BRR2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BRR2
Entrez Gene ID	856919
Full Name	ATP-dependent RNA helicase BRR2
Synonyms	PRP44,RSS1,SLT22,SNU246
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179062.1	NP_011099.1	ATP-dependent RNA helicase BRR2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	brr2	NP_593346.1
Canis lupus familiaris (dog)	SNRNP200	XP_532949.2
Bos taurus (cattle)	SNRNP200	NP_001193092.1
Caenorhabditis elegans (roundworm)	snrp-200	NP_496710.1
Kluyveromyces lactis	KLLA0C17798g	XP_452995.1
Arabidopsis thaliana (thale cress)	AT2G42270	NP_181756.1
Pan troglodytes (chimpanzee)	SNRNP200	XP_001147672.1
Saccharomyces cerevisiae (baker's yeast)	BRR2	NP_011099.1
Eremothecium gossypii	AGOS_AGR113W	NP_986779.1
Neurospora crassa	NCU02685	XP_965458.1
Xenopus tropicalis (tropical clawed frog)	snrnp200	XP_002932581.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001519	XP_321605.3
Magnaporthe oryzae (rice blast fungus)	MGG_15536	XP_003713506.1
Homo sapiens (human)	SNRNP200	NP_054733.2
	SNRNP200	XP_001098299.2
Mus musculus (house mouse)	Snrnp200	NP_796188.2
Rattus norvegicus (Norway rat)	Snrnp200	NP_001032855.2
Danio rerio (zebrafish)	snrnp200	NP_001116729.1
Drosophila melanogaster (fruit fly)	l(3)72Ab	NP_648818.3
Arabidopsis thaliana (thale cress)	emb1507	NP_001185050.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the truncation of the first 247 amino acids of the N terminus in Bad Response to Refrigeration 2 (Delta247-Brr2) weakened RNA binding, reduced helicase activity, and reduced ATPase activity compared with wild type Brr2.
Title: Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity.

the N-terminal region autoinhibits Brr2 via substrate competition and conformational clamping
Title: The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation.

The N-terminal domain iscritical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its interaction with snRNAs and other spliceosomal proteins, revealing a role of Brr2 in spliceosomal activation in addition to U4/U6 unwinding.
Title: Brr2 plays a role in spliceosomal activation in addition to U4/U6 unwinding.

Sad1 maintains the integrity of the tri-snRNP by counteracting Brr2-mediated dissociation of tri-snRNP.
Title: Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis.

We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP.
Title: The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.

The following BRR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05667	NM_001179062.1 Latest version!	Saccharomyces cerevisiae S288C ATP-dependent RNA helicase BRR2 (BRR2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRR2 ( NM_001179062.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011099.1 Saccharomyces cerevisiae S288C ATP-dependent RNA helicase BRR2 (BRR2), partial mRNA.

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CloneID	OSi05667
Clone ID Related Accession (Same CDS sequence)	NM_001179062.1
Accession Version	NM_001179062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-dependent RNA helicase BRR2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGAGC ATGAAACGAA GGATAAGGCC AAAAAAATTA GAGAAATTTA TCGTTATGAT 
GAGATGTCCA ATAAGGTGTT GAAAGTTGAT AAACGCTTTA TGAATACCAG CCAAAATCCT 
CAAAGGGATG CGGAAATATC ACAACCAAAA TCCATGAGTG GTAGAATTAG TGCTAAGGAT 
ATGGGACAGG GCCTGTGCAA TAACATTAAT AAAGGACTAA AAGAAAATGA TGTAGCCGTT 
GAAAAAACTG GAAAGAGCGC ATCATTGAAA AAAATTCAGC AGCATAACAC CATTTTGAAT 
TCTTCCTCCG ATTTCAGGTT ACATTATTAT CCAAAGGATC CCTCAAATGT CGAAACTTAC 
GAGCAGATTT TGCAGTGGGT TACAGAAGTT TTAGGCAATG ATATACCCCA TGATCTTATT 
ATTGGAACTG CTGATATATT CATCAGACAA TTGAAAGAAA ATGAGGAAAA TGAGGATGGT 
AATATTGAAG AGCGAAAGGA AAAGATCCAG CATGAGCTAG GCATCAATAT AGACTCCCTG 
AAGTTTAACG AATTAGTTAA ACTTATGAAA AACATTACCG ATTATGAAAC ACATCCTGAC 
AATAGCAATA AACAAGCAGT AGCAATTCTT GCCGATGATG AAAAATCAGA TGAAGAAGAA 
GTAACAGAAA TGTCCAACAA TGCTAATGTT CTTGGGGGAG AAATCAATGA TAATGAAGAT 
GATGATGAGG AATATGATTA CAATGACGTT GAAGTCAATT CTAAAAAAAA AAACAAGAGG 
GCATTGCCAA ACATTGAAAA CGATATAATT AAACTCTCCG ATAGTAAAAC AAGTAATATC 
GAGAGCGTTC CGATATACTC AATCGATGAA TTTTTTTTGC AGAGAAAACT TCGGTCAGAG 
CTTGGTTACA AGGATACCTC TGTAATACAA GACCTGTCAG AAAAAATTTT GAATGATATT 
GAAACACTTG AGCATAATCC AGTAGCGTTG GAGCAGAAGT TGGTTGATCT GCTTAAATTC 
GAGAATATAT CACTGGCTGA ATTTATTCTC AAGAATAGAT CTACAATTTT TTGGGGCATA 
CGACTGGCGA AGAGCACAGA GAATGAAATA CCGAACTTAA TAGAGAAAAT GGTTGCAAAA 
GGTTTGAATG ATCTTGTGGA ACAGTATAAA TTTAGAGAAA CTACACACTC AAAGCGTGAA 
CTCGATTCCG GTGACGACCA GCCACAGTCT TCGGAAGCAA AAAGAACAAA ATTCAGTAAT 
CCAGCAATAC CGCCCGTCAT AGATTTGGAA AAGATTAAAT TCGACGAAAG TTCTAAATTA 
ATGACCGTGA CAAAAGTATC CCTACCGGAA GGATCATTCA AAAGGGTGAA ACCACAATAT 
GACGAAATAC ATATTCCAGC TCCTAGCAAA CCTGTTATTG ATTATGAGTT GAAAGAAATT 
ACTTCTCTAC CAGATTGGTG TCAGGAGGCG TTTCCTTCAT CGGAAACAAC GTCCTTAAAC 
CCTATACAAT CAAAGGTTTT TCACGCAGCA TTTGAAGGTG ATTCAAACAT GCTAATATGT 
GCACCCACTG GTTCAGGTAA GACGAATATC GCGTTATTGA CGGTATTGAA AGCCTTATCC 
CATCATTATA ACCCGAAAAC TAAGAAATTA AACCTTTCTG CATTTAAAAT TGTTTATATA 
GCACCCTTGA AAGCCTTAGT ACAGGAACAA GTCCGAGAAT TTCAAAGGAG GTTGGCTTTT 
CTAGGTATAA AAGTCGCTGA ATTGACTGGT GATTCTAGGC TTAGTAGAAA GCAAATTGAC 
GAGACGCAGG TTTTGGTGTC TACGCCAGAG AAATGGGACA TAACTACTAG AAATTCAAAT 
AATTTGGCCA TCGTAGAACT TGTTCGTTTG TTGATTATAG ATGAAATACA TTTGTTACAT 
GATGACAGGG GACCAGTATT GGAAAGCATC GTGGCTAGGA CATTTTGGGC TTCCAAATAT 
GGCCAGGAAT ACCCCAGAAT AATAGGCCTT TCTGCCACCT TACCAAATTA TGAGGATGTC 
GGTAGGTTTT TAAGAGTTCC GAAGGAAGGA TTGTTTTATT TTGACTCCTC ATTTAGACCG 
TGTCCTCTAT CTCAACAGTT TTGTGGCATT AAGGAACGGA ACTCCTTGAA AAAGCTGAAG 
GCTATGAATG ATGCTTGTTA TGAAAAGGTA TTGGAATCCA TCAATGAAGG TAATCAAATA 
ATCGTGTTTG TTCATTCTCG AAAAGAAACA TCACGAACAG CAACATGGTT GAAAAATAAA 
TTTGCTGAAG AGAACATTAC TCATAAATTA ACCAAAAATG ATGCAGGATC GAAGCAGATT 
TTAAAGACAG AAGCAGCCAA CGTACTCGAT CCAAGTCTAC GAAAGTTGAT TGAAAGCGGT 
ATAGGGACGC ATCACGCAGG GCTGACCAGA AGTGACAGAT CCTTATCTGA AGATTTATTT 
GCAGACGGGT TGTTGCAAGT ATTGGTATGC ACTGCGACGT TAGCATGGGG TGTTAATCTA 
CCTGCTCATA CAGTGATTAT AAAAGGAACA GATGTCTATT CACCTGAAAA GGGGTCCTGG 
GAACAACTTT CTCCACAAGA TGTACTTCAA ATGTTAGGTA GAGCAGGCAG ACCGAGATAT 
GACACATTTG GCGAGGGTAT TATAATAACA GACCAATCGA ATGTTCAATA TTATCTCTCG 
GTTTTAAATC AACAATTACC AATAGAATCG CAATTTGTTT CAAAATTAGT TGACAACTTA 
AATGCAGAAG TTGTCGCGGG TAACATTAAG TGCAGGAATG ATGCTGTGAA TTGGTTGGCG 
TATACTTACC TATATGTCAG AATGTTAGCT TCCCCTATGC TTTACAAAGT ACCTGATATT 
TCAAGTGATG GGCAATTGAA AAAGTTTAGA GAAAGCTTAG TCCATTCGGC CCTATGCATT 
TTAAAAGAAC AAGAGTTGGT GTTATATGAT GCTGAAAATG ATGTAATTGA GGCGACCGAT 
CTAGGAAATA TAGCGTCGTC ATTTTACATA AATCATGCTT CCATGGATGT TTACAATAGA 
GAGTTAGATG AACATACAAC TCAGATTGAT CTTTTTAGAA TATTCTCAAT GTCGGAAGAG 
TTTAAGTACG TATCGGTGAG ATATGAAGAG AAAAGAGAAC TAAAACAACT TCTAGAGAAA 
GCACCCATTC CTATCAGAGA AGATATTGAT GATCCTTTAG CAAAAGTGAA TGTACTACTG 
CAGTCTTATT TTTCCCAGCT AAAGTTCGAA GGTTTTGCAC TTAATTCTGA TATAGTGTTT 
ATTCACCAAA ATGCTGGTAG GTTATTGCGT GCTATGTTTG AAATATGCTT GAAGAGAGGA 
TGGGGACATC CCACACGGAT GTTGTTAAAT CTTTGCAAAT CTGCCACAAC TAAAATGTGG 
CCAACAAACT GTCCTTTAAG ACAGTTTAAA ACATGCCCTG TCGAAGTGAT TAAAAGGCTG 
GAAGCCTCTA CAGTACCGTG GGGAGATTAT CTACAGCTAG AAACACCTGC CGAAGTTGGT 
CGTGCAATCA GATCTGAAAA ATATGGAAAA CAGGTTTACG ATTTATTGAA AAGATTTCCC 
AAAATGTCCG TAACATGCAA CGCTCAGCCA ATTACCCGTA GCGTTATGAG GTTCAACATT 
GAAATAATAG CCGACTGGAT ATGGGATATG AATGTACACG GTTCCCTTGA ACCTTTTTTG 
CTCATGCTAG AAGACACTGA TGGTGATTCA ATACTTTATT ATGACGTACT ATTCATCACT 
CCAGATATAG TTGGTCATGA ATTTACCTTA TCCTTTACTT ATGAGTTAAA GCAGCACAAT 
CAAAATAACT TACCACCTAA TTTCTTCCTT ACTTTGATAT CAGAAAATTG GTGGCACAGT 
GAATTCGAGA TTCCAGTTTC CTTCAATGGA TTTAAACTTC CGAAAAAATT TCCTCCTCCT 
ACTCCCCTTC TGGAGAATAT TAGTATTTCA ACATCAGAGC TTGGCAATGA TGATTTTTCC 
GAAGTATTTG AATTTAAAAC TTTCAACAAG ATTCAGAGCC AGGTCTTCGA GTCTCTCTAC 
AATTCGAATG ACTCGGTCTT TGTAGGTTCA GGAAAGGGCA CAGGTAAAAC AGCTATGGCT 
GAATTAGCAC TGTTAAATCA TTGGAGACAG AATAAAGGAA GAGCGGTTTA TATCAATCCG 
TCTGGGGAAA AAATTGATTT TTTGCTATCT GATTGGAATA AAAGGTTCTC TCATCTTGCT 
GGTGGTAAGA TAATAAATAA GCTAGGAAAT GATCCTTCAC TAAATTTAAA ATTATTAGCA 
AAAAGCCATG TTCTCTTGGC TACCCCCGTG CAATTTGAGC TTTTGTCACG TCGTTGGAGA 
CAAAGGAAGA ACATCCAAAG TTTAGAATTA ATGATATATG ATGACGCTCA TGAGATTAGC 
CAAGGTGTTT ACGGTGCTGT GTATGAAACG CTAATTTCTA GAATGATATT TATAGCAACC 
CAACTTGAAA AGAAAATTCG ATTTGTTTGC CTTTCGAACT GTTTGGCAAA TGCTCGTGAT 
TTTGGTGAAT GGGCTGGTAT GACGAAGTCA AACATCTACA ACTTTTCTCC AAGCGAAAGA 
ATTGAGCCTC TTGAAATTAA TATTCAGTCA TTTAAAGATG TTGAGCACAT ATCATTCAAT 
TTTTCGATGC TTCAAATGGC ATTTGAAGCA TCTGCTGCTG CAGCGGGAAA TCGTAATTCC 
AGTTCGGTGT TCTTACCATC TAGAAAGGAT TGTATGGAAG TTGCGTCTGC ATTTATGAAG 
TTTTCAAAAG CAATTGAATG GGATATGTTG AACGTTGAAG AAGAGCAAAT TGTACCATAC 
ATTGAAAAGT TAACAGATGG TCATCTAAGA GCACCTTTAA AACACGGTGT TGGTATTTTG 
TATAAGGGGA TGGCATCAAA TGATGAAAGA ATTGTGAAAA GGTTATACGA ATATGGTGCG 
GTATCGGTTT TACTCATTTC TAAGGATTGT TCTGCATTCG CTTGTAAAAC TGATGAAGTG 
ATTATTTTAG GAACCAACCT TTATGATGGT GCAGAGCATA AATATATGCC ATATACCATA 
AATGAACTTC TAGAAATGGT GGGACTAGCT AGTGGTAACG ATTCAATGGC AGGCAAGGTT 
TTAATTTTGA CAAGTCACAA CATGAAGGCC TACTACAAAA AATTCTTAAT TGAGCCTTTG 
CCTACTGAGA GTTACCTACA GTATATTATT CATGATACTC TGAACAACGA GATAGCAAAT 
TCTATTATAC AAAGCAAACA AGATTGTGTT GACTGGTTTA CATATTCGTA TTTCTATCGT 
CGTATCCATG TGAATCCAAG CTATTATGGC GTAAGGGATA CGTCTCCACA TGGAATTTCA 
GTTTTTTTGA GTAATTTAGT TGAAACTTGT TTGAATGACT TAGTTGAATC ATCCTTCATT 
GAAATTGACG ATACAGAAGC TGAAGTAACC GCAGAAGTTA ATGGAGGAGA TGATGAAGCA 
ACTGAAATCA TTTCTACCTT GAGCAACGGG CTAATTGCTT CTCACTATGG CGTATCATTT 
TTTACTATTC AGTCATTCGT TTCTTCTTTA TCGAATACGT CAACGCTCAA AAACATGCTG 
TATGTTCTTT CAACAGCAGT AGAATTCGAA AGTGTTCCAC TCAGAAAAGG AGACAGAGCA 
TTACTTGTCA AATTAAGTAA AAGACTACCA TTGAGATTCC CTGAACACAC CTCTTCTGGT 
TCGGTGAGCT TTAAAGTTTT CTTATTGTTA CAAGCATATT TTTCACGTCT TGAATTACCT 
GTCGACTTTC AAAATGATTT AAAAGACATT CTAGAAAAGG TTGTTCCACT AATTAACGTA 
GTGGTTGATA TCCTTTCAGC AAACGGGTAT TTGAATGCTA CTACCGCCAT GGATTTGGCG 
CAGATGTTGA TTCAGGGTGT ATGGGACGTT GATAATCCGT TGAGACAGAT TCCACACTTC 
AACAACAAAA TTTTGGAAAA ATGTAAAGAA ATTAACGTCG AGACGGTCTA TGATATAATG 
GCCCTTGAGG ATGAAGAGAG GGATGAAATT TTAACGCTTA CTGATTCACA GCTTGCGCAG 
GTCGCCGCGT TTGTTAACAA TTACCCCAAC GTTGAACTTA CTTATTCGTT GAATAATTCT 
GATTCGTTAA TTTCAGGTGT AAAACAGAAA ATTACCATTC AATTGACAAG AGATGTTGAA 
CCTGAGAACT TACAAGTAAC ATCTGAAAAG TATCCGTTTG ATAAGCTAGA AAGTTGGTGG 
TTGGTTTTAG GTGAAGTATC TAAAAAAGAG CTTTATGCGA TCAAGAAAGT TACTTTAAAT 
AAAGAGACTC AGCAATATGA ATTGGAATTT GACACTCCGA CATCTGGTAA ACATAACTTA 
ACAATTTGGT GTGTCTGTGA TTCATATCTT GACGCAGATA AAGAGTTGTC CTTTGAAATA 
AATGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011099.1
CDS	1..6492
Translation	MTEHETKDKAKKIREIYRYDEMSNKVLKVDKRFMNTSQNPQRDAEISQPKSMSGRISAKDMGQGLCNNINKGLKENDVAVEKTGKSASLKKIQQHNTILNSSSDFRLHYYPKDPSNVETYEQILQWVTEVLGNDIPHDLIIGTADIFIRQLKENEENEDGNIEERKEKIQHELGINIDSLKFNELVKLMKNITDYETHPDNSNKQAVAILADDEKSDEEEVTEMSNNANVLGGEINDNEDDDEEYDYNDVEVNSKKKNKRALPNIENDIIKLSDSKTSNIESVPIYSIDEFFLQRKLRSELGYKDTSVIQDLSEKILNDIETLEHNPVALEQKLVDLLKFENISLAEFILKNRSTIFWGIRLAKSTENEIPNLIEKMVAKGLNDLVEQYKFRETTHSKRELDSGDDQPQSSEAKRTKFSNPAIPPVIDLEKIKFDESSKLMTVTKVSLPEGSFKRVKPQYDEIHIPAPSKPVIDYELKEITSLPDWCQEAFPSSETTSLNPIQSKVFHAAFEGDSNMLICAPTGSGKTNIALLTVLKALSHHYNPKTKKLNLSAFKIVYIAPLKALVQEQVREFQRRLAFLGIKVAELTGDSRLSRKQIDETQVLVSTPEKWDITTRNSNNLAIVELVRLLIIDEIHLLHDDRGPVLESIVARTFWASKYGQEYPRIIGLSATLPNYEDVGRFLRVPKEGLFYFDSSFRPCPLSQQFCGIKERNSLKKLKAMNDACYEKVLESINEGNQIIVFVHSRKETSRTATWLKNKFAEENITHKLTKNDAGSKQILKTEAANVLDPSLRKLIESGIGTHHAGLTRSDRSLSEDLFADGLLQVLVCTATLAWGVNLPAHTVIIKGTDVYSPEKGSWEQLSPQDVLQMLGRAGRPRYDTFGEGIIITDQSNVQYYLSVLNQQLPIESQFVSKLVDNLNAEVVAGNIKCRNDAVNWLAYTYLYVRMLASPMLYKVPDISSDGQLKKFRESLVHSALCILKEQELVLYDAENDVIEATDLGNIASSFYINHASMDVYNRELDEHTTQIDLFRIFSMSEEFKYVSVRYEEKRELKQLLEKAPIPIREDIDDPLAKVNVLLQSYFSQLKFEGFALNSDIVFIHQNAGRLLRAMFEICLKRGWGHPTRMLLNLCKSATTKMWPTNCPLRQFKTCPVEVIKRLEASTVPWGDYLQLETPAEVGRAIRSEKYGKQVYDLLKRFPKMSVTCNAQPITRSVMRFNIEIIADWIWDMNVHGSLEPFLLMLEDTDGDSILYYDVLFITPDIVGHEFTLSFTYELKQHNQNNLPPNFFLTLISENWWHSEFEIPVSFNGFKLPKKFPPPTPLLENISISTSELGNDDFSEVFEFKTFNKIQSQVFESLYNSNDSVFVGSGKGTGKTAMAELALLNHWRQNKGRAVYINPSGEKIDFLLSDWNKRFSHLAGGKIINKLGNDPSLNLKLLAKSHVLLATPVQFELLSRRWRQRKNIQSLELMIYDDAHEISQGVYGAVYETLISRMIFIATQLEKKIRFVCLSNCLANARDFGEWAGMTKSNIYNFSPSERIEPLEINIQSFKDVEHISFNFSMLQMAFEASAAAAGNRNSSSVFLPSRKDCMEVASAFMKFSKAIEWDMLNVEEEQIVPYIEKLTDGHLRAPLKHGVGILYKGMASNDERIVKRLYEYGAVSVLLISKDCSAFACKTDEVIILGTNLYDGAEHKYMPYTINELLEMVGLASGNDSMAGKVLILTSHNMKAYYKKFLIEPLPTESYLQYIIHDTLNNEIANSIIQSKQDCVDWFTYSYFYRRIHVNPSYYGVRDTSPHGISVFLSNLVETCLNDLVESSFIEIDDTEAEVTAEVNGGDDEATEIISTLSNGLIASHYGVSFFTIQSFVSSLSNTSTLKNMLYVLSTAVEFESVPLRKGDRALLVKLSKRLPLRFPEHTSSGSVSFKVFLLLQAYFSRLELPVDFQNDLKDILEKVVPLINVVVDILSANGYLNATTAMDLAQMLIQGVWDVDNPLRQIPHFNNKILEKCKEINVETVYDIMALEDEERDEILTLTDSQLAQVAAFVNNYPNVELTYSLNNSDSLISGVKQKITIQLTRDVEPENLQVTSEKYPFDKLESWWLVLGEVSKKELYAIKKVTLNKETQQYELEFDTPTSGKHNLTIWCVCDSYLDADKELSFEINVK

Target ORF information:

RefSeq Version	NM_001179062.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-dependent RNA helicase BRR2 (BRR2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179062.1

ORF Insert Sequence:

ATGACTGAGC ATGAAACGAA GGATAAGGCC AAAAAAATTA GAGAAATTTA TCGTTATGAT 
GAGATGTCCA ATAAGGTGTT GAAAGTTGAT AAACGCTTTA TGAATACCAG CCAAAATCCT 
CAAAGGGATG CGGAAATATC ACAACCAAAA TCCATGAGTG GTAGAATTAG TGCTAAGGAT 
ATGGGACAGG GCCTGTGCAA TAACATTAAT AAAGGACTAA AAGAAAATGA TGTAGCCGTT 
GAAAAAACTG GAAAGAGCGC ATCATTGAAA AAAATTCAGC AGCATAACAC CATTTTGAAT 
TCTTCCTCCG ATTTCAGGTT ACATTATTAT CCAAAGGATC CCTCAAATGT CGAAACTTAC 
GAGCAGATTT TGCAGTGGGT TACAGAAGTT TTAGGCAATG ATATACCCCA TGATCTTATT 
ATTGGAACTG CTGATATATT CATCAGACAA TTGAAAGAAA ATGAGGAAAA TGAGGATGGT 
AATATTGAAG AGCGAAAGGA AAAGATCCAG CATGAGCTAG GCATCAATAT AGACTCCCTG 
AAGTTTAACG AATTAGTTAA ACTTATGAAA AACATTACCG ATTATGAAAC ACATCCTGAC 
AATAGCAATA AACAAGCAGT AGCAATTCTT GCCGATGATG AAAAATCAGA TGAAGAAGAA 
GTAACAGAAA TGTCCAACAA TGCTAATGTT CTTGGGGGAG AAATCAATGA TAATGAAGAT 
GATGATGAGG AATATGATTA CAATGACGTT GAAGTCAATT CTAAAAAAAA AAACAAGAGG 
GCATTGCCAA ACATTGAAAA CGATATAATT AAACTCTCCG ATAGTAAAAC AAGTAATATC 
GAGAGCGTTC CGATATACTC AATCGATGAA TTTTTTTTGC AGAGAAAACT TCGGTCAGAG 
CTTGGTTACA AGGATACCTC TGTAATACAA GACCTGTCAG AAAAAATTTT GAATGATATT 
GAAACACTTG AGCATAATCC AGTAGCGTTG GAGCAGAAGT TGGTTGATCT GCTTAAATTC 
GAGAATATAT CACTGGCTGA ATTTATTCTC AAGAATAGAT CTACAATTTT TTGGGGCATA 
CGACTGGCGA AGAGCACAGA GAATGAAATA CCGAACTTAA TAGAGAAAAT GGTTGCAAAA 
GGTTTGAATG ATCTTGTGGA ACAGTATAAA TTTAGAGAAA CTACACACTC AAAGCGTGAA 
CTCGATTCCG GTGACGACCA GCCACAGTCT TCGGAAGCAA AAAGAACAAA ATTCAGTAAT 
CCAGCAATAC CGCCCGTCAT AGATTTGGAA AAGATTAAAT TCGACGAAAG TTCTAAATTA 
ATGACCGTGA CAAAAGTATC CCTACCGGAA GGATCATTCA AAAGGGTGAA ACCACAATAT 
GACGAAATAC ATATTCCAGC TCCTAGCAAA CCTGTTATTG ATTATGAGTT GAAAGAAATT 
ACTTCTCTAC CAGATTGGTG TCAGGAGGCG TTTCCTTCAT CGGAAACAAC GTCCTTAAAC 
CCTATACAAT CAAAGGTTTT TCACGCAGCA TTTGAAGGTG ATTCAAACAT GCTAATATGT 
GCACCCACTG GTTCAGGTAA GACGAATATC GCGTTATTGA CGGTATTGAA AGCCTTATCC 
CATCATTATA ACCCGAAAAC TAAGAAATTA AACCTTTCTG CATTTAAAAT TGTTTATATA 
GCACCCTTGA AAGCCTTAGT ACAGGAACAA GTCCGAGAAT TTCAAAGGAG GTTGGCTTTT 
CTAGGTATAA AAGTCGCTGA ATTGACTGGT GATTCTAGGC TTAGTAGAAA GCAAATTGAC 
GAGACGCAGG TTTTGGTGTC TACGCCAGAG AAATGGGACA TAACTACTAG AAATTCAAAT 
AATTTGGCCA TCGTAGAACT TGTTCGTTTG TTGATTATAG ATGAAATACA TTTGTTACAT 
GATGACAGGG GACCAGTATT GGAAAGCATC GTGGCTAGGA CATTTTGGGC TTCCAAATAT 
GGCCAGGAAT ACCCCAGAAT AATAGGCCTT TCTGCCACCT TACCAAATTA TGAGGATGTC 
GGTAGGTTTT TAAGAGTTCC GAAGGAAGGA TTGTTTTATT TTGACTCCTC ATTTAGACCG 
TGTCCTCTAT CTCAACAGTT TTGTGGCATT AAGGAACGGA ACTCCTTGAA AAAGCTGAAG 
GCTATGAATG ATGCTTGTTA TGAAAAGGTA TTGGAATCCA TCAATGAAGG TAATCAAATA 
ATCGTGTTTG TTCATTCTCG AAAAGAAACA TCACGAACAG CAACATGGTT GAAAAATAAA 
TTTGCTGAAG AGAACATTAC TCATAAATTA ACCAAAAATG ATGCAGGATC GAAGCAGATT 
TTAAAGACAG AAGCAGCCAA CGTACTCGAT CCAAGTCTAC GAAAGTTGAT TGAAAGCGGT 
ATAGGGACGC ATCACGCAGG GCTGACCAGA AGTGACAGAT CCTTATCTGA AGATTTATTT 
GCAGACGGGT TGTTGCAAGT ATTGGTATGC ACTGCGACGT TAGCATGGGG TGTTAATCTA 
CCTGCTCATA CAGTGATTAT AAAAGGAACA GATGTCTATT CACCTGAAAA GGGGTCCTGG 
GAACAACTTT CTCCACAAGA TGTACTTCAA ATGTTAGGTA GAGCAGGCAG ACCGAGATAT 
GACACATTTG GCGAGGGTAT TATAATAACA GACCAATCGA ATGTTCAATA TTATCTCTCG 
GTTTTAAATC AACAATTACC AATAGAATCG CAATTTGTTT CAAAATTAGT TGACAACTTA 
AATGCAGAAG TTGTCGCGGG TAACATTAAG TGCAGGAATG ATGCTGTGAA TTGGTTGGCG 
TATACTTACC TATATGTCAG AATGTTAGCT TCCCCTATGC TTTACAAAGT ACCTGATATT 
TCAAGTGATG GGCAATTGAA AAAGTTTAGA GAAAGCTTAG TCCATTCGGC CCTATGCATT 
TTAAAAGAAC AAGAGTTGGT GTTATATGAT GCTGAAAATG ATGTAATTGA GGCGACCGAT 
CTAGGAAATA TAGCGTCGTC ATTTTACATA AATCATGCTT CCATGGATGT TTACAATAGA 
GAGTTAGATG AACATACAAC TCAGATTGAT CTTTTTAGAA TATTCTCAAT GTCGGAAGAG 
TTTAAGTACG TATCGGTGAG ATATGAAGAG AAAAGAGAAC TAAAACAACT TCTAGAGAAA 
GCACCCATTC CTATCAGAGA AGATATTGAT GATCCTTTAG CAAAAGTGAA TGTACTACTG 
CAGTCTTATT TTTCCCAGCT AAAGTTCGAA GGTTTTGCAC TTAATTCTGA TATAGTGTTT 
ATTCACCAAA ATGCTGGTAG GTTATTGCGT GCTATGTTTG AAATATGCTT GAAGAGAGGA 
TGGGGACATC CCACACGGAT GTTGTTAAAT CTTTGCAAAT CTGCCACAAC TAAAATGTGG 
CCAACAAACT GTCCTTTAAG ACAGTTTAAA ACATGCCCTG TCGAAGTGAT TAAAAGGCTG 
GAAGCCTCTA CAGTACCGTG GGGAGATTAT CTACAGCTAG AAACACCTGC CGAAGTTGGT 
CGTGCAATCA GATCTGAAAA ATATGGAAAA CAGGTTTACG ATTTATTGAA AAGATTTCCC 
AAAATGTCCG TAACATGCAA CGCTCAGCCA ATTACCCGTA GCGTTATGAG GTTCAACATT 
GAAATAATAG CCGACTGGAT ATGGGATATG AATGTACACG GTTCCCTTGA ACCTTTTTTG 
CTCATGCTAG AAGACACTGA TGGTGATTCA ATACTTTATT ATGACGTACT ATTCATCACT 
CCAGATATAG TTGGTCATGA ATTTACCTTA TCCTTTACTT ATGAGTTAAA GCAGCACAAT 
CAAAATAACT TACCACCTAA TTTCTTCCTT ACTTTGATAT CAGAAAATTG GTGGCACAGT 
GAATTCGAGA TTCCAGTTTC CTTCAATGGA TTTAAACTTC CGAAAAAATT TCCTCCTCCT 
ACTCCCCTTC TGGAGAATAT TAGTATTTCA ACATCAGAGC TTGGCAATGA TGATTTTTCC 
GAAGTATTTG AATTTAAAAC TTTCAACAAG ATTCAGAGCC AGGTCTTCGA GTCTCTCTAC 
AATTCGAATG ACTCGGTCTT TGTAGGTTCA GGAAAGGGCA CAGGTAAAAC AGCTATGGCT 
GAATTAGCAC TGTTAAATCA TTGGAGACAG AATAAAGGAA GAGCGGTTTA TATCAATCCG 
TCTGGGGAAA AAATTGATTT TTTGCTATCT GATTGGAATA AAAGGTTCTC TCATCTTGCT 
GGTGGTAAGA TAATAAATAA GCTAGGAAAT GATCCTTCAC TAAATTTAAA ATTATTAGCA 
AAAAGCCATG TTCTCTTGGC TACCCCCGTG CAATTTGAGC TTTTGTCACG TCGTTGGAGA 
CAAAGGAAGA ACATCCAAAG TTTAGAATTA ATGATATATG ATGACGCTCA TGAGATTAGC 
CAAGGTGTTT ACGGTGCTGT GTATGAAACG CTAATTTCTA GAATGATATT TATAGCAACC 
CAACTTGAAA AGAAAATTCG ATTTGTTTGC CTTTCGAACT GTTTGGCAAA TGCTCGTGAT 
TTTGGTGAAT GGGCTGGTAT GACGAAGTCA AACATCTACA ACTTTTCTCC AAGCGAAAGA 
ATTGAGCCTC TTGAAATTAA TATTCAGTCA TTTAAAGATG TTGAGCACAT ATCATTCAAT 
TTTTCGATGC TTCAAATGGC ATTTGAAGCA TCTGCTGCTG CAGCGGGAAA TCGTAATTCC 
AGTTCGGTGT TCTTACCATC TAGAAAGGAT TGTATGGAAG TTGCGTCTGC ATTTATGAAG 
TTTTCAAAAG CAATTGAATG GGATATGTTG AACGTTGAAG AAGAGCAAAT TGTACCATAC 
ATTGAAAAGT TAACAGATGG TCATCTAAGA GCACCTTTAA AACACGGTGT TGGTATTTTG 
TATAAGGGGA TGGCATCAAA TGATGAAAGA ATTGTGAAAA GGTTATACGA ATATGGTGCG 
GTATCGGTTT TACTCATTTC TAAGGATTGT TCTGCATTCG CTTGTAAAAC TGATGAAGTG 
ATTATTTTAG GAACCAACCT TTATGATGGT GCAGAGCATA AATATATGCC ATATACCATA 
AATGAACTTC TAGAAATGGT GGGACTAGCT AGTGGTAACG ATTCAATGGC AGGCAAGGTT 
TTAATTTTGA CAAGTCACAA CATGAAGGCC TACTACAAAA AATTCTTAAT TGAGCCTTTG 
CCTACTGAGA GTTACCTACA GTATATTATT CATGATACTC TGAACAACGA GATAGCAAAT 
TCTATTATAC AAAGCAAACA AGATTGTGTT GACTGGTTTA CATATTCGTA TTTCTATCGT 
CGTATCCATG TGAATCCAAG CTATTATGGC GTAAGGGATA CGTCTCCACA TGGAATTTCA 
GTTTTTTTGA GTAATTTAGT TGAAACTTGT TTGAATGACT TAGTTGAATC ATCCTTCATT 
GAAATTGACG ATACAGAAGC TGAAGTAACC GCAGAAGTTA ATGGAGGAGA TGATGAAGCA 
ACTGAAATCA TTTCTACCTT GAGCAACGGG CTAATTGCTT CTCACTATGG CGTATCATTT 
TTTACTATTC AGTCATTCGT TTCTTCTTTA TCGAATACGT CAACGCTCAA AAACATGCTG 
TATGTTCTTT CAACAGCAGT AGAATTCGAA AGTGTTCCAC TCAGAAAAGG AGACAGAGCA 
TTACTTGTCA AATTAAGTAA AAGACTACCA TTGAGATTCC CTGAACACAC CTCTTCTGGT 
TCGGTGAGCT TTAAAGTTTT CTTATTGTTA CAAGCATATT TTTCACGTCT TGAATTACCT 
GTCGACTTTC AAAATGATTT AAAAGACATT CTAGAAAAGG TTGTTCCACT AATTAACGTA 
GTGGTTGATA TCCTTTCAGC AAACGGGTAT TTGAATGCTA CTACCGCCAT GGATTTGGCG 
CAGATGTTGA TTCAGGGTGT ATGGGACGTT GATAATCCGT TGAGACAGAT TCCACACTTC 
AACAACAAAA TTTTGGAAAA ATGTAAAGAA ATTAACGTCG AGACGGTCTA TGATATAATG 
GCCCTTGAGG ATGAAGAGAG GGATGAAATT TTAACGCTTA CTGATTCACA GCTTGCGCAG 
GTCGCCGCGT TTGTTAACAA TTACCCCAAC GTTGAACTTA CTTATTCGTT GAATAATTCT 
GATTCGTTAA TTTCAGGTGT AAAACAGAAA ATTACCATTC AATTGACAAG AGATGTTGAA 
CCTGAGAACT TACAAGTAAC ATCTGAAAAG TATCCGTTTG ATAAGCTAGA AAGTTGGTGG 
TTGGTTTTAG GTGAAGTATC TAAAAAAGAG CTTTATGCGA TCAAGAAAGT TACTTTAAAT 
AAAGAGACTC AGCAATATGA ATTGGAATTT GACACTCCGA CATCTGGTAA ACATAACTTA 
ACAATTTGGT GTGTCTGTGA TTCATATCTT GACGCAGATA AAGAGTTGTC CTTTGAAATA 
AATGTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BRR2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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