VPS8 cDNA ORF clone, Canis lupus familiaris(dog)

The following VPS8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284482 XM_022414314.1
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe284481 XM_022414312.1
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe30481 XM_022414310.1
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30481 XM_005639790.3
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30482 XM_005639791.3
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30483 XM_022414311.1
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30483 XM_005639792.2
Latest version!
Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30484 XM_005639793.2
Latest version!
Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30484 XM_022414313.1
Latest version!
Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe30482 XM_005639791.2 Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe30481 XM_005639790.2 Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284482
    Clone ID Related Accession (Same CDS sequence) XM_022414314.1
    Accession Version XM_022414314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGTACCTG TCATAGTTGA TTACTGCCTG CTGTTGCAAC GAAAGGATCT TTTATTTAGC 
    CAGATGTATG ATAAACTAAG TGAGAACTCA GTGGCCAAAG GAGTGTTTTT GGAGTGCCTT
    GAGCCATATA TTTTAAGTGA TAAATTGGTG GGGATGACCC CCCAGGTAAT GAAAGACTTG
    ATTGTTCATT TCCAAGATAA AAAGTTGATG GAAAATGTGG AAGCCCTCAT TGTACATATG
    GATATCACCA GCCTCGATAT TCAGCAGGTA GTTCTCATGT GTTGGGAAAA TCGTCTGTAT
    GATGCCATGA TCTATGTCTA CAACAGAGGC ATGAATGAAT TTATTAGTCC AATGGAGAAA
    CTTTTCAGAG TCATTGCTCC TCCTCTGAAT GCAGGAAGGA CACTAACAGA TGAGCAAGTT
    GTTATGGGCA ACAAGCTTCT CGTATATATT AGCTGTTGTC TTGCAGGCCG AGCCTATCCT
    CTTGGTGACA TCCCTGAAGA TCTTGTTCCC TTGGTTAAAA ACCAGGTTTT TGAATTTCTG
    ATTCGCCTGC ATTCAGCAGA AGCTTCCCCT GAGGAAGAAA TCTACCCCTA TGTTCGAACT
    CTGCTACACT TTGACACAAG AGAATTTCTA AATGTTTTGG CACTGACTTT TGAAGATTTT
    AAAAATGACA AGCAAGCTGT AGAATATCAA CAACGAATTG TGGATATTTT GTTGAAAGTT
    ATGGTGGAGA ATTCTGATTT TACTCCCTCA CAAGTAGGGT GTCTCTTTAC ATTCCTTGCC
    CGGCAGCTTG CAAAGCCTGA CAATACCTTA TTTGTAAACA GAACCCTTTT TGATCAGGTC
    CTTGAATTCC TCTGCAGTCC TGATGATGAC TCCCGACACT CTGAAAGACA GCAGGTCCTT
    TTAGAGTTGC TGCAAGCTGG AGGCATAGTT CAGTTTGAAG AGAGTCGGCT TATCCGGATG
    GCAGAAAAAG CAGAATTCTA TCAAATTTGT GAGTTTATGT ATGAACGAGA ACACCAATAT
    GACAAAATAA TCGATTGCTA CTTACGTGAC CCACTAAGGG AGGAAGAAGT CTTTAACTAT
    ATTCACAATA TATTATCCAT TCCTGGACAC AGTGCAGAGG AGAAGCAATC TGTATGGCAG
    AAAGCAATGG ATCATATCGA GGAACTTGTA TCCCTGAGGC CCTGTAAAGC TGCAGAGCTG
    GTTGCTACTC ACTTTTCTGA ACAGATTGAA ACAGTCATTA AAAAACTCCA GAACCAAGTT
    TTGCTTTTCA AATTTTTGAG GAGTCTTCTT GACCCAAGGG AAGGTATTCA TGTAAATCAA
    GAATTGCTGC AGATGTCTCC CTGTGTTACG GAGCAGTTCA TTGAGCTGCT ATGTCAGTTC
    AGCCCAAACC AGGTTATAGA GACACTGCAG GTCCTTGAGT GCTACCGCCT GGAAGAAACC
    ATCCAGATTA CCCAAAAGTA TCAACTTCAT GAAGTCACTG CCTATCTATT AGAAAAGAAA
    GGAGATATTC ATGGTGCCTT CTTAATAATG CTAAAGAGGC TACAGAGCAA ACTTCAAGAA
    ATAACACATC AAGATGAAAA ATCCAAAGAA GATCTCTCAC TGAAGGATGT TGAAGATACC
    ATGGTAGAGA CAATTGCCCT TTGCCAGAGA AATTCACATA ATTTGAACCA GCAGCAACGA
    GAGGCACTCT GGTTTCCATT ATTGGAGGCA ATGATGGCTC CTCAGAAGCT GTCTGGTTCA
    GCTGCGCCTC ATCTACACCC TGAAGCTCTG AAATCTTTGA CCATGCAAGT TCTAAACAGC
    ATGGCAGCAT TTATTGCTCT TCCATCAATC TTGCAAAGAA TCTTACAGGA TCCAGTTTAT
    GGAAAAGGAA AACTTGGAGA AATCCAGGGA CTTATTCTGG GAATGTTGGA TACCTTTAAT
    TATGAACAAA CCCTGCTGGA AACAACTACT AGCCTTCTAA ACCAAGATCT CCATTGGTCA
    TTGTGTAACC TGAGAGCTTC AGTCACCAGA GGACTGAATC CCAAGCAGGA TTACTGCTCT
    ATATGTTTGC AGCAATACAA GAGACGCCAA GAAATGGCTG ATGAAATCAT TGTCTTTAGC
    TGTGGCCATT TGTATCACTC ATTCTGTCTG CAAAACAAAG AGTGCACCAT AGAAACTGAG
    GGGCAAACAA GATGGACATG CTACAAATGC AGCTCAAGTA ACAAAGGAGG GAAACTAAGT
    GAAAATTCAT CTGAAATTAA AAAGGGAAAG ACAACCTCAT CACAGGTAAA AATGTCTCCT
    TCTTGTCATC CATCCAAAGG AGATTCTACT GTTAAGAAGG CAGCCTCAGA ACCCGTTCTG
    GATCCACAGC AAATCCAAGC ATTTGATCAG CTCTGCCGTC TCTACCGAGG AAGTTCCCGG
    CTGGCTCTCC TCACAGAACT CTCCCAGAAT CGCAGCAGTG AGAGCTACAG GCCATTCAGT
    GGCTCCCAGA GTGGCTCTGC TTTCAGCAGC ATCTTCCAGA ATGAGAATTT CCAGCTGCAG
    CTCATTCCCC CACCTGTGAC TGAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270022.1
    CDS81..2630
    Translation

    Target ORF information:

    RefSeq Version XM_022414314.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGTACCTG TCATAGTTGA TTACTGCCTG CTGTTGCAAC GAAAGGATCT TTTATTTAGC 
    CAGATGTATG ATAAACTAAG TGAGAACTCA GTGGCCAAAG GAGTGTTTTT GGAGTGCCTT
    GAGCCATATA TTTTAAGTGA TAAATTGGTG GGGATGACCC CCCAGGTAAT GAAAGACTTG
    ATTGTTCATT TCCAAGATAA AAAGTTGATG GAAAATGTGG AAGCCCTCAT TGTACATATG
    GATATCACCA GCCTCGATAT TCAGCAGGTA GTTCTCATGT GTTGGGAAAA TCGTCTGTAT
    GATGCCATGA TCTATGTCTA CAACAGAGGC ATGAATGAAT TTATTAGTCC AATGGAGAAA
    CTTTTCAGAG TCATTGCTCC TCCTCTGAAT GCAGGAAGGA CACTAACAGA TGAGCAAGTT
    GTTATGGGCA ACAAGCTTCT CGTATATATT AGCTGTTGTC TTGCAGGCCG AGCCTATCCT
    CTTGGTGACA TCCCTGAAGA TCTTGTTCCC TTGGTTAAAA ACCAGGTTTT TGAATTTCTG
    ATTCGCCTGC ATTCAGCAGA AGCTTCCCCT GAGGAAGAAA TCTACCCCTA TGTTCGAACT
    CTGCTACACT TTGACACAAG AGAATTTCTA AATGTTTTGG CACTGACTTT TGAAGATTTT
    AAAAATGACA AGCAAGCTGT AGAATATCAA CAACGAATTG TGGATATTTT GTTGAAAGTT
    ATGGTGGAGA ATTCTGATTT TACTCCCTCA CAAGTAGGGT GTCTCTTTAC ATTCCTTGCC
    CGGCAGCTTG CAAAGCCTGA CAATACCTTA TTTGTAAACA GAACCCTTTT TGATCAGGTC
    CTTGAATTCC TCTGCAGTCC TGATGATGAC TCCCGACACT CTGAAAGACA GCAGGTCCTT
    TTAGAGTTGC TGCAAGCTGG AGGCATAGTT CAGTTTGAAG AGAGTCGGCT TATCCGGATG
    GCAGAAAAAG CAGAATTCTA TCAAATTTGT GAGTTTATGT ATGAACGAGA ACACCAATAT
    GACAAAATAA TCGATTGCTA CTTACGTGAC CCACTAAGGG AGGAAGAAGT CTTTAACTAT
    ATTCACAATA TATTATCCAT TCCTGGACAC AGTGCAGAGG AGAAGCAATC TGTATGGCAG
    AAAGCAATGG ATCATATCGA GGAACTTGTA TCCCTGAGGC CCTGTAAAGC TGCAGAGCTG
    GTTGCTACTC ACTTTTCTGA ACAGATTGAA ACAGTCATTA AAAAACTCCA GAACCAAGTT
    TTGCTTTTCA AATTTTTGAG GAGTCTTCTT GACCCAAGGG AAGGTATTCA TGTAAATCAA
    GAATTGCTGC AGATGTCTCC CTGTGTTACG GAGCAGTTCA TTGAGCTGCT ATGTCAGTTC
    AGCCCAAACC AGGTTATAGA GACACTGCAG GTCCTTGAGT GCTACCGCCT GGAAGAAACC
    ATCCAGATTA CCCAAAAGTA TCAACTTCAT GAAGTCACTG CCTATCTATT AGAAAAGAAA
    GGAGATATTC ATGGTGCCTT CTTAATAATG CTAAAGAGGC TACAGAGCAA ACTTCAAGAA
    ATAACACATC AAGATGAAAA ATCCAAAGAA GATCTCTCAC TGAAGGATGT TGAAGATACC
    ATGGTAGAGA CAATTGCCCT TTGCCAGAGA AATTCACATA ATTTGAACCA GCAGCAACGA
    GAGGCACTCT GGTTTCCATT ATTGGAGGCA ATGATGGCTC CTCAGAAGCT GTCTGGTTCA
    GCTGCGCCTC ATCTACACCC TGAAGCTCTG AAATCTTTGA CCATGCAAGT TCTAAACAGC
    ATGGCAGCAT TTATTGCTCT TCCATCAATC TTGCAAAGAA TCTTACAGGA TCCAGTTTAT
    GGAAAAGGAA AACTTGGAGA AATCCAGGGA CTTATTCTGG GAATGTTGGA TACCTTTAAT
    TATGAACAAA CCCTGCTGGA AACAACTACT AGCCTTCTAA ACCAAGATCT CCATTGGTCA
    TTGTGTAACC TGAGAGCTTC AGTCACCAGA GGACTGAATC CCAAGCAGGA TTACTGCTCT
    ATATGTTTGC AGCAATACAA GAGACGCCAA GAAATGGCTG ATGAAATCAT TGTCTTTAGC
    TGTGGCCATT TGTATCACTC ATTCTGTCTG CAAAACAAAG AGTGCACCAT AGAAACTGAG
    GGGCAAACAA GATGGACATG CTACAAATGC AGCTCAAGTA ACAAAGGAGG GAAACTAAGT
    GAAAATTCAT CTGAAATTAA AAAGGGAAAG ACAACCTCAT CACAGGTAAA AATGTCTCCT
    TCTTGTCATC CATCCAAAGG AGATTCTACT GTTAAGAAGG CAGCCTCAGA ACCCGTTCTG
    GATCCACAGC AAATCCAAGC ATTTGATCAG CTCTGCCGTC TCTACCGAGG AAGTTCCCGG
    CTGGCTCTCC TCACAGAACT CTCCCAGAAT CGCAGCAGTG AGAGCTACAG GCCATTCAGT
    GGCTCCCAGA GTGGCTCTGC TTTCAGCAGC ATCTTCCAGA ATGAGAATTT CCAGCTGCAG
    CTCATTCCCC CACCTGTGAC TGAGGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe284481
    Clone ID Related Accession (Same CDS sequence) XM_022414312.1
    Accession Version XM_022414312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGCTGT GCTCAAAGAA AACTGGCCCA
    AGATATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270020.1
    CDS1..4089
    Translation

    Target ORF information:

    RefSeq Version XM_022414312.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGCTGT GCTCAAAGAA AACTGGCCCA
    AGATATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30481
    Clone ID Related Accession (Same CDS sequence) XM_005639790.2 , XM_022414310.1 , XM_005639790.3
    Accession Version XM_005639790.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGGCAG CCTCAGAACC CGTTCTGGAT
    CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCGTCTCT ACCGAGGAAG TTCCCGGCTG
    GCTCTCCTCA CAGAACTCTC CCAGAATCGC AGCAGTGAGA GCTACAGGCC ATTCAGTGGC
    TCCCAGAGTG GCTCTGCTTT CAGCAGCATC TTCCAGAATG AGAATTTCCA GCTGCAGCTC
    ATTCCCCCAC CTGTGACTGA GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639847.1
    CDS177..4463
    Translation

    Target ORF information:

    RefSeq Version XM_005639790.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639790.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGGCAG CCTCAGAACC CGTTCTGGAT
    CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCGTCTCT ACCGAGGAAG TTCCCGGCTG
    GCTCTCCTCA CAGAACTCTC CCAGAATCGC AGCAGTGAGA GCTACAGGCC ATTCAGTGGC
    TCCCAGAGTG GCTCTGCTTT CAGCAGCATC TTCCAGAATG AGAATTTCCA GCTGCAGCTC
    ATTCCCCCAC CTGTGACTGA GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30481
    Clone ID Related Accession (Same CDS sequence) XM_005639790.2 , XM_022414310.1 , XM_005639790.3
    Accession Version XM_022414310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGGCAG CCTCAGAACC CGTTCTGGAT
    CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCGTCTCT ACCGAGGAAG TTCCCGGCTG
    GCTCTCCTCA CAGAACTCTC CCAGAATCGC AGCAGTGAGA GCTACAGGCC ATTCAGTGGC
    TCCCAGAGTG GCTCTGCTTT CAGCAGCATC TTCCAGAATG AGAATTTCCA GCTGCAGCTC
    ATTCCCCCAC CTGTGACTGA GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270018.1
    CDS1..4287
    Translation

    Target ORF information:

    RefSeq Version XM_022414310.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGGCAG CCTCAGAACC CGTTCTGGAT
    CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCGTCTCT ACCGAGGAAG TTCCCGGCTG
    GCTCTCCTCA CAGAACTCTC CCAGAATCGC AGCAGTGAGA GCTACAGGCC ATTCAGTGGC
    TCCCAGAGTG GCTCTGCTTT CAGCAGCATC TTCCAGAATG AGAATTTCCA GCTGCAGCTC
    ATTCCCCCAC CTGTGACTGA GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30481
    Clone ID Related Accession (Same CDS sequence) XM_005639790.2 , XM_022414310.1 , XM_005639790.3
    Accession Version XM_005639790.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGGCAG CCTCAGAACC CGTTCTGGAT
    CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCGTCTCT ACCGAGGAAG TTCCCGGCTG
    GCTCTCCTCA CAGAACTCTC CCAGAATCGC AGCAGTGAGA GCTACAGGCC ATTCAGTGGC
    TCCCAGAGTG GCTCTGCTTT CAGCAGCATC TTCCAGAATG AGAATTTCCA GCTGCAGCTC
    ATTCCCCCAC CTGTGACTGA GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639847.1
    CDS211..4497
    Translation

    Target ORF information:

    RefSeq Version XM_005639790.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639790.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGGCAG CCTCAGAACC CGTTCTGGAT
    CCACAGCAAA TCCAAGCATT TGATCAGCTC TGCCGTCTCT ACCGAGGAAG TTCCCGGCTG
    GCTCTCCTCA CAGAACTCTC CCAGAATCGC AGCAGTGAGA GCTACAGGCC ATTCAGTGGC
    TCCCAGAGTG GCTCTGCTTT CAGCAGCATC TTCCAGAATG AGAATTTCCA GCTGCAGCTC
    ATTCCCCCAC CTGTGACTGA GGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30482
    Clone ID Related Accession (Same CDS sequence) XM_005639791.3 , XM_005639791.2
    Accession Version XM_005639791.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGATCAG
    AATCAAGCTT TGCGACTCTG TCTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCCATC
    TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC AAAAGGACAG
    ATCACGATGT GGGATTTGGC CAGTGGAAAA CTTCTAAGAT CAATAACAGA TGCTCATCCT
    CCAGGAACAG CTATACTGCA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT
    GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACATGT
    GAATCTAGAT GTCTGTTCAG TGGTTCCAAG GGTGAAGTTT GCTGTATTGA GCCTCTCCAT
    TCAAAGCCTG AGTTAAAAGA TCATCCCATC ACACAGTTTT CCTTATTGGC CATGGCGTCC
    TTAACAAAGA TACTGGTCAT TGGATTGAAA CCATCTTTGA AAGTGTGGAT GACTTTTCCC
    TATGGACGGA TGGATCCTTC CAGTGTTCCA TTGCTGGCCT GGCACTTTGT GGCAGTGCAT
    AATTATGTGA ATCCCATGCT TGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTGTTGGTT
    AAGAGAGATG AGTCTGGAGC AATACATGTT ACTAAGCAAA AGCACCTTCA TCTCTACTAT
    GATCTCATCA ACTTTACTTG GATAAACTCA CGCACAGTTG TGCTCTTGGA CAGTGTGGAG
    AAGTTGCATG TGATTGATCG GCAGACTCAA GAGGAATTGG AAACAGTGGA GATCTCAGAG
    GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCCCTAG CCACCGGAGG AAATGTTAGC
    CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGTTATCAAT CCATCAGTAG CTATGGTGGT
    CAGATCTTTT ATTTGGGGAC AAAATCTGTT TATGTGATGA TGCTGAGAAG CTGGAGAGAG
    AGAGTGGATC ATCTCCTGAA ACAAGATTGT CTTACAGAAG CCTTGGCTCT TGCATGGTCT
    TTCCATGAAG GAAAAGCAAA AGCAGTAGTG GGATTATCAG GGGATGCCAG TAAGCGAAAG
    GCTGTTGTTG CAGATCGGAT GGTAGAAATC CTGTTCCACT ATGCAGATCG AGCTCTGAAA
    AAGTGTCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAGC ATTTTCAGGA CATGGTACCT
    GTCATAGTTG ATTACTGCCT GCTGTTGCAA CGAAAGGATC TTTTATTTAG CCAGATGTAT
    GATAAACTAA GTGAGAACTC AGTGGCCAAA GGAGTGTTTT TGGAGTGCCT TGAGCCATAT
    ATTTTAAGTG ATAAATTGGT GGGGATGACC CCCCAGGTAA TGAAAGACTT GATTGTTCAT
    TTCCAAGATA AAAAGTTGAT GGAAAATGTG GAAGCCCTCA TTGTACATAT GGATATCACC
    AGCCTCGATA TTCAGCAGGT AGTTCTCATG TGTTGGGAAA ATCGTCTGTA TGATGCCATG
    ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA
    GTCATTGCTC CTCCTCTGAA TGCAGGAAGG ACACTAACAG ATGAGCAAGT TGTTATGGGC
    AACAAGCTTC TCGTATATAT TAGCTGTTGT CTTGCAGGCC GAGCCTATCC TCTTGGTGAC
    ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT GATTCGCCTG
    CATTCAGCAG AAGCTTCCCC TGAGGAAGAA ATCTACCCCT ATGTTCGAAC TCTGCTACAC
    TTTGACACAA GAGAATTTCT AAATGTTTTG GCACTGACTT TTGAAGATTT TAAAAATGAC
    AAGCAAGCTG TAGAATATCA ACAACGAATT GTGGATATTT TGTTGAAAGT TATGGTGGAG
    AATTCTGATT TTACTCCCTC ACAAGTAGGG TGTCTCTTTA CATTCCTTGC CCGGCAGCTT
    GCAAAGCCTG ACAATACCTT ATTTGTAAAC AGAACCCTTT TTGATCAGGT CCTTGAATTC
    CTCTGCAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAGTTG
    CTGCAAGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGGC TTATCCGGAT GGCAGAAAAA
    GCAGAATTCT ATCAAATTTG TGAGTTTATG TATGAACGAG AACACCAATA TGACAAAATA
    ATCGATTGCT ACTTACGTGA CCCACTAAGG GAGGAAGAAG TCTTTAACTA TATTCACAAT
    ATATTATCCA TTCCTGGACA CAGTGCAGAG GAGAAGCAAT CTGTATGGCA GAAAGCAATG
    GATCATATCG AGGAACTTGT ATCCCTGAGG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT
    CACTTTTCTG AACAGATTGA AACAGTCATT AAAAAACTCC AGAACCAAGT TTTGCTTTTC
    AAATTTTTGA GGAGTCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATTGCTG
    CAGATGTCTC CCTGTGTTAC GGAGCAGTTC ATTGAGCTGC TATGTCAGTT CAGCCCAAAC
    CAGGTTATAG AGACACTGCA GGTCCTTGAG TGCTACCGCC TGGAAGAAAC CATCCAGATT
    ACCCAAAAGT ATCAACTTCA TGAAGTCACT GCCTATCTAT TAGAAAAGAA AGGAGATATT
    CATGGTGCCT TCTTAATAAT GCTAAAGAGG CTACAGAGCA AACTTCAAGA AATAACACAT
    CAAGATGAAA AATCCAAAGA AGATCTCTCA CTGAAGGATG TTGAAGATAC CATGGTAGAG
    ACAATTGCCC TTTGCCAGAG AAATTCACAT AATTTGAACC AGCAGCAACG AGAGGCACTC
    TGGTTTCCAT TATTGGAGGC AATGATGGCT CCTCAGAAGC TGTCTGGTTC AGCTGCGCCT
    CATCTACACC CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTAAACAG CATGGCAGCA
    TTTATTGCTC TTCCATCAAT CTTGCAAAGA ATCTTACAGG ATCCAGTTTA TGGAAAAGGA
    AAACTTGGAG AAATCCAGGG ACTTATTCTG GGAATGTTGG ATACCTTTAA TTATGAACAA
    ACCCTGCTGG AAACAACTAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC
    CTGAGAGCTT CAGTCACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATATGTTTG
    CAGCAATACA AGAGACGCCA AGAAATGGCT GATGAAATCA TTGTCTTTAG CTGTGGCCAT
    TTGTATCACT CATTCTGTCT GCAAAACAAA GAGTGCACCA TAGAAACTGA GGGGCAAACA
    AGATGGACAT GCTACAAATG CAGCTCAAGT AACAAAGGAG GGAAACTAAG TGAAAATTCA
    TCTGAAATTA AAAAGGGAAA GACAACCTCA TCACAGGTAA AAATGTCTCC TTCTTGTCAT
    CCATCCAAAG GAGATTCTAC TGTTAAGAAG GCAGCCTCAG AACCCGTTCT GGATCCACAG
    CAAATCCAAG CATTTGATCA GCTCTGCCGT CTCTACCGAG GAAGTTCCCG GCTGGCTCTC
    CTCACAGAAC TCTCCCAGAA TCGCAGCAGT GAGAGCTACA GGCCATTCAG TGGCTCCCAG
    AGTGGCTCTG CTTTCAGCAG CATCTTCCAG AATGAGAATT TCCAGCTGCA GCTCATTCCC
    CCACCTGTGA CTGAGGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639848.1
    CDS211..4491
    Translation

    Target ORF information:

    RefSeq Version XM_005639791.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639791.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGATCAG
    AATCAAGCTT TGCGACTCTG TCTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCCATC
    TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC AAAAGGACAG
    ATCACGATGT GGGATTTGGC CAGTGGAAAA CTTCTAAGAT CAATAACAGA TGCTCATCCT
    CCAGGAACAG CTATACTGCA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT
    GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACATGT
    GAATCTAGAT GTCTGTTCAG TGGTTCCAAG GGTGAAGTTT GCTGTATTGA GCCTCTCCAT
    TCAAAGCCTG AGTTAAAAGA TCATCCCATC ACACAGTTTT CCTTATTGGC CATGGCGTCC
    TTAACAAAGA TACTGGTCAT TGGATTGAAA CCATCTTTGA AAGTGTGGAT GACTTTTCCC
    TATGGACGGA TGGATCCTTC CAGTGTTCCA TTGCTGGCCT GGCACTTTGT GGCAGTGCAT
    AATTATGTGA ATCCCATGCT TGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTGTTGGTT
    AAGAGAGATG AGTCTGGAGC AATACATGTT ACTAAGCAAA AGCACCTTCA TCTCTACTAT
    GATCTCATCA ACTTTACTTG GATAAACTCA CGCACAGTTG TGCTCTTGGA CAGTGTGGAG
    AAGTTGCATG TGATTGATCG GCAGACTCAA GAGGAATTGG AAACAGTGGA GATCTCAGAG
    GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCCCTAG CCACCGGAGG AAATGTTAGC
    CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGTTATCAAT CCATCAGTAG CTATGGTGGT
    CAGATCTTTT ATTTGGGGAC AAAATCTGTT TATGTGATGA TGCTGAGAAG CTGGAGAGAG
    AGAGTGGATC ATCTCCTGAA ACAAGATTGT CTTACAGAAG CCTTGGCTCT TGCATGGTCT
    TTCCATGAAG GAAAAGCAAA AGCAGTAGTG GGATTATCAG GGGATGCCAG TAAGCGAAAG
    GCTGTTGTTG CAGATCGGAT GGTAGAAATC CTGTTCCACT ATGCAGATCG AGCTCTGAAA
    AAGTGTCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAGC ATTTTCAGGA CATGGTACCT
    GTCATAGTTG ATTACTGCCT GCTGTTGCAA CGAAAGGATC TTTTATTTAG CCAGATGTAT
    GATAAACTAA GTGAGAACTC AGTGGCCAAA GGAGTGTTTT TGGAGTGCCT TGAGCCATAT
    ATTTTAAGTG ATAAATTGGT GGGGATGACC CCCCAGGTAA TGAAAGACTT GATTGTTCAT
    TTCCAAGATA AAAAGTTGAT GGAAAATGTG GAAGCCCTCA TTGTACATAT GGATATCACC
    AGCCTCGATA TTCAGCAGGT AGTTCTCATG TGTTGGGAAA ATCGTCTGTA TGATGCCATG
    ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA
    GTCATTGCTC CTCCTCTGAA TGCAGGAAGG ACACTAACAG ATGAGCAAGT TGTTATGGGC
    AACAAGCTTC TCGTATATAT TAGCTGTTGT CTTGCAGGCC GAGCCTATCC TCTTGGTGAC
    ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT GATTCGCCTG
    CATTCAGCAG AAGCTTCCCC TGAGGAAGAA ATCTACCCCT ATGTTCGAAC TCTGCTACAC
    TTTGACACAA GAGAATTTCT AAATGTTTTG GCACTGACTT TTGAAGATTT TAAAAATGAC
    AAGCAAGCTG TAGAATATCA ACAACGAATT GTGGATATTT TGTTGAAAGT TATGGTGGAG
    AATTCTGATT TTACTCCCTC ACAAGTAGGG TGTCTCTTTA CATTCCTTGC CCGGCAGCTT
    GCAAAGCCTG ACAATACCTT ATTTGTAAAC AGAACCCTTT TTGATCAGGT CCTTGAATTC
    CTCTGCAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAGTTG
    CTGCAAGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGGC TTATCCGGAT GGCAGAAAAA
    GCAGAATTCT ATCAAATTTG TGAGTTTATG TATGAACGAG AACACCAATA TGACAAAATA
    ATCGATTGCT ACTTACGTGA CCCACTAAGG GAGGAAGAAG TCTTTAACTA TATTCACAAT
    ATATTATCCA TTCCTGGACA CAGTGCAGAG GAGAAGCAAT CTGTATGGCA GAAAGCAATG
    GATCATATCG AGGAACTTGT ATCCCTGAGG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT
    CACTTTTCTG AACAGATTGA AACAGTCATT AAAAAACTCC AGAACCAAGT TTTGCTTTTC
    AAATTTTTGA GGAGTCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATTGCTG
    CAGATGTCTC CCTGTGTTAC GGAGCAGTTC ATTGAGCTGC TATGTCAGTT CAGCCCAAAC
    CAGGTTATAG AGACACTGCA GGTCCTTGAG TGCTACCGCC TGGAAGAAAC CATCCAGATT
    ACCCAAAAGT ATCAACTTCA TGAAGTCACT GCCTATCTAT TAGAAAAGAA AGGAGATATT
    CATGGTGCCT TCTTAATAAT GCTAAAGAGG CTACAGAGCA AACTTCAAGA AATAACACAT
    CAAGATGAAA AATCCAAAGA AGATCTCTCA CTGAAGGATG TTGAAGATAC CATGGTAGAG
    ACAATTGCCC TTTGCCAGAG AAATTCACAT AATTTGAACC AGCAGCAACG AGAGGCACTC
    TGGTTTCCAT TATTGGAGGC AATGATGGCT CCTCAGAAGC TGTCTGGTTC AGCTGCGCCT
    CATCTACACC CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTAAACAG CATGGCAGCA
    TTTATTGCTC TTCCATCAAT CTTGCAAAGA ATCTTACAGG ATCCAGTTTA TGGAAAAGGA
    AAACTTGGAG AAATCCAGGG ACTTATTCTG GGAATGTTGG ATACCTTTAA TTATGAACAA
    ACCCTGCTGG AAACAACTAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC
    CTGAGAGCTT CAGTCACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATATGTTTG
    CAGCAATACA AGAGACGCCA AGAAATGGCT GATGAAATCA TTGTCTTTAG CTGTGGCCAT
    TTGTATCACT CATTCTGTCT GCAAAACAAA GAGTGCACCA TAGAAACTGA GGGGCAAACA
    AGATGGACAT GCTACAAATG CAGCTCAAGT AACAAAGGAG GGAAACTAAG TGAAAATTCA
    TCTGAAATTA AAAAGGGAAA GACAACCTCA TCACAGGTAA AAATGTCTCC TTCTTGTCAT
    CCATCCAAAG GAGATTCTAC TGTTAAGAAG GCAGCCTCAG AACCCGTTCT GGATCCACAG
    CAAATCCAAG CATTTGATCA GCTCTGCCGT CTCTACCGAG GAAGTTCCCG GCTGGCTCTC
    CTCACAGAAC TCTCCCAGAA TCGCAGCAGT GAGAGCTACA GGCCATTCAG TGGCTCCCAG
    AGTGGCTCTG CTTTCAGCAG CATCTTCCAG AATGAGAATT TCCAGCTGCA GCTCATTCCC
    CCACCTGTGA CTGAGGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30482
    Clone ID Related Accession (Same CDS sequence) XM_005639791.3 , XM_005639791.2
    Accession Version XM_005639791.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGATCAG
    AATCAAGCTT TGCGACTCTG TCTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCCATC
    TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC AAAAGGACAG
    ATCACGATGT GGGATTTGGC CAGTGGAAAA CTTCTAAGAT CAATAACAGA TGCTCATCCT
    CCAGGAACAG CTATACTGCA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT
    GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACATGT
    GAATCTAGAT GTCTGTTCAG TGGTTCCAAG GGTGAAGTTT GCTGTATTGA GCCTCTCCAT
    TCAAAGCCTG AGTTAAAAGA TCATCCCATC ACACAGTTTT CCTTATTGGC CATGGCGTCC
    TTAACAAAGA TACTGGTCAT TGGATTGAAA CCATCTTTGA AAGTGTGGAT GACTTTTCCC
    TATGGACGGA TGGATCCTTC CAGTGTTCCA TTGCTGGCCT GGCACTTTGT GGCAGTGCAT
    AATTATGTGA ATCCCATGCT TGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTGTTGGTT
    AAGAGAGATG AGTCTGGAGC AATACATGTT ACTAAGCAAA AGCACCTTCA TCTCTACTAT
    GATCTCATCA ACTTTACTTG GATAAACTCA CGCACAGTTG TGCTCTTGGA CAGTGTGGAG
    AAGTTGCATG TGATTGATCG GCAGACTCAA GAGGAATTGG AAACAGTGGA GATCTCAGAG
    GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCCCTAG CCACCGGAGG AAATGTTAGC
    CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGTTATCAAT CCATCAGTAG CTATGGTGGT
    CAGATCTTTT ATTTGGGGAC AAAATCTGTT TATGTGATGA TGCTGAGAAG CTGGAGAGAG
    AGAGTGGATC ATCTCCTGAA ACAAGATTGT CTTACAGAAG CCTTGGCTCT TGCATGGTCT
    TTCCATGAAG GAAAAGCAAA AGCAGTAGTG GGATTATCAG GGGATGCCAG TAAGCGAAAG
    GCTGTTGTTG CAGATCGGAT GGTAGAAATC CTGTTCCACT ATGCAGATCG AGCTCTGAAA
    AAGTGTCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAGC ATTTTCAGGA CATGGTACCT
    GTCATAGTTG ATTACTGCCT GCTGTTGCAA CGAAAGGATC TTTTATTTAG CCAGATGTAT
    GATAAACTAA GTGAGAACTC AGTGGCCAAA GGAGTGTTTT TGGAGTGCCT TGAGCCATAT
    ATTTTAAGTG ATAAATTGGT GGGGATGACC CCCCAGGTAA TGAAAGACTT GATTGTTCAT
    TTCCAAGATA AAAAGTTGAT GGAAAATGTG GAAGCCCTCA TTGTACATAT GGATATCACC
    AGCCTCGATA TTCAGCAGGT AGTTCTCATG TGTTGGGAAA ATCGTCTGTA TGATGCCATG
    ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA
    GTCATTGCTC CTCCTCTGAA TGCAGGAAGG ACACTAACAG ATGAGCAAGT TGTTATGGGC
    AACAAGCTTC TCGTATATAT TAGCTGTTGT CTTGCAGGCC GAGCCTATCC TCTTGGTGAC
    ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT GATTCGCCTG
    CATTCAGCAG AAGCTTCCCC TGAGGAAGAA ATCTACCCCT ATGTTCGAAC TCTGCTACAC
    TTTGACACAA GAGAATTTCT AAATGTTTTG GCACTGACTT TTGAAGATTT TAAAAATGAC
    AAGCAAGCTG TAGAATATCA ACAACGAATT GTGGATATTT TGTTGAAAGT TATGGTGGAG
    AATTCTGATT TTACTCCCTC ACAAGTAGGG TGTCTCTTTA CATTCCTTGC CCGGCAGCTT
    GCAAAGCCTG ACAATACCTT ATTTGTAAAC AGAACCCTTT TTGATCAGGT CCTTGAATTC
    CTCTGCAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAGTTG
    CTGCAAGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGGC TTATCCGGAT GGCAGAAAAA
    GCAGAATTCT ATCAAATTTG TGAGTTTATG TATGAACGAG AACACCAATA TGACAAAATA
    ATCGATTGCT ACTTACGTGA CCCACTAAGG GAGGAAGAAG TCTTTAACTA TATTCACAAT
    ATATTATCCA TTCCTGGACA CAGTGCAGAG GAGAAGCAAT CTGTATGGCA GAAAGCAATG
    GATCATATCG AGGAACTTGT ATCCCTGAGG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT
    CACTTTTCTG AACAGATTGA AACAGTCATT AAAAAACTCC AGAACCAAGT TTTGCTTTTC
    AAATTTTTGA GGAGTCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATTGCTG
    CAGATGTCTC CCTGTGTTAC GGAGCAGTTC ATTGAGCTGC TATGTCAGTT CAGCCCAAAC
    CAGGTTATAG AGACACTGCA GGTCCTTGAG TGCTACCGCC TGGAAGAAAC CATCCAGATT
    ACCCAAAAGT ATCAACTTCA TGAAGTCACT GCCTATCTAT TAGAAAAGAA AGGAGATATT
    CATGGTGCCT TCTTAATAAT GCTAAAGAGG CTACAGAGCA AACTTCAAGA AATAACACAT
    CAAGATGAAA AATCCAAAGA AGATCTCTCA CTGAAGGATG TTGAAGATAC CATGGTAGAG
    ACAATTGCCC TTTGCCAGAG AAATTCACAT AATTTGAACC AGCAGCAACG AGAGGCACTC
    TGGTTTCCAT TATTGGAGGC AATGATGGCT CCTCAGAAGC TGTCTGGTTC AGCTGCGCCT
    CATCTACACC CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTAAACAG CATGGCAGCA
    TTTATTGCTC TTCCATCAAT CTTGCAAAGA ATCTTACAGG ATCCAGTTTA TGGAAAAGGA
    AAACTTGGAG AAATCCAGGG ACTTATTCTG GGAATGTTGG ATACCTTTAA TTATGAACAA
    ACCCTGCTGG AAACAACTAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC
    CTGAGAGCTT CAGTCACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATATGTTTG
    CAGCAATACA AGAGACGCCA AGAAATGGCT GATGAAATCA TTGTCTTTAG CTGTGGCCAT
    TTGTATCACT CATTCTGTCT GCAAAACAAA GAGTGCACCA TAGAAACTGA GGGGCAAACA
    AGATGGACAT GCTACAAATG CAGCTCAAGT AACAAAGGAG GGAAACTAAG TGAAAATTCA
    TCTGAAATTA AAAAGGGAAA GACAACCTCA TCACAGGTAA AAATGTCTCC TTCTTGTCAT
    CCATCCAAAG GAGATTCTAC TGTTAAGAAG GCAGCCTCAG AACCCGTTCT GGATCCACAG
    CAAATCCAAG CATTTGATCA GCTCTGCCGT CTCTACCGAG GAAGTTCCCG GCTGGCTCTC
    CTCACAGAAC TCTCCCAGAA TCGCAGCAGT GAGAGCTACA GGCCATTCAG TGGCTCCCAG
    AGTGGCTCTG CTTTCAGCAG CATCTTCCAG AATGAGAATT TCCAGCTGCA GCTCATTCCC
    CCACCTGTGA CTGAGGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639848.1
    CDS177..4457
    Translation

    Target ORF information:

    RefSeq Version XM_005639791.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639791.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGATCAG
    AATCAAGCTT TGCGACTCTG TCTGGGTAGC ACTAGTGTTG GAGGTCAGTA TGGAGCCATC
    TCTGCCCTCA GTATCAACAA TGACTGCTCA AGACTTCTCT GTGGCTTTGC AAAAGGACAG
    ATCACGATGT GGGATTTGGC CAGTGGAAAA CTTCTAAGAT CAATAACAGA TGCTCATCCT
    CCAGGAACAG CTATACTGCA TATTAAGTTT ACAGATGATC CAACTCTTGC AATTTGCAAT
    GACAGCGGAG GCTCTGTTTT TGAATTGACA TTTAAGAGAG TGATGGGAGT GAGAACATGT
    GAATCTAGAT GTCTGTTCAG TGGTTCCAAG GGTGAAGTTT GCTGTATTGA GCCTCTCCAT
    TCAAAGCCTG AGTTAAAAGA TCATCCCATC ACACAGTTTT CCTTATTGGC CATGGCGTCC
    TTAACAAAGA TACTGGTCAT TGGATTGAAA CCATCTTTGA AAGTGTGGAT GACTTTTCCC
    TATGGACGGA TGGATCCTTC CAGTGTTCCA TTGCTGGCCT GGCACTTTGT GGCAGTGCAT
    AATTATGTGA ATCCCATGCT TGCCTTCTGC AGAGGAGATG TTGTTCATTT TCTGTTGGTT
    AAGAGAGATG AGTCTGGAGC AATACATGTT ACTAAGCAAA AGCACCTTCA TCTCTACTAT
    GATCTCATCA ACTTTACTTG GATAAACTCA CGCACAGTTG TGCTCTTGGA CAGTGTGGAG
    AAGTTGCATG TGATTGATCG GCAGACTCAA GAGGAATTGG AAACAGTGGA GATCTCAGAG
    GTTCAGTTGG TCTATAATAG CAGCCATTTC AAATCCCTAG CCACCGGAGG AAATGTTAGC
    CAGGCACTGG CTTTGGTTGG AGAGAAGGCT TGTTATCAAT CCATCAGTAG CTATGGTGGT
    CAGATCTTTT ATTTGGGGAC AAAATCTGTT TATGTGATGA TGCTGAGAAG CTGGAGAGAG
    AGAGTGGATC ATCTCCTGAA ACAAGATTGT CTTACAGAAG CCTTGGCTCT TGCATGGTCT
    TTCCATGAAG GAAAAGCAAA AGCAGTAGTG GGATTATCAG GGGATGCCAG TAAGCGAAAG
    GCTGTTGTTG CAGATCGGAT GGTAGAAATC CTGTTCCACT ATGCAGATCG AGCTCTGAAA
    AAGTGTCCAG ACCAGGGAAA AATCCAAGTG ATGGAGCAGC ATTTTCAGGA CATGGTACCT
    GTCATAGTTG ATTACTGCCT GCTGTTGCAA CGAAAGGATC TTTTATTTAG CCAGATGTAT
    GATAAACTAA GTGAGAACTC AGTGGCCAAA GGAGTGTTTT TGGAGTGCCT TGAGCCATAT
    ATTTTAAGTG ATAAATTGGT GGGGATGACC CCCCAGGTAA TGAAAGACTT GATTGTTCAT
    TTCCAAGATA AAAAGTTGAT GGAAAATGTG GAAGCCCTCA TTGTACATAT GGATATCACC
    AGCCTCGATA TTCAGCAGGT AGTTCTCATG TGTTGGGAAA ATCGTCTGTA TGATGCCATG
    ATCTATGTCT ACAACAGAGG CATGAATGAA TTTATTAGTC CAATGGAGAA ACTTTTCAGA
    GTCATTGCTC CTCCTCTGAA TGCAGGAAGG ACACTAACAG ATGAGCAAGT TGTTATGGGC
    AACAAGCTTC TCGTATATAT TAGCTGTTGT CTTGCAGGCC GAGCCTATCC TCTTGGTGAC
    ATCCCTGAAG ATCTTGTTCC CTTGGTTAAA AACCAGGTTT TTGAATTTCT GATTCGCCTG
    CATTCAGCAG AAGCTTCCCC TGAGGAAGAA ATCTACCCCT ATGTTCGAAC TCTGCTACAC
    TTTGACACAA GAGAATTTCT AAATGTTTTG GCACTGACTT TTGAAGATTT TAAAAATGAC
    AAGCAAGCTG TAGAATATCA ACAACGAATT GTGGATATTT TGTTGAAAGT TATGGTGGAG
    AATTCTGATT TTACTCCCTC ACAAGTAGGG TGTCTCTTTA CATTCCTTGC CCGGCAGCTT
    GCAAAGCCTG ACAATACCTT ATTTGTAAAC AGAACCCTTT TTGATCAGGT CCTTGAATTC
    CTCTGCAGTC CTGATGATGA CTCCCGACAC TCTGAAAGAC AGCAGGTCCT TTTAGAGTTG
    CTGCAAGCTG GAGGCATAGT TCAGTTTGAA GAGAGTCGGC TTATCCGGAT GGCAGAAAAA
    GCAGAATTCT ATCAAATTTG TGAGTTTATG TATGAACGAG AACACCAATA TGACAAAATA
    ATCGATTGCT ACTTACGTGA CCCACTAAGG GAGGAAGAAG TCTTTAACTA TATTCACAAT
    ATATTATCCA TTCCTGGACA CAGTGCAGAG GAGAAGCAAT CTGTATGGCA GAAAGCAATG
    GATCATATCG AGGAACTTGT ATCCCTGAGG CCCTGTAAAG CTGCAGAGCT GGTTGCTACT
    CACTTTTCTG AACAGATTGA AACAGTCATT AAAAAACTCC AGAACCAAGT TTTGCTTTTC
    AAATTTTTGA GGAGTCTTCT TGACCCAAGG GAAGGTATTC ATGTAAATCA AGAATTGCTG
    CAGATGTCTC CCTGTGTTAC GGAGCAGTTC ATTGAGCTGC TATGTCAGTT CAGCCCAAAC
    CAGGTTATAG AGACACTGCA GGTCCTTGAG TGCTACCGCC TGGAAGAAAC CATCCAGATT
    ACCCAAAAGT ATCAACTTCA TGAAGTCACT GCCTATCTAT TAGAAAAGAA AGGAGATATT
    CATGGTGCCT TCTTAATAAT GCTAAAGAGG CTACAGAGCA AACTTCAAGA AATAACACAT
    CAAGATGAAA AATCCAAAGA AGATCTCTCA CTGAAGGATG TTGAAGATAC CATGGTAGAG
    ACAATTGCCC TTTGCCAGAG AAATTCACAT AATTTGAACC AGCAGCAACG AGAGGCACTC
    TGGTTTCCAT TATTGGAGGC AATGATGGCT CCTCAGAAGC TGTCTGGTTC AGCTGCGCCT
    CATCTACACC CTGAAGCTCT GAAATCTTTG ACCATGCAAG TTCTAAACAG CATGGCAGCA
    TTTATTGCTC TTCCATCAAT CTTGCAAAGA ATCTTACAGG ATCCAGTTTA TGGAAAAGGA
    AAACTTGGAG AAATCCAGGG ACTTATTCTG GGAATGTTGG ATACCTTTAA TTATGAACAA
    ACCCTGCTGG AAACAACTAC TAGCCTTCTA AACCAAGATC TCCATTGGTC ATTGTGTAAC
    CTGAGAGCTT CAGTCACCAG AGGACTGAAT CCCAAGCAGG ATTACTGCTC TATATGTTTG
    CAGCAATACA AGAGACGCCA AGAAATGGCT GATGAAATCA TTGTCTTTAG CTGTGGCCAT
    TTGTATCACT CATTCTGTCT GCAAAACAAA GAGTGCACCA TAGAAACTGA GGGGCAAACA
    AGATGGACAT GCTACAAATG CAGCTCAAGT AACAAAGGAG GGAAACTAAG TGAAAATTCA
    TCTGAAATTA AAAAGGGAAA GACAACCTCA TCACAGGTAA AAATGTCTCC TTCTTGTCAT
    CCATCCAAAG GAGATTCTAC TGTTAAGAAG GCAGCCTCAG AACCCGTTCT GGATCCACAG
    CAAATCCAAG CATTTGATCA GCTCTGCCGT CTCTACCGAG GAAGTTCCCG GCTGGCTCTC
    CTCACAGAAC TCTCCCAGAA TCGCAGCAGT GAGAGCTACA GGCCATTCAG TGGCTCCCAG
    AGTGGCTCTG CTTTCAGCAG CATCTTCCAG AATGAGAATT TCCAGCTGCA GCTCATTCCC
    CCACCTGTGA CTGAGGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30483
    Clone ID Related Accession (Same CDS sequence) XM_022414311.1 , XM_005639792.2
    Accession Version XM_022414311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGACAG CTGTGCTCAA AGAAAACTGG
    CCCAAGATAT TAGCGTTCCA TACTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270019.1
    CDS1..4110
    Translation

    Target ORF information:

    RefSeq Version XM_022414311.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGACAG CTGTGCTCAA AGAAAACTGG
    CCCAAGATAT TAGCGTTCCA TACTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30483
    Clone ID Related Accession (Same CDS sequence) XM_022414311.1 , XM_005639792.2
    Accession Version XM_005639792.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGACAG CTGTGCTCAA AGAAAACTGG
    CCCAAGATAT TAGCGTTCCA TACTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639849.1
    CDS177..4286
    Translation

    Target ORF information:

    RefSeq Version XM_005639792.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639792.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGACAG CTGTGCTCAA AGAAAACTGG
    CCCAAGATAT TAGCGTTCCA TACTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30484
    Clone ID Related Accession (Same CDS sequence) XM_022414313.1 , XM_005639793.2
    Accession Version XM_022414313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGATTT TACTTATTTA TTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270021.1
    CDS1..4077
    Translation

    Target ORF information:

    RefSeq Version XM_022414313.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris VPS8, CORVET complex subunit (VPS8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGATTT TACTTATTTA TTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30484
    Clone ID Related Accession (Same CDS sequence) XM_022414313.1 , XM_005639793.2
    Accession Version XM_005639793.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product vacuolar protein sorting-associated protein 8 homolog isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726120.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGATTT TACTTATTTA TTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639850.1
    CDS177..4253
    Translation

    Target ORF information:

    RefSeq Version XM_005639793.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639793.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAAAATG AACCAGACCA TGAAAATGTG GAACAGAGCC TTTGTGCCAA GACTACTGAA 
    GAAGAGCTGA ATAAGTCTTT CAATCTTGAA GCTTCCCTTT CAAAATTCTC TTACATAGAT
    TTGGACAAGG AACTGGAGTT CAAAAATGAT CTGATTGATG ACAAGGAGTT TGATATTCCT
    CAAGTTGATA CTCCACCAAC ACTGGAAAGC ATACTCAATG AGACTGATGA TGAAGATGAG
    TCTTTTGTTC TTGAGGATCC TACGTTGTTA AACATTGATA CTATTGATTC TCACTCCTAT
    GATACTTCCT CTGTGGCAAG TTCAGATAGT GGTGACAGGA CCAACCTAAA AAGGAAGAAG
    AAATTACCTG ATTCTTTTTC ACTCCATGGG TCAGTTATGC GACATTCACT TTTGAAGGGA
    ATTTCTGCCC AGATAGTGTC TGCAGCTGAC AAAGTGGATG CTGGCTTGCC TACAGCAATT
    GCAGTGTCCA GTCTGATAGC TGTGGGTACA TCTCATGGAT TGGCTTTAAT ATTTGGAAAA
    GATCAGAATC AAGCTTTGCG ACTCTGTCTG GGTAGCACTA GTGTTGGAGG TCAGTATGGA
    GCCATCTCTG CCCTCAGTAT CAACAATGAC TGCTCAAGAC TTCTCTGTGG CTTTGCAAAA
    GGACAGATCA CGATGTGGGA TTTGGCCAGT GGAAAACTTC TAAGATCAAT AACAGATGCT
    CATCCTCCAG GAACAGCTAT ACTGCATATT AAGTTTACAG ATGATCCAAC TCTTGCAATT
    TGCAATGACA GCGGAGGCTC TGTTTTTGAA TTGACATTTA AGAGAGTGAT GGGAGTGAGA
    ACATGTGAAT CTAGATGTCT GTTCAGTGGT TCCAAGGGTG AAGTTTGCTG TATTGAGCCT
    CTCCATTCAA AGCCTGAGTT AAAAGATCAT CCCATCACAC AGTTTTCCTT ATTGGCCATG
    GCGTCCTTAA CAAAGATACT GGTCATTGGA TTGAAACCAT CTTTGAAAGT GTGGATGACT
    TTTCCCTATG GACGGATGGA TCCTTCCAGT GTTCCATTGC TGGCCTGGCA CTTTGTGGCA
    GTGCATAATT ATGTGAATCC CATGCTTGCC TTCTGCAGAG GAGATGTTGT TCATTTTCTG
    TTGGTTAAGA GAGATGAGTC TGGAGCAATA CATGTTACTA AGCAAAAGCA CCTTCATCTC
    TACTATGATC TCATCAACTT TACTTGGATA AACTCACGCA CAGTTGTGCT CTTGGACAGT
    GTGGAGAAGT TGCATGTGAT TGATCGGCAG ACTCAAGAGG AATTGGAAAC AGTGGAGATC
    TCAGAGGTTC AGTTGGTCTA TAATAGCAGC CATTTCAAAT CCCTAGCCAC CGGAGGAAAT
    GTTAGCCAGG CACTGGCTTT GGTTGGAGAG AAGGCTTGTT ATCAATCCAT CAGTAGCTAT
    GGTGGTCAGA TCTTTTATTT GGGGACAAAA TCTGTTTATG TGATGATGCT GAGAAGCTGG
    AGAGAGAGAG TGGATCATCT CCTGAAACAA GATTGTCTTA CAGAAGCCTT GGCTCTTGCA
    TGGTCTTTCC ATGAAGGAAA AGCAAAAGCA GTAGTGGGAT TATCAGGGGA TGCCAGTAAG
    CGAAAGGCTG TTGTTGCAGA TCGGATGGTA GAAATCCTGT TCCACTATGC AGATCGAGCT
    CTGAAAAAGT GTCCAGACCA GGGAAAAATC CAAGTGATGG AGCAGCATTT TCAGGACATG
    GTACCTGTCA TAGTTGATTA CTGCCTGCTG TTGCAACGAA AGGATCTTTT ATTTAGCCAG
    ATGTATGATA AACTAAGTGA GAACTCAGTG GCCAAAGGAG TGTTTTTGGA GTGCCTTGAG
    CCATATATTT TAAGTGATAA ATTGGTGGGG ATGACCCCCC AGGTAATGAA AGACTTGATT
    GTTCATTTCC AAGATAAAAA GTTGATGGAA AATGTGGAAG CCCTCATTGT ACATATGGAT
    ATCACCAGCC TCGATATTCA GCAGGTAGTT CTCATGTGTT GGGAAAATCG TCTGTATGAT
    GCCATGATCT ATGTCTACAA CAGAGGCATG AATGAATTTA TTAGTCCAAT GGAGAAACTT
    TTCAGAGTCA TTGCTCCTCC TCTGAATGCA GGAAGGACAC TAACAGATGA GCAAGTTGTT
    ATGGGCAACA AGCTTCTCGT ATATATTAGC TGTTGTCTTG CAGGCCGAGC CTATCCTCTT
    GGTGACATCC CTGAAGATCT TGTTCCCTTG GTTAAAAACC AGGTTTTTGA ATTTCTGATT
    CGCCTGCATT CAGCAGAAGC TTCCCCTGAG GAAGAAATCT ACCCCTATGT TCGAACTCTG
    CTACACTTTG ACACAAGAGA ATTTCTAAAT GTTTTGGCAC TGACTTTTGA AGATTTTAAA
    AATGACAAGC AAGCTGTAGA ATATCAACAA CGAATTGTGG ATATTTTGTT GAAAGTTATG
    GTGGAGAATT CTGATTTTAC TCCCTCACAA GTAGGGTGTC TCTTTACATT CCTTGCCCGG
    CAGCTTGCAA AGCCTGACAA TACCTTATTT GTAAACAGAA CCCTTTTTGA TCAGGTCCTT
    GAATTCCTCT GCAGTCCTGA TGATGACTCC CGACACTCTG AAAGACAGCA GGTCCTTTTA
    GAGTTGCTGC AAGCTGGAGG CATAGTTCAG TTTGAAGAGA GTCGGCTTAT CCGGATGGCA
    GAAAAAGCAG AATTCTATCA AATTTGTGAG TTTATGTATG AACGAGAACA CCAATATGAC
    AAAATAATCG ATTGCTACTT ACGTGACCCA CTAAGGGAGG AAGAAGTCTT TAACTATATT
    CACAATATAT TATCCATTCC TGGACACAGT GCAGAGGAGA AGCAATCTGT ATGGCAGAAA
    GCAATGGATC ATATCGAGGA ACTTGTATCC CTGAGGCCCT GTAAAGCTGC AGAGCTGGTT
    GCTACTCACT TTTCTGAACA GATTGAAACA GTCATTAAAA AACTCCAGAA CCAAGTTTTG
    CTTTTCAAAT TTTTGAGGAG TCTTCTTGAC CCAAGGGAAG GTATTCATGT AAATCAAGAA
    TTGCTGCAGA TGTCTCCCTG TGTTACGGAG CAGTTCATTG AGCTGCTATG TCAGTTCAGC
    CCAAACCAGG TTATAGAGAC ACTGCAGGTC CTTGAGTGCT ACCGCCTGGA AGAAACCATC
    CAGATTACCC AAAAGTATCA ACTTCATGAA GTCACTGCCT ATCTATTAGA AAAGAAAGGA
    GATATTCATG GTGCCTTCTT AATAATGCTA AAGAGGCTAC AGAGCAAACT TCAAGAAATA
    ACACATCAAG ATGAAAAATC CAAAGAAGAT CTCTCACTGA AGGATGTTGA AGATACCATG
    GTAGAGACAA TTGCCCTTTG CCAGAGAAAT TCACATAATT TGAACCAGCA GCAACGAGAG
    GCACTCTGGT TTCCATTATT GGAGGCAATG ATGGCTCCTC AGAAGCTGTC TGGTTCAGCT
    GCGCCTCATC TACACCCTGA AGCTCTGAAA TCTTTGACCA TGCAAGTTCT AAACAGCATG
    GCAGCATTTA TTGCTCTTCC ATCAATCTTG CAAAGAATCT TACAGGATCC AGTTTATGGA
    AAAGGAAAAC TTGGAGAAAT CCAGGGACTT ATTCTGGGAA TGTTGGATAC CTTTAATTAT
    GAACAAACCC TGCTGGAAAC AACTACTAGC CTTCTAAACC AAGATCTCCA TTGGTCATTG
    TGTAACCTGA GAGCTTCAGT CACCAGAGGA CTGAATCCCA AGCAGGATTA CTGCTCTATA
    TGTTTGCAGC AATACAAGAG ACGCCAAGAA ATGGCTGATG AAATCATTGT CTTTAGCTGT
    GGCCATTTGT ATCACTCATT CTGTCTGCAA AACAAAGAGT GCACCATAGA AACTGAGGGG
    CAAACAAGAT GGACATGCTA CAAATGCAGC TCAAGTAACA AAGGAGGGAA ACTAAGTGAA
    AATTCATCTG AAATTAAAAA GGGAAAGACA ACCTCATCAC AGGTAAAAAT GTCTCCTTCT
    TGTCATCCAT CCAAAGGAGA TTCTACTGTT AAGAAGATTT TACTTATTTA TTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.