MYO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO1
Entrez Gene ID	856418
Full Name	myosin 1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179153.1	NP_011888.1	myosin 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	smyhc1	NP_001018343.1
Danio rerio (zebrafish)	vmhcl	NP_001070932.2
Drosophila melanogaster (fruit fly)	Mhc	NP_001246049.1
Rattus norvegicus (Norway rat)	Myh7	NP_058936.1
Danio rerio (zebrafish)	LOC100329748	XP_002666778.1
Eremothecium gossypii	AGOS_ACR068W	NP_983470.2
Homo sapiens (human)	MYH7	NP_000248.2
Canis lupus familiaris (dog)	MYH7	NP_001107183.1
Mus musculus (house mouse)	Myh7	NP_542766.1
Danio rerio (zebrafish)	vmhc	NP_001106204.1
Danio rerio (zebrafish)	si:ch211-24n20.3	XP_001923213.1
Danio rerio (zebrafish)	smyhc2	NP_001096096.2
Danio rerio (zebrafish)	LOC100329813	XP_002666779.1
Saccharomyces cerevisiae (baker's yeast)	MYO1	NP_011888.1
Kluyveromyces lactis	KLLA0E11595g	XP_454473.1
Pan troglodytes (chimpanzee)	MYH7	XP_001150786.3
Bos taurus (cattle)	MYH7	NP_777152.1
Danio rerio (zebrafish)	LOC100329670	XP_002666777.2
Danio rerio (zebrafish)	LOC567740	XP_696132.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010147	XP_319309.3
Xenopus tropicalis (tropical clawed frog)	myh7	XP_002939061.1
Gallus gallus (chicken)	MYH7	NP_001001302.1
Danio rerio (zebrafish)	smyhc3	NP_001129995.1
Schizosaccharomyces pombe (fission yeast)	myo2	NP_588114.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

myosin-mediated coupling between actin filaments and the base of endocytic sites is essential for allowing actin polymerization to drive membrane invagination
Title: An Engineered Minimal WASP-Myosin Fusion Protein Reveals Essential Functions for Endocytosis.

Myo1 plays a major role in the septin-dependent Mlc1 localization to the division site before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament-dependent Mlc1 localization during cytokinesis.
Title: Myosin?II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.

Deletion of MYO1 impairs the cluster formation of Ire1.
Title: F-actin and a type-II myosin are required for efficient clustering of the ER stress sensor Ire1.

Yeast myosin-II (Myo1p) and myosin-V (Myo2p) accommodate the reduced N-terminal charge density of yeast actin, showing greater activity with yeast over muscle actin
Title: Functional adaptation between yeast actin and its cognate myosin motors.

Results establish a biphasic targeting mechanism for Myo1 and highlights the role of the contractile actomyosin ring in cytokinesis beyond force generation.
Title: Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II.

The following MYO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05221	NM_001179153.1 Latest version!	Saccharomyces cerevisiae S288C myosin 1 (MYO1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO1 ( NM_001179153.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011888.1 Saccharomyces cerevisiae S288C myosin 1 (MYO1), partial mRNA.

Price & Availability↑

CloneID	OSi05221
Clone ID Related Accession (Same CDS sequence)	NM_001179153.1
Accession Version	NM_001179153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	myosin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACCGGCG GGCAGTCTTG CAGTTCTAAT ATGATCGTCT GGATACCAGA TGAGAAAGAA 
GTTTTCGTAA AAGGGGAGCT GATGAGTACC GATATCAACA AGAATAAATT TACAGGCCAA 
GAAGAACAAA TAGGAATTGT CCACCCGTTG GATTCCACAG AGGTTTCTAA TCTAGTGCAG 
GTACGAATAT CCGATGTATT TCCAGTTAAC CCATCGACTT TTGACAAAGT AGAGAATATG 
TCTGAGTTAA CCCATTTGAA TGAGCCGTCT GTGCTTTATA ACCTAGAAAA GAGGTACGAT 
TGTGATCTAA TATACACCTA TTCCGGCTTA TTTTTAGTGG CGATTAACCC TTATCACAAT 
TTGAATTTAT ATTCTGAAGA CCATATAAAC TTGTACCACA ACAAGCATAA CAGGTTATCA 
AAGAGCAGAT TGGATGAAAA CTCCCATGAA AAACTGCCGC CGCATATATT TGCAATTGCA 
GAAGAAGCCT ATGAGAATCT TTTATCTGAA GGAAAAGATC AATCTATTTT AGTCACTGGT 
GAATCCGGTG CGGGCAAGAC AGAAAATACG AAAAAGATTC TACAATATCT AGCATCTATA 
ACTTCTGGCT CTCCTTCCAA TATAGCTCCT GTTAGTGGTA GTTCTATTGT AGAAAGCTTC 
GAAATGAAAA TTCTACAAAG TAACCCTATC TTAGAGTCTT TTGGTAATGC ACAGACTGTA 
CGAAATAACA ACTCTTCAAG ATTCGGTAAA TTCATAAAGA TAGAATTTAA TGAACATGGT 
ATGATCAATG GTGCGCATAT CGAGTGGTAC CTTTTAGAGA AATCAAGAAT TGTTCATCAA 
AATTCGAAAG AAAGAAATTA TCATATATTT TACCAACTAT TATCCGGTTT AGACGATTCT 
GAGTTGAAAA ATCTACGCCT TAAATCTAGG AACGTAAAAG ATTACAAAAT TTTATCCAAT 
TCTAACCAGG ATATTATACC AGGAATCAAC GACGTAGAGA ATTTTAAAGA ACTGCTCTCG 
GCATTGAATA TTATTGGGTT TTCAAAAGAT CAAATAAGAT GGATATTTCA AGTAGTGGCA 
ATTATTTTAT TAATCGGTAA CATTGAGTTC GTATCAGACA GAGCAGAACA AGCATCTTTC 
AAAAATGATG TTAGCGCAAT TTGTAGCAAT TTAGGCGTGG ACGAAAAAGA TTTCCAAACT 
GCCATATTAA GGCCTAGATC AAAAGCCGGA AAAGAGTGGG TTTCACAGTC CAAAAACTCT 
CAACAAGCTA AGTTCATCTT GAATGCCTTA TCAAGAAATC TTTATGAGCG GTTGTTCGGA 
TATATTGTGG ATATGATTAA TAAAAACTTG GACCATGGGA GTGCAACTTT GAATTACATT 
GGATTGTTGG ATATTGCTGG TTTTGAAATA TTCGAAAATA ATTCCTTTGA ACAATTATGC 
ATCAACTATA CAAATGAAAA ATTACAGCAG TTCTTTAATA ACCATATGTT TGTTTTAGAA 
CAGAGCGAAT ATTTAAAGGA AAACATTCAA TGGGATTATA TAGATTACGG AAAAGATTTG 
CAACTGACGA TTGATTTGAT CGAAAGCAAG GGGCCACCGA CCGGTGTACT ACCGTTGTTG 
GATGAGGAAG CCGTTTTGCC CAAATCCACT GATGAGTCAT TCTACTCTAA ACTGATCTCA 
ACTTGGGACC AAAACTCTTC AAAGTTTAAA CGTTCAAGAT TAAAAAATGG GTTCATTTTG 
AAACACTATG CTGGGGATGT GGAATACACT GTGGAAGGCT GGTTATCCAA AAACAAAGAT 
CCTTTAAACG ATAATCTCTT GTCTTTGTTG TCTTCTTCAC AAAACGATAT CATTTCAAAA 
CTGTTCCAGC CAGAGGGCGA AAAATCTTCT AGTGCTGGTG TGGAAGCCAA CATCTCCAAC 
CAAGAAGTTA AGAAATCAGC TAGGACAAGT ACCTTCAAGA CTACATCATC ACGCCATAGA 
GAACAGCAGA TAACATTACT AAATCAACTA GCTTCTACCC ACCCACATTT TGTTCGTTGT 
ATAATTCCAA ACAACGTGAA AAAAGTGAAA ACATTTAACA GAAGGTTAAT CTTAGATCAA 
TTACGTTGTA ATGGTGTGCT AGAGGGTATT AGACTTGCCA GAGAAGGTTA CCCAAATAGG 
ATAGCATTCC AAGAATTTTT CCAGCGGTAT AGGATCTTGT ATCCTGAAAA TTCAACCACC 
ACGACTTTCA GTTCTAAATT AAAAGCCAGT ACCAAACAAA ACTGTGAGTT TCTTCTAACG 
TCTTTGCAAC TGGATACAAA AGTTTATAAA ATTGGTAATA CTAAACTGTT TTTCAAAGCT 
GGAGTATTGG CAGATTTGGA AAAACAAAAA GATGTTAAGC TGAATAATAT TATGATTAAA 
CTAACAGCAA CTATACGAGG TTACACAGTA AGAAAAGAAA TAACGTACCA TCTACAAAAA 
TTAAAGAAAA CAAGGGTGAT TGGTAATACC TTCAGATTAT ACAATAGACT GGTGAAGGAA 
GATCCTTGGT TTAATTTATT TATCAGGATC AAGCCACTTT TAACATCATC CAATGACATG 
ACCAGAACCA AAAAATTCAA CGAGCAAATT AATAAACTGA AGAACGACCT TCAAGAAATG 
GAATCTAAGA AGAAGTTTTT GGAAGAAAAG AACCAAAAAA CTGTCAATGA GTTGGAAAAT 
ACGCAGGACT TGCTAAATCA GGAGAAAGAG AATTTAAGAA AAAATGAGAG CTTATTAAAT 
AGAGTTAAAA CCAGCTCAGA AACATTACAA AAACAATTCG ATGACCTTGT TTCTGAAAAG 
GATGAAATTA GCAGGGAAAA ACTAGAAGTT GCGCAAAATC TTGAAGAAGC TCATCAAAAA 
ATCCAAGGCC TTCAAGAAAC TATTAGAGAA CGGGAGGCGA CCTTAGAGAA ATTACACTCA 
AAAAATAACG AACTAATAAA GCAAATATCA GACTTGAACT GTGACATTTC TAAGGAACAG 
AGCTCCCAGT CTTTAATCAA AGAATCTAAA TTGAAGCTAG AAAACGAAAT AAAGAGATTA 
AAAGATGTCA TAAACTCGAA GGAGGAAGAA ATAAAATCGT TCAACGATAA GTTAAGTTCC 
TCAGAAGAAG ATCTTGACAT AAAATTGGTC ACTTTAGAGA AAAATTGTAA CATTGCGATG 
TCAAGACTAC AATCCCTTGT AACAGAAAAT TCAGATTTGC GTTCGAAAAA TGAGAATTTC 
AAGAAAGAAA AGGCAGCACT GAATAACCAG TTGAAAAATA AGGAAAGTGA ACTGTTAAAG 
ATGAAAGAAA AAATAGATAA TCACAAAAAA GAGCTTGCTA CTTTTTCCAA GCAAAGAGAT 
GACGCAGTTA GCGAACATGG TAAAATTACT GCTGAACTAA AAGAGACAAG AATCCAACTT 
ACCGAGTATA AATCTAACTA TCAAAAGATT AAAGAAGAAT ATTCCAACTT CCAAAGAGAA 
ACGAAGGAAC AGGAGCAAAA GAAAAGAAAT AGCCTGGTTG AGTCTCTGAA CGATAGTAAA 
ATTAAAGAAT TGGAAGCTCG GTTGTCACAG GAAATATCCT TAAATCAATA CTTAAACAAA 
AGAATATCAG GAAATTCTGT AGAAACGAAT ATTTCGTCTA CTAGACGTTC CACATCGTAT 
TCAGATGATC CACTTGACAA AGAAGATATA ATAAAAAAAT ACTATGACCT TCAATTAGCG 
TTTACTGAAA TAACTAGGAA TCTAGAGAAT GAAATTGAAG AGAAGAAGAA CTTAATTTCT 
AGATTGAGAT TCACTGAAAC AAGACTAGCA TCTTCGTCTT TTGAGGACCA AAAGATTAAG 
GCACAAATGA AGAAATTAAA AAAATTGATC CAGGATATGG ACCCTAGTAT TCCTTTGGAC 
AGTATTCTAA ATGAGCCGCT AGATAACTGC CCTGACAAAG AGTCTGATAT TAACAAATTA 
ATGCTTGAGG TCGATTATTT AAAAAGACAA TTGGATATCG AAACAAGAGC TCACTACGAT 
GCAGAAAATG CCATATCTGC TTTACACAGT AAATTTAGAA AGATCCAAGG GGAAAGCTCC 
CTGTCATCTT CTGATATTTA CAAACTGAAG TTCGAAGCCA GTGAAGAAAG AGTCAAATCC 
TTGGAAGACA AGCTAAAAAC CATGCCTTTA CGTGATCGAA CAAATTTACC TGTCGGAGAT 
ATTATAAAGA ACCGTGATAG CATTTCAAAA TATGAAGAAG AAATTCGCTA TTATAAACTT 
GAAAACTACA AGCTCCAAGA AATATTAAAT GAATCAAATG GAAAATTGAG CCAACTCACT 
CTTGACTTGA GGCAATCAAA ATCCAAAGAA GCCCTACTTA GTGAACAATT AGACAGATTA 
CAGAAAGACC TTGAATCTAC GGAACGCCAA AAAGAACTAC TATCATCCAC AATAAAACAG 
CAGAAACAGC AATTTGAAAA CTGCATGGAC GATCTGCAAG GGAATGAACT GCGGCTAAGA 
GAACATATTC ATGCACTAAA ACAAGCTGAA GAGGACGTAA AGAATATGGC GTCTATCATT 
GAAAAATTGA AAACTCAAAA TAAGCAAAAG GAAAAGTTAA TCTGGGAGCG CGAAATGGAA 
AGAAATGACT CAGATATGCA ATTACAGGAA ACTCTGCTGG AGTTGAAGAG AGTCCAAGAT 
GTAAAGAAAA TCCTGAGTGA TGATTTGGCC CATTTAAAGG AGCGATTGAG CGCTGTAGAA 
GATAGATCCC AATATACGGA TGAGATTAAT AGATTAAAGG AAGAATTGAA CTGCTCTTTA 
AAAGCTGAAA CAAACTTAAA AAAAGAATTT GCAACCCTTA AGTACAAACT GGAAACTTCG 
ACTAATGATT CTGAAGCAAA AATCTCCGAT CTGCTGAAGC AGCTCGATCA TTATACAAAA 
GTGGTGGAAA TGTTGAACAA CGAAAAAGAT GCTATTTCTC TTGCAGAAAA AGAACTTTAT 
CAGAAATACG AGGCACTCAA TACTGAATGC GAGTCTCTTA AAGGAAAGAT AGTGTCTTTG 
ACTAAAATCA AGCAGGAACT GGAATCTGAC TTAAATCAAA AGACTGATGC GCTACAGATA 
TCAAACGCAG CACTCTCAAG TTCCACACAA AAGAATAAAG AGATTACTGA AAAAATCAAA 
TATCTGGAGG AAACACTACA ACTGCAAATG GAACAAAATT CAAGGAATGG GGAGTTGGTT 
AAGACATTAC AGGCCAGCTG CAATGGATAT AAAGATAAGT TTGATGATGA GAAGCAGAAG 
AATATTGATT TATATGAAGA AAATCAAACT TTACAAAAGC TCAACACCGA CTTACAGCTT 
CAACTAAAAA ATTTGCATGA AAGATTATCA GACACTACTG AAAAAAACGC ATGGTTATCA 
AAGATTCATG AATTGGAAAA TATGGTAAGC CTAGAAACGG ACTTGAAATA TGAAGAAATG 
AAAAAGAACA AGAGTTTGGA GAGAGCAGTG GAAGAGTTAC AAACTAAGAA CTCCCAACAA 
ACAGATGTAA TAGAACTAGC GAATAAAAAT AGAAGCGAAT TTGAGGAAGC TACTTTGAAA 
TATGAGGCTC AAATCTCTGA CTTAGAAAAG TACATTTCTC AACAAGAGCT TGAGATGAAA 
AAATCCATTA GAGATAATTC TTCTTACCGC GACAAAGTGC AGGAAATGGC CCAAGAAATT 
GAATTCTGGA AAAGCCGTTA TGAATCTACC ATGATAGGCT CGAAAAATAT TGATAGTAAC 
AATGCACAGA GTAAAATTTT CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011888.1
CDS	1..5787
Translation	MTGGQSCSSNMIVWIPDEKEVFVKGELMSTDINKNKFTGQEEQIGIVHPLDSTEVSNLVQVRISDVFPVNPSTFDKVENMSELTHLNEPSVLYNLEKRYDCDLIYTYSGLFLVAINPYHNLNLYSEDHINLYHNKHNRLSKSRLDENSHEKLPPHIFAIAEEAYENLLSEGKDQSILVTGESGAGKTENTKKILQYLASITSGSPSNIAPVSGSSIVESFEMKILQSNPILESFGNAQTVRNNNSSRFGKFIKIEFNEHGMINGAHIEWYLLEKSRIVHQNSKERNYHIFYQLLSGLDDSELKNLRLKSRNVKDYKILSNSNQDIIPGINDVENFKELLSALNIIGFSKDQIRWIFQVVAIILLIGNIEFVSDRAEQASFKNDVSAICSNLGVDEKDFQTAILRPRSKAGKEWVSQSKNSQQAKFILNALSRNLYERLFGYIVDMINKNLDHGSATLNYIGLLDIAGFEIFENNSFEQLCINYTNEKLQQFFNNHMFVLEQSEYLKENIQWDYIDYGKDLQLTIDLIESKGPPTGVLPLLDEEAVLPKSTDESFYSKLISTWDQNSSKFKRSRLKNGFILKHYAGDVEYTVEGWLSKNKDPLNDNLLSLLSSSQNDIISKLFQPEGEKSSSAGVEANISNQEVKKSARTSTFKTTSSRHREQQITLLNQLASTHPHFVRCIIPNNVKKVKTFNRRLILDQLRCNGVLEGIRLAREGYPNRIAFQEFFQRYRILYPENSTTTTFSSKLKASTKQNCEFLLTSLQLDTKVYKIGNTKLFFKAGVLADLEKQKDVKLNNIMIKLTATIRGYTVRKEITYHLQKLKKTRVIGNTFRLYNRLVKEDPWFNLFIRIKPLLTSSNDMTRTKKFNEQINKLKNDLQEMESKKKFLEEKNQKTVNELENTQDLLNQEKENLRKNESLLNRVKTSSETLQKQFDDLVSEKDEISREKLEVAQNLEEAHQKIQGLQETIREREATLEKLHSKNNELIKQISDLNCDISKEQSSQSLIKESKLKLENEIKRLKDVINSKEEEIKSFNDKLSSSEEDLDIKLVTLEKNCNIAMSRLQSLVTENSDLRSKNENFKKEKAALNNQLKNKESELLKMKEKIDNHKKELATFSKQRDDAVSEHGKITAELKETRIQLTEYKSNYQKIKEEYSNFQRETKEQEQKKRNSLVESLNDSKIKELEARLSQEISLNQYLNKRISGNSVETNISSTRRSTSYSDDPLDKEDIIKKYYDLQLAFTEITRNLENEIEEKKNLISRLRFTETRLASSSFEDQKIKAQMKKLKKLIQDMDPSIPLDSILNEPLDNCPDKESDINKLMLEVDYLKRQLDIETRAHYDAENAISALHSKFRKIQGESSLSSSDIYKLKFEASEERVKSLEDKLKTMPLRDRTNLPVGDIIKNRDSISKYEEEIRYYKLENYKLQEILNESNGKLSQLTLDLRQSKSKEALLSEQLDRLQKDLESTERQKELLSSTIKQQKQQFENCMDDLQGNELRLREHIHALKQAEEDVKNMASIIEKLKTQNKQKEKLIWEREMERNDSDMQLQETLLELKRVQDVKKILSDDLAHLKERLSAVEDRSQYTDEINRLKEELNCSLKAETNLKKEFATLKYKLETSTNDSEAKISDLLKQLDHYTKVVEMLNNEKDAISLAEKELYQKYEALNTECESLKGKIVSLTKIKQELESDLNQKTDALQISNAALSSSTQKNKEITEKIKYLEETLQLQMEQNSRNGELVKTLQASCNGYKDKFDDEKQKNIDLYEENQTLQKLNTDLQLQLKNLHERLSDTTEKNAWLSKIHELENMVSLETDLKYEEMKKNKSLERAVEELQTKNSQQTDVIELANKNRSEFEEATLKYEAQISDLEKYISQQELEMKKSIRDNSSYRDKVQEMAQEIEFWKSRYESTMIGSKNIDSNNAQSKIFS

Target ORF information:

RefSeq Version	NM_001179153.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C myosin 1 (MYO1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179153.1

ORF Insert Sequence:

ATGACCGGCG GGCAGTCTTG CAGTTCTAAT ATGATCGTCT GGATACCAGA TGAGAAAGAA 
GTTTTCGTAA AAGGGGAGCT GATGAGTACC GATATCAACA AGAATAAATT TACAGGCCAA 
GAAGAACAAA TAGGAATTGT CCACCCGTTG GATTCCACAG AGGTTTCTAA TCTAGTGCAG 
GTACGAATAT CCGATGTATT TCCAGTTAAC CCATCGACTT TTGACAAAGT AGAGAATATG 
TCTGAGTTAA CCCATTTGAA TGAGCCGTCT GTGCTTTATA ACCTAGAAAA GAGGTACGAT 
TGTGATCTAA TATACACCTA TTCCGGCTTA TTTTTAGTGG CGATTAACCC TTATCACAAT 
TTGAATTTAT ATTCTGAAGA CCATATAAAC TTGTACCACA ACAAGCATAA CAGGTTATCA 
AAGAGCAGAT TGGATGAAAA CTCCCATGAA AAACTGCCGC CGCATATATT TGCAATTGCA 
GAAGAAGCCT ATGAGAATCT TTTATCTGAA GGAAAAGATC AATCTATTTT AGTCACTGGT 
GAATCCGGTG CGGGCAAGAC AGAAAATACG AAAAAGATTC TACAATATCT AGCATCTATA 
ACTTCTGGCT CTCCTTCCAA TATAGCTCCT GTTAGTGGTA GTTCTATTGT AGAAAGCTTC 
GAAATGAAAA TTCTACAAAG TAACCCTATC TTAGAGTCTT TTGGTAATGC ACAGACTGTA 
CGAAATAACA ACTCTTCAAG ATTCGGTAAA TTCATAAAGA TAGAATTTAA TGAACATGGT 
ATGATCAATG GTGCGCATAT CGAGTGGTAC CTTTTAGAGA AATCAAGAAT TGTTCATCAA 
AATTCGAAAG AAAGAAATTA TCATATATTT TACCAACTAT TATCCGGTTT AGACGATTCT 
GAGTTGAAAA ATCTACGCCT TAAATCTAGG AACGTAAAAG ATTACAAAAT TTTATCCAAT 
TCTAACCAGG ATATTATACC AGGAATCAAC GACGTAGAGA ATTTTAAAGA ACTGCTCTCG 
GCATTGAATA TTATTGGGTT TTCAAAAGAT CAAATAAGAT GGATATTTCA AGTAGTGGCA 
ATTATTTTAT TAATCGGTAA CATTGAGTTC GTATCAGACA GAGCAGAACA AGCATCTTTC 
AAAAATGATG TTAGCGCAAT TTGTAGCAAT TTAGGCGTGG ACGAAAAAGA TTTCCAAACT 
GCCATATTAA GGCCTAGATC AAAAGCCGGA AAAGAGTGGG TTTCACAGTC CAAAAACTCT 
CAACAAGCTA AGTTCATCTT GAATGCCTTA TCAAGAAATC TTTATGAGCG GTTGTTCGGA 
TATATTGTGG ATATGATTAA TAAAAACTTG GACCATGGGA GTGCAACTTT GAATTACATT 
GGATTGTTGG ATATTGCTGG TTTTGAAATA TTCGAAAATA ATTCCTTTGA ACAATTATGC 
ATCAACTATA CAAATGAAAA ATTACAGCAG TTCTTTAATA ACCATATGTT TGTTTTAGAA 
CAGAGCGAAT ATTTAAAGGA AAACATTCAA TGGGATTATA TAGATTACGG AAAAGATTTG 
CAACTGACGA TTGATTTGAT CGAAAGCAAG GGGCCACCGA CCGGTGTACT ACCGTTGTTG 
GATGAGGAAG CCGTTTTGCC CAAATCCACT GATGAGTCAT TCTACTCTAA ACTGATCTCA 
ACTTGGGACC AAAACTCTTC AAAGTTTAAA CGTTCAAGAT TAAAAAATGG GTTCATTTTG 
AAACACTATG CTGGGGATGT GGAATACACT GTGGAAGGCT GGTTATCCAA AAACAAAGAT 
CCTTTAAACG ATAATCTCTT GTCTTTGTTG TCTTCTTCAC AAAACGATAT CATTTCAAAA 
CTGTTCCAGC CAGAGGGCGA AAAATCTTCT AGTGCTGGTG TGGAAGCCAA CATCTCCAAC 
CAAGAAGTTA AGAAATCAGC TAGGACAAGT ACCTTCAAGA CTACATCATC ACGCCATAGA 
GAACAGCAGA TAACATTACT AAATCAACTA GCTTCTACCC ACCCACATTT TGTTCGTTGT 
ATAATTCCAA ACAACGTGAA AAAAGTGAAA ACATTTAACA GAAGGTTAAT CTTAGATCAA 
TTACGTTGTA ATGGTGTGCT AGAGGGTATT AGACTTGCCA GAGAAGGTTA CCCAAATAGG 
ATAGCATTCC AAGAATTTTT CCAGCGGTAT AGGATCTTGT ATCCTGAAAA TTCAACCACC 
ACGACTTTCA GTTCTAAATT AAAAGCCAGT ACCAAACAAA ACTGTGAGTT TCTTCTAACG 
TCTTTGCAAC TGGATACAAA AGTTTATAAA ATTGGTAATA CTAAACTGTT TTTCAAAGCT 
GGAGTATTGG CAGATTTGGA AAAACAAAAA GATGTTAAGC TGAATAATAT TATGATTAAA 
CTAACAGCAA CTATACGAGG TTACACAGTA AGAAAAGAAA TAACGTACCA TCTACAAAAA 
TTAAAGAAAA CAAGGGTGAT TGGTAATACC TTCAGATTAT ACAATAGACT GGTGAAGGAA 
GATCCTTGGT TTAATTTATT TATCAGGATC AAGCCACTTT TAACATCATC CAATGACATG 
ACCAGAACCA AAAAATTCAA CGAGCAAATT AATAAACTGA AGAACGACCT TCAAGAAATG 
GAATCTAAGA AGAAGTTTTT GGAAGAAAAG AACCAAAAAA CTGTCAATGA GTTGGAAAAT 
ACGCAGGACT TGCTAAATCA GGAGAAAGAG AATTTAAGAA AAAATGAGAG CTTATTAAAT 
AGAGTTAAAA CCAGCTCAGA AACATTACAA AAACAATTCG ATGACCTTGT TTCTGAAAAG 
GATGAAATTA GCAGGGAAAA ACTAGAAGTT GCGCAAAATC TTGAAGAAGC TCATCAAAAA 
ATCCAAGGCC TTCAAGAAAC TATTAGAGAA CGGGAGGCGA CCTTAGAGAA ATTACACTCA 
AAAAATAACG AACTAATAAA GCAAATATCA GACTTGAACT GTGACATTTC TAAGGAACAG 
AGCTCCCAGT CTTTAATCAA AGAATCTAAA TTGAAGCTAG AAAACGAAAT AAAGAGATTA 
AAAGATGTCA TAAACTCGAA GGAGGAAGAA ATAAAATCGT TCAACGATAA GTTAAGTTCC 
TCAGAAGAAG ATCTTGACAT AAAATTGGTC ACTTTAGAGA AAAATTGTAA CATTGCGATG 
TCAAGACTAC AATCCCTTGT AACAGAAAAT TCAGATTTGC GTTCGAAAAA TGAGAATTTC 
AAGAAAGAAA AGGCAGCACT GAATAACCAG TTGAAAAATA AGGAAAGTGA ACTGTTAAAG 
ATGAAAGAAA AAATAGATAA TCACAAAAAA GAGCTTGCTA CTTTTTCCAA GCAAAGAGAT 
GACGCAGTTA GCGAACATGG TAAAATTACT GCTGAACTAA AAGAGACAAG AATCCAACTT 
ACCGAGTATA AATCTAACTA TCAAAAGATT AAAGAAGAAT ATTCCAACTT CCAAAGAGAA 
ACGAAGGAAC AGGAGCAAAA GAAAAGAAAT AGCCTGGTTG AGTCTCTGAA CGATAGTAAA 
ATTAAAGAAT TGGAAGCTCG GTTGTCACAG GAAATATCCT TAAATCAATA CTTAAACAAA 
AGAATATCAG GAAATTCTGT AGAAACGAAT ATTTCGTCTA CTAGACGTTC CACATCGTAT 
TCAGATGATC CACTTGACAA AGAAGATATA ATAAAAAAAT ACTATGACCT TCAATTAGCG 
TTTACTGAAA TAACTAGGAA TCTAGAGAAT GAAATTGAAG AGAAGAAGAA CTTAATTTCT 
AGATTGAGAT TCACTGAAAC AAGACTAGCA TCTTCGTCTT TTGAGGACCA AAAGATTAAG 
GCACAAATGA AGAAATTAAA AAAATTGATC CAGGATATGG ACCCTAGTAT TCCTTTGGAC 
AGTATTCTAA ATGAGCCGCT AGATAACTGC CCTGACAAAG AGTCTGATAT TAACAAATTA 
ATGCTTGAGG TCGATTATTT AAAAAGACAA TTGGATATCG AAACAAGAGC TCACTACGAT 
GCAGAAAATG CCATATCTGC TTTACACAGT AAATTTAGAA AGATCCAAGG GGAAAGCTCC 
CTGTCATCTT CTGATATTTA CAAACTGAAG TTCGAAGCCA GTGAAGAAAG AGTCAAATCC 
TTGGAAGACA AGCTAAAAAC CATGCCTTTA CGTGATCGAA CAAATTTACC TGTCGGAGAT 
ATTATAAAGA ACCGTGATAG CATTTCAAAA TATGAAGAAG AAATTCGCTA TTATAAACTT 
GAAAACTACA AGCTCCAAGA AATATTAAAT GAATCAAATG GAAAATTGAG CCAACTCACT 
CTTGACTTGA GGCAATCAAA ATCCAAAGAA GCCCTACTTA GTGAACAATT AGACAGATTA 
CAGAAAGACC TTGAATCTAC GGAACGCCAA AAAGAACTAC TATCATCCAC AATAAAACAG 
CAGAAACAGC AATTTGAAAA CTGCATGGAC GATCTGCAAG GGAATGAACT GCGGCTAAGA 
GAACATATTC ATGCACTAAA ACAAGCTGAA GAGGACGTAA AGAATATGGC GTCTATCATT 
GAAAAATTGA AAACTCAAAA TAAGCAAAAG GAAAAGTTAA TCTGGGAGCG CGAAATGGAA 
AGAAATGACT CAGATATGCA ATTACAGGAA ACTCTGCTGG AGTTGAAGAG AGTCCAAGAT 
GTAAAGAAAA TCCTGAGTGA TGATTTGGCC CATTTAAAGG AGCGATTGAG CGCTGTAGAA 
GATAGATCCC AATATACGGA TGAGATTAAT AGATTAAAGG AAGAATTGAA CTGCTCTTTA 
AAAGCTGAAA CAAACTTAAA AAAAGAATTT GCAACCCTTA AGTACAAACT GGAAACTTCG 
ACTAATGATT CTGAAGCAAA AATCTCCGAT CTGCTGAAGC AGCTCGATCA TTATACAAAA 
GTGGTGGAAA TGTTGAACAA CGAAAAAGAT GCTATTTCTC TTGCAGAAAA AGAACTTTAT 
CAGAAATACG AGGCACTCAA TACTGAATGC GAGTCTCTTA AAGGAAAGAT AGTGTCTTTG 
ACTAAAATCA AGCAGGAACT GGAATCTGAC TTAAATCAAA AGACTGATGC GCTACAGATA 
TCAAACGCAG CACTCTCAAG TTCCACACAA AAGAATAAAG AGATTACTGA AAAAATCAAA 
TATCTGGAGG AAACACTACA ACTGCAAATG GAACAAAATT CAAGGAATGG GGAGTTGGTT 
AAGACATTAC AGGCCAGCTG CAATGGATAT AAAGATAAGT TTGATGATGA GAAGCAGAAG 
AATATTGATT TATATGAAGA AAATCAAACT TTACAAAAGC TCAACACCGA CTTACAGCTT 
CAACTAAAAA ATTTGCATGA AAGATTATCA GACACTACTG AAAAAAACGC ATGGTTATCA 
AAGATTCATG AATTGGAAAA TATGGTAAGC CTAGAAACGG ACTTGAAATA TGAAGAAATG 
AAAAAGAACA AGAGTTTGGA GAGAGCAGTG GAAGAGTTAC AAACTAAGAA CTCCCAACAA 
ACAGATGTAA TAGAACTAGC GAATAAAAAT AGAAGCGAAT TTGAGGAAGC TACTTTGAAA 
TATGAGGCTC AAATCTCTGA CTTAGAAAAG TACATTTCTC AACAAGAGCT TGAGATGAAA 
AAATCCATTA GAGATAATTC TTCTTACCGC GACAAAGTGC AGGAAATGGC CCAAGAAATT 
GAATTCTGGA AAAGCCGTTA TGAATCTACC ATGATAGGCT CGAAAAATAT TGATAGTAAC 
AATGCACAGA GTAAAATTTT CAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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MYO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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