MYH7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH7
Entrez Gene ID	4625
Full Name	myosin heavy chain 7
Synonyms	CMD1S,CMH1,MPD1,MYHCB,SPMD,SPMM
General protein information	Preferred Names myosin heavy chain 7 Names myosin-7 cardiac muscle myosin heavy chain 7 beta myHC-beta myhc-slow myopathy, distal 1 myosin 7 myosin heavy chain beta-subunit myosin heavy chain slow isoform myosin heavy chain, cardiac muscle beta isoform myosin, heavy chain 7, cardiac muscle, beta myosin, heavy polypeptide 7, cardiac muscle, beta rhabdomyosarcoma antigen MU-RMS-40.7A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2
Summary	Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008].
Disorder MIM:	160760
Disorder Html:	Cardiomyopathy, hypertrophic, 1, 192600 (3); Cardiomyopathy, dilated, 1S, 613426 (3); Myopathy, myosin storage, autosomal dominant, 608358 (3); Laing distal myopathy, 160500 (3); Scapuloperoneal syndrome, myopathic type, 181430 (3); Left ventricular noncompaction 5, 613426 (3); Myopathy, myosin storage, autosomal recessive, 255160 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000257.3	NP_000248.2	myosin-7
XM_017021340.1	XP_016876829.1	myosin-7 isoform X1
NM_000257.4	NP_000248.2	myosin-7

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	smyhc1	NP_001018343.1
Danio rerio (zebrafish)	vmhcl	NP_001070932.2
Drosophila melanogaster (fruit fly)	Mhc	NP_001246049.1
Rattus norvegicus (Norway rat)	Myh7	NP_058936.1
Danio rerio (zebrafish)	LOC100329748	XP_002666778.1
Eremothecium gossypii	AGOS_ACR068W	NP_983470.2
Homo sapiens (human)	MYH7	NP_000248.2
Canis lupus familiaris (dog)	MYH7	NP_001107183.1
Mus musculus (house mouse)	Myh7	NP_542766.1
Danio rerio (zebrafish)	vmhc	NP_001106204.1
Danio rerio (zebrafish)	si:ch211-24n20.3	XP_001923213.1
Danio rerio (zebrafish)	smyhc2	NP_001096096.2
Danio rerio (zebrafish)	LOC100329813	XP_002666779.1
Saccharomyces cerevisiae (baker's yeast)	MYO1	NP_011888.1
Kluyveromyces lactis	KLLA0E11595g	XP_454473.1
Pan troglodytes (chimpanzee)	MYH7	XP_001150786.3
Bos taurus (cattle)	MYH7	NP_777152.1
Danio rerio (zebrafish)	LOC100329670	XP_002666777.2
Danio rerio (zebrafish)	LOC567740	XP_696132.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010147	XP_319309.3
Xenopus tropicalis (tropical clawed frog)	myh7	XP_002939061.1
Gallus gallus (chicken)	MYH7	NP_001001302.1
Danio rerio (zebrafish)	smyhc3	NP_001129995.1
Schizosaccharomyces pombe (fission yeast)	myo2	NP_588114.1

Gene Ontology
Bibliography

Related articles in PubMed

[Analysis of genotype and phenotype correlation of MYH7-V878A mutation among ethnic Han Chinese pedigrees affected with hypertrophic cardiomyopathy].
Wang B, Guo R, Zuo L, Shao H, Liu Y, Wang Y, Ju Y, Sun C, Wang L, Zhang Y, Liu L
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(4)514-518(2017 Aug)

Hypertrophic cardiomyopathy clinical phenotype is independent of gene mutation and mutation dosage.
Viswanathan SK, Sanders HK, McNamara JW, Jagadeesan A, Jahangir A, Tajik AJ, Sadayappan S
PloS one12(11)e0187948(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Double MYH7 CTTNA3 heterozygotes showed a variable clinical expression of arrhythmogenic cardiomyopathy and hypertrophic cardiomyopathy. One carrier of double mutations in CTTNA3 and MYH7 genes did not fulfill the current diagnostic criteria for cardiomyopathy.
Title: Co-inheritance of mutations associated with arrhythmogenic cardiomyopathy and hypertrophic cardiomyopathy.

Digenic inheritance of two novel variants in TNNT2 and MYH7 is associated with a severe form of dilated cardiomyopathy.
Title: Digenic inheritance of mutations in the cardiac troponin (TNNT2) and cardiac beta myosin heavy chain (MYH7) as the cause of severe dilated cardiomyopathy.

MYH7-V878A is a hot spot among ethnic Han Chinese with a high penetrance.
Title: [Analysis of genotype and phenotype correlation of MYH7-V878A mutation among ethnic Han Chinese pedigrees affected with hypertrophic cardiomyopathy].

The authors demonstrate myosin tail (S2)-dependent functional regulation of actin-activated human beta-cardiac myosin ATPase. In addition, they show that both S2 and MyBP-C bind to S1 and that phosphorylation of either S1 or MyBP-C weakens these interactions.
Title: The myosin mesa and the basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.

Data provide evidence that MYH7 mutations contributed to 24.4% MYBPC3 mutations of hypertrophic cardiomyopathy (HCM) cases, that MYBPC3 constitute the preeminent cause of HCM and that both mutations are phenotypically indistinguishable.
Title: Hypertrophic cardiomyopathy clinical phenotype is independent of gene mutation and mutation dosage.

The following MYH7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28301	NM_000257.4 Latest version!	Homo sapiens myosin heavy chain 7 (MYH7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu28301	XM_017021340.1 Latest version!	Homo sapiens myosin heavy chain 7 (MYH7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
View Old Accession Versions >>
OHu28301	NM_000257.3	Homo sapiens myosin heavy chain 7 (MYH7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH7 ( NM_000257.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000248.2 Homo sapiens myosin heavy chain 7 (MYH7), mRNA.

Price & Availability↑

CloneID	OHu28301
Clone ID Related Accession (Same CDS sequence)	NM_000257.3 , XM_017021340.1 , NM_000257.4
Accession Version	NM_000257.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	myosin-7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF834726.1, EU747717.1, M58018.1 and BC112173.1. On or before Jun 20, 2014 this sequence version replaced XM_005267696.1, NM_000257.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU747717.1, M58018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT 
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT 
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG 
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC 
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC 
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC 
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC 
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC 
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG 
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC 
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG 
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC 
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG 
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG 
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG 
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC 
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC 
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC 
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC 
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC 
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT 
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG 
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA 
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC 
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG 
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG 
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG 
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG 
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG 
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC 
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA 
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG 
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG 
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA 
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA 
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG 
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG 
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC 
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT 
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG 
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG 
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG 
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG 
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG 
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC 
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC 
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT 
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT 
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG 
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT 
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG 
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG 
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA 
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG 
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC 
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA 
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG 
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA 
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG 
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA 
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG 
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC 
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG 
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC 
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC 
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000248.2
CDS	132..5939
Translation	MGDSEMAVFGAAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE

Target ORF information:

RefSeq Version	NM_000257.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7 (MYH7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000257.3

ORF Insert Sequence:

ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT 
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT 
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG 
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC 
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC 
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC 
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC 
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC 
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG 
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC 
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG 
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC 
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG 
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG 
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG 
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC 
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC 
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC 
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC 
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC 
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT 
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG 
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA 
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC 
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG 
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG 
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG 
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG 
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG 
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC 
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA 
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG 
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG 
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA 
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA 
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG 
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG 
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC 
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT 
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG 
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG 
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG 
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG 
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG 
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC 
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC 
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT 
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT 
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG 
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT 
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG 
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG 
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA 
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG 
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC 
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA 
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG 
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA 
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG 
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA 
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG 
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC 
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG 
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC 
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC 
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7 ( NM_000257.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000248.2 Homo sapiens myosin heavy chain 7 (MYH7), mRNA.

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CloneID	OHu28301
Clone ID Related Accession (Same CDS sequence)	NM_000257.3 , XM_017021340.1 , NM_000257.4
Accession Version	NM_000257.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	myosin-7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU747717.1, M58018.1 and BC112173.1. On Nov 22, 2018 this sequence version replaced NM_000257.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M58018.1, EU747717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355349.4/ ENSP00000347507.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT 
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT 
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG 
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC 
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC 
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC 
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC 
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC 
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG 
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC 
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG 
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC 
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG 
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG 
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG 
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC 
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC 
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC 
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC 
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC 
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT 
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG 
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA 
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC 
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG 
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG 
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG 
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG 
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG 
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC 
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA 
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG 
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG 
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA 
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA 
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG 
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG 
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC 
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT 
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG 
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG 
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG 
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG 
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG 
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC 
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC 
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT 
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT 
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG 
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT 
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG 
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG 
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA 
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG 
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC 
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA 
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG 
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA 
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG 
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA 
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG 
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC 
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG 
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC 
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC 
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000248.2
CDS	106..5913
Translation	MGDSEMAVFGAAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE

Target ORF information:

RefSeq Version	NM_000257.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7 (MYH7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000257.4

ORF Insert Sequence:

ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT 
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT 
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG 
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC 
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC 
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC 
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC 
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC 
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG 
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC 
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG 
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC 
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG 
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG 
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG 
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC 
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC 
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC 
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC 
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC 
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT 
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG 
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA 
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC 
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG 
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG 
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG 
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG 
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG 
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC 
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA 
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG 
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG 
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA 
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA 
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG 
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG 
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC 
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT 
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG 
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG 
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG 
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG 
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG 
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC 
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC 
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT 
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT 
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG 
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT 
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG 
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG 
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA 
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG 
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC 
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA 
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG 
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA 
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG 
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA 
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG 
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC 
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG 
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC 
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC 
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH7 ( XM_017021340.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876829.1 Homo sapiens myosin heavy chain 7 (MYH7), transcript variant X1, mRNA.

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CloneID	OHu28301
Clone ID Related Accession (Same CDS sequence)	NM_000257.3 , XM_017021340.1 , NM_000257.4
Accession Version	XM_017021340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT 
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT 
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG 
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC 
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC 
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC 
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC 
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC 
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG 
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC 
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG 
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC 
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG 
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG 
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG 
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC 
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC 
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC 
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC 
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC 
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT 
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG 
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA 
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC 
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG 
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG 
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG 
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG 
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG 
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC 
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA 
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG 
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG 
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA 
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA 
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG 
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG 
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC 
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT 
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG 
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG 
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG 
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG 
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG 
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC 
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC 
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT 
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT 
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG 
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT 
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG 
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG 
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA 
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG 
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC 
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA 
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG 
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA 
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG 
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA 
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG 
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC 
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG 
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC 
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC 
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876829.1
CDS	67..5874
Translation	MGDSEMAVFGAAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE

Target ORF information:

RefSeq Version	XM_017021340.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 7 (MYH7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021340.1

ORF Insert Sequence:

ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT 
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT 
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC 
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG 
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC 
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT 
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC 
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC 
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC 
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC 
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC 
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT 
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG 
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG 
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG 
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC 
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA 
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG 
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC 
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG 
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG 
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG 
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC 
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG 
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC 
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC 
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC 
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC 
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT 
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG 
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA 
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC 
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT 
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG 
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG 
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG 
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC 
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG 
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG 
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC 
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG 
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA 
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG 
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT 
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG 
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG 
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA 
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG 
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA 
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG 
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA 
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG 
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG 
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC 
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG 
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT 
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG 
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG 
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG 
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG 
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG 
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG 
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG 
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG 
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG 
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC 
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC 
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT 
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT 
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG 
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA 
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT 
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG 
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG 
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA 
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG 
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG 
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC 
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC 
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA 
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG 
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA 
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG 
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG 
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC 
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA 
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG 
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG 
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC 
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG 
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG 
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC 
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC 
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG 
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG 
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Analysis of genotype and phenotype correlation of MYH7-V878A mutation among ethnic Han Chinese pedigrees affected with hypertrophic cardiomyopathy].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(4)514-518(2017 Aug)
Wang B,Guo R,Zuo L,Shao H,Liu Y,Wang Y,Ju Y,Sun C,Wang L,Zhang Y,Liu L

Hypertrophic cardiomyopathy clinical phenotype is independent of gene mutation and mutation dosage.
PloS one12(11)e0187948(2017)
Viswanathan SK,Sanders HK,McNamara JW,Jagadeesan A,Jahangir A,Tajik AJ,Sadayappan S

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MYH7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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