A2ML1 cDNA ORF clone, Bos taurus(cattle)

The following A2ML1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A2ML1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36018 NM_001191301.1
Latest version!
Bos taurus alpha-2-macroglobulin like 1 (A2ML1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa19735 XM_005207059.3
Latest version!
Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49589 XM_010805531.2
Latest version!
Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49590 XM_010805532.2
Latest version!
Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49591 XM_010805533.2
Latest version!
Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa49589 XM_010805531.1 Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa19735 XM_005207059.2 Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49590 XM_010805532.1 Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa49591 XM_010805533.1 Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36018
    Clone ID Related Accession (Same CDS sequence) NM_001191301.1
    Accession Version NM_001191301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-24
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On Jul 17, 2010 this sequence version replaced XM_001788335.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145467 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGTGAATGAA GCTCGTCCAA TGGCTTTCAA GCCAGCTCCA
    TTCCAGCCAG TTCAGCCGGA AGAGTCTCAG GTTCGCCAGT ACTTTCCAGA GACTTGGCTG
    TGGGATCTGT TTCCTGTTGG CTACTCGGGA GAGGAGTCTG TCCACATCAC GGTTCCTGAC
    ACCATCACCA CGTGGAAGGC CATGACTTTC TGCACCTCCC AGTCCAAGGG TTTTGGCCTC
    TCACCCACCG TTGGAGTGAC TGCCTTCAAG CCGTTCTTTG TTGATCTGAC GCTCCCTTAC
    TCAGTGGTTC GTGGTGAATC CTTCCGTCTG ACTGCCACCA TCTTCAATTA CCTGAAGAAG
    TGCATCAGGG TTCAGACTAA CCTAGCTAAG TCAGAGGAGT ACCGAGTGGA AACGCAGACA
    GCCACTCGGG CCTCCACCTG TCTGTGTGCT GACGAAGCAA AATCCTATCA CTGGAACATC
    ACGGCTGTCA AACTGGGTCA TGTAAACTTC ACTATCAGCA CAAAGATTCT GAACAGCAAT
    GAACTCTGTG GGGGCCAGAA GGCGTTTGTT CCCCTAAAGG GTGGGAGTGA CACACTCATC
    AAACCAGTTC TGGTCAAGCC GGAAGGAGTC CTTGTGGAGA AGACACACAG CTCACTGCTG
    TGCCCAAAAG GAAAAGTGGC TTCAGAATCT ATCTCCCTGG AGGTCCCTGT GGATGTTGTT
    CCTGATTCAG CCAGGGCTTA TGTTGCTGTT CTGGGAGACA TCATGGGCAC AGCCCTGCAG
    AACCTGGACA ACTTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTC
    GCACCTATCA TTTACGTCCT GCAGTACCTG GAGAAGACAG AGCTGCTGAC GGAGGAAATC
    TGGTCTCGAG CAGTGGGCTT CCTGAAGCTG GGGTACCAGA GGGAACTGAT GTACAAACAC
    AGTAATGGCT CCTACAGTGC CTTTGGGGAG CAAGATGGAA ATGGAAACAC ATGGCTGACA
    GCCTTTGTCA CCAAATGCTT TGGCCAGGCT CGGCAATTCA TCTTCATTGA CGACAAGAAC
    ATCCAGGATG CTCTTGAGTG GATGGCAGGA AACCAGCTCT CCAGTGGCTG CTACGACAAC
    GTGGGAAAGC TCCTTCAAAC ATCTATGAAG GGTGGAGTTG ATGATGAGGT CTCCTTGACT
    GCCTACATTA CAGCTGCATT GTTGGAAATG GGCAAGACCA TAGATGACCC AATGGTGAGT
    CAGGGGCTGC AGTGTGTCAA GAGTTCTGTT TCCTCTACCA CCAACCTGTA CACACAGGCC
    CTCCTTGCTT ATACGTTCTC TCTGGCTGGA AACATGAACA TCAGAAACAT TCTTCTTGAA
    AAGCTAGATC AACAGGCTAT TGTCTCAGCA GCAAAATGCA TATGGGGCTT CTCTTCCACT
    CAGGACACCG TGGTGGCTCT CCAAGCTCTC GCCAAGTATG CCACCACTGC CTACGTGCCG
    TCGGAGCAAG TCAACCTGGC AGTGAAATCC GCTCAGAATT TCCAGCACAC CTTCAATGTT
    CAAGCTGCCA ACCGTCTGGT GTTCCAGCAG GAGATGTTGC CCCATATCCC TGGGGTGTAC
    ACCTTGGAGG CCTCAGGCAA GGGCTGTATT TATGCGCAGA CGGTGCTGAG ATATAATATC
    CTCCCTCCGA AAAGTCTGGA GGCCTTTAGT CTTAGTGTGA AAACAACGGG ATCTCAGTGT
    AAGCAACATA CTTCACTTGG ATCCTTGGTG CTGACGATCC AAACCAGTTA TGTGGGGAGT
    CGCAGCTCTT CCAATATGGC TATTGTGGAG GTCAAGATGC TTTCTGGATT CAGTCCTGTG
    GAGGGCACCA ATCAGTTACT TCTCCAGCAA CCACTGGTGA AGAAGGTTGA ATCTGAAATT
    GACACACTCA ACATCTACTT GGAAGAGCTC GGGAAGGAAA CTCAGACCTA TACCTTCACC
    ATCAGCCAAA GTGTGTTGGT CACCAACCTC AAACCAGCAA CCATCAAAGT CTATGACTAC
    TACCTGCCAG ATGAACAGGC AACAATTCAG TATTCTGATT CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178230.1
    CDS1..4191
    Translation

    Target ORF information:

    RefSeq Version NM_001191301.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin like 1 (A2ML1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGTGAATGAA GCTCGTCCAA TGGCTTTCAA GCCAGCTCCA
    TTCCAGCCAG TTCAGCCGGA AGAGTCTCAG GTTCGCCAGT ACTTTCCAGA GACTTGGCTG
    TGGGATCTGT TTCCTGTTGG CTACTCGGGA GAGGAGTCTG TCCACATCAC GGTTCCTGAC
    ACCATCACCA CGTGGAAGGC CATGACTTTC TGCACCTCCC AGTCCAAGGG TTTTGGCCTC
    TCACCCACCG TTGGAGTGAC TGCCTTCAAG CCGTTCTTTG TTGATCTGAC GCTCCCTTAC
    TCAGTGGTTC GTGGTGAATC CTTCCGTCTG ACTGCCACCA TCTTCAATTA CCTGAAGAAG
    TGCATCAGGG TTCAGACTAA CCTAGCTAAG TCAGAGGAGT ACCGAGTGGA AACGCAGACA
    GCCACTCGGG CCTCCACCTG TCTGTGTGCT GACGAAGCAA AATCCTATCA CTGGAACATC
    ACGGCTGTCA AACTGGGTCA TGTAAACTTC ACTATCAGCA CAAAGATTCT GAACAGCAAT
    GAACTCTGTG GGGGCCAGAA GGCGTTTGTT CCCCTAAAGG GTGGGAGTGA CACACTCATC
    AAACCAGTTC TGGTCAAGCC GGAAGGAGTC CTTGTGGAGA AGACACACAG CTCACTGCTG
    TGCCCAAAAG GAAAAGTGGC TTCAGAATCT ATCTCCCTGG AGGTCCCTGT GGATGTTGTT
    CCTGATTCAG CCAGGGCTTA TGTTGCTGTT CTGGGAGACA TCATGGGCAC AGCCCTGCAG
    AACCTGGACA ACTTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTC
    GCACCTATCA TTTACGTCCT GCAGTACCTG GAGAAGACAG AGCTGCTGAC GGAGGAAATC
    TGGTCTCGAG CAGTGGGCTT CCTGAAGCTG GGGTACCAGA GGGAACTGAT GTACAAACAC
    AGTAATGGCT CCTACAGTGC CTTTGGGGAG CAAGATGGAA ATGGAAACAC ATGGCTGACA
    GCCTTTGTCA CCAAATGCTT TGGCCAGGCT CGGCAATTCA TCTTCATTGA CGACAAGAAC
    ATCCAGGATG CTCTTGAGTG GATGGCAGGA AACCAGCTCT CCAGTGGCTG CTACGACAAC
    GTGGGAAAGC TCCTTCAAAC ATCTATGAAG GGTGGAGTTG ATGATGAGGT CTCCTTGACT
    GCCTACATTA CAGCTGCATT GTTGGAAATG GGCAAGACCA TAGATGACCC AATGGTGAGT
    CAGGGGCTGC AGTGTGTCAA GAGTTCTGTT TCCTCTACCA CCAACCTGTA CACACAGGCC
    CTCCTTGCTT ATACGTTCTC TCTGGCTGGA AACATGAACA TCAGAAACAT TCTTCTTGAA
    AAGCTAGATC AACAGGCTAT TGTCTCAGCA GCAAAATGCA TATGGGGCTT CTCTTCCACT
    CAGGACACCG TGGTGGCTCT CCAAGCTCTC GCCAAGTATG CCACCACTGC CTACGTGCCG
    TCGGAGCAAG TCAACCTGGC AGTGAAATCC GCTCAGAATT TCCAGCACAC CTTCAATGTT
    CAAGCTGCCA ACCGTCTGGT GTTCCAGCAG GAGATGTTGC CCCATATCCC TGGGGTGTAC
    ACCTTGGAGG CCTCAGGCAA GGGCTGTATT TATGCGCAGA CGGTGCTGAG ATATAATATC
    CTCCCTCCGA AAAGTCTGGA GGCCTTTAGT CTTAGTGTGA AAACAACGGG ATCTCAGTGT
    AAGCAACATA CTTCACTTGG ATCCTTGGTG CTGACGATCC AAACCAGTTA TGTGGGGAGT
    CGCAGCTCTT CCAATATGGC TATTGTGGAG GTCAAGATGC TTTCTGGATT CAGTCCTGTG
    GAGGGCACCA ATCAGTTACT TCTCCAGCAA CCACTGGTGA AGAAGGTTGA ATCTGAAATT
    GACACACTCA ACATCTACTT GGAAGAGCTC GGGAAGGAAA CTCAGACCTA TACCTTCACC
    ATCAGCCAAA GTGTGTTGGT CACCAACCTC AAACCAGCAA CCATCAAAGT CTATGACTAC
    TACCTGCCAG ATGAACAGGC AACAATTCAG TATTCTGATT CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19735
    Clone ID Related Accession (Same CDS sequence) XM_005207059.2 , XM_005207059.3
    Accession Version XM_005207059.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103939.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005207059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGTGAATGAA GCTCGTCCAA TGGCTTTCAA GCCAGCTCCA
    TTCCAGCCAG TTCAGCCGGA AGAGTCTCAG GTTCGCCAGT ACTTTCCAGA GACTTGGCTG
    TGGGATCTGT TTCCTGTTGG CTACTCGGGA GAGGAGTCTG TCCACATCAC GGTTCCTGAC
    ACCATCACCA CGTGGAAGGC CATGACTTTC TGCACCTCCC AGTCCAAGGG TTTTGGCCTC
    TCACCCACCG TTGGAGTGAC TGCCTTCAAG CCGTTCTTTG TTGATCTGAC GCTCCCTTAC
    TCAGTGGTTC GTGGTGAATC CTTCCGTCTG ACTGCCACCA TCTTCAATTA CCTGAAGAAG
    TGCATCAGGG TTCAGACTAA CCTAGCTAAG TCAGAGGAGT ACCGAGTGGA AACGCAGACA
    GCCACTCGGG CCTCCACCTG TCTGTGTGCT GACGAAGCAA AATCCTATCA CTGGAACATC
    ACGGCTGTCA AACTGGGTCA TGTAAACTTC ACTATCAGCA CAAAGATTCT GAACAGCAAT
    GAACTCTGTG GGGGCCAGAA GGCGTTTGTT CCCCTAAAGG GTGGGAGTGA CACACTCATC
    AAACCAGTTC TGGTCAAGCC GGAAGGAGTC CTTGTGGAGA AGACACACAG CTCACTGCTG
    TGCCCAAAAG GAAAAGTGGC TTCAGAATCT ATCTCCCTGG AGGTCCCTGT GGATGTTGTT
    CCTGATTCAG CCAGGGCTTA TGTTGCTGTT CTGGGAGACA TCATGGGCAC AGCCCTGCAG
    AACCTGGACA ACTTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTC
    GCACCTATCA TTTACGTCCT GCAGTACCTG GAGAAGACAG AGCTGCTGAC GGAGGAAATC
    TGGTCTCGAG CAGTGGGCTT CCTGAAGCTG GGGTACCAGA GGGAACTGAT GTACAAACAC
    AGTAATGGCT CCTACAGTGC CTTTGGGGAG CAAGATGGAA ATGGAAACAC ATGGCTGACA
    GCCTTTGTCA CCAAATGCTT TGGCCAGGCT CGGCAATTCA TCTTCATTGA CGACAAGAAC
    ATCCAGGATG CTCTTGAGTG GATGGCAGGA AACCAGCTCT CCAGTGGCTG CTACGACAAC
    GTGGGAAAGC TCCTTCAAAC ATCTATGAAG GGTGGAGTTG ATGATGAGGT CTCCTTGACT
    GCCTACATTA CAGCTGCATT GTTGGAAATG GGCAAGACCA TAGATGACCC AATGGTGAGT
    CAGGGGCTGC AGTGTGTCAA GAGTTCTGTT TCCTCTACCA CCAACCTGTA CACACAGGCC
    CTCCTTGCTT ATACGTTCTC TCTGGCTGGA AACATGAACA TCAGAAACAT TCTTCTTGAA
    AAGCTAGATC AACAGGCTAT TGTCTCAGGA GAGTCCACTC ACTGGAGCCA GAAACCTGCT
    CCGACTTCAG ATACCAGCCC TTGGTCTCAG CCTGAGGCTG TGGATGTGGA ACTCACGGCC
    TATGTATTAC TGACCTTGCT TAGCAAAGAT GACCTGACTC AAAAGGAGCT AGGCAAGGCC
    ACCAGCATAG TGGCTTGGTT GACCAAGCAG CAAAATGCAT ATGGGGGCTT CTCTTCCACT
    CAGGACACCG TGGTGGCTCT CCAAGCTCTC GCCAAGTATG CCACCACTGC CTACGTGCCG
    TCGGAGCAAG TCAACCTGGC AGTGAAATCC GCTCAGAATT TCCAGCACAC CTTCAATGTT
    CAAGCTGCCA ACCGTCTGGT GTTCCAGCAG GAGATGTTGC CCCATATCCC TGGGGTGTAC
    ACCTTGGAGG CCTCAGGCAA GGGCTGTATT TATGCGCAGA CGGTGCTGAG ATATAATATC
    CTCCCTCCGA AAAGTCTGGA GGCCTTTAGT CTTAGTGTGA AAACAACGGG ATCTCAGTGT
    AAGCAACATA CTTCACTTGG ATCCTTGGTG CTGACGATCC AAACCAGTTA TGTGGGGAGT
    CGCAGCTCTT CCAATATGGC TATTGTGGAG GTCAAGATGC TTTCTGGATT CAGTCCTGTG
    GAGGGCACCA ATCAGTTACT TCTCCAGCAA CCACTGGTGA AGAAGGTTGA ATCTGAAATT
    GACACACTCA ACATCTACTT GGAAGAGCTC GGGAAGGAAA CTCAGACCTA TACCTTCACC
    ATCAGCCAAA GTGTGTTGGT CACCAACCTC AAACCAGCAA CCATCAAAGT CTATGACTAC
    TACCTGCCAG ATGAACAGGC AACAATTCAG TATTCTGATT CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207116.1
    CDS40..4410
    Translation

    Target ORF information:

    RefSeq Version XM_005207059.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207059.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGTGAATGAA GCTCGTCCAA TGGCTTTCAA GCCAGCTCCA
    TTCCAGCCAG TTCAGCCGGA AGAGTCTCAG GTTCGCCAGT ACTTTCCAGA GACTTGGCTG
    TGGGATCTGT TTCCTGTTGG CTACTCGGGA GAGGAGTCTG TCCACATCAC GGTTCCTGAC
    ACCATCACCA CGTGGAAGGC CATGACTTTC TGCACCTCCC AGTCCAAGGG TTTTGGCCTC
    TCACCCACCG TTGGAGTGAC TGCCTTCAAG CCGTTCTTTG TTGATCTGAC GCTCCCTTAC
    TCAGTGGTTC GTGGTGAATC CTTCCGTCTG ACTGCCACCA TCTTCAATTA CCTGAAGAAG
    TGCATCAGGG TTCAGACTAA CCTAGCTAAG TCAGAGGAGT ACCGAGTGGA AACGCAGACA
    GCCACTCGGG CCTCCACCTG TCTGTGTGCT GACGAAGCAA AATCCTATCA CTGGAACATC
    ACGGCTGTCA AACTGGGTCA TGTAAACTTC ACTATCAGCA CAAAGATTCT GAACAGCAAT
    GAACTCTGTG GGGGCCAGAA GGCGTTTGTT CCCCTAAAGG GTGGGAGTGA CACACTCATC
    AAACCAGTTC TGGTCAAGCC GGAAGGAGTC CTTGTGGAGA AGACACACAG CTCACTGCTG
    TGCCCAAAAG GAAAAGTGGC TTCAGAATCT ATCTCCCTGG AGGTCCCTGT GGATGTTGTT
    CCTGATTCAG CCAGGGCTTA TGTTGCTGTT CTGGGAGACA TCATGGGCAC AGCCCTGCAG
    AACCTGGACA ACTTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTC
    GCACCTATCA TTTACGTCCT GCAGTACCTG GAGAAGACAG AGCTGCTGAC GGAGGAAATC
    TGGTCTCGAG CAGTGGGCTT CCTGAAGCTG GGGTACCAGA GGGAACTGAT GTACAAACAC
    AGTAATGGCT CCTACAGTGC CTTTGGGGAG CAAGATGGAA ATGGAAACAC ATGGCTGACA
    GCCTTTGTCA CCAAATGCTT TGGCCAGGCT CGGCAATTCA TCTTCATTGA CGACAAGAAC
    ATCCAGGATG CTCTTGAGTG GATGGCAGGA AACCAGCTCT CCAGTGGCTG CTACGACAAC
    GTGGGAAAGC TCCTTCAAAC ATCTATGAAG GGTGGAGTTG ATGATGAGGT CTCCTTGACT
    GCCTACATTA CAGCTGCATT GTTGGAAATG GGCAAGACCA TAGATGACCC AATGGTGAGT
    CAGGGGCTGC AGTGTGTCAA GAGTTCTGTT TCCTCTACCA CCAACCTGTA CACACAGGCC
    CTCCTTGCTT ATACGTTCTC TCTGGCTGGA AACATGAACA TCAGAAACAT TCTTCTTGAA
    AAGCTAGATC AACAGGCTAT TGTCTCAGGA GAGTCCACTC ACTGGAGCCA GAAACCTGCT
    CCGACTTCAG ATACCAGCCC TTGGTCTCAG CCTGAGGCTG TGGATGTGGA ACTCACGGCC
    TATGTATTAC TGACCTTGCT TAGCAAAGAT GACCTGACTC AAAAGGAGCT AGGCAAGGCC
    ACCAGCATAG TGGCTTGGTT GACCAAGCAG CAAAATGCAT ATGGGGGCTT CTCTTCCACT
    CAGGACACCG TGGTGGCTCT CCAAGCTCTC GCCAAGTATG CCACCACTGC CTACGTGCCG
    TCGGAGCAAG TCAACCTGGC AGTGAAATCC GCTCAGAATT TCCAGCACAC CTTCAATGTT
    CAAGCTGCCA ACCGTCTGGT GTTCCAGCAG GAGATGTTGC CCCATATCCC TGGGGTGTAC
    ACCTTGGAGG CCTCAGGCAA GGGCTGTATT TATGCGCAGA CGGTGCTGAG ATATAATATC
    CTCCCTCCGA AAAGTCTGGA GGCCTTTAGT CTTAGTGTGA AAACAACGGG ATCTCAGTGT
    AAGCAACATA CTTCACTTGG ATCCTTGGTG CTGACGATCC AAACCAGTTA TGTGGGGAGT
    CGCAGCTCTT CCAATATGGC TATTGTGGAG GTCAAGATGC TTTCTGGATT CAGTCCTGTG
    GAGGGCACCA ATCAGTTACT TCTCCAGCAA CCACTGGTGA AGAAGGTTGA ATCTGAAATT
    GACACACTCA ACATCTACTT GGAAGAGCTC GGGAAGGAAA CTCAGACCTA TACCTTCACC
    ATCAGCCAAA GTGTGTTGGT CACCAACCTC AAACCAGCAA CCATCAAAGT CTATGACTAC
    TACCTGCCAG ATGAACAGGC AACAATTCAG TATTCTGATT CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19735
    Clone ID Related Accession (Same CDS sequence) XM_005207059.2 , XM_005207059.3
    Accession Version XM_005207059.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGTGAATGAA GCTCGTCCAA TGGCTTTCAA GCCAGCTCCA
    TTCCAGCCAG TTCAGCCGGA AGAGTCTCAG GTTCGCCAGT ACTTTCCAGA GACTTGGCTG
    TGGGATCTGT TTCCTGTTGG CTACTCGGGA GAGGAGTCTG TCCACATCAC GGTTCCTGAC
    ACCATCACCA CGTGGAAGGC CATGACTTTC TGCACCTCCC AGTCCAAGGG TTTTGGCCTC
    TCACCCACCG TTGGAGTGAC TGCCTTCAAG CCGTTCTTTG TTGATCTGAC GCTCCCTTAC
    TCAGTGGTTC GTGGTGAATC CTTCCGTCTG ACTGCCACCA TCTTCAATTA CCTGAAGAAG
    TGCATCAGGG TTCAGACTAA CCTAGCTAAG TCAGAGGAGT ACCGAGTGGA AACGCAGACA
    GCCACTCGGG CCTCCACCTG TCTGTGTGCT GACGAAGCAA AATCCTATCA CTGGAACATC
    ACGGCTGTCA AACTGGGTCA TGTAAACTTC ACTATCAGCA CAAAGATTCT GAACAGCAAT
    GAACTCTGTG GGGGCCAGAA GGCGTTTGTT CCCCTAAAGG GTGGGAGTGA CACACTCATC
    AAACCAGTTC TGGTCAAGCC GGAAGGAGTC CTTGTGGAGA AGACACACAG CTCACTGCTG
    TGCCCAAAAG GAAAAGTGGC TTCAGAATCT ATCTCCCTGG AGGTCCCTGT GGATGTTGTT
    CCTGATTCAG CCAGGGCTTA TGTTGCTGTT CTGGGAGACA TCATGGGCAC AGCCCTGCAG
    AACCTGGACA ACTTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTC
    GCACCTATCA TTTACGTCCT GCAGTACCTG GAGAAGACAG AGCTGCTGAC GGAGGAAATC
    TGGTCTCGAG CAGTGGGCTT CCTGAAGCTG GGGTACCAGA GGGAACTGAT GTACAAACAC
    AGTAATGGCT CCTACAGTGC CTTTGGGGAG CAAGATGGAA ATGGAAACAC ATGGCTGACA
    GCCTTTGTCA CCAAATGCTT TGGCCAGGCT CGGCAATTCA TCTTCATTGA CGACAAGAAC
    ATCCAGGATG CTCTTGAGTG GATGGCAGGA AACCAGCTCT CCAGTGGCTG CTACGACAAC
    GTGGGAAAGC TCCTTCAAAC ATCTATGAAG GGTGGAGTTG ATGATGAGGT CTCCTTGACT
    GCCTACATTA CAGCTGCATT GTTGGAAATG GGCAAGACCA TAGATGACCC AATGGTGAGT
    CAGGGGCTGC AGTGTGTCAA GAGTTCTGTT TCCTCTACCA CCAACCTGTA CACACAGGCC
    CTCCTTGCTT ATACGTTCTC TCTGGCTGGA AACATGAACA TCAGAAACAT TCTTCTTGAA
    AAGCTAGATC AACAGGCTAT TGTCTCAGGA GAGTCCACTC ACTGGAGCCA GAAACCTGCT
    CCGACTTCAG ATACCAGCCC TTGGTCTCAG CCTGAGGCTG TGGATGTGGA ACTCACGGCC
    TATGTATTAC TGACCTTGCT TAGCAAAGAT GACCTGACTC AAAAGGAGCT AGGCAAGGCC
    ACCAGCATAG TGGCTTGGTT GACCAAGCAG CAAAATGCAT ATGGGGGCTT CTCTTCCACT
    CAGGACACCG TGGTGGCTCT CCAAGCTCTC GCCAAGTATG CCACCACTGC CTACGTGCCG
    TCGGAGCAAG TCAACCTGGC AGTGAAATCC GCTCAGAATT TCCAGCACAC CTTCAATGTT
    CAAGCTGCCA ACCGTCTGGT GTTCCAGCAG GAGATGTTGC CCCATATCCC TGGGGTGTAC
    ACCTTGGAGG CCTCAGGCAA GGGCTGTATT TATGCGCAGA CGGTGCTGAG ATATAATATC
    CTCCCTCCGA AAAGTCTGGA GGCCTTTAGT CTTAGTGTGA AAACAACGGG ATCTCAGTGT
    AAGCAACATA CTTCACTTGG ATCCTTGGTG CTGACGATCC AAACCAGTTA TGTGGGGAGT
    CGCAGCTCTT CCAATATGGC TATTGTGGAG GTCAAGATGC TTTCTGGATT CAGTCCTGTG
    GAGGGCACCA ATCAGTTACT TCTCCAGCAA CCACTGGTGA AGAAGGTTGA ATCTGAAATT
    GACACACTCA ACATCTACTT GGAAGAGCTC GGGAAGGAAA CTCAGACCTA TACCTTCACC
    ATCAGCCAAA GTGTGTTGGT CACCAACCTC AAACCAGCAA CCATCAAAGT CTATGACTAC
    TACCTGCCAG ATGAACAGGC AACAATTCAG TATTCTGATT CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207116.1
    CDS54..4424
    Translation

    Target ORF information:

    RefSeq Version XM_005207059.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207059.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGTGAATGAA GCTCGTCCAA TGGCTTTCAA GCCAGCTCCA
    TTCCAGCCAG TTCAGCCGGA AGAGTCTCAG GTTCGCCAGT ACTTTCCAGA GACTTGGCTG
    TGGGATCTGT TTCCTGTTGG CTACTCGGGA GAGGAGTCTG TCCACATCAC GGTTCCTGAC
    ACCATCACCA CGTGGAAGGC CATGACTTTC TGCACCTCCC AGTCCAAGGG TTTTGGCCTC
    TCACCCACCG TTGGAGTGAC TGCCTTCAAG CCGTTCTTTG TTGATCTGAC GCTCCCTTAC
    TCAGTGGTTC GTGGTGAATC CTTCCGTCTG ACTGCCACCA TCTTCAATTA CCTGAAGAAG
    TGCATCAGGG TTCAGACTAA CCTAGCTAAG TCAGAGGAGT ACCGAGTGGA AACGCAGACA
    GCCACTCGGG CCTCCACCTG TCTGTGTGCT GACGAAGCAA AATCCTATCA CTGGAACATC
    ACGGCTGTCA AACTGGGTCA TGTAAACTTC ACTATCAGCA CAAAGATTCT GAACAGCAAT
    GAACTCTGTG GGGGCCAGAA GGCGTTTGTT CCCCTAAAGG GTGGGAGTGA CACACTCATC
    AAACCAGTTC TGGTCAAGCC GGAAGGAGTC CTTGTGGAGA AGACACACAG CTCACTGCTG
    TGCCCAAAAG GAAAAGTGGC TTCAGAATCT ATCTCCCTGG AGGTCCCTGT GGATGTTGTT
    CCTGATTCAG CCAGGGCTTA TGTTGCTGTT CTGGGAGACA TCATGGGCAC AGCCCTGCAG
    AACCTGGACA ACTTGGTGCA GATGCCAAGG GGCTGTGGGG AGCAGAACAT GGTCTTGTTC
    GCACCTATCA TTTACGTCCT GCAGTACCTG GAGAAGACAG AGCTGCTGAC GGAGGAAATC
    TGGTCTCGAG CAGTGGGCTT CCTGAAGCTG GGGTACCAGA GGGAACTGAT GTACAAACAC
    AGTAATGGCT CCTACAGTGC CTTTGGGGAG CAAGATGGAA ATGGAAACAC ATGGCTGACA
    GCCTTTGTCA CCAAATGCTT TGGCCAGGCT CGGCAATTCA TCTTCATTGA CGACAAGAAC
    ATCCAGGATG CTCTTGAGTG GATGGCAGGA AACCAGCTCT CCAGTGGCTG CTACGACAAC
    GTGGGAAAGC TCCTTCAAAC ATCTATGAAG GGTGGAGTTG ATGATGAGGT CTCCTTGACT
    GCCTACATTA CAGCTGCATT GTTGGAAATG GGCAAGACCA TAGATGACCC AATGGTGAGT
    CAGGGGCTGC AGTGTGTCAA GAGTTCTGTT TCCTCTACCA CCAACCTGTA CACACAGGCC
    CTCCTTGCTT ATACGTTCTC TCTGGCTGGA AACATGAACA TCAGAAACAT TCTTCTTGAA
    AAGCTAGATC AACAGGCTAT TGTCTCAGGA GAGTCCACTC ACTGGAGCCA GAAACCTGCT
    CCGACTTCAG ATACCAGCCC TTGGTCTCAG CCTGAGGCTG TGGATGTGGA ACTCACGGCC
    TATGTATTAC TGACCTTGCT TAGCAAAGAT GACCTGACTC AAAAGGAGCT AGGCAAGGCC
    ACCAGCATAG TGGCTTGGTT GACCAAGCAG CAAAATGCAT ATGGGGGCTT CTCTTCCACT
    CAGGACACCG TGGTGGCTCT CCAAGCTCTC GCCAAGTATG CCACCACTGC CTACGTGCCG
    TCGGAGCAAG TCAACCTGGC AGTGAAATCC GCTCAGAATT TCCAGCACAC CTTCAATGTT
    CAAGCTGCCA ACCGTCTGGT GTTCCAGCAG GAGATGTTGC CCCATATCCC TGGGGTGTAC
    ACCTTGGAGG CCTCAGGCAA GGGCTGTATT TATGCGCAGA CGGTGCTGAG ATATAATATC
    CTCCCTCCGA AAAGTCTGGA GGCCTTTAGT CTTAGTGTGA AAACAACGGG ATCTCAGTGT
    AAGCAACATA CTTCACTTGG ATCCTTGGTG CTGACGATCC AAACCAGTTA TGTGGGGAGT
    CGCAGCTCTT CCAATATGGC TATTGTGGAG GTCAAGATGC TTTCTGGATT CAGTCCTGTG
    GAGGGCACCA ATCAGTTACT TCTCCAGCAA CCACTGGTGA AGAAGGTTGA ATCTGAAATT
    GACACACTCA ACATCTACTT GGAAGAGCTC GGGAAGGAAA CTCAGACCTA TACCTTCACC
    ATCAGCCAAA GTGTGTTGGT CACCAACCTC AAACCAGCAA CCATCAAAGT CTATGACTAC
    TACCTGCCAG ATGAACAGGC AACAATTCAG TATTCTGATT CCTGTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49589
    Clone ID Related Accession (Same CDS sequence) XM_010805531.1 , XM_010805531.2
    Accession Version XM_010805531.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103939.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGCAGTG AATGAAGCTC GTCCAATGGC TTTCAAGCCA
    GCTCCATTCC AGCCAGTTCA GCCGGAAGAG TCTCAGGTTC GCCAGTACTT TCCAGAGACT
    TGGCTGTGGG ATCTGTTTCC TGTTGGCTAC TCGGGAGAGG AGTCTGTCCA CATCACGGTT
    CCTGACACCA TCACCACGTG GAAGGCCATG ACTTTCTGCA CCTCCCAGTC CAAGGGTTTT
    GGCCTCTCAC CCACCGTTGG AGTGACTGCC TTCAAGCCGT TCTTTGTTGA TCTGACGCTC
    CCTTACTCAG TGGTTCGTGG TGAATCCTTC CGTCTGACTG CCACCATCTT CAATTACCTG
    AAGAAGTGCA TCAGGGTTCA GACTAACCTA GCTAAGTCAG AGGAGTACCG AGTGGAAACG
    CAGACAGCCA CTCGGGCCTC CACCTGTCTG TGTGCTGACG AAGCAAAATC CTATCACTGG
    AACATCACGG CTGTCAAACT GGGTCATGTA AACTTCACTA TCAGCACAAA GATTCTGAAC
    AGCAATGAAC TCTGTGGGGG CCAGAAGGCG TTTGTTCCCC TAAAGGGTGG GAGTGACACA
    CTCATCAAAC CAGTTCTGGT CAAGCCGGAA GGAGTCCTTG TGGAGAAGAC ACACAGCTCA
    CTGCTGTGCC CAAAAGGAAA AGTGGCTTCA GAATCTATCT CCCTGGAGGT CCCTGTGGAT
    GTTGTTCCTG ATTCAGCCAG GGCTTATGTT GCTGTTCTGG GAGACATCAT GGGCACAGCC
    CTGCAGAACC TGGACAACTT GGTGCAGATG CCAAGGGGCT GTGGGGAGCA GAACATGGTC
    TTGTTCGCAC CTATCATTTA CGTCCTGCAG TACCTGGAGA AGACAGAGCT GCTGACGGAG
    GAAATCTGGT CTCGAGCAGT GGGCTTCCTG AAGCTGGGGT ACCAGAGGGA ACTGATGTAC
    AAACACAGTA ATGGCTCCTA CAGTGCCTTT GGGGAGCAAG ATGGAAATGG AAACACATGG
    CTGACAGCCT TTGTCACCAA ATGCTTTGGC CAGGCTCGGC AATTCATCTT CATTGACGAC
    AAGAACATCC AGGATGCTCT TGAGTGGATG GCAGGAAACC AGCTCTCCAG TGGCTGCTAC
    GACAACGTGG GAAAGCTCCT TCAAACATCT ATGAAGGGTG GAGTTGATGA TGAGGTCTCC
    TTGACTGCCT ACATTACAGC TGCATTGTTG GAAATGGGCA AGACCATAGA TGACCCAATG
    GTGAGTCAGG GGCTGCAGTG TGTCAAGAGT TCTGTTTCCT CTACCACCAA CCTGTACACA
    CAGGCCCTCC TTGCTTATAC GTTCTCTCTG GCTGGAAACA TGAACATCAG AAACATTCTT
    CTTGAAAAGC TAGATCAACA GGCTATTGTC TCAGGAGAGT CCACTCACTG GAGCCAGAAA
    CCTGCTCCGA CTTCAGATAC CAGCCCTTGG TCTCAGCCTG AGGCTGTGGA TGTGGAACTC
    ACGGCCTATG TATTACTGAC CTTGCTTAGC AAAGATGACC TGACTCAAAA GGAGCTAGGC
    AAGGCCACCA GCATAGTGGC TTGGTTGACC AAGCAGCAAA ATGCATATGG GGGCTTCTCT
    TCCACTCAGG ACACCGTGGT GGCTCTCCAA GCTCTCGCCA AGTATGCCAC CACTGCCTAC
    GTGCCGTCGG AGCAAGTCAA CCTGGCAGTG AAATCCGCTC AGAATTTCCA GCACACCTTC
    AATGTTCAAG CTGCCAACCG TCTGGTGTTC CAGCAGGAGA TGTTGCCCCA TATCCCTGGG
    GTGTACACCT TGGAGGCCTC AGGCAAGGGC TGTATTTATG CGCAGACGGT GCTGAGATAT
    AATATCCTCC CTCCGAAAAG TCTGGAGGCC TTTAGTCTTA GTGTGAAAAC AACGGGATCT
    CAGTGTAAGC AACATACTTC ACTTGGATCC TTGGTGCTGA CGATCCAAAC CAGTTATGTG
    GGGAGTCGCA GCTCTTCCAA TATGGCTATT GTGGAGGTCA AGATGCTTTC TGGATTCAGT
    CCTGTGGAGG GCACCAATCA GTTACTTCTC CAGCAACCAC TGGTGAAGAA GGTTGAATCT
    GAAATTGACA CACTCAACAT CTACTTGGAA GAGCTCGGGA AGGAAACTCA GACCTATACC
    TTCACCATCA GCCAAAGTGT GTTGGTCACC AACCTCAAAC CAGCAACCAT CAAAGTCTAT
    GACTACTACC TGCCAGATGA ACAGGCAACA ATTCAGTATT CTGATTCCTG TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803833.1
    CDS40..4416
    Translation

    Target ORF information:

    RefSeq Version XM_010805531.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGCAGTG AATGAAGCTC GTCCAATGGC TTTCAAGCCA
    GCTCCATTCC AGCCAGTTCA GCCGGAAGAG TCTCAGGTTC GCCAGTACTT TCCAGAGACT
    TGGCTGTGGG ATCTGTTTCC TGTTGGCTAC TCGGGAGAGG AGTCTGTCCA CATCACGGTT
    CCTGACACCA TCACCACGTG GAAGGCCATG ACTTTCTGCA CCTCCCAGTC CAAGGGTTTT
    GGCCTCTCAC CCACCGTTGG AGTGACTGCC TTCAAGCCGT TCTTTGTTGA TCTGACGCTC
    CCTTACTCAG TGGTTCGTGG TGAATCCTTC CGTCTGACTG CCACCATCTT CAATTACCTG
    AAGAAGTGCA TCAGGGTTCA GACTAACCTA GCTAAGTCAG AGGAGTACCG AGTGGAAACG
    CAGACAGCCA CTCGGGCCTC CACCTGTCTG TGTGCTGACG AAGCAAAATC CTATCACTGG
    AACATCACGG CTGTCAAACT GGGTCATGTA AACTTCACTA TCAGCACAAA GATTCTGAAC
    AGCAATGAAC TCTGTGGGGG CCAGAAGGCG TTTGTTCCCC TAAAGGGTGG GAGTGACACA
    CTCATCAAAC CAGTTCTGGT CAAGCCGGAA GGAGTCCTTG TGGAGAAGAC ACACAGCTCA
    CTGCTGTGCC CAAAAGGAAA AGTGGCTTCA GAATCTATCT CCCTGGAGGT CCCTGTGGAT
    GTTGTTCCTG ATTCAGCCAG GGCTTATGTT GCTGTTCTGG GAGACATCAT GGGCACAGCC
    CTGCAGAACC TGGACAACTT GGTGCAGATG CCAAGGGGCT GTGGGGAGCA GAACATGGTC
    TTGTTCGCAC CTATCATTTA CGTCCTGCAG TACCTGGAGA AGACAGAGCT GCTGACGGAG
    GAAATCTGGT CTCGAGCAGT GGGCTTCCTG AAGCTGGGGT ACCAGAGGGA ACTGATGTAC
    AAACACAGTA ATGGCTCCTA CAGTGCCTTT GGGGAGCAAG ATGGAAATGG AAACACATGG
    CTGACAGCCT TTGTCACCAA ATGCTTTGGC CAGGCTCGGC AATTCATCTT CATTGACGAC
    AAGAACATCC AGGATGCTCT TGAGTGGATG GCAGGAAACC AGCTCTCCAG TGGCTGCTAC
    GACAACGTGG GAAAGCTCCT TCAAACATCT ATGAAGGGTG GAGTTGATGA TGAGGTCTCC
    TTGACTGCCT ACATTACAGC TGCATTGTTG GAAATGGGCA AGACCATAGA TGACCCAATG
    GTGAGTCAGG GGCTGCAGTG TGTCAAGAGT TCTGTTTCCT CTACCACCAA CCTGTACACA
    CAGGCCCTCC TTGCTTATAC GTTCTCTCTG GCTGGAAACA TGAACATCAG AAACATTCTT
    CTTGAAAAGC TAGATCAACA GGCTATTGTC TCAGGAGAGT CCACTCACTG GAGCCAGAAA
    CCTGCTCCGA CTTCAGATAC CAGCCCTTGG TCTCAGCCTG AGGCTGTGGA TGTGGAACTC
    ACGGCCTATG TATTACTGAC CTTGCTTAGC AAAGATGACC TGACTCAAAA GGAGCTAGGC
    AAGGCCACCA GCATAGTGGC TTGGTTGACC AAGCAGCAAA ATGCATATGG GGGCTTCTCT
    TCCACTCAGG ACACCGTGGT GGCTCTCCAA GCTCTCGCCA AGTATGCCAC CACTGCCTAC
    GTGCCGTCGG AGCAAGTCAA CCTGGCAGTG AAATCCGCTC AGAATTTCCA GCACACCTTC
    AATGTTCAAG CTGCCAACCG TCTGGTGTTC CAGCAGGAGA TGTTGCCCCA TATCCCTGGG
    GTGTACACCT TGGAGGCCTC AGGCAAGGGC TGTATTTATG CGCAGACGGT GCTGAGATAT
    AATATCCTCC CTCCGAAAAG TCTGGAGGCC TTTAGTCTTA GTGTGAAAAC AACGGGATCT
    CAGTGTAAGC AACATACTTC ACTTGGATCC TTGGTGCTGA CGATCCAAAC CAGTTATGTG
    GGGAGTCGCA GCTCTTCCAA TATGGCTATT GTGGAGGTCA AGATGCTTTC TGGATTCAGT
    CCTGTGGAGG GCACCAATCA GTTACTTCTC CAGCAACCAC TGGTGAAGAA GGTTGAATCT
    GAAATTGACA CACTCAACAT CTACTTGGAA GAGCTCGGGA AGGAAACTCA GACCTATACC
    TTCACCATCA GCCAAAGTGT GTTGGTCACC AACCTCAAAC CAGCAACCAT CAAAGTCTAT
    GACTACTACC TGCCAGATGA ACAGGCAACA ATTCAGTATT CTGATTCCTG TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49589
    Clone ID Related Accession (Same CDS sequence) XM_010805531.1 , XM_010805531.2
    Accession Version XM_010805531.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGCAGTG AATGAAGCTC GTCCAATGGC TTTCAAGCCA
    GCTCCATTCC AGCCAGTTCA GCCGGAAGAG TCTCAGGTTC GCCAGTACTT TCCAGAGACT
    TGGCTGTGGG ATCTGTTTCC TGTTGGCTAC TCGGGAGAGG AGTCTGTCCA CATCACGGTT
    CCTGACACCA TCACCACGTG GAAGGCCATG ACTTTCTGCA CCTCCCAGTC CAAGGGTTTT
    GGCCTCTCAC CCACCGTTGG AGTGACTGCC TTCAAGCCGT TCTTTGTTGA TCTGACGCTC
    CCTTACTCAG TGGTTCGTGG TGAATCCTTC CGTCTGACTG CCACCATCTT CAATTACCTG
    AAGAAGTGCA TCAGGGTTCA GACTAACCTA GCTAAGTCAG AGGAGTACCG AGTGGAAACG
    CAGACAGCCA CTCGGGCCTC CACCTGTCTG TGTGCTGACG AAGCAAAATC CTATCACTGG
    AACATCACGG CTGTCAAACT GGGTCATGTA AACTTCACTA TCAGCACAAA GATTCTGAAC
    AGCAATGAAC TCTGTGGGGG CCAGAAGGCG TTTGTTCCCC TAAAGGGTGG GAGTGACACA
    CTCATCAAAC CAGTTCTGGT CAAGCCGGAA GGAGTCCTTG TGGAGAAGAC ACACAGCTCA
    CTGCTGTGCC CAAAAGGAAA AGTGGCTTCA GAATCTATCT CCCTGGAGGT CCCTGTGGAT
    GTTGTTCCTG ATTCAGCCAG GGCTTATGTT GCTGTTCTGG GAGACATCAT GGGCACAGCC
    CTGCAGAACC TGGACAACTT GGTGCAGATG CCAAGGGGCT GTGGGGAGCA GAACATGGTC
    TTGTTCGCAC CTATCATTTA CGTCCTGCAG TACCTGGAGA AGACAGAGCT GCTGACGGAG
    GAAATCTGGT CTCGAGCAGT GGGCTTCCTG AAGCTGGGGT ACCAGAGGGA ACTGATGTAC
    AAACACAGTA ATGGCTCCTA CAGTGCCTTT GGGGAGCAAG ATGGAAATGG AAACACATGG
    CTGACAGCCT TTGTCACCAA ATGCTTTGGC CAGGCTCGGC AATTCATCTT CATTGACGAC
    AAGAACATCC AGGATGCTCT TGAGTGGATG GCAGGAAACC AGCTCTCCAG TGGCTGCTAC
    GACAACGTGG GAAAGCTCCT TCAAACATCT ATGAAGGGTG GAGTTGATGA TGAGGTCTCC
    TTGACTGCCT ACATTACAGC TGCATTGTTG GAAATGGGCA AGACCATAGA TGACCCAATG
    GTGAGTCAGG GGCTGCAGTG TGTCAAGAGT TCTGTTTCCT CTACCACCAA CCTGTACACA
    CAGGCCCTCC TTGCTTATAC GTTCTCTCTG GCTGGAAACA TGAACATCAG AAACATTCTT
    CTTGAAAAGC TAGATCAACA GGCTATTGTC TCAGGAGAGT CCACTCACTG GAGCCAGAAA
    CCTGCTCCGA CTTCAGATAC CAGCCCTTGG TCTCAGCCTG AGGCTGTGGA TGTGGAACTC
    ACGGCCTATG TATTACTGAC CTTGCTTAGC AAAGATGACC TGACTCAAAA GGAGCTAGGC
    AAGGCCACCA GCATAGTGGC TTGGTTGACC AAGCAGCAAA ATGCATATGG GGGCTTCTCT
    TCCACTCAGG ACACCGTGGT GGCTCTCCAA GCTCTCGCCA AGTATGCCAC CACTGCCTAC
    GTGCCGTCGG AGCAAGTCAA CCTGGCAGTG AAATCCGCTC AGAATTTCCA GCACACCTTC
    AATGTTCAAG CTGCCAACCG TCTGGTGTTC CAGCAGGAGA TGTTGCCCCA TATCCCTGGG
    GTGTACACCT TGGAGGCCTC AGGCAAGGGC TGTATTTATG CGCAGACGGT GCTGAGATAT
    AATATCCTCC CTCCGAAAAG TCTGGAGGCC TTTAGTCTTA GTGTGAAAAC AACGGGATCT
    CAGTGTAAGC AACATACTTC ACTTGGATCC TTGGTGCTGA CGATCCAAAC CAGTTATGTG
    GGGAGTCGCA GCTCTTCCAA TATGGCTATT GTGGAGGTCA AGATGCTTTC TGGATTCAGT
    CCTGTGGAGG GCACCAATCA GTTACTTCTC CAGCAACCAC TGGTGAAGAA GGTTGAATCT
    GAAATTGACA CACTCAACAT CTACTTGGAA GAGCTCGGGA AGGAAACTCA GACCTATACC
    TTCACCATCA GCCAAAGTGT GTTGGTCACC AACCTCAAAC CAGCAACCAT CAAAGTCTAT
    GACTACTACC TGCCAGATGA ACAGGCAACA ATTCAGTATT CTGATTCCTG TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803833.1
    CDS128..4504
    Translation

    Target ORF information:

    RefSeq Version XM_010805531.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGCAGTG AATGAAGCTC GTCCAATGGC TTTCAAGCCA
    GCTCCATTCC AGCCAGTTCA GCCGGAAGAG TCTCAGGTTC GCCAGTACTT TCCAGAGACT
    TGGCTGTGGG ATCTGTTTCC TGTTGGCTAC TCGGGAGAGG AGTCTGTCCA CATCACGGTT
    CCTGACACCA TCACCACGTG GAAGGCCATG ACTTTCTGCA CCTCCCAGTC CAAGGGTTTT
    GGCCTCTCAC CCACCGTTGG AGTGACTGCC TTCAAGCCGT TCTTTGTTGA TCTGACGCTC
    CCTTACTCAG TGGTTCGTGG TGAATCCTTC CGTCTGACTG CCACCATCTT CAATTACCTG
    AAGAAGTGCA TCAGGGTTCA GACTAACCTA GCTAAGTCAG AGGAGTACCG AGTGGAAACG
    CAGACAGCCA CTCGGGCCTC CACCTGTCTG TGTGCTGACG AAGCAAAATC CTATCACTGG
    AACATCACGG CTGTCAAACT GGGTCATGTA AACTTCACTA TCAGCACAAA GATTCTGAAC
    AGCAATGAAC TCTGTGGGGG CCAGAAGGCG TTTGTTCCCC TAAAGGGTGG GAGTGACACA
    CTCATCAAAC CAGTTCTGGT CAAGCCGGAA GGAGTCCTTG TGGAGAAGAC ACACAGCTCA
    CTGCTGTGCC CAAAAGGAAA AGTGGCTTCA GAATCTATCT CCCTGGAGGT CCCTGTGGAT
    GTTGTTCCTG ATTCAGCCAG GGCTTATGTT GCTGTTCTGG GAGACATCAT GGGCACAGCC
    CTGCAGAACC TGGACAACTT GGTGCAGATG CCAAGGGGCT GTGGGGAGCA GAACATGGTC
    TTGTTCGCAC CTATCATTTA CGTCCTGCAG TACCTGGAGA AGACAGAGCT GCTGACGGAG
    GAAATCTGGT CTCGAGCAGT GGGCTTCCTG AAGCTGGGGT ACCAGAGGGA ACTGATGTAC
    AAACACAGTA ATGGCTCCTA CAGTGCCTTT GGGGAGCAAG ATGGAAATGG AAACACATGG
    CTGACAGCCT TTGTCACCAA ATGCTTTGGC CAGGCTCGGC AATTCATCTT CATTGACGAC
    AAGAACATCC AGGATGCTCT TGAGTGGATG GCAGGAAACC AGCTCTCCAG TGGCTGCTAC
    GACAACGTGG GAAAGCTCCT TCAAACATCT ATGAAGGGTG GAGTTGATGA TGAGGTCTCC
    TTGACTGCCT ACATTACAGC TGCATTGTTG GAAATGGGCA AGACCATAGA TGACCCAATG
    GTGAGTCAGG GGCTGCAGTG TGTCAAGAGT TCTGTTTCCT CTACCACCAA CCTGTACACA
    CAGGCCCTCC TTGCTTATAC GTTCTCTCTG GCTGGAAACA TGAACATCAG AAACATTCTT
    CTTGAAAAGC TAGATCAACA GGCTATTGTC TCAGGAGAGT CCACTCACTG GAGCCAGAAA
    CCTGCTCCGA CTTCAGATAC CAGCCCTTGG TCTCAGCCTG AGGCTGTGGA TGTGGAACTC
    ACGGCCTATG TATTACTGAC CTTGCTTAGC AAAGATGACC TGACTCAAAA GGAGCTAGGC
    AAGGCCACCA GCATAGTGGC TTGGTTGACC AAGCAGCAAA ATGCATATGG GGGCTTCTCT
    TCCACTCAGG ACACCGTGGT GGCTCTCCAA GCTCTCGCCA AGTATGCCAC CACTGCCTAC
    GTGCCGTCGG AGCAAGTCAA CCTGGCAGTG AAATCCGCTC AGAATTTCCA GCACACCTTC
    AATGTTCAAG CTGCCAACCG TCTGGTGTTC CAGCAGGAGA TGTTGCCCCA TATCCCTGGG
    GTGTACACCT TGGAGGCCTC AGGCAAGGGC TGTATTTATG CGCAGACGGT GCTGAGATAT
    AATATCCTCC CTCCGAAAAG TCTGGAGGCC TTTAGTCTTA GTGTGAAAAC AACGGGATCT
    CAGTGTAAGC AACATACTTC ACTTGGATCC TTGGTGCTGA CGATCCAAAC CAGTTATGTG
    GGGAGTCGCA GCTCTTCCAA TATGGCTATT GTGGAGGTCA AGATGCTTTC TGGATTCAGT
    CCTGTGGAGG GCACCAATCA GTTACTTCTC CAGCAACCAC TGGTGAAGAA GGTTGAATCT
    GAAATTGACA CACTCAACAT CTACTTGGAA GAGCTCGGGA AGGAAACTCA GACCTATACC
    TTCACCATCA GCCAAAGTGT GTTGGTCACC AACCTCAAAC CAGCAACCAT CAAAGTCTAT
    GACTACTACC TGCCAGATGA ACAGGCAACA ATTCAGTATT CTGATTCCTG TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49590
    Clone ID Related Accession (Same CDS sequence) XM_010805532.1 , XM_010805532.2
    Accession Version XM_010805532.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103939.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGCAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803834.1
    CDS40..4413
    Translation

    Target ORF information:

    RefSeq Version XM_010805532.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGCAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49590
    Clone ID Related Accession (Same CDS sequence) XM_010805532.1 , XM_010805532.2
    Accession Version XM_010805532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGCAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803834.1
    CDS24..4397
    Translation

    Target ORF information:

    RefSeq Version XM_010805532.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCAAACT ATCTGGTGGC ATTGCCAGCC CTTCTTAAGT TCCCCTCTAC TCAGAGGGTT
    TGCTTGGATC TGAGTCCTGG GTACGACAAT GTAAAATTCA CTGTTACTCT GGAGACCAAG
    GGCAAGACTC ACACATTGCT GCAACAGTTT GGGCTAAAGA CGAGACACTT ACACTGCTCG
    TCCTTTTTGG TACCACTTCC TTCTGGTGGC ACAGAAGAAG TAGCCACCGT CCGGGTGAGA
    GGAATTGGAC ATAATGTCTA CTTTGAGGAG AAGAAAAAGG TTCTGATTCA GAGGCAGCAG
    AATGGCATCT TTATACAAAC TGACAAACCT GTCTACAGCC CAGGGCAGGA AGTGCATTTC
    CGTATTGTCA CCCTCACCAG CGCCTTTGTT CCAGTGAACG ACCAGTACTC CATTGTGGAA
    CTGCAGGATC CAAATAACAA TAGGATTGCA CAGTGGCTGA ACGTGGTGCC CAAGCAAGGC
    ATCGTAGACC TGTCCTTCCA GCTGGCACCA GAAGCAACGC TGGGCACCTA CAGTGTGGCC
    GTGGCTGATG GCAAGACCTT CGGGACCTTC AGTGTGGAAG AATATGTGCT GCCTAAGTTT
    AAAGTGGAAG TGGTGGAACC CAAAGAGTTA TCAGTGGCGG AAGACTCTTT CTTAGTGAAA
    GTTTGTTGTA GGTACACCTA TGGAAAGCCC ATGCAAGGAG TAGCACACGT ATCAGTGTGT
    CAAAAGCCAT ATACTTACCG GTATCCGGAT CCAGAACGGG AACATTTACC TGACAGATGC
    AAGAAAGTCT CTGGACAGAT TGACAAAGCA GGATGCATTT CAACTTCTGT GGCCATGTCC
    ATGTTCAACC TCACTGGGTA TTTTTACAGA CAAGACATCA ATATCATGGC TACTGTGGTG
    GAGGAAGGGA CAGGAGTGGA GGCCAACACC ACTCGGGATA TATACATTTC TTCACAAATG
    GGATCCATGA CCTTTGAAGA CACCAATGAT TATTATTACC CCAATTTCCC CTTCAGTGGA
    AAGATAAGAG TTAAGGGCCA CGATGGCTCC CTCCTCAAGC ATCATTCAGT GTATCTGGTG
    ATTCATGGAG AAAATGGAGA CATCAACCAG ATCCTAACTA CTGACAACAA TGGCCTCGCT
    GCCTTCAATC TAGACACGGC TAATTGGAAC GGGAAAAGTG TTTCTCTGGA GGGAAGGTTC
    CAGTTGGTAG AAACACATGA TCCAAAGCAA GTGCCTCGTT ACTACCAAAA TGCCTACCTG
    CACCTGCAGC CCTTCTACAA CCCGACTCGC AGCTTCCTTG GCATCCGTCG ACTGAGTGGC
    ATCTTGGAAT GTGGCCGGCC CCAGGAAGTG CTGGTGGATT ACCACATCGA TCCGGCTGAT
    GCAAAGCCTG ACCAGGAAAT CACCTTCTCC TACTATTTAA TAGGGAAAGG AAATTTGGAG
    ATGGAGGGGC AGAAACACCT GAAGTCCAGG ATGAAAGGAC TGAAAGGCTC CTTCTCTCTC
    CCACTGGTCT TCAACTCTAG ATTAGCCCCT GATCCTTCCC TGGTGGTCTA CGCTGTTTTT
    CCCAATGGAG GTGTCATAGC AGACAAAATC CAGTTCTCAG TGGAGATGTG CTTTGACAAT
    CAGGTTTCCC TTGGCTTCTC ACCTTCCCAG CAGCTTCCAG GAGCAGATGT GGACCTCCAG
    CTGCGGGCAG CCCCTGGGTC CCTGTGTGCA GTCCGGGCCG TGGATGAGAG TGTCTTGCTG
    CTTAGACCAG AGAGCGAACT GAGCAATAGC TCGGTCTATG GGATGTTCCC ATTCTGGTAC
    AATCACTACC CCTATCAGGT GGCTGAATAC GATGAGTGTC CAGGGTTTGA TGGTTGGAAC
    GTTCCACAGC CCCTTATTCA GCCAGTGCTC GAGGAGCGTT GGAACAATCG TCTTGTCATG
    TGGAGGCCTT GGATCTCTGA AAAAACAGAC CTTTTCAGTT TGTTCCAGAA CATGGGCCTG
    AAAATACTGT CCAATGCCAA AATCAAGAGG CCAATAGATT GCAGTCACCA GAATTTAAGA
    TACAGCCATA CGATGGCTGA AGCAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49591
    Clone ID Related Accession (Same CDS sequence) XM_010805533.1 , XM_010805533.2
    Accession Version XM_010805533.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103939.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803835.1
    CDS40..4413
    Translation

    Target ORF information:

    RefSeq Version XM_010805533.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin-like 1 (A2ML1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805533.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49591
    Clone ID Related Accession (Same CDS sequence) XM_010805533.1 , XM_010805533.2
    Accession Version XM_010805533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-2-macroglobulin-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803835.1
    CDS54..4427
    Translation

    Target ORF information:

    RefSeq Version XM_010805533.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha-2-macroglobulin like 1 (A2ML1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGGGCTC AGCTCCTTCT AGGAATATTA GCCCTGTTGC CAGCCTCGGC AGGAAAATTT 
    CTCCCTAGAA ACTATCTGGT GGCATTGCCA GCCCTTCTTA AGTTCCCCTC TACTCAGAGG
    GTTTGCTTGG ATCTGAGTCC TGGGTACGAC AATGTAAAAT TCACTGTTAC TCTGGAGACC
    AAGGGCAAGA CTCACACATT GCTGCAACAG TTTGGGCTAA AGACGAGACA CTTACACTGC
    TCGTCCTTTT TGGTACCACT TCCTTCTGGT GGCACAGAAG AAGTAGCCAC CGTCCGGGTG
    AGAGGAATTG GACATAATGT CTACTTTGAG GAGAAGAAAA AGGTTCTGAT TCAGAGGCAG
    CAGAATGGCA TCTTTATACA AACTGACAAA CCTGTCTACA GCCCAGGGCA GGAAGTGCAT
    TTCCGTATTG TCACCCTCAC CAGCGCCTTT GTTCCAGTGA ACGACCAGTA CTCCATTGTG
    GAACTGCAGG ATCCAAATAA CAATAGGATT GCACAGTGGC TGAACGTGGT GCCCAAGCAA
    GGCATCGTAG ACCTGTCCTT CCAGCTGGCA CCAGAAGCAA CGCTGGGCAC CTACAGTGTG
    GCCGTGGCTG ATGGCAAGAC CTTCGGGACC TTCAGTGTGG AAGAATATGT GCTGCCTAAG
    TTTAAAGTGG AAGTGGTGGA ACCCAAAGAG TTATCAGTGG CGGAAGACTC TTTCTTAGTG
    AAAGTTTGTT GTAGGTACAC CTATGGAAAG CCCATGCAAG GAGTAGCACA CGTATCAGTG
    TGTCAAAAGC CATATACTTA CCGGTATCCG GATCCAGAAC GGGAACATTT ACCTGACAGA
    TGCAAGAAAG TCTCTGGACA GATTGACAAA GCAGGATGCA TTTCAACTTC TGTGGCCATG
    TCCATGTTCA ACCTCACTGG GTATTTTTAC AGACAAGACA TCAATATCAT GGCTACTGTG
    GTGGAGGAAG GGACAGGAGT GGAGGCCAAC ACCACTCGGG ATATATACAT TTCTTCACAA
    ATGGGATCCA TGACCTTTGA AGACACCAAT GATTATTATT ACCCCAATTT CCCCTTCAGT
    GGAAAGATAA GAGTTAAGGG CCACGATGGC TCCCTCCTCA AGCATCATTC AGTGTATCTG
    GTGATTCATG GAGAAAATGG AGACATCAAC CAGATCCTAA CTACTGACAA CAATGGCCTC
    GCTGCCTTCA ATCTAGACAC GGCTAATTGG AACGGGAAAA GTGTTTCTCT GGAGGGAAGG
    TTCCAGTTGG TAGAAACACA TGATCCAAAG CAAGTGCCTC GTTACTACCA AAATGCCTAC
    CTGCACCTGC AGCCCTTCTA CAACCCGACT CGCAGCTTCC TTGGCATCCG TCGACTGAGT
    GGCATCTTGG AATGTGGCCG GCCCCAGGAA GTGCTGGTGG ATTACCACAT CGATCCGGCT
    GATGCAAAGC CTGACCAGGA AATCACCTTC TCCTACTATT TAATAGGGAA AGGAAATTTG
    GAGATGGAGG GGCAGAAACA CCTGAAGTCC AGGATGAAAG GACTGAAAGG CTCCTTCTCT
    CTCCCACTGG TCTTCAACTC TAGATTAGCC CCTGATCCTT CCCTGGTGGT CTACGCTGTT
    TTTCCCAATG GAGGTGTCAT AGCAGACAAA ATCCAGTTCT CAGTGGAGAT GTGCTTTGAC
    AATCAGGTTT CCCTTGGCTT CTCACCTTCC CAGCAGCTTC CAGGAGCAGA TGTGGACCTC
    CAGCTGCGGG CAGCCCCTGG GTCCCTGTGT GCAGTCCGGG CCGTGGATGA GAGTGTCTTG
    CTGCTTAGAC CAGAGAGCGA ACTGAGCAAT AGCTCGGTCT ATGGGATGTT CCCATTCTGG
    TACAATCACT ACCCCTATCA GGTGGCTGAA TACGATGAGT GTCCAGGGTT TGATGGTTGG
    AACGTTCCAC AGCCCCTTAT TCAGCCAGTG CTCGAGGAGC GTTGGAACAA TCGTCTTGTC
    ATGTGGAGGC CTTGGATCTC TGAAAAAACA GACCTTTTCA GTTTGTTCCA GAACATGGGC
    CTGAAAATAC TGTCCAATGC CAAAATCAAG AGGCCAATAG ATTGCAGTCA CCAGAATTTA
    AGATACAGCC ATACGATGGC TGAAGTGAAT GAAGCTCGTC CAATGGCTTT CAAGCCAGCT
    CCATTCCAGC CAGTTCAGCC GGAAGAGTCT CAGGTTCGCC AGTACTTTCC AGAGACTTGG
    CTGTGGGATC TGTTTCCTGT TGGCTACTCG GGAGAGGAGT CTGTCCACAT CACGGTTCCT
    GACACCATCA CCACGTGGAA GGCCATGACT TTCTGCACCT CCCAGTCCAA GGGTTTTGGC
    CTCTCACCCA CCGTTGGAGT GACTGCCTTC AAGCCGTTCT TTGTTGATCT GACGCTCCCT
    TACTCAGTGG TTCGTGGTGA ATCCTTCCGT CTGACTGCCA CCATCTTCAA TTACCTGAAG
    AAGTGCATCA GGGTTCAGAC TAACCTAGCT AAGTCAGAGG AGTACCGAGT GGAAACGCAG
    ACAGCCACTC GGGCCTCCAC CTGTCTGTGT GCTGACGAAG CAAAATCCTA TCACTGGAAC
    ATCACGGCTG TCAAACTGGG TCATGTAAAC TTCACTATCA GCACAAAGAT TCTGAACAGC
    AATGAACTCT GTGGGGGCCA GAAGGCGTTT GTTCCCCTAA AGGGTGGGAG TGACACACTC
    ATCAAACCAG TTCTGGTCAA GCCGGAAGGA GTCCTTGTGG AGAAGACACA CAGCTCACTG
    CTGTGCCCAA AAGGAAAAGT GGCTTCAGAA TCTATCTCCC TGGAGGTCCC TGTGGATGTT
    GTTCCTGATT CAGCCAGGGC TTATGTTGCT GTTCTGGGAG ACATCATGGG CACAGCCCTG
    CAGAACCTGG ACAACTTGGT GCAGATGCCA AGGGGCTGTG GGGAGCAGAA CATGGTCTTG
    TTCGCACCTA TCATTTACGT CCTGCAGTAC CTGGAGAAGA CAGAGCTGCT GACGGAGGAA
    ATCTGGTCTC GAGCAGTGGG CTTCCTGAAG CTGGGGTACC AGAGGGAACT GATGTACAAA
    CACAGTAATG GCTCCTACAG TGCCTTTGGG GAGCAAGATG GAAATGGAAA CACATGGCTG
    ACAGCCTTTG TCACCAAATG CTTTGGCCAG GCTCGGCAAT TCATCTTCAT TGACGACAAG
    AACATCCAGG ATGCTCTTGA GTGGATGGCA GGAAACCAGC TCTCCAGTGG CTGCTACGAC
    AACGTGGGAA AGCTCCTTCA AACATCTATG AAGGGTGGAG TTGATGATGA GGTCTCCTTG
    ACTGCCTACA TTACAGCTGC ATTGTTGGAA ATGGGCAAGA CCATAGATGA CCCAATGGTG
    AGTCAGGGGC TGCAGTGTGT CAAGAGTTCT GTTTCCTCTA CCACCAACCT GTACACACAG
    GCCCTCCTTG CTTATACGTT CTCTCTGGCT GGAAACATGA ACATCAGAAA CATTCTTCTT
    GAAAAGCTAG ATCAACAGGC TATTGTCTCA GGAGAGTCCA CTCACTGGAG CCAGAAACCT
    GCTCCGACTT CAGATACCAG CCCTTGGTCT CAGCCTGAGG CTGTGGATGT GGAACTCACG
    GCCTATGTAT TACTGACCTT GCTTAGCAAA GATGACCTGA CTCAAAAGGA GCTAGGCAAG
    GCCACCAGCA TAGTGGCTTG GTTGACCAAG CAGCAAAATG CATATGGGGG CTTCTCTTCC
    ACTCAGGACA CCGTGGTGGC TCTCCAAGCT CTCGCCAAGT ATGCCACCAC TGCCTACGTG
    CCGTCGGAGC AAGTCAACCT GGCAGTGAAA TCCGCTCAGA ATTTCCAGCA CACCTTCAAT
    GTTCAAGCTG CCAACCGTCT GGTGTTCCAG CAGGAGATGT TGCCCCATAT CCCTGGGGTG
    TACACCTTGG AGGCCTCAGG CAAGGGCTGT ATTTATGCGC AGACGGTGCT GAGATATAAT
    ATCCTCCCTC CGAAAAGTCT GGAGGCCTTT AGTCTTAGTG TGAAAACAAC GGGATCTCAG
    TGTAAGCAAC ATACTTCACT TGGATCCTTG GTGCTGACGA TCCAAACCAG TTATGTGGGG
    AGTCGCAGCT CTTCCAATAT GGCTATTGTG GAGGTCAAGA TGCTTTCTGG ATTCAGTCCT
    GTGGAGGGCA CCAATCAGTT ACTTCTCCAG CAACCACTGG TGAAGAAGGT TGAATCTGAA
    ATTGACACAC TCAACATCTA CTTGGAAGAG CTCGGGAAGG AAACTCAGAC CTATACCTTC
    ACCATCAGCC AAAGTGTGTT GGTCACCAAC CTCAAACCAG CAACCATCAA AGTCTATGAC
    TACTACCTGC CAGATGAACA GGCAACAATT CAGTATTCTG ATTCCTGTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.