Mhc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mhc
Entrez Gene ID	35007
Full Name	Myosin heavy chain
Synonyms	Bsh,CG17927,DROMHC,Dm II,DmMHC,Dmel\CG17927,DroMII,FBgn0002741,Ifm(2)2,Ifm(2)RU1,MHC,MRP,Mhc36B,Mrp,MyHC,Myo,Myo6,Nup,Sht,Stp,chr2L:16765388..16765564,chr2L:16781880..16782040,ifm(2)2,ifm(2)RU1,ifm(2)RU2,l(2)36Ae,l(2)M66,l(2)k10423,mMHC,mhc,sMHC
General protein information	Preferred Names Myosin heavy chain Names CG17927 gene product from transcript CG17927-RK CG17927-PA CG17927-PB CG17927-PC CG17927-PD CG17927-PE CG17927-PF CG17927-PG CG17927-PH CG17927-PI CG17927-PK CG17927-PL CG17927-PM CG17927-PN CG17927-PO CG17927-PP CG17927-PQ CG17927-PR CG17927-PS CG17927-PT CG17927-PU CG17927-PV Mhc-PA Mhc-PB Mhc-PC Mhc-PD Mhc-PE Mhc-PF Mhc-PG Mhc-PH Mhc-PI Mhc-PK Mhc-PL Mhc-PM Mhc-PN Mhc-PO Mhc-PP Mhc-PQ Mhc-PR Mhc-PS Mhc-PT Mhc-PU Mhc-PV Not-upheld Shrunken-thorax beta-myosin heavy chain chain heavy chain indirect flight muscle (2) RU1 indirect flight muscle (2) RU2 muscle myosin muscle myosin II muscle myosin II heavy chain muscle myosin heavy chain muscle myosin-II muscle specific myosin II myo-II heavy chain myosin myosin heavy myosin heavy Chain-PF myosin heavy chain myosin heavy-chain myosin heavy-chain protein myosin rod protein myosin-heavy chain samba sarcomeric myosin heavy-chain shrunken thorax stuckup
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36A8-36B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165181.3	NP_723999.1	myosin heavy chain, isoform C
NM_165182.3	NP_724000.1	myosin heavy chain, isoform G
NM_165184.3	NP_724002.2	myosin heavy chain, isoform Q
NM_001259122.1	NP_001246051.1	myosin heavy chain, isoform U
NM_001259119.2	NP_001246048.1	myosin heavy chain, isoform R
NM_001169519.2	NP_001162990.1	myosin heavy chain, isoform N
NM_165188.3	NP_724006.1	myosin heavy chain, isoform I
NM_078863.6	NP_523587.4	myosin heavy chain, isoform H
NM_165183.3	NP_724001.1	myosin heavy chain, isoform E
NM_165190.4	NP_724008.1	myosin heavy chain, isoform K
NM_165192.4	NP_724010.1	myosin heavy chain, isoform M
NM_001259120.1	NP_001246049.1	myosin heavy chain, isoform S
NM_001169521.2	NP_001162992.1	myosin heavy chain, isoform P
NM_165191.3	NP_724009.1	myosin heavy chain, isoform L
NM_165185.3	NP_724003.1	myosin heavy chain, isoform F
NM_165186.3	NP_724004.1	myosin heavy chain, isoform D
NM_165187.3	NP_724005.1	myosin heavy chain, isoform A
NM_165189.3	NP_724007.1	myosin heavy chain, isoform B
NM_001169520.3	NP_001162991.1	myosin heavy chain, isoform O
NM_001259121.2	NP_001246050.1	myosin heavy chain, isoform T
NM_001316398.1	NP_001303327.1	myosin heavy chain, isoform V

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	smyhc1	NP_001018343.1
Danio rerio (zebrafish)	vmhcl	NP_001070932.2
Drosophila melanogaster (fruit fly)	Mhc	NP_001246049.1
Rattus norvegicus (Norway rat)	Myh7	NP_058936.1
Danio rerio (zebrafish)	LOC100329748	XP_002666778.1
Eremothecium gossypii	AGOS_ACR068W	NP_983470.2
Homo sapiens (human)	MYH7	NP_000248.2
Canis lupus familiaris (dog)	MYH7	NP_001107183.1
Mus musculus (house mouse)	Myh7	NP_542766.1
Danio rerio (zebrafish)	vmhc	NP_001106204.1
Danio rerio (zebrafish)	si:ch211-24n20.3	XP_001923213.1
Danio rerio (zebrafish)	smyhc2	NP_001096096.2
Danio rerio (zebrafish)	LOC100329813	XP_002666779.1
Saccharomyces cerevisiae (baker's yeast)	MYO1	NP_011888.1
Kluyveromyces lactis	KLLA0E11595g	XP_454473.1
Pan troglodytes (chimpanzee)	MYH7	XP_001150786.3
Bos taurus (cattle)	MYH7	NP_777152.1
Danio rerio (zebrafish)	LOC100329670	XP_002666777.2
Danio rerio (zebrafish)	LOC567740	XP_696132.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010147	XP_319309.3
Xenopus tropicalis (tropical clawed frog)	myh7	XP_002939061.1
Gallus gallus (chicken)	MYH7	NP_001001302.1
Danio rerio (zebrafish)	smyhc3	NP_001129995.1
Schizosaccharomyces pombe (fission yeast)	myo2	NP_588114.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that MHC isoforms can alter FSA and hence cyclical power generation but that isoforms can only endow a muscle type with moderate FSA Highly SA muscle types, such as IFM, likely use a different or additional mechanism.
Title: The Drosophila indirect flight muscle myosin heavy chain isoform is insufficient to transform the jump muscle into a highly stretch-activated muscle type.

Unipolar distributions of junctional Myosin II identify cell stripe boundaries that drive cell intercalation throughout Drosophila axis extension.
Title: Unipolar distributions of junctional Myosin II identify cell stripe boundaries that drive cell intercalation throughout Drosophila axis extension.

Introduction of a mutation (R759E) in the converter domain inhibits the steady-state ATPase of myosin S1, whereas an additional mutation in the relay domain (N509K) is able to restore the ATPase.
Title: The Relay/Converter Interface Influences Hydrolysis of ATP by Skeletal Muscle Myosin II.

The vector of cell movement is regulated by localised epidermal growth factor (EGF) signalling from the distally placed tip cell lineage and the acquisition of planar polarity leads to asymmetric pulsatile Myosin II accumulation.
Title: Epidermal growth factor signalling controls myosin II planar polarity to orchestrate convergent extension movements during Drosophila tubulogenesis.

Data indicate that disruption of the relay-converter domain interface leads to complete absence of myosin ATPase activity.
Title: Mapping interactions between myosin relay and converter domains that power muscle function.

The following Mhc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mhc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa41349	NM_001316398.1 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant V (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OFa14777	NM_165191.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant L (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14778	NM_165181.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant C (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14779	NM_165182.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant G (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14779	NM_001169520.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant O (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14780	NM_165183.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant E (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14781	NM_165185.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant F (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14782	NM_165186.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant D (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14783	NM_165187.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant A (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14784	NM_165188.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant I (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14785	NM_078863.6 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant H (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14786	NM_165190.4 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant K (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14787	NM_165192.4 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant M (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14788	NM_165189.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant B (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa14789	NM_165184.3 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant Q (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16842	NM_001259119.2 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant R (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16843	NM_001169519.2 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant N (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16844	NM_001169521.2 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant P (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16845	NM_001259122.1 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant U (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16846	NM_001259120.1 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant S (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa16847	NM_001259121.2 Latest version!	Drosophila melanogaster myosin heavy chain, transcript variant T (Mhc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mhc ( NM_001316398.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303327.1 Drosophila melanogaster myosin heavy chain, transcript variant V (Mhc), mRNA.

Price & Availability↑

CloneID	OFa41349
Clone ID Related Accession (Same CDS sequence)	NM_001316398.1
Accession Version	NM_001316398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform V
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGGTA 
TTTCTGGGCC AACATATTGG TGATTATCCC GGCATTTGCC AGGGCAAAAC ACGGATACCC 
GGTGTCAACG ATGGGGAAGA ATTTGAGTTA ACTGACCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303327.1
CDS	276..6164
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEMVFLGQHIGDYPGICQGKTRIPGVNDGEEFELTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_001316398.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant V (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316398.1

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGGTA 
TTTCTGGGCC AACATATTGG TGATTATCCC GGCATTTGCC AGGGCAAAAC ACGGATACCC 
GGTGTCAACG ATGGGGAAGA ATTTGAGTTA ACTGACCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165191.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724009.1 Drosophila melanogaster myosin heavy chain, transcript variant L (Mhc), mRNA.

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CloneID	OFa14777
Clone ID Related Accession (Same CDS sequence)	NM_165191.3
Accession Version	NM_165191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165191.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724009.1
CDS	121..5931
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTALDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAI

Target ORF information:

RefSeq Version	NM_165191.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant L (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165191.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165181.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723999.1 Drosophila melanogaster myosin heavy chain, transcript variant C (Mhc), mRNA.

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CloneID	OFa14778
Clone ID Related Accession (Same CDS sequence)	NM_165181.3
Accession Version	NM_165181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165181.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723999.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165181.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant C (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165181.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165182.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724000.1 Drosophila melanogaster myosin heavy chain, transcript variant G (Mhc), mRNA.

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CloneID	OFa14779
Clone ID Related Accession (Same CDS sequence)	NM_165182.3 , NM_001169520.3
Accession Version	NM_165182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165182.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724000.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165182.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant G (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165182.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001169520.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162991.1 Drosophila melanogaster myosin heavy chain, transcript variant O (Mhc), mRNA.

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CloneID	OFa14779
Clone ID Related Accession (Same CDS sequence)	NM_165182.3 , NM_001169520.3
Accession Version	NM_001169520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform O
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169520.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162991.1
CDS	283..6171
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_001169520.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant O (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169520.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165183.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724001.1 Drosophila melanogaster myosin heavy chain, transcript variant E (Mhc), mRNA.

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CloneID	OFa14780
Clone ID Related Accession (Same CDS sequence)	NM_165183.3
Accession Version	NM_165183.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165183.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724001.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165183.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant E (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165183.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165185.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724003.1 Drosophila melanogaster myosin heavy chain, transcript variant F (Mhc), mRNA.

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CloneID	OFa14781
Clone ID Related Accession (Same CDS sequence)	NM_165185.3
Accession Version	NM_165185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165185.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGGTACC AGATCCTGAA CCCCCGTGGC ATTAAGGACC TCGATTGTCC CAAGAAAGCC 
TCCAAGGTTC TGATTGAGTC CACCGAGCTG AACGAAGACC TTTACCGTCT GGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724003.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPRGIKDLDCPKKASKVLIESTELNEDLYRLGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165185.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant F (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165185.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGGTACC AGATCCTGAA CCCCCGTGGC ATTAAGGACC TCGATTGTCC CAAGAAAGCC 
TCCAAGGTTC TGATTGAGTC CACCGAGCTG AACGAAGACC TTTACCGTCT GGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165186.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724004.1 Drosophila melanogaster myosin heavy chain, transcript variant D (Mhc), mRNA.

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CloneID	OFa14782
Clone ID Related Accession (Same CDS sequence)	NM_165186.3
Accession Version	NM_165186.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165186.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724004.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165186.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant D (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165186.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165187.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724005.1 Drosophila melanogaster myosin heavy chain, transcript variant A (Mhc), mRNA.

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CloneID	OFa14783
Clone ID Related Accession (Same CDS sequence)	NM_165187.3
Accession Version	NM_165187.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165187.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724005.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165187.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant A (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165187.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165188.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724006.1 Drosophila melanogaster myosin heavy chain, transcript variant I (Mhc), mRNA.

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CloneID	OFa14784
Clone ID Related Accession (Same CDS sequence)	NM_165188.3
Accession Version	NM_165188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165188.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724006.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165188.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant I (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165188.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_078863.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523587.4 Drosophila melanogaster myosin heavy chain, transcript variant H (Mhc), mRNA.

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CloneID	OFa14785
Clone ID Related Accession (Same CDS sequence)	NM_078863.6
Accession Version	NM_078863.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078863.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523587.4
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQLCIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_078863.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant H (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078863.6

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAAC AATTGTGCAT CAATTTCACT 
AACGAAAAAC TGCAACAATT CTTCAATCAT CATATGTTCG TTTTGGAACA AGAAGAATAC 
CAACGCGAGG GCATCGAATG GACCTTCATT GATTTCGGCA TGGATCTGCA ATTGTGTATT 
GATCTGATCG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165190.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724008.1 Drosophila melanogaster myosin heavy chain, transcript variant K (Mhc), mRNA.

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CloneID	OFa14786
Clone ID Related Accession (Same CDS sequence)	NM_165190.4
Accession Version	NM_165190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165190.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724008.1
CDS	289..6099
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAI

Target ORF information:

RefSeq Version	NM_165190.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant K (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165190.4

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGCTTCGAGC AATTGTGTAT TAACTTCACC 
AATGAGAAAC TGCAGCAGTT CTTTAACCAT ATCATGTTCG TCATGGAGCA AGAGGAATAC 
AAGAAGGAGG GTATTAACTG GGACTTTATC GATTTCGGTA TGGACTTGCT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165192.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724010.1 Drosophila melanogaster myosin heavy chain, transcript variant M (Mhc), mRNA.

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CloneID	OFa14787
Clone ID Related Accession (Same CDS sequence)	NM_165192.4
Accession Version	NM_165192.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165192.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724010.1
CDS	121..5931
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTALDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAI

Target ORF information:

RefSeq Version	NM_165192.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant M (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165192.4

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165189.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724007.1 Drosophila melanogaster myosin heavy chain, transcript variant B (Mhc), mRNA.

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CloneID	OFa14788
Clone ID Related Accession (Same CDS sequence)	NM_165189.3
Accession Version	NM_165189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165189.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724007.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTALDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165189.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant B (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165189.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_165184.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724002.2 Drosophila melanogaster myosin heavy chain, transcript variant Q (Mhc), mRNA.

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CloneID	OFa14789
Clone ID Related Accession (Same CDS sequence)	NM_165184.3
Accession Version	NM_165184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform Q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_165184.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724002.2
CDS	263..6151
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_165184.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant Q (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165184.3

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001259119.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246048.1 Drosophila melanogaster myosin heavy chain, transcript variant R (Mhc), mRNA.

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CloneID	OFa16842
Clone ID Related Accession (Same CDS sequence)	NM_001259119.2
Accession Version	NM_001259119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform R
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259119.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246048.1
CDS	121..6009
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTALDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_001259119.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant R (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259119.2

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001169519.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162990.1 Drosophila melanogaster myosin heavy chain, transcript variant N (Mhc), mRNA.

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CloneID	OFa16843
Clone ID Related Accession (Same CDS sequence)	NM_001169519.2
Accession Version	NM_001169519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform N
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169519.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162990.1
CDS	121..5970
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	NM_001169519.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant N (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169519.2

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001169521.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162992.1 Drosophila melanogaster myosin heavy chain, transcript variant P (Mhc), mRNA.

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CloneID	OFa16844
Clone ID Related Accession (Same CDS sequence)	NM_001169521.2
Accession Version	NM_001169521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform P
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169521.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162992.1
CDS	121..5970
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEMCFLSDNIYDYYNVSQGKVTVPNMDDGEEFQLADQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPAGIVGVDDPKKCGSIILESTALDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	NM_001169521.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant P (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169521.2

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAGATGTGC 
TTCCTCTCCG ATAACATTTA CGACTACTAT AACGTATCCC AGGGTAAAGT AACTGTACCC 
AACATGGATG ACGGTGAGGA ATTCCAGCTT GCAGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTATC AAATCCTGAA CCCAGCTGGC ATTGTTGGCG TCGATGATCC CAAAAAGTGT 
GGATCCATCA TTTTGGAATC CACCGCATTG GATCCCGATA TGTATCGCAT TGGACACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001259122.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246051.1 Drosophila melanogaster myosin heavy chain, transcript variant U (Mhc), mRNA.

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CloneID	OFa16845
Clone ID Related Accession (Same CDS sequence)	NM_001259122.1
Accession Version	NM_001259122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform U
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246051.1
CDS	281..6130
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPKGIKGIEDPKKCTKVLIESTELNDDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPTASKGRKSALLEQ

Target ORF information:

RefSeq Version	NM_001259122.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant U (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259122.1

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGACATTTGT 
CTGTTGACCG ATAACATCTA CGATTACCAC ATTGTCTCCC AGGGCAAGGT CACTGTAGCC 
AGTATCGATG ATGCTGAGGA GTTCTCCCTC ACCGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACC AAATCCTGAA CCCCAAGGGT ATCAAGGGTA TTGAGGATCC CAAGAAATGC 
ACGAAGGTTC TGATCGAGTC GACCGAACTG AACGATGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCGAC AGCGTCAAAG 
GGCCGCAAGA GCGCGCTGCT GGAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001259120.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246049.1 Drosophila melanogaster myosin heavy chain, transcript variant S (Mhc), mRNA.

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CloneID	OFa16846
Clone ID Related Accession (Same CDS sequence)	NM_001259120.1
Accession Version	NM_001259120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246049.1
CDS	276..6164
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYKIMCPKLLQGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_001259120.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant S (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259120.1

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGGTACGA GATATCAAAT CCGAGAAGGT GGAAAAAGTC 
AATCCACCAA AATTCGAAAA AATTGAAGAT ATGGCCGACA TGACCGTGCT TAACACTCCT 
TGCGTTTTGC ACAATCTGCG TCAGCGTTAC TATGCTAAGC TCATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGCTACA AGATCATGTG CCCCAAGCTA TTGCAGGGCG TTGAGAAAGA CAAAAAGGCC 
ACTGAAATTA TCATTAAGTT TATCGATTTG CCCGAAGATC AGTACCGTTT GGGTAACACA 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA ACACGATCGC 
CAGACTTGCC ACAACGAGCT GAATCAGACT CGTACCGCCT GCGATCAGCT GGGTCGCGAT 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhc ( NM_001259121.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246050.1 Drosophila melanogaster myosin heavy chain, transcript variant T (Mhc), mRNA.

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CloneID	OFa16847
Clone ID Related Accession (Same CDS sequence)	NM_001259121.2
Accession Version	NM_001259121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain, isoform T
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259121.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246050.1
CDS	71..5959
Translation	MPKPVANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGETRDLKKDLLQQVNPPKYEKAEDMSNLTYLNDASVLHNLRQRYYNKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKEYCLLSNNIYDYRIVSQGKTTIPSVNDGEEWVAVDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYMILAPAIMAAEKVAKNAAGKCLEAVGLDPDMYRIGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHITNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRGASPAPRATSVRPQFDGLAFPPRFDLAPENEF

Target ORF information:

RefSeq Version	NM_001259121.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain, transcript variant T (Mhc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259121.2

ORF Insert Sequence:

ATGCCGAAGC CAGTCGCAAA TCAGGAGGAT GAGGATCCCA CCCCATACCT GTTCGTGTCT 
TTGGAGCAAA GGCGTATCGA TCAATCGAAA CCCTATGACT CGAAGAAGTC TTGCTGGATC 
CCCGACGAGA AGGAGGGTTA TCTCCTTGGT GAGATCAAGG CCACCAAGGG CGATATCGTC 
TCCGTTGGTC TGCAGGGTGG AGAGACACGA GACTTAAAGA AAGATCTGCT CCAGCAAGTG 
AACCCCCCGA AATACGAAAA AGCCGAGGAT ATGTCCAACT TGACATACCT TAACGATGCC 
TCTGTGCTCC ATAACTTGAG ACAGAGATAC TACAACAAGC TGATCTACAC CTACTCCGGT 
CTTTTCTGCG TTGCCATCAA TCCTTACAAG CGTTACCCCG TGTATACCAA CCGTTGCGCT 
AAGATGTACC GTGGCAAGCG CCGTAATGAG GTGCCACCCC ATATTTTCGC CATCTCTGAC 
GGTGCCTACG TCGACATGTT GACCAACCAC GTGAATCAAT CTATGTTGAT CACCGGTGAG 
TCTGGTGCCG GAAAGACTGA GAACACCAAG AAGGTCATTG CGTACTTCGC CACTGTTGGT 
GCCTCCAAGA AGACCGATGA GGCCGCCAAG AGCAAGGGCT CCCTGGAAGA TCAGGTTGTG 
CAGACCAACC CTGTGCTTGA GGCCTTCGGT AACGCCAAGA CCGTGCGTAA CGATAACTCC 
TCTCGTTTCG GTAAATTCAT CCGTATCCAC TTCGGACCCA CTGGTAAACT GGCTGGTGCT 
GATATTGAGA CCTATCTGCT GGAGAAGGCT CGTGTCATCT CCCAGCAGTC CCTGGAGCGT 
TCCTACCACA TCTTCTACCA GATCATGTCT GGCTCCGTTC CCGGTGTTAA AGAATACTGT 
CTGCTCTCGA ACAACATTTA CGACTATCGC ATTGTCTCGC AAGGCAAAAC GACCATACCC 
AGCGTCAACG ATGGCGAGGA ATGGGTCGCC GTGGATCAAG CCTTCGACAT CTTGGGCTTC 
ACCAAGCAGG AGAAGGAGGA CGTGTACAGG ATCACCGCCG CTGTCATGCA CATGGGTGGC 
ATGAAGTTCA AGCAACGTGG TCGCGAGGAG CAGGCTGAGC AGGACGGCGA GGAGGAGGGT 
GGCCGTGTGT CGAAGCTGTT CGGTTGCGAT ACCGCCGAGC TGTACAAGAA CTTGCTGAAG 
CCCCGCATCA AGGTCGGCAA CGAGTTCGTC ACCCAGGGCC GTAACGTCCA GCAGGTCACC 
AACTCGATCG GTGCCCTCTG CAAGGGTGTG TTCGATCGTC TGTTCAAGTG GCTGGTGAAG 
AAGTGTAACG AGACTCTGGA TACCCAGCAG AAGCGTCAGC ACTTCATTGG TGTACTGGAT 
ATTGCTGGTT TTGAGATCTT CGAGTACAAC GGTTTCGAGC AACTGTGTAT TAACTTCACC 
AACGAGAAGT TGCAACAATT CTTCAACCAT CACATGTTCG TTTTGGAGCA AGAAGAATAC 
AAGAGGGAAG GCATTGACTG GGCCTTCATC GATTTCGGTA TGGACTTGTT GGCCTGTATC 
GATCTGATTG AAAAGCCCAT GGGTATCTTG TCCATCCTGG AGGAAGAGTC TATGTTCCCC 
AAGGCCACCG ATCAGACCTT CTCGGAGAAG CTGACCAACA CCCATTTGGG CAAGTCGGCT 
CCATTCCAGA AGCCCAAGCC TCCAAAGCCC GGTCAGCAGG CTGCCCACTT CGCCATTGCC 
CATTATGCTG GTTGTGTGTC CTACAACATC ACCGGTTGGT TGGAGAAGAA CAAGGATCCT 
CTGAACGACA CCGTTGTCGA CCAGTTCAAG AAGTCGCAGA ACAAGCTGCT GATCGAAATC 
TTCGCCGATC ACGCCGGTCA GTCGGGTGGC GGTGAACAGG CCAAGGGAGG TCGTGGCAAG 
AAGGGCGGTG GCTTCGCTAC CGTCTCGTCG GCCTACAAGG AGCAGTTGAA CAGCTTGATG 
ACCACTCTGC GTTCGACCCA GCCTCACTTC GTCCGTTGCA TCATTCCCAA CGAAATGAAG 
CAGCCTGGCG TGGTTGATGC CCACTTGGTC ATGCACCAGC TGACCTGTAA CGGTGTGCTT 
GAAGGTATCC GTATTTGCCG TAAGGGCTTC CCCAACAGGA TGATGTACCC TGACTTCAAG 
ATGCGTTACA TGATTCTGGC TCCTGCCATC ATGGCGGCCG AAAAGGTGGC CAAGAATGCT 
GCCGGAAAGT GCTTGGAAGC CGTCGGACTG GATCCCGATA TGTACCGCAT TGGTCACACC 
AAGGTGTTCT TCCGCGCCGG TGTCCTGGGT CAGATGGAGG AGTTCCGTGA TGAGCGTCTG 
GGCAAGATCA TGTCCTGGAT GCAGGCATGG GCCCGTGGTT ACCTGTCCCG TAAGGGCTTC 
AAGAAGCTCC AGGAACAGCG CGTCGCCCTC AAGGTTGTCC AGCGCAATCT GCGCAAGTAC 
CTGCAGCTCC GTACCTGGCC CTGGTACAAA CTGTGGCAGA AGGTCAAGCC CCTCCTCAAC 
GTCAGCCGCA TCGAGGATGA GATTGCCCGT CTGGAGGAGA AGGCCAAGAA GGCTGAGGAA 
CTGCATGCCG CTGAAGTGAA GGTGCGCAAG GAGCTCGAGG CCCTCAACGC CAAGCTTTTG 
GCTGAGAAGA CCGCTCTGCT GGACTCCCTG TCCGGCGAGA AGGGTGCCCT GCAGGACTAC 
CAGGAGCGCA ACGCCAAGTT GACCGCCCAG AAGAACGACC TCGAGAACCA GCTGCGCGAT 
ATCCAAGAGC GCCTGACTCA GGAGGAGGAT GCCCGCAACC AGCTGTTCCA GCAGAAGAAG 
AAGGCCGATC AGGAGATCTC TGGCCTGAAG AAGGACATCG AGGATCTGGA ATTGAACGTC 
CAGAAGGCCG AGCAGGACAA GGCCACCAAG GATCACCAGA TCCGCAACTT GAACGACGAG 
ATCGCCCACC AGGATGAGCT CATCAACAAG TTGAACAAGG AGAAGAAGAT GCAGGGAGAG 
ACCAACCAGA AGACCGGTGA GGAGCTCCAG GCCGCCGAGG ACAAGATCAA CCACTTGAAC 
AAGGTTAAGG CCAAGCTCGA GCAGACCCTC GATGAACTGG AGGATTCGCT GGAGCGCGAG 
AAGAAGGTGC GCGGCGATGT TGAGAAGTCC AAGCGCAAGG TTGAGGGCGA CCTCAAGCTC 
ACCCAGGAGG CTGTTGCCGA TCTGGAGCGC AACAAGAAGG AGCTCGAGCA GACCATCCAG 
CGCAAGGACA AGGAGCTGTC CTCCATCACC GCCAAGCTCG AGGACGAGCA GGTCGTTGTT 
CTGAAGCACC AGCGCCAGAT CAAGGAACTG CAGGCCCGCA TCGAGGAGCT CGAGGAAGAG 
GTCGAGGCTG AGCGCCAGGC CCGCGCCAAG GCTGAGAAGC AGCGCGCCGA TCTGGCCCGC 
GAACTCGAGG AATTGGGCGA GCGTCTGGAG GAGGCTGGCG GTGCCACCTC TGCCCAGATT 
GAGCTCAACA AGAAGCGTGA GGCTGAGTTG AGCAAACTGC GTCGCGATCT TGAGGAGGCC 
AACATCCAGC ACGAGTCCAC CCTGGCTAAC CTGCGCAAGA AGCACAACGA TGCCGTCGCC 
GAGATGGCCG AGCAGGTTGA TCAGCTCAAC AAGCTGAAGG CTAAGGCTGA GAAGGAGAAG 
AACGAGTACT ACGGCCAGTT GAACGATCTG CGCGCCGGTG TCGACCACAT TACCAACGAG 
AAGGCTGCCC AGGAGAAGAT CGCCAAGCAG CTGCAGCACA CCCTCAACGA GGTGCAGTCG 
AAACTGGACG AGACCAACAG GACTCTGAAC GACTTCGATG CCAGCAAGAA GAAGCTGTCC 
ATTGAGAACT CCGATCTGCT CCGCCAGCTG GAGGAGGCCG AGTCCCAGGT GTCTCAGCTG 
TCCAAGATCA AGATCTCTCT GACCACCCAG TTGGAGGACA CCAAGCGTCT GGCCGACGAG 
GAGTCGCGCG AGCGTGCCAC CCTTTTGGGC AAGTTCCGCA ACTTGGAGCA CGACCTGGAC 
AATCTGCGCG AGCAGGTTGA GGAGGAGGCT GAGGGCAAGG CCGATCTGCA GCGCCAGCTG 
AGCAAGGCCA ACGCTGAGGC CCAGGTGTGG CGCAGCAAGT ACGAGTCCGA TGGCGTTGCC 
CGCTCTGAGG AGCTGGAGGA AGCCAAGAGG AAGCTGCAGG CCCGTTTGGC CGAGGCCGAG 
GAGACCATCG AGTCCCTCAA CCAGAAGTGC ATTGGCCTGG AGAAGACCAA GCAGCGTCTG 
TCCACCGAGG TGGAGGATCT GCAGCTGGAG GTCGACCGTG CCAACGCCAT TGCCAACGCT 
GCCGAGAAGA AGCAGAAGGC CTTCGACAAG ATCATCGGCG AGTGGAAGCT CAAGGTCGAC 
GATCTGGCTG CTGAGCTGGA TGCCTCCCAG AAGGAGTGCC GCAACTACTC CACCGAGCTG 
TTCCGTCTTA AGGGCGCCTA CGAGGAGGGC CAGGAGCAGT TGGAGGCTGT GCGTCGTGAG 
AACAAGAACC TGGCCGATGA GGTCAAGGAT CTGCTCGACC AGATCGGTGA GGGTGGCCGC 
AACATCCATG AGATCGAGAA GGCCCGCAAG CGCCTGGAAG CCGAGAAGGA CGAGCTCCAG 
GCTGCCCTCG AGGAGGCTGA GGCCGCTCTT GAGCAGGAGG AGAACAAGGT GCTCCGCGCT 
CAGCTTGAGC TGTCCCAGGT GCGCCAGGAG ATCGACCGCC GCATCCAGGA GAAGGAGGAG 
GAGTTCGAGA ACACCCGCAA GAACCACCAG CGTGCCCTCG ACTCCATGCA GGCTTCCCTC 
GAAGCCGAGG CCAAGGGCAA GGCTGAGGCC CTGCGCATGA AGAAGAAGCT GGAGGCTGAC 
ATCAACGAGC TTGAGATTGC TCTGGATCAC GCCAACAAGG CTAACGCCGA GGCCCAGAAG 
AACATCAAGC GTTACCAGCA GCAGCTGAAG GACATCCAGA CTGCCCTCGA GGAGGAGCAG 
CGCGCCCGCG ACGATGCCCG CGAACAGCTG GGTATCTCCG AGCGTCGTGC AAACGCCCTC 
CAGAACGAAC TGGAGGAGTC TCGCACTCTG CTGGAACAGG CCGACCGTGG CCGTCGCCAG 
GCCGAACAGG AGCTGGCCGA TGCCCACGAG CAGCTGAACG AAGTGTCCGC CCAGAACGCC 
TCCATCTCCG CTGCCAAGAG GAAGCTGGAG TCCGAGCTGC AGACCCTGCA CTCCGACCTG 
GACGAACTCC TGAACGAAGC CAAGAACTCC GAGGAGAAGG CCAAGAAGGC TATGGTCGAT 
GCCGCCCGCC TGGCCGATGA GCTCCGCGCT GAGCAGGATC ATGCCCAGAC CCAGGAGAAA 
TTGAGGAAGG CCCTCGAGCA GCAGATCAAG GAGCTGCAGG TCCGTCTGGA CGAGGCTGAG 
GCCAACGCCC TCAAGGGAGG CAAGAAGGCC ATTCAGAAGC TTGAGCAGCG CGTCCGCGAG 
CTCGAGAACG AGCTGGATGG TGAGCAGAGG AGGCACGCCG ATGCCCAGAA GAACCTGCGC 
AAGTCCGAGC GTCGCGTCAA GGAGCTGAGC TTCCAGTCCG AGGAGGACCG CAAGAACCAC 
GAGCGCATGC AGGATCTGGT CGACAAGCTG CAACAGAAGA TCAAGACATA CAAGAGGCAG 
ATCGAGGAGG CTGAGGAAAT CGCCGCCCTC AACTTGGCCA AATTCCGCAA GGCTCAGCAG 
GAGCTTGAGG AGGCCGAGGA GCGCGCCGAT CTGGCCGAGC AGGCCATCAG CAAATTCCGC 
GCCAAGGGAC GTGCCGGTTC TGTCGGTCGT GGTGCCAGCC CAGCGCCCCG TGCGACGTCC 
GTTAGGCCAC AATTCGACGG ATTGGCCTTC CCACCAAGAT TCGACCTTGC TCCTGAAAAC 
GAATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mhc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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