Ran cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ran
Entrez Gene ID	84509
Full Name	RAN, member RAS oncogene family
General protein information	Preferred Names RAN, member RAS oncogene family Names GTP-binding nuclear protein Ran GTPase Ran ras-like protein TC4 ras-related nuclear protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q13
Summary	plays a role in nucleocytoplasmic protein transport; regulates microtubule assembly; may play a role in microtubule nucleation during spermiogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249285.3	XP_006249347.1	GTP-binding nuclear protein Ran isoform X1
NM_053439.1	NP_445891.1	GTP-binding nuclear protein Ran

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAN	XP_003952405.1
Homo sapiens (human)	RAN	NP_006316.1
	RAN	XP_001104422.1
Canis lupus familiaris (dog)	RAN	NP_001181913.1
Mus musculus (house mouse)	Ran	NP_033417.1
Gallus gallus (chicken)	RAN	NP_990589.1
Drosophila melanogaster (fruit fly)	ran	NP_727499.1
Saccharomyces cerevisiae (baker's yeast)	GSP1	NP_013396.1
Kluyveromyces lactis	KLLA0C05126g	XP_452429.1
Arabidopsis thaliana (thale cress)	RAN-1	NP_197501.1
Bos taurus (cattle)	RAN	NP_001029877.1
Danio rerio (zebrafish)	ran	NP_571384.1
Caenorhabditis elegans (roundworm)	ran-1	NP_499369.1
Eremothecium gossypii	AGOS_AGR294C	NP_986960.1
Neurospora crassa	NCU09269	XP_964704.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007699	XP_564524.3
Saccharomyces cerevisiae (baker's yeast)	GSP2	NP_014828.1
Schizosaccharomyces pombe (fission yeast)	spi1	NP_596827.1
Rattus norvegicus (Norway rat)	Ran	NP_445891.1
Kluyveromyces lactis	KLLA0A04499g	XP_451197.1
Magnaporthe oryzae (rice blast fungus)	MGG_09952	XP_003710062.1
Arabidopsis thaliana (thale cress)	RAN3	NP_200330.1
Arabidopsis thaliana (thale cress)	RAN2	NP_197502.1
Oryza sativa (rice)	Os01g0611100	NP_001043550.1
Xenopus tropicalis (tropical clawed frog)	ran	NP_001004829.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation.
Bao X, Liu H, Liu X, Ruan K, Zhang Y, Zhang Z, Hu Q, Liu Y, Akram S, Zhang J, Gong Q, Wang W, Yuan X, Li J, Zhao L, Dou Z, Tian R, Yao X, Wu J, Shi Y
Journal of molecular cell biology(2017 Oct)

Samp1, a RanGTP binding transmembrane protein in the inner nuclear membrane.
Vijayaraghavan B, Jafferali MH, Figueroa RA, Hallberg E
Nucleus (Austin, Tex.)7(4)415-23(2016 07)

Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
Rudack T, Jenrich S, Brucker S, Vetter IR, Gerwert K, K?tting C
The Journal of biological chemistry290(40)24079-90(2015 Oct)

The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.
Zhang K, Donnelly CJ, Haeusler AR, Grima JC, Machamer JB, Steinwald P, Daley EL, Miller SJ, Cunningham KM, Vidensky S, Gupta S, Thomas MA, Hong I, Chiu SL, Huganir RL, Ostrow LW, Matunis MJ, Wang J, Sattler R, Lloyd TE, Rothstein JD
Nature525(7567)56-61(2015 Sep)

The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes.
Shibano T, Mamada H, Hakuno F, Takahashi S, Taira M
PloS one10(5)e0127271(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we observed that microtubule network stabilization downregulated Ran-mediated pVHL nuclear accumulation, which in turn led to the upregulation of HIF-1alpha protein
Title: Role of Ran-regulated nuclear-cytoplasmic trafficking of pVHL in the regulation of microtubular stability-mediated HIF-1? in hypoxic cardiomyocytes.

RanGTP is indispensible in governing the genomic integrity and the fidelity of cell cycle progression.
Title: In vivo FRET imaging revealed a regulatory role of RanGTP in kinetochore-microtubule attachments via Aurora B kinase.

Data show that nuclear import of CAR is regulated by importin/Ran-GTP systems.
Title: The nuclear import of the constitutive androstane receptor by importin/Ran-GTP systems.

The RanGTPase system regulates the formation of retrograde importin signaling complexes in the axons of injured peripheral neurons.
Title: Localized regulation of axonal RanGTPase controls retrograde injury signaling in peripheral nerve.

Ran-GTPase may be involved in both nucleocytoplasmic transport and microtubule assembly, two critical events during the development of functional sperm.
Title: Ran, a GTP-binding protein involved in nucleocytoplasmic transport and microtubule nucleation, relocates from the manchette to the centrosome region during rat spermiogenesis.

The following Ran gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ran cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa34858	XM_006249285.3 Latest version!	Rattus norvegicus RAN, member RAS oncogene family (Ran), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa09584	NM_053439.1 Latest version!	Rattus norvegicus RAN, member RAS oncogene family (Ran), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ran ( XM_006249285.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249347.1 Rattus norvegicus RAN, member RAS oncogene family (Ran), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34858
Clone ID Related Accession (Same CDS sequence)	XM_006249285.3
Accession Version	XM_006249285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding nuclear protein Ran isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCC AGGGAGAGCC GCAGGTCCAG TTCAAGCTCG TCCTGGTGGG CGACGGCGGC 
ACCGGGAAGA CGACGTTCGT GAAGCGCCAC TTGACGGGCG AGTTTGAGAA GAAGTATGTA 
GCCACCCTGG GCGTGGAGGT GCACCCGCTC GTCTTCCATA CCAACAGAGG ACCCATCAAG 
TTCAACGTGT GGGACACAGC CGGCCAGGAG AAGTTCGGGG GCCTGCGCGA TGGCTACTAC 
ATCCAAGCCC AGTGTGCCAT TATAATGTTT GACGTAACAT CAAGAGTTAC TTACAAGAAC 
GTACCTAACT GGCATAGAGA TCTGGTACGC GTGTGTGAAA ACATCCCCAT TGTATTGTGT 
GGCAACAAAG TGGATATTAA AGACAGGAAA GTGAAGGCAA AATCTATTGT CTTCCACCGA 
AAGAAGAATC TTCAGTACTA TGACATTTCT GCCAAAAGTA ACTACAACTT TGAAAAGCCT 
TTCCTCTGGC TTGCCAGAAA GCTCATTGGA GATCCTAACT TGGAGTTCGT TGCCATGCCT 
GCTCTTGCCC CACCTGAGGT GGTCATGGAC CCAGCTTTGG CAGCACAGTA CGAGCATGAT 
TTAGAGGTTG CTCAGACGAC TGCGCTCCCG GATGAGGATG ACGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249347.1
CDS	407..1057
Translation	MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

Target ORF information:

RefSeq Version	XM_006249285.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAN, member RAS oncogene family (Ran), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249285.3

ORF Insert Sequence:

ATGGCCGCCC AGGGAGAGCC GCAGGTCCAG TTCAAGCTCG TCCTGGTGGG CGACGGCGGC 
ACCGGGAAGA CGACGTTCGT GAAGCGCCAC TTGACGGGCG AGTTTGAGAA GAAGTATGTA 
GCCACCCTGG GCGTGGAGGT GCACCCGCTC GTCTTCCATA CCAACAGAGG ACCCATCAAG 
TTCAACGTGT GGGACACAGC CGGCCAGGAG AAGTTCGGGG GCCTGCGCGA TGGCTACTAC 
ATCCAAGCCC AGTGTGCCAT TATAATGTTT GACGTAACAT CAAGAGTTAC TTACAAGAAC 
GTACCTAACT GGCATAGAGA TCTGGTACGC GTGTGTGAAA ACATCCCCAT TGTATTGTGT 
GGCAACAAAG TGGATATTAA AGACAGGAAA GTGAAGGCAA AATCTATTGT CTTCCACCGA 
AAGAAGAATC TTCAGTACTA TGACATTTCT GCCAAAAGTA ACTACAACTT TGAAAAGCCT 
TTCCTCTGGC TTGCCAGAAA GCTCATTGGA GATCCTAACT TGGAGTTCGT TGCCATGCCT 
GCTCTTGCCC CACCTGAGGT GGTCATGGAC CCAGCTTTGG CAGCACAGTA CGAGCATGAT 
TTAGAGGTTG CTCAGACGAC TGCGCTCCCG GATGAGGATG ACGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ran ( NM_053439.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445891.1 Rattus norvegicus RAN, member RAS oncogene family (Ran), mRNA.

Price & Availability↑

CloneID	ORa09584
Clone ID Related Accession (Same CDS sequence)	NM_053439.1
Accession Version	NM_053439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding nuclear protein Ran
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF306457.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF306457.1, FQ211210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906352 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCCC AGGGAGAGCC GCAGGTCCAG TTCAAGCTCG TCCTGGTGGG CGACGGCGGC 
ACCGGGAAGA CGACGTTCGT GAAGCGCCAC TTGACGGGCG AGTTTGAGAA GAAGTATGTA 
GCCACCCTGG GCGTGGAGGT GCACCCGCTC GTCTTCCATA CCAACAGAGG ACCCATCAAG 
TTCAACGTGT GGGACACAGC CGGCCAGGAG AAGTTCGGGG GCCTGCGCGA TGGCTACTAC 
ATCCAAGCCC AGTGTGCCAT TATAATGTTT GACGTAACAT CAAGAGTTAC TTACAAGAAC 
GTACCTAACT GGCATAGAGA TCTGGTACGC GTGTGTGAAA ACATCCCCAT TGTATTGTGT 
GGCAACAAAG TGGATATTAA AGACAGGAAA GTGAAGGCAA AATCTATTGT CTTTCACCGA 
AAGAAGAATC TTCAGTACTA TGACATTTCT GCCAAAAGTA ACTACAACTT TGAAAAGCCT 
TTCCTCTGGC TTGCCAGAAA GCTCATTGGA GATCCTAACT TGGAGTTCGT TGCCATGCCT 
GCTCTTGCCC CACCTGAGGT GGTCATGGAC CCAGCTTTGG CAGCACAGTA CGAGCATGAT 
TTAGAGGTTG CTCAGACGAC TGCGCTCCCG GATGAGGATG ACGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445891.1
CDS	73..723
Translation	MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

Target ORF information:

RefSeq Version	NM_053439.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAN, member RAS oncogene family (Ran), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053439.1

ORF Insert Sequence:

ATGGCCGCCC AGGGAGAGCC GCAGGTCCAG TTCAAGCTCG TCCTGGTGGG CGACGGCGGC 
ACCGGGAAGA CGACGTTCGT GAAGCGCCAC TTGACGGGCG AGTTTGAGAA GAAGTATGTA 
GCCACCCTGG GCGTGGAGGT GCACCCGCTC GTCTTCCATA CCAACAGAGG ACCCATCAAG 
TTCAACGTGT GGGACACAGC CGGCCAGGAG AAGTTCGGGG GCCTGCGCGA TGGCTACTAC 
ATCCAAGCCC AGTGTGCCAT TATAATGTTT GACGTAACAT CAAGAGTTAC TTACAAGAAC 
GTACCTAACT GGCATAGAGA TCTGGTACGC GTGTGTGAAA ACATCCCCAT TGTATTGTGT 
GGCAACAAAG TGGATATTAA AGACAGGAAA GTGAAGGCAA AATCTATTGT CTTTCACCGA 
AAGAAGAATC TTCAGTACTA TGACATTTCT GCCAAAAGTA ACTACAACTT TGAAAAGCCT 
TTCCTCTGGC TTGCCAGAAA GCTCATTGGA GATCCTAACT TGGAGTTCGT TGCCATGCCT 
GCTCTTGCCC CACCTGAGGT GGTCATGGAC CCAGCTTTGG CAGCACAGTA CGAGCATGAT 
TTAGAGGTTG CTCAGACGAC TGCGCTCCCG GATGAGGATG ACGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation.
Journal of molecular cell biology(2017 Oct)
Bao X,Liu H,Liu X,Ruan K,Zhang Y,Zhang Z,Hu Q,Liu Y,Akram S,Zhang J,Gong Q,Wang W,Yuan X,Li J,Zhao L,Dou Z,Tian R,Yao X,Wu J,Shi Y

Samp1, a RanGTP binding transmembrane protein in the inner nuclear membrane.
Nucleus (Austin, Tex.)7(4)415-23(2016 07)
Vijayaraghavan B,Jafferali MH,Figueroa RA,Hallberg E

Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
The Journal of biological chemistry290(40)24079-90(2015 Oct)
Rudack T,Jenrich S,Brucker S,Vetter IR,Gerwert K,K?tting C

The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.
Nature525(7567)56-61(2015 Sep)
Zhang K,Donnelly CJ,Haeusler AR,Grima JC,Machamer JB,Steinwald P,Daley EL,Miller SJ,Cunningham KM,Vidensky S,Gupta S,Thomas MA,Hong I,Chiu SL,Huganir RL,Ostrow LW,Matunis MJ,Wang J,Sattler R,Lloyd TE,Rothstein JD

The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes.
PloS one10(5)e0127271(2015)
Shibano T,Mamada H,Hakuno F,Takahashi S,Taira M

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Ran cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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