Myh7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh7
Entrez Gene ID	29557
Full Name	myosin heavy chain 7
Synonyms	Bmyo,Myhcb,myHC-beta,myHC-slow
General protein information	Preferred Names myosin heavy chain 7 Names myosin-7 beta myosin heavy chain myosin heavy chain polypeptide 7 cardiac muscle fetal myosin heavy chain slow isoform myosin heavy chain, cardiac muscle beta isoform myosin heavy chain, cardiac muscle, fetal myosin heavy chain, polypeptide 7 myosin, heavy chain 7, cardiac muscle, beta myosin, heavy polypeptide 7, cardiac muscle, beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017240.2	NP_058936.1	myosin-7
XM_006251951.2	XP_006252013.1	myosin-7 isoform X2
XM_017599642.1	XP_017455131.1	myosin-7 isoform X1
XM_017599643.1	XP_017455132.1	myosin-7 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	smyhc1	NP_001018343.1
Danio rerio (zebrafish)	vmhcl	NP_001070932.2
Drosophila melanogaster (fruit fly)	Mhc	NP_001246049.1
Rattus norvegicus (Norway rat)	Myh7	NP_058936.1
Danio rerio (zebrafish)	LOC100329748	XP_002666778.1
Eremothecium gossypii	AGOS_ACR068W	NP_983470.2
Homo sapiens (human)	MYH7	NP_000248.2
Canis lupus familiaris (dog)	MYH7	NP_001107183.1
Mus musculus (house mouse)	Myh7	NP_542766.1
Danio rerio (zebrafish)	vmhc	NP_001106204.1
Danio rerio (zebrafish)	si:ch211-24n20.3	XP_001923213.1
Danio rerio (zebrafish)	smyhc2	NP_001096096.2
Danio rerio (zebrafish)	LOC100329813	XP_002666779.1
Saccharomyces cerevisiae (baker's yeast)	MYO1	NP_011888.1
Kluyveromyces lactis	KLLA0E11595g	XP_454473.1
Pan troglodytes (chimpanzee)	MYH7	XP_001150786.3
Bos taurus (cattle)	MYH7	NP_777152.1
Danio rerio (zebrafish)	LOC100329670	XP_002666777.2
Danio rerio (zebrafish)	LOC567740	XP_696132.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010147	XP_319309.3
Xenopus tropicalis (tropical clawed frog)	myh7	XP_002939061.1
Gallus gallus (chicken)	MYH7	NP_001001302.1
Danio rerio (zebrafish)	smyhc3	NP_001129995.1
Schizosaccharomyces pombe (fission yeast)	myo2	NP_588114.1

Gene Ontology
Bibliography

Related articles in PubMed

Impact of MYH6 variants in hypoplastic left heart syndrome.
Tomita-Mitchell A, Stamm KD, Mahnke DK, Kim MS, Hidestrand PM, Liang HL, Goetsch MA, Hidestrand M, Simpson P, Pelech AN, Tweddell JS, Benson DW, Lough JW, Mitchell ME
Physiological genomics48(12)912-921(2016 Dec)

Rat cardiac troponin T mutation (F72L)-mediated impact on thin filament cooperativity is divergently modulated by ?- and ?-myosin heavy chain isoforms.
Chandra V, Gollapudi SK, Chandra M
American journal of physiology. Heart and circulatory physiology309(8)H1260-70(2015 Oct)

l-Arginine Attenuates Cardiac Dysfunction, But Further Down-Regulates ?-Myosin Heavy Chain Expression in Isoproterenol-Induced Cardiomyopathy.
Kralova E, Doka G, Pivackova L, Srankova J, Kuracinova K, Janega P, Babal P, Klimas J, Krenek P
Basic & clinical pharmacology & toxicology117(4)251-60(2015 Oct)

Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly.
Taylor KC, Buvoli M, Korkmaz EN, Buvoli A, Zheng Y, Heinze NT, Cui Q, Leinwand LA, Rayment I
Proceedings of the National Academy of Sciences of the United States of America112(29)E3806-15(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TnT mutation F72L leads to contractile changes that are linked to dilated cardiomyopathy in the presence of MYH6 and hypertrophic cardiomyopathy in the presence of MYH7.
Title: Rat cardiac troponin T mutation (F72L)-mediated impact on thin filament cooperativity is divergently modulated by ?- and ?-myosin heavy chain isoforms.

Myocardial stiffness in a rat model of diabetes is studied. Short-range force, a cross-bridge based property, is increased in diabetic hearts. An increase in beta myosin heavy chain isoform expression is the likely mechanism.
Title: Increased myocardial short-range forces in a rodent model of diabetes reflect elevated content of ? myosin heavy chain.

results indicate that T3-bound TRs interfere with transactivation by TEADs via protein-protein interactions, resulting in the negative regulation of MYH7 promoter activity
Title: Essential role of TEA domain transcription factors in the negative regulation of the MYH 7 gene by thyroid hormone and its receptors.

Cardiomyopathy mutations in the tail of beta-cardiac myosin modify the coiled-coil structure and affect integration into thick filaments in muscle sarcomeres in adult cardiomyocytes.
Title: Cardiomyopathy mutations in the tail of ?-cardiac myosin modify the coiled-coil structure and affect integration into thick filaments in muscle sarcomeres in adult cardiomyocytes.

visual stimulation may play a role in the signals that regulate both nmMyH IIB and EO-MyHC developmental transitions
Title: Postnatal changes in the developing rat extraocular muscles.

The following Myh7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58566	XM_017599643.1 Latest version!	Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa03353	NM_017240.2 Latest version!	Rattus norvegicus myosin heavy chain 7 (Myh7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa36660	XM_006251951.2 Latest version!	Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa58565	XM_017599642.1 Latest version!	Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh7 ( XM_017599643.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455132.1 Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa58566
Clone ID Related Accession (Same CDS sequence)	XM_017599643.1
Accession Version	XM_017599643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG CAGAGATGGC TGCATTTGGG GCCGCAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GCGCTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGGA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACTGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGAG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGGGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCATCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCTCATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGAGCAG 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCAGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA TGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TCAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGGTG 
AGGGGGTTAG GGACCCTGCG CAGAAACCCA CTGAGAGTCC GGGCCCAGGA TAATTCTAGA 
GCAAGTCAAG CAGCATTGCA AAGCATCTCA CTGCTTGGGT CAGCTCTCAT CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455132.1
CDS	159..5435
Translation	MADAEMAAFGAAAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPVDKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQVRGLGTLRRNPLRVRAQDNSRASQAALQSISLLGSALIC

Target ORF information:

RefSeq Version	XM_017599643.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599643.1

ORF Insert Sequence:

ATGGCGGATG CAGAGATGGC TGCATTTGGG GCCGCAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GCGCTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGGA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACTGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGAG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGGGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCATCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCTCATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGAGCAG 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCAGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA TGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TCAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGGTG 
AGGGGGTTAG GGACCCTGCG CAGAAACCCA CTGAGAGTCC GGGCCCAGGA TAATTCTAGA 
GCAAGTCAAG CAGCATTGCA AAGCATCTCA CTGCTTGGGT CAGCTCTCAT CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7 ( NM_017240.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058936.1 Rattus norvegicus myosin heavy chain 7 (Myh7), mRNA.

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CloneID	ORa03353
Clone ID Related Accession (Same CDS sequence)	NM_017240.2
Accession Version	NM_017240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X15939.1. On Feb 21, 2013 this sequence version replaced NM_017240.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X15939.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATC GAGAGATGGC TGCATTTGGG GCCGGAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GAGGTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGCA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACCGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCTTCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCACATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGATCAG 
GACAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCCGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA CGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG TTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TGAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCAGACC TCTCCCAGCT CCAGACAGAG 
GTGGAGGAGG CGGTGCAGGA GTGTAGGAAC GCAGAGGAGA AGGCCAAGAA GGCCATCACA 
GATGCCGCCA TGATGGCCGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA ATAACATGGA GCAGACCATC AAGGACCTGC AGCACCGGCT GGACGAGGCA 
GAGCAGATCG CCCTCAAGGG TGGCAAGAAG CAGCTGCAGA AGCTGGAGGC CCGGGTCCGG 
GAGCTGGAGA ATGAGCTGGA GGCTGAGCAG AAGCGCAATG CGGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CAGAGGAAGA CAGGAAGAAC 
CTACTGCGAC TGCAGGACCT GGTGGACAAG CTGCAGTTAA AGGTGAAGGC CTACAAGCGC 
CAGGCTGAGG AGGCGGAGGA ACAGGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGAGGGCG GACATTGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCGCCAAG GGCCTGAATG AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058936.1
CDS	9..5816
Translation	MADREMAAFGAGAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAQVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFFSQGETTVASIDDSEEHMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPVDKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLDQDKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTRQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILVEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKNNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKGLNEE

Target ORF information:

RefSeq Version	NM_017240.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 7 (Myh7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017240.2

ORF Insert Sequence:

ATGGCGGATC GAGAGATGGC TGCATTTGGG GCCGGAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GAGGTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGCA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACCGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCTTCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCACATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGATCAG 
GACAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCCGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA CGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG TTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TGAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCAGACC TCTCCCAGCT CCAGACAGAG 
GTGGAGGAGG CGGTGCAGGA GTGTAGGAAC GCAGAGGAGA AGGCCAAGAA GGCCATCACA 
GATGCCGCCA TGATGGCCGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA ATAACATGGA GCAGACCATC AAGGACCTGC AGCACCGGCT GGACGAGGCA 
GAGCAGATCG CCCTCAAGGG TGGCAAGAAG CAGCTGCAGA AGCTGGAGGC CCGGGTCCGG 
GAGCTGGAGA ATGAGCTGGA GGCTGAGCAG AAGCGCAATG CGGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CAGAGGAAGA CAGGAAGAAC 
CTACTGCGAC TGCAGGACCT GGTGGACAAG CTGCAGTTAA AGGTGAAGGC CTACAAGCGC 
CAGGCTGAGG AGGCGGAGGA ACAGGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGAGGGCG GACATTGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCGCCAAG GGCCTGAATG AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7 ( XM_006251951.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252013.1 Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X2, mRNA.

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CloneID	ORa36660
Clone ID Related Accession (Same CDS sequence)	XM_006251951.2
Accession Version	XM_006251951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006251951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG CAGAGATGGC TGCATTTGGG GCCGCAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GCGCTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGGA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACTGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGAG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGGGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCATCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCTCATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGAGCAG 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCAGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA TGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TCAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GACGCAGACC TCTCCCAGCT CCAGACAGAG 
GTGGAGGAGG CGGTGCAGGA GTGTAGGAAC GCAGAGGAGA AGGCCAAGAA GGCCATCACA 
GATGCCGCCA TGATGGCCGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA GCAGACCATC AAGGACCTGC AGCACCGGCT GGACGAGGCA 
GAGCAGATCG CCCTCAAGGG TGGCAAGAAG CAGCTGCAGA AGCTGGAGGC CCGGGTCCGG 
GAGCTGGAGA ATGAGCTGGA GGCTGAGCAG AAGCGCAATG CGGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CAGAGGAAGA CAGGAAGAAC 
CTACTGCGAC TGCAGGACCT GGTGGACAAG CTGCAGTTAA AGGTGAAGGC CTACAAGCGC 
CAGGCTGAGG AGGCGGAGGA ACAGGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGAGGGCG GACATTGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCGCCAAG GGCCTGAATG AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252013.1
CDS	159..5966
Translation	MADAEMAAFGAAAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPVDKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKGLNEE

Target ORF information:

RefSeq Version	XM_006251951.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251951.2

ORF Insert Sequence:

ATGGCGGATG CAGAGATGGC TGCATTTGGG GCCGCAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GCGCTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGGA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACTGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGAG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGGGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCATCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCTCATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGAGCAG 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCAGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA TGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TCAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GACGCAGACC TCTCCCAGCT CCAGACAGAG 
GTGGAGGAGG CGGTGCAGGA GTGTAGGAAC GCAGAGGAGA AGGCCAAGAA GGCCATCACA 
GATGCCGCCA TGATGGCCGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA GCAGACCATC AAGGACCTGC AGCACCGGCT GGACGAGGCA 
GAGCAGATCG CCCTCAAGGG TGGCAAGAAG CAGCTGCAGA AGCTGGAGGC CCGGGTCCGG 
GAGCTGGAGA ATGAGCTGGA GGCTGAGCAG AAGCGCAATG CGGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CAGAGGAAGA CAGGAAGAAC 
CTACTGCGAC TGCAGGACCT GGTGGACAAG CTGCAGTTAA AGGTGAAGGC CTACAAGCGC 
CAGGCTGAGG AGGCGGAGGA ACAGGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGAGGGCG GACATTGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCGCCAAG GGCCTGAATG AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7 ( XM_017599642.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455131.1 Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X1, mRNA.

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CloneID	ORa58565
Clone ID Related Accession (Same CDS sequence)	XM_017599642.1
Accession Version	XM_017599642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG CAGAGATGGC TGCATTTGGG GCCGCAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GCGCTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGGA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACTGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGAG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGGGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCATCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCTCATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGAGCAG 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCAGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA TGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TCAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GACGCAGACC TCTCCCAGCT CCAGACAGAG 
GTGGAGGAGG CGGTGCAGGA GTGTAGGAAC GCAGAGGAGA AGGCCAAGAA GGCCATCACA 
GATGCCGCCA TGATGGCCGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA GCAGACCATC AAGGACCTGC AGCACCGGCT GGACGAGGCA 
GAGCAGATCG CCCTCAAGGG TGGCAAGAAG CAGCTGCAGA AGCTGGAGGC CCGGGTCCGG 
GAGCTGGAGA ATGAGCTGGA GGCTGAGCAG AAGCGCAATG CGGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CAGAGGAAGA CAGGAAGAAC 
CTACTGCGAC TGCAGGACCT GGTGGACAAG CTGCAGTTAA AGGTGAAGGC CTACAAGCGC 
CAGGCTGAGG AGGCGGAGGA ACAGGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGAGGGCG GACATTGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCGCCAAG GTGGGTTCCT GCCCTCGAAT TACCAGTCAT 
GCTCTCACCA GGATACACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455131.1
CDS	159..6002
Translation	MADAEMAAFGAAAPFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPVDKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKVGSCPRITSHALTRIHL

Target ORF information:

RefSeq Version	XM_017599642.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin, heavy chain 7, cardiac muscle, beta (Myh7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599642.1

ORF Insert Sequence:

ATGGCGGATG CAGAGATGGC TGCATTTGGG GCCGCAGCCC CCTTCCTGCG AAAATCTGAG 
AAGGAGCGGC TGGAGGCGCA GACCAGGCCC TTTGACCTCA AGAAAGATGT TTTTGTGCCT 
GATGACAAAG AAGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACC 
GCTGAGACAG AGAATGGCAA GACGGTGACT GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCTCCCAAGT TCGACAAGAT CGAGGACATG GCCATGCTGA CCTTCCTGCA CGAGCCGGCT 
GTGCTCTACA ATCTCAAGGA GCGCTACGCT TCCTGGATGA TCTACACCTA CTCAGGCCTC 
TTCTGTGTCA CCGTCAACCC CTATAAGTGG CTGCCAGTGT ACAATGCGGA AGTGGTAGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCT CCACCCCACA TCTTCTCCAT CTCTGACAAC 
GCCTATCAGT ACATGCTGAC AGATCGGGAG AACCAGTCCA TCCTCATCAC CGGAGAATCC 
GGAGCTGGTA AGACTGTCAA CACCAAGAGG GTCATCCAAT ATTTTGCTGT TATTGCTGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGACC CCAGGCAAGG GCACCTTGGA AGATCAAATC 
ATCCAAGCCA ACCCCGCTCT GGAGGCCTTT GGCAATGCCA AGACAGTTCG GAATGATAAC 
TCCTCCCGAT TTGGGAAATT CATTCGAATC CATTTTGGAG CAACAGGAAA GTTGGCATCT 
GCAGATATAG AGACCTACCT TCTGGAAAAA TCCAGGGTTA TTTTCCAGCT GAAAGCAGAA 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAATAAAA AGCCTGAGCT TCTAGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCGTTCATCT CCCAGGGAGA GACGACTGTG 
GCCTCAATAG ATGACTCTGA AGAGCTCATG GCCACCGATA GCGCCTTTGA TGTGCTGGGC 
TTCACTCCAG AAGAGAAGAA CTCCATTTAC AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAACAGAA GCAGAGGGAG GAGCAGGCAG AGCCAGACGG CACGGAAGAA 
GCTGACAAGT CAGCCTACCT CATGGGACTG AACTCGGCTG ACCTGCTCAA GGGGTTGTGC 
CACCCTCGAG TCAAAGTGGG CAACGAGTAT GTCACCAAAG GGCAGAATGT CCAGCAGGTG 
GCATATGCCA TCGGGGCACT GGCCAAGTCA GTGTACGAGA AGATGTTCAA CTGGATGGTG 
ACACGCATCA ACGCAACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGTGTCCTG 
GACATCGCCG GCTTTGAGAT CTTTGATTTC AACAGCTTTG AGCAGCTGTG CATCAACTTC 
ACCAATGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TCGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AAGGCATCGA GTGGACGTTT ATTGACTTCG GCATGGACCT GCAGGCCTGC 
ATCGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAGGAGGA GTGCATGTTC 
CCCAAGGCCA CGGACATGAC CTTCAAGGCC AAGCTGTACG ACAACCACCT GGGCAAGTCC 
AACAACTTCC AGAAGCCTCG CAATATCAAG GGAAAGCAGG AAGCCCACTT CTCTCTGATC 
CACTATGCTG GCACCGTGGA CTACAATATC CTGGGCTGGC TACAGAAGAA CAAGGACCCT 
CTCAATGAGA CGGTGGTGGG GCTGTACCAG AAGTCCTCCC TCAAGCTCCT AAGTAATCTG 
TTTGCCAACT ATGCTGGAGC TGATGCACCT GTAGACAAGG GCAAAGGCAA AGCAAAGAAA 
GGCTCATCCT TTCAGACCGT GTCCGCACTG CACAGGGAAA ATCTGAACAA ACTTATGACA 
AACCTGCGCT CCACGCACCC TCACTTTGTA CGCTGCATCA TCCCCAATGA GACGAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GATGCAACGG AGTGCTGGAG 
GGTATCCGCA TCTGTAGGAA GGGCTTCCCC AACCGCATTC TTTATGGGGA CTTCCGGCAG 
AGGTATCGAA TCCTGAACCC AGCAGCCATC CCTGAGGGCC AATTCATTGA TAGCCGGAAA 
GGGGCTGAGA AGCTGCTGGG CTCCCTGGAC ATTGACCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC GGGGCTGCTG GGGCTGCTGG AGGAGATGCG AGATGAGAGG 
CTGAGCCGCA TCATCACCAG AATCCAGGCC CAGTCCCGAG GTGTACTTTC CAGAATGGAG 
TTTAAGAAGC TGCTGGAGCG CAGAGACTCC CTGCTGATTA TCCAGTGGAA CATCCGCGCC 
TTCATGGGGG TCAAGAATTG GCCGTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGCGCAG AGACAGAGAA GGAGATGGCC AACATGAAGG AGGAGTTCGG GCGAGTCAAA 
GATGCACTAG AGAAGTCTGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCCGAGGAGC GCTGCGACCA GCTGATCAAG AACAAGATCC AGCTGGAGGC CAAGGTGAAG 
GAGATGACCG AGAGGCTGGA GGACGAGGAG GAGATGAACG CCGAGCTCAC GGCCAAGAAG 
CGCAAACTGG AAGACGAGTG CTCAGAGCTC AAGAGAGATA TCGATGACCT GGAGCTGACC 
CTGGCCAAGG TGGAGAAGGA AAAGCACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGACGA GATCATTGTC AAGCTGACCA AGGAGAAGAA AGCTCTACAA 
GAGGCCCACC AGCAAGCCCT AGATGACCTT CAGGCTGAGG AGGACAAGGT CAACACTCTG 
ACCAAGGCCA AGGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAGGGATC CCTGGAGCAG 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCAAAGCGGA AGCTGGAGGG TGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AACGACAAGC AGCAGTTGGA TGAGCGACTC 
AAAAAGAAGG ACTTTGAGTT AAATGCACTC AACGCCAGGA TTGAGGATGA GCAGGCCCTG 
GGCAGCCAGC TGCAGAAGAA GCTCAAAGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CTGAGCGCAC AGCCCGGGCC AAGGTGGAGA AGCTGCGCTC AGACCTGTCC 
CGGGAGCTGG AGGAGATCAG TGAGAGGCTA GAGGAAGCCG GTGGGGCCAC ATCTGTGCAG 
ATAGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTAGAGGAG 
GCCACGCTGC AGCATGAGGC CACAGCTGCG GCCCTGCGCA AGAAACACGC GGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT AGACAATCTA CAGCGGGTGA AGCAGAAGCT GGAGAAAGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTTACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCCTGGAGG ACCAGATGAA TGAACACCGG 
AGCAAGGCTG AGGAGACACA GCGTTCTGTC AATGACCTCA CCAGCCAGCG GGCCAAGCTG 
CAGACAGAGA ATGGGGAGCT GTCCAGACAG CTGGATGAGA AGGAGGCTCT TATCTCTCAG 
CTGACCCGAG GCAAGCTCAC GTATACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTCA AGGCCAAGAA TGCCCTGGCC CACGCACTGC AGTCAGCCCG GCATGATTGC 
GACCTGCTGC GGGAACAGTA TGAGGAGGAA ACAGAAGCCA AGGCTGAGCT GCAGCGTGTC 
CTGTCCAAGG CCAACTCAGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATA 
CAGAGGACGG AGGAGCTGGA GGAAGCCAAG AAGAAGCTGG CTCAGAGGCT TCAGGATGCT 
GAGGAGGCAG TGGAGGCCGT CAACGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACAGG 
CTGCAGAACG AGATCGAGGA CCTGATGGTG GATGTGGAGC GCTCCAATGC GGCCGCCGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CTGAGTGGAA GCAGAAGTAT 
GAGGAGTCCC AGTCAGAGCT GGAGTCTTCC CAGAAGGAGG CGCGCTCCCT GAGCACAGAG 
CTCTTCAAGC TCAAGAATGC CTATGAGGAG TCTCTGGAGC ACCTGGAGAC CTTCAAGCGG 
GAGAACAAGA ACCTCCAGGA GGAGATCTCA GACCTGACTG AACAGCTGGG CTCAACTGGG 
AAGAGCATCC ACGAGCTGGA GAAGATCCGA AAGCAACTGG AGGCTGAGAA GCTGGAGCTG 
CAGTCAGCCC TGGAAGAGGC TGAGGCCTCC CTGGAGCATG AGGAGGGCAA GATCCTCCGA 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAAA GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AGCAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCCCT GCAGACCTCC 
CTGGATGCCG AGACGCGCAG CCGCAACGAG GCCCTGCGGG TGAAGAAGAA GATGGAGGGC 
GACCTCAACG AGATGGAGAT CCAGCTCAGT CATGCCAACC GCATGGCTGC TGAGGCCCAG 
AAACAAGTGA AGAGCCTCCA GAGTTTGCTG AAGGACACTC AAATCCAGCT GGATGACGCA 
GTCCGTGCCA ATGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCGG AGCTGGAGGA GCTGCGGGCC GTGGTGGAGC AGACGGAGCG GTCTCGGAAG 
CTGGCAGAGC AGGAGCTGAT CGAGACCAGC GAGCGGGTGC AGCTGCTGCA CTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GACGCAGACC TCTCCCAGCT CCAGACAGAG 
GTGGAGGAGG CGGTGCAGGA GTGTAGGAAC GCAGAGGAGA AGGCCAAGAA GGCCATCACA 
GATGCCGCCA TGATGGCCGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA GCAGACCATC AAGGACCTGC AGCACCGGCT GGACGAGGCA 
GAGCAGATCG CCCTCAAGGG TGGCAAGAAG CAGCTGCAGA AGCTGGAGGC CCGGGTCCGG 
GAGCTGGAGA ATGAGCTGGA GGCTGAGCAG AAGCGCAATG CGGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CAGAGGAAGA CAGGAAGAAC 
CTACTGCGAC TGCAGGACCT GGTGGACAAG CTGCAGTTAA AGGTGAAGGC CTACAAGCGC 
CAGGCTGAGG AGGCGGAGGA ACAGGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGAGGGCG GACATTGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCGCCAAG GTGGGTTCCT GCCCTCGAAT TACCAGTCAT 
GCTCTCACCA GGATACACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impact of MYH6 variants in hypoplastic left heart syndrome.
Physiological genomics48(12)912-921(2016 Dec)
Tomita-Mitchell A,Stamm KD,Mahnke DK,Kim MS,Hidestrand PM,Liang HL,Goetsch MA,Hidestrand M,Simpson P,Pelech AN,Tweddell JS,Benson DW,Lough JW,Mitchell ME

Rat cardiac troponin T mutation (F72L)-mediated impact on thin filament cooperativity is divergently modulated by ?- and ?-myosin heavy chain isoforms.
American journal of physiology. Heart and circulatory physiology309(8)H1260-70(2015 Oct)
Chandra V,Gollapudi SK,Chandra M

l-Arginine Attenuates Cardiac Dysfunction, But Further Down-Regulates ?-Myosin Heavy Chain Expression in Isoproterenol-Induced Cardiomyopathy.
Basic & clinical pharmacology & toxicology117(4)251-60(2015 Oct)
Kralova E,Doka G,Pivackova L,Srankova J,Kuracinova K,Janega P,Babal P,Klimas J,Krenek P

Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly.
Proceedings of the National Academy of Sciences of the United States of America112(29)E3806-15(2015 Jul)
Taylor KC,Buvoli M,Korkmaz EN,Buvoli A,Zheng Y,Heinze NT,Cui Q,Leinwand LA,Rayment I

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Myh7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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