BCS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BCS1
Entrez Gene ID	851981
Full Name	bifunctional AAA family ATPase chaperone/translocase BCS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180683.1	NP_010663.1	bifunctional AAA family ATPase chaperone/translocase BCS1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCS1L	NP_004319.1
Pan troglodytes (chimpanzee)	BCS1L	XP_516092.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004266	XP_313188.3
Schizosaccharomyces pombe (fission yeast)	SPAC644.07	NP_593875.1
Xenopus tropicalis (tropical clawed frog)	bcs1l	NP_001016865.1
Canis lupus familiaris (dog)	BCS1L	XP_536070.1
Gallus gallus (chicken)	BCS1L	NP_001006520.1
Danio rerio (zebrafish)	bcs1l	NP_957476.2
Drosophila melanogaster (fruit fly)	CG4908	NP_609358.1
	BCS1L	XP_001092421.1
Kluyveromyces lactis	KLLA0E02487g	XP_454059.1
Bos taurus (cattle)	BCS1L	NP_001015671.1
Mus musculus (house mouse)	Bcs1l	NP_080060.1
Rattus norvegicus (Norway rat)	Bcs1l	NP_001007667.1
Caenorhabditis elegans (roundworm)	bcs-1	NP_001022191.1
Saccharomyces cerevisiae (baker's yeast)	BCS1	NP_010663.1
Eremothecium gossypii	AGOS_ACR200C	NP_983602.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The evolutionarily-conserved short alpha helix of Bcs1 in the intermembrane space is an essential element for the chaperone function.
Title: A conserved ? helix of Bcs1, a mitochondrial AAA chaperone, is required for the Respiratory Complex III maturation.

The ATP dependency of the protein Bcs1 is not just a requirement for its chaperon activity but also a way to couple the rate of complex III biogenesis to the energy-transducing activity of mitochondria.
Title: The energetic state of mitochondria modulates complex III biogenesis through the ATP-dependent activity of Bcs1.

The oligomeric AAA-ATPase Bcs1 is involved in export of the folded Fe-S domain of Rip1 across the inner membrane and insertion of its transmembrane segment into an assembly intermediate of the cytochrome bc(1) complex.
Title: A pathway of protein translocation in mitochondria mediated by the AAA-ATPase Bcs1.

residues located at the junction between the Bcs1p-specific and the AAA domains are important for the activity and stability of the protein, and the residue F342 is important for interactions with other partners or substrate proteins.
Title: Functional analysis of yeast bcs1 mutants highlights the role of Bcs1p-specific amino acids in the AAA domain.

The following BCS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01453	NM_001180683.1 Latest version!	Saccharomyces cerevisiae S288C bifunctional AAA family ATPase chaperone/translocase BCS1 (BCS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCS1 ( NM_001180683.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010663.1 Saccharomyces cerevisiae S288C bifunctional AAA family ATPase chaperone/translocase BCS1 (BCS1), partial mRNA.

Price & Availability↑

CloneID	OSi01453
Clone ID Related Accession (Same CDS sequence)	NM_001180683.1
Accession Version	NM_001180683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	bifunctional AAA family ATPase chaperone/translocase BCS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGGATA AGCCGATTGA CATACAATAT GATAAACAGG CTACTCCAAA TCTGAGTGGT 
GTAATTACTC CGCCAACAAA TGAAACTGGT AATGACTCAG TCCGGGAAAA ACTTTCAAAA 
CTAGTTGGCG ATGCTATGTC AAACAATCCC TATTTTGCCG CCGGCGGTGG TCTTATGATC 
CTTGGTACAG GTTTAGCTGT AGCTAGATCC GGTATAATAA AAGCCAGCAG GGTCCTATAC 
CGACAAATGA TTGTCGACTT AGAGATTCAG TCGAAGGACA AATCATACGC CTGGTTTCTA 
ACATGGATGG CCAAACATCC TCAAAGGGTA TCTAGACACT TATCCGTTAG GACAAACTAC 
ATACAACATG ACAATGGATC AGTTAGTACA AAATTTTCGC TGGTTCCGGG TCCGGGAAAT 
CATTGGATTC GTTATAAGGG TGCTTTTATC CTGATTAAAA GAGAAAGGTC CGCAAAAATG 
ATAGATATCG CAAACGGGTC ACCATTTGAG ACCGTAACCT TAACTACACT GTACCGTGAC 
AAACATTTGT TCGATGACAT TTTGAACGAG GCTAAAGACA TAGCCCTGAA GACTACTGAG 
GGCAAGACTG TTATTTACAC TTCTTTCGGC CCTGAATGGA GAAAGTTTGG TCAACCAAAA 
GCTAAAAGGA TGCTGCCCTC CGTCATTTTG GACAGTGGCA TCAAAGAGGG TATTCTCGAT 
GACGTTTATG ATTTTATGAA GAACGGCAAA TGGTACTCTG ACAGGGGTAT CCCATATCGC 
AGGGGATATT TACTCTATGG CCCTCCCGGA TCTGGTAAGA CTAGCTTTAT CCAGGCTTTA 
GCGGGCGAAT TAGACTACAA CATTTGTATT TTAAACCTCT CCGAAAATAA TTTAACAGAT 
GACAGATTAA ACCACTTAAT GAATAACATG CCAGAAAGGA GCATACTATT ACTGGAAGAT 
ATTGACGCGG CGTTCAACAA AAGAAGTCAA ACTGGTGAAC AAGGTTTTCA TTCTAGCGTA 
ACATTTAGCG GTTTACTGAA TGCCCTGGAT GGCGTCACAT CTTCTGAAGA AACTATTACA 
TTCATGACTA CCAATCATCC TGAAAAGCTA GACGCGGCTA TAATGAGACC AGGTCGTATT 
GACTACAAAG TCTTCGTTGG TAACGCAACT CCATACCAGG TAGAAAAAAT GTTTATGAAA 
TTTTACCCAG GTGAGACTGA TATATGCAAA AAGTTTGTAA ATAGTGTTAA AGAATTGGAT 
ATCACCGTAA GTACCGCCCA GTTGCAAGGA CTTTTCGTTA TGAATAAAGA TGCACCACAC 
GATGCGTTGA AAATGGTCTC CAGCTTACGG AACGCTAATC ATATTTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010663.1
CDS	1..1371
Translation	MSDKPIDIQYDKQATPNLSGVITPPTNETGNDSVREKLSKLVGDAMSNNPYFAAGGGLMILGTGLAVARSGIIKASRVLYRQMIVDLEIQSKDKSYAWFLTWMAKHPQRVSRHLSVRTNYIQHDNGSVSTKFSLVPGPGNHWIRYKGAFILIKRERSAKMIDIANGSPFETVTLTTLYRDKHLFDDILNEAKDIALKTTEGKTVIYTSFGPEWRKFGQPKAKRMLPSVILDSGIKEGILDDVYDFMKNGKWYSDRGIPYRRGYLLYGPPGSGKTSFIQALAGELDYNICILNLSENNLTDDRLNHLMNNMPERSILLLEDIDAAFNKRSQTGEQGFHSSVTFSGLLNALDGVTSSEETITFMTTNHPEKLDAAIMRPGRIDYKVFVGNATPYQVEKMFMKFYPGETDICKKFVNSVKELDITVSTAQLQGLFVMNKDAPHDALKMVSSLRNANHIF

Target ORF information:

RefSeq Version	NM_001180683.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C bifunctional AAA family ATPase chaperone/translocase BCS1 (BCS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180683.1

ORF Insert Sequence:

ATGTCGGATA AGCCGATTGA CATACAATAT GATAAACAGG CTACTCCAAA TCTGAGTGGT 
GTAATTACTC CGCCAACAAA TGAAACTGGT AATGACTCAG TCCGGGAAAA ACTTTCAAAA 
CTAGTTGGCG ATGCTATGTC AAACAATCCC TATTTTGCCG CCGGCGGTGG TCTTATGATC 
CTTGGTACAG GTTTAGCTGT AGCTAGATCC GGTATAATAA AAGCCAGCAG GGTCCTATAC 
CGACAAATGA TTGTCGACTT AGAGATTCAG TCGAAGGACA AATCATACGC CTGGTTTCTA 
ACATGGATGG CCAAACATCC TCAAAGGGTA TCTAGACACT TATCCGTTAG GACAAACTAC 
ATACAACATG ACAATGGATC AGTTAGTACA AAATTTTCGC TGGTTCCGGG TCCGGGAAAT 
CATTGGATTC GTTATAAGGG TGCTTTTATC CTGATTAAAA GAGAAAGGTC CGCAAAAATG 
ATAGATATCG CAAACGGGTC ACCATTTGAG ACCGTAACCT TAACTACACT GTACCGTGAC 
AAACATTTGT TCGATGACAT TTTGAACGAG GCTAAAGACA TAGCCCTGAA GACTACTGAG 
GGCAAGACTG TTATTTACAC TTCTTTCGGC CCTGAATGGA GAAAGTTTGG TCAACCAAAA 
GCTAAAAGGA TGCTGCCCTC CGTCATTTTG GACAGTGGCA TCAAAGAGGG TATTCTCGAT 
GACGTTTATG ATTTTATGAA GAACGGCAAA TGGTACTCTG ACAGGGGTAT CCCATATCGC 
AGGGGATATT TACTCTATGG CCCTCCCGGA TCTGGTAAGA CTAGCTTTAT CCAGGCTTTA 
GCGGGCGAAT TAGACTACAA CATTTGTATT TTAAACCTCT CCGAAAATAA TTTAACAGAT 
GACAGATTAA ACCACTTAAT GAATAACATG CCAGAAAGGA GCATACTATT ACTGGAAGAT 
ATTGACGCGG CGTTCAACAA AAGAAGTCAA ACTGGTGAAC AAGGTTTTCA TTCTAGCGTA 
ACATTTAGCG GTTTACTGAA TGCCCTGGAT GGCGTCACAT CTTCTGAAGA AACTATTACA 
TTCATGACTA CCAATCATCC TGAAAAGCTA GACGCGGCTA TAATGAGACC AGGTCGTATT 
GACTACAAAG TCTTCGTTGG TAACGCAACT CCATACCAGG TAGAAAAAAT GTTTATGAAA 
TTTTACCCAG GTGAGACTGA TATATGCAAA AAGTTTGTAA ATAGTGTTAA AGAATTGGAT 
ATCACCGTAA GTACCGCCCA GTTGCAAGGA CTTTTCGTTA TGAATAAAGA TGCACCACAC 
GATGCGTTGA AAATGGTCTC CAGCTTACGG AACGCTAATC ATATTTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BCS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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