ORF » Species Summary » Schizosaccharomyces pombe » SPBC32F12.07c cDNA ORF clone

SPBC32F12.07c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPBC32F12.07c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC32F12.07c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100676 NM_001022069.2
Latest version!		 Schizosaccharomyces pombe putative MARCH family ubiquitin-protein ligase E3 (SPBC32F12.07c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPBC32F12.07c ( NM_001022069.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596150.1 Schizosaccharomyces pombe putative MARCH family ubiquitin-protein ligase E3 (SPBC32F12.07c), mRNA.
    Price & Availability↑
    CloneID OSc100676
    Clone ID Related Accession (Same CDS sequence) NM_001022069.2
    Accession Version NM_001022069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative MARCH family ubiquitin-protein ligase E3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022069.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACGGACA CCGCGAAGTA TGAGAAAAGC AGTGCTCGCT GCTGGATATG CTATGAGGAA 
    TACGACAAAA AACTATGCTC CCTTTCAAAT GATTCTTGGA GACGTCCGTG TCGGTGTTCA 
    CTCATTGCTC ATGAAAGTTG TTTGATTTCT TACATTACCA GATCTGGCAG TACTCGTTGT 
    CCTCAATGTC TTACCGCATA TAGAATTGCT AAACCGCCAA AGGAAAAGTC TTGGGCTGTA 
    AACGTTCTTG GAATCGGTCA TTCCCTTGAA GCTGGTCTAG CTCAAGTAAC GTTTGGTGTA 
    GGTTCTTGTT TAGGTATTAC CAAGTTTATT TATTCAATAT TCAAACAGAC TGGAATATGG 
    ATATGTAAAC AGGTGGCAGA TGAAAGCAGC CTGATAGAAA TGTTAAAAAA GCCAGTTTTC 
    TCCTCTGTCG TATTGCCTTT ATTACCATGC ATGTTGGTTC GGTTCTACGA AGCTCCGCCT 
    TATGATATAG CATTTAGTCT CTACACGCAT TTTTCAATTT ATAGTTGCGC GGAAAAGATT 
    TCGAATACCT CACTTCTACT GTGCACTCTG CCTTGGGTAA GATCTTTATA TAAGGAACTA 
    ATGACCCGTA TATTTGACGG TATTGTAATT GGAGCGGACG GGGAATTTGA AGATTCTGAA 
    ACTGATTGGT TTCGCCAGTT TGAAGCACAA GTAGAGCATC GTAACCAAGT CGAGGATGTA 
    AACGAAAGAG AAGATACAGA ATCTGAATTT TGGATTCTTT TGTCTGTGGC ACACGTTTTC 
    CTTGATGCTT TTACTACGAA AATTCTCCGT ATTGTTCGAC CGATTCTACT ATTCCCATTA 
    GCAGGGAAAT TTTTGGGAAG GTTTATTCCA GGGAATTTCA CAAAGTTAGA GAAGAGCATT 
    ATTGGTGCCT TTGCTGCATT AGTTTTTAAG GACATTTTTG TCTATGGTTT CATTGCTTGG 
    AGAAAGAGAA AACCCTGGAG CATTCGAATT CTCGACAATC CTCGTAGAGT TTCTGACTCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596150.1
    CDS432..1454
    Translation

    Target ORF information:

    RefSeq Version NM_001022069.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative MARCH family ubiquitin-protein ligase E3 (SPBC32F12.07c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACGGACA CCGCGAAGTA TGAGAAAAGC AGTGCTCGCT GCTGGATATG CTATGAGGAA 
    TACGACAAAA AACTATGCTC CCTTTCAAAT GATTCTTGGA GACGTCCGTG TCGGTGTTCA 
    CTCATTGCTC ATGAAAGTTG TTTGATTTCT TACATTACCA GATCTGGCAG TACTCGTTGT 
    CCTCAATGTC TTACCGCATA TAGAATTGCT AAACCGCCAA AGGAAAAGTC TTGGGCTGTA 
    AACGTTCTTG GAATCGGTCA TTCCCTTGAA GCTGGTCTAG CTCAAGTAAC GTTTGGTGTA 
    GGTTCTTGTT TAGGTATTAC CAAGTTTATT TATTCAATAT TCAAACAGAC TGGAATATGG 
    ATATGTAAAC AGGTGGCAGA TGAAAGCAGC CTGATAGAAA TGTTAAAAAA GCCAGTTTTC 
    TCCTCTGTCG TATTGCCTTT ATTACCATGC ATGTTGGTTC GGTTCTACGA AGCTCCGCCT 
    TATGATATAG CATTTAGTCT CTACACGCAT TTTTCAATTT ATAGTTGCGC GGAAAAGATT 
    TCGAATACCT CACTTCTACT GTGCACTCTG CCTTGGGTAA GATCTTTATA TAAGGAACTA 
    ATGACCCGTA TATTTGACGG TATTGTAATT GGAGCGGACG GGGAATTTGA AGATTCTGAA 
    ACTGATTGGT TTCGCCAGTT TGAAGCACAA GTAGAGCATC GTAACCAAGT CGAGGATGTA 
    AACGAAAGAG AAGATACAGA ATCTGAATTT TGGATTCTTT TGTCTGTGGC ACACGTTTTC 
    CTTGATGCTT TTACTACGAA AATTCTCCGT ATTGTTCGAC CGATTCTACT ATTCCCATTA 
    GCAGGGAAAT TTTTGGGAAG GTTTATTCCA GGGAATTTCA CAAAGTTAGA GAAGAGCATT 
    ATTGGTGCCT TTGCTGCATT AGTTTTTAAG GACATTTTTG TCTATGGTTT CATTGCTTGG 
    AGAAAGAGAA AACCCTGGAG CATTCGAATT CTCGACAATC CTCGTAGAGT TTCTGACTCA 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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