Gene Symbol | VPS33 |
Entrez Gene ID | 851112 |
Full Name | tethering complex ATP-binding subunit VPS33 |
Synonyms | CLS14,MET27,PEP14,SLP1,VAM5,VPL25,VPT33 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » VPS33 cDNA ORF clone
Gene Symbol | VPS33 |
Entrez Gene ID | 851112 |
Full Name | tethering complex ATP-binding subunit VPS33 |
Synonyms | CLS14,MET27,PEP14,SLP1,VAM5,VPL25,VPT33 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001182284.3 | NP_013500.3 | tethering complex ATP-binding subunit VPS33 |
Kluyveromyces lactis | KLLA0B03267g | XP_451679.1 |
Saccharomyces cerevisiae (baker's yeast) | VPS33 | NP_013500.3 |
Eremothecium gossypii | AGOS_AER064C | NP_984924.1 |
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.
Title: A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.
Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes.
Title: The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane.
Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified.
Title: The Habc domain of the SNARE Vam3 interacts with the HOPS tethering complex to facilitate vacuole fusion.
Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes
Title: SM proteins Sly1 and Vps33 co-assemble with Sec17 and SNARE complexes to oppose SNARE disassembly by Sec18.
we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association
Title: Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.
The following VPS33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi00711 | |
Clone ID Related Accession (Same CDS sequence) | NM_001182284.3 | |
Accession Version | NM_001182284.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | tethering complex ATP-binding subunit VPS33 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182284.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGAATAGAT TTTGGAATAC TAAGAAATTT TCATTAACAA ATGCCGATGG ACTATGTGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_013500.3 |
CDS | 1..2076 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001182284.3 |
1 | ATGAATAGAT TTTGGAATAC TAAGAAATTT TCATTAACAA ATGCCGATGG ACTATGTGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. |
Life with 6000 genes. |