spi1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following spi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101258 NM_001023848.2
Latest version!
Schizosaccharomyces pombe Ran GTPase Spi1 (spi1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc101258
    Clone ID Related Accession (Same CDS sequence) NM_001023848.2
    Accession Version NM_001023848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product Ran GTPase Spi1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001023848.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCTCAAC CACAAAACGT TCCTACCTTT AAGCTTGTAC TTGTCGGCGA TGGTGGTACC 
    GGTAAGACCA CGTTCGTCAA GCGTCATTTG ACTGGTGAAT TTGAAAAGAA GTATATTGCA
    ACTTTGGGTG TTGAGGTCCA TCCTTTGCAT TTTCATACTA ATTTTGGTGA AATTTGCTTT
    AATGTTTGGG ATACCGCTGG TCAAGAAAAG TTAGGTGGCC TTCGTGATGG TTACTATATT
    CAAGGTCAAT GTGGTATTAT CATGTTTGAT GTTACTTCCC GTATTACCTA TAAGAATGTT
    CCCCATTGGT GGCGTGATCT TGTCCGTGTT TGTGAAAACA TTCCCATTGT TCTTTGCGGT
    AACAAAGTTG ATGTTAAAGA GCGTAAAGTC AAGGCTAAGG CTATCACTTT CCATCGCAAG
    AAAAACCTTC AATACTACGA CATCTCCGCC AAGTCCAATT ATAACTTCGA AAAACCTTTC
    TTATGGCTTG CCCGTAAGCT GGTTGGCAAC CCCAACCTGG AGTTTGTTGC TTCGCCTGCC
    CTTGCTCCTC CTGAGGTTCA AGTCGATCAG CAATTGTTAG CTCAATACCA ACAGGAAATG
    AACGAGGCTG CTGCTATGCC TTTACCTGAT GAGGATGACG CTGATTTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596827.1
    CDS77..727
    Translation

    Target ORF information:

    RefSeq Version NM_001023848.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe Ran GTPase Spi1 (spi1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCTCAAC CACAAAACGT TCCTACCTTT AAGCTTGTAC TTGTCGGCGA TGGTGGTACC 
    GGTAAGACCA CGTTCGTCAA GCGTCATTTG ACTGGTGAAT TTGAAAAGAA GTATATTGCA
    ACTTTGGGTG TTGAGGTCCA TCCTTTGCAT TTTCATACTA ATTTTGGTGA AATTTGCTTT
    AATGTTTGGG ATACCGCTGG TCAAGAAAAG TTAGGTGGCC TTCGTGATGG TTACTATATT
    CAAGGTCAAT GTGGTATTAT CATGTTTGAT GTTACTTCCC GTATTACCTA TAAGAATGTT
    CCCCATTGGT GGCGTGATCT TGTCCGTGTT TGTGAAAACA TTCCCATTGT TCTTTGCGGT
    AACAAAGTTG ATGTTAAAGA GCGTAAAGTC AAGGCTAAGG CTATCACTTT CCATCGCAAG
    AAAAACCTTC AATACTACGA CATCTCCGCC AAGTCCAATT ATAACTTCGA AAAACCTTTC
    TTATGGCTTG CCCGTAAGCT GGTTGGCAAC CCCAACCTGG AGTTTGTTGC TTCGCCTGCC
    CTTGCTCCTC CTGAGGTTCA AGTCGATCAG CAATTGTTAG CTCAATACCA ACAGGAAATG
    AACGAGGCTG CTGCTATGCC TTTACCTGAT GAGGATGACG CTGATTTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L