TCAGCCTCTG GACCTGGCCC AATATAATCT CCTGTTTGCA 
CTGAGTTTCA CCACACCAGC TTTTGATGCA GACGTTACCC CTTCCTTTGG GACCCTTTTA 
GCTACCGTGA ATGTAACGCT GAGCATGCTG GGAGAGATGG ATAAAAAGAA AGATCACCCC 
CTCGGTCAAG TACTGGGAGA GACCAACAGC ACAGTAGACA ACAAGAACAT CAAGTCTTTG 
CTGCTCTTCA TTATGGAGAA CTGCTTCTAC CTGTTGATAT CACAAGCTGT GCGTTACCTT 
CGGGACCCTT CCGTTCACCC CCGGGACAAG CAGCGCATGA AGCAAGAACT AAGCTCGGAG 
CTGAGTACCC TGCTGTCCAG CCTGTCTCGT TACTTCCGGA GAGGTGGCCC CTCGTCTCCT 
GCCGGCGGCC TCATGCCCTC CCCTCAGCCT AAGGGTGCAT CGGCTGCAAA GGTGGTCCCT 
GAAGCCCAGG AGCCCCTCAT CCAGCTGGTA CAAGCCTTTG TGCGCCATGT ACAGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107292.1
CDS	43..5262
Translation	MAAAGCLYRRTSRELWTILLGRSSMKDGHQIKAELDRYGDRLLQGLAYYRPPSTSSTGKVKANKNLSPALQELGLRLSKFLGLDEEQSVELLQTYLLYDYRGTQESVKGVPQDERQSQALMLKMVDYYYEERISLLRCVLYILNYFQDDKHPYSAEFSKCVEMMEQKELFGKYLKQFESLCREEAPTWETHGNFMTERQVSRWFVQRLREQAMLLEIIFLYFACFAASPSDLLALTKLFKEQGFGCRLQNRHLVEPSMDPLVERIGYFSVLIFLEALDIETLMTCSLSDKTEQHPFSSEEQVCKEMDSVLVTLGDAPHHGPVLLAWALLRFALNADKVTPTVRKMGSTAIQLHIFQYLTRMLQSLRSGENNCTTSTACLCVYTFLTFVLTNLEEQVLQSQQDLVDTAFQVFAAPNLPDLFWNMEPTAGLGILLDSVVGMFPFHLSPLLKLFTALVSKSSAKKVYSFLDRMSSYTEHYRHKPHDILSHEDETLWKRQTPKVLYALGLGQTNLRIPQGTIGQVMADEHGFLVRWEYSYSCWTLFTCEIEMLLHVVSTADVIHQCQRVKPIIDLVHKVISTDLSIADCLLPITSRIYMLLQRLTTVMNPPMDFLSSCVDCLAALATRMPAKVWIDLRHTGFLPFAANPVSGHIISTEGMNAGGYGSLFGIEQSQGEYSITLSFLRLVTTLVKGQLGSTQSQGLVPCILFVLREMLPNYHRWRYNSHGVREQLGFQILSLIHAILNLSPEEEESTSTPNLQSLCIFSLTNTEAGQAVINIMGVGVDTLNAVMLTQAGSSGTEGQGQMLMQTIKLAFSITNNVIRLKPLSSAISPLEHALTQHGAHGNNLIAVLAKYIYHKYDPSLPRLAIQLLKRLAMVAPMSVYACLGSDAAAIRDAFLSRLRDNIEDMQIKIMILEFLTVAVETQPGLIELFLNLEVKDTNEGSKEYSLGEWSCLQVVLKLIDSQDPESSWGAPLLHRSAIAFLHALWQDRRDSAMTVLRTKPNFWENLTSPLFGTLAFPSESSELSILETCAFIMKIICLEIYYAVRGSLGDSLKKILKKFSEEERFTYWSNYVHSLVCQVAESEGACNSLTEYQQLLSAWRMFLMVATHNADVMHLTNPEVRQKLFKDVLGGTQALLLVPRSMTCLHLGSMLCTVMIILLRRWKSDLAAPEDILSSLTQILEGVLQKDQQLVEKTKARVFAALISALEIKPMKASEIPQYPQLVLNVCETLQEEVVFLVDHTRQEVPANDASEDKDSMETEDTGRIRQKDQRDGVCVLGLHLAKGLCEADEEGDQWQQVLRKLPVLPMLFSALEVSLRIKQNLHFCEAILHFLFTLAKTHQGAAAMAGAGVTQTVCLPLLSVYQLSSNGASTAQPALSLRKSLDAPSWPGVYRLTVSLMERLLKTLRYNFLTEALDFVGVHQERILQCLGAVRTVPSLACLEEADHTVGFLLQLSNFTKEWHFHLPQLIKDVQVNLCYLCQACTSLLHSRKMLQHYLQIKNGETMSSTATPRGQRTPQTPSKQPTAESEALELRQLRSVQHSLLKILGKTLATLRAFTPDLCQILQVQPLDLAQYNLLFALSFTTPAFDADVTPSFGTLLATVNVTLSMLGEMDKKKDHPLGQVLGETNSTVDNKNIKSLLLFIMENCFYLLISQAVRYLRDPSVHPRDKQRMKQELSSELSTLLSSLSRYFRRGGPSSPAGGLMPSPQPKGASAAKVVPEAQEPLIQLVQAFVRHVQR

Target ORF information:

RefSeq Version	NM_001113820.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 188kDa (nup188), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113820.1

ORF Insert Sequence:

ATGGCGGCGG CCGGGTGTCT GTACCGGAGG ACCAGCCGAG AGTTATGGAC CATTCTGCTT 
GGACGCTCTT CCATGAAAGA TGGGCATCAG ATCAAAGCAG AGCTGGACAG ATATGGAGAC 
CGGCTCCTGC AGGGACTGGC ATACTACAGG CCCCCCAGCA CAAGCTCCAC AGGCAAAGTG 
AAAGCCAATA AGAATCTGTC CCCTGCCCTA CAGGAGCTTG GTTTGCGACT CAGCAAATTT 
CTGGGCCTGG ATGAAGAGCA GAGCGTGGAG CTGTTACAGA CTTACCTGCT GTATGATTAC 
AGAGGGACAC AGGAATCTGT AAAGGGTGTG CCACAAGATG AGAGGCAGAG TCAAGCACTG 
ATGCTGAAGA TGGTAGATTA CTACTATGAA GAGAGAATTT CCCTGCTGCG CTGTGTCCTG 
TACATTCTGA ATTATTTCCA GGATGACAAG CATCCCTATA GCGCAGAATT CTCCAAATGT 
GTGGAAATGA TGGAGCAGAA GGAATTATTC GGCAAGTACC TGAAGCAGTT TGAAAGCCTC 
TGCAGGGAGG AGGCCCCAAC ATGGGAAACA CATGGAAACT TCATGACGGA GCGCCAGGTG 
TCCCGGTGGT TTGTGCAGCG ATTGCGGGAG CAGGCCATGC TCTTGGAGAT CATATTCCTA 
TATTTTGCCT GTTTTGCTGC TTCCCCATCC GACCTGCTAG CACTGACCAA ACTGTTCAAG 
GAGCAAGGCT TCGGGTGCAG GCTGCAAAAC CGCCACCTTG TAGAACCATC AATGGACCCT 
CTGGTGGAGA GAATCGGGTA CTTCTCTGTT TTAATCTTTT TGGAAGCGCT AGACATTGAA 
ACTCTTATGA CCTGCTCTCT CAGTGATAAA ACAGAGCAGC ATCCATTTTC TTCTGAGGAG 
CAGGTCTGCA AGGAGATGGA CAGTGTTTTG GTTACGCTGG GAGACGCCCC ACACCATGGA 
CCTGTGCTGC TGGCCTGGGC CCTGCTGCGT TTTGCTCTGA ATGCTGACAA GGTTACCCCC 
ACTGTCCGTA AAATGGGCAG CACCGCCATC CAGCTCCATA TCTTTCAGTA CTTAACCCGA 
ATGCTTCAAT CTCTTAGAAG TGGGGAGAAT AATTGTACGA CCAGCACGGC TTGTTTGTGT 
GTCTACACCT TCCTGACTTT TGTGCTCACA AACCTAGAGG AACAAGTCTT ACAGAGCCAG 
CAGGATCTTG TAGACACAGC TTTCCAGGTG TTTGCTGCTC CGAATCTGCC TGATCTGTTC 
TGGAACATGG AACCCACAGC AGGCCTGGGG ATCCTTCTGG ACAGCGTGGT TGGGATGTTC 
CCCTTTCACC TCTCGCCTCT CCTGAAGCTT TTCACTGCGC TTGTCTCCAA GTCTTCTGCA 
AAGAAGGTGT ACAGTTTTCT AGACCGGATG TCCTCTTACA CAGAGCACTA CCGCCACAAG 
CCTCACGATA TTCTCTCGCA CGAGGATGAG ACACTATGGA AGAGACAGAC CCCTAAAGTG 
CTGTATGCAC TAGGACTGGG ACAAACAAAC TTGCGCATCC CTCAAGGGAC CATAGGGCAG 
GTGATGGCCG ATGAACATGG CTTCCTGGTC CGCTGGGAGT ATTCCTACAG CTGCTGGACT 
CTGTTTACCT GTGAGATCGA GATGCTGCTG CATGTTGTGT CCACAGCTGA TGTGATCCAC 
CAGTGCCAAA GGGTGAAACC CATCATTGAC CTAGTTCATA AGGTTATCAG CACAGACTTG 
TCCATAGCTG ACTGTCTTCT GCCCATCACA TCCCGGATTT ACATGCTCCT ACAGAGGTTA 
ACGACAGTAA TGAACCCGCC GATGGATTTC CTCTCTTCTT GTGTTGACTG CCTCGCTGCT 
TTAGCCACAC GTATGCCTGC AAAGGTTTGG ATAGACCTAC GACACACAGG GTTCTTGCCC 
TTTGCAGCAA ATCCTGTCTC TGGGCACATC ATTAGCACTG AAGGGATGAA TGCTGGCGGT 
TACGGCTCAC TTTTCGGCAT TGAGCAGTCA CAAGGGGAAT ACAGCATCAC GCTCTCTTTT 
CTCCGTCTGG TCACCACCTT AGTGAAGGGG CAGTTGGGAA GCACCCAAAG CCAGGGCCTT 
GTGCCCTGTA TCCTGTTTGT GCTGCGTGAA ATGCTTCCTA ACTACCACAG GTGGCGCTAT 
AATAGTCACG GCGTGAGAGA ACAACTTGGC TTCCAGATCC TGAGTTTGAT TCATGCAATC 
CTCAACTTAT CCCCTGAGGA AGAGGAGAGC ACCAGCACCC CAAACTTGCA GTCCCTGTGC 
ATCTTCAGCC TGACAAACAC TGAGGCAGGA CAGGCGGTCA TTAACATCAT GGGGGTCGGA 
GTGGACACGC TCAATGCGGT CATGCTGACT CAGGCTGGGA GCAGTGGCAC AGAGGGCCAA 
GGGCAGATGC TCATGCAAAC CATCAAACTG GCTTTTTCCA TCACCAATAA TGTGATCAGA 
CTGAAGCCCC TTTCTAGTGC CATCTCGCCA CTGGAGCATG CACTCACTCA GCACGGAGCT 
CATGGGAACA ATCTAATTGC TGTCCTGGCC AAATATATTT ATCACAAATA TGATCCGTCG 
CTGCCCCGGC TGGCCATCCA GCTGCTGAAA CGCCTGGCCA TGGTGGCACC TATGTCAGTG 
TACGCCTGTT TGGGCAGTGA TGCAGCCGCC ATTAGGGATG CCTTCCTGAG CCGCCTGCGG 
GATAACATTG AGGACATGCA GATCAAGATT ATGATATTGG AATTCCTTAC TGTTGCCGTG 
GAGACGCAGC CTGGACTCAT TGAGCTTTTC CTCAATTTAG AGGTGAAGGA CACAAACGAG 
GGCTCAAAGG AGTACAGCCT CGGGGAGTGG AGCTGTTTGC AGGTTGTGCT GAAGCTTATT 
GATTCCCAAG ATCCCGAGAG CTCCTGGGGA GCCCCTCTGC TCCACCGCTC GGCCATCGCT 
TTCCTCCACG CCCTTTGGCA GGATCGGCGG GACAGCGCCA TGACTGTGCT CAGAACCAAG 
CCCAACTTCT GGGAGAACCT GACAAGCCCC CTGTTTGGGA CACTGGCGTT TCCATCGGAA 
TCCTCAGAGC TGAGCATCCT GGAGACCTGC GCTTTCATAA TGAAAATTAT TTGCCTCGAG 
ATTTACTATG CAGTGAGGGG TTCTCTTGGT GACTCCCTGA AAAAGATCCT AAAGAAATTC 
TCTGAGGAAG AGCGATTCAC TTACTGGTCT AACTACGTAC ATTCCTTGGT GTGCCAGGTG 
GCTGAGAGTG AGGGGGCCTG TAACTCGCTC ACTGAGTATC AGCAGCTGCT GTCAGCTTGG 
CGGATGTTTC TCATGGTCGC CACGCACAAT GCAGATGTAA TGCACCTGAC CAATCCTGAG 
GTCCGACAGA AGCTATTTAA GGATGTCTTG GGCGGCACTC AGGCTCTGCT TCTTGTTCCC 
AGATCCATGA CTTGCCTGCA CCTCGGCTCC ATGCTTTGCA CTGTGATGAT CATCTTACTC 
AGGAGGTGGA AAAGTGACCT AGCAGCGCCT GAGGACATTC TCAGCTCTCT GACACAGATT 
CTGGAGGGGG TCCTGCAGAA AGATCAGCAG CTGGTGGAGA AGACCAAAGC CCGAGTCTTT 
GCCGCCCTCA TCTCTGCGCT AGAGATAAAG CCAATGAAAG CCAGTGAGAT CCCCCAGTAC 
CCCCAATTGG TGCTCAATGT GTGCGAGACC CTGCAGGAAG AAGTGGTGTT TCTTGTGGAT 
CACACACGCC AAGAGGTCCC CGCTAACGAC GCATCCGAGG ACAAGGACAG CATGGAGACC 
GAGGACACAG GCCGGATCCG GCAGAAGGAC CAGCGTGATG GAGTGTGCGT GCTGGGGCTG 
CATTTGGCCA AGGGACTGTG TGAGGCGGAC GAGGAGGGTG ACCAGTGGCA GCAGGTACTC 
AGGAAGCTTC CAGTTCTGCC CATGCTCTTC AGTGCCCTGG AGGTCAGCCT GCGCATCAAG 
CAGAATCTCC ACTTCTGTGA AGCCATTCTC CACTTCCTGT TCACTCTAGC AAAAACACAC 
CAGGGAGCCG CTGCCATGGC AGGTGCTGGC GTCACTCAGA CTGTGTGTTT GCCTCTTCTT 
AGCGTGTACC AACTGAGTTC CAATGGGGCC AGTACGGCTC AGCCAGCTCT GTCGCTCCGC 
AAATCCCTGG ATGCCCCTTC CTGGCCTGGG GTCTACCGAC TTACTGTGTC ACTCATGGAG 
CGCCTGCTGA AGACCTTGAG GTACAACTTC CTGACCGAGG CGCTGGACTT TGTAGGAGTT 
CATCAGGAAC GGATTCTACA GTGTCTGGGC GCTGTGCGCA CCGTACCGAG CCTGGCCTGT 
TTGGAAGAGG CAGATCATAC GGTGGGCTTT CTGCTGCAGC TTTCCAACTT CACCAAAGAG 
TGGCATTTCC ACCTCCCGCA GCTGATTAAG GATGTTCAGG TGAATCTCTG TTACCTGTGC 
CAGGCCTGCA CCTCTCTCCT GCACAGCCGC AAGATGCTGC AGCACTACCT ACAGATAAAG 
AATGGAGAAA CCATGTCCTC CACTGCAACC CCTCGTGGGC AGCGCACTCC CCAGACGCCA 
TCTAAGCAGC CGACAGCAGA GAGTGAGGCG CTGGAGCTGA GGCAACTGCG TTCTGTTCAG 
CACAGCCTCC TGAAAATTTT GGGCAAAACT CTGGCCACCC TGCGAGCATT TACCCCTGAC 
CTCTGCCAAA TCCTCCAGGT TCAGCCTCTG GACCTGGCCC AATATAATCT CCTGTTTGCA 
CTGAGTTTCA CCACACCAGC TTTTGATGCA GACGTTACCC CTTCCTTTGG GACCCTTTTA 
GCTACCGTGA ATGTAACGCT GAGCATGCTG GGAGAGATGG ATAAAAAGAA AGATCACCCC 
CTCGGTCAAG TACTGGGAGA GACCAACAGC ACAGTAGACA ACAAGAACAT CAAGTCTTTG 
CTGCTCTTCA TTATGGAGAA CTGCTTCTAC CTGTTGATAT CACAAGCTGT GCGTTACCTT 
CGGGACCCTT CCGTTCACCC CCGGGACAAG CAGCGCATGA AGCAAGAACT AAGCTCGGAG 
CTGAGTACCC TGCTGTCCAG CCTGTCTCGT TACTTCCGGA GAGGTGGCCC CTCGTCTCCT 
GCCGGCGGCC TCATGCCCTC CCCTCAGCCT AAGGGTGCAT CGGCTGCAAA GGTGGTCCCT 
GAAGCCCAGG AGCCCCTCAT CCAGCTGGTA CAAGCCTTTG TGCGCCATGT ACAGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nup188 ( XM_012967947.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823401.1 Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X1, mRNA.

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CloneID	OXa31153
Clone ID Related Accession (Same CDS sequence)	XM_012967947.3 , XM_012967947.2
Accession Version	XM_012967947.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nucleoporin NUP188 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGGGTGTCT GTACCGGAGG ACCAGCCGAG AGTTATGGAC CATTCTGCTT 
GGACGCTCTT CCATGAAAGA TGGGCATCAG ATCAAAGCAG AGCTGGACAG ATATGGAGAC 
CGGCTCCTGC AGGGACTGGC ATACTACAGG CCCCCCAGCA CAAGCTCCAC AGGCAAAGTG 
AAAGCCAATA AGAATCTGTC CCCTGCCCTA CAGGAGCTTG GTTTGCGACT CAGCAAATTT 
CTGGGCCTGG ATGAAGAGCA GAGCGTGGAG CTGTTACAGA CTTACCTGCT GTATGATTAC 
AGAGGGACAC AGGAATCTGT AAAGGGTGTG CCACAAGATG AGAGGCAGAG TCAAGCACTG 
ATGCTGAAGA TGGTAGATTA CTACTATGAA GAGAGAATTT CCCTGCTGCG CTGTGTCCTG 
TACATTCTGA ATTATTTCCA GGATGACAAG CATCCCTATA GCGCAGAATT CTCCAAATGT 
GTGGAAATGA TGGAGCAGAA GGAATTATTC GGCAAGTACC TGAAGCAGTT TGAAAGCCTC 
TGCAGGGAGG AGGCCCCAAC ATGGGAAACA CATGGAAACT TCATGACGGA GCGCCAGGTG 
TCCCGGTGGT TTGTGCAGCG ATTGCGGGAG CAGGCCATGC TCTTGGAGAT CATATTCCTA 
TATTTTGCCT GTTTTGCTGC TTCCCCATCC GACCTGCTAG CACTGACCAA ACTGTTCAAG 
GAGCAAGGCT TCGGGTGCAG GCTGCAAAAC CGCCACCTTG TAGAACCATC AATGGACCCT 
CTGGTGGAGA GAATCGGGTA CTTCTCTGTT TTAATCTTTT TGGAAGCGCT AGACATTGAA 
ACTCTTATGA CCTGCTCTCT CAGTGATAAA ACAGAGCAGC ATCCATTTTC TTCTGAGGAG 
CAGGTCTGCA AGGAGATGGA CAGTGTTTTG GTTACGCTGG GAGACGCCCC ACACCATGGA 
CCTGTGCTGC TGGCCTGGGC CCTGCTGCGT TTTGCTCTGA ATGCTGACAA GGTTACCCCC 
ACTGTCCGTA AAATGGGCAG CACCGCCATC CAGCTCCATA TCTTTCAGTA CTTAACCCGA 
ATGCTTCAAT CTCTTAGAAG TGGGGAGAAT AATTGTACGA CCAGCACGGC TTGTTTGTGT 
GTCTACACCT TCCTGACTTT TGTGCTCACA AACCTAGAGG AACAAGTCTT ACAGAGCCAG 
CAGGATCTTG TAGACACAGC TTTCCAGGTG TTTGCTGCTC CGAATCTGCC TGATCTGTTC 
TGGAACATGG AACCCACAGC AGGCCTGGGG ATCCTTCTGG ACAGCGTGGT TGGGATGTTC 
CCCTTTCACC TCTCGCCTCT CCTGAAGCTT TTCACTGCGC TTGTCTCCAA GTCTTCTGCA 
AAGAAGGTGT ACAGTTTTCT AGACCGGATG TCCTCTTACA CAGAGCACTA CCGCCACAAG 
CCTCACGATA TTCTCTCGCA CGAGGATGAG ACACTATGGA AGAGACAGAC CCCTAAAGTG 
CTGTATGCAC TAGGACTGGG ACAAACAAAC TTGCGCATCC CTCAAGGGAC CATAGGGCAG 
GTGATGGCCG ATGAACATGG CTTCCTGGTC CGCTGGGAGT ATTCCTACAG CTGCTGGACT 
CTGTTTACCT GTGAGATCGA GATGCTGCTG CATGTTGTGT CCACAGCTGA TGTGATCCAC 
CAGTGCCAAA GGGTGAAACC CATCATTGAC CTAGTTCATA AGGTTATCAG CACAGACTTG 
TCCATAGCTG ACTGTCTTCT GCCCATCACA TCCCGGATTT ACATGCTCCT ACAGAGGTTA 
ACGACAGTAA TGAACCCGCC GATGGATTTC CTCTCTTCTT GTGTTGACTG CCTCGCTGCT 
TTAGCCACAC GTATGCCTGC AAAGGTTTGG ATAGACCTAC GACACACAGG GTTCTTGCCC 
TTTGCAGCAA ATCCTGTCTC TGGGCACATC ATTAGCACTG AAGGGATGAA TGCTGGCGGT 
TACGGCTCAC TTTTCGGCAT TGAGCAGTCA CAAGGGGAAT ACAGCATCAC GCTCTCTTTT 
CTCCGTCTGG TCACCACCTT AGTGAAGGGG CAGTTGGGAA GCACCCAAAG CCAGGGCCTT 
GTGCCCTGTA TCCTGTTTGT GCTGCGTGAA ATGCTTCCTA ACTACCACAG GTGGCGCTAT 
AATAGTCACG GCGTGAGAGA ACAACTTGGC TTCCAGATCC TGAGTTTGAT TCATGCAATC 
CTCAACTTAT CCCCTGAGGA AGAGGAGAGC ACCAGCACCC CAAACTTGCA GTCCCTGTGC 
ATCTTCAGCC TGACAAACAC TGAGGCAGGA CAGGCGGTCA TTAACATCAT GGGGGTCGGA 
GTGGACACGC TCAATGCGGT CATGCTGACT CAGGCTGGGA GCAGTGGCAC AGAGGGCCAA 
GGGCAGATGC TCATGCAAAC CATCAAACTG GCTTTTTCCA TCACCAATAA TGTGATCAGA 
CTGAAGCCCC TTTCTAGTGC CATCTCGCCA CTGGAGCATG CACTCACTCA GCACGGAGCT 
CATGGGAACA ATCTAATTGC TGTCCTGGCC AAATATATTT ATCACAAATA TGATCCGTCG 
CTGCCCCGGC TGGCCATCCA GCTGCTGAAA CGCCTGGCCA TGGTGGCACC TATGTCAGTG 
TACGCCTGTT TGGGCAGTGA TGCAGCCGCC ATTAGGGATG CCTTCCTGAG CCGCCTGCGG 
GATAACATTG AGGACATGCA GATCAAGATT ATGATATTGG AATTCCTTAC TGTTGCCGTG 
GAGACGCAGC CTGGACTCAT TGAGCTTTTC CTCAATTTAG AGGTGAAGGA CACAAACGAG 
GGCTCAAAGG AGTACAGCCT CGGGGAGTGG AGCTGTTTGC AGGTTGTGCT GAAGCTTATT 
GATTCCCAAG ATCCCGAGAG CTCCTGGGGA GCCCCTCTGC TCCACCGCTC GGCCATCGCT 
TTCCTCCACG CCCTTTGGCA GGATCGGCGG GACAGCGCCA TGACTGTGCT CAGAACCAAG 
CCCAACTTCT GGGAGAACCT GACAAGCCCC CTGTTTGGGA CACTGGCGTT TCCATCGGAA 
TCCTCAGAGC TGAGCATCCT GGAGACCTGC GCTTTCATAA TGAAAATTAT TTGCCTCGAG 
ATTTACTATG CAGTGAGGGG TTCTCTTGGT GACTCCCTGA AAAAGATCCT AAAGAAATTC 
TCTGAGGAAG AGCGATTCAC TTACTGGTCT AACTACGTAC ATTCCTTGGT GTGCCAGGTG 
GCTGAGAGTG AGGGGGCCTG TAACTCGCTC ACTGAGTATC AGCAGCTGCT GTCAGCTTGG 
CGGATGTTTC TCATGGTCGC CACGCACAAT GCAGATGTAA TGCACCTGAC CAATCCTGAG 
GTCCGACAGA AGCTATTTAA GGATGTCTTG GGCGGCACTC AGGCTCTGCT TCTTGTTCCC 
AGATCCATGA CTTGCCTGCA CCTCGGCTCC ATGCTTTGCA CTGTGATGAT CATCTTACTC 
AGGAGGTGGA AAAGTGACCT AGCAGCGCCT GAGGACATTC TCAGCTCTCT GACACAGATT 
CTGGAGGGGG TCCTGCAGAA AGATCAGCAG CTGGTGGAGA AGACCAAAGC CCGAGTCTTT 
GCCGCCCTCA TCTCTGCGCT AGAGATAAAG CCAATGAAAG CCAGTGAGAT CCCCCAGTAC 
CCCCAATTGG TGCTCAATGT GTGCGAGACC CTGCAGGAAG AAGTGGTGTT TCTTGTGGAT 
CACACACGCC AAGAGGTCCC CGCTAACGAC GCATCCGAGG ACAAGGACAG CATGGAGACC 
GAGGACACAG GCCGGATCCG GCAGAAGGAC CAGCGTGATG GAGTGTGCGT GCTGGGGCTG 
CATTTGGCCA AGGGACTGTG TGAGGCGGAC GAGGAGGGTG ACCAGTGGCA GCAGGTACTC 
AGGAAGCTTC CAGTTCTGCC CATGCTCTTC AGTGCCCTGG AGGTCAGCCT GCGCATCAAG 
CAGAATCTCC ACTTCTGTGA AGCCATTCTC CACTTCCTGT TCACTCTAGC AAAAACACAC 
CAGGGAGCCG CTGCCATGGC AGGTGCTGGC GTCACTCAGA CTGTGTGTTT GCCTCTTCTT 
AGCGTGTACC AACTGAGTTC CAATGGGGCC AGTACGGCTC AGCCAGCTCT GTCGCTCCGC 
AAATCCCTGG ATGCCCCTTC CTGGCCTGGG GTCTACCGAC TTACTGTGTC ACTCATGGAG 
CGCCTGCTGA AGACCTTGAG GTACAACTTC CTGACCGAGG CGCTGGACTT TGTAGGAGTT 
CATCAGGAAC GGATTCTACA GTGTCTGGGC GCTGTGCGCA CCGTACCGAG CCTGGCCTGT 
TTGGAAGAGG CAGATCATAC GGTGGGCTTT CTGCTGCAGC TTTCCAACTT CACCAAAGAG 
TGGCATTTCC ACCTCCCGCA GCTGATTAAG GATGTTCAGA TAAAGAATGG AGAAACCATG 
TCCTCCACTG CAACCCCTCG TGGGCAGCGC ACTCCCCAGA CGCCATCTAA GCAGCCGACA 
GCAGAGAGTG AGGCGCTGGA GCTGAGGCAA CTGCGTTCTG TTCAGCACAG CCTCCTGAAA 
ATTTTGGGCA AAACTCTGGC CACCCTGCGA GCATTTACCC CTGACCTCTG CCAAATCCTC 
CAGGTTCAGC CTCTGGACCT GGCCCAATAT AATCTCCTGT TTGCACTGAG TTTCACCACA 
CCAGCTTTTG ATGCAGACGT TACCCCTTCC TTTGGGACCC TTTTAGCTAC CGTGAATGTA 
ACGCTGAGCA TGCTGGGAGA GATGGATAAA AAGAAAGATC ACCCCCTCGG TCAAGTACTG 
GGAGAGACCA ACAGCACAGT AGACAACAAG AACATCAAGT CTTTGCTGCT CTTCATTATG 
GAGAACTGCT TCTACCTGTT GATATCACAA GCTGTGCGTT ACCTTCGGGA CCCTTCCGTT 
CACCCCCGGG ACAAGCAGCG CATGAAGCAA GAACTAAGCT CGGAGCTGAG TACCCTGCTG 
TCCAGCCTGT CTCGTTACTT CCGGAGAGGT GGCCCCTCGT CTCCTGCCGG CGGCCTCATG 
CCCTCCCCTC AGCCTAAGGG TGCATCGGCT GCAAAGGTGG TCCCTGAAGC CCAGGAGCCC 
CTCATCCAGC TGGTACAAGC CTTTGTGCGC CATGTACAGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823401.1
CDS	70..5214
Translation	MAAAGCLYRRTSRELWTILLGRSSMKDGHQIKAELDRYGDRLLQGLAYYRPPSTSSTGKVKANKNLSPALQELGLRLSKFLGLDEEQSVELLQTYLLYDYRGTQESVKGVPQDERQSQALMLKMVDYYYEERISLLRCVLYILNYFQDDKHPYSAEFSKCVEMMEQKELFGKYLKQFESLCREEAPTWETHGNFMTERQVSRWFVQRLREQAMLLEIIFLYFACFAASPSDLLALTKLFKEQGFGCRLQNRHLVEPSMDPLVERIGYFSVLIFLEALDIETLMTCSLSDKTEQHPFSSEEQVCKEMDSVLVTLGDAPHHGPVLLAWALLRFALNADKVTPTVRKMGSTAIQLHIFQYLTRMLQSLRSGENNCTTSTACLCVYTFLTFVLTNLEEQVLQSQQDLVDTAFQVFAAPNLPDLFWNMEPTAGLGILLDSVVGMFPFHLSPLLKLFTALVSKSSAKKVYSFLDRMSSYTEHYRHKPHDILSHEDETLWKRQTPKVLYALGLGQTNLRIPQGTIGQVMADEHGFLVRWEYSYSCWTLFTCEIEMLLHVVSTADVIHQCQRVKPIIDLVHKVISTDLSIADCLLPITSRIYMLLQRLTTVMNPPMDFLSSCVDCLAALATRMPAKVWIDLRHTGFLPFAANPVSGHIISTEGMNAGGYGSLFGIEQSQGEYSITLSFLRLVTTLVKGQLGSTQSQGLVPCILFVLREMLPNYHRWRYNSHGVREQLGFQILSLIHAILNLSPEEEESTSTPNLQSLCIFSLTNTEAGQAVINIMGVGVDTLNAVMLTQAGSSGTEGQGQMLMQTIKLAFSITNNVIRLKPLSSAISPLEHALTQHGAHGNNLIAVLAKYIYHKYDPSLPRLAIQLLKRLAMVAPMSVYACLGSDAAAIRDAFLSRLRDNIEDMQIKIMILEFLTVAVETQPGLIELFLNLEVKDTNEGSKEYSLGEWSCLQVVLKLIDSQDPESSWGAPLLHRSAIAFLHALWQDRRDSAMTVLRTKPNFWENLTSPLFGTLAFPSESSELSILETCAFIMKIICLEIYYAVRGSLGDSLKKILKKFSEEERFTYWSNYVHSLVCQVAESEGACNSLTEYQQLLSAWRMFLMVATHNADVMHLTNPEVRQKLFKDVLGGTQALLLVPRSMTCLHLGSMLCTVMIILLRRWKSDLAAPEDILSSLTQILEGVLQKDQQLVEKTKARVFAALISALEIKPMKASEIPQYPQLVLNVCETLQEEVVFLVDHTRQEVPANDASEDKDSMETEDTGRIRQKDQRDGVCVLGLHLAKGLCEADEEGDQWQQVLRKLPVLPMLFSALEVSLRIKQNLHFCEAILHFLFTLAKTHQGAAAMAGAGVTQTVCLPLLSVYQLSSNGASTAQPALSLRKSLDAPSWPGVYRLTVSLMERLLKTLRYNFLTEALDFVGVHQERILQCLGAVRTVPSLACLEEADHTVGFLLQLSNFTKEWHFHLPQLIKDVQIKNGETMSSTATPRGQRTPQTPSKQPTAESEALELRQLRSVQHSLLKILGKTLATLRAFTPDLCQILQVQPLDLAQYNLLFALSFTTPAFDADVTPSFGTLLATVNVTLSMLGEMDKKKDHPLGQVLGETNSTVDNKNIKSLLLFIMENCFYLLISQAVRYLRDPSVHPRDKQRMKQELSSELSTLLSSLSRYFRRGGPSSPAGGLMPSPQPKGASAAKVVPEAQEPLIQLVQAFVRHVQR

Target ORF information:

RefSeq Version	XM_012967947.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nucleoporin 188kDa (nup188), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967947.2

ORF Insert Sequence:

ATGGCGGCGG CCGGGTGTCT GTACCGGAGG ACCAGCCGAG AGTTATGGAC CATTCTGCTT 
GGACGCTCTT CCATGAAAGA TGGGCATCAG ATCAAAGCAG AGCTGGACAG ATATGGAGAC 
CGGCTCCTGC AGGGACTGGC ATACTACAGG CCCCCCAGCA CAAGCTCCAC AGGCAAAGTG 
AAAGCCAATA AGAATCTGTC CCCTGCCCTA CAGGAGCTTG GTTTGCGACT CAGCAAATTT 
CTGGGCCTGG ATGAAGAGCA GAGCGTGGAG CTGTTACAGA CTTACCTGCT GTATGATTAC 
AGAGGGACAC AGGAATCTGT AAAGGGTGTG CCACAAGATG AGAGGCAGAG TCAAGCACTG 
ATGCTGAAGA TGGTAGATTA CTACTATGAA GAGAGAATTT CCCTGCTGCG CTGTGTCCTG 
TACATTCTGA ATTATTTCCA GGATGACAAG CATCCCTATA GCGCAGAATT CTCCAAATGT 
GTGGAAATGA TGGAGCAGAA GGAATTATTC GGCAAGTACC TGAAGCAGTT TGAAAGCCTC 
TGCAGGGAGG AGGCCCCAAC ATGGGAAACA CATGGAAACT TCATGACGGA GCGCCAGGTG 
TCCCGGTGGT TTGTGCAGCG ATTGCGGGAG CAGGCCATGC TCTTGGAGAT CATATTCCTA 
TATTTTGCCT GTTTTGCTGC TTCCCCATCC GACCTGCTAG CACTGACCAA ACTGTTCAAG 
GAGCAAGGCT TCGGGTGCAG GCTGCAAAAC CGCCACCTTG TAGAACCATC AATGGACCCT 
CTGGTGGAGA GAATCGGGTA CTTCTCTGTT TTAATCTTTT TGGAAGCGCT AGACATTGAA 
ACTCTTATGA CCTGCTCTCT CAGTGATAAA ACAGAGCAGC ATCCATTTTC TTCTGAGGAG 
CAGGTCTGCA AGGAGATGGA CAGTGTTTTG GTTACGCTGG GAGACGCCCC ACACCATGGA 
CCTGTGCTGC TGGCCTGGGC CCTGCTGCGT TTTGCTCTGA ATGCTGACAA GGTTACCCCC 
ACTGTCCGTA AAATGGGCAG CACCGCCATC CAGCTCCATA TCTTTCAGTA CTTAACCCGA 
ATGCTTCAAT CTCTTAGAAG TGGGGAGAAT AATTGTACGA CCAGCACGGC TTGTTTGTGT 
GTCTACACCT TCCTGACTTT TGTGCTCACA AACCTAGAGG AACAAGTCTT ACAGAGCCAG 
CAGGATCTTG TAGACACAGC TTTCCAGGTG TTTGCTGCTC CGAATCTGCC TGATCTGTTC 
TGGAACATGG AACCCACAGC AGGCCTGGGG ATCCTTCTGG ACAGCGTGGT TGGGATGTTC 
CCCTTTCACC TCTCGCCTCT CCTGAAGCTT TTCACTGCGC TTGTCTCCAA GTCTTCTGCA 
AAGAAGGTGT ACAGTTTTCT AGACCGGATG TCCTCTTACA CAGAGCACTA CCGCCACAAG 
CCTCACGATA TTCTCTCGCA CGAGGATGAG ACACTATGGA AGAGACAGAC CCCTAAAGTG 
CTGTATGCAC TAGGACTGGG ACAAACAAAC TTGCGCATCC CTCAAGGGAC CATAGGGCAG 
GTGATGGCCG ATGAACATGG CTTCCTGGTC CGCTGGGAGT ATTCCTACAG CTGCTGGACT 
CTGTTTACCT GTGAGATCGA GATGCTGCTG CATGTTGTGT CCACAGCTGA TGTGATCCAC 
CAGTGCCAAA GGGTGAAACC CATCATTGAC CTAGTTCATA AGGTTATCAG CACAGACTTG 
TCCATAGCTG ACTGTCTTCT GCCCATCACA TCCCGGATTT ACATGCTCCT ACAGAGGTTA 
ACGACAGTAA TGAACCCGCC GATGGATTTC CTCTCTTCTT GTGTTGACTG CCTCGCTGCT 
TTAGCCACAC GTATGCCTGC AAAGGTTTGG ATAGACCTAC GACACACAGG GTTCTTGCCC 
TTTGCAGCAA ATCCTGTCTC TGGGCACATC ATTAGCACTG AAGGGATGAA TGCTGGCGGT 
TACGGCTCAC TTTTCGGCAT TGAGCAGTCA CAAGGGGAAT ACAGCATCAC GCTCTCTTTT 
CTCCGTCTGG TCACCACCTT AGTGAAGGGG CAGTTGGGAA GCACCCAAAG CCAGGGCCTT 
GTGCCCTGTA TCCTGTTTGT GCTGCGTGAA ATGCTTCCTA ACTACCACAG GTGGCGCTAT 
AATAGTCACG GCGTGAGAGA ACAACTTGGC TTCCAGATCC TGAGTTTGAT TCATGCAATC 
CTCAACTTAT CCCCTGAGGA AGAGGAGAGC ACCAGCACCC CAAACTTGCA GTCCCTGTGC 
ATCTTCAGCC TGACAAACAC TGAGGCAGGA CAGGCGGTCA TTAACATCAT GGGGGTCGGA 
GTGGACACGC TCAATGCGGT CATGCTGACT CAGGCTGGGA GCAGTGGCAC AGAGGGCCAA 
GGGCAGATGC TCATGCAAAC CATCAAACTG GCTTTTTCCA TCACCAATAA TGTGATCAGA 
CTGAAGCCCC TTTCTAGTGC CATCTCGCCA CTGGAGCATG CACTCACTCA GCACGGAGCT 
CATGGGAACA ATCTAATTGC TGTCCTGGCC AAATATATTT ATCACAAATA TGATCCGTCG 
CTGCCCCGGC TGGCCATCCA GCTGCTGAAA CGCCTGGCCA TGGTGGCACC TATGTCAGTG 
TACGCCTGTT TGGGCAGTGA TGCAGCCGCC ATTAGGGATG CCTTCCTGAG CCGCCTGCGG 
GATAACATTG AGGACATGCA GATCAAGATT ATGATATTGG AATTCCTTAC TGTTGCCGTG 
GAGACGCAGC CTGGACTCAT TGAGCTTTTC CTCAATTTAG AGGTGAAGGA CACAAACGAG 
GGCTCAAAGG AGTACAGCCT CGGGGAGTGG AGCTGTTTGC AGGTTGTGCT GAAGCTTATT 
GATTCCCAAG ATCCCGAGAG CTCCTGGGGA GCCCCTCTGC TCCACCGCTC GGCCATCGCT 
TTCCTCCACG CCCTTTGGCA GGATCGGCGG GACAGCGCCA TGACTGTGCT CAGAACCAAG 
CCCAACTTCT GGGAGAACCT GACAAGCCCC CTGTTTGGGA CACTGGCGTT TCCATCGGAA 
TCCTCAGAGC TGAGCATCCT GGAGACCTGC GCTTTCATAA TGAAAATTAT TTGCCTCGAG 
ATTTACTATG CAGTGAGGGG TTCTCTTGGT GACTCCCTGA AAAAGATCCT AAAGAAATTC 
TCTGAGGAAG AGCGATTCAC TTACTGGTCT AACTACGTAC ATTCCTTGGT GTGCCAGGTG 
GCTGAGAGTG AGGGGGCCTG TAACTCGCTC ACTGAGTATC AGCAGCTGCT GTCAGCTTGG 
CGGATGTTTC TCATGGTCGC CACGCACAAT GCAGATGTAA TGCACCTGAC CAATCCTGAG 
GTCCGACAGA AGCTATTTAA GGATGTCTTG GGCGGCACTC AGGCTCTGCT TCTTGTTCCC 
AGATCCATGA CTTGCCTGCA CCTCGGCTCC ATGCTTTGCA CTGTGATGAT CATCTTACTC 
AGGAGGTGGA AAAGTGACCT AGCAGCGCCT GAGGACATTC TCAGCTCTCT GACACAGATT 
CTGGAGGGGG TCCTGCAGAA AGATCAGCAG CTGGTGGAGA AGACCAAAGC CCGAGTCTTT 
GCCGCCCTCA TCTCTGCGCT AGAGATAAAG CCAATGAAAG CCAGTGAGAT CCCCCAGTAC 
CCCCAATTGG TGCTCAATGT GTGCGAGACC CTGCAGGAAG AAGTGGTGTT TCTTGTGGAT 
CACACACGCC AAGAGGTCCC CGCTAACGAC GCATCCGAGG ACAAGGACAG CATGGAGACC 
GAGGACACAG GCCGGATCCG GCAGAAGGAC CAGCGTGATG GAGTGTGCGT GCTGGGGCTG 
CATTTGGCCA AGGGACTGTG TGAGGCGGAC GAGGAGGGTG ACCAGTGGCA GCAGGTACTC 
AGGAAGCTTC CAGTTCTGCC CATGCTCTTC AGTGCCCTGG AGGTCAGCCT GCGCATCAAG 
CAGAATCTCC ACTTCTGTGA AGCCATTCTC CACTTCCTGT TCACTCTAGC AAAAACACAC 
CAGGGAGCCG CTGCCATGGC AGGTGCTGGC GTCACTCAGA CTGTGTGTTT GCCTCTTCTT 
AGCGTGTACC AACTGAGTTC CAATGGGGCC AGTACGGCTC AGCCAGCTCT GTCGCTCCGC 
AAATCCCTGG ATGCCCCTTC CTGGCCTGGG GTCTACCGAC TTACTGTGTC ACTCATGGAG 
CGCCTGCTGA AGACCTTGAG GTACAACTTC CTGACCGAGG CGCTGGACTT TGTAGGAGTT 
CATCAGGAAC GGATTCTACA GTGTCTGGGC GCTGTGCGCA CCGTACCGAG CCTGGCCTGT 
TTGGAAGAGG CAGATCATAC GGTGGGCTTT CTGCTGCAGC TTTCCAACTT CACCAAAGAG 
TGGCATTTCC ACCTCCCGCA GCTGATTAAG GATGTTCAGA TAAAGAATGG AGAAACCATG 
TCCTCCACTG CAACCCCTCG TGGGCAGCGC ACTCCCCAGA CGCCATCTAA GCAGCCGACA 
GCAGAGAGTG AGGCGCTGGA GCTGAGGCAA CTGCGTTCTG TTCAGCACAG CCTCCTGAAA 
ATTTTGGGCA AAACTCTGGC CACCCTGCGA GCATTTACCC CTGACCTCTG CCAAATCCTC 
CAGGTTCAGC CTCTGGACCT GGCCCAATAT AATCTCCTGT TTGCACTGAG TTTCACCACA 
CCAGCTTTTG ATGCAGACGT TACCCCTTCC TTTGGGACCC TTTTAGCTAC CGTGAATGTA 
ACGCTGAGCA TGCTGGGAGA GATGGATAAA AAGAAAGATC ACCCCCTCGG TCAAGTACTG 
GGAGAGACCA ACAGCACAGT AGACAACAAG AACATCAAGT CTTTGCTGCT CTTCATTATG 
GAGAACTGCT TCTACCTGTT GATATCACAA GCTGTGCGTT ACCTTCGGGA CCCTTCCGTT 
CACCCCCGGG ACAAGCAGCG CATGAAGCAA GAACTAAGCT CGGAGCTGAG TACCCTGCTG 
TCCAGCCTGT CTCGTTACTT CCGGAGAGGT GGCCCCTCGT CTCCTGCCGG CGGCCTCATG 
CCCTCCCCTC AGCCTAAGGG TGCATCGGCT GCAAAGGTGG TCCCTGAAGC CCAGGAGCCC 
CTCATCCAGC TGGTACAAGC CTTTGTGCGC CATGTACAGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rare copy number variations in congenital heart disease patients identify unique genes in left-right patterning.
Proceedings of the National Academy of Sciences of the United States of America108(7)2915-20(2011 Feb)
Fakhro KA,Choi M,Ware SM,Belmont JW,Towbin JA,Lifton RP,Khokha MK,Brueckner M

The genome of the Western clawed frog Xenopus tropicalis.
Science (New York, N.Y.)328(5978)633-6(2010 Apr)
Hellsten U,Harland RM,Gilchrist MJ,Hendrix D,Jurka J,Kapitonov V,Ovcharenko I,Putnam NH,Shu S,Taher L,Blitz IL,Blumberg B,Dichmann DS,Dubchak I,Amaya E,Detter JC,Fletcher R,Gerhard DS,Goodstein D,Graves T,Grigoriev IV,Grimwood J,Kawashima T,Lindquist E,Lucas SM,Mead PE,Mitros T,Ogino H,Ohta Y,Poliakov AV,Pollet N,Robert J,Salamov A,Sater AK,Schmutz J,Terry A,Vize PD,Warren WC,Wells D,Wills A,Wilson RK,Zimmerman LB,Zorn AM,Grainger R,Grammer T,Khokha MK,Richardson PM,Rokhsar DS

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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nup188 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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