Ccs cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccs
Entrez Gene ID	84485
Full Name	copper chaperone for superoxide dismutase
General protein information	Preferred Names copper chaperone for superoxide dismutase Names copper chaperone for superoxide dismutase superoxide dismutase copper chaperone
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	binds copper; acts as a chaperone to insert copper into the Cu, Zn superoxide dismutase Sod1 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053425.1	NP_445877.1	copper chaperone for superoxide dismutase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ccs	NP_445877.1
Danio rerio (zebrafish)	ccs	NP_001191151.1
	CCS	XP_001118002.2
Canis lupus familiaris (dog)	CCS	NP_001181899.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007026	XP_308747.4
Homo sapiens (human)	CCS	NP_005116.1
Rattus norvegicus (Norway rat)	LOC100911589	XP_003749080.1
Drosophila melanogaster (fruit fly)	CCS	NP_001163108.1
Saccharomyces cerevisiae (baker's yeast)	CCS1	NP_013752.1
Magnaporthe oryzae (rice blast fungus)	MGG_05281	XP_003712840.1
Arabidopsis thaliana (thale cress)	CCS	NP_563910.2
Oryza sativa (rice)	Os04g0573200	NP_001053613.1
Mus musculus (house mouse)	Ccs	NP_058588.1
Neurospora crassa	NCU07851	XP_963112.2
Xenopus tropicalis (tropical clawed frog)	ccs	NP_001011020.1
Pan troglodytes (chimpanzee)	CCS	NP_001167001.1
Kluyveromyces lactis	KLLA0F26917g	XP_456277.1

Gene Ontology
Bibliography

Related articles in PubMed

Copper delivery to the CNS by CuATSM effectively treats motor neuron disease in SOD(G93A) mice co-expressing the Copper-Chaperone-for-SOD.
Williams JR, Trias E, Beilby PR, Lopez NI, Labut EM, Bradford CS, Roberts BR, McAllum EJ, Crouch PJ, Rhoads TW, Pereira C, Son M, Elliott JL, Franco MC, Est?vez AG, Barbeito L, Beckman JS
Neurobiology of disease891-9(2016 May)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

BACE1 cytoplasmic domain interacts with the copper chaperone for superoxide dismutase-1 and binds copper.
Angeletti B, Waldron KJ, Freeman KB, Bawagan H, Hussain I, Miller CC, Lau KF, Tennant ME, Dennison C, Robinson NJ, Dingwall C
The Journal of biological chemistry280(18)17930-7(2005 May)

Cu,Zn-superoxide dismutase is lower and copper chaperone CCS is higher in erythrocytes of copper-deficient rats and mice.
West EC, Prohaska JR
Experimental biology and medicine (Maywood, N.J.)229(8)756-64(2004 Sep)

Copper, zinc-superoxide dismutase protein but not mRNA is lower in copper-deficient mice and mice lacking the copper chaperone for superoxide dismutase.
Prohaska JR, Geissler J, Brokate B, Broderius M
Experimental biology and medicine (Maywood, N.J.)228(8)959-66(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Beta-site amyloid precursor (APP) cleaving enzyme BACE1 expression reduces the activity of superoxide dismutase (SOD1) in cells consistent with direct competition for available CCS, as overexpression of CCS restores SOD1 activity.
Title: BACE1 cytoplasmic domain interacts with the copper chaperone for superoxide dismutase-1 and binds copper.

Associated with copper deficiency were higher levels of Ccs in erythrocytes.
Title: Cu,Zn-superoxide dismutase is lower and copper chaperone CCS is higher in erythrocytes of copper-deficient rats and mice.

Copper modulates the degradation of this protein, which prioritizes the utilization of copper when it is scarce.
Title: Copper modulates the degradation of copper chaperone for Cu,Zn superoxide dismutase by the 26 S proteosome.

The following Ccs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09530	NM_053425.1 Latest version!	Rattus norvegicus copper chaperone for superoxide dismutase (Ccs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccs ( NM_053425.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445877.1 Rattus norvegicus copper chaperone for superoxide dismutase (Ccs), mRNA.

Price & Availability↑

CloneID	ORa09530
Clone ID Related Accession (Same CDS sequence)	NM_053425.1
Accession Version	NM_053425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	copper chaperone for superoxide dismutase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF255305.1. ##Evidence-Data-START## Transcript exon combination :: AF255305.1, FQ219139.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCGA AGTCGGGGGA CGGTGGAACT ATGTGTGCGT TGGAGTTTAC AGTACAGATG 
AGTTGTCAGA GCTGCGTGGA CGCTGTGCAC AAGACCCTGA AAGGGGCGGC GGGTGTCCAG 
AATGTGGAAG TTCAGTTGGA GAACCAGATG GTGTTGGTGC AGACCACTTT GCCCAGCCAG 
GAGGTGCAAG CGCTCCTGGA AAGCACAGGG AGGCAGGCTG TACTCAAGGG CATGGGCAGC 
AGCCAACTAA AGAATCTGGG AGCAGCAGTG GCCATTATGG AGGGCAGTGG CACCGTACAG 
GGGGTGGTCC GCTTCCTACA GCTGTCCTCT GAGCTCTGCC TGATTGAGGG AACCATCGAC 
GGCCTGGAGC CTGGGCTGCA TGGGCTTCAT GTCCATCAGT ATGGGGACCT TACCAAGGAC 
TGCAGCAGCT GTGGGGACCA TTTTAACCCT GATGGAGCAT CTCATGGGGG TCCTCAGGAC 
ACTGATCGGC ACCGGGGAGA TCTGGGCAAT GTTCACGCTG AAGCTAGTGG CCGAGCTACC 
TTCCGGATAG AGGATAAACA GCTGAAGGTG TGGGATGTGA TTGGCCGCAG TCTGGTTGTT 
GATGAGGGAG AAGATGACCT GGGCCGGGGA GGCCATCCCT TATCCAAGGT CACAGGGAAT 
TCTGGGAAGA GGTTGGCCTG TGGCATCATT GCACGCTCTG CTGGCCTTTT CCAGAATCCC 
AAGCAGATCT GCTCCTGTGA TGGGCTCACT ATCTGGGAGG AGCGAGGCCG GCCCATTGCT 
GGCCAAGGCC GAAAGGACTC AGCCCAACCC CCTGCTCACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445877.1
CDS	52..876
Translation	MASKSGDGGTMCALEFTVQMSCQSCVDAVHKTLKGAAGVQNVEVQLENQMVLVQTTLPSQEVQALLESTGRQAVLKGMGSSQLKNLGAAVAIMEGSGTVQGVVRFLQLSSELCLIEGTIDGLEPGLHGLHVHQYGDLTKDCSSCGDHFNPDGASHGGPQDTDRHRGDLGNVHAEASGRATFRIEDKQLKVWDVIGRSLVVDEGEDDLGRGGHPLSKVTGNSGKRLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGQGRKDSAQPPAHL

Target ORF information:

RefSeq Version	NM_053425.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus copper chaperone for superoxide dismutase (Ccs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053425.1

ORF Insert Sequence:

ATGGCTTCGA AGTCGGGGGA CGGTGGAACT ATGTGTGCGT TGGAGTTTAC AGTACAGATG 
AGTTGTCAGA GCTGCGTGGA CGCTGTGCAC AAGACCCTGA AAGGGGCGGC GGGTGTCCAG 
AATGTGGAAG TTCAGTTGGA GAACCAGATG GTGTTGGTGC AGACCACTTT GCCCAGCCAG 
GAGGTGCAAG CGCTCCTGGA AAGCACAGGG AGGCAGGCTG TACTCAAGGG CATGGGCAGC 
AGCCAACTAA AGAATCTGGG AGCAGCAGTG GCCATTATGG AGGGCAGTGG CACCGTACAG 
GGGGTGGTCC GCTTCCTACA GCTGTCCTCT GAGCTCTGCC TGATTGAGGG AACCATCGAC 
GGCCTGGAGC CTGGGCTGCA TGGGCTTCAT GTCCATCAGT ATGGGGACCT TACCAAGGAC 
TGCAGCAGCT GTGGGGACCA TTTTAACCCT GATGGAGCAT CTCATGGGGG TCCTCAGGAC 
ACTGATCGGC ACCGGGGAGA TCTGGGCAAT GTTCACGCTG AAGCTAGTGG CCGAGCTACC 
TTCCGGATAG AGGATAAACA GCTGAAGGTG TGGGATGTGA TTGGCCGCAG TCTGGTTGTT 
GATGAGGGAG AAGATGACCT GGGCCGGGGA GGCCATCCCT TATCCAAGGT CACAGGGAAT 
TCTGGGAAGA GGTTGGCCTG TGGCATCATT GCACGCTCTG CTGGCCTTTT CCAGAATCCC 
AAGCAGATCT GCTCCTGTGA TGGGCTCACT ATCTGGGAGG AGCGAGGCCG GCCCATTGCT 
GGCCAAGGCC GAAAGGACTC AGCCCAACCC CCTGCTCACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Copper delivery to the CNS by CuATSM effectively treats motor neuron disease in SOD(G93A) mice co-expressing the Copper-Chaperone-for-SOD.
Neurobiology of disease891-9(2016 May)
Williams JR,Trias E,Beilby PR,Lopez NI,Labut EM,Bradford CS,Roberts BR,McAllum EJ,Crouch PJ,Rhoads TW,Pereira C,Son M,Elliott JL,Franco MC,Est?vez AG,Barbeito L,Beckman JS

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

BACE1 cytoplasmic domain interacts with the copper chaperone for superoxide dismutase-1 and binds copper.
The Journal of biological chemistry280(18)17930-7(2005 May)
Angeletti B,Waldron KJ,Freeman KB,Bawagan H,Hussain I,Miller CC,Lau KF,Tennant ME,Dennison C,Robinson NJ,Dingwall C

Cu,Zn-superoxide dismutase is lower and copper chaperone CCS is higher in erythrocytes of copper-deficient rats and mice.
Experimental biology and medicine (Maywood, N.J.)229(8)756-64(2004 Sep)
West EC,Prohaska JR

Copper, zinc-superoxide dismutase protein but not mRNA is lower in copper-deficient mice and mice lacking the copper chaperone for superoxide dismutase.
Experimental biology and medicine (Maywood, N.J.)228(8)959-66(2003 Sep)
Prohaska JR,Geissler J,Brokate B,Broderius M