vps8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following vps8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa16037 XM_002936535.4
Latest version!
Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa30970 XM_012970873.2
Latest version!
Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa30971 XM_012970874.3
Latest version!
Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
OXa30970 XM_012970873.1 Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa16037 XM_002936535.3 Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa30971 XM_012970874.2 Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa16037
    Clone ID Related Accession (Same CDS sequence) XM_002936535.3 , XM_002936535.4
    Accession Version XM_002936535.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936535.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936581.2
    CDS28..4374
    Translation

    Target ORF information:

    RefSeq Version XM_002936535.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936535.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30970
    Clone ID Related Accession (Same CDS sequence) XM_012970873.2 , XM_012970873.1
    Accession Version XM_012970873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGGCAAA
    GACACCAATC AGGCCATGAG GCTCTGCCTG GGGAGCACAG CAGTAGGGGC ACAATATGGA
    GCTGTCTCTG CTCTCAGTAT CAACAGTGAC TGCTCCAGAT TACTCTGTGG CTTTGCCAAA
    GGACAGATTA CCATGTGGGA CCTGGCTGGT GGTAAACTTT TAAGAACCAT AACAGATGCC
    CACCCTCCTG GAACTGCAAT TTTACATATC AAGTTCACAG ATGACCCAAC TCTTGCAGTC
    TGCAATGATA GTGGAGGATC CGTATTCGAG TTAACTTTTA AGAGAGTAAT GGGAGTGCGA
    ACCTGTGAGT CCCGTTGCCT TTTCAGTGGA TCCAAGGGAG AAGTTTGCTG TATTGAACCT
    CTGCATACCA AAGCTGAGCT ACGCGATCAC CCAATAACCC ACTACTCATT GCTGGCCATG
    GCATCTCTAA CCAAGATTCT TGTCATTGGC TTGAAACCCT CTCTGAAAGT CTGGATGACC
    TTTCCCTATG GACGGATGGA TCCTTCCAGC GTCCCACTGC TGGCCTGGCA CTTTGTTATC
    TTGCAGGGAG CCCTAAACCC CATGCTTGCA TTTTGCAGGG GGGATGTTGT ACATTTCCTC
    TTAGTAAAAA GGGATGATTC TGGAGCTATT CATATCAATA AACAGAAACA ACTGCATTTG
    CACTATGACC TTATCAACTT CACTTGGATC AATGCTCGCA CGGTGGCTCT TCTCGACAGC
    GCAGAAAAGC TACACGTAAT CGATCGTCAG ACACAGGAAG AACTTGAGAC CCTGGAGATA
    TCAGATGTGC AACTTGTATA CAACAGCAGC CACTTTAAGT CTCTGGCCAC TGGAGGAAAT
    GTGAGCCAGG CCCTGGCCTT TGTGGGAGAG AAAGCATGCT ATGAATCAAT TAGCAGCTAT
    GGAGGACAGA TCTTCTACCT GGGAACAAAG TCAGTCCATG CCATGACGCT GAGAAACTGG
    CGAGAGAGAG TTGACCATTT ACTGAAGCAG GACTGTTATA ATGAAGCTTT GGCATTAGCA
    TGGTCTTTTC ATGAGGGCAA AGCTAAAGCT GTTGTGGGGC TGTCTGGAGA CCCAAAGAAG
    AGGAAGAGTG TTGTAGCTGA TAAGATTGTT GAAATCTTAC TCCAGTATAC TGAAAAAGCA
    CTAAGAAAAT GTCCAGAGCA AGGAAAAATC CAGGTGATGG AACAGCATTT CCAGGACACT
    GTGCCATTAA TGGTGGATTA TTGCCTTCTA TTACAGCGCA CGGATATCCT TTTTAGCCAG
    ATGTATGACA AGTTAAGTGA GAACTGTGTG GCCAAAGGTG TTTTCTTGGA GTGCTTAGAA
    CCATACCTGT TACGGGACCA GCTGCCAGGG CTGACTGCAC ATGTCATGAA GGATCTGCTC
    CTCCATTTCC AGGACCAAGG GCAGATGGAA AGTGTTGAGG CTTGTATTGT GCACATGGAC
    ATCACCAGCC TGGACATACA AAATATTGTC CACATGTGCT GGGAACATAG ACTTTATGAT
    GCAGTGATCT ATGTGTACAA CAAAGGAACA AATGATTTTA TTAGTCCAAT GGAGAAACTA
    TTTAATGCCA TCTCTGCACC TATCAACTCT GGGAAGTCAC TAACTGATGA GCAAGTTGTG
    ATGGGAAACA AGCTGCTGGT TTACATCAGT TCCAGTTTGG CTGGAAGAGC ATATCCTTTG
    GGGGATATTC CAGAAGACCT TGTGCCTTTG GTGAAAAATC AGGTTTTTGA GTTCCTGATC
    CGTTTGCACT CTCCAGAGAC CCCACAAGAA GAGGAGGTGT ATCCATATAT ACGTACCCTG
    CTGCATTTTG ATACACGGGA GTTCCTCAAT GTACTAGCCT TGACATTTGA AGACTTTACA
    AATGATAAGC AAGCAGTGGA ACATCAGCAA CGTATTGTGG ATATTCTTCT GAAGGTCATG
    GTGGAAAACT CTGACTTTAC TCCATCCCAA GTCGGCTGCC TCTTTACTTT CCTAGCCCGA
    CAGCTTGCCA AGCCAGATAA TACACTTTTT GTGAACCGAA TGTTGTTTGA CCAGGTTTTA
    GAATTCCTGT GTAGTCCAGA TGATGATTCC CGACATTCTG AACGACAGCA GGTTCTCCTT
    GAACTTCTTC ATGCTGGAGG AGTTGTGCAA TTTGAAGAGA ACAGACTGAT CCAGCTAGCA
    GAAAGAGCAC AATTCTATCA GATCTGTGAG TTTATGTATG AGCGGAAACA CCAGTATGAT
    AAAATCATAG ACTGCTACAT GCAAGACCCC ATACGGAAGC AAGATGTATT CAACTACATT
    CATAACATCT TATCAATCCC TGGTTATAGT GCTGAGGAGA AGGAAGCCAC ATGGGACAAA
    ACCATGCAGC ACATGGAGGA ACTAATATTG TTATGCCCTA GTAAAGCAGC TGACCTTGTA
    TGTACACACT TTAGCAGCCA TTTGGAAGAT GTCATTGGAA AGCTCAAGGA TCCGTTATTG
    GTTTTACAGT TCCTTAGAAG TCTCCTTGAT CCAAGGGATG GAGCCCATGT GACTCCTGAA
    GTATTACAAA CTCTTCCACA CATCACAGAG CTTTTCATAG ATTTGATGTA TCAAAATGAT
    CCAGAACAGG TGGCTCCACT GCTGCAGATC CTGGAACACT ATAGACTTGA GGAGACGATA
    CAGATTGCAC AAAGACACAA TCATCATGAA ACCTTGGCAT ATCTCCTGGA GAAGAAAGGG
    GATGTTGAAG CTGCTTTCCG AGTCATGCTT ATGAGACTGC AAGAAAGGCT CTCCAATCTG
    ACCTCAGATT CTGTGGACTC ACCAAGCTTC AAAGTAATGC CCAACATGAA AGATTTAGAA
    GAAACCTTGA CAGAGACCAT TTTACTGTGT GAAAGAACTT CAGACACCCT AAACCAGCAA
    CAGCGAGAGG CATTATGGTT TCCTTTGCTA GAAGCAATGA TGTCTCCGCA AAAGCACTTC
    ACTTCATCTT CTGCACAAAT GTACTCCGAA TCTTTAAAAA CTCTGACTAT GCAAGTTCTG
    AACAGCATGG CCACGTACAT CTCTCTTCCG TCAATCCTCC AAAGAATCCT TCAGGACCCT
    GTATATGGGA AAGGTAAACT AGTTGAAATC CAAGGTCTGG TTTTGGGAAT GCTAGACACA
    TTCAACTATG AACAGACCCT GCTGGAAACT ACTACTAATC TGCTGAACCA AGACCTGCAC
    TGGTCTCTTT GCAACTTGAA GGCTTCAGTC ACCCGGGCTC TTGTTCCCAA ACAGGACTAC
    TGCTGTGTGT GTCTGCAGCA ATACAAGAGG AGACAGGAAG CCACTGATGA AATCATTGTA
    TTCAGCTGTG GCCACCTTTA CCATTCCCTT TGCCTCCTGG GCAAGGACTG CAAGGTTGAA
    GTTGGAGGAC AGAGTCAGTG GATCTGCCAT CGGTGTAACT CAACTAACAA AGGCCGTGCG
    GGGAGGCAGA CAGTGACAGA TGTGAAGAAA GGCCGAACTA CCTCATTAGC GCAGGTGAGA
    GGGACTTCAT CTCGATCATC AAGAGAGGAG GCTGCCAAGA AGTCGAGCAC TGATCCCATT
    CTAGATCCAC AGCAGCTGCA GGCGTTCCAG CATCTCTGCC GTCTCTACAA AGGAACCTCA
    CGGTTGGCTC TCCTTACTGA GCTATGCCGC TCTCAAAGCC CAGAAAAATC ATCTTCATAT
    TATGCTTCCT ATGGTGGAAT TGCTACAAGT AGTGTTTTCC AGAATGAGAA CTTCCAACTG
    CACCTTGCCC CACCCCCTTA CCTTGGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826327.1
    CDS243..4595
    Translation

    Target ORF information:

    RefSeq Version XM_012970873.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGGCAAA
    GACACCAATC AGGCCATGAG GCTCTGCCTG GGGAGCACAG CAGTAGGGGC ACAATATGGA
    GCTGTCTCTG CTCTCAGTAT CAACAGTGAC TGCTCCAGAT TACTCTGTGG CTTTGCCAAA
    GGACAGATTA CCATGTGGGA CCTGGCTGGT GGTAAACTTT TAAGAACCAT AACAGATGCC
    CACCCTCCTG GAACTGCAAT TTTACATATC AAGTTCACAG ATGACCCAAC TCTTGCAGTC
    TGCAATGATA GTGGAGGATC CGTATTCGAG TTAACTTTTA AGAGAGTAAT GGGAGTGCGA
    ACCTGTGAGT CCCGTTGCCT TTTCAGTGGA TCCAAGGGAG AAGTTTGCTG TATTGAACCT
    CTGCATACCA AAGCTGAGCT ACGCGATCAC CCAATAACCC ACTACTCATT GCTGGCCATG
    GCATCTCTAA CCAAGATTCT TGTCATTGGC TTGAAACCCT CTCTGAAAGT CTGGATGACC
    TTTCCCTATG GACGGATGGA TCCTTCCAGC GTCCCACTGC TGGCCTGGCA CTTTGTTATC
    TTGCAGGGAG CCCTAAACCC CATGCTTGCA TTTTGCAGGG GGGATGTTGT ACATTTCCTC
    TTAGTAAAAA GGGATGATTC TGGAGCTATT CATATCAATA AACAGAAACA ACTGCATTTG
    CACTATGACC TTATCAACTT CACTTGGATC AATGCTCGCA CGGTGGCTCT TCTCGACAGC
    GCAGAAAAGC TACACGTAAT CGATCGTCAG ACACAGGAAG AACTTGAGAC CCTGGAGATA
    TCAGATGTGC AACTTGTATA CAACAGCAGC CACTTTAAGT CTCTGGCCAC TGGAGGAAAT
    GTGAGCCAGG CCCTGGCCTT TGTGGGAGAG AAAGCATGCT ATGAATCAAT TAGCAGCTAT
    GGAGGACAGA TCTTCTACCT GGGAACAAAG TCAGTCCATG CCATGACGCT GAGAAACTGG
    CGAGAGAGAG TTGACCATTT ACTGAAGCAG GACTGTTATA ATGAAGCTTT GGCATTAGCA
    TGGTCTTTTC ATGAGGGCAA AGCTAAAGCT GTTGTGGGGC TGTCTGGAGA CCCAAAGAAG
    AGGAAGAGTG TTGTAGCTGA TAAGATTGTT GAAATCTTAC TCCAGTATAC TGAAAAAGCA
    CTAAGAAAAT GTCCAGAGCA AGGAAAAATC CAGGTGATGG AACAGCATTT CCAGGACACT
    GTGCCATTAA TGGTGGATTA TTGCCTTCTA TTACAGCGCA CGGATATCCT TTTTAGCCAG
    ATGTATGACA AGTTAAGTGA GAACTGTGTG GCCAAAGGTG TTTTCTTGGA GTGCTTAGAA
    CCATACCTGT TACGGGACCA GCTGCCAGGG CTGACTGCAC ATGTCATGAA GGATCTGCTC
    CTCCATTTCC AGGACCAAGG GCAGATGGAA AGTGTTGAGG CTTGTATTGT GCACATGGAC
    ATCACCAGCC TGGACATACA AAATATTGTC CACATGTGCT GGGAACATAG ACTTTATGAT
    GCAGTGATCT ATGTGTACAA CAAAGGAACA AATGATTTTA TTAGTCCAAT GGAGAAACTA
    TTTAATGCCA TCTCTGCACC TATCAACTCT GGGAAGTCAC TAACTGATGA GCAAGTTGTG
    ATGGGAAACA AGCTGCTGGT TTACATCAGT TCCAGTTTGG CTGGAAGAGC ATATCCTTTG
    GGGGATATTC CAGAAGACCT TGTGCCTTTG GTGAAAAATC AGGTTTTTGA GTTCCTGATC
    CGTTTGCACT CTCCAGAGAC CCCACAAGAA GAGGAGGTGT ATCCATATAT ACGTACCCTG
    CTGCATTTTG ATACACGGGA GTTCCTCAAT GTACTAGCCT TGACATTTGA AGACTTTACA
    AATGATAAGC AAGCAGTGGA ACATCAGCAA CGTATTGTGG ATATTCTTCT GAAGGTCATG
    GTGGAAAACT CTGACTTTAC TCCATCCCAA GTCGGCTGCC TCTTTACTTT CCTAGCCCGA
    CAGCTTGCCA AGCCAGATAA TACACTTTTT GTGAACCGAA TGTTGTTTGA CCAGGTTTTA
    GAATTCCTGT GTAGTCCAGA TGATGATTCC CGACATTCTG AACGACAGCA GGTTCTCCTT
    GAACTTCTTC ATGCTGGAGG AGTTGTGCAA TTTGAAGAGA ACAGACTGAT CCAGCTAGCA
    GAAAGAGCAC AATTCTATCA GATCTGTGAG TTTATGTATG AGCGGAAACA CCAGTATGAT
    AAAATCATAG ACTGCTACAT GCAAGACCCC ATACGGAAGC AAGATGTATT CAACTACATT
    CATAACATCT TATCAATCCC TGGTTATAGT GCTGAGGAGA AGGAAGCCAC ATGGGACAAA
    ACCATGCAGC ACATGGAGGA ACTAATATTG TTATGCCCTA GTAAAGCAGC TGACCTTGTA
    TGTACACACT TTAGCAGCCA TTTGGAAGAT GTCATTGGAA AGCTCAAGGA TCCGTTATTG
    GTTTTACAGT TCCTTAGAAG TCTCCTTGAT CCAAGGGATG GAGCCCATGT GACTCCTGAA
    GTATTACAAA CTCTTCCACA CATCACAGAG CTTTTCATAG ATTTGATGTA TCAAAATGAT
    CCAGAACAGG TGGCTCCACT GCTGCAGATC CTGGAACACT ATAGACTTGA GGAGACGATA
    CAGATTGCAC AAAGACACAA TCATCATGAA ACCTTGGCAT ATCTCCTGGA GAAGAAAGGG
    GATGTTGAAG CTGCTTTCCG AGTCATGCTT ATGAGACTGC AAGAAAGGCT CTCCAATCTG
    ACCTCAGATT CTGTGGACTC ACCAAGCTTC AAAGTAATGC CCAACATGAA AGATTTAGAA
    GAAACCTTGA CAGAGACCAT TTTACTGTGT GAAAGAACTT CAGACACCCT AAACCAGCAA
    CAGCGAGAGG CATTATGGTT TCCTTTGCTA GAAGCAATGA TGTCTCCGCA AAAGCACTTC
    ACTTCATCTT CTGCACAAAT GTACTCCGAA TCTTTAAAAA CTCTGACTAT GCAAGTTCTG
    AACAGCATGG CCACGTACAT CTCTCTTCCG TCAATCCTCC AAAGAATCCT TCAGGACCCT
    GTATATGGGA AAGGTAAACT AGTTGAAATC CAAGGTCTGG TTTTGGGAAT GCTAGACACA
    TTCAACTATG AACAGACCCT GCTGGAAACT ACTACTAATC TGCTGAACCA AGACCTGCAC
    TGGTCTCTTT GCAACTTGAA GGCTTCAGTC ACCCGGGCTC TTGTTCCCAA ACAGGACTAC
    TGCTGTGTGT GTCTGCAGCA ATACAAGAGG AGACAGGAAG CCACTGATGA AATCATTGTA
    TTCAGCTGTG GCCACCTTTA CCATTCCCTT TGCCTCCTGG GCAAGGACTG CAAGGTTGAA
    GTTGGAGGAC AGAGTCAGTG GATCTGCCAT CGGTGTAACT CAACTAACAA AGGCCGTGCG
    GGGAGGCAGA CAGTGACAGA TGTGAAGAAA GGCCGAACTA CCTCATTAGC GCAGGTGAGA
    GGGACTTCAT CTCGATCATC AAGAGAGGAG GCTGCCAAGA AGTCGAGCAC TGATCCCATT
    CTAGATCCAC AGCAGCTGCA GGCGTTCCAG CATCTCTGCC GTCTCTACAA AGGAACCTCA
    CGGTTGGCTC TCCTTACTGA GCTATGCCGC TCTCAAAGCC CAGAAAAATC ATCTTCATAT
    TATGCTTCCT ATGGTGGAAT TGCTACAAGT AGTGTTTTCC AGAATGAGAA CTTCCAACTG
    CACCTTGCCC CACCCCCTTA CCTTGGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30971
    Clone ID Related Accession (Same CDS sequence) XM_012970874.3 , XM_012970874.2
    Accession Version XM_012970874.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970874.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT 
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826328.1
    CDS252..4538
    Translation

    Target ORF information:

    RefSeq Version XM_012970874.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970874.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT 
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa16037
    Clone ID Related Accession (Same CDS sequence) XM_002936535.3 , XM_002936535.4
    Accession Version XM_002936535.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002936535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936581.2
    CDS25..4371
    Translation

    Target ORF information:

    RefSeq Version XM_002936535.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936535.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30970
    Clone ID Related Accession (Same CDS sequence) XM_012970873.2 , XM_012970873.1
    Accession Version XM_012970873.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGGCAAA
    GACACCAATC AGGCCATGAG GCTCTGCCTG GGGAGCACAG CAGTAGGGGC ACAATATGGA
    GCTGTCTCTG CTCTCAGTAT CAACAGTGAC TGCTCCAGAT TACTCTGTGG CTTTGCCAAA
    GGACAGATTA CCATGTGGGA CCTGGCTGGT GGTAAACTTT TAAGAACCAT AACAGATGCC
    CACCCTCCTG GAACTGCAAT TTTACATATC AAGTTCACAG ATGACCCAAC TCTTGCAGTC
    TGCAATGATA GTGGAGGATC CGTATTCGAG TTAACTTTTA AGAGAGTAAT GGGAGTGCGA
    ACCTGTGAGT CCCGTTGCCT TTTCAGTGGA TCCAAGGGAG AAGTTTGCTG TATTGAACCT
    CTGCATACCA AAGCTGAGCT ACGCGATCAC CCAATAACCC ACTACTCATT GCTGGCCATG
    GCATCTCTAA CCAAGATTCT TGTCATTGGC TTGAAACCCT CTCTGAAAGT CTGGATGACC
    TTTCCCTATG GACGGATGGA TCCTTCCAGC GTCCCACTGC TGGCCTGGCA CTTTGTTATC
    TTGCAGGGAG CCCTAAACCC CATGCTTGCA TTTTGCAGGG GGGATGTTGT ACATTTCCTC
    TTAGTAAAAA GGGATGATTC TGGAGCTATT CATATCAATA AACAGAAACA ACTGCATTTG
    CACTATGACC TTATCAACTT CACTTGGATC AATGCTCGCA CGGTGGCTCT TCTCGACAGC
    GCAGAAAAGC TACACGTAAT CGATCGTCAG ACACAGGAAG AACTTGAGAC CCTGGAGATA
    TCAGATGTGC AACTTGTATA CAACAGCAGC CACTTTAAGT CTCTGGCCAC TGGAGGAAAT
    GTGAGCCAGG CCCTGGCCTT TGTGGGAGAG AAAGCATGCT ATGAATCAAT TAGCAGCTAT
    GGAGGACAGA TCTTCTACCT GGGAACAAAG TCAGTCCATG CCATGACGCT GAGAAACTGG
    CGAGAGAGAG TTGACCATTT ACTGAAGCAG GACTGTTATA ATGAAGCTTT GGCATTAGCA
    TGGTCTTTTC ATGAGGGCAA AGCTAAAGCT GTTGTGGGGC TGTCTGGAGA CCCAAAGAAG
    AGGAAGAGTG TTGTAGCTGA TAAGATTGTT GAAATCTTAC TCCAGTATAC TGAAAAAGCA
    CTAAGAAAAT GTCCAGAGCA AGGAAAAATC CAGGTGATGG AACAGCATTT CCAGGACACT
    GTGCCATTAA TGGTGGATTA TTGCCTTCTA TTACAGCGCA CGGATATCCT TTTTAGCCAG
    ATGTATGACA AGTTAAGTGA GAACTGTGTG GCCAAAGGTG TTTTCTTGGA GTGCTTAGAA
    CCATACCTGT TACGGGACCA GCTGCCAGGG CTGACTGCAC ATGTCATGAA GGATCTGCTC
    CTCCATTTCC AGGACCAAGG GCAGATGGAA AGTGTTGAGG CTTGTATTGT GCACATGGAC
    ATCACCAGCC TGGACATACA AAATATTGTC CACATGTGCT GGGAACATAG ACTTTATGAT
    GCAGTGATCT ATGTGTACAA CAAAGGAACA AATGATTTTA TTAGTCCAAT GGAGAAACTA
    TTTAATGCCA TCTCTGCACC TATCAACTCT GGGAAGTCAC TAACTGATGA GCAAGTTGTG
    ATGGGAAACA AGCTGCTGGT TTACATCAGT TCCAGTTTGG CTGGAAGAGC ATATCCTTTG
    GGGGATATTC CAGAAGACCT TGTGCCTTTG GTGAAAAATC AGGTTTTTGA GTTCCTGATC
    CGTTTGCACT CTCCAGAGAC CCCACAAGAA GAGGAGGTGT ATCCATATAT ACGTACCCTG
    CTGCATTTTG ATACACGGGA GTTCCTCAAT GTACTAGCCT TGACATTTGA AGACTTTACA
    AATGATAAGC AAGCAGTGGA ACATCAGCAA CGTATTGTGG ATATTCTTCT GAAGGTCATG
    GTGGAAAACT CTGACTTTAC TCCATCCCAA GTCGGCTGCC TCTTTACTTT CCTAGCCCGA
    CAGCTTGCCA AGCCAGATAA TACACTTTTT GTGAACCGAA TGTTGTTTGA CCAGGTTTTA
    GAATTCCTGT GTAGTCCAGA TGATGATTCC CGACATTCTG AACGACAGCA GGTTCTCCTT
    GAACTTCTTC ATGCTGGAGG AGTTGTGCAA TTTGAAGAGA ACAGACTGAT CCAGCTAGCA
    GAAAGAGCAC AATTCTATCA GATCTGTGAG TTTATGTATG AGCGGAAACA CCAGTATGAT
    AAAATCATAG ACTGCTACAT GCAAGACCCC ATACGGAAGC AAGATGTATT CAACTACATT
    CATAACATCT TATCAATCCC TGGTTATAGT GCTGAGGAGA AGGAAGCCAC ATGGGACAAA
    ACCATGCAGC ACATGGAGGA ACTAATATTG TTATGCCCTA GTAAAGCAGC TGACCTTGTA
    TGTACACACT TTAGCAGCCA TTTGGAAGAT GTCATTGGAA AGCTCAAGGA TCCGTTATTG
    GTTTTACAGT TCCTTAGAAG TCTCCTTGAT CCAAGGGATG GAGCCCATGT GACTCCTGAA
    GTATTACAAA CTCTTCCACA CATCACAGAG CTTTTCATAG ATTTGATGTA TCAAAATGAT
    CCAGAACAGG TGGCTCCACT GCTGCAGATC CTGGAACACT ATAGACTTGA GGAGACGATA
    CAGATTGCAC AAAGACACAA TCATCATGAA ACCTTGGCAT ATCTCCTGGA GAAGAAAGGG
    GATGTTGAAG CTGCTTTCCG AGTCATGCTT ATGAGACTGC AAGAAAGGCT CTCCAATCTG
    ACCTCAGATT CTGTGGACTC ACCAAGCTTC AAAGTAATGC CCAACATGAA AGATTTAGAA
    GAAACCTTGA CAGAGACCAT TTTACTGTGT GAAAGAACTT CAGACACCCT AAACCAGCAA
    CAGCGAGAGG CATTATGGTT TCCTTTGCTA GAAGCAATGA TGTCTCCGCA AAAGCACTTC
    ACTTCATCTT CTGCACAAAT GTACTCCGAA TCTTTAAAAA CTCTGACTAT GCAAGTTCTG
    AACAGCATGG CCACGTACAT CTCTCTTCCG TCAATCCTCC AAAGAATCCT TCAGGACCCT
    GTATATGGGA AAGGTAAACT AGTTGAAATC CAAGGTCTGG TTTTGGGAAT GCTAGACACA
    TTCAACTATG AACAGACCCT GCTGGAAACT ACTACTAATC TGCTGAACCA AGACCTGCAC
    TGGTCTCTTT GCAACTTGAA GGCTTCAGTC ACCCGGGCTC TTGTTCCCAA ACAGGACTAC
    TGCTGTGTGT GTCTGCAGCA ATACAAGAGG AGACAGGAAG CCACTGATGA AATCATTGTA
    TTCAGCTGTG GCCACCTTTA CCATTCCCTT TGCCTCCTGG GCAAGGACTG CAAGGTTGAA
    GTTGGAGGAC AGAGTCAGTG GATCTGCCAT CGGTGTAACT CAACTAACAA AGGCCGTGCG
    GGGAGGCAGA CAGTGACAGA TGTGAAGAAA GGCCGAACTA CCTCATTAGC GCAGGTGAGA
    GGGACTTCAT CTCGATCATC AAGAGAGGAG GCTGCCAAGA AGTCGAGCAC TGATCCCATT
    CTAGATCCAC AGCAGCTGCA GGCGTTCCAG CATCTCTGCC GTCTCTACAA AGGAACCTCA
    CGGTTGGCTC TCCTTACTGA GCTATGCCGC TCTCAAAGCC CAGAAAAATC ATCTTCATAT
    TATGCTTCCT ATGGTGGAAT TGCTACAAGT AGTGTTTTCC AGAATGAGAA CTTCCAACTG
    CACCTTGCCC CACCCCCTTA CCTTGGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826327.1
    CDS25..4377
    Translation

    Target ORF information:

    RefSeq Version XM_012970873.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTTAGTT CATGTTCCGA GACCTTGGCG CACAGAGAGA GAGTCATCAC CCCAACAGGA 
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGGCAAA
    GACACCAATC AGGCCATGAG GCTCTGCCTG GGGAGCACAG CAGTAGGGGC ACAATATGGA
    GCTGTCTCTG CTCTCAGTAT CAACAGTGAC TGCTCCAGAT TACTCTGTGG CTTTGCCAAA
    GGACAGATTA CCATGTGGGA CCTGGCTGGT GGTAAACTTT TAAGAACCAT AACAGATGCC
    CACCCTCCTG GAACTGCAAT TTTACATATC AAGTTCACAG ATGACCCAAC TCTTGCAGTC
    TGCAATGATA GTGGAGGATC CGTATTCGAG TTAACTTTTA AGAGAGTAAT GGGAGTGCGA
    ACCTGTGAGT CCCGTTGCCT TTTCAGTGGA TCCAAGGGAG AAGTTTGCTG TATTGAACCT
    CTGCATACCA AAGCTGAGCT ACGCGATCAC CCAATAACCC ACTACTCATT GCTGGCCATG
    GCATCTCTAA CCAAGATTCT TGTCATTGGC TTGAAACCCT CTCTGAAAGT CTGGATGACC
    TTTCCCTATG GACGGATGGA TCCTTCCAGC GTCCCACTGC TGGCCTGGCA CTTTGTTATC
    TTGCAGGGAG CCCTAAACCC CATGCTTGCA TTTTGCAGGG GGGATGTTGT ACATTTCCTC
    TTAGTAAAAA GGGATGATTC TGGAGCTATT CATATCAATA AACAGAAACA ACTGCATTTG
    CACTATGACC TTATCAACTT CACTTGGATC AATGCTCGCA CGGTGGCTCT TCTCGACAGC
    GCAGAAAAGC TACACGTAAT CGATCGTCAG ACACAGGAAG AACTTGAGAC CCTGGAGATA
    TCAGATGTGC AACTTGTATA CAACAGCAGC CACTTTAAGT CTCTGGCCAC TGGAGGAAAT
    GTGAGCCAGG CCCTGGCCTT TGTGGGAGAG AAAGCATGCT ATGAATCAAT TAGCAGCTAT
    GGAGGACAGA TCTTCTACCT GGGAACAAAG TCAGTCCATG CCATGACGCT GAGAAACTGG
    CGAGAGAGAG TTGACCATTT ACTGAAGCAG GACTGTTATA ATGAAGCTTT GGCATTAGCA
    TGGTCTTTTC ATGAGGGCAA AGCTAAAGCT GTTGTGGGGC TGTCTGGAGA CCCAAAGAAG
    AGGAAGAGTG TTGTAGCTGA TAAGATTGTT GAAATCTTAC TCCAGTATAC TGAAAAAGCA
    CTAAGAAAAT GTCCAGAGCA AGGAAAAATC CAGGTGATGG AACAGCATTT CCAGGACACT
    GTGCCATTAA TGGTGGATTA TTGCCTTCTA TTACAGCGCA CGGATATCCT TTTTAGCCAG
    ATGTATGACA AGTTAAGTGA GAACTGTGTG GCCAAAGGTG TTTTCTTGGA GTGCTTAGAA
    CCATACCTGT TACGGGACCA GCTGCCAGGG CTGACTGCAC ATGTCATGAA GGATCTGCTC
    CTCCATTTCC AGGACCAAGG GCAGATGGAA AGTGTTGAGG CTTGTATTGT GCACATGGAC
    ATCACCAGCC TGGACATACA AAATATTGTC CACATGTGCT GGGAACATAG ACTTTATGAT
    GCAGTGATCT ATGTGTACAA CAAAGGAACA AATGATTTTA TTAGTCCAAT GGAGAAACTA
    TTTAATGCCA TCTCTGCACC TATCAACTCT GGGAAGTCAC TAACTGATGA GCAAGTTGTG
    ATGGGAAACA AGCTGCTGGT TTACATCAGT TCCAGTTTGG CTGGAAGAGC ATATCCTTTG
    GGGGATATTC CAGAAGACCT TGTGCCTTTG GTGAAAAATC AGGTTTTTGA GTTCCTGATC
    CGTTTGCACT CTCCAGAGAC CCCACAAGAA GAGGAGGTGT ATCCATATAT ACGTACCCTG
    CTGCATTTTG ATACACGGGA GTTCCTCAAT GTACTAGCCT TGACATTTGA AGACTTTACA
    AATGATAAGC AAGCAGTGGA ACATCAGCAA CGTATTGTGG ATATTCTTCT GAAGGTCATG
    GTGGAAAACT CTGACTTTAC TCCATCCCAA GTCGGCTGCC TCTTTACTTT CCTAGCCCGA
    CAGCTTGCCA AGCCAGATAA TACACTTTTT GTGAACCGAA TGTTGTTTGA CCAGGTTTTA
    GAATTCCTGT GTAGTCCAGA TGATGATTCC CGACATTCTG AACGACAGCA GGTTCTCCTT
    GAACTTCTTC ATGCTGGAGG AGTTGTGCAA TTTGAAGAGA ACAGACTGAT CCAGCTAGCA
    GAAAGAGCAC AATTCTATCA GATCTGTGAG TTTATGTATG AGCGGAAACA CCAGTATGAT
    AAAATCATAG ACTGCTACAT GCAAGACCCC ATACGGAAGC AAGATGTATT CAACTACATT
    CATAACATCT TATCAATCCC TGGTTATAGT GCTGAGGAGA AGGAAGCCAC ATGGGACAAA
    ACCATGCAGC ACATGGAGGA ACTAATATTG TTATGCCCTA GTAAAGCAGC TGACCTTGTA
    TGTACACACT TTAGCAGCCA TTTGGAAGAT GTCATTGGAA AGCTCAAGGA TCCGTTATTG
    GTTTTACAGT TCCTTAGAAG TCTCCTTGAT CCAAGGGATG GAGCCCATGT GACTCCTGAA
    GTATTACAAA CTCTTCCACA CATCACAGAG CTTTTCATAG ATTTGATGTA TCAAAATGAT
    CCAGAACAGG TGGCTCCACT GCTGCAGATC CTGGAACACT ATAGACTTGA GGAGACGATA
    CAGATTGCAC AAAGACACAA TCATCATGAA ACCTTGGCAT ATCTCCTGGA GAAGAAAGGG
    GATGTTGAAG CTGCTTTCCG AGTCATGCTT ATGAGACTGC AAGAAAGGCT CTCCAATCTG
    ACCTCAGATT CTGTGGACTC ACCAAGCTTC AAAGTAATGC CCAACATGAA AGATTTAGAA
    GAAACCTTGA CAGAGACCAT TTTACTGTGT GAAAGAACTT CAGACACCCT AAACCAGCAA
    CAGCGAGAGG CATTATGGTT TCCTTTGCTA GAAGCAATGA TGTCTCCGCA AAAGCACTTC
    ACTTCATCTT CTGCACAAAT GTACTCCGAA TCTTTAAAAA CTCTGACTAT GCAAGTTCTG
    AACAGCATGG CCACGTACAT CTCTCTTCCG TCAATCCTCC AAAGAATCCT TCAGGACCCT
    GTATATGGGA AAGGTAAACT AGTTGAAATC CAAGGTCTGG TTTTGGGAAT GCTAGACACA
    TTCAACTATG AACAGACCCT GCTGGAAACT ACTACTAATC TGCTGAACCA AGACCTGCAC
    TGGTCTCTTT GCAACTTGAA GGCTTCAGTC ACCCGGGCTC TTGTTCCCAA ACAGGACTAC
    TGCTGTGTGT GTCTGCAGCA ATACAAGAGG AGACAGGAAG CCACTGATGA AATCATTGTA
    TTCAGCTGTG GCCACCTTTA CCATTCCCTT TGCCTCCTGG GCAAGGACTG CAAGGTTGAA
    GTTGGAGGAC AGAGTCAGTG GATCTGCCAT CGGTGTAACT CAACTAACAA AGGCCGTGCG
    GGGAGGCAGA CAGTGACAGA TGTGAAGAAA GGCCGAACTA CCTCATTAGC GCAGGTGAGA
    GGGACTTCAT CTCGATCATC AAGAGAGGAG GCTGCCAAGA AGTCGAGCAC TGATCCCATT
    CTAGATCCAC AGCAGCTGCA GGCGTTCCAG CATCTCTGCC GTCTCTACAA AGGAACCTCA
    CGGTTGGCTC TCCTTACTGA GCTATGCCGC TCTCAAAGCC CAGAAAAATC ATCTTCATAT
    TATGCTTCCT ATGGTGGAAT TGCTACAAGT AGTGTTTTCC AGAATGAGAA CTTCCAACTG
    CACCTTGCCC CACCCCCTTA CCTTGGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30971
    Clone ID Related Accession (Same CDS sequence) XM_012970874.3 , XM_012970874.2
    Accession Version XM_012970874.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT 
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826328.1
    CDS235..4521
    Translation

    Target ORF information:

    RefSeq Version XM_012970874.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis VPS8, CORVET complex subunit (vps8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012970874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGCCGG AACTAGGAGA GGAAGATGTT GGTTCCAAAG CCTGGGAGCC TGGGATGCTT 
    GCTAGACAAT CTGGTGTCAG CATGGATGAT GATCTAAACA GATCTCAGAG TCGTTTGGGC
    TTCCTAGATA TGGACAAGGA GGCAAAGCAA GACCTGATAG ACGACAAAGA GTTTGACATT
    CCCCAGGTGG ACACCCCCCC TACTCTTGAG AGCATCTTGA ATGAGACAGA TGATGAGGAG
    GAGTCTTTCA TTCCTGAGGA TCCTGCTCTT CTCAATGTAG AGGCAATAGA TGTCCATTCC
    TGTGACACCT CGTCCTTAGC AAGCTCAGAC AGTGGTGATC GGGTACACAA GAGAAAAAAG
    AAACTTCCTG ATGCCTTTTC TCGCCATGGG TCTGTCATGC ACCATTCACT GCTCAAAGGG
    ATCTCTGCTC AAATTGTTTC TGCAGCTGAT AAAGTGGATG CTGGTTTGCC TACTGCTATA
    GCAGTGTCTA ATCTAATTGC AGTTGGTACT TCCCATGGCC TGGCCTTAAT ATTTGACACC
    AATCAGGCCA TGAGGCTCTG CCTGGGGAGC ACAGCAGTAG GGGCACAATA TGGAGCTGTC
    TCTGCTCTCA GTATCAACAG TGACTGCTCC AGATTACTCT GTGGCTTTGC CAAAGGACAG
    ATTACCATGT GGGACCTGGC TGGTGGTAAA CTTTTAAGAA CCATAACAGA TGCCCACCCT
    CCTGGAACTG CAATTTTACA TATCAAGTTC ACAGATGACC CAACTCTTGC AGTCTGCAAT
    GATAGTGGAG GATCCGTATT CGAGTTAACT TTTAAGAGAG TAATGGGAGT GCGAACCTGT
    GAGTCCCGTT GCCTTTTCAG TGGATCCAAG GGAGAAGTTT GCTGTATTGA ACCTCTGCAT
    ACCAAAGCTG AGCTACGCGA TCACCCAATA ACCCACTACT CATTGCTGGC CATGGCATCT
    CTAACCAAGA TTCTTGTCAT TGGCTTGAAA CCCTCTCTGA AAGTCTGGAT GACCTTTCCC
    TATGGACGGA TGGATCCTTC CAGCGTCCCA CTGCTGGCCT GGCACTTTGT TATCTTGCAG
    GGAGCCCTAA ACCCCATGCT TGCATTTTGC AGGGGGGATG TTGTACATTT CCTCTTAGTA
    AAAAGGGATG ATTCTGGAGC TATTCATATC AATAAACAGA AACAACTGCA TTTGCACTAT
    GACCTTATCA ACTTCACTTG GATCAATGCT CGCACGGTGG CTCTTCTCGA CAGCGCAGAA
    AAGCTACACG TAATCGATCG TCAGACACAG GAAGAACTTG AGACCCTGGA GATATCAGAT
    GTGCAACTTG TATACAACAG CAGCCACTTT AAGTCTCTGG CCACTGGAGG AAATGTGAGC
    CAGGCCCTGG CCTTTGTGGG AGAGAAAGCA TGCTATGAAT CAATTAGCAG CTATGGAGGA
    CAGATCTTCT ACCTGGGAAC AAAGTCAGTC CATGCCATGA CGCTGAGAAA CTGGCGAGAG
    AGAGTTGACC ATTTACTGAA GCAGGACTGT TATAATGAAG CTTTGGCATT AGCATGGTCT
    TTTCATGAGG GCAAAGCTAA AGCTGTTGTG GGGCTGTCTG GAGACCCAAA GAAGAGGAAG
    AGTGTTGTAG CTGATAAGAT TGTTGAAATC TTACTCCAGT ATACTGAAAA AGCACTAAGA
    AAATGTCCAG AGCAAGGAAA AATCCAGGTG ATGGAACAGC ATTTCCAGGA CACTGTGCCA
    TTAATGGTGG ATTATTGCCT TCTATTACAG CGCACGGATA TCCTTTTTAG CCAGATGTAT
    GACAAGTTAA GTGAGAACTG TGTGGCCAAA GGTGTTTTCT TGGAGTGCTT AGAACCATAC
    CTGTTACGGG ACCAGCTGCC AGGGCTGACT GCACATGTCA TGAAGGATCT GCTCCTCCAT
    TTCCAGGACC AAGGGCAGAT GGAAAGTGTT GAGGCTTGTA TTGTGCACAT GGACATCACC
    AGCCTGGACA TACAAAATAT TGTCCACATG TGCTGGGAAC ATAGACTTTA TGATGCAGTG
    ATCTATGTGT ACAACAAAGG AACAAATGAT TTTATTAGTC CAATGGAGAA ACTATTTAAT
    GCCATCTCTG CACCTATCAA CTCTGGGAAG TCACTAACTG ATGAGCAAGT TGTGATGGGA
    AACAAGCTGC TGGTTTACAT CAGTTCCAGT TTGGCTGGAA GAGCATATCC TTTGGGGGAT
    ATTCCAGAAG ACCTTGTGCC TTTGGTGAAA AATCAGGTTT TTGAGTTCCT GATCCGTTTG
    CACTCTCCAG AGACCCCACA AGAAGAGGAG GTGTATCCAT ATATACGTAC CCTGCTGCAT
    TTTGATACAC GGGAGTTCCT CAATGTACTA GCCTTGACAT TTGAAGACTT TACAAATGAT
    AAGCAAGCAG TGGAACATCA GCAACGTATT GTGGATATTC TTCTGAAGGT CATGGTGGAA
    AACTCTGACT TTACTCCATC CCAAGTCGGC TGCCTCTTTA CTTTCCTAGC CCGACAGCTT
    GCCAAGCCAG ATAATACACT TTTTGTGAAC CGAATGTTGT TTGACCAGGT TTTAGAATTC
    CTGTGTAGTC CAGATGATGA TTCCCGACAT TCTGAACGAC AGCAGGTTCT CCTTGAACTT
    CTTCATGCTG GAGGAGTTGT GCAATTTGAA GAGAACAGAC TGATCCAGCT AGCAGAAAGA
    GCACAATTCT ATCAGATCTG TGAGTTTATG TATGAGCGGA AACACCAGTA TGATAAAATC
    ATAGACTGCT ACATGCAAGA CCCCATACGG AAGCAAGATG TATTCAACTA CATTCATAAC
    ATCTTATCAA TCCCTGGTTA TAGTGCTGAG GAGAAGGAAG CCACATGGGA CAAAACCATG
    CAGCACATGG AGGAACTAAT ATTGTTATGC CCTAGTAAAG CAGCTGACCT TGTATGTACA
    CACTTTAGCA GCCATTTGGA AGATGTCATT GGAAAGCTCA AGGATCCGTT ATTGGTTTTA
    CAGTTCCTTA GAAGTCTCCT TGATCCAAGG GATGGAGCCC ATGTGACTCC TGAAGTATTA
    CAAACTCTTC CACACATCAC AGAGCTTTTC ATAGATTTGA TGTATCAAAA TGATCCAGAA
    CAGGTGGCTC CACTGCTGCA GATCCTGGAA CACTATAGAC TTGAGGAGAC GATACAGATT
    GCACAAAGAC ACAATCATCA TGAAACCTTG GCATATCTCC TGGAGAAGAA AGGGGATGTT
    GAAGCTGCTT TCCGAGTCAT GCTTATGAGA CTGCAAGAAA GGCTCTCCAA TCTGACCTCA
    GATTCTGTGG ACTCACCAAG CTTCAAAGTA ATGCCCAACA TGAAAGATTT AGAAGAAACC
    TTGACAGAGA CCATTTTACT GTGTGAAAGA ACTTCAGACA CCCTAAACCA GCAACAGCGA
    GAGGCATTAT GGTTTCCTTT GCTAGAAGCA ATGATGTCTC CGCAAAAGCA CTTCACTTCA
    TCTTCTGCAC AAATGTACTC CGAATCTTTA AAAACTCTGA CTATGCAAGT TCTGAACAGC
    ATGGCCACGT ACATCTCTCT TCCGTCAATC CTCCAAAGAA TCCTTCAGGA CCCTGTATAT
    GGGAAAGGTA AACTAGTTGA AATCCAAGGT CTGGTTTTGG GAATGCTAGA CACATTCAAC
    TATGAACAGA CCCTGCTGGA AACTACTACT AATCTGCTGA ACCAAGACCT GCACTGGTCT
    CTTTGCAACT TGAAGGCTTC AGTCACCCGG GCTCTTGTTC CCAAACAGGA CTACTGCTGT
    GTGTGTCTGC AGCAATACAA GAGGAGACAG GAAGCCACTG ATGAAATCAT TGTATTCAGC
    TGTGGCCACC TTTACCATTC CCTTTGCCTC CTGGGCAAGG ACTGCAAGGT TGAAGTTGGA
    GGACAGAGTC AGTGGATCTG CCATCGGTGT AACTCAACTA ACAAAGGCCG TGCGGGGAGG
    CAGACAGTGA CAGATGTGAA GAAAGGCCGA ACTACCTCAT TAGCGCAGGT GAGAGGGACT
    TCATCTCGAT CATCAAGAGA GGAGGCTGCC AAGAAGTCGA GCACTGATCC CATTCTAGAT
    CCACAGCAGC TGCAGGCGTT CCAGCATCTC TGCCGTCTCT ACAAAGGAAC CTCACGGTTG
    GCTCTCCTTA CTGAGCTATG CCGCTCTCAA AGCCCAGAAA AATCATCTTC ATATTATGCT
    TCCTATGGTG GAATTGCTAC AAGTAGTGTT TTCCAGAATG AGAACTTCCA ACTGCACCTT
    GCCCCACCCC CTTACCTTGG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.