Ran cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ran
Entrez Gene ID	44072
Full Name	CG1404 gene product from transcript CG1404-RC
Synonyms	AAF30287,CG1404,Dmel\CG1404,Q9VZ23,RanGTP,dran,l(1)G0075,ran,ran10A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10A8-10A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_167272.3	NP_727499.1	Ran, isoform B
NM_001298182.1	NP_001285111.1	Ran, isoform C
NM_001298183.1	NP_001285112.1	Ran, isoform D
NM_143712.4	NP_651969.1	Ran, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAN	XP_003952405.1
Homo sapiens (human)	RAN	NP_006316.1
	RAN	XP_001104422.1
Canis lupus familiaris (dog)	RAN	NP_001181913.1
Mus musculus (house mouse)	Ran	NP_033417.1
Gallus gallus (chicken)	RAN	NP_990589.1
Drosophila melanogaster (fruit fly)	ran	NP_727499.1
Saccharomyces cerevisiae (baker's yeast)	GSP1	NP_013396.1
Kluyveromyces lactis	KLLA0C05126g	XP_452429.1
Arabidopsis thaliana (thale cress)	RAN-1	NP_197501.1
Bos taurus (cattle)	RAN	NP_001029877.1
Danio rerio (zebrafish)	ran	NP_571384.1
Caenorhabditis elegans (roundworm)	ran-1	NP_499369.1
Eremothecium gossypii	AGOS_AGR294C	NP_986960.1
Neurospora crassa	NCU09269	XP_964704.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007699	XP_564524.3
Saccharomyces cerevisiae (baker's yeast)	GSP2	NP_014828.1
Schizosaccharomyces pombe (fission yeast)	spi1	NP_596827.1
Rattus norvegicus (Norway rat)	Ran	NP_445891.1
Kluyveromyces lactis	KLLA0A04499g	XP_451197.1
Magnaporthe oryzae (rice blast fungus)	MGG_09952	XP_003710062.1
Arabidopsis thaliana (thale cress)	RAN3	NP_200330.1
Arabidopsis thaliana (thale cress)	RAN2	NP_197502.1
Oryza sativa (rice)	Os01g0611100	NP_001043550.1
Xenopus tropicalis (tropical clawed frog)	ran	NP_001004829.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RanGTP is required for meiotic spindle organization and the initiation of embryonic development.
Title: RanGTP is required for meiotic spindle organization and the initiation of embryonic development in Drosophila.

the Canoe Ras-association (RA) domains directly bind RanGTP
Title: Canoe binds RanGTP to promote Pins(TPR)/Mud-mediated spindle orientation.

Knockdown of the small G protein Ran, which is essential for nuclear transport, leads to an arrest of EcR in the cytoplasm, but does not prevent efficient nuclear import of the most important heterodimerization partner of EcR, ultraspiracle (Usp).
Title: The importance of exportin and Ran for nucleocytoplasmic shuttling of the ecdysteroid receptor.

the possibility that Dntf-2r and ran-like might be involved in genomic conflicts during spermatogenesis
Title: Convergently recruited nuclear transport retrogenes are male biased in expression and evolving under positive selection in Drosophila.

results demonstrate that distinctly from its role in spindle assembly, RanGTP maintains spindle microtubules in anaphase through the local activation of ISWI and that this is essential for proper chromosome segregation
Title: ISWI is a RanGTP-dependent MAP required for chromosome segregation.

The following Ran gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ran cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02310	NM_167272.3 Latest version!	Drosophila melanogaster Ran, transcript variant B (Ran), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa02310	NM_001298182.1 Latest version!	Drosophila melanogaster Ran, transcript variant C (Ran), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa02310	NM_001298183.1 Latest version!	Drosophila melanogaster Ran, transcript variant D (Ran), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa02310	NM_143712.4 Latest version!	Drosophila melanogaster Ran, transcript variant A (Ran), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ran ( NM_167272.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727499.1 Drosophila melanogaster Ran, transcript variant B (Ran), mRNA.

Price & Availability↑

CloneID	OFa02310
Clone ID Related Accession (Same CDS sequence)	NM_167272.3 , NM_001298182.1 , NM_001298183.1 , NM_143712.4
Accession Version	NM_167272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ran, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167272.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727499.1
CDS	147..797
Translation	MAQEGQDIPTFKCVLVGDGGTGKTTFVKRHMTGEFEKKYVATLGVEVHPLIFHTNRGAIRFNVWDTAGQEKFGGLRDGYYIQGQCAVIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGDPNLEFVAMPALLPPEVKMDKDWQAQIERDLQEAQATALPDEDEEL

Target ORF information:

RefSeq Version	NM_167272.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ran, transcript variant B (Ran), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167272.3

ORF Insert Sequence:

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ran ( NM_001298182.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285111.1 Drosophila melanogaster Ran, transcript variant C (Ran), mRNA.

Price & Availability↑

CloneID	OFa02310
Clone ID Related Accession (Same CDS sequence)	NM_167272.3 , NM_001298182.1 , NM_001298183.1 , NM_143712.4
Accession Version	NM_001298182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ran, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285111.1
CDS	134..784
Translation	MAQEGQDIPTFKCVLVGDGGTGKTTFVKRHMTGEFEKKYVATLGVEVHPLIFHTNRGAIRFNVWDTAGQEKFGGLRDGYYIQGQCAVIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGDPNLEFVAMPALLPPEVKMDKDWQAQIERDLQEAQATALPDEDEEL

Target ORF information:

RefSeq Version	NM_001298182.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ran, transcript variant C (Ran), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298182.1

ORF Insert Sequence:

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ran ( NM_001298183.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285112.1 Drosophila melanogaster Ran, transcript variant D (Ran), mRNA.

Price & Availability↑

CloneID	OFa02310
Clone ID Related Accession (Same CDS sequence)	NM_167272.3 , NM_001298182.1 , NM_001298183.1 , NM_143712.4
Accession Version	NM_001298183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ran, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285112.1
CDS	283..933
Translation	MAQEGQDIPTFKCVLVGDGGTGKTTFVKRHMTGEFEKKYVATLGVEVHPLIFHTNRGAIRFNVWDTAGQEKFGGLRDGYYIQGQCAVIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGDPNLEFVAMPALLPPEVKMDKDWQAQIERDLQEAQATALPDEDEEL

Target ORF information:

RefSeq Version	NM_001298183.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ran, transcript variant D (Ran), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298183.1

ORF Insert Sequence:

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ran ( NM_143712.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651969.1 Drosophila melanogaster Ran, transcript variant A (Ran), mRNA.

Price & Availability↑

CloneID	OFa02310
Clone ID Related Accession (Same CDS sequence)	NM_167272.3 , NM_001298182.1 , NM_001298183.1 , NM_143712.4
Accession Version	NM_143712.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ran, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_143712.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651969.1
CDS	220..870
Translation	MAQEGQDIPTFKCVLVGDGGTGKTTFVKRHMTGEFEKKYVATLGVEVHPLIFHTNRGAIRFNVWDTAGQEKFGGLRDGYYIQGQCAVIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGDPNLEFVAMPALLPPEVKMDKDWQAQIERDLQEAQATALPDEDEEL

Target ORF information:

RefSeq Version	NM_143712.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ran, transcript variant A (Ran), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143712.4

ORF Insert Sequence:

ATGGCTCAGG AAGGTCAGGA TATACCCACA TTCAAGTGCG TGCTAGTCGG CGATGGCGGC 
ACCGGCAAGA CCACCTTTGT CAAGCGGCAC ATGACCGGTG AGTTCGAGAA GAAGTACGTG 
GCCACACTGG GCGTGGAGGT GCATCCATTG ATCTTCCATA CCAATCGTGG CGCCATCCGT 
TTCAATGTGT GGGATACCGC TGGCCAGGAG AAGTTCGGCG GGCTGCGCGA TGGCTACTAC 
ATTCAGGGCC AGTGTGCCGT CATCATGTTC GATGTTACCT CGCGTGTCAC GTACAAGAAC 
GTGCCCAACT GGCATCGCGA TCTAGTCCGC GTCTGCGAGA ACATACCAAT CGTCCTCTGT 
GGCAACAAAG TCGATATCAA GGATCGCAAG GTGAAAGCGA AGAGCATCGT CTTCCACCGA 
AAGAAGAACT TGCAGTATTA CGATATTTCT GCCAAATCGA ACTACAACTT CGAGAAACCA 
TTCCTTTGGC TGGCGCGCAA GCTGGTTGGT GATCCCAACC TGGAGTTTGT CGCCATGCCA 
GCCCTGCTGC CGCCCGAGGT TAAGATGGAT AAGGATTGGC AGGCGCAGAT CGAGCGTGAC 
TTGCAGGAGG CCCAGGCGAC CGCACTGCCC GACGAGGACG AGGAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ran cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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