a2ml1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following a2ml1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the a2ml1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa61177 XM_031904778.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61178 XM_031904779.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61179 XM_031904780.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61180 XM_012954234.3
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61181 XM_031904781.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61182 XM_031904782.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61183 XM_031904783.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61184 XM_031904784.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa61184 XM_031904785.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa22824 NM_001113027.1
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa31002 XM_012954234.2
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa31003 XM_012954235.2
Latest version!
Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa61177
    Clone ID Related Accession (Same CDS sequence) XM_031904778.1
    Accession Version XM_031904778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAG TTAATTTCTC CTTCATGGTG GAACAAGAGG TTCTGGTGAA GAATCTCAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGTGG AACATGCAGT TACCGAGTAC
    AACTCCCCCT GCAGCTCGGA TGAAGACACG GGCAACATAA TACAAGGAGT AGAAGCGCCT
    CGCTATATCA GAGATCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760638.1
    CDS186..4646
    Translation

    Target ORF information:

    RefSeq Version XM_031904778.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAG TTAATTTCTC CTTCATGGTG GAACAAGAGG TTCTGGTGAA GAATCTCAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGTGG AACATGCAGT TACCGAGTAC
    AACTCCCCCT GCAGCTCGGA TGAAGACACG GGCAACATAA TACAAGGAGT AGAAGCGCCT
    CGCTATATCA GAGATCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61178
    Clone ID Related Accession (Same CDS sequence) XM_031904779.1
    Accession Version XM_031904779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCTCTACA GGCTGTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTTTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTATATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC TGATAAGAAA AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC ATAGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAA TTAATTTCTC CTTCATGGTG GAACAAGAGA TTCTGGTGAA GAATCTTAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGCGG AACATGCAGT TACCGAGTAC
    AACTCCCCCT GCAGCTCCGA TGAAGATACG GGCAACATAA TACAAGGAGT AGATGCGCCT
    CACTATATCA GAGACCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760639.1
    CDS184..4644
    Translation

    Target ORF information:

    RefSeq Version XM_031904779.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCTCTACA GGCTGTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTTTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTATATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC TGATAAGAAA AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC ATAGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAA TTAATTTCTC CTTCATGGTG GAACAAGAGA TTCTGGTGAA GAATCTTAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGCGG AACATGCAGT TACCGAGTAC
    AACTCCCCCT GCAGCTCCGA TGAAGATACG GGCAACATAA TACAAGGAGT AGATGCGCCT
    CACTATATCA GAGACCCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61179
    Clone ID Related Accession (Same CDS sequence) XM_031904780.1
    Accession Version XM_031904780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAG TTAATTTCTC CTTCATGGTG GAACAAGAGG TTCTGGTGAA GAATCTCAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGGGC ACCATATGCC ATTGGCCTAT
    GGGAGCAGCC CTTCCCTGTC TAGAAGACTT TTCCTAAATA AAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760640.1
    CDS186..4613
    Translation

    Target ORF information:

    RefSeq Version XM_031904780.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAG TTAATTTCTC CTTCATGGTG GAACAAGAGG TTCTGGTGAA GAATCTCAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGGGC ACCATATGCC ATTGGCCTAT
    GGGAGCAGCC CTTCCCTGTC TAGAAGACTT TTCCTAAATA AAGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61180
    Clone ID Related Accession (Same CDS sequence) XM_012954234.3
    Accession Version XM_012954234.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012954234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACAGTTG TAGCTCTACA GGCTCTTGCT
    AAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCAG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCAAA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAG AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC CGTGAAGAGC ACAATAAAGC AGCTAGTGAG CAGCAATACG
    ATAAAGCGGA GTGAGATCCA AACTGATATG GTGACTTTGT ACCTGAATGA GTTAGGACAT
    GATCCAGTTC ACTTCTCGAT TATGGTGGAA CAAGACATTG AGGTGAAGAA CCTAAAGCCG
    GCCACGGTGA AGGTCTATGA TTATTATGAG ACAGCGGAAC ATGCAGTTAC CGAGTACAAC
    TCCCCCTGCA GCTCGGATAA AGAAGCAGAA AACACAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809688.2
    CDS159..4580
    Translation

    Target ORF information:

    RefSeq Version XM_012954234.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954234.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACAGTTG TAGCTCTACA GGCTCTTGCT
    AAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCAG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCAAA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAG AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC CGTGAAGAGC ACAATAAAGC AGCTAGTGAG CAGCAATACG
    ATAAAGCGGA GTGAGATCCA AACTGATATG GTGACTTTGT ACCTGAATGA GTTAGGACAT
    GATCCAGTTC ACTTCTCGAT TATGGTGGAA CAAGACATTG AGGTGAAGAA CCTAAAGCCG
    GCCACGGTGA AGGTCTATGA TTATTATGAG ACAGCGGAAC ATGCAGTTAC CGAGTACAAC
    TCCCCCTGCA GCTCGGATAA AGAAGCAGAA AACACAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61181
    Clone ID Related Accession (Same CDS sequence) XM_031904781.1
    Accession Version XM_031904781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAG TTAATTTCTC CTTCATGGTG GAACAAGAGG TTCTGGTGAA GAATCTCAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGATG AAGACACGGG CAACATAATA
    CAAGGAGTAG AAGCGCCTCG CTATATCAGA GATCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760641.1
    CDS186..4604
    Translation

    Target ORF information:

    RefSeq Version XM_031904781.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGTTAGGA
    CGTCATCCAG TTAATTTCTC CTTCATGGTG GAACAAGAGG TTCTGGTGAA GAATCTCAGA
    CCAGCCATAG TGAAAGTCTA TGATTATTAT GAGCCAGATG AAGACACGGG CAACATAATA
    CAAGGAGTAG AAGCGCCTCG CTATATCAGA GATCCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61182
    Clone ID Related Accession (Same CDS sequence) XM_031904782.1
    Accession Version XM_031904782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCTGCC ATAGCTCCGA ACAAGCCTGT GTGCACCTCA GTGGGGCGGA GGGAGACAGC 
    CGGATACAGG TTACCTTGAA CATGGCCGAA CGGAACTCAA CGGTGATAGA GAGGAACCTC
    CGGCAGAAAT CCCTCTTCAG CTGTGTTACA TTCCAGGTGC CTCCGCCCTC TGAAGGGAAT
    GAAGAAGTGG CCACCATGGA AATTCTCATT GAGAGCGCAG GGGAGACCAT AACCAACAGC
    AGCAAAGTTC TAGTGAAGAG GAGAAGAACC AGTACTTTCA TTCAGACGGA CAAAGCTGTA
    TATAAACCAG GGCAAACAGT ACGATTCCGA GTGGTTTCTC TTAAAGAAAA TCTACAACCT
    GGAGAAAATC AGATTTCAGT CATCCAATTA CAGGATCCAA ACAAGAACCG TATTGCCCAG
    TGGCTGGATG TGACCCTCAC ACAGGGAATC GCTGAGCTCT CACTGCCGCT GTCCCCGGAA
    CCTTCGCTTG GAGAATATTC CATTCACGTG GGGGACACTG TGCACTACTT TAGCGTGGAA
    GAATACGTCC TTCCCAAGTT TGAGGTCAGT TTCCAGTTCC CCAAGGTGGT GTTCTTTACC
    AGCGAACAAT TCCAGCTGAA AACATGTGGC AGATACACCT ATGGCAAACC TGTGCAAGGA
    ACCTATAAGG CTACGGTGTG CCGTAAGGTC ATAAGATACT GGTGGGGACA CAATGATGAC
    CGCTCAGACC TGTGCGTCGA TTTCAGCGGG AAGTTGGACC AGTCTGGCTG TTCCACATTG
    ACAGCGACGT CTGAAAACTT CAATCTTACC TACAACACTC ATGAAATGGG CCTAGAGGGG
    ACGTCTACTA TTACAGAGGG AGGGACAGGA GTGGAATATT CTGGAAGCGG TAAAACCATC
    ATTTCGTCTG TAGTCACGAG TTGCAGTTTC CTGGATGCTG ACAGTAACTA CAAGCCGGGG
    ATCCCATACA GCGGCACAGT AAAGGTGGTA GATGCCAATG ATGACCCCAT CCCCAACCAG
    ATAGTCTATC TAACCAGCCA TTCTAATGAA GTAAACAAGA CATTGGTGTC GGATGAGAAT
    GGACTCGCCT CCTTCCAGCT GGAGAACACG GCAGAGTGGA AGGGACAGCT GAGCCTTAGG
    GCCAGTACAA CTACACAGGA CGCTGGATTT GATTTCCTTC AGGGCCGTCT CATTACCCCT
    AATGATGTGA GTGCATCGCT CATGCTGAAA CCCTTCCACT CCCCCAGCAA GAGTTTCCTG
    AAACTGCACT CCCAGCAGGG GGCGCTCCCA TGTGAGGGGC AACGAGATGT CCGAGTGGAA
    TATATTATCC CAAACTCTGC CCTGGGGATT GAGGTCAAGC ACATGGAGCT GCACTACCTG
    GTTGCTTCTA AAGGGTCAGT AAAGGACTCG GGATCAGTGG AAGTGCCCAT TACTAGTGAA
    GGAGGAGGAT CTTCAGAGCT GAGGGGTGAG GTCTCCATCA AACTCACACT CAGTGCCGAC
    GTCTCTCCGA CTCTCCGCGC TCTCGCCTTC ATTTCCCTCC CTAATGGGGA ATTAGTGGCC
    GATTCTGCAA CATTCAAACT GCAAAGGTGC TTCAAGAACA AAGTATCAGT TGGCTTCTCC
    CCAGATGAAG TTCTCCCAGG AGCTGATGTT TCCCTACAAG TCCAGGCAGC CGCCGGGTCC
    CTGTGTGGCC TGAGAGTGGT GGATCAGAGT GTGGTGTTAA TGAAACCTGA CAAAGAACTG
    ACGGCCGATC AGATTCACAA TCTCTTTCCT TTCCCTGATT TCGGAGGGTA CGATGCTCGG
    GTCCAAGATA GTGATGATCA TTGTCCATTT TATCCCTGGT TTCATCGACC AATGTGGAAG
    AGAAGTATTG TTCTACCACC GTGGCATATG CCTCATGAAG ATGAGGTTGA TGTATACAGC
    TTATTTAAGG ACATGCGCCT TAAGATCGTC ACTAGTGCGG AGATTAAGAA GCCTCTGAGG
    TGCCGGGAAC CTGAATTTGC TCTCCAGTAC AACGTGCTCG CGCACCCTCG AATTGCTGAT
    GCTGGAGATG GTCTTGTAAG GGCAATGCCG AAAGCAGTGG GAGTGGGAAA TGAACTCACT
    GAACCAGCGG CTGAAATAAT CCGGGCGTAT TTCCCAGAGA CGTGGATCTG GCAGCTAGAG
    CCTGTGGGTG AGTCGGGTAG AGCCGAGGTC CATGGCTCAG CCCCAGACAC CATCACCGAG
    TGGAATGCAG GAGCTTTCTG TATGGGACCC AGTGGGTTTG GCATCTCCCC ACCAACCTCC
    CTTCGAGCCT TCCAGCCCTT CTTTGTGGAG CTCACTCTGC CGTACTCTGT GGTGCGAGGG
    GAGTCATTCA CTCTCAAGGC CTCTGTGTTT AACTACCTCA AGCAGTGTAT AAAGGTTCAG
    ACTACACTCC AGCCGTCACT TGAGTTAGAG CAGGAACCAT GTGCTGACTG TCAGTATAGC
    AGCTGTATCT GTGCAGACGA AAGCAAGACC TTCTACTGGA ACCTTAAAGC CTCCAACCTG
    GGAGAGGTGA ACATCACGGT GAGGACGGAG GCTCTGAACA CTCAGGACAT GTGCAATAAT
    GAGATTCCTA TTGTTCCCAA GCAGGGTCAC TCCGACACTG TGATTAAGCC ACTGCTGGTG
    CAGCCGGGGG GAGTCCTAAT AGAGAAATCT CACAGCTCCC TGATCTGCAG CCCAGAGGGA
    GAAGACCATT CAAAGACAGA GGACATATCC CTGAATGTTC CAGAGAACAT CCTGGAGGGT
    TCTGAGAGAG CGTATGTAAC AGCACTGGGA GACCTGATGG GAACAGCCAT GCAGAACCTG
    GACCGGCTCC TGGCCATGCC ATATGGATGT GGGGAGCAGA ACATGGTTCT CTTTGCCCCC
    AATATCTTCA TTCTTCAGTA TCTGGAAAAC ACTCACCAAC TGAGCCCTGA GATCCAGACC
    AAAGCCAAAG GATTCCTTGA AAGCGGGTAC CAAAGACAAC TGACTTACAA ACGTAACGAT
    GGGTCCTACA GCGCATTTGG GTCAAGGGAC CCCGAGGGAA ACACATGGCT GACTGCATTT
    GTAGTGAAGT CCTTCAGCAA GGCTCGTCCC TACATCTTCA TCGACGGAGA CCACCTAAAC
    CATTCTTTCT CATGGCTAAA AGATAAACGC CAAAGGAGCG GCTGCTTCCG TAGTGTGGGG
    AAACTCTTCA ATAATGCAAT GAAGGGGGGA GTGGATGATG AAATATCTCT TTCTGCTTAT
    GTCACCATTG CTCTAATGGA AGCCGGTCTG TCCAAAGAGG ACCCAATGGT TAGTGATGCT
    TTATCCTGCC TGAGGAAGGC TGCCAGAAAA GTGAGCAGTG CCTACACCCA GGCTCTGCTG
    GCCTACACTT TCACTCTGAG TGATGATACT AAACACAGGG AGAAGCTGCT GGCTAAACTG
    GAGGAGAAAG CTGTGAGGAA AGATGGACAA CTCCACTGGG AGAGGAAACC TGCCTCTAAG
    GCCTCTGACC TCCCATACTG GTACCGCGCT CCCTCAGCCG AGGTGGAGTT AACCTCCTAT
    GCGCTGCTGG CCCTTCTCTC TGGCCCCAAC AAGGACCTGG GCAAAGCTTC TGAGATAGTC
    AACTGGCTGA GCAAGCAGCA AAATCCTTAT GGGGGGTTCT CTTCTACTCA GGACACGGTT
    GTAGCGCTAC AGGCTCTTGC TGAGTATGCC GAGGCCACAT TCTCTGACAA GGGAGATGTC
    ACAGTCACAG TCACTTCCAA GACTGGGTTC CATCAACAGT TCCATGTGGA TCAGACTAAC
    CGGCTCCTGC TACAGAAAGC CTCACTGCCA GATATCCCTG GAGAGTACTC TCTGTCAGCC
    ACTGGGAGCG GCTGTGTGTA TGTACAGACT GTCCTGAGAT ACAACATCCC CCCACCTAGA
    AGTGACGCCA CCTTCTCAGT ACGAGTGGAG ACCCAACCAC AGCAATGTCC CCAGGAGCCA
    ATGAAACAGA TGGGCATAGA CATTTACATT CAATACACAG GGTCTCGGGA AAAATCCAAC
    ATGGCTCTGG TTGAGGTCAA GATGCTCTCA GGCTTTACCC CTGATAAGAA GAGTATTGAC
    CAGGTCATCA GATGGAACAA CTTTATAATG TGGAGTGAGA CCCAAACTGA CAAGGTGACA
    TTCTACTTAA GCGAGTTAGG ACGTCATCCA GTTAATTTCT CCTTCATGGT GGAACAAGAG
    GTTCTGGTGA AGAATCTCAG ACCAGCCATA GTGAAAGTCT ATGATTATTA TGAGCCAGTG
    GAACATGCAG TTACCGAGTA CAACTCCCCC TGCAGCTCGG ATGAAGACAC GGGCAACATA
    ATACAAGGAG TAGAAGCGCC TCGCTATATC AGAGATCCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760642.1
    CDS230..4591
    Translation

    Target ORF information:

    RefSeq Version XM_031904782.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCTGCC ATAGCTCCGA ACAAGCCTGT GTGCACCTCA GTGGGGCGGA GGGAGACAGC 
    CGGATACAGG TTACCTTGAA CATGGCCGAA CGGAACTCAA CGGTGATAGA GAGGAACCTC
    CGGCAGAAAT CCCTCTTCAG CTGTGTTACA TTCCAGGTGC CTCCGCCCTC TGAAGGGAAT
    GAAGAAGTGG CCACCATGGA AATTCTCATT GAGAGCGCAG GGGAGACCAT AACCAACAGC
    AGCAAAGTTC TAGTGAAGAG GAGAAGAACC AGTACTTTCA TTCAGACGGA CAAAGCTGTA
    TATAAACCAG GGCAAACAGT ACGATTCCGA GTGGTTTCTC TTAAAGAAAA TCTACAACCT
    GGAGAAAATC AGATTTCAGT CATCCAATTA CAGGATCCAA ACAAGAACCG TATTGCCCAG
    TGGCTGGATG TGACCCTCAC ACAGGGAATC GCTGAGCTCT CACTGCCGCT GTCCCCGGAA
    CCTTCGCTTG GAGAATATTC CATTCACGTG GGGGACACTG TGCACTACTT TAGCGTGGAA
    GAATACGTCC TTCCCAAGTT TGAGGTCAGT TTCCAGTTCC CCAAGGTGGT GTTCTTTACC
    AGCGAACAAT TCCAGCTGAA AACATGTGGC AGATACACCT ATGGCAAACC TGTGCAAGGA
    ACCTATAAGG CTACGGTGTG CCGTAAGGTC ATAAGATACT GGTGGGGACA CAATGATGAC
    CGCTCAGACC TGTGCGTCGA TTTCAGCGGG AAGTTGGACC AGTCTGGCTG TTCCACATTG
    ACAGCGACGT CTGAAAACTT CAATCTTACC TACAACACTC ATGAAATGGG CCTAGAGGGG
    ACGTCTACTA TTACAGAGGG AGGGACAGGA GTGGAATATT CTGGAAGCGG TAAAACCATC
    ATTTCGTCTG TAGTCACGAG TTGCAGTTTC CTGGATGCTG ACAGTAACTA CAAGCCGGGG
    ATCCCATACA GCGGCACAGT AAAGGTGGTA GATGCCAATG ATGACCCCAT CCCCAACCAG
    ATAGTCTATC TAACCAGCCA TTCTAATGAA GTAAACAAGA CATTGGTGTC GGATGAGAAT
    GGACTCGCCT CCTTCCAGCT GGAGAACACG GCAGAGTGGA AGGGACAGCT GAGCCTTAGG
    GCCAGTACAA CTACACAGGA CGCTGGATTT GATTTCCTTC AGGGCCGTCT CATTACCCCT
    AATGATGTGA GTGCATCGCT CATGCTGAAA CCCTTCCACT CCCCCAGCAA GAGTTTCCTG
    AAACTGCACT CCCAGCAGGG GGCGCTCCCA TGTGAGGGGC AACGAGATGT CCGAGTGGAA
    TATATTATCC CAAACTCTGC CCTGGGGATT GAGGTCAAGC ACATGGAGCT GCACTACCTG
    GTTGCTTCTA AAGGGTCAGT AAAGGACTCG GGATCAGTGG AAGTGCCCAT TACTAGTGAA
    GGAGGAGGAT CTTCAGAGCT GAGGGGTGAG GTCTCCATCA AACTCACACT CAGTGCCGAC
    GTCTCTCCGA CTCTCCGCGC TCTCGCCTTC ATTTCCCTCC CTAATGGGGA ATTAGTGGCC
    GATTCTGCAA CATTCAAACT GCAAAGGTGC TTCAAGAACA AAGTATCAGT TGGCTTCTCC
    CCAGATGAAG TTCTCCCAGG AGCTGATGTT TCCCTACAAG TCCAGGCAGC CGCCGGGTCC
    CTGTGTGGCC TGAGAGTGGT GGATCAGAGT GTGGTGTTAA TGAAACCTGA CAAAGAACTG
    ACGGCCGATC AGATTCACAA TCTCTTTCCT TTCCCTGATT TCGGAGGGTA CGATGCTCGG
    GTCCAAGATA GTGATGATCA TTGTCCATTT TATCCCTGGT TTCATCGACC AATGTGGAAG
    AGAAGTATTG TTCTACCACC GTGGCATATG CCTCATGAAG ATGAGGTTGA TGTATACAGC
    TTATTTAAGG ACATGCGCCT TAAGATCGTC ACTAGTGCGG AGATTAAGAA GCCTCTGAGG
    TGCCGGGAAC CTGAATTTGC TCTCCAGTAC AACGTGCTCG CGCACCCTCG AATTGCTGAT
    GCTGGAGATG GTCTTGTAAG GGCAATGCCG AAAGCAGTGG GAGTGGGAAA TGAACTCACT
    GAACCAGCGG CTGAAATAAT CCGGGCGTAT TTCCCAGAGA CGTGGATCTG GCAGCTAGAG
    CCTGTGGGTG AGTCGGGTAG AGCCGAGGTC CATGGCTCAG CCCCAGACAC CATCACCGAG
    TGGAATGCAG GAGCTTTCTG TATGGGACCC AGTGGGTTTG GCATCTCCCC ACCAACCTCC
    CTTCGAGCCT TCCAGCCCTT CTTTGTGGAG CTCACTCTGC CGTACTCTGT GGTGCGAGGG
    GAGTCATTCA CTCTCAAGGC CTCTGTGTTT AACTACCTCA AGCAGTGTAT AAAGGTTCAG
    ACTACACTCC AGCCGTCACT TGAGTTAGAG CAGGAACCAT GTGCTGACTG TCAGTATAGC
    AGCTGTATCT GTGCAGACGA AAGCAAGACC TTCTACTGGA ACCTTAAAGC CTCCAACCTG
    GGAGAGGTGA ACATCACGGT GAGGACGGAG GCTCTGAACA CTCAGGACAT GTGCAATAAT
    GAGATTCCTA TTGTTCCCAA GCAGGGTCAC TCCGACACTG TGATTAAGCC ACTGCTGGTG
    CAGCCGGGGG GAGTCCTAAT AGAGAAATCT CACAGCTCCC TGATCTGCAG CCCAGAGGGA
    GAAGACCATT CAAAGACAGA GGACATATCC CTGAATGTTC CAGAGAACAT CCTGGAGGGT
    TCTGAGAGAG CGTATGTAAC AGCACTGGGA GACCTGATGG GAACAGCCAT GCAGAACCTG
    GACCGGCTCC TGGCCATGCC ATATGGATGT GGGGAGCAGA ACATGGTTCT CTTTGCCCCC
    AATATCTTCA TTCTTCAGTA TCTGGAAAAC ACTCACCAAC TGAGCCCTGA GATCCAGACC
    AAAGCCAAAG GATTCCTTGA AAGCGGGTAC CAAAGACAAC TGACTTACAA ACGTAACGAT
    GGGTCCTACA GCGCATTTGG GTCAAGGGAC CCCGAGGGAA ACACATGGCT GACTGCATTT
    GTAGTGAAGT CCTTCAGCAA GGCTCGTCCC TACATCTTCA TCGACGGAGA CCACCTAAAC
    CATTCTTTCT CATGGCTAAA AGATAAACGC CAAAGGAGCG GCTGCTTCCG TAGTGTGGGG
    AAACTCTTCA ATAATGCAAT GAAGGGGGGA GTGGATGATG AAATATCTCT TTCTGCTTAT
    GTCACCATTG CTCTAATGGA AGCCGGTCTG TCCAAAGAGG ACCCAATGGT TAGTGATGCT
    TTATCCTGCC TGAGGAAGGC TGCCAGAAAA GTGAGCAGTG CCTACACCCA GGCTCTGCTG
    GCCTACACTT TCACTCTGAG TGATGATACT AAACACAGGG AGAAGCTGCT GGCTAAACTG
    GAGGAGAAAG CTGTGAGGAA AGATGGACAA CTCCACTGGG AGAGGAAACC TGCCTCTAAG
    GCCTCTGACC TCCCATACTG GTACCGCGCT CCCTCAGCCG AGGTGGAGTT AACCTCCTAT
    GCGCTGCTGG CCCTTCTCTC TGGCCCCAAC AAGGACCTGG GCAAAGCTTC TGAGATAGTC
    AACTGGCTGA GCAAGCAGCA AAATCCTTAT GGGGGGTTCT CTTCTACTCA GGACACGGTT
    GTAGCGCTAC AGGCTCTTGC TGAGTATGCC GAGGCCACAT TCTCTGACAA GGGAGATGTC
    ACAGTCACAG TCACTTCCAA GACTGGGTTC CATCAACAGT TCCATGTGGA TCAGACTAAC
    CGGCTCCTGC TACAGAAAGC CTCACTGCCA GATATCCCTG GAGAGTACTC TCTGTCAGCC
    ACTGGGAGCG GCTGTGTGTA TGTACAGACT GTCCTGAGAT ACAACATCCC CCCACCTAGA
    AGTGACGCCA CCTTCTCAGT ACGAGTGGAG ACCCAACCAC AGCAATGTCC CCAGGAGCCA
    ATGAAACAGA TGGGCATAGA CATTTACATT CAATACACAG GGTCTCGGGA AAAATCCAAC
    ATGGCTCTGG TTGAGGTCAA GATGCTCTCA GGCTTTACCC CTGATAAGAA GAGTATTGAC
    CAGGTCATCA GATGGAACAA CTTTATAATG TGGAGTGAGA CCCAAACTGA CAAGGTGACA
    TTCTACTTAA GCGAGTTAGG ACGTCATCCA GTTAATTTCT CCTTCATGGT GGAACAAGAG
    GTTCTGGTGA AGAATCTCAG ACCAGCCATA GTGAAAGTCT ATGATTATTA TGAGCCAGTG
    GAACATGCAG TTACCGAGTA CAACTCCCCC TGCAGCTCGG ATGAAGACAC GGGCAACATA
    ATACAAGGAG TAGAAGCGCC TCGCTATATC AGAGATCCAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61183
    Clone ID Related Accession (Same CDS sequence) XM_031904783.1
    Accession Version XM_031904783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCTCTACA GGCTGTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTTTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTATATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC TGATAAGAAA AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC ATAGTGACAT TCTACTTAAG CGAGGACGTC
    ATCCAATTAA TTTCTCCTTC ATGGTGGAAC AAGAGATTCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760643.1
    CDS182..4486
    Translation

    Target ORF information:

    RefSeq Version XM_031904783.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCTCTACA GGCTGTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTTTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTATATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC TGATAAGAAA AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC ATAGTGACAT TCTACTTAAG CGAGGACGTC
    ATCCAATTAA TTTCTCCTTC ATGGTGGAAC AAGAGATTCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61184
    Clone ID Related Accession (Same CDS sequence) XM_031904785.1 , XM_031904784.1
    Accession Version XM_031904785.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGGACGTC
    ATCCAGTTAA TTTCTCCTTC ATGGTGGAAC AAGAGGTTCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760645.1
    CDS184..4488
    Translation

    Target ORF information:

    RefSeq Version XM_031904785.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGGACGTC
    ATCCAGTTAA TTTCTCCTTC ATGGTGGAAC AAGAGGTTCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61184
    Clone ID Related Accession (Same CDS sequence) XM_031904785.1 , XM_031904784.1
    Accession Version XM_031904784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGGACGTC
    ATCCAGTTAA TTTCTCCTTC ATGGTGGAAC AAGAGGTTCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031760644.1
    CDS184..4488
    Translation

    Target ORF information:

    RefSeq Version XM_031904784.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031904784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GTACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCTG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA AAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACGGTTG TAGCGCTACA GGCTCTTGCT
    GAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG AGAGTACTCT CTGTCAGCCA CTGGGAGCGG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATCCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCACA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GGGCATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAA AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTACCCC TGATAAGAAG AGTATTGACC AGGTCATCAG ATGGAACAAC
    TTTATAATGT GGAGTGAGAC CCAAACTGAC AAGGTGACAT TCTACTTAAG CGAGGACGTC
    ATCCAGTTAA TTTCTCCTTC ATGGTGGAAC AAGAGGTTCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22824
    Clone ID Related Accession (Same CDS sequence) NM_001113027.1
    Accession Version NM_001113027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154698.1. ##Evidence-Data-START## Transcript exon combination :: BC154698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCTGCC ATAGCTCCGA ACAAGCCTGT GTGCACCTCA GTGGGGCGGA GGGAGACAGC 
    CGGATACAGG TTACCTTGAA CATGGCCGAA CGGAACTCAA CGGTGATAGA GAGGAACCTC
    CAGCAGAAAT CCCTCTTCAG CTGTGTTACA TTCCAGGTGC CTCCGCCCTC TGAAGGGAAT
    GAAGAAGTGG CCACCATGGA AATTCTCATT GAGAGCGCAG GGGAGACCAT AACCAACAGC
    AGCAAAGTTC TAGTGAAGAG GAGAAGAACC AGTACTTTCA TTCAGACGGA CAAAGCTGTA
    TATAAACCAG GGCAAACAGT ACGATTCCGA GTGGTTTCTC TTAAAGAAAA TCTACAACCT
    GGAGAAAGTC AGATTTCAGT CATCCAATTA CAGGATCCAA ACAAGAACCG TATTGCCCAG
    TGGCTGGATG TGACCCTCAC ACAGGGAATC GCTGAGCTCT CACTGCCGCT GTCCCCGGAA
    CCTTCGCTTG GAGAATATTC CATTCACGTG GGGGACACTG TGCACTACTT TAGCGTGGAA
    GAATACGTCC TTCCCAAGTT TGAGGTCAGT TTCCAGTTCC CCAAGGTGGT GTTCTTTACC
    AGCGAACAAT TCCAGCTGAA AACATGTGGC AGATACACCT ATGGCAAACC TGTGCAAGGA
    ACCTATAAGG CTACAGTGTG CCGTAAGGTC ATAAGGTACT GGTGGGGACA CAATGATGAC
    CGCTCAGACC TGTGCGTCAA TTTCAGCGGG AAGTTGGACC AGTCTGGCTG TTCCACATTG
    ACAGCGACGT CTGAAAACTT CAATCTTACC TACAACACTC ATGAAATGGG CCTAGAGGGG
    AGGTCTACTA TTACAGAGGG AGGGACAGGA GTGGAATATT CTGGAAGCGG TAAAACCATC
    ATTTCGTCTT TAGTCACGAG TTGCAGTTTC CTGGATGCTG ACAGTAACTA CAAGCCGGGG
    ATCCCATACA GCGGCACAGT AAAGGTGGTA GATGCCAATG ATGACCCCAT CCCCAACCAG
    ATAGTCTATC TAACCAGCCA TTCTAATGAA GTAAACAAGA CGTTGGTGTC GGATGAGAAT
    GGACTCGCCT CCTTCCAGCT GGAGAACACG GCAGAGTGGA AGGGACAGCT GAGCCTTAGG
    GCCAGTACAA CTACACAGGA CGCTGGATTT GATTTCCTTC AGGGCCGTCT CATTACCCCT
    AATGATGTGA GTGCATCGCT CATGCTGAAA CCCTTCCACT CCCCCAGCAA GAGTTTCCTG
    AAACTGCACT CCCAGCAGGG GGCGCTCCCA TGTGAGGGGC AACGAGATGT CCGAGTGGAA
    TATATTATCC CAAACTCTGC CCTGGGGATG GAGGTCAAGC ACATGGAGCT GCACTACCTG
    GTTGCTTCTA AAGGGTCAGT AAAGGACTCG GGATCAGTGG AAGTGCCCAT TACTAGTGAA
    GGAGGAGGAT CTTCAGAGCT GAGGGGTGAG GTCTCCATCA AACTCACACT CAGTGCCGAC
    GTCTCTCCGA CTCTCCGCGC TCTCGCCTTC ATTTCCCTTC CTAATGGGGA ATTAGTGGCC
    GATTCTGCAA CATTCAAACT GCAAAGGTGC TTCAAGAACA AAGTATCAGT TGGCTTCTCC
    CCAGATGAAG TTCTCCCAGG AGCTGATGTT TCCCTACAAG TCCAGGCAGC CGCCGGGTCC
    CTGTGTGGCC TGAGAGTGGT GGATCAGAGT GTGGTGTTAA TGAAACCTGA CAAAGAACTG
    ACGGACGACC AGATTCACAA TCTCTTTCCT TTCCCTGATT TCGGAGGGTA CGATGCTCGA
    GTCCAAGATA GTGATGATCA TTGTCCATTT TATTCCTGGT TTCATCGACC AATGTGGAAG
    AGAAGTATTG TTCTACCACC GTGGCATATG CCTCATGAAG ATGAAGTTGA TGTATACAGC
    TTATTTAAGG ACATGCGCCT TAAGATCGTC ACTAGTGCGG AGATTAAGAA GCCTCTGAGG
    TGCCGGGAAC CTGAATTTGC TCTCCAGTAC AACGTGCTCG CGCACCCTCG AATTGCTGAT
    GCTGGAGATG GTCTTGTAAG GGCAATGCCG AAAGCAGTGG GAGTGGGAAA TGAACTCACT
    GAACCAGCGG CTGAAATAAT CCGGGCGTAT TTCCCAGAGA CGTGGATCTG GCAGCTAGAG
    CCTGTGGGTG AGTCGGGTAG AGCCGAGGTC CATGGCTCAG CCCCAGACAC CATCACCGAG
    TGGAATGCAG GAGCTTTCTG TATGGGACCC AGTGGGTTTG GCATCTCCCC ACCAACCTCC
    CTTCGAGCCT TCCAGCCCTT CTTTGTGGAG CTCACTCTGC CGTACTCTGT GGTGCGAGGG
    GAGTCATTCA CTCTCAAGGC CTCTGTGTTT AACTACCTCA AGCAGTGTAT AAAGGTTCAG
    ACTACACTCC AGCCGTCACT TGAGTTAGAG CAGGAACCAT GTGCTGACTG TCAGTATAGC
    AGCTGTATCT GTGCAGACGA AAGCAAGACC TTCTACTGGA ACCTTAAAGC CTCCAACCTG
    GGAGAGGTGA ACATCACGGT GAGGACGGAG GCTCTGAACA CTCAGGACAT GTGCAATAAT
    GAGATTCCTA TTGTTCCCAA GCAGGGTCAC TCCGACACTG TGATTAAGCC ACTGCTGGTG
    CAGCCGGGGG GAGTCCTAAT AGAGAAATCT CACAGCTCCC TGATCTGCAG CCCAGAGGGA
    GAAGACCATT CTAAGACAGA GGACATATCC CTGAATGTTC CAGAGAACAT CCTGGAGGGT
    TCTGAGAGAG CGTATGTAAC AGCACTGGGA GACCTGATGG GAACAGCCAT GCAGAACCTG
    GACCGGCTCC TGGCCATGCC ATATGGATGT GGGGAGCAGA ACATGGTTCT CTTTGCCCCC
    AATATCTTCA TTCTTCAGTA TCTGGAAAAC ACTCACCAAC TGAGCCCTGA GATCCAGAGC
    AAAGCCAAAG GATTCCTTGA AAGCGGGTAC CAAAGACAAC TGACTTACAA ACGTAACGAT
    GGGTCCTACA GCGCATTTGG GTCAAGGGAC CCCGAGGGAA ACACATGGCT GACTGCATTT
    GTAGTGAAGT CCTTCAGCAA GGCTCGTCCC TACATCTTCA TCGACGGAGA CCACCTAAAC
    CATTCTTTCT CATGGCTAAA AGATAAACGC CAAAGGAGCG GCTGCTTCCG TAGTGTGGGG
    AAACTCTTCA ATAATGCAAT GAAGGGGGGA GTGGATGATG AAATATCTCT TTCTGCTTAT
    GTCACCATTG CTCTAATGGA AGCCGGTCTG TCCAAAGAGG ACCCAATGGT TAGTGATGCT
    TTATCCTGCC TGAGGAAGGC TGCCAGAAAA GTGAGCAGTG CCTACACCCA GGCTCTGCTG
    GCCTACACTT TCACTCTGAG TGATGATACT AAACACAGGG AGAAGCTGCT GGCTAAACTG
    GAGGAGAAAG CTGTGAGGAA AGATGGACAA CTCCACTGGG AGAGGAAACC TGCCTCTGAG
    GCCTCTGACC TCCCATACTG GTACCGCGCT CCCTCAGCCG AGGTGGAGTT AACCTCCTAT
    GTGCTGCTGG CCCTTCTCTC TGGCCCCAAC AAGGACCTGG GCAAAGCTTC TGAGATAGTC
    AACTGGCTGA GCAAGCAGCA AAATCCTTAT GGGGGGTTCT CTTCTACTCA GGACACAGTT
    GTAGCTCTAC AGGCTCTTGC TAAGTATGCC GAGGCCACAT TCTCTGACAA GGGAGATGTC
    ACAGTCACAG TCACTTCCAA GACTGGGTTC CATCAACAGT TCCATGTGGA TCAGACTAAC
    CGGCTCCTGC TACAGAAAGC CTCACTGCCA GATATCCCTG GGGAGTACTC TCTGTCAGCC
    ACTGGGAGCA GCTGTGTGTA TGTACAGACT GTCCTGAGAT ACAACATTCC CCCACCTAGA
    AGTGACGCCA CCTTCTCAGT ACGAGTGGAG ACCCAACCAA AGCAATGTCC CCAGGAGCCA
    ATGAAACAGA TGCACATAGA CATTTACATT CAATACACAG GGTCTCGGGA GAAATCCAAC
    ATGGCTCTGG TTGAGGTCAA GATGCTCTCA GGCTTTATCC CAGTGAAGAG CACAATAAAG
    CAGCTAGTGA GCAGCAATAC GATAAAGCGG AGTGAGATCC AAACTGATAT GGTGACTTTG
    TACCTGAATG AGTTAGGACA TGATCCAGTT CACTTCTCGA TTATGGTGGA ACAAGACATT
    GAGGTGAAGA ACCTAAAGCC GGCCACGGTG AAGGTCTATG ATTATTATGA GACAGCGGAA
    CATGCAGTTA CCGAGTACAA CTCCCCCTGC AGCTCGGATA AAGAAGCAGA AAACACAAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001106498.1
    CDS337..4659
    Translation

    Target ORF information:

    RefSeq Version NM_001113027.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001113027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGCTGCC ATAGCTCCGA ACAAGCCTGT GTGCACCTCA GTGGGGCGGA GGGAGACAGC 
    CGGATACAGG TTACCTTGAA CATGGCCGAA CGGAACTCAA CGGTGATAGA GAGGAACCTC
    CAGCAGAAAT CCCTCTTCAG CTGTGTTACA TTCCAGGTGC CTCCGCCCTC TGAAGGGAAT
    GAAGAAGTGG CCACCATGGA AATTCTCATT GAGAGCGCAG GGGAGACCAT AACCAACAGC
    AGCAAAGTTC TAGTGAAGAG GAGAAGAACC AGTACTTTCA TTCAGACGGA CAAAGCTGTA
    TATAAACCAG GGCAAACAGT ACGATTCCGA GTGGTTTCTC TTAAAGAAAA TCTACAACCT
    GGAGAAAGTC AGATTTCAGT CATCCAATTA CAGGATCCAA ACAAGAACCG TATTGCCCAG
    TGGCTGGATG TGACCCTCAC ACAGGGAATC GCTGAGCTCT CACTGCCGCT GTCCCCGGAA
    CCTTCGCTTG GAGAATATTC CATTCACGTG GGGGACACTG TGCACTACTT TAGCGTGGAA
    GAATACGTCC TTCCCAAGTT TGAGGTCAGT TTCCAGTTCC CCAAGGTGGT GTTCTTTACC
    AGCGAACAAT TCCAGCTGAA AACATGTGGC AGATACACCT ATGGCAAACC TGTGCAAGGA
    ACCTATAAGG CTACAGTGTG CCGTAAGGTC ATAAGGTACT GGTGGGGACA CAATGATGAC
    CGCTCAGACC TGTGCGTCAA TTTCAGCGGG AAGTTGGACC AGTCTGGCTG TTCCACATTG
    ACAGCGACGT CTGAAAACTT CAATCTTACC TACAACACTC ATGAAATGGG CCTAGAGGGG
    AGGTCTACTA TTACAGAGGG AGGGACAGGA GTGGAATATT CTGGAAGCGG TAAAACCATC
    ATTTCGTCTT TAGTCACGAG TTGCAGTTTC CTGGATGCTG ACAGTAACTA CAAGCCGGGG
    ATCCCATACA GCGGCACAGT AAAGGTGGTA GATGCCAATG ATGACCCCAT CCCCAACCAG
    ATAGTCTATC TAACCAGCCA TTCTAATGAA GTAAACAAGA CGTTGGTGTC GGATGAGAAT
    GGACTCGCCT CCTTCCAGCT GGAGAACACG GCAGAGTGGA AGGGACAGCT GAGCCTTAGG
    GCCAGTACAA CTACACAGGA CGCTGGATTT GATTTCCTTC AGGGCCGTCT CATTACCCCT
    AATGATGTGA GTGCATCGCT CATGCTGAAA CCCTTCCACT CCCCCAGCAA GAGTTTCCTG
    AAACTGCACT CCCAGCAGGG GGCGCTCCCA TGTGAGGGGC AACGAGATGT CCGAGTGGAA
    TATATTATCC CAAACTCTGC CCTGGGGATG GAGGTCAAGC ACATGGAGCT GCACTACCTG
    GTTGCTTCTA AAGGGTCAGT AAAGGACTCG GGATCAGTGG AAGTGCCCAT TACTAGTGAA
    GGAGGAGGAT CTTCAGAGCT GAGGGGTGAG GTCTCCATCA AACTCACACT CAGTGCCGAC
    GTCTCTCCGA CTCTCCGCGC TCTCGCCTTC ATTTCCCTTC CTAATGGGGA ATTAGTGGCC
    GATTCTGCAA CATTCAAACT GCAAAGGTGC TTCAAGAACA AAGTATCAGT TGGCTTCTCC
    CCAGATGAAG TTCTCCCAGG AGCTGATGTT TCCCTACAAG TCCAGGCAGC CGCCGGGTCC
    CTGTGTGGCC TGAGAGTGGT GGATCAGAGT GTGGTGTTAA TGAAACCTGA CAAAGAACTG
    ACGGACGACC AGATTCACAA TCTCTTTCCT TTCCCTGATT TCGGAGGGTA CGATGCTCGA
    GTCCAAGATA GTGATGATCA TTGTCCATTT TATTCCTGGT TTCATCGACC AATGTGGAAG
    AGAAGTATTG TTCTACCACC GTGGCATATG CCTCATGAAG ATGAAGTTGA TGTATACAGC
    TTATTTAAGG ACATGCGCCT TAAGATCGTC ACTAGTGCGG AGATTAAGAA GCCTCTGAGG
    TGCCGGGAAC CTGAATTTGC TCTCCAGTAC AACGTGCTCG CGCACCCTCG AATTGCTGAT
    GCTGGAGATG GTCTTGTAAG GGCAATGCCG AAAGCAGTGG GAGTGGGAAA TGAACTCACT
    GAACCAGCGG CTGAAATAAT CCGGGCGTAT TTCCCAGAGA CGTGGATCTG GCAGCTAGAG
    CCTGTGGGTG AGTCGGGTAG AGCCGAGGTC CATGGCTCAG CCCCAGACAC CATCACCGAG
    TGGAATGCAG GAGCTTTCTG TATGGGACCC AGTGGGTTTG GCATCTCCCC ACCAACCTCC
    CTTCGAGCCT TCCAGCCCTT CTTTGTGGAG CTCACTCTGC CGTACTCTGT GGTGCGAGGG
    GAGTCATTCA CTCTCAAGGC CTCTGTGTTT AACTACCTCA AGCAGTGTAT AAAGGTTCAG
    ACTACACTCC AGCCGTCACT TGAGTTAGAG CAGGAACCAT GTGCTGACTG TCAGTATAGC
    AGCTGTATCT GTGCAGACGA AAGCAAGACC TTCTACTGGA ACCTTAAAGC CTCCAACCTG
    GGAGAGGTGA ACATCACGGT GAGGACGGAG GCTCTGAACA CTCAGGACAT GTGCAATAAT
    GAGATTCCTA TTGTTCCCAA GCAGGGTCAC TCCGACACTG TGATTAAGCC ACTGCTGGTG
    CAGCCGGGGG GAGTCCTAAT AGAGAAATCT CACAGCTCCC TGATCTGCAG CCCAGAGGGA
    GAAGACCATT CTAAGACAGA GGACATATCC CTGAATGTTC CAGAGAACAT CCTGGAGGGT
    TCTGAGAGAG CGTATGTAAC AGCACTGGGA GACCTGATGG GAACAGCCAT GCAGAACCTG
    GACCGGCTCC TGGCCATGCC ATATGGATGT GGGGAGCAGA ACATGGTTCT CTTTGCCCCC
    AATATCTTCA TTCTTCAGTA TCTGGAAAAC ACTCACCAAC TGAGCCCTGA GATCCAGAGC
    AAAGCCAAAG GATTCCTTGA AAGCGGGTAC CAAAGACAAC TGACTTACAA ACGTAACGAT
    GGGTCCTACA GCGCATTTGG GTCAAGGGAC CCCGAGGGAA ACACATGGCT GACTGCATTT
    GTAGTGAAGT CCTTCAGCAA GGCTCGTCCC TACATCTTCA TCGACGGAGA CCACCTAAAC
    CATTCTTTCT CATGGCTAAA AGATAAACGC CAAAGGAGCG GCTGCTTCCG TAGTGTGGGG
    AAACTCTTCA ATAATGCAAT GAAGGGGGGA GTGGATGATG AAATATCTCT TTCTGCTTAT
    GTCACCATTG CTCTAATGGA AGCCGGTCTG TCCAAAGAGG ACCCAATGGT TAGTGATGCT
    TTATCCTGCC TGAGGAAGGC TGCCAGAAAA GTGAGCAGTG CCTACACCCA GGCTCTGCTG
    GCCTACACTT TCACTCTGAG TGATGATACT AAACACAGGG AGAAGCTGCT GGCTAAACTG
    GAGGAGAAAG CTGTGAGGAA AGATGGACAA CTCCACTGGG AGAGGAAACC TGCCTCTGAG
    GCCTCTGACC TCCCATACTG GTACCGCGCT CCCTCAGCCG AGGTGGAGTT AACCTCCTAT
    GTGCTGCTGG CCCTTCTCTC TGGCCCCAAC AAGGACCTGG GCAAAGCTTC TGAGATAGTC
    AACTGGCTGA GCAAGCAGCA AAATCCTTAT GGGGGGTTCT CTTCTACTCA GGACACAGTT
    GTAGCTCTAC AGGCTCTTGC TAAGTATGCC GAGGCCACAT TCTCTGACAA GGGAGATGTC
    ACAGTCACAG TCACTTCCAA GACTGGGTTC CATCAACAGT TCCATGTGGA TCAGACTAAC
    CGGCTCCTGC TACAGAAAGC CTCACTGCCA GATATCCCTG GGGAGTACTC TCTGTCAGCC
    ACTGGGAGCA GCTGTGTGTA TGTACAGACT GTCCTGAGAT ACAACATTCC CCCACCTAGA
    AGTGACGCCA CCTTCTCAGT ACGAGTGGAG ACCCAACCAA AGCAATGTCC CCAGGAGCCA
    ATGAAACAGA TGCACATAGA CATTTACATT CAATACACAG GGTCTCGGGA GAAATCCAAC
    ATGGCTCTGG TTGAGGTCAA GATGCTCTCA GGCTTTATCC CAGTGAAGAG CACAATAAAG
    CAGCTAGTGA GCAGCAATAC GATAAAGCGG AGTGAGATCC AAACTGATAT GGTGACTTTG
    TACCTGAATG AGTTAGGACA TGATCCAGTT CACTTCTCGA TTATGGTGGA ACAAGACATT
    GAGGTGAAGA ACCTAAAGCC GGCCACGGTG AAGGTCTATG ATTATTATGA GACAGCGGAA
    CATGCAGTTA CCGAGTACAA CTCCCCCTGC AGCTCGGATA AAGAAGCAGA AAACACAAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31002
    Clone ID Related Accession (Same CDS sequence) XM_012954234.2
    Accession Version XM_012954234.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GCACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCGG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA GAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACAGTTG TAGCTCTACA GGCTCTTGCT
    AAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCAG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCAAA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAG AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC CGTGAAGAGC ACAATAAAGC AGCTAGTGAG CAGCAATACG
    ATAAAGCGGA GTGAGATCCA AACTGATATG GTGACTTTGT ACCTGAATGA GTTAGGACAT
    GATCCAGTTC ACTTCTCGAT TATGGTGGAA CAAGACATTG AGGTGAAGAA CCTAAAGCCG
    GCCACGGTGA AGGTCTATGA TTATTATGAG ACAGCGGAAC ATGCAGTTAC CGAGTACAAC
    TCCCCCTGCA GCTCGGATAA AGAAGCAGAA AACACAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809688.1
    CDS131..4552
    Translation

    Target ORF information:

    RefSeq Version XM_012954234.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GCACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCGG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTGAG TGCATCGCTC
    ATGCTGAAAC CCTTCCACTC CCCCAGCAAG AGTTTCCTGA AACTGCACTC CCAGCAGGGG
    GCGCTCCCAT GTGAGGGGCA ACGAGATGTC CGAGTGGAAT ATATTATCCC AAACTCTGCC
    CTGGGGATTG AGGTCAAGCA CATGGAGCTG CACTACCTGG TTGCTTCTAA AGGGTCAGTA
    AAGGACTCGG GATCAGTGGA AGTGCCCATT ACTAGTGAAG GAGGAGGATC TTCAGAGCTG
    AGGGGTGAGG TCTCCATCAA ACTCACACTC AGTGCCGACG TCTCTCCGAC TCTCCGCGCT
    CTCGCCTTCA TTTCCCTCCC TAATGGGGAA TTAGTGGCCG ATTCTGCAAC ATTCAAACTG
    CAAAGGTGCT TCAAGAACAA AGTATCAGTT GGCTTCTCCC CAGATGAAGT TCTCCCAGGA
    GCTGATGTTT CCCTACAAGT CCAGGCAGCC GCCGGGTCCC TGTGTGGCCT GAGAGTGGTG
    GATCAGAGTG TGGTGTTAAT GAAACCTGAC AAAGAACTGA CGGCCGATCA GATTCACAAT
    CTCTTTCCTT TCCCTGATTT CGGAGGGTAC GATGCTCGGG TCCAAGATAG TGATGATCAT
    TGTCCATTTT ATCCCTGGTT TCATCGACCA ATGTGGAAGA GAAGTATTGT TCTACCACCG
    TGGCATATGC CTCATGAAGA TGAGGTTGAT GTATACAGCT TATTTAAGGA CATGCGCCTT
    AAGATCGTCA CTAGTGCGGA GATTAAGAAG CCTCTGAGGT GCCGGGAACC TGAATTTGCT
    CTCCAGTACA ACGTGCTCGC GCACCCTCGA ATTGCTGATG CTGGAGATGG TCTTGTAAGG
    GCAATGCCGA GAGCAGTGGG AGTGGGAAAT GAACTCACTG AACCAGCGGC TGAAATAATC
    CGGGCGTATT TCCCAGAGAC GTGGATCTGG CAGCTAGAGC CTGTGGGTGA GTCGGGTAGA
    GCCGAGGTCC ATGGCTCAGC CCCAGACACC ATCACCGAGT GGAATGCAGG AGCTTTCTGT
    ATGGGACCCA GTGGGTTTGG CATCTCCCCA CCAACCTCCC TTCGAGCCTT CCAGCCCTTC
    TTTGTGGAGC TCACTCTGCC GTACTCTGTG GTGCGAGGGG AGTCATTCAC TCTCAAGGCC
    TCTGTGTTTA ACTACCTCAA GCAGTGTATA AAGGTTCAGA CTACACTCCA GCCGTCACTT
    GAGTTAGAGC AGGAACCATG TGCTGACTGT CAGTATAGCA GCTGTATCTG TGCAGACGAA
    AGCAAGACCT TCTACTGGAA CCTTAAAGCC TCCAACCTGG GAGAGGTGAA CATCACGGTG
    AGGACGGAGG CTCTGAACAC TCAGGACATG TGCAATAATG AGATTCCTAT TGTTCCCAAG
    CAGGGTCACT CCGACACTGT GATTAAGCCA CTGCTGGTGC AGCCGGGGGG AGTCCTAATA
    GAGAAATCTC ACAGCTCCCT GATCTGCAGC CCAGAGGGAG AAGACCATTC AAAGACAGAG
    GACATATCCC TGAATGTTCC AGAGAACATC CTGGAGGGTT CTGAGAGAGC GTATGTAACA
    GCACTGGGAG ACCTGATGGG AACAGCCATG CAGAACCTGG ACCGGCTCCT GGCCATGCCA
    TATGGATGTG GGGAGCAGAA CATGGTTCTC TTTGCCCCCA ATATCTTCAT TCTTCAGTAT
    CTGGAAAACA CTCACCAACT GAGCCCTGAG ATCCAGACCA AAGCCAAAGG ATTCCTTGAA
    AGCGGGTACC AAAGACAACT GACTTACAAA CGTAACGATG GGTCCTACAG CGCATTTGGG
    TCAAGGGACC CCGAGGGAAA CACATGGCTG ACTGCATTTG TAGTGAAGTC CTTCAGCAAG
    GCTCGTCCCT ACATCTTCAT CGACGGAGAC CACCTAAACC ATTCTTTCTC ATGGCTAAAA
    GATAAACGCC AAAGGAGCGG CTGCTTCCGT AGTGTGGGGA AACTCTTCAA TAATGCAATG
    AAGGGGGGAG TGGATGATGA AATATCTCTT TCTGCTTATG TCACCATTGC TCTAATGGAA
    GCCGGTCTGT CCAAAGAGGA CCCAATGGTT AGTGATGCTT TATCCTGCCT GAGGAAGGCT
    GCCAGAAAAG TGAGCAGTGC CTACACCCAG GCTCTGCTGG CCTACACTTT CACTCTGAGT
    GATGATACTA AACACAGGGA GAAGCTGCTG GCTAAACTGG AGGAGAAAGC TGTGAGGAAA
    GATGGACAAC TCCACTGGGA GAGGAAACCT GCCTCTAAGG CCTCTGACCT CCCATACTGG
    TACCGCGCTC CCTCAGCCGA GGTGGAGTTA ACCTCCTATG CGCTGCTGGC CCTTCTCTCT
    GGCCCCAACA AGGACCTGGG CAAAGCTTCT GAGATAGTCA ACTGGCTGAG CAAGCAGCAA
    AATCCTTATG GGGGGTTCTC TTCTACTCAG GACACAGTTG TAGCTCTACA GGCTCTTGCT
    AAGTATGCCG AGGCCACATT CTCTGACAAG GGAGATGTCA CAGTCACAGT CACTTCCAAG
    ACTGGGTTCC ATCAACAGTT CCATGTGGAT CAGACTAACC GGCTCCTGCT ACAGAAAGCC
    TCACTGCCAG ATATCCCTGG GGAGTACTCT CTGTCAGCCA CTGGGAGCAG CTGTGTGTAT
    GTACAGACTG TCCTGAGATA CAACATTCCC CCACCTAGAA GTGACGCCAC CTTCTCAGTA
    CGAGTGGAGA CCCAACCAAA GCAATGTCCC CAGGAGCCAA TGAAACAGAT GCACATAGAC
    ATTTACATTC AATACACAGG GTCTCGGGAG AAATCCAACA TGGCTCTGGT TGAGGTCAAG
    ATGCTCTCAG GCTTTATCCC CGTGAAGAGC ACAATAAAGC AGCTAGTGAG CAGCAATACG
    ATAAAGCGGA GTGAGATCCA AACTGATATG GTGACTTTGT ACCTGAATGA GTTAGGACAT
    GATCCAGTTC ACTTCTCGAT TATGGTGGAA CAAGACATTG AGGTGAAGAA CCTAAAGCCG
    GCCACGGTGA AGGTCTATGA TTATTATGAG ACAGCGGAAC ATGCAGTTAC CGAGTACAAC
    TCCCCCTGCA GCTCGGATAA AGAAGCAGAA AACACAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31003
    Clone ID Related Accession (Same CDS sequence) XM_012954235.2
    Accession Version XM_012954235.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product alpha-2-macroglobulin-like 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683538.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012954235.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GCACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCGG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTTGC TTCTAAAGGG
    TCAGTAAAGG ACTCGGGATC AGTGGAAGTG CCCATTACTA GTGAAGGAGG AGGATCTTCA
    GAGCTGAGGG GTGAGGTCTC CATCAAACTC ACACTCAGTG CCGACGTCTC TCCGACTCTC
    CGCGCTCTCG CCTTCATTTC CCTCCCTAAT GGGGAATTAG TGGCCGATTC TGCAACATTC
    AAACTGCAAA GGTGCTTCAA GAACAAAGTA TCAGTTGGCT TCTCCCCAGA TGAAGTTCTC
    CCAGGAGCTG ATGTTTCCCT ACAAGTCCAG GCAGCCGCCG GGTCCCTGTG TGGCCTGAGA
    GTGGTGGATC AGAGTGTGGT GTTAATGAAA CCTGACAAAG AACTGACGGC CGATCAGATT
    CACAATCTCT TTCCTTTCCC TGATTTCGGA GGGTACGATG CTCGGGTCCA AGATAGTGAT
    GATCATTGTC CATTTTATCC CTGGTTTCAT CGACCAATGT GGAAGAGAAG TATTGTTCTA
    CCACCGTGGC ATATGCCTCA TGAAGATGAG GTTGATGTAT ACAGCTTATT TAAGGACATG
    CGCCTTAAGA TCGTCACTAG TGCGGAGATT AAGAAGCCTC TGAGGTGCCG GGAACCTGAA
    TTTGCTCTCC AGTACAACGT GCTCGCGCAC CCTCGAATTG CTGATGCTGG AGATGGTCTT
    GTAAGGGCAA TGCCGAGAGC AGTGGGAGTG GGAAATGAAC TCACTGAACC AGCGGCTGAA
    ATAATCCGGG CGTATTTCCC AGAGACGTGG ATCTGGCAGC TAGAGCCTGT GGGTGAGTCG
    GGTAGAGCCG AGGTCCATGG CTCAGCCCCA GACACCATCA CCGAGTGGAA TGCAGGAGCT
    TTCTGTATGG GACCCAGTGG GTTTGGCATC TCCCCACCAA CCTCCCTTCG AGCCTTCCAG
    CCCTTCTTTG TGGAGCTCAC TCTGCCGTAC TCTGTGGTGC GAGGGGAGTC ATTCACTCTC
    AAGGCCTCTG TGTTTAACTA CCTCAAGCAG TGTATAAAGG TTCAGACTAC ACTCCAGCCG
    TCACTTGAGT TAGAGCAGGA ACCATGTGCT GACTGTCAGT ATAGCAGCTG TATCTGTGCA
    GACGAAAGCA AGACCTTCTA CTGGAACCTT AAAGCCTCCA ACCTGGGAGA GGTGAACATC
    ACGGTGAGGA CGGAGGCTCT GAACACTCAG GACATGTGCA ATAATGAGAT TCCTATTGTT
    CCCAAGCAGG GTCACTCCGA CACTGTGATT AAGCCACTGC TGGTGCAGCC GGGGGGAGTC
    CTAATAGAGA AATCTCACAG CTCCCTGATC TGCAGCCCAG AGGGAGAAGA CCATTCAAAG
    ACAGAGGACA TATCCCTGAA TGTTCCAGAG AACATCCTGG AGGGTTCTGA GAGAGCGTAT
    GTAACAGCAC TGGGAGACCT GATGGGAACA GCCATGCAGA ACCTGGACCG GCTCCTGGCC
    ATGCCATATG GATGTGGGGA GCAGAACATG GTTCTCTTTG CCCCCAATAT CTTCATTCTT
    CAGTATCTGG AAAACACTCA CCAACTGAGC CCTGAGATCC AGACCAAAGC CAAAGGATTC
    CTTGAAAGCG GGTACCAAAG ACAACTGACT TACAAACGTA ACGATGGGTC CTACAGCGCA
    TTTGGGTCAA GGGACCCCGA GGGAAACACA TGGCTGACTG CATTTGTAGT GAAGTCCTTC
    AGCAAGGCTC GTCCCTACAT CTTCATCGAC GGAGACCACC TAAACCATTC TTTCTCATGG
    CTAAAAGATA AACGCCAAAG GAGCGGCTGC TTCCGTAGTG TGGGGAAACT CTTCAATAAT
    GCAATGAAGG GGGGAGTGGA TGATGAAATA TCTCTTTCTG CTTATGTCAC CATTGCTCTA
    ATGGAAGCCG GTCTGTCCAA AGAGGACCCA ATGGTTAGTG ATGCTTTATC CTGCCTGAGG
    AAGGCTGCCA GAAAAGTGAG CAGTGCCTAC ACCCAGGCTC TGCTGGCCTA CACTTTCACT
    CTGAGTGATG ATACTAAACA CAGGGAGAAG CTGCTGGCTA AACTGGAGGA GAAAGCTGTG
    AGGAAAGATG GACAACTCCA CTGGGAGAGG AAACCTGCCT CTAAGGCCTC TGACCTCCCA
    TACTGGTACC GCGCTCCCTC AGCCGAGGTG GAGTTAACCT CCTATGCGCT GCTGGCCCTT
    CTCTCTGGCC CCAACAAGGA CCTGGGCAAA GCTTCTGAGA TAGTCAACTG GCTGAGCAAG
    CAGCAAAATC CTTATGGGGG GTTCTCTTCT ACTCAGGACA CAGTTGTAGC TCTACAGGCT
    CTTGCTAAGT ATGCCGAGGC CACATTCTCT GACAAGGGAG ATGTCACAGT CACAGTCACT
    TCCAAGACTG GGTTCCATCA ACAGTTCCAT GTGGATCAGA CTAACCGGCT CCTGCTACAG
    AAAGCCTCAC TGCCAGATAT CCCTGGGGAG TACTCTCTGT CAGCCACTGG GAGCAGCTGT
    GTGTATGTAC AGACTGTCCT GAGATACAAC ATTCCCCCAC CTAGAAGTGA CGCCACCTTC
    TCAGTACGAG TGGAGACCCA ACCAAAGCAA TGTCCCCAGG AGCCAATGAA ACAGATGCAC
    ATAGACATTT ACATTCAATA CACAGGGTCT CGGGAGAAAT CCAACATGGC TCTGGTTGAG
    GTCAAGATGC TCTCAGGCTT TATCCCCGTG AAGAGCACAA TAAAGCAGCT AGTGAGCAGC
    AATACGATAA AGCGGAGTGA GATCCAAACT GATATGGTGA CTTTGTACCT GAATGAGTTA
    GGACATGATC CAGTTCACTT CTCGATTATG GTGGAACAAG ACATTGAGGT GAAGAACCTA
    AAGCCGGCCA CGGTGAAGGT CTATGATTAT TATGAGACAG CGGAACATGC AGTTACCGAG
    TACAACTCCC CCTGCAGCTC GGATAAAGAA GCAGAAAACA CAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809689.1
    CDS130..4377
    Translation

    Target ORF information:

    RefSeq Version XM_012954235.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis alpha-2-macroglobulin-like 1 (a2ml1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012954235.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGTGCTGC TCAGGGCCCT GGGCTTGGCG CTGGTCCTAC TGCATCTCTC TGGGACATTG 
    GAGGCAAAGC TGCATTATGC TGTGGTCTTC CCCTCGGAGA TGCGCTGCCA TAGCTCCGAA
    CAAGCCTGTG TGCACCTCAG TGGGGCGGAG GGAGACAGCC GGATACAGGT TACCTTGAAC
    ATGGCCGAAC GGAACTCAAC GGTGATAGAG AGGAACCTCC GGCAGAAATC CCTCTTCAGC
    TGTGTTACAT TCCAGGTGCC TCCGCCCTCT GAAGGGAATG AAGAAGTGGC CACCATGGAA
    ATTCTCATTG AGAGCGCAGG GGAGACCATA ACCAACAGCA GCAAAGTTCT AGTGAAGAGG
    AGAAGAACCA GCACTTTCAT TCAGACGGAC AAAGCTGTAT ATAAACCAGG GCAAACAGTA
    CGATTCCGAG TGGTTTCTCT TAAAGAAAAT CTACAACCGG GAGAAAATCA GATTTCAGTC
    ATCCAATTAC AGGATCCAAA CAAGAACCGT ATTGCCCAGT GGCTGGATGT GACCCTCACA
    CAGGGAATCG CTGAGCTCTC ACTGCCGCTG TCCCCGGAAC CTTCGCTTGG AGAATATTCC
    ATTCACGTGG GGGACACTGT GCACTACTTT AGCGTGGAAG AATACGTCCT TCCCAAGTTT
    GAGGTCAGTT TCCAGTTCCC CAAGGTGGTG TTCTTTACCA GCGAACAATT CCAGCTGAAA
    ACATGTGGCA GATACACCTA TGGCAAACCT GTGCAAGGAA CCTATAAGGC TACGGTGTGC
    CGTAAGGTCA TAAGATACTG GTGGGGACAC AATGATGACC GCTCAGACCT GTGCGTCGAT
    TTCAGCGGGA AGTTGGACCA GTCTGGCTGT TCCACATTGA CAGCGACGTC TGAAAACTTC
    AATCTTACCT ACAACACTCA TGAAATGGGC CTAGAGGGGA CGTCTACTAT TACAGAGGGA
    GGGACAGGAG TGGAATATTC TGGAAGCGGT AAAACCATCA TTTCGTCTGT AGTCACGAGT
    TGCAGTTTCC TGGATGCTGA CAGTAACTAC AAGCCGGGGA TCCCATACAG CGGCACAGTA
    AAGGTGGTAG ATGCCAATGA TGACCCCATC CCCAACCAGA TAGTCTATCT AACCAGCCAT
    TCTAATGAAG TAAACAAGAC ATTGGTGTCG GATGAGAATG GACTCGCCTC CTTCCAGCTG
    GAGAACACGG CAGAGTGGAA GGGACAGCTG AGCCTTAGGG CCAGTACAAC TACACAGGAC
    GCTGGATTTG ATTTCCTTCA GGGCCGTCTC ATTACCCCTA ATGATGTTGC TTCTAAAGGG
    TCAGTAAAGG ACTCGGGATC AGTGGAAGTG CCCATTACTA GTGAAGGAGG AGGATCTTCA
    GAGCTGAGGG GTGAGGTCTC CATCAAACTC ACACTCAGTG CCGACGTCTC TCCGACTCTC
    CGCGCTCTCG CCTTCATTTC CCTCCCTAAT GGGGAATTAG TGGCCGATTC TGCAACATTC
    AAACTGCAAA GGTGCTTCAA GAACAAAGTA TCAGTTGGCT TCTCCCCAGA TGAAGTTCTC
    CCAGGAGCTG ATGTTTCCCT ACAAGTCCAG GCAGCCGCCG GGTCCCTGTG TGGCCTGAGA
    GTGGTGGATC AGAGTGTGGT GTTAATGAAA CCTGACAAAG AACTGACGGC CGATCAGATT
    CACAATCTCT TTCCTTTCCC TGATTTCGGA GGGTACGATG CTCGGGTCCA AGATAGTGAT
    GATCATTGTC CATTTTATCC CTGGTTTCAT CGACCAATGT GGAAGAGAAG TATTGTTCTA
    CCACCGTGGC ATATGCCTCA TGAAGATGAG GTTGATGTAT ACAGCTTATT TAAGGACATG
    CGCCTTAAGA TCGTCACTAG TGCGGAGATT AAGAAGCCTC TGAGGTGCCG GGAACCTGAA
    TTTGCTCTCC AGTACAACGT GCTCGCGCAC CCTCGAATTG CTGATGCTGG AGATGGTCTT
    GTAAGGGCAA TGCCGAGAGC AGTGGGAGTG GGAAATGAAC TCACTGAACC AGCGGCTGAA
    ATAATCCGGG CGTATTTCCC AGAGACGTGG ATCTGGCAGC TAGAGCCTGT GGGTGAGTCG
    GGTAGAGCCG AGGTCCATGG CTCAGCCCCA GACACCATCA CCGAGTGGAA TGCAGGAGCT
    TTCTGTATGG GACCCAGTGG GTTTGGCATC TCCCCACCAA CCTCCCTTCG AGCCTTCCAG
    CCCTTCTTTG TGGAGCTCAC TCTGCCGTAC TCTGTGGTGC GAGGGGAGTC ATTCACTCTC
    AAGGCCTCTG TGTTTAACTA CCTCAAGCAG TGTATAAAGG TTCAGACTAC ACTCCAGCCG
    TCACTTGAGT TAGAGCAGGA ACCATGTGCT GACTGTCAGT ATAGCAGCTG TATCTGTGCA
    GACGAAAGCA AGACCTTCTA CTGGAACCTT AAAGCCTCCA ACCTGGGAGA GGTGAACATC
    ACGGTGAGGA CGGAGGCTCT GAACACTCAG GACATGTGCA ATAATGAGAT TCCTATTGTT
    CCCAAGCAGG GTCACTCCGA CACTGTGATT AAGCCACTGC TGGTGCAGCC GGGGGGAGTC
    CTAATAGAGA AATCTCACAG CTCCCTGATC TGCAGCCCAG AGGGAGAAGA CCATTCAAAG
    ACAGAGGACA TATCCCTGAA TGTTCCAGAG AACATCCTGG AGGGTTCTGA GAGAGCGTAT
    GTAACAGCAC TGGGAGACCT GATGGGAACA GCCATGCAGA ACCTGGACCG GCTCCTGGCC
    ATGCCATATG GATGTGGGGA GCAGAACATG GTTCTCTTTG CCCCCAATAT CTTCATTCTT
    CAGTATCTGG AAAACACTCA CCAACTGAGC CCTGAGATCC AGACCAAAGC CAAAGGATTC
    CTTGAAAGCG GGTACCAAAG ACAACTGACT TACAAACGTA ACGATGGGTC CTACAGCGCA
    TTTGGGTCAA GGGACCCCGA GGGAAACACA TGGCTGACTG CATTTGTAGT GAAGTCCTTC
    AGCAAGGCTC GTCCCTACAT CTTCATCGAC GGAGACCACC TAAACCATTC TTTCTCATGG
    CTAAAAGATA AACGCCAAAG GAGCGGCTGC TTCCGTAGTG TGGGGAAACT CTTCAATAAT
    GCAATGAAGG GGGGAGTGGA TGATGAAATA TCTCTTTCTG CTTATGTCAC CATTGCTCTA
    ATGGAAGCCG GTCTGTCCAA AGAGGACCCA ATGGTTAGTG ATGCTTTATC CTGCCTGAGG
    AAGGCTGCCA GAAAAGTGAG CAGTGCCTAC ACCCAGGCTC TGCTGGCCTA CACTTTCACT
    CTGAGTGATG ATACTAAACA CAGGGAGAAG CTGCTGGCTA AACTGGAGGA GAAAGCTGTG
    AGGAAAGATG GACAACTCCA CTGGGAGAGG AAACCTGCCT CTAAGGCCTC TGACCTCCCA
    TACTGGTACC GCGCTCCCTC AGCCGAGGTG GAGTTAACCT CCTATGCGCT GCTGGCCCTT
    CTCTCTGGCC CCAACAAGGA CCTGGGCAAA GCTTCTGAGA TAGTCAACTG GCTGAGCAAG
    CAGCAAAATC CTTATGGGGG GTTCTCTTCT ACTCAGGACA CAGTTGTAGC TCTACAGGCT
    CTTGCTAAGT ATGCCGAGGC CACATTCTCT GACAAGGGAG ATGTCACAGT CACAGTCACT
    TCCAAGACTG GGTTCCATCA ACAGTTCCAT GTGGATCAGA CTAACCGGCT CCTGCTACAG
    AAAGCCTCAC TGCCAGATAT CCCTGGGGAG TACTCTCTGT CAGCCACTGG GAGCAGCTGT
    GTGTATGTAC AGACTGTCCT GAGATACAAC ATTCCCCCAC CTAGAAGTGA CGCCACCTTC
    TCAGTACGAG TGGAGACCCA ACCAAAGCAA TGTCCCCAGG AGCCAATGAA ACAGATGCAC
    ATAGACATTT ACATTCAATA CACAGGGTCT CGGGAGAAAT CCAACATGGC TCTGGTTGAG
    GTCAAGATGC TCTCAGGCTT TATCCCCGTG AAGAGCACAA TAAAGCAGCT AGTGAGCAGC
    AATACGATAA AGCGGAGTGA GATCCAAACT GATATGGTGA CTTTGTACCT GAATGAGTTA
    GGACATGATC CAGTTCACTT CTCGATTATG GTGGAACAAG ACATTGAGGT GAAGAACCTA
    AAGCCGGCCA CGGTGAAGGT CTATGATTAT TATGAGACAG CGGAACATGC AGTTACCGAG
    TACAACTCCC CCTGCAGCTC GGATAAAGAA GCAGAAAACA CAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.